EP 4351777 A1 20240417 - APPARATUS AND METHOD FOR CONTINUOUS PRODUCTION OF RNA
Title (en)
APPARATUS AND METHOD FOR CONTINUOUS PRODUCTION OF RNA
Title (de)
VORRICHTUNG UND VERFAHREN ZUR KONTINUIERLICHEN HERSTELLUNG VON RNA
Title (fr)
APPAREIL ET PROCÉDÉ DE PRODUCTION CONTINUE D'ARN
Publication
Application
Priority
- US 202163197551 P 20210607
- US 2022032436 W 20220607
Abstract (en)
[origin: WO2022261050A1] A modular apparatus for continuous production of purified RNA, the apparatus including, in operable communication: a reaction module including a bioreactor configured for continuous synthesis of an affinity-tagged RNA molecule by in vitro transcription using reactants in an in vitro transcription mixture; a control module including a sensor; and an RNA separation system configured to receive a transcription reaction product comprising the affinity-tagged RNA molecule from the bioreactor and to separate the affinity tagged RNA molecule from the transcription reaction product, wherein the RNA separation system includes a capture module including at least one affinity column for binding the affinity-tagged RNA molecule and a purification module including a continuous chromatography column, wherein the control module controls input of the reactants to the bioreactor and monitors separation of the affinity-tagged RNA molecule using the sensor, and wherein the sensor is in communication with the reaction module and the RNA separation system and the sensor provides feedback control of the transcription mixture in the reaction module and the transcription reaction product in the RNA separation system.
IPC 8 full level
B01J 19/00 (2006.01); B01D 15/38 (2006.01); C12N 15/10 (2006.01); C12P 19/34 (2006.01)
CPC (source: EP)
B01D 15/3804 (2013.01); C12N 15/101 (2013.01); C12P 19/34 (2013.01); C12Y 207/07006 (2013.01)
Designated contracting state (EPC)
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated extension state (EPC)
BA ME
Designated validation state (EPC)
KH MA MD TN
DOCDB simple family (publication)
DOCDB simple family (application)
US 2022032436 W 20220607; EP 22820865 A 20220607