(19) | |
| (11) | EP 2 302 043 A3 |
(12) | EUROPEAN PATENT APPLICATION |
(88) | Date of publication A3: | | 20.07.2011 Bulletin 2011/29 |
(43) | Date of publication A2: | | 30.03.2011 Bulletin 2011/13 |
(22) | Date of filing: 29.11.1999 |
| (51) | International Patent Classification (IPC): |
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(84) | Designated Contracting States: | | AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE |
(30) | Priority: | 27.11.1998 DK 157298 22.03.1999 DK 39199 15.10.1999 DK 148199
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(62) | Application number of the earlier application in accordance with Art. 76 EPC: | | 09165405.3 / 2113563 | | 99973065.8 / 1131416 |
(71) | Applicant: Novozymes A/S | | 2880 Bagsvaerd (DK) |
| (72) | Inventors: | | - Bojsen, Kirsten
2900 Hellerup (DK) - Svendsen, Allan
2970 Hoersholm (DK) - Fuglsang, Claus Crone
2990 Nivaa (DK) - Patkar, Shamkant Anant
2800 Kgs. Lyngby (DK) - Borch, Kim
3460 Birkeroed (DK) - Vind, Jesper
3500 Vaerloese (DK) - Petri, Andreas
2200 Copenhagen (DK) - Glad, Sanne Schroeder
2840 Holte (DK) - Budolfsen, Gitte
2000 Frederiksberg (DK)
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(54) | Lipolytic enzyme variants |
(57) The substrate specificity of a lipolytic enzyme can be modified by making alterations to the amino acid sequence in a defined region of the lipolytic enzyme, so as to increase the level of a desired activity or to decrease the level of an undesired activity. Thus, the inventors have developed lipolytic enzyme variants with a modified amino acid sequence with a substrate specificity which can be tailored for specific uses.