(19)
(11)EP 0 000 035 A1

(12)EUROPEAN PATENT APPLICATION

(43)Date of publication:
20.12.1978 Bulletin 1978/01

(21)Application number: 78100058.3

(22)Date of filing:  01.06.1978
(51)International Patent Classification (IPC)2C07C 101/77, A61K 31/195, A61K 31/215
(84)Designated Contracting States:
BE CH DE FR GB LU NL SE

(30)Priority: 01.06.1977 US 802390

(71)Applicant: Merck & Co., Inc.
Rahway New Jersey 07065-0900 (US)

(72)Inventors:
  • Patchett, Arthur Allan
    Cranford, N.J. (US)
  • Taub, David
    Metuchen, N.J. (US)

(74)Representative: Abitz, Walter, Dr.-Ing. et al
Patentanwälte Abitz & Partner Postfach 86 01 09
81628 München
81628 München (DE)


(56)References cited: : 
  
      


    (54)Alpha-amino acids, compositions and process for preparing said compounds


    (57) a-Amino Acids, compositions and process for preparing said compounds a-ethynyl- and - a-vinyl- 3,4- disubstituted pheynylalanines and esters thereof, have been prepared by reaction of methyl 3,4-dihydroxybenzoate with diphenyldichloromethane, reduction of the so obtained methyl 3,4-diphenylmethylene- dioxy- benzoate to 3,4-diphenylmethylenedioxybenzyl- alcohol, and transforming this to the benzylchloride. This 3,4-diphenylmethylenedioxybenzylchloride reacts with 1-trimethyl- silyl-N- benzylidene-3-aminoprop-1-yneto form 3-(3,4- diphenylmethylenedioxybenzyl) -1-trimethysilyl N-benzylidene-3- aminoprop-1-yne.
    With methyl chloroformate this compound gives 3-carbomethoxy-3- (3,4- diphenylmethylenedioxybenzyl-1-trimethylsilyl -N-benzylidene -3-aminoprop-1-yne.
    The benzylidene-group, the trimethylsilyl-group and the diphenyl-methylene- group are removed, which results in the formation of a-ethynyl- 3,4-dihydroxy- phenylalanine.
    The a-vinyl- analog is obtained by hydrogenation of an intermediate. The compounds have a pharmaceutical activity.


    Description

    BACKGROUND OF THE INVENTION



    [0001] The present invention is concerned with a-ethynyl-and a-vinyl-3,4-disubstituted phenylalanines and especially the 3,4-dihydroxyphenyla- lanine species.

    [0002] a-Methyl-3,4-dihydroxyphenylalanine, particularly its L-isomer, is a known antihypertensive agent. (U.S. 2,868,818; U.S. 3,344,023).

    [0003] Novel a-ethynyl-and a-vinyl-3,4-disubstituted phenylalanines have been discovered. These novel alanines have pharmaceutical activity including antihypertensive action.

    SUMMARY OF THE INVENTION



    [0004] a-Ethynyl-and a-vinyl-3,4-di-OR-phenylalanines, esters thereof and their pharmaceutical use.

    DESCRIPTION OF THE PREFERRED EMBODIMENTS



    [0005] The present invention is embodied in a-ethynyl or a-vinyl phenylalanine compounds having the formula

    wherein

    L is -CaCH or -CH=CH2,

    R1 and R2 are independently selected from H and C2-C6 alkanoyl, and

    R is C1-C18 alkyl or H.



    [0006] The pharmaceutically acceptable salts of the formula I compounds are also included. These salts generally are acid addition salts of suitable organic or inorganic acids. Preferred salts are the hydrohalides such as the hydrobromides, the hydrochlorides, the hydrogen iodides. Most preferred salts are the hydrochlorides.

    [0007] The compounds of formula I have a chiral center and may occur in optically active forms, i.e., as optical isomers. These isomers are conventionally designated as D and L, d and 1, + and -, (S) and (R) or by a combination of these symbols. Where the compound name or formula does not specify the isomer form, all forms are included, i.e., the individual isomers, mixtures thereof and racemates.

    [0008] Of the isomers, the L-form is preferred.

    [0009] R may be H or an alkyl group, preferably a C1-C18 alkyl group. Examples of suitable alkyl groups are octadecyl, 2-ethylhexyl, lauryl, undecyl, methyl, isopropyl, hexyl and the like. Preferred R groups are H and Cl-C6 alkyl. Most preferred R groups are H and ethyl.

    [0010] R1 and R2 include H and C2-C6 alkanoyl groups. Examples of suitable alkanoyl groups are acetyl, octanoyl, pivaloyl, 2-methylpropanoyl, heptanoyl, butanoyl and the like. The most preferred Rl/R2 substituent is hydrogen.

    [0011] A preferred class of compounds of the present invention is that having the formula



    [0012] Especially preferred are formula II compounds where R is hydrogen or C1-C6 alkyl, preferably ethyl.

    [0013] The L-isomer form of the formula II compound is also more preferred.

    [0014] Another preferred class of compounds of the present invention is that having the formula



    [0015] Especially preferred are the formula III compounds where R is hydrogen or C3-C6 alkyl, preferably ethyl.

    [0016] The L-isomer form of the formula III compounds are also more preferred.

    [0017] The compounds of the present invention have pharmaceutical activity especially as antihypertensive agents. Thus, the present compounds are useful for treating hypertension in humans.

    [0018] Other biological activities of the present compounds include inhibition of 3,4-dihydroxyphenyla- lanine (dopa) decarboxylase.

    [0019] For treating hypertension, the present compounds may be administered to the hypertensive patient orally, parenterally or via any other suitable administration route. Conventional dosage forms are used such as tablets, troches, capsules, liquid formulations, e.g., solutions, dispersions, emulsiions, elixirs and the like. Conventional compounding ingredients, i.e., diluents, carriers, etc. and conventional preparation procedures are utilized.

    [0020] The daily dosage of the present compounds may be varied as required. In general, a daily dosage range for the hypertensive patient is about 50 mg. to about 5000 mg. A preferred daily dosage range is about 100 mg. to about 3500 mg. A more preferred daily dosage range is about 250 to about 1500 mg.

    [0021] Compounds of the present invention may be prepared by any convenient process.

    [0022] An especially useful process for preparing the compounds of formula I where R is hydrogen is by the hydrolysis of a compound having the formula

    where L is -C=CH or -CH=CH2, R' is alkyl preferably C1-C6 alkyl, and R3 and R4 are groups, e.g., H, CH3 or phenyl, which permit hydrolysis of the dioxy moiety. The hydrolysis is carried out using conventional reagents and conditions, for example using an acid such as HCl, HBr, H3PO4, in a suitable solvent such as water, aqueous alkanols and the like. The hydrolysis may be carried out at room temperature or at elevated temperatures up to about 140°C. The reaction time will vary depending on other parameters such as temperature, etc.

    [0023] Where R in formula I is an alkyl group, the compound is prepared by conventional esterification of the corresponding compound where R is H as illustrated by the following equation



    [0024] The pharmaceutically acceptable salts of the present compounds may be obtained directly from the hydrolysis reaction described above. Such salts may also be obtained by treatment of the formula I free base with an appropriate acid under suitable conditions.

    [0025] Where the compounds of the present invention are obtained as racemates, they may be separated into the individual enantiomers by conventional resolution techniques. Such techniques commonly involve the formation of salts of the present racemeic acids with optically active bases. The resolution is preferably carried out on the O,O,N-triacyl derivatives of the racemic acid mixture. These acyl derivatives are prepared by treatment of the free acid mixture with a suitable acylating agent as illustrated by the following equation:



    [0026] The resolution procedure including hydrolysis of the resolved acylated acids is exemplified in U.S. 3,344,023.

    [0027] Compounds of the present invention where R1 and R2 are lower alkanoyl are prepared by appropriately acylating the corresponding compound where R and R2 are each H, as illustrated by the following equation:



    [0028] To prevent acylation of the a-NH2 group, the reaction may be carried out in an acid medium, e.g., glacial acetic acid. An example illustrating such an acylation system is in U.S. 3,983,138.

    [0029] The following examples illustrate preparation of compounds of the present invention via a series of intermediate steps. All temperatures are °C. The symbol Ph represents the phenyl group in the formulae below:



    [0030] A mixture of methyl 3,4-dihydroxybenzoate (8.40 g.; 50 mmol) and diphenyldichloromethane was stirred at 150 + 5° for 15 minutes. The mixture was cooled, taken up in benzene and the benzene solution washed with 5% aqueous KHCO3, saturated aqueous NaCl, dried over MgSO4 and concentrated to dryness. The crystalline residue (m.p. 98-100°) was recrystallized from hexane containing a little benzene to give pure methyl 3,4-diphenyl- methylenedioxybenzoate (15.3 g., 96%) m.p. 103-105°.

    [0031] 



    [0032] To a stirred suspension of 1.48 g. LiAlH4 in 80 ml. of ether was added dropwise a solution of methyl 3,4-diphenylmethylenedioxy- benzoate (13.09 g.; 39.4 mmol) in 80 ml. ether and 10 ml. tetrahydrofuran. The rate of addition was controlled to maintain the reaction mixture at a gentle reflux. The mixture was then refluxed 45 minutes. It was then cooled and 5 ml of ethyl acetate was added dropwise followed by 15 ml. of saturated aqueous Na2SO4 and about 5 g. anhydrous MgS04. The mixture was filtered, the inorganic precipitate washed with 1:1 ether-benzene, and the combined filtrate and washings concentrated to dryness to give 11.65 g. of 3,4-diphenylmethylenedioxybenzyl alcohol as a colorless viscous oil which partially solidified on cooling. On recrystallization from hexane-benzene an aliquot had m.p. 63-64°.

    [0033] 



    [0034] To a stirred solution of 11.6 g. (38 mmol) of 3,4-diphenylmethylenedioxybenzyl alcohol in ether (80 ml.) and pyridine (0.6 ml.) at 20°C. was added dropwise a solution of SOC12 in ether (40 ml.). The mixture was cooled to 0-5°C., and CH2Cl2 and water were added. The layers were separated, and the organic layer was washed with water and saturated aqueous NaCl, dried over MgSO4 and concentrated to dryness to yield 3,4-diphenylmethylenedioxybenzyl chloride (11.43 g.) as a colorless viscous oil: tlc (silica gel CH2C12: Rf0.8; ir (CC14) no -OH-, nmr (CCl4) δ 4.34 (s, 2H), 6.74 (d,j=8) 2H, 6.67 (s, 1H), 7.1-7.6 (m, 10H).



    [0035] To a stirred solution of 1-trimethylsilyl-N-benzylidene-3-aminoprop-l-yne (7.609 g.; 34.4 mmol) in 106 ml. of tetrahydrofuran maintained at -78° under N2 was added dropwise 19.5 ml. of 1.63 N n-butyl lithium. To the stirred deep red solution was then added dropwise 11.43 g. (35.4 mmol) of 3,4-diphenylmethylenedioxybenzyl chloride in 35 ml. of tetrahydrofuran. After an additional 30 minutes at -78° water (25 ml.) was added dropwise, the mixture was warmed to 20°, 10% aqueous NH4Cl, solution was added and the layers were separated. The aqueous layer was washed twice with benzene, the combined organic phases were washed twice with cold 10% aqueous Nh4Cl, once with saturated NaCl solution, dried over Na2SO4 and concentrated to dryness to give 3-(3,4-diphenylmethylenedioxybenzyl)-l-trimethylsilyl-NN-benzylidene-3-aminoprop-l-yne as a viscous orange oil (18,86 g).

    [0036] The mass spectrum showed a large molecular ion peak at 501.



    [0037] To diisopropylamine (700 mg.) in 15 ml. tetrahydrofuran at -78° under N2 was added 3.8 ml. of 1.64 M n-butyllithium dropwise. After 5 minutes, 3-(3,4-diphenylmethylenedioxybenzyl)-1-trimethylsilyl-N-benzylidene-3-aminoprop-l-yne (3.378 g.; 6.74 mmol) in 15 ml. tetrahydrofuran was added dropwise (10 minutes). After an additional 5 minutes, methyl chloroformate (680 mg.) in tetrahydrofuran (10 ml.) was added dropwise (5 minutes). After 40 minutes at -78° the solution was warmed to 0° and the color lightened from deep red to orange. After 10 minutes at 0°, water (5 ml.) was added dropwise followed by 10% aqueous NH4Cl (30 ml.). The layers were separated and the mixture extracted as in the previous example, dried over Na2SO4 and concentrated to dryness to give 3-carbomethoxy-3-(3,4-diphenylmethylenedioxy- benzylil-trimethylsilyl-N-benzylidene-3-aminoprop-1-yne (3.645 g.) as an orange foam; mass spectrum M/e 559, large fragmentation peaks at 287 (base peak) and 272.



    [0038] Chromatography of crude 3-carbomethoxy-3-(3,4-diphenylmethylenedioxybenzyl) 1-trimethylsilyl-N-benzylidene-3-aminoprop-1-yne (3.55 g.) over 180 g. of silica gel H eluting with 2% acetone in chloroform led to hydrolysis of the Schiff base protecting group to give the free amine, 3-carbomethoxy-3-(3,4-diphenylmethylenedioxybenzyl)-3-aminprop-l-yne (tlc silica gel 3% acetone in CHC13 RF~.2) Mass spec. M/e 471.



    [0039] To a solution of 3-carbomethoxy-3-(3,4-diphenylmethylenedioxybenzyl)-1-trimethylsilyl-N-benzylidine-3-aminoprop-l-yne (610 mg.) in 6 ml. of methanol under nitrogen at 20°C was added. 1.1 ml. of 1.6 M NaOCH3 in CH3OH. The solution was stirred 30 minutes, CH2C12 and cold water were added and the layers separated. The aqueous layer was washed with CH2Cl2 and the combined CH2Cl2 phase washed with cold water and saturated NaCl solution, dried over Na2SO4 and concentrated to dryness to give 3-carbomethoxy-3-(3,4-diphenylmethylenedioxybenzyl)-3-aminoprop-l-yne (505 mg.) as a viscous orange foam; nmr (CDC13) 1.80 (broad S,2H) 2.45 (s, 1H) 3.10 (s,2H), 3.75 - (s,3H) 6.79(d,j=5,2H), 6.75 (s, 1H), 7.0-7.7 (m, 10H).



    [0040] A solution of 3-carbomethoxy-3-(3,4-diphenylmethylenedioxybenzyl)-3-aminoprop-l-yne (230 mg.) in 6N HC1 (15 ml.) was refluxed for 2 hours, cooled and extracted with CH2Cl2. The aqueous acid phase was taken to dryness to give a-ethynyl-3,4-dihydroxyphenylalanine hydrochloride (135 mg, 90%) tlc-n butanol :acetic acid: water 25:4:10 single spot RF 0.4; n-butanol:acetic acid:water:pyridine 15:3:12:10 single spot RF 0.6; mass spec. M+ 221; (D2O) δ 3.17 (s,2H), 3.22 (s,lH) 6.70 m,3H) .

    [0041] The HCl salt obtained in Example 1 H.) may be conventionally neutralized or treated with an HC1 scavenger such as propylene oxide to obtain the corresponding free amino acid.

    EXAMPLE 2



    [0042] 



    [0043] A solution of 3-carbomethoxy-3-(3,4-diphenylmethylenedioxybenzyl-3-aminoprop-1-yne (90 mg.) in 10 ml. of ethyl acetate and 0.02 ml. quinoline was stirred in 1 atm of hydrogen over 20 mg. of Lindlar's catalyst [5%Pd-CaCO3 + Pb(OAc)2] at 25° until hydrogen uptake ceased. The catalyst was removed by filtration and the filtrate taken to dryness. The nearly pure product, was purified by dry column chromatography on 10 g. of filica gel eluting with 15% acetone in chloroform to give pure 3-carbomethoxy-3-(3,4-diphenylmethylenedioxybenzyl)-3-aminoprop-1-ene, mass spec. M+ 401; nmr (CDC13) 1.72 (s, 2H), 2.75 (d, J=14,1H),~ 3.18 (d,J=14.1H)-AB quartet 3.70 (s,3H), 5.17 (d,d,J=10,2,lH), 5.35 (d,d,J= 18,2,1H) 6.17 (d,d,J=18, 10, 1H) 6.72-677 (m, 3H); 7.25-7,8 (mk 10 H).


    Utilizing the procedure of Example 1



    [0044] H.), 3-carbomethoxy-3(3,4-diphenylmethylenedioxybenzyl)-3-aminoprop-l-ene was hydrolyzed to produce a-vinyl-3,4-dihydroxyphenylalanine hydrochloride. tlc-n-butanol, acetic acid:water: pyridine-15:3:12:10 single spot RF ~0.65; mass spec M+ 223:nmr(D20) δ 3.03 (d,J=15, 1H), 3,33(d,J=15, 1H) AB quartet, 5.35 (d,J=18, 1H); 5.50 (d,J=12, 1H), 6.18 (d,d,J= 18,12 1H), 6.6-7.1 (m 3H).

    [0045] The HC1 salt obtained in Example 2 B may be conventionally neutralized or treated with an HC1 scavenger such as propylene oxide to obtain the corresponding free amino acid.

    [0046] Claims to the invention.


    Claims

    1. Compounds having the formula

    wherein

    L is -C≡CH or -CH=CH2,

    R1 and R2 are independently selected from H and Cl-C6 alkanoyl, and

    R is C1-C18 alkyl or H.


     
    2. The pharmaceutically acceptable salts of the Claim 1 compounds.
     
    3. Compounds of Claim 1 having the L-isomer configuration.
     
    4. Compounds of Claim 1 wherein R1 and R2 are both hydrogen.
     
    5. Compounds of Claim 4 wherein R is hydrogen.
     
    6. Compounds of Claim 1 having the formula


     
    7. Compounds of Claim 6 having the L-isomer configuration.
     
    8. Compounds of Claim 6 wherein R1 and R2 are both hydrogen.
     
    9. Compounds of Claim 6 wherein R is H or C1-C6 alkyl.
     
    10. Compounds of Claim 8 wherein R is ethyl.
     
    11. Compounds of Claim 8 wherein R is hydrogen.
     
    12. Compounds of Claim 11 having the L-isomer configuration.
     
    13. Compounds of Claim 1 having the formula


     
    14. Compounds of Claim 13 having the L-isomer configuration.
     
    15. Compounds of Claim 13 wherein R1 and R2 are both hydrogen.
     
    16. Compounds of Claim 15 wherein R is H or Cl-C6 alkyl.
     
    17. Compounds of Claim 14 wherein R is ethyl.
     
    18. Compounds of Claim 16 wherein R is H.
     
    19. Compounds of Claim 18 having the L-isomer configuration.
     
    20. Pharmaceutical composition containing a therapeutical effective amount of a compound of Claim 1 or a pharmaceutically acceptable salt thereof.
     
    21. A process for preparing a compound having the formula

    which comprises hydrolysing a compound having the formula

    wherein

    L is -C≡CH or -CH=CH2,

    R is C1-C18 alkyl and

    R3 and R4 are independently selected from the group consisting of H, CH3 or a phenyl group.


     
    22. The process of Claim 21 wherein R is -CH3.
     
    23. The process of Claim 22 wherein L is -C≡CH.
     
    24. The process of Claim 22 wherein L is -CH=CH2.
     





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