EP 4295158 A1 20231227 - METHODS FOR PEPTIDE MAPPING OF ADENO-ASSOCIATED VIRUS (AAV) PROTEINS
Title (en)
METHODS FOR PEPTIDE MAPPING OF ADENO-ASSOCIATED VIRUS (AAV) PROTEINS
Title (de)
VERFAHREN ZUR PEPTIDKARTIERUNG VON ADENO-ASSOZIIERTEN VIRUS (AAV)-PROTEINEN
Title (fr)
PROCÉDÉS DE CARTOGRAPHIE PEPTIDIQUE DE PROTÉINES DU VIRUS ADÉNO-AS ASSOCIÉ (VAA)
Publication
Application
Priority
- US 202163151366 P 20210219
- IB 2021053914 W 20210507
Abstract (en)
[origin: US2022268783A1] The present disclosure relates to a method of characterizing proteins in a sample. The method includes: removing non-ionic surfactant from the sample via denaturing size-exclusion chromatography to form a denatured sample; eluting the denatured sample via liquid chromatography to collect fractions of the sample, wherein the fractions of the sample include a protein fraction; lyophilizing the protein fraction to increase protein concentration; reconstituting the lyophilized protein fraction with a buffer comprising a surfactant to denature the protein; digesting the denatured protein fraction with an enzyme; and analyzing the digested protein fraction.
IPC 8 full level
G01N 33/68 (2006.01)
CPC (source: EP US)
G01N 30/20 (2013.01 - US); G01N 30/22 (2013.01 - US); G01N 30/7233 (2013.01 - US); G01N 33/6848 (2013.01 - EP US); G01N 33/6851 (2013.01 - EP); G01N 2030/027 (2013.01 - US)
Citation (search report)
See references of WO 2022175728A1
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
US 2022268783 A1 20220825; CN 116848413 A 20231003; EP 4295158 A1 20231227; WO 2022175728 A1 20220825
DOCDB simple family (application)
US 202117314452 A 20210507; CN 202180094187 A 20210507; EP 21729640 A 20210507; IB 2021053914 W 20210507