EP 4209785 A1 20230712 - AKR1C3 DETECTION METHOD, AND DIAGNOSTIC KIT FOR DETECTING AKR1C3 AND USE THEREOF
Title (en)
AKR1C3 DETECTION METHOD, AND DIAGNOSTIC KIT FOR DETECTING AKR1C3 AND USE THEREOF
Title (de)
AKR1C3-NACHWEISVERFAHREN UND DIAGNOSTISCHER KIT ZUM NACHWEIS VON AKR1C3 UND VERWENDUNG DAVON
Title (fr)
PROCÉDÉ DE DÉTECTION D'AKR1C3 ET KIT DE DIAGNOSTIC POUR LA DÉTECTION D'AKR1C3 ET SON UTILISATION
Publication
Application
Priority
- CN 202010911697 A 20200902
- CN 2021114774 W 20210826
Abstract (en)
An AKR1C3 detection method, and a diagnostic kit for detecting AKR1C3 and use thereof. The AKR1C3 detection method comprises the following steps: treating a formalin-fixed paraffin-embedded human tissue specimen sequentially using an organic solvent, an alcohol, and water; performing antigen retrieval on the treated formalin-fixed paraffin-embedded human tissue specimen in the presence of an antigen retrieval solution; co-incubating the antigen-retrieved formalin-fixed paraffin-embedded human tissue specimen with a blocking buffer to block a non-specific antigen; mixing the blocked formalin-fixed paraffin-embedded human tissue specimen with a certain concentration of AKR1C3 monoclonal antibody solution for primary antibody incubation, and mixing the primary antibody-incubated formalin-fixed paraffin-embedded human tissue specimen with a certain concentration of a secondary antibody solution for secondary antibody incubation. The AKR1C3 detection method can be applied to the detection of AKR1C3 expression levels in various cancer tumor tissues and is stable in dyeing results, and has good sensitivity, precision and consistency.
IPC 8 full level
G01N 33/574 (2006.01); G01N 33/68 (2006.01)
CPC (source: EP IL KR US)
C07K 16/40 (2013.01 - US); G01N 33/573 (2013.01 - KR US); G01N 33/574 (2013.01 - IL); G01N 33/57484 (2013.01 - EP); G01N 33/577 (2013.01 - KR); G01N 33/68 (2013.01 - IL); G01N 2333/90206 (2013.01 - EP KR); G01N 2474/20 (2021.08 - US)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
EP 4209785 A1 20230712; AU 2021337711 A1 20230316; BR 112023003973 A2 20230411; CA 3192258 A1 20220310; CN 115485560 A 20221216; IL 300833 A 20230401; JP 2023540283 A 20230922; KR 20230058507 A 20230503; TW 202210833 A 20220316; US 2024142453 A1 20240502; WO 2022048492 A1 20220310
DOCDB simple family (application)
EP 21863557 A 20210826; AU 2021337711 A 20210826; BR 112023003973 A 20210826; CA 3192258 A 20210826; CN 2021114774 W 20210826; CN 202180031349 A 20210826; IL 30083323 A 20230221; JP 2023514086 A 20210826; KR 20237011169 A 20210826; TW 110131711 A 20210826; US 202118043610 A 20210826