EP 4317208 A1 20240207 - AMINATION METHOD FOR POLYSTYRENE RESIN, AND METHOD FOR IMMOBILIZING ENZYME USING AMINATED RESIN
Title (en)
AMINATION METHOD FOR POLYSTYRENE RESIN, AND METHOD FOR IMMOBILIZING ENZYME USING AMINATED RESIN
Title (de)
AMINIERUNGSVERFAHREN FÜR POLYSTYROLHARZ UND VERFAHREN ZUR IMMOBILISIERUNG EINES ENZYMS MIT AMINIERTEM HARZ
Title (fr)
PROCÉDÉ D'AMINATION D'UNE RÉSINE DE POLYSTYRÈNE ET PROCÉDÉ D'IMMOBILISATION D'UNE ENZYME À L'AIDE D'UNE RÉSINE AMINÉE
Publication
Application
Priority
- CN 202110329787 A 20210329
- CN 2021092799 W 20210510
Abstract (en)
Provided is an amination method for a polystyrene-type resin, and a method for immobilizing an enzyme using an aminated polystyrene-type resin. The amination method includes: in a solvent, a catalyst is used to catalyze a polystyrene-type resin and an enamine salt to perform a Friedel-Crafts alkylation reaction, to obtain an aminated polystyrene-type resin, herein the catalyst is a Lewis acid catalyst. By means of the Friedel-Crafts alkylation reaction, the enamine salt is grafted onto the polystyrene-type resin, and the polystyrene-type resin is aminated. The conditions of the Friedel-Crafts alkylation reaction are easy to control, the post-treatment process is simple, and it is only necessary to remove the catalyst and the unreacted enamine salt by washing. Therefore, the above amination method in the present application has few steps and is simple and easy to implement. Meanwhile, the amination method further avoids the use of a noble metal catalyst, thereby reducing production cost. When the obtained polystyrene resin is used as an enzyme immobilization carrier, good immobilization effect and usability are achieved. This method also provides a new class of enzyme immobilization carriers to the current library.
IPC 8 full level
C08F 8/44 (2006.01); C08F 112/08 (2006.01); C12N 11/082 (2020.01)
CPC (source: CN EP KR US)
C08F 8/32 (2013.01 - US); C08F 8/44 (2013.01 - CN EP KR); C08F 112/08 (2013.01 - US); C08F 212/08 (2013.01 - KR); C08F 212/36 (2013.01 - KR); C12N 9/1096 (2013.01 - CN EP KR); C12N 11/06 (2013.01 - KR); C12N 11/08 (2013.01 - EP); C12N 11/082 (2020.01 - CN KR US); C08F 212/08 (2013.01 - EP); C08F 212/36 (2013.01 - EP); C08F 2810/20 (2013.01 - EP); Y02P 20/50 (2015.11 - EP)
C-Set (source: CN EP)
CN
EP
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
EP 4317208 A1 20240207; CN 112707977 A 20210427; CN 112707977 B 20210702; JP 2024513372 A 20240325; KR 20230162102 A 20231128; US 2024191008 A1 20240613; WO 2022205565 A1 20221006
DOCDB simple family (application)
EP 21934216 A 20210510; CN 2021092799 W 20210510; CN 202110329787 A 20210329; JP 2023559817 A 20210510; KR 20237037467 A 20210510; US 202118552808 A 20210510