EP 4284941 A1 20231206 - DETECTING BETA-LACTAMASE ENZYME ACTIVITY
Title (en)
DETECTING BETA-LACTAMASE ENZYME ACTIVITY
Title (de)
NACHWEIS VON BETA-LACTAMASE-ENZYMAKTIVITÄT
Title (fr)
DETECTION D'UNE ACTIVITE ENZYMATIQUE DES BÉTA-LACTAMASES
Publication
Application
Priority
- FR 2100708 A 20210126
- FR 2022050133 W 20220125
Abstract (en)
[origin: WO2022162303A1] It is essential to have efficient, simple, quick and transportable tools for reliably identifying bacteria that are multiresistant to antibiotics, more specifically broad-spectrum beta-lactamase (BSBL)-producing Enterobacteriaceae, which are the most widespread among Enterobacteriaceae. The present invention meets this requirement through its ease of use and its speed. The invention is based on detecting the enzyme activity of beta-lactam hydrolysis using an antibody capable of discriminating between the intact form of the beta-lactam ring of a beta-lactam and its hydrolysis product. This antibody can be used in kits and methods enabling for rapidly detecting (in less than one hour), without using expensive equipment (a small strip visible to the naked eye), the presence of bacteria producing penicillin-type, plasmid-mediated or hyper-produced AmpC enzymes, of BSBL or carbapenemase from colonies or in a sample.
IPC 8 full level
C12Q 1/10 (2006.01); C12Q 1/34 (2006.01); G01N 33/543 (2006.01); G01N 33/558 (2006.01); G01N 33/569 (2006.01); G01N 33/577 (2006.01)
CPC (source: EP US)
C12Q 1/10 (2013.01 - EP); C12Q 1/18 (2013.01 - US); C12Q 1/34 (2013.01 - EP US); G01N 33/54388 (2021.08 - EP); G01N 33/558 (2013.01 - EP); G01N 33/56916 (2013.01 - EP); G01N 33/577 (2013.01 - EP); C12Y 305/02006 (2013.01 - US); Y02A 50/30 (2017.12 - EP)
Citation (search report)
See references of WO 2022162303A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
WO 2022162303 A1 20220804; CN 117015613 A 20231107; EP 4284941 A1 20231206; JP 2024504411 A 20240131; US 2024110220 A1 20240404
DOCDB simple family (application)
FR 2022050133 W 20220125; CN 202280022705 A 20220125; EP 22706069 A 20220125; JP 2023544675 A 20220125; US 202218262523 A 20220125