(19) | |
| (11) | EP 0 458 064 A3 |
(12) | EUROPEAN PATENT APPLICATION |
(88) | Date of publication A3: | | 17.06.1992 Bulletin 1992/25 |
(43) | Date of publication A2: | | 27.11.1991 Bulletin 1991/48 |
(22) | Date of filing: 18.04.1991 |
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(84) | Designated Contracting States: | | AT BE CH DE DK ES FR GB GR IT LI LU NL SE |
(30) | Priority: | 04.05.1990 US 519047
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(71) | Applicant: AMERICAN CYANAMID COMPANY | | Wayne, NJ 07470-8426 (US) |
| (72) | Inventors: | | - Buckwalter, Brian Lee
Yardley,
Pennsylvania (US) - Cady, Susan Mancini
Yardley,
Pennsylvania (US) - Daley, Michael Joseph
Yardley,
Pennsylvania (US) - Shieh, Hong-Ming
Langhorne,
Pennsylvania (US)
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(74) | Representative: Wächtershäuser, Günter, Prof. Dr. et al | | Patentanwalt,
Tal 29 80331 München 80331 München (DE) |
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(54) | Stabilization of somatotropins and other proteins by modification of cysteine residues |
(57) The present invention relates to physiologically-active derivatized natural and recombinant mammalian and human proteins and polypeptides. The invention provides chemical methods for derivatizing natural and recombinantly-derived proteins or polypeptides containing cysteine residues, either naturally or through site specific mutageneses. The pharmaceutical compositions containing said derivatized proteins and/or polypeptides are formulated to provide stable, long-acting compositions of such proteins and/or polypeptides, previously difficult to achieve.