EP 4294568 A1 20231227 - MICROFLUIDIC DEVICE FOR THE DIGESTION OF TISSUES INTO CELLULAR SUSPENSIONS
Title (en)
MICROFLUIDIC DEVICE FOR THE DIGESTION OF TISSUES INTO CELLULAR SUSPENSIONS
Title (de)
MIKROFLUIDISCHE VORRICHTUNG ZUR VERDAUUNG VON GEWEBE ZU ZELLSUSPENSIONEN
Title (fr)
DISPOSITIF MICROFLUIDIQUE POUR LA DIGESTION DE TISSUS EN SUSPENSIONS CELLULAIRES
Publication
Application
Priority
- US 202117180711 A 20210219
- US 2022016855 W 20220217
Abstract (en)
[origin: WO2022178168A1] A microfluidic device uses hydrodynamic shear forces on a sample to improve the speed and efficiency of tissue digestion is disclosed. The microfluidic channels are designed to apply hydrodynamic shear forces at discrete locations on tissue specimens up to 1 cm in length and 1 mm in diameter, thereby accelerating digestion through hydrodynamic shear forces and improved enzyme-tissue contact. The microfluidic digestion device can eliminate or reduce the need to mince tissue samples with a scalpel, while reducing sample processing time and preserving cell viability. Another advantage is that downstream microfluidic operations could be integrated to enable advanced cell processing and analysis capabilities. The device may be used in research and clinical settings to promote single cell-based analysis technologies, as well as to isolate primary, progenitor, and stem cells for use in the fields of tissue engineering and regenerative medicine.
IPC 8 full level
B01L 3/00 (2006.01); C12M 1/00 (2006.01); C12N 15/10 (2006.01)
CPC (source: EP KR)
B01L 3/502746 (2013.01 - KR); B01L 3/502753 (2013.01 - EP KR); C12M 23/16 (2013.01 - EP KR); C12M 45/02 (2013.01 - EP KR); B01L 3/502746 (2013.01 - EP); B01L 2200/0647 (2013.01 - EP KR); B01L 2300/0681 (2013.01 - EP KR); B01L 2300/0816 (2013.01 - EP KR); B01L 2300/0864 (2013.01 - EP KR); B01L 2400/0487 (2013.01 - EP KR); B01L 2400/086 (2013.01 - EP)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
WO 2022178168 A1 20220825; CA 3211524 A1 20220825; EP 4294568 A1 20231227; JP 2024506928 A 20240215; KR 20230146599 A 20231019
DOCDB simple family (application)
US 2022016855 W 20220217; CA 3211524 A 20220217; EP 22756955 A 20220217; JP 2023549574 A 20220217; KR 20237031470 A 20220217