(19)
(11)EP 1 572 111 B1

(12)EUROPEAN PATENT SPECIFICATION

(45)Mention of the grant of the patent:
29.04.2015 Bulletin 2015/18

(21)Application number: 03789842.6

(22)Date of filing:  18.11.2003
(51)International Patent Classification (IPC): 
C12N 15/12(2006.01)
A61K 38/57(2006.01)
C07K 14/81(2006.01)
A61L 15/44(2006.01)
(86)International application number:
PCT/US2003/037052
(87)International publication number:
WO 2004/060275 (22.07.2004 Gazette  2004/30)

(54)

WOUND CARE COMPOSITIONS

WUNDPFLEGEZUSAMMENSETZUNGEN

COMPOSITIONS SERVANT A TRAITER DES PLAIES


(84)Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR

(30)Priority: 19.12.2002 US 325446

(43)Date of publication of application:
14.09.2005 Bulletin 2005/37

(73)Proprietor: Avent, Inc.
Alpharetta, GA 30004 (US)

(72)Inventor:
  • QUIRK, Stephen
    Alpharetta, GA 30022 (US)

(74)Representative: Beacham, Annabel Rose et al
Dehns St Bride's House 10 Salisbury Square
London EC4Y 8JD
London EC4Y 8JD (GB)


(56)References cited: : 
EP-A- 0 648 838
US-A- 5 714 465
US-A1- 2002 086 420
EP-A1- 1 041 083
US-A- 5 914 392
US-A1- 2002 103 122
  
  • LIU YILIANG E ET AL: "Preparation and characterization of recombinant tissue inhibitor of metalloproteinase 4 (TIMP-4)" JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, no. 33, 1997, pages 20479-20483, XP002928483 ISSN: 0021-9258
  • LANGTON KEVIN P ET AL: "Localization of the functional domains of human tissue inhibitor of metalloproteinases-3 and the effects of a Sorsby's fundus dystrophy mutation" JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY OF BIOLOCHEMICAL BIOLOGISTS, BIRMINGHAM, US, vol. 273, no. 27, 3 July 1998 (1998-07-03), pages 16778-16781, XP002417706 ISSN: 0021-9258
  • HUANG WEN ET AL: "Folding and characterization of the amino-terminal domain of human tissue inhibitor of metalloproteinases-1 (TIMP-1) expressed at high yield in E. coli" FEBS LETTERS, vol. 384, no. 2, 1996, pages 155-161, XP002541863 ISSN: 0014-5793
  • BODDEN M KIRBY ET AL: "Functional domains of human TIMP-1 (tissue inhibitor of metalloproteinases)" JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 269, no. 29, 1994, pages 18943-18952, XP002542182 ISSN: 0021-9258
  • BUTLER GEORGINA S ET AL: "The specificity of TIMP-2 for matrix metalloproteinases can be modified by single amino acid mutations" JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 274, no. 29, 16 July 1999 (1999-07-16), pages 20391-20396, XP002542181 ISSN: 0021-9258
  • VAALAMO MAARIT ET AL: "Differential expression of tissue inhibitors of metalloproteinases (TIMP-1, -2, -3, and -4) in normal and aberrant wound healing" HUMAN PATHOLOGY, vol. 30, no. 7, July 1999 (1999-07), pages 795-802, XP002542241 ISSN: 0046-8177
  • DATABASE GENESEQ [Online] 16 January 1991 'Complete sequence of humam TIMP-2 from clone pSS38.' Retrieved from EBI, accession no. GSP:AAR06898 Database accession no. AAR06898
  • DATABASE EMBL [Online] 28 June 2000 'DEPA2042 Rat Lambda ZAP Express Library Rattus norvegicus cDNA 5', mRNA sequence.' Retrieved from EBI, accession no. EM_EST:BE128292 Database accession no. BE128292
  
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).


Description

Field of the Invention



[0001] The present invention relates generally to the field of wound healing and to the repair and maintenance of healthy skin.

Background of the Invention



[0002] One major reason that chronic wounds do not heal is that a class of proteinases destroys the newly formed wound bed (Vaalamo et al., 1997; Weckroth et al., 1996; DiColandrea et al., 1998; Moses et al., 1996). These matrix metalloproteinases (MMPs) are normally prevented from destroying the wound bed by the action of four Tissue Inhibitors of MetalloProteinase (TIMPsl-4) that form very specific inhibitory complexes with the MMPs (e.g., Olson et al., 1997; Taylor et al., 1996; Howard et al., 1991). That is, each TIMP only inhibits a specific subset of MMPs. In chronic wounds the ratio of MMP to TIMP is high, such that most of the MMPs are uninhibited (Vaalamo et al., 1996; Saarialho-Kere, 1998). In fact, with elevated protease levels, the TIMP molecules themselves can be hydrolyzed. There is no naturally occurring TIMP molecule that singly inhibits all types of MMPs.

[0003] Mutated TIMP peptides that can function to inhibit MMPs are known (Butler et al., 1999, Journal of Biological Chemistry, 274, no. 29).

[0004] Hence, further approaches are needed to optimize inhibition of matrix metalloproteinases and to improve wound healing.

Summary of the Invention



[0005] The invention provides polypeptides shorter than 200 amino acids that can inhibit matrix metalloproteinases. The polypeptides provided by the invention are isolated polypeptides shorter than 200 amino acids and comprising SEQ ID NO:5 or SEQ ID NO:20. Also provided are isolated nucleic acids that encode a polypeptide of the invention, that is nucleic acids that encode a polypeptide shorter than 200 amino acids and comprising SEQ ID NO:5 or SEQ ID NO:20. Examples of such isolated nucleic acids include a nucleic acid that comprises SEQ ID NO:6.

[0006] The polypeptides of the invention are useful for treating wounds, including chronic wounds. Hence, the invention provides a composition that comprises a therapeutically effective amount of polypeptide inhibitor shorter than 200 amino acids and comprising SEQ ID NO:5 or SEQ ID NO:20 and a pharmaceutically acceptable carrier. The composition can, for example, be provided in the form of a lotion, gel or cream. Alternatively, the polypeptides can be provided in a wound dressing. Such a wound dressing can include a polypeptide shorter than 200 amino acids and comprising SEQ ID NO:5 or SEQ ID NO:20 and a pharmaceutically acceptable carrier.

[0007] The invention further provides a polypeptide shorter than 200 amino acids and comprising SEQ ID NO:5 or SEQ ID NO:20 for use in treating a wound.

[0008] The polypeptide inhibitors of the invention have many useful properties. For example, these polypeptide inhibitors can promote wound healing, prevent scarring, improve skin tone, or stimulate the development of a smooth, healthy skin. Moreover, they are stable in mammalian serum or plasma.

[0009] The polypeptide inhibitors in the compositions, dressings and methods of the invention can inhibit proteinase activity of any one of matrix metalloproteinase-1, matrix metalloproteinase-2, matrix metalloproteinase-3, matrix metalloproteinase-4, matrix metalloproteinase-5, matrix metalloproteinase-6, matrix metalloproteinase-7, matrix metalloproteinase-8, and matrix metalloproteinase-9, matrix metalloproteinase-10, matrix metalloproteinase-11, matrix metalloproteinase-12, or matrix metalloproteinase-13. In some embodiments, the polypeptide inhibitor can inhibit more than one of these matrix metalloproteinases.

Description of the Figures



[0010] 

Figure 1 provides a photocopy of a 1.5% agarose gel showing DNA from recombinant clones. Ligated gene-expression vector constructs were transformed into JM109, grown on LB plates supplemented with ampicillin. Individual colonies were picked into liquid media and plasmid was purified from these cultures by mini-prep. Lanes 3, 6, and 8 contained DNA with a size corresponding to a plasmid having a SEQ ID NO:6 insert. These plasmids were further characterized by restriction digest (not shown). The plasmid from lane 3 was picked for protein expression analysis.

Figure 2 provides a photocopy of a molecular visualization of a final energy minimized model for the SEQ ID NO:5 polypeptide. Figure 2A provides a solid CPK space-filled model showing the overall three dimensionality of the protein. Note the TIMP-2 like extension (upper left of the molecule) that rises from the matrix metalloproteinase-binding surface. Figure 2B provides the same view as in 2A, only the display illustrates the secondary structural elements of the protein. Beta strand structures that form the central beta barrel motif are shown in light gray, loops and turns are in white, and the single alpha helix is shown in dark gray. The protein is shown as a trace through the alpha carbon positions. Both illustrations were made using Rasmol.

Figure 3 illustrates the purification of the SEQ ID NO:5 polypeptide as assessed by 12% SDS PAGE analysis of the maltose binding protein (MBP)-SEQ ID NO:5 polypeptide fusion and the purified SEQ ID NO:5 polypeptide. The expression and purification of the protein followed the protocol described in Example 1. Lane 1, approximately 5 µg of the MBP-SEQ ID NO:5 polypeptide fusion (Fraction II); Lane 2, approximately 10 µg of purified (Fraction IV) SEQ ID NO:5 polypeptide. The gel was visualized with coomassie stain.

Figure 4 provides a graph summarizing an ELISA analysis of polyclonal antibodies (pAbs) raised against the SEQ ID NO:5 polypeptide. One µg of Fraction IV the SEQ ID NO:5 polypeptide was adsorbed to the wells of a microtiter tray and reacted with either purified pAbs (filled circles) or pre-immune serum (open circles) at the indicated dilution. Visualization was achieved using a goat anti-rabbit secondary antibody that was labeled with Oregon Green-488. A Dynex fluorescent microtiter plate reader was utilized with a 485 nm (excitation) and a 538 nm (emission) bandpass filter set. The fluorescence versus the log of the antibody (or serum) dilution is plotted in this graph.

Figure 5 provides a graph illustrating the enzymatic hydrolysis of fluoresceinated collagen by matrix metalloproteinase-9. The assay measured the release of fluorescein from collagen as a function of time. Substrate was mixed with enzyme at time zero, and collagen destruction was monitored for 1200 seconds (bold line). In a separate reaction an equal amount of the SEQ ID NO:5 polypeptide was added to an ongoing hydrolysis reaction at 200 seconds (the arrow on the graph). The dotted line below indicates that after a short lag period, collagen destruction ceased. Excitation wavelength at 490 nm, emission wavelength at 520 nm.

Figure 6 provides a graph illustrating a titration of matrix metalloproteinase-9 with the SEQ ID NO:5 protein. The fluorescein release assay was used to determine the kinetic parameters of inhibitor function. The indicated stoichiometric amount of the SEQ ID NO:5 polypeptide was added to matrix metalloproteinase-9, and the mixture was incubated at room temperature for 5 minutes. Fluoresceinated collagen and buffer were added to the mixture, and the release of fluorescein was monitored as a function of time. Excitation wavelength at 490 nm, emission wavelength at 520 nm.

Figure 7 provides a bar graph illustrating the inhibitory constants for the SEQ ID NO:5 polypeptide determined for several MMPs. Instantaneous velocity values were extracted from the curves in Figure 6, and were used to calculate Ki values as described in the Procedures section.

Figure 8 provides a photocopy of a molecular visualization of the SEQ ID NO:5 polypeptide. An alpha carbon backbone trace (in light gray) highlights the position of the three-disulfide bonds (shown in dark gray). The two upper disulfide bonds help to maintain the geometry of the MMP binding region, while the lower disulfide bond helps to lock the carboxy terminus into a more rigid conformation. Also shown in light gray is the position of the single tryptophan.

Figure 9 provides a graph illustrating the chemical denaturation of native (filled circles) or reduced (open circles) SEQ ID NO:5 polypeptide. Plotted is the fraction of the protein population that is unfolded as a function of the urea concentration. The SEQ ID NO:5 polypeptide was reduced by incubating the protein with 1mM DTT prior to the addition of urea. Fluorescence emission values were converted into fraction unfolded as described in the Procedures section.

Figure 10 provides a graph illustrating the stability of the SEQ ID NO:5 polypeptide in human serum. One mg of Fraction IV SEQ ID NO:5 polypeptide was added to 1 mL of human serum (closed circles, lower line), 1 mL of PBS (closed circles, upper line), or 0.2 mg of MMP-9 and 1 mL of human serum. The samples were incubated at room temperature. At the times indicated an aliquot was removed from the mixtures and was frozen at -20 °C until the end of the 36-hour period. The aliquots were then analyzed for the SEQ ID NO:5 polypeptide content by ELISA using purified anti SEQ ID NO:5 polypeptide pAbs. Visualization was achieved using a goat anti-rabbit secondary antibody that was labeled with Oregon Green-488. A Dynex fluorescent microtiter plate reader was utilized with a 485 nm (excitation) and a 538 nm (emission) bandpass filter set. Fluorescence was converted to percent SEQ ID NO:5 polypeptide remaining by arbitrarily setting the zero time point to 100%.

Figure 11 provides a graph illustrating the thermal transition of 50 µM SEQ ID NO:5 polypeptide as monitored by intrinsic tryptophan fluorescence. Data were collected and analyzed as described in Example 1. The fraction of the protein population that is unfolded as a function of the temperature is plotted.

Figure 12 provides a thermodynamic characterization of the SEQ ID NO:5 polypeptide. The graph illustrates the thermodynamic stability of the SEQ ID NO:5 polypeptide as a function of temperature as determined by intrinsic tryptophan fluorescence. Included on the plot are free energy values determined at 20 °C and 30 °C by denaturation of the protein in urea (see also Figure 9). Free energy calculations were performed according to the method described in Example 1.

Figure 13 also provides a thermodynamic characterization of the SEQ ID NO:5 polypeptide. The graph is a van't Hoff plot for thermal unfolding of the SEQ ID NO:5 polypeptide monitored by intrinsic tryptophan fluorescence. The natural logarithm of the equilibrium constant versus 1000/T is plotted, where T is the absolute temperature.

Figure 14 provides a graph illustrating an analytical gel filtration analysis of the SEQ ID NO:5 polypeptide. 500 µg of purified the SEQ ID NO:5 polypeptide in PBS was injected onto a BioSelect 125 SEC column and chromatographed in PBS at a flow rate of 0.5 mL/min. The absorbance at 280 nm versus elution time is plotted. Superimposed on the graph are the elution points for myoglobin (12 kDa molecular weight), BSA (65 kDa molecular weight), and the positions of the column void volume (V0) and the total volume (Vt).

Figure 15 provides a model of a predicted molecular complex between matrix metalloproteinase-9 (MMP-9) and the SEQ ID NO:5 polypeptide. The three dimensional coordinate files of MMP-9 (dark gray) and the SEQ ID NO:5 polypeptide (light gray) were used as input into the program FTDOCK (Gabb et al., 1997). The resulting model is the most probable complex that forms between the two proteins. FTDOCK evaluates both geometric and electrostatic considerations when calculating docking interactions. Both terms are combined into a robust Fourier correlation function.

Figure 16 provides a graph illustrating an SPR analysis of MMP-9∼SEQ ID NO:5 polypeptide binding and dissociation. A BiaCore CM-5 chip surface was reacted with MMP-9 through activated carboxyl-amine linkage chemistry. Purified SEQ ID NO:5 polypeptide was flowed over this surface at a rate of 10 µL/min. The binding isotherm shows a high degree of affinity (zero to 400 seconds). At 400 seconds, the flow was replaced with buffer only in order to observe the dissociation phase.

Figure 17 provides a chromatograph illustrating formation of a SEQ ID NO:5 polypeptide∼MMP-9 complex by HPLC analysis. 100 µg of the SEQ ID NO:5 polypeptide was mixed with 700 µg of MMP-9 (approximately 1 mM of each protein) in PBS, and the reaction was incubated at room temperature for 30 minutes in order to effect binding. The material was injected onto a BioSelect 125 SEC column and was chromatographed in PBS at a flow rate of 0.5 mL/min. This trace is marked as "complex" on the figure. In a second reaction, the same amount of the SEQ ID NO:5 polypeptide and MMP-9 were mixed together and were immediately injected onto the SEC column. This trace is marked as "mixture" on the figure.


Detailed Description of the Invention



[0011] The present invention provides inhibitors of matrix metalloproteinases that are useful for promoting wound healing. In general, the present inhibitors and compositions promote wound healing, prevent scarring, improve skin tone and stimulate the development of a smooth, healthy skin.

[0012] According to the invention, a polypeptide with a sufficient degree of amino acid sequence identity to regions of the four Tissue Inhibitors of MetalloProteinase (TIMPs1-4) can form an inhibitory complex with a variety of matrix metalloproteinases. Administration of such a polypeptide inhibits matrix metalloproteinases and diminishes the rate of extracellular matrix destruction in wounds. Hence, such a polypeptide inhibitor can provide a faster rate of wound healing.

[0013] Most inhibition strategies involve preventing enzymatic activity of matrix metalloproteinases with organic small molecules. These compounds are often toxic to the body and are not naturally occurring molecules. Use of natural polypeptides to inhibit matrix metalloproteinases provides a high degree of proteinase control without toxic side effects. Unlike small molecule inhibition strategies, the polypeptides of the invention can be used to inhibit activation of individual or all matrix metalloproteinase classes simultaneously. The polypeptides can be freely introduced onto the skin, into the wound environment, or they can be tethered to, or delivered by, a skin covering or wound dressing.

[0014] The invention provides a high degree of control over the level of proteinase activity for healing chronic wounds. For example, as some amount of proteinase level is required during chronic wound healing (Agren et al., 1999), one of skill in the art may choose to only partially inhibit proteinase activity. By modulating the type and amount of inhibitor polypeptide applied, the degree of matrix metalloproteinase inhibition can be controlled.

Polypeptide Inhibitors



[0015] According to the present invention, polypeptides shorter than 200 amino acids having sequences related to TIMPs are useful for wound healing and for promoting development of healthy skin. As provided herein, the term polypeptide is used synonymously with the term protein. The polypeptides provided by the invention inhibit the activity of many types of matrix metalloproteinases. However, the polypeptide inhibitors are smaller and more stable than naturally occurring TIMP polypeptides. Moreover, the sequence of the present polypeptide inhibitors can be modulated to optimize their binding properties, for example, the polypeptide sequence can be modulated to that it inhibits a broad spectrum of metalloproteinases or the sequence can be changed so that only one or a few metalloproteinases are inhibited.

[0016] For example, a human TIMP-1 can have the following amino acid sequence (SEQ ID NO:1).

See Docherty et al., Sequence of human tissue inhibitor of metalloproteinases and its identity to erythroid-potentiating activity, Nature 318 (6041), 66-69 (1985).

[0017] A human TIMP-2 can have the following amino acid sequence (SEQ ID NO:2).



See Stetler-Stevenson et al., Tissue inhibitor of metalloproteinase (TIMP-2). A new member of the metalloproteinase inhibitor family, Biol. Chem. 264 (29), 17374-17378 (1989).

[0018] A human TIMP-3 can have the following amino acid sequence (SEQ ID NO:3).

See Wick et al., A novel member of human tissue inhibitor of metalloproteinases (TIMP) gene family is regulated during G1 progression, mitogenic stimulation, differentiation, and senescence, J. Biol. Chem. 269 (29), 18953-18960 (1994).

[0019] A human TIMP-4 can have the following amino acid sequence (SEQ ID NO:4).

See Greene et al., Molecular cloning and characterization of human tissue inhibitor of metalloproteinase 4, J. Biol. Chem. 271 (48), 30375-30380 (1996).

[0020] Polypeptide inhibitors of the invention have sequences related to such TIMPs. However, the present polypeptides are shorter and more stable than these TIMPs. In particular, the present polypeptide inhibitors have about 100 fewer amino acids then the naturally available TIMPs. Hence, they are simpler, cheaper and easier to make. More significantly, the present inhibitors have a highly stabilized beta barrel topology that has been enhanced by incorporation of additional cysteine residues. This topology provides an inhibitor that is resistant to denaturation and to protease action.

[0021] The present polypeptide inhibitors can inhibit the activity of many types of matrix metalloproteinases. The present polypeptides can also prevent the activation of proenzyme matrix metalloproteinases, as well as inhibit the enzymatic activity of mature matrix metalloproteinases. For example, polypeptides containing sequences that are more conserved in a variety of TIMPs can be used to provide inhibitors that are generally effective against a variety of matrix metalloproteinases. However, polypeptides containing sequences are less conserved amongst the various TIMPs, for example, sequences unique to a specific TIMP, can be used to provide inhibitors that are specific for individual matrix metalloproteinases.

[0022] The polypeptide inhibitors used in the invention are shorter than two hundred amino acids. Polypeptides shorter than about one hundred fifty amino acids can also be used. Similarly, polypeptides shorter than about one hundred twenty five amino acids are also used in the invention.

[0023] One polypeptide provided by the invention has SEQ ID NO:5, as follows.



[0024] Upon expression in E. coli, the SEQ ID NO:5 polypeptide can be cleaved at its N-terminus so the N-terminal methionine is missing. Such a polypeptide can have SEQ ID NO:20, as follows.



[0025] The SEQ ID NO:5 and SEQ ID NO:20 polypeptide inhibitors show excellent inhibitory properties towards matrix metalloproteinase-9, as well as with other matrix metalloproteinases. The SEQ ID NO:5 and SEQ ID NO:20 polypeptide inhibitors embody several fundamental and desirable properties. First, these proteins are easily purified in a form that is fully folded and soluble. By changing the expression vector, it is possible to produce these proteins in nonbacterial systems, such a bacculovirus, or mammalian cell lines. Second, these proteins are extremely stable and long-lived. This property is related to the beta barrel topology that is maintained and enhanced by addition of cysteine residues that can form stabilizing disulfide bonds. Such stability is an important consideration for a molecule that is to be introduced into a wound environment. Third, the SEQ ID NO:5 and SEQ ID NO:20 polypeptide inhibitors are good, broad range matrix metalloproteinase inhibitors. They form long-lived and stoichiometric complexes with matrix metalloproteinases. Fourth, these SEQ ID NO:5 and SEQ ID NO:20 polypeptide inhibitors are immunogenic so that antibodies can readily be raised against them. These antibodies are useful for tracking the protein(s) during in situ experiments. Fifth, the SEQ ID NO:5 and SEQ ID NO:20 polypeptide inhibitors contain a number of aromatic amino acids (one tryptophan and four tyrosines). Such aromatic amino acids make the SEQ ID NO:5/ SEQ ID NO:20 polypeptide amenable to a host of intrinsic fluorescence experiments, alleviating the need to modify the protein with extrinsic fluorophores.

[0026] Molecular modeling methods were employed in order to design the SEQ ID NO:5 polypeptide inhibitor. The protein was constructed by aligning the amino acid sequences of the four TIMP molecules in order to define regions of high amino acid identity. The SEQ ID NO:5 sequence therefore constitutes a consensus amino acid sequence derived from sequence alignment studies. An analysis of the contact region in the published three-dimensional model of a TIMP-matrix metalloproteinase structure allowed for the removal of a protein domain of approximately 100 amino acids that was not involved in the binding interaction. A disulfide bond was introduced into the synthetic protein inhibitor in order to stabilize the new carboxy terminus.

[0027] The SEQ ID NO:5 and SEQ ID NO:20 polypeptides were produced in good yield in E. coli and were purified to homogeneity (Hodges et al., 1998; Liu et al., 1997). A maltose binding protein fusion purification scheme was employed so that homogeneous SEQ ID NO:5 and SEQ ID NO:20 polypeptides could be prepared from crude extract in a matter of days. However, isolation of the SEQ ID NO:5 and SEQ ID NO:20 polypeptides is not dependent on use of the maltose binding protein fusion scheme. Should it be desired, nucleic acids encoding the SEQ ID NO:5 or SEQ ID NO:20 polypeptide or any other polypeptide of the invention can be cloned into any expression vector that is available.

[0028] A nucleic acid encoding the SEQ ID NO:5 and SEQ ID NO:20 polypeptides was built in approximately three weeks from a series of short oligonucleotides using a combination of hybridization and enzymatic synthesis. The full-length gene sequence was directionally cloned into a protein expression vector and the sequence was verified by DNA sequencing. Design at the nucleotide level aided in cloning experiments by incorporating restriction endonuclease sites into the sequence, and it also helped to maximize protein expression by employing an E. coli codon bias.

[0029] A nucleic acid that encodes the SEQ ID NO:5 and SEQ ID NO:20 polypeptides is, for example, SEQ ID NO:6, provided below.





[0030] To optimize the structural and binding properties of the present polypeptide inhibitors, a full-length amino acid sequence that is an approximate average of the four known TIMP sequences can be generated. This can be done, for example, by performing a robust pair-wise alignment of TIMP amino acid sequences using the program CLUSTAL (Higgins et al., 1992). A consensus sequence was constructed using this type of alignment. For non-conserved amino acids in the contact region, substitutions can be made that preserve the hydrophobic character of the vicinity, but that negate specific side chain-side chain interactions.

[0031] The amino acids involved in binding can be identified and distinguished from those involved in maintaining the stable beta barrel topology. Conservative amino acid substitutions can be made amongst the amino acids that are involved in maintaining the stable beta barrel topology. Less conservative, or even non-conservative, amino acid changes can be made amongst the amino acids involved in binding to metalloproteinases.

[0032] Additional amino acids can be removed or added to the C-terminal domain of the polypeptide inhibitor. Through the analysis of the two TIMP/MMP complex structures, it was apparent that only the N-terminal TIMP region made significant contact with the catalytic domain of the MMP. This was confirmed later by docking the final protein model with MMP-9.

[0033] Such manipulations reduced the overall length of the SEQ ID NO:5 protein from the usual TIMP size of about 225 amino acids to about 108 amino acids. In order to stabilize the new C-terminus of the protein, two additional amino acid replacements were made in the SEQ ID NO:5 and 20 polypeptides: Leu85 and Val101 were changed to cysteine. Structural studies show that these two residues normally were within 3 Å of each other, and could form a disulfide bond if altered to cysteine. In this way the last loop region of the inhibitor polypeptide is locked in place. In addition a cysteine residue in position 13 was changed to serine. Thus all cysteine residues (6) in the SEQ ID NO:5 and 20 polypeptides participate in disulfide bond formation.

[0034] The polypeptide inhibitors of the invention have a beta barrel conformation. As used herein a beta barrel conformation means that the core of the polypeptide comprises beta strand secondary structures that fold into a barrel-like tertiary structure. The beta barrel is stabilized by intra-strand hydrogen bonding and internal hydrophobic packing interactions. A beta barrel is a recognized tertiary structure known to those skilled in the art of protein structure and function.

[0035] In the present invention, the fundamental beta barrel conformation is further stabilized by engineered disulfide bonds that help maintain the overall topology of the folded polypeptide. A polypeptide having SEQ ID NO:5 or SEQ ID NO:20 can fold into a six stranded beta barrel conformation with three disulfide bonds crosslinking the separate beta peptide strands. Amino acids involved in binding matrix metalloproteinases are displayed on the surface of the barrel-like structure.

[0036] The conformation of polypeptides can be determined by any procedure available to one of skill in the art. For example, the conformation can be determined by x-ray crystallography or by computer modeling. For example, computer modeling can be performed using programs such as the Swiss PDB Viewer (Guex and Peitsch, 1997) and Rasmol (Sayle and Milner-White, 1995) programs. Modeling work can be performed on any available computer with sufficient speed and RAM. For example, much of the computer modeling work provided herein was performed on a Compaq PC running Windows 95, as well as a Silicon Graphics, Inc. Octane UNIX workstation. Additionally, the Cerius2 molecular package from Molecular Simulations, Inc. was utilized on the Octane UNIX workstation.

[0037] For comparison, three dimensional structure files of selected matrix metalloproteinases (MMPs) can be downloaded from the Protein Databank as follows (filename, reference): MMP-1 (1FBL, Li et al., 1995), MMP-2 (1GEN, Libson et al., 1995), MMP-8 (1JAO, 1JAN, Grams, et al., 1995; Reinemer et al., 1994), MMP-9 (1MMQ, Browner et al., 1995), TIMP-2/MT-1 MMP complex (1BUV, Fernandez-Catalan et al., 1998), TIMP-2 (1BR9, Tuuttila et al., 1998), and TIMP-1/MMP complex (1UEA, Gomis-Ruth et al., 1997; Huang et al., 1996; Becker et al., 1995). These files can be used to analyze and compare three-dimensional structure of the polypeptide inhibitors with naturally occurring TIMP proteins, and can facilitate identification of the amino acids responsible for specific binding interactions with different matrix metalloproteinases.

[0038] The ability of a polypeptide to inhibit matrix metalloproteinase activity can be assessed by any procedure available to one of skill in the art. Many different assay procedures are available for assessing whether or not an agent can act as an inhibitor of proteinase activity. For example, a protein substrate can be used that generates a detectable signal when cleaved by the proteinase. In some embodiments, the activity of a matrix metalloproteinase in the presence and absence of a test inhibitor is assayed by observing enzymatic hydrolysis of fluoresceinated protein substrate, for example, as a function of time. One example of such a fluoresceinated protein substrate is fluoresceinated collagen available from Molecular Probes, Inc. Such a fluoresceinated protein substrate can be incubated with a selected matrix metalloproteinase, or a mixture of selected matrix metalloproteinases. Cleavage of the fluoresceinated protein substrate is detected by observing an increase in absorbance over time. Varying amounts of substrate and/or test polypeptide inhibitor(s) can be used in the assay mixture to ascertain what concentration effects exist, and what amounts of inhibitor are optimal for inhibiting matrix metalloproteinases.

[0039] The sequence of the present polypeptides can therefore be altered to modulate the affinity of the polypeptide inhibitor for different matrix metalloproteinases. Because some proteinase activity is required (even in chronic wounds) in order to modulate extracellular matrix reorganization (Agren 1999), in certain embodiments it may be desirous to construct an inhibitor that does not inhibit matrix metalloproteinases with an extremely low Ki. For example, the Ki values of the present polypeptides can vary from about 1 µM to about 1 mM. Such a polypeptide would have the ability to allow some transient matrix metalloproteinase activity (due to a relatively high Ki).

[0040] The inhibitory constant (Ki) of a polypeptide inhibitor ([I]) can be determined using procedures provided by Segel (1993) via the use of Dixon plots (1/v vs. [I]), such that:

where Km is the Michaelis constant, Vmax is the reaction maximum velocity, and [S] is the substrate concentration. The degree and the timing of inhibitor activity in the chronic wound can also be controlled by modulating the inhibitor dose and application timing.

[0041] The toxicity of the polypeptide inhibitors of the invention is expected to be low. However, if concerns arise, the cellular toxicity can be assayed by adding various amounts of a polypeptide to fibroblasts or keratinocytes in culture. The growth and cellular integrity of these cells can be monitored to assess whether a selected polypeptide inhibitor has any negative effects.

[0042] The healing rate of a selected polypeptide inhibitor can be assessed by introducing the selected polypeptide into a wound and measuring whether the healing rate is altered by the presence of the polypeptide. For example, the rate of wound healing in the presence and absence of a polypeptide can be determined. While any wound may be used, a wound model with predictable properties is preferred. For example, two animal chronic wound models exist that may be used. The first is an ischemic rabbit ear model, while the second is an induced diabetic rat model.

Wound Healing Compositions



[0043] Polypeptides of the invention can be used to heal wounds and are particularly beneficial for chronic wound healing. Individual polypeptides, polypeptide variants, polypeptide derivatives and mixtures thereof (e.g. those with different sequences) can be combined in a formulation to promote wound healing and to prevent or treat skin problems. Optimal healing and skin regeneration may require some matrix metalloproteinase activity. Hence, the compositions and formulations of the present invention do not necessarily promote maximal inhibition of matrix metalloproteinases. Instead, the activity of the polypeptide inhibitor formulation is varied as needed to optimize healing and promote healthy skin development. Lesser or greater levels of inhibition can be achieved by varying the type, content and amount of inhibitor polypeptides so that healing and healthy skin development is promoted.

[0044] To promote healthy skin development and/or treat wounds, polypeptides of the invention are introduced onto the skin or into wounds in any manner chosen by one of skill in the art. For example, polypeptides can be formulated into a therapeutic composition containing a therapeutically effective amount of one or more polypeptides and a pharmaceutical carrier. Such a composition can be introduced onto skin or into the wound as a cream, spray, foam, gel or in any other form or formulation. In another embodiment, polypeptides of the invention can be formulated into a skin covering or dressing containing a therapeutically effective amount of one or more polypeptides impregnated into, covalently attached or otherwise associated with a covering or dressing material. In one embodiment, the skin covering or dressing permits release of the polypeptide inhibitor. Release of the polypeptide inhibitor can be in an uncontrolled or a controlled manner. Hence, the skin coverings or wound dressings of the invention can provide slow or timed release of the polypeptide inhibitor into a wound. Skin coverings and dressing materials can be any material used in the art including bandage, gauze, sterile wrapping, hydrogel, hydrocolloid and similar materials.

[0045] A therapeutically effective amount of a polypeptide of the invention is an amount of polypeptide that inhibits a matrix metalloproteinase to a degree needed to promote healthy skin development and/or wound healing. For example, when present in a therapeutic or pharmaceutical composition, the amount of polypeptides of the invention can be in the range of about 0.001% to about 35% by weight of the composition. The polypeptides can form about 0.5% to about 20% by weight of the composition. Alternately; the polypeptides form about 1.0% to about 10% by weight of the composition. The therapeutically effective amount of polypeptide inhibitor necessarily varies with the route of administration. For example, a therapeutic amount between 30 to 112,000 µg per kg of body weight can be effective for intravenous administration. However, the amount of the polypeptide inhibitor required for healthy skin development or wound treatment will vary not only with the route of administration, but also the nature of the condition being treated and the age and condition of the patient and will be ultimately at the discretion of the attendant physician or clinician.

[0046] The dosage and method of administration can vary depending upon the location of the skin or tissue to be treated and/or upon severity of the wound. Useful dosages of the polypeptides and polypeptide conjugates can be determined by correlating their in vitro activity, and in vivo activity in animal models described herein. The compound can conveniently be administered in unit dosage form; for example, containing about 0.001 µg to about 10 mg, conveniently about 0.01 µg to about 5 mg, more conveniently, about 0.10 µg to about 1 mg, and even more conveniently about 1.0 µg to 500 µg of polypeptide per unit dosage form. The desired dose may be presented in a single dose, as divided doses, or as a continuous infusion. The desired dose can also be administered at appropriate intervals, for example, as two, three, four or more sub-doses per day. One of skill in the art can readily prepare and administer an effective formulation from available information using the teachings provided herein.

[0047] The polypeptide inhibitors of the invention can be formulated as pharmaceutical compositions and administered to a mammalian host, such as a human patient in a variety of dosage forms adapted to the chosen route of administration, i.e., orally or parenterally, by intravenous, intramuscular, topical or subcutaneous routes.

[0048] Thus, the polypeptide inhibitors may be systemically administered, for example, intravenously or intraperitoneally by infusion or injection. Solutions of the polypeptide inhibitor can be prepared in water, optionally mixed with a nontoxic surfactant. Dispersions can also be prepared in glycerol, liquid polyethylene glycols, triacetin, and mixtures thereof and in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.

[0049] The pharmaceutical dosage forms suitable for injection or infusion or topical application can include sterile aqueous solutions or dispersions or sterile powders comprising the active ingredient that are adapted for the extemporaneous preparation of sterile injectable or infusible solutions or-dispersions, optionally encapsulated in liposomes. In all cases, the ultimate dosage form must be sterile, fluid and stable under the conditions of manufacture and storage. The liquid carrier or vehicle can be a solvent or liquid dispersion medium comprising, for example, water, ethanol, a polyol (for example, glycerol, propylene glycol, liquid polyethylene glycols, and the like), vegetable oils, nontoxic glyceryl esters, and suitable mixtures thereof. The proper fluidity can be maintained, for example, by the formation of liposomes, by the maintenance of the required particle size in the case of dispersions or by the use of surfactants. The prevention of the action of microorganisms can be brought about by various antibacterial and antifungal agents, for example, parabens, chlorobutanol, phenol, sorbic acid, thimerosal, and the like. In some cases, one of skill in the art may choose to include isotonic agents, for example, sugars, buffers or sodium chloride. Prolonged absorption of the injectable compositions can be brought about by the use in the compositions of agents delaying absorption, for example, aluminum monostearate and gelatin.

[0050] Sterile injectable solutions are prepared by incorporating the polypeptide or polypeptide conjugate in the required amount in the appropriate solvent with various of the other ingredients enumerated above, as required, followed by filter sterilization. In the case of sterile powders for the preparation of sterile injectable solutions, methods of preparation include vacuum drying and the freeze-drying techniques, which yield a powder of the active ingredient plus any additional desired ingredient present in the previously sterile-filtered solutions.

[0051] In some instances, the polypeptide inhibitors can also be administered orally, in combination with a pharmaceutically acceptable vehicle such as an inert diluent or an assimilable edible carrier. They may be enclosed in hard or soft shell gelatin capsules, may be compressed into tablets, or may be incorporated directly with the food of the patient's diet. For oral therapeutic administration, the polypeptide inhibitor may be combined with one or more excipients and used in the form of ingestible tablets, buccal tablets, troches, capsules, elixirs, suspensions, syrups, wafers, and the like. Such compositions and preparations should contain at least 0.1% by weight of active compound. The percentage of the compositions and preparations may, of course, be varied and may conveniently be between about 2 to about 60% of the weight of a given unit dosage form. The amount of active compound in such therapeutically useful compositions is such that an effective dosage level will be obtained.

[0052] The tablets, troches, pills, capsules, and the like may also contain the following: binders such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch, alginic acid and the like; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, fructose, lactose or aspartame or a flavoring agent such as peppermint, oil of wintergreen, or cherry flavoring may be added. When the unit dosage form is a capsule, it may contain, in addition to materials of the above type; a liquid carrier, such as a vegetable oil or a polyethylene glycol. Various other materials may be present as coatings or to otherwise modify the physical form of the solid unit dosage form. For instance, tablets, pills, or capsules may be coated with gelatin, wax, shellac or sugar and the like. A syrup or elixir may contain the active compound, sucrose or fructose as a sweetening agent, methyl and propylparabens as preservatives, a dye and flavoring such as cherry or orange flavor. Of course, any material used in preparing any unit dosage form should be pharmaceutically acceptable and substantially non-toxic in the amounts employed. In addition, the polypeptide inhibitor may be incorporated into sustained-release preparations and devices.

[0053] Useful solid carriers include finely divided solids such as talc, clay, microcrystalline cellulose, silica, alumina and the like. Useful liquid carriers include water, alcohols or glycols or water-alcohol/glycol blends, in which the present compounds can be dissolved or dispersed at effective levels, optionally with the aid of non-toxic surfactants. Adjuvants such as fragrances and additional antimicrobial agents can be added to optimize the properties for a given use.

[0054] Thickeners such as synthetic polymers, fatty acids, fatty acid salts and esters, fatty alcohols, modified celluloses or modified mineral materials can also be employed with liquid carriers to form spreadable pastes, gels, ointments, soaps, and the like, for application directly to the skin of the user.

[0055] In general, the polypeptides of the invention are administered topically for wound treatment and for promoting healthy skin development. The active polypeptides may be administered topically by any means either directly or indirectly to the selected tissue as sprays, foams, powders, creams, jellies, pastes, suppositories or solutions. The term paste used in this document should be taken to include creams and other viscous spreadable compositions such as are often applied directly to the skin or spread onto a bandage or dressing. Polypeptides of the invention can be covalently attached, stably adsorbed or otherwise applied to a skin covering or wound dressing material. To facilitate healing after surgery, the active polypeptides of the invention can be applied directly to target tissues or to prosthetic devices or implantable sustained released devices. The compositions can be administered by aerosol, as a foam or as a mist, with or without other agents, directly onto the skin or wound.

[0056] The polypeptides can be administered in a formulation that can include an emulsion of the polypeptide in a wax, oil, an emulsifier, water, and/or a substantially water-insoluble material that forms a gel in the presence of water. The formulation provides the desirable properties of an emulsion, in that it is spreadable and has the creamy consistency of an emulsion, yet that does not break down when subjected to normal sterilization procedures, e.g. steam sterilization, because the gel stabilizes the emulsion. It also exhibits better water retention properties than a conventional gel because water is held both in the emulsion and in the gel.

[0057] The formulation can also contain a humectant to reduce the partial vapor pressure of the water in the cream or lotion to reduce the rate at which the cream or lotion dries out. Suitable humectants are miscible with water to a large extent and are generally suitable for application to the skin. Polyols are especially suitable for the purpose and suitable polyols may include monopropylene glycol or glycerin (glycerol). The polyol may be present in proportions of 20-50% (by weight) of the total formulation; alternatively the range is 30-40%. This relatively high proportion of polyol also ensures that if the paste should dry out to any degree, the resulting paste remains soft and flexible because the glycerin may act as a plasticiser for the polymer. When the paste is applied on a bandage, for example, it may therefore still be removed easily from the skin when the paste has lost water without the need to cut the bandage off. The polyol also has the advantage of functioning to prevent the proliferation of bacteria in the paste when it is in contact with the skin or wound, particularly infected wounds.

[0058] The formulation can include other ingredients such as antibacterial agents, antifungal agents, anti-inflammatory agents, and the like. Other ingredients may also be found suitable for incorporation into the formulation.

[0059] An example of a wax for the emulsion is glyceryl monostearate, or a combination of glyceryl monostearate and PEG100 stearate that is available commercially as CITHROL GMS/AS/NA from Croda Universal Ltd. This combination provides both a wax and an emulsifier (PEG 100 stearate) that is especially compatible with the wax, for forming an emulsion in water. A second emulsifier can be included in the formulation to increase the stability of the emulsion, for example, a PEG20 stearate, such as CITHROL-1OMS that is supplied by Croda Universal Ltd. The total concentration of emulsifier in the cream should normally be in the range of from 3-15%. Where two emulsifiers are used, one may be present in a greater concentration than the other.

[0060] The water-insoluble material forms a gel with the water of the formulation. The material is therefore hydrophilic but does not dissolve in water to any great extent. The material can be a polymeric material, for example, a water-absorbing non water-soluble polymer. However, non-polymeric materials that form gels with water and that are stable at elevated temperatures could also be used, e.g. clays such as kaolin or bentonite. Some polymers used in the invention are super-absorbent polymers such as those disclosed in WO-92/16245 and that comprise hydrophilic cellulose derivatives that have been partially cross-linked to form a three dimensional structure. Suitable cross-linked cellulose derivatives include those of the hydroxy lower alkyl celluloses, wherein the alkyl group contains from 1 to 6 carbon atoms, e.g. hydroxyethyl cellulose or hydroxypropylcellulose, or the carboxy-celluloses e.g. carboxymethyl hydroxyethyl cellulose or carboxy methylcellulose. An example of a polymer that may be used in the invention is a partially cross-linked sodium carboxy methylcellulose polymer supplied as AKUCELL X181by Akzo Chemicals B.V. This polymer is a superabsorbent polymer in that it may absorb at least ten times its own weight of water. The cross-linked structure of the polymer prevents it from dissolving in water but water is easily absorbed into and held within the three-dimensional structure of the polymer to form a gel. Water is lost less rapidly from such a gel than from a solution and this is advantageous in slowing or preventing the drying out of the cream formulation. The polymer content of the formulation is normally less than 10%, for example, the polymer content can range from about 0.5 to about 5.0% by weight, or from about 1.0% to about 2% by weight.

[0061] The formulation may be sterilized and components of the formulation should be selected, by varying the polymer content, to provide the desired flow properties of the finished product. That is, if the product to be sterilized, then the formulation should be chosen to give a product of relatively high viscosity/elasticity before sterilization. If certain components of the formulation are not to be sterilized, the formulation can be sterilized before addition of those components, or each component can be sterilized separately. The formulation can then be made by mixing each of the sterilized ingredients under sterile conditions. When components are separately sterilized and then mixed together, the polymer content can be adjusted to give a product having the desired flow properties of the finished product. The emulsion content determines the handling properties and feel of the formulation, higher emulsion content leading to increased spreadability and creaminess.

[0062] The formulation may be packaged into tubes, tubs or other suitable forms of containers for storage or it may be spread onto a substrate and then subsequently packaged. Suitable substrates include dressings, including film dressings, and bandages.

[0063] The following examples are intended to illustrate but not limit the invention.

EXAMPLE 1: Procedures



[0064] This Example provides the materials and methods employed for various experiments.

[0065] Molecular Biology Procedures: Bacterial growth conditions and culturing were performed as described by Miller (1972). Unless otherwise noted all procedures performed in this study were according to Maniatis et al. (1982) or Sambrook et al. (1989) or Sambrook et al. (2001); including, agarose gel electrophoresis, and restriction endonuclease digestions. Vent DNA polymerase used in all PCR reactions was purchased from New England Biolabs and was used with the supplied buffer. DNA sequencing (Sanger et al., 1977) was performed using an Applied Biosystems, Inc. automated sequencer, and was performed by Genosys, Inc. DNA oligonucleotides were synthesized by Genosys, Inc. Protein concentration was determined according to the method of Bradford (1976) using bovine serum albumin (BSA) as a standard or spectrophotometrically using a calculated molar absorption coefficient of 11,300 M-1 cm-1. Analytical gel filtration experiments were performed according to Siegel and Monty (1966) using a 7 x 250 mm BioSelect SEC-125 column from BioRad, Inc. All bacterial strains were purchased from the New England Biolabs, Inc. Protein SDS PAGE gels were made, run, and processed as per Laemmli (1970). Chemical reagents and chromatography resins were from Sigma Chemical Co. (St. Louis, MO), except where specifically noted.

[0066] Molecular modeling: Molecular modeling utilized two visualization programs, Swiss PDB Viewer (Guex and Peitsch, 1997) and Rasmol (Sayle and Milner-White, 1995). Model work was performed on a Compaq PC running Windows 95, as well as a Silicon Graphics, Inc. Octane UNIX workstation. Additionally, the Cerius2 molecular package from Molecular Simulations, Inc. was utilized on the Octane. Three dimensional structure files of selected matrix metalloproteinases (MMPs) were downloaded from the Protein Databank as follows (filename, reference): MMP-1 (1FBL, Li et al., 1995), MMP-2 (1GEN, Libson et al., 1995), MMP-8 (1JAO, 1JAN, Grams, et al., 1995; Reinemer et al., 1994), MMP-9 (1MMQ, Browner et al., 1995), TIMP-2/MT-1 MMP complex (1BUV, Fernandez-Catalan et al., 1998), TIMP-2 (1BR9, Tuuttila et al., 1998), and TIMP-1/MMP complex (1UEA, Gomis-Ruth et al., 1997; Huang et al., 1996; Becker et al., 1995). These files were used to analyze the three-dimensional structure of the proteins, the chemical nature of amino acids at various positions and the identification of conserved and variant amino acids in the MMP-TIMP contact interface. This information was utilized to design the inhibitors of the invention that would bind many matrix metalloproteinase enzymes.

[0067] The first step was to begin with a full-length amino acid sequence that was an average of the four known TIMP sequences. A robust pair wise alignment of the four TIMP amino acid sequences was calculated using the program CLUSTAL (Higgins et al., 1992). A consensus sequence was then constructed based on this alignment. For non-conserved amino acids in the contact region, substitutions were made that preserved the hydrophobic character of the vicinity, but that negated specific sidechain-sidechain interactions. Through this exercise, a consensus binding interface was obtained. The large flexible loop portion of TIMP-2, that is not evident in TIMP-1, was built back into the polypeptide inhibitor with several amino acid sequence changes.

[0068] The second step was to remove the C-terminal domain of the consensus inhibitor molecule. Through the analysis of the two TIMP/MMP complex structures, it was determined that only the N-terminal TIMP region made significant contact with the catalytic domain of the MMP. This was confirmed later by docking the final protein model with MMP-9. This manipulation also reduced the overall length of the protein from 225 amino acids to 108 amino acids. In order to stabilize the new C-terminus of the protein, two additional amino acid replacements were made: Leu85 and Val101 were changed to cysteine. These two residues were observed to be within 3 Å of each other. Hence, substitution with cysteine would likely permit formation of a disulfide bond. In this way the last loop region of the protein would be locked in place. In addition a cysteine residue in position 13 was changed to serine. Thus six cysteine residues were available in the final protein inhibitor to participate in disulfide bond formation.

[0069] The third step entailed building a homology model of the new protein inhibitor. The final 108 amino acid sequence of the inhibitor was threaded onto the alpha carbon trace of TIMP-2 using the programs ProMod and SwissModel (Peitsch, 1996; Peitsch et al., 1996). This model was then subjected to energy minimization using a GROMOS 96 forcefield, and several rounds of molecular mechanics geometry optimization using the SYBYL forcefield (Clark et al., 1989). The final minimized/optimized model was then analyzed for bad side chain interactions and torsional geometry.

[0070] The finalized polypeptide inhibitor derived from such three-dimensional modeling had SEQ ID NO:5 and was designated DST. This acronym is short for Delta (the final protein has the C-terminal TIMP domain deleted) Synthetic (it is based on structural and homology modeling) TIMP (because it is based on TIMP1-2 structures).

[0071] Gene design, construction, and-cloning: The final SEQ ID NO:5 amino acid sequence was back translated using the standard genetic code. Codon choice was based on E. coli codon bias, meaning that the final codon selected for a particular amino acid was the most frequently used codon for that amino acid in E. coli. The full-length structural gene was 327 bp. In order to build the gene sequence, ten single-stranded oligonucleotides that spanned the coding region were synthesized by Genosys, Inc. The oligonucleotides were 70 nucleotides in length. Each oligonucleotide was complementary to another oligonucleotide, such that when hybridized with its binding partner, the resulting fragment contained a central duplex region of 50 base pairs and was flanked on each end by a 10 nucleotide single-stranded region. The oligonucleotide sequences employed are shown in Table 3.





[0072] The construction of the inhibitor nucleic acid (SEQ ID NO:6) was done in three separate steps.

[0073] First, 5 µg of each oligonucleotide and its complementary binding partner (for five separate reactions) were mixed together in 10 mM Tris-HCl (pH 7.2), 10 mM NaCl in a final volume of 10 µL. The specific oligonucleotide used in the hybridization mixtures were (see Table 3): (1 and 6), (2 and 7), (3 and 8), (4 and 9), and (5 and 10). The mixture was heated in a water bath at 95 °C for 10 minutes. The heat was turned off, and the entire water bath was allowed to cool to room temperature over a period of five hours.

[0074] Second, aliquots (10 µL) from each of the five "slow cool" reactions were mixed together (final volume 50 µL). The tube was heated at 45 °C for 10 minutes and then was placed into an ice bath. T4 DNA ligase and buffer (New England Biolabs) were added to the tube, and the reaction (final volume 60 µL) was incubated at 16 °C for 20 hours.

[0075] Third, the full-length nucleic acid having SEQ ID NO:6 was selected from the mixture of fragments using two PCR primers (Table 3,11 and 12) that were complimentary to the extreme 5' and 3' ends of the structural gene. This step ensured that only full-length nucleic acids would be amplified. In addition the 3' amplification primer contained a Hind III site to facilitate cloning. The PCR reaction was performed using 1 µL of the ligation mixture described in the foregoing paragraph. The PCR conditions employed were as follows: 95 °C, 1 minute; 49 °C, 1 minute; 72 °C, 30 seconds. Thirty cycles of this program were performed in a Techne Progene PCR device. A ten minute 72 °C extension incubation was performed after the last PCR cycle. The PCR reaction product was verified by DNA agarose gel electrophoresis.

[0076] The PCR reaction product was purified via a Promega DNA Wizard PCR clean-up kit. Prior to cloning, the DNA fragment was treated with T4 DNA polymerase in the presence of ATP in order to ensure fully duplex ends. This reaction was performed according to the instructions from New England Biolabs, Inc. The DNA was re-purified using the Promega DNA Wizard PCR clean-up kit. Then the DNA was digested with Hind III and was purified by ethanol precipitation. The final DNA was resuspended in a small volume of 10 mM Tris-HCl (pH 8.0), 1 mM EDTA.

[0077] The cloning vector, pMAL-c2 (New England Biolabs), was digested with Xmn I and Hind III, and was purified using the Promega DNA clean-up kit. This digest produced a linear vector that contained a 3' blunt end and a 5' Hind III end that was compatible with the 5' blunt end and the 3' Hind III end of the DNA fragment. This combination ensured directional, in-frame cloning of the SEQ ID NO:6 DNA fragment. The vector and the SEQ ID NO:6 DNA fragment were mixed in approximately 1:10 molar ratio and were ligated together in the presence of T4 DNA ligase at 16 °C for 20 hours (total reaction volume was 20 µL). Competent JM109 bacteria were transformed with 5 µL of the ligation reaction. After growth on LB with 60 µg/mL ampicillin agar plates, single colonies were selected, and plasmid was purified from the colonies by the miniprep procedure using a Promega miniprep DNA isolation kit. Isolated plasmids were evaluated by DNA agarose gel electrophoresis, restriction endonuclease digestion, and finally by DNA sequencing. The plasmid construct that encoded the SEQ ID NO:5 polypeptide was designated pDSTe.

[0078] Purification of the protein inhibitor: The expression strategy utilized the T4 RNA polymerase over-expression system from New England Biolabs, Inc. The vector used for protein expression was pMAL, which contains the gene sequence for the maltose binding protein upstream of a multiple cloning site. The SEQ ID NO:6 nucleic acid was inserted into this multiple cloning site. A 1% innoculum of TB-1 cells containing the SEQ ID NO:6 expression vector were grown at 37 °C in Luria broth supplemented with 1% glucose and 60 µg/mL ampicillin. IPTG was added to a final concentration of 0.5 mM when the cells had reached an A595 value of 0.8 (at approximately three hours post-inoculation). Cell growth continued for five additional hours before harvesting. Typically, 5 g of cells was obtained per liter.

[0079] Cells were pelleted by centrifugation at 10,000 x g for ten minutes and resuspended in one volume of 10 mM Tris-HCl, pH 8.0. The cells were respun as above and were frozen for at least 2 hours at -70 °C. The frozen pellet was resuspended in two volumes of BPER E. coli protein extraction buffer. The mixture was incubated at 30 °C for 20 minutes with occasional mixing. The resulting extract was clarified by centrifugation at 12,000 x g for 20 minutes, and the supernatant was dialyzed against 20 mM Tris-HCl (pH 7.4), 200 mM NaCl, 1 mM EDTA (Buffer I). The dialyzed material was diluted to a final concentration of 2.5 mg/mL with Buffer I, and was designated as Fraction I. All subsequent chromatography steps were performed at room temperature.

[0080] Fraction I was applied to a 10 cm x 7.6 cc2 amylose resin column that had previously been equilibrated with Buffer I. The column was then washed extensively with Buffer I (usually 10 column volumes) to remove unbound material. The bound fusion protein was eluted from the column by the application of Buffer I, 10 mM maltose. A typical elution volume was about 2 column volumes. Fractions were assayed for protein content spectrophotometrically, and protein-containing fractions were pooled. This material was designated as Fraction II. Protein concentration was adjusted to 1 mg/mL via Centricon (Amicon, Inc.).

[0081] Fraction II was mixed with Factor Xa protease at a weight/weight stoichiometry of 100:1 (typical reactions contained 50 mg of fusion protein and 0.5 mg of Factor Xa). Cleavage reactions proceeded at room temperature for 24 hours. The extent of cleavage was monitored by SDS PAGE analysis of aliquots removed at various time points during the reaction. The final mixture was dialyzed versus 20 mM Tris-HCl (pH8.0), 25 mM NaCl, 3 mM EDTA and was designated as Fraction III.

[0082] Fraction III was applied to a Mono Q ion exchange column (6 cm x 7.6 cc2) that had been equilibrated in 10 mM Tris-HCl (pH 8.0), 25 mM NaCl (Buffer II). The column was run as follows: Buffer II, 30 mLs; Buffer II with a linear gradient from 25 mM to 500 mM NaCl, 40mLs. Maltose binding protein eluted from the column in 125 mM NaCl, the homogeneous protein inhibitor eluted in 250 mM NaCl, and the Factor Xa protease eluted in 400 mM NaCl. Fractions containing the protein inhibitor were pooled. The material was dialyzed against Buffer II, concentrated to 10 mg/mL, and was designated as Fraction IV. The protein was stored in aliquots at -20 °C. All subsequent experiments were performed with Fraction IV protein, unless specifically noted. The purified SEQ ID NO:5 protein was designated DST.

[0083] Inhibition of MMPs: The assay employed measured the enzymatic hydrolysis of fluoresceinated collagen by MMP-9 or other matrix metalloproteinases as a function of time. Fluoresceinated collagen at a concentration of 5 µM was added to reaction buffer (50 mM Tris- HCl (pH 7.6), 150 mM NaCl, 5 mM CaCl2, 0.1 mM NaN3) and was placed into a Spectrosil quartz fluorimeter cuvette. MMP at a concentration of 0.1 µM was mixed with varying amounts of polypeptide inhibitor (SEQ ID NO:5 or SEQ ID NO:20) and incubated at 25 °C for 10 minutes in order to effect binding. The protein mixture was added to the collagen substrate, and mixed. Fluorescence emission intensity at 520 nm was measured as a function of time using an excitation wavelength of 495 nm in a Shimadzu RF5301 fluorimeter. The fluorescein release assay was used to determine the inhibitory constant (Ki) of the protein based matrix metalloproteinase inhibitor ([I]) according to Segel (1993) by using Dixon plots (1/v vs. [I]), where:

where Km is the Michaelis constant, Vmax is the reaction maximum velocity, and [S] is the substrate concentration.

[0084] Production of polyclonal antibodies: Polyclonal anti-sera was produced by Genosys, Inc. Polyclonal antibodies (pAb) directed against the SEQ ID NO:5 polypeptide were induced by subcutaneous injection of homogeneous SEQ ID NO:5 polypeptide (300 µg) in a 1:1 homogenate with Freund's complete adjuvant into female New Zealand White rabbits. Three subsequent injections of antigen (200 µg) with incomplete adjuvant were performed at weekly intervals. One week after the last injection, the rabbits were bled via an ear cannula. The cleared plasma was collected by centrifugation at 14,000 x g and stored at -20 °C until use.

Purification of polyclonal antibodies:



[0085] The pAbs were purified to homogeneity by affinity chromatography on DEAE Affigel Blue. A rabbit polyclonal antibody isolation kit from BioRad Labs, Inc. was employed according to the supplied instructions, with several minor modifications. The protocol is as follows: The cleared rabbit serum (5 mLs) was passed over an Econo-Pac 10DG desalting column. The pAbs were eluted from the column using the supplied running buffer (0.02 M Tris HCl (pH 8.0), 0.028 M NaCl), and were collected as a single fraction. At this stage the protein concentration was determined using the Bradford assay. The entire serum sample (usually 25 mLs) was passed over the column in 5 mL batches. Between batches the column was washed with 40 mL of running buffer (two column volumes). The final desalted samples from the individual column runs were pooled. This pooled sample was applied to the DEAE Affi-gel Blue column as a single load, the column was washed with 5 column volumes of running buffer (50 mLs), and the pAb fraction was eluted from the column by the application of 5 column volumes of elution buffer (0.025 M Tris HCl (pH 8.0), 0.025 M NaCl). The eluted material was collected as 5 mL fractions. The purity of the IgG fraction was estimated by SDS PAGE. Appropriate fractions were pooled, concentrated to 2 mg/mL by pressure filtration, and were stored at -70 °C until needed. The DEAE Affi-gel Blue column was regenerated by washing the column with 2 M NaCl, 1.5 M sodium thiocyanate in running buffer (10 column volumes), followed by re-equilibration in running buffer. The flow rate for all chromatography steps was maintained at 1.0 mL/min.

[0086] ELISA analysis: ELISAs were performed using methods described by Kaiser and Pollard, (1993) or by Quirk et al. (1996). One µg of purified SEQ ID NO:5 or SEQ ID NO:20 polypeptide was adsorbed to the surface of a 96- well microtiter plate (Immulon 2, Dynatech Labs). The wells were blocked with phosphate buffered saline (PBS) supplemented with 10% BSA. Polyclonal antibodies in blocking buffer were added at various dilutions and were allowed to react with the bound polypeptide inhibitor at room temperature for one hour. Following three washes in PBS, visualization was achieved via a goat anti-rabbit secondary antibody that is conjugated with horseradish peroxidase (Santa Cruz Biotechnology, Inc.). The secondary antibody was added at a 1:2000 dilution in blocking buffer and was incubated at room temperature for one hour. After three washes in PBS, color development is achieved by adding a solution containing 50 mM sodium citrate, 50 mM citric acid, 1 mg/mL o-phenylenediamine, and 0.006% H2O2. After suitable color development (typically 5 to 10 minutes of incubation at room temperature) 50 µL of 2 M sulfuric acid was added to stop the reaction and stabilize the product. Absorbance was measured at 490 nm using an automatic ELISA plate reader (Molecular Dynamics, Inc.). Alternatively, fluoresceinated goat anti-rabbit secondary antibody (Molecular Probes, Inc.) was utilized for the ELISA. For these assays, a Dynex, Inc. fluorescent microtiter plate reader was employed with a 485 nm (excitation) and a 510 nm (emission) bandpass filter set.

[0087] Intrinsic tryptophan fluorescence: Chemical denaturation Stability measurements of the protein inhibitor were performed by measuring protein unfolding in the presence of urea via intrinsic tryptophan fluorescence (Lakowicz, 1983) in a Shimadzu RF5301 fluorimeter. The excitation and emission wavelengths were 295 nm and 340 nm respectively. Both excitation and emission monochrometer slits were set at 1.5 nm. Protein (20 µM) was mixed with increasing amounts of urea (in the concentration range of zero to 6.8 M), and the samples were incubated at room temperature for ten hours to ensure that unfolding equilibrium had been achieved. Relative fluorescence was converted into free energy values according to the relation (Pace et al., 1989):

where yf and yu are the relative fluorescence values for fully folded and fully unfolded SEQ ID NO:5 polypeptide respectively, yi is the relative fluorescence of the unfolding intermediates, T is the absolute temperature, and R is the gas constant. Linear regression and extrapolation of the relationship ΔG versus [urea] was employed to determine the free energy value in the absence of denaturant (ΔGH2O). Similarly, the fraction unfolded protein (Fu) was calculated from the fluorescence data according to the relation (Pace et al., 1989):


Thermal denaturation.



[0088] The intrinsic tryptophan fluorescence of homogeneous SEQ ID NO:5 polypeptide in 25 mM Tris- HCl (pH 8.5), 50 mM Nad was measured in a Shimadzu R5301 fluorimeter (excitation wavelength 295 nm, emission wavelength 340 nm). Temperature was controlled via a stirred water-jacketed sealed quartz fluorimeter cuvette connected to a digital water bath that was accurate to +/- 0.1 °C. Dry nitrogen gas was flushed through the sample compartment continuously to control condensation. Temperature changes were made at a rate of 0.2 °C per minute. The sample was allowed to incubate at temperature for five minutes prior to reading the fluorescence in order to ensure that the system had come to thermal equilibrium. The fluorescence values determined from the thermal experiments were normalized using equation (3) above. The calculated Fu values were converted into the equilibrium constant (KD) using the following equation (4):

By setting In KD = 0, the following van't Hoff equation (5) can be utilized to calculate the values of the transition temperature (Tm) and the corresponding enthalpy at the transition temperature (ΔHm) (Arnold and Ulbrich-Hofmann, 1997):

If ΔG is set to zero in the Gibbs equation, then the entropy at the transition temperature (ΔSm) can be calculated as follows:

Free energy values for the transition temperature region were calculated from the following equation:

These free energy values were substituted into the Gibbs-Helmholtz equation (8) in order to compute the heat capacity.

Finally, the temperature of maximum stability (Tmax) was calculated according to the following equation (9):



[0089] Surface Plasmon Resonance: The BiaCore, Inc. BiaCore-X surface plasmon resonance (SPR) device was utilized to measure the interaction between SEQ ID NO:5 polypeptide (also called the DST protein) and matrix metalloproteinase-9 (MMP-9). For these experiments a carboxymethyl dextran sensor chip (CM-5, Lofas et al., 1993) was activated with 50 mM N-hydroxysuccinimide, 0.2 M N-ethyl-N'-(dimethylaminopropyl)-carbodiimide at a flow rate of 10 µL per minute for ten minutes. SEQ ID NO:5 polypeptide at a concentration of 75 ng/µL was coupled to the activated surface at a flow rate of 10 µL per minute for ten minutes. The final surface was inactivated by flowing 1 M ethanolamine-HCl at a rate of 10 µL per minute for five minutes over the sensor surface. MMP-9 was flowed over the sensor surface at a rate of 20 µL per minute, and at concentrations that ranged from 1 to 100 nM. Binding isotherms were evaluated by simultaneously fitting the forward (ka) and reverse (kd) rate constants to:

(Karlsson and Falt, 1997) where [DST], [MMP-9], and [DST~MMP-9] are the concentrations of free SEQ ID NO:5 polypeptide (DST), free MMP-9, and the complex respectively. The equilibrium affinity constant (KA) is then defined as:

Equation 10 is properly expressed in terms of the SPR signal (Morton et al., 1995) as:

where R is the SPR signal (in response units, RU) at time t, Rmax is the maximum MMP-9 binding capacity in RU, and C is the SEQ ID NO:5 polypeptide concentration. Kinetic analysis (O'Shannessy et al., 1993) was performed using Origin from Microcal, Inc.

EXAMPLE 2: Polypeptide Inhibitor Properties


Molecular Cloning



[0090] Molecular visualization analysis of matrix metalloproteinase (MMP) and MMP∼TIMP three dimensional structures provided structural information for design of the SEQ ID NO:5 polypeptide. The final amino acid sequence of the SEQ ID NO:5 protein can bind a variety of matrix metalloproteinase molecules. The SEQ ID NO:6 nucleic acid that encodes the SEQ ID NO:5 polypeptide employs the codon bias of E. coli in order to maximize expression.

[0091] Construction of the 327 nucleotide SEQ IDNO:6 sequence required a series of short oligonucleotides, because it is currently very difficult to construct nucleic acids that are over 100 bases in length. In addition, it is difficult to efficiently hybridize longer nucleic acid molecules. Hence construction was carried out using a series of hybridization steps. When mixed together in equimolar amounts, the individual oligonucleotides (SEQ ID NO:8-19) were efficiently converted into duplex molecules by a "slow cool" hybridization step. Slowly reducing the temperature from 95 °C over a period of hours favored the formation of short duplexes.

[0092] The resulting fragments contained a central double stranded region of 50 to 60 base pairs that were flanked by 10 nucleotide single-stranded termini. These "sticky ends" were used to drive the assembly of the full-length nucleic acid, again by hybridization. The full-length nucleic acid was formed by heating an equimolar mixture of the duplex molecules at 45 °C for 10 minutes. This step disrupted any partially formed duplex structures formed by association of the termini, but would not disrupt the fully formed central duplex regions. The heated material was "quick cooled" by placing the reaction tube on ice. This hybridization step favored the hybridization of short regions of DNA (i.e.- the 10 base sticky ends). Closure of the phosphodiester backbone of the 327 bp DNA fragment was performed by use of the enzyme T4 DNA ligase.

[0093] The full-length nucleic acid was selected from the resulting mixture of fragments by PCR amplification. This step was far more efficient than purifying the full-length nucleic acid from agarose gels. This step also resulted in a large amount of material for subsequent cloning steps. The ends of the SEQ ID NO:6 nucleic acid were prepared for cloning by making one end blunt using T4 DNA polymerase and using Hind III on the other end to generate a Hind III-compatible end. This resulted in a DNA molecule that could be efficiently and directionally cloned into protein expression vectors.

[0094] Figure 1 shows the result of this cloning. Three out of nine examined colonies contained vector with the correct insert (SEQ ID NO:6). The validity of the insert was confirmed by DNA sequencing; several clones had a sequence corresponding to SEQ ID NO:6.

Physical Properties of the SEQ ID NO:5 Polypeptide



[0095] The SEQ ID NO:5 polypeptide protein is 108 amino acids in length and has a total molecular weight of 108 kDa. A three dimensional model of SEQ ID NO:5 polypeptide was prepared by threading the SEQ ID NO:5 sequence onto the three dimensional alpha carbon backbone of TIMP-2 using the program SwissModel. The optimal thread result was converted into a three dimensional structure that included amino acid side chain positions using the program ProMod. This initial model was subjected to a round of simulated annealing in order to minimize side chain clashes. Several rounds of a SYBYL level geometry optimization put all dihedral angles and torsions into proper geometry. A final round of energy minimization using a GROMOS96 parameter set, without a reaction field was employed. These results are shown in Table 4. The final model has an overall energy of -3534 kJ/mol and is shown in Figure 2. All amino acid residues are within allowable. Ramachandran space (data not shown) and there are no steric clashes.
Table 4: GROMOS 96 energy minimization results for the homology model (only the major parameters from the forcefield are shown).
ParameterEnergy (kJ/mol)
Bonds 66
Angles 541
Torsions 667
Impropers 103
Nonbonded -3027
Electrostatic -1884
Constraints 0
Total: -3534


[0096] Several properties of the SEQ ID NO:5 polypeptide are shown in Table 5.
Table 5: Miscellaneous properties of the SEQ ID NO:5 polypeptide.
Length (amino acids): 108
Molecular weight: 11.8
Isoelectric point: 6.5
Hydrophobic (%): 39.8
Hydrophilic (%): 33.3
Basic (%): 13.9
Acidic (%): 13.0
Stokes radius (Å): 22
Frictional coefficient: 1.2
Alpha helix (%): 9
Beta Strand (%): 62
Loop/coil (%): 29
Tryptophan (#): 1
Tyrosine (#): 4


[0097] The single designed-in tryptophan greatly aided in intrinsic fluorescence experiments (see below). The protein was designed as a single polypeptide that forms a six-stranded beta barrel (Figure 2). The top region of the molecule forms a molecularly flat structure that is held together in part by the formation of two disulfide bonds, between residues Cys2-Cys73 and Cys4-Cys102. (Figure 2). This region forms the basis of the binding domain. This area is flanked by a TIMP-2 like arm formed by a flexible loop region spanning residues Ser31 to Lys41. The loop is stabilized to the main structure by a series of hydrogen bonds. The flexible loop may act as a TIMP recognition domain, and molecular dynamics simulations (data not shown) indicate that it is highly mobile, with deflections that exceed 4 Å. The molecular dimensions of the SEQ ID NO:5 polypeptide are approximately 21 x 18 x 25 Å (total molecular volume of 9455 Å3, total solvent accessible surface area of 9867 Å2).

Expression in E. coli



[0098] Amino acid sequencing of the amino terminal end of purified SEQ ID NO:5 polypeptide revealed that the N-terminal methionine is removed in E. coli as a post-translational modification. Such removal of the N-terminal methionine yields a polypeptide with the following sequence (SEQ ID NO:20).


Purification



[0099] The purification of the SEQ ID NO:5 or SEQ ID NO:20 polypeptide from E. coli resulted in approximately 5 mg of protein per liter of induced culture. The purification regime outlined in Table 6 took approximately three days to complete. The SEQ ID NO:5/SEQ ID NO:20 polypeptide is overproduced approximately 27-fold in E. coli. Although in the course of the purification trial, the SEQ ID NO: 5/ SEQ ID NO:20 polypeptide was visualized solely by SDS PAGE analysis, it was also useful to define a unit of activity. This calculation helps to assess the SEQ ID NO:5/ SEQ ID NO:20 polypeptide yield and helps quantify activity.

[0100] The purification scheme is aided by the fact that the SEQ ID NO:5/ SEQ ID NO:20 protein is isolated from bacteria as a maltose binding protein (MBP) fusion. Since MBP has a high solubility and affinity for amylose, it is straightforward to express and purify the protein. Preparation of the crude bacterial extract is efficiently achieved by chemical lysis of the bacteria followed by clearing the lysate via centrifugation. The fusion protein was therefore purified to homogeneity in a single step (Figure 3, lane 1). Treatment of this complex with the protease Factor Xa, resulted in full cleavage of the fusion product in approximately 12 hours. There was no detectable proteolysis of the SEQ ID NO:5/ SEQ ID NO:20 protein. The final chromatographic step using MonoQ ion exchange efficiently separated MBP, the SEQ ID NO:5 (SEQ ID NO:20) polypeptide, and Factor Xa. Figure 3 (lane 2) shows the final preparation of homogeneous SEQ ID NO:5/ SEQ ID NO:20 polypeptide after elution from the MonoQ column.
Table 6: Purification of SEQ ID NO:5/ SEQ ID NO:20 polypeptide
Starting material was 5g of E. coli, post induction.
FractionStepConcentration (mg/mL)Total Protein (mg)Specific Act.a (units/mg)Purification (n- fold)
I. Crude extract 30 125 327 1
II. Amylose resin 1.0 25.0 4123 13
III. Factor Xa cleavage 1.1 25.0 8322 25
IV. Mono Q 10 5.2 8747 27
aA unit of the SEQ ID NO:5 polypeptide is defined as the concentration of protein (in µg/mL) that is required to inhibit MMP-9 by 50% in the standard assay.

Antibody Production



[0101] Polyclonal Antisera was prepared against the SEQ ID NO:5 polypeptide in rabbits. A pool of purified antibodies was obtained that readily detects purified SEQ ID NO:5 polypeptide in ELISA reactions (Figure 4). These antibodies can be used to detect and to track the SEQ ID NO:5 polypeptide when it is introduced into chronic wound environments. The antibody pool routinely detected the SEQ ID NO:5 polypeptide using dilutions of approximately 1:5,000.

Matrix Metalloproteinase Inhibition



[0102] The SEQ ID NO:5 polypeptide effectively inhibited the hydrolysis of fluorescinated collagen by MMP-9. When the protein was added to an ongoing enzymatic reaction (Figure 5), 98% of collagen hydrolysis ceased within a 45 second lag period. Titrating MMP-9 with increasing amounts of the SEQ ID NO:5 polypeptide (Figure 6) resulted in loss of MMP-9 hydrolytic activity in a concentration dependent manner. These data indicated that the inhibition reaction is stoichiometric, an observation that was further confirmed in later experiments (see below).

[0103] Using kinetic data shown in Figure 6, was possible to obtain inhibitory constants (Ki) for a host of MMP enzymes. The instantaneous velocities from the fluorescence vs. time plots were used to construct linear Dixon plots, from which it was possible to solve for Ki directly. This analysis assumes that the SEQ ID NO:5 polypeptide functions through a competitive inhibitor mechanism.

[0104] Figure 7 illustrates the SEQ ID NO:5 polypeptide Ki values for five MMP enzymes. All the enzymes were effectively inhibited in the nanomolar range. Surprisingly, the SEQ ID NO:5 polypeptide had a lower Ki value for MMP-1 than it did for MMP-9 (12 vs. 16 nM). However, the low Ki values obtained for all the matrix metalloproteinases tested indicated that the SEQ ID NO:5 polypeptide is capable of preventing the enzymatic activity of all of the major MMP forms that are found in chronic wounds.

Inhibitor Stability



[0105] Figure 8 provides a structural model of the polypeptide backbone of the SEQ ID NO:5 polypeptide and of selected amino acid side chains. This Figure illustrates two important features of the SEQ ID NO:5 polypeptide. The first is the position of the three disulfide bonds that contribute to the stability of the molecule (see below). The second is the position of the single tryptophan molecule that is utilized as the basis for all the intrinsic fluorescence experiments.

[0106] The SEQ ID NO:5 polypeptide unfolds in a highly cooperative manner. Equilibrium unfolding monitored by intrinsic tryptophan fluorescence provided an overall 60 percent decrease in emission fluorescence intensity and a 10 nm shift in the emission peak maximum to longer wavelengths (data not shown).

[0107] Fluorescence intensity emission spectra were converted into the fraction of unfolded protein as described in Example 1. Figure 9 shows that the midpoint in the unfolding curve for native SEQ ID NO:5 polypeptide occurred at a concentration of 4.95 M urea. The unfolding transition began at 4.4 M urea and was complete at a denaturant concentration of 5.4 M urea. The existence of a single peak in the first derivative plot of this data (not shown) supported the hypothesis that the protein denatures as a highly cooperative two state process.

[0108] Conversion of the unfolding curve into a free energy versus the concentration of urea plot (see Example 1) and extrapolation via a linear regression to the free energy in the absence of urea indicated that the polypeptide inhibitor has a native free energy of 7.4 kcal mol-1. When the polypeptide inhibitor was reduced with dithiothreitol prior to the denaturation experiments, there was a significant loss of stability. The unfolding transition then began at 2.4 M urea and was completed at a denaturant concentration of 4.4 M urea, with a transition midpoint of 2.75 M urea. The unfolding process was still a highly cooperative, two-state process.

[0109] The reduced SEQ ID NO:5 polypeptide has a native free energy of 4.3 kcal mol-1. Therefore the three disulfide bonds in the SEQ ID NO:5 polypeptide protein contribute approximately 3.1 kcal mol-1 of stabilization energy.

[0110] The SEQ ID NO:5 protein was long-lived in human serum. Incubation of the SEQ ID NO:5 polypeptide in human serum was performed to simulate exposure of the polypeptide to the types of fluids present in a chronic wound. Incubating the SEQ ID NO:5 polypeptide with human plasma at room temperature over the course of 36 hours resulted in only a 9 percent loss of SEQ ID NO:5 polypeptide (Figure 10). If the SEQ ID NO:5 polypeptide is pre-bound to a stoichiometric amount of MMP-9, then only 4 percent of the material was lost over the course of the same 36 hours. A control reaction, where the SEQ ID NO:5 polypeptide was incubated in PBS, resulted in 100 percent of the material remaining after 36 hours of incubation. The stability of the SEQ ID NO:5 polypeptide was further indicated by the chemical denaturation studies. However, the serum stability indicated that the SEQ ID NO:5 polypeptide may be insensitive to protease degradation. Stability and protease resistance is important in a chronic wound environment.

[0111] The thermal unfolding transition of the SEQ ID NO:5 polypeptide was monitored by intrinsic tryptophan fluorescence. The thermal transition curve is presented in Figure 11. The intrinsic tryptophan fluorescence of the SEQ ID NO:5 polypeptide showed little variation between 25 and 60 °C, consistent with a thermostable native conformation at temperatures below the thermal transition point. At temperatures beyond 60 °C, the SEQ ID NO:5 polypeptide unfolded in a highly cooperative manner. The thermally induced structural transitions were fully reversible at the heating/cooling rates performed in this study (data not shown). The melting behavior of the SEQ ID NO:5 polypeptide was an enthalpic process rather than as an entropic process. The thermodynamic stability parameters are presented in Table 7.
Table 7: Thermodynamic Stability Parameters
ParameterValue
Chemical:  
ΔGnat (kcal mol-1) 7.42
ΔGred (kcal mol-1) 4.32
ΔΔG (kcal mol-1) 3.10
mnat (cal mol-1M-1) 3084
mred (cal mol-1M-1) 3112
urea1/2nat (M) 4.95
urea1/2red (M) 2.75
   
Thermal: 
Tm(C°) 71.5
ΔHm (kcal mol-1) 100
ΔSm (cal mol-1K-1) 250
ΔG71.50C(kcal mol-1) 1.32
ΔG30oC (kcal mol-1) 6.71
Tmax (°C) 37.8
ΔCp (kcal mol-1K-1) 2.84


[0112] The stability of the SEQ ID NO:5 polypeptide as a function of temperature was determined using the Gibbs-Helmholtz function (eq 7), and is presented as ΔG versus temperature in Figure 12. The ΔG values determined at lower temperatures by chemical denaturation in the presence of urea are included for comparison. These lower temperature chemical denaturation studies were also assayed by intrinsic tryptophan fluorescence (see Figure 9). Stability differences persisted over the entire temperature range measured in this study.

[0113] A van't Hoff plot, which illustrates the equilibria constants (KD) determined by intrinsic tryptophan fluorescence, is provided in Figure 13. The calculated temperature of maximum stability of 37.8 °C was ideal for a polypeptide that is to be introduced into wounds or in other physiological environments.

Protein-Protein Interactions



[0114] The chromatographic behavior of the SEQ ID NO:5 polypeptide on the BioSelect 125 gel exclusion column was consistent with the expected monomeric protein. Results of an analytical gel filtration experiment are shown in Figure 14. In this experiment, the SEQ ID NO:5 protein eluted from the column slightly later than a myoglobin standard (12 kDa) The elution profile was consistent with the SEQ ID NO:5 polypeptide being a monomeric protein with a molecular weight of approximately 11.8 kDa.

[0115] The calculated Stokes radius was 22 A. This value is in good agreement with the dimensions of the atomic model. The elution profile suggested that the protein is primarily symmetric in nature because the frictional coefficient was 1.2. However, a frictional coefficient of 1.2 does indicate that the SEQ ID NO:5 polypeptide has a slightly oblate spheroid character, which may indicate that the loop region plays a part in determining the hydrodynamic properties of the protein.

[0116] Complex formation between the SEQ ID NO:5 polypeptide and MMP-9 was determined in three separate experiments.

[0117] In the first experiment, the atomic coordinate files for both molecules were used as input into the program FTDOCK (Gabb et al., 1977). The program calculated molecular surfaces for both molecules, then it held one molecule fixed while it performed a rigid body rotation of the second molecule about the first. For each orientation a fit score is calculated that takes both geometric and electrostatic considerations into account. Finally a series of best orientation structures was provided for inspection. The most probable molecular association between these two molecules is shown in Figure 15. Note that the SEQ ID NO:5 polypeptide makes a significant contact with the matrix metalloproteinase along the planer proposed binding region. Moreover, the flexible loop region of the SEQ ID NO:5 polypeptide also has specific contacts with the matrix metalloproteinase. The structure determined for the complex buried approximately 300 Å2 of the SEQ ID NO:5 polypeptide surface area.

[0118] In the second experiment, the molecular association between the SEQ ID NO:5 polypeptide and matrix metalloproteinase-9 (MMP-9) was measured directly using the technique of surface plasmon resonance. For this experiment, MMP-9 was coupled to the surface of a carboxymethylated dextran sensor chip. A solution of SEQ ID NO:5 polypeptide in PBS was permitted to flow freely over the MMP-9-bound surface. Figure 16 shows the binding isotherm for this interaction. The curve could be fit to an association-disassociation model where the forward (ka) and the reverse (kd) rate constants were fit simultaneously. Such a fit resulted in a ka value of 2 x 105 M-1s-1, and a kd value of 1.3 x 10-3 s-1. This resulted in an equilibrium affinity constant (KA) of 1.5 x 108 M-1.

[0119] In the third experiment, analytical gel filtration was utilized to visualize pre-formed SEQ ID NO:5 polypeptide-MMP-9 complexes. Stoichiometric amounts of both proteins (1 mM) were mixed together and were allowed to incubate at room temperature. After 30 minutes, the entire reaction was injected onto a BioSelect 125 gel filtration column. The mixture eluted from this column as a single molecular weight species of 80 kDa apparent molecular weight (Figure 17). These data indicate that the SEQ ID NO:5 polypeptide binds to MMP-9 in a 1:1 stoichiometry. As a control experiment, the two proteins were mixed together and immediately injected onto the column in order to show the individual protein elution positions. As can be seen in Figure 17, there is a detectable amount of complex formed under these conditions.

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Saarialho-Kere, U.K. (1998). Patterns of matrix metalloproteinase and TIMP expression in chronic ulcers. Arch. Dermatol. Res. 290 (suppl), 47-54.

Sambrook, J., Fritch, EF., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

Sanger, F., Nicklen, S., and Coulson, A.R. (1977). DNA sequencing with chain terminating inhibitors. Proc. Nat. Acad. Sci. U.S.A. 74, 5643-5647.

Sayle, R.A. and Milner-White, E.J. (1995). RasMol: Biomolecular graphics for all. Trends in Biochemical Sciences 20, 374-376.

Segel, IH. (1993) Enzyme Kinetics: Behavior and analysis of rapid equilibrium and steady-state enzyme systems. Wiley Classics Library, John Wiley and Sons, Inc. New York.

Siegel, LM., and Monty, KJ. (1966). Determination of molecular weights and frictional ratios of proteins in impure systems by the use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases. Biochim. Biophys. Acta 112, 346-362.

Su, J-L., Becherer, D., Edwards, C., Bukhart, W., McMgeehan, G.M., and Champion, B.R. (1995). Monoclonal antibodies against human collagenase and stromelysin. Hybridoma. 14, 383-390.

Taylor, K.B., Windsor, J.L., Caterina, N.C.M., Bodden, M.K., and Engler, J.A. (1996). The mechanism of inhibition of collagenase by TIMP-1. J. Biol. Chem. 271, 23938-23945.

Tuuttila, A., Morgunov, E., Bergmann, U., Lindqvist, Y., Maskos, K., Fernandez-Catalan, C., Bode, W., Tryggvason, K., and Schneider, G. (1998). Three dimensional structure of human tissue inhibitor of metalloproteinases-2 at 2.1 Å resolution. J. Mol. Biol. 284, 1133-1140.

Vaalamo, M., Weckroth, M., Puolakkainen, P., Kere, J., Saarinen, P., Lauharanta, J., and Saarialho-Kere, U.K. (1996). Patterns of matrix metalloproteinase and TIMP-1 expression in chronic and normally healing human cutaneous wounds. Brit. J. Dermatol. 135, 52-59.

Vaalamo, M., Mattila, L., Johansson, N., Kariniemi, A-L., Karjalainen-Lindsberg, M-L., Kahari, V-M., and Saarialho-Kere, U.K. (1997). Distinct populations of stromal cells express collagenase-3 (MMP-13) and collagenase-1 (MMP-1) in chronic ulcers, but not in normally healing wounds. J. Investig. Dermatol. 109, 96-101.

Vallon, R., Muller, R., Moosmayer, D., Gerlach, E., and Angel, P. (1997). The catalytic domain of activated collagenase I (MMP-1) is absolutely required for interaction with its specific inhibitor, tissue inhibitor of metalloproteinase-1 (TIMP-1). Eur. J. Biochem. 244, 81-88.

Weckroth, M., Vaheri, A., Lauharanta, J., Sorsa, T., and Konttinen, Y.T. (1996). Matrix metalloproteinases, gelatinases, and collagenases in chronic leg ulcers. J. Investig. Dermatol. 108, 1119-1124.

Wingfield, P.T., Sax, J.K., Stahl, S.J., Kaufman, J., Palmer, I., Chung, V., Corcoran, M.L., Kleiner, D.E., and Stetler-Stevenson, W.G. (1999). Biophysical and Functional characterization of full-length recombinant human tissue inhibitor of metalloproteinase-2 (TIMP-2) produced in E. coli. J. Biol. Chem. 274, 21362-21368.

Wojtowicz-Praga, S.M., Dickson, R.B., and Hawkins, M.J. (1997). Matrix metalloproteinase inhibitors. Investigational New Drugs. 15, 61-75.


SEQUENCE LISTING



[0121] 

<110> Kimberly-Clark Worldwide, Inc.
Stephen Quirk

<120> Wound Care Compositions

<130> 15,420

<140> US 10/325,446
<141> 2002-12-19

<160> 21

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 207
<212> PRT
<213> Homo sapiens

<400> 1



<210> 2
<211> 220
<212> PRT
<213> Homo sapiens

<400> 2

<210> 3
<211> 211
<212> PRT
<213> Homo sapiens

<400> 3

<210> 4
<211> 224
<212> PRT
<213> Homo sapiens

<400>

<210> 5
<211> 108
<212> PRT
<213> Artificial Sequence

<220>
<223> A synthetic polypeptide inhibitor.

<400> 5



<210> 6
<211> 339
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic nucleic acid sequence of a polypeptide inhibitor.

<400> 6

<210> 7
<211> 108
<212> PRT
<213> Artificial Sequence

<220>
<223> A synthetic polypeptide inhibitor.

<220>
<221> SITE
<222> 7, 12, 16, 18-20, 22, 24-25, 30, 36, 41, 44, 48, 51, 61, 64, 67, 71-72, 75, 77, 79, 87-88, 91, 99, 101, 105
<223> Xaa = any aliphatic amino acid, alanine, valine, isoleucine or leucine

<220>
<221> SITE
<222> 17, 27, 29, 31, 35, 47, 58, 60, 62, 78, 84, 92, 94, 103
<223> Xaa = any acidic amino acid, aspartic acid or glutamic acid

<220>
<221> SITE
<222> 2, 4, 73, 86, 102, 106
<223> Xaa = any cysteine-like amino acid or cysteine

<220>
<221> SITE
<222> 21, 23, 28, 42-43, 49, 52, 55, 59, 82-83, 90, 96
<223> Xaa = any basic amino acid, lysine or arginine

<220>
<221> SITE
<222> 13, 54, 63, 104
<223> Xaa = any aromatic amino acid or phenylalanine

<220>
<221> SITE
<222> (1)...(1)
<223> Xaa = any apolar amino acid, methionine, or no amino acid

<220>
<221> SITE
<222> (53)...(53)
<223> Xaa = any apolar amino acid or methionine

<220>
<221> SITE
<222> (97) . . . (97)
<223> Xaa = any apolar amino acid or methionine

<220>
<221> SITE
<222> (6)... (6)
<223> Xaa = any apolar amino acid or proline

<220>
<221> SITE
<222> (9)...(9)
<223> Xaa = any apolar amino acid or proline

<220>
<221> SITE
<222> (33)...(33)
<223> Xaa = any apolar amino acid or glycine

<220>
<221> SITE
<222> (38) . . . (38)
<223> Xaa = any apolar amino acid or glycine

<220>
<221> SITE
<222> (40)...(40)
<223> Xaa = any apolar amino acid or proline

<220>
<221> SITE
<222> (56) . . . (56)
<223> Xaa = any apolar amino acid or glycine

<220>
<221> SITE
<222> (57)...(57)
<223> Xaa = any apolar amino acid or proline

<220>
<221> SITE
<222> (68)...(68)
<223> Xaa = any apolar amino acid or proline

<220>
<221> SITE
<222> (74)...(74)
<223> Xaa = any apolar amino acid or glycine

<220>
<221> SITE
<222> (80) . . . (80)
<223> Xaa = any apolar amino acid or glycine

<220>
<221> SITE
<222> (81) . . . (81)
<223> Xaa = any apolar amino acid or glycine

<220>
<221> SITE
<222> (89)...(89)
<223> Xaa = any apolar amino acid or glycine

<220>
<221> SITE
<222> (93) . . . (93)
<223> Xaa = any apolar amino acid or glycine

<220>
<221> SITE
<222> (95)...(95)
<223> Xaa = any apolar amino acid or glycine

<220>
<221> SITE
<222> (107)...(107)
<223> Xaa = any apolar amino acid or proline

<220>
<221> SITE
<222> (3) . . . (3)
<223> Xaa = any polar amino acid, serine or threonine

<220>
<221> SITE
<222> (5) . . . (5)
<223> Xaa = any polar amino acid, serine or threonine

<220>
<221> SITE
<222> (10) . . . (10)
<223> Xaa = any polar amino acid, asparagine, or glutamine

<220>
<221> SITE
<222> (11)...(11)
<223> Xaa = any polar amino acid, asparagine, or glutamine

<220>
<221> SITE
<222> (14)...(14)
<223> Xaa = any polar amino acid, serine or threonine

<220>
<221> SITE
<222> (15)...(15)
<223> Xaa = any polar amino acid, asparagine, or glutamine

<220>
<221> SITE
<222> (26) . . . (26)
<223> Xaa = any polar amino acid, serine or threonine

<220>
<221> SITE
<222> (32)...(32)
<223> Xaa = any polar amino acid, serine or threonine

<220>
<221> SITE
<222> (34) . . . (334)
<223> Xaa = any polar amino acid, asparagine, or glutamine

<220>
<221> SITE
<222> (37)...(37)
<223> Xaa = any polar amino acid or tyrosine

<220>
<221> SITE
<222> (39)...(39)
<223> Xaa = any polar amino acid, asparagine, or glutamine

<220>
<221> SITE
<222> (45)...(45)
<223> Xaa = any polar amino acid, asparagine, or glutamine

<220>
<221> SITE
<222> (46) ... (46)
<223> Xaa = any polar amino acid or tyrosine

<220>
<221> SITE
<222> (50) . . . (50)
<223> Xaa = any polar amino acid, asparagine, or glutamine

<220>
<221> SITE
<222> (65)...(65)
<223> Xaa = any polar amino acid or tyrosine

<220>
<221> SITE
<222> (66)...(66)
<223> Xaa = any polar amino acid, serine or threonine

<220>
<221> SITE
<222> (69)...(69)
<223> Xaa = any polar amino acid, serine or threonine

<220>
<221> SITE
<222> (70)...(70)
<223> Xaa = any polar amino acid, serine or threonine

<220>
<221> SITE
<222> (76)...(76)
<223> Xaa = any polar amino acid, serine or threonine

<220>
<221> SITE
<222> (85)...(85)
<223> Xaa = any polar amino acid or tyrosine

<220>
<221> SITE
<222> (100)...(100)
<223> Xaa = any polar amino acid, serine or threonine

<220>
<221> SITE
<222> (8) . . . (8)
<223> Xaa = any basic amino acid or histidine

<220>
<221> SITE
<222> (98)...(98)
<223> Xaa = any basic amino acid or histidine

<220>
<221> SITE
<222> (108)...(108)
<223> Xaa = tryptophan

<400> 7

<210> 8
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 8

<210> 9
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 9

<210> 10
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 10

<210> 11
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 11

<210> 12
<211> 59
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 12
atggcaaaat gcatattacc ctgtgcgatt ttatttgccc gtggtagaag cttatagac   59

<210> 13
<211> 80
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 13

<210> 14
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 14

<210> 15
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 15

<210> 16
<211> 70
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 16

<210> 17
<211> 49
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 17
gtctataagc ttctaccacg ggcaaataaa atcgcacagg gtaatatgc 49

<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 18
atgtgcagct gcagcccggt   20

<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> A synthetic primer.

<400> 19
gtctataagc ttctaccacg   20

<210> 20
<211> 107
<212> PRT
<213> Artificial Sequence

<220>
<223> A synthetic polypeptide inhibitor.

<400> 20

<210> 21
<211> 108
<212> PRT
<213> Artificial Sequence

<220>
<223> A synthetic polypeptide inhibitor.

<220>
<221> SITE
<222> 7, 12, 16, 18-20, 22, 24-25, 30, 36, 41, 44, 48, 51, 61, 64, 67, 71-72, 75, 77, 79, 87-88, 91, 99, 101, 105
<223> Xaa = alanine, valine, isoleucine, or leucine

<220>
<221> SITE
<222> 17, 27, 29, 31, 35, 47, 58, 60, 62, 78, 84, 92, 94, 103
<223> Xaa = aspartic acid or glutamic acid

<220>
<221> SITE
<222> 3, 5, 14, 26, 32, 66, 69, 70, 76, 100
<223> Xaa = serine or threonine

<220>
<221> SITE
<222> 21, 23, 28, 42-43, 49, 52, 55, 59, 82-83, 90, 96
<223> Xaa = lysine or arginine

<220>
<221> SITE
<222> 33, 38, 56, 74, 80, 81, 89, 93, 95
<223> Xaa = glycine

<220>
<221> SITE
<222> (1) . . . (1)
<223> Xaa = methionine

<220>
<221> SITE
<222> (53) . . . (53)
<223> Xaa = methionine

<220>
<221> SITE
<222> (97)...(97)
<223> Xaa = methionine

<220>
<221> SITE
<222> (2)...(2)
<223> Xaa = cysteine

<220>
<221> SITE
<222> (4) . . . (4)
<223> Xaa = cysteine

<220>
<221> SITE
<222> (73) . . . (73)
<223> Xaa = cysteine

<220>
<221> SITE
<222> (86)...(86)
<223> Xaa = cysteine

<220>
<221> SITE
<222> (102)...(102)
<223> Xaa = cysteine

<220>
<221> SITE
<222> (106) . . . (106)
<223> Xaa = cysteine

<220>
<221> SITE
<222> (8) . . . (8)
<223> Xaa = histidine

<220>
221> SITE

<222> (98) . . . (98)
<223> Xaa = histidine

<220>
<221> SITE
<222> (6) . . . (6)
<223> Xaa = proline

<220>
<221> SITE
<222> (9) . . . (9)
<223> Xaa = proline

<220>
<221> SITE
<222> (40)...(40)
<223> Xaa = proline

<220>
<221> SITE
<222> (57)...(57)
<223> Xaa = proline

<220>
<221> SITE
<222> (68)...(68)
<223> Xaa = proline

<220>
<221> SITE
<222> (107)...(107)
<223> Xaa = proline

<220>
<221> SITE
<222> (10)...(10)
<223> Xaa = asparagine or glutamine

<220>
<221> SITE
<222> (11)...(11)
<223> Xaa = asparagine or glutamine

<220>
<221> SITE
<222> (15)...(15)
<223> Xaa = asparagine or glutamine

<220>
<221> SITE
<222> (34)...(34)
<223> Xaa = asparagine or glutamine

<220>
<221> SITE
<222> (39)...(39)
<223> Xaa = asparagine or glutamine

<220>
<221> SITE
<222> (45)...(45)
<223> Xaa = asparagine or glutamine

<220>
<221> SITE
<222> (50)... (50)
<223> Xaa = asparagine or glutamine

<220>
<221> SITE
<222> (13)...(13)
<223> Xaa = phenylalanine

<220>
<221> SITE
<222> (54)...(54)
<223> Xaa = phenylalanine

<220>
<221> SITE
<222> (63)...(63)
<223> Xaa = phenylalanine

<220>
<221> SITE
<222> (104)...(104)
<223> Xaa = phenylalanine

<220>
<221> SITE
<222> (37)...(37)
<223> Xaa = tyrosine

<220>
<221> SITE
<222> (46)...(46)
<223> Xaa = tyrosine

<220>
<221> SITE
<222> (65)...(65)
<223> Xaa = tyrosine

<220>
<221> SITE
<222> (85)...(85)
<223> Xaa = tyrosine

<220>
<221> SITE
<222> (108)...(108)
<223> Xaa = tyrosine

<400> 21




Claims

1. An isolated nucleic acid that encodes a polypeptide shorter than 200 amino acids and comprising SEQ ID NO:5 or SEQ ID NO:20, wherein the polypeptide inhibits matrix metalloproteinase activity.
 
2. The isolated nucleic acid of claim 1, wherein the nucleic acid comprises SEQ ID NO:6.
 
3. An isolated polypeptide shorter than 200 amino acids and comprising SEQ ID NO:5 or SEQ ID NO:20, wherein the polypeptide inhibits matrix metalloproteinase activity.
 
4. The isolated polypeptide of claim 3, wherein the polypeptide is stable in mammalian serum or plasma.
 
5. The isolated polypeptide of claim 3, wherein substantially all of the polypeptide remains folded in a beta barrel conformation while in 4M urea.
 
6. A composition that comprises a therapeutically effective amount of polypeptide inhibitor shorter than 200 amino acids and comprising SEQ ID NO:5 or SEQ ID NO:20 and a pharmaceutically acceptable carrier, wherein the polypeptide inhibits matrix metalloproteinase activity.
 
7. The composition of claim 6 wherein the polypeptide inhibitor can inhibit proteinase activity of any one of matrix metalloproteinase-1, matrix metalloproteinase-2, matrix metalloproteinase-3, matrix metalloproteinase-4, matrix metalloproteinase-5, matrix metalloproteinase-6, matrix metalloproteinase-7, matrix metalloproteinase-8, and matrix metalloproteinase-9, matrix metalloproteinase-10, matrix metalloproteinase-11, matrix metalloproteinase-12, or matrix metalloproteinase-13.
 
8. The composition of claim 6, wherein the polypeptide inhibitor can inhibit more than one of matrix metalloproteinase-1, matrix metalloproteinase-2, matrix metalloproteinase-3, matrix metalloproteinase-4, matrix metalloproteinase-5, matrix metalloproteinase-6, matrix metalloproteinase-7, matrix metalloproteinase-8, and matrix metalloproteinase-9, matrix metalloproteinase-10, matrix metalloproteinase-11, matrix metalloproteinase-12, or matrix metalloproteinase-13.
 
9. The composition of claim 6, wherein the polypeptide inhibitor has a beta barrel conformation.
 
10. The composition of claim 6, wherein the polypeptide is stable in mammalian serum.
 
11. The composition of claim 6, wherein substantially all of the polypeptide remains folded in a beta barrel conformation while in 4M urea.
 
12. The composition of claim 6, wherein the composition comprises a lotion, gel or cream.
 
13. A wound dressing that comprises a polypeptide shorter than 200 amino acids and comprising SEQ ID NO:5 or SEQ ID NO:20 and a pharmaceutically acceptable carrier, wherein the polypeptide inhibits matrix metalloproteinase activity.
 
14. The wound dressing of claim 13, wherein the polypeptide can inhibit proteinase activity of any one of matrix metalloproteinase-1, matrix metalloproteinase-2, matrix metalloproteinase-3, matrix metalloproteinase-4, matrix metalloproteinase-5, matrix metalloproteinase-6, matrix metalloproteinase-7, matrix metalloproteinase-8, and matrix metalloproteinase-9, matrix metalloproteinase-10, matrix metalloproteinase-11, matrix metalloproteinase-12, or matrix metalloproteinase.
 
15. The wound dressing of claim 13, wherein the polypeptide can inhibit more than one of matrix metalloproteinase-1, matrix metalloproteinase-2, matrix metalloproteinase-3, matrix metalloproteinase-4, matrix metalloproteinase-5, matrix metalloproteinase-6, matrix metalloproteinase-7, matrix metalloproteinase-8, and matrix metalloproteinase-9, matrix metalloproteinase-10, matrix metalloproteinase-11, matrix metalloproteinase-12, or matrix metalloproteinase-13.
 
16. The wound dressing of claim 13, wherein the polypeptide has a beta barrel conformation.
 
17. The wound dressing of claim 13, wherein the polypeptide is stable in mammalian serum.
 
18. The wound dressing of claim 13, wherein substantially all of the polypeptide remains folded in a beta barrel conformation while in 4M urea.
 
19. The wound dressing of claim 13, wherein the pharmaceutically acceptable carrier is a bandage.
 
20. A polypeptide shorter than 200 amino acids and comprising SEQ ID NO:5 or SEQ ID NO:20, for use in treating a wound, wherein the polypeptide inhibits matrix metalloproteinase activity.
 
21. The polypeptide of claim 20, wherein the polypeptide can inhibit proteinase activity of any one of matrix metalloproteinase-1, matrix metalloproteinase-2, matrix metalloproteinase-3, matrix metalloproteinase-4, matrix metalloproteinase-5, matrix metalloproteinase-6, matrix metalloproteinase-7, matrix metalloproteinase-8, and matrix metalloproteinase-9, matrix metalloproteinase-10, matrix metalloproteinase-11, matrix metalloproteinase-12, or matrix metalloproteinase.
 
22. The polypeptide of claim 20, wherein the polypeptide can inhibit more than one of matrix metalloproteinase-1, matrix metalloproteinase-2, matrix metalloproteinase-3, matrix metalloproteinase-4, matrix metalloproteinase-5, matrix metalloproteinase-6, matrix metalloproteinase-7, matrix metalloproteinase-8, and matrix metalloproteinase-9, matrix metalloproteinase-10, matrix metalloproteinase-11, matrix metalloproteinase-12, or matrix metalloproteinase-13.
 
23. The polypeptide of claim 20, wherein the polypeptide has a beta barrel conformation.
 
24. The polypeptide of claim 20, wherein the polypeptide is stable in mammalian serum.
 
25. The polypeptide of claim 20, wherein substantially all of the polypeptide remains folded in a beta barrel conformation while in 4M urea.
 


Ansprüche

1. Isolierte Nucleinsäure, die für ein Polypeptid codiert, das kürzer als 200 Aminosäuren ist und SEQ ID NO: 5 oder SEQ ID NO:20 beinhaltet, wobei das Polypeptid die Matrixmetalloproteinaseaktivität hemmt.
 
2. Isolierte Nucleinsäure gemäß Anspruch 1, wobei die Nucleinsäure SEQ ID NO:6 beinhaltet.
 
3. Isoliertes Polypeptid, das kürzer als 200 Aminosäuren ist und SEQ ID NO: 5 oder SEQ ID NO:20 beinhaltet, wobei das Polypeptid die Matrixmetalloproteinaseaktivität hemmt.
 
4. Isoliertes Polypeptid gemäß Anspruch 3, wobei das Polypeptid in Säugerserum oder -plasma stabil ist.
 
5. Isoliertes Polypeptid gemäß Anspruch 3, wobei im Wesentlichen das gesamte Polypeptid in einer β-Tonnen-Konformation gefaltet bleibt, während es sich in 4 M Harnstoff befindet.
 
6. Zusammensetzung, die eine therapeutisch wirksame Menge an Polypeptidhemmer, der kürzer als 200 Aminosäuren ist und SEQ ID NO:5 oder SEQ ID NO:20 beinhaltet, und einen pharmazeutisch akzeptablen Träger beinhaltet, wobei das Polypeptid die Matrixmetalloproteinaseaktivität hemmt.
 
7. Zusammensetzung gemäß Anspruch 6, wobei der Polypeptidhemmer die Proteinaseaktivität einer von Matrixmetalloproteinase-1, Matrixmetalloproteinase-2, Matrixmetalloproteinase-3, Matrixmetalloproteinase-4, Matrixmetalloproteinase-5, Matrixmetalloproteinase-6, Matrixmetalloproteinase-7, Matrixmetalloproteinase-8 und Matrixmetalloproteinase-9, Matrixmetalloproteinase-10, Matrixmetalloproteinase-11, Matrixmetalloproteinase-12 oder Matrixmetalloproteinase-13 hemmen kann.
 
8. Zusammensetzung gemäß Anspruch 6, wobei der Polypeptidhemmer mehr als eine von Matrixmetalloproteinase-1, Matrixmetalloproteinase-2, Matrixmetalloproteinase-3, Matrixmetalloproteinase-4, Matrixmetalloproteinase-5, Matrixmetalloproteinase-6, Matrixmetalloproteinase-7, Matrixmetalloproteinase-8 und Matrixmetalloproteinase-9, Matrixmetalloproteinase-10, Matrixmetalloproteinase-11, Matrixmetalloproteinase-12 oder Matrixmetalloproteinase-13 hemmen kann.
 
9. Zusammensetzung gemäß Anspruch 6, wobei der Polypeptidhemmer eine β-Tonnen-Konformation aufweist.
 
10. Zusammensetzung gemäß Anspruch 6, wobei das Polypeptid in Säugerserum stabil ist.
 
11. Zusammensetzung gemäß Anspruch 6, wobei im Wesentlichen das gesamte Polypeptid in einer β-Tonnen-Konformation gefaltet bleibt, während es sich in 4 M Harnstoff befindet.
 
12. Zusammensetzung gemäß Anspruch 6, wobei die Zusammensetzung eine Lotion, ein Gel oder eine Creme beinhaltet.
 
13. Wundauflage, die ein Polypeptid, das kürzer als 200 Aminosäuren ist und SEQ ID NO: 5 oder SEQ ID NO:20 beinhaltet, und einen pharmazeutisch akzeptablen Träger beinhaltet, wobei das Polypeptid die Matrixmetalloproteinaseaktivität hemmt.
 
14. Wundauflage gemäß Anspruch 13, wobei das Polypeptid die Proteinaseaktivität einer von Matrixmetalloproteinase-1, Matrixmetalloproteinase-2, Matrixmetalloproteinase-3, Matrixmetalloproteinase-4, Matrixmetalloproteinase-5, Matrixmetalloproteinase-6, Matrixmetalloproteinase-7, Matrixmetalloproteinase-8 und Matrixmetalloproteinase-9, Matrixmetalloproteinase-10, Matrixmetalloproteinase-11, Matrixmetalloproteinase-12 oder Matrixmetalloproteinase-13 hemmen kann.
 
15. Wundauflage gemäß Anspruch 13, wobei das Polypeptid mehr als eine von Matrixmetalloproteinase-1, Matrixmetalloproteinase-2, Matrixmetalloproteinase-3, Matrixmetalloproteinase-4, Matrixmetalloproteinase-5, Matrixmetalloproteinase-6, Matrixmetalloproteinase-7, Matrixmetalloproteinase-8 und Matrixmetalloproteinase-9, Matrixmetalloproteinase-10, Matrixmetalloproteinase-11, Matrixmetalloproteinase-12 oder Matrixmetalloproteinase-13 hemmen kann.
 
16. Wundauflage gemäß Anspruch 13, wobei das Polypeptid eine β-Tonnen-Konformation aufweist.
 
17. Wundauflage gemäß Anspruch 13, wobei das Polypeptid in Säugerserum stabil ist.
 
18. Wundauflage gemäß Anspruch 13, wobei im Wesentlichen das gesamte Polypeptid in einer β-Tonnen-Konformation gefaltet bleibt, während es sich in 4 M Harnstoff befindet.
 
19. Wundauflage gemäß Anspruch 13, wobei der pharmazeutisch akzeptable Träger ein Verband ist.
 
20. Polypeptid, das kürzer als 200 Aminosäuren ist und SEQ ID NO:5 oder SEQ ID NO:20 beinhaltet, zur Verwendung bei der Behandlung einer Wunde, wobei das Polypeptid die Matrixmetalloproteinaseaktivität hemmt.
 
21. Polypeptid gemäß Anspruch 20, wobei das Polypeptid die Proteinaseaktivität einer von Matrixmetalloproteinase-1, Matrixmetalloproteinase-2, Matrixmetalloproteinase-3, Matrixmetalloproteinase-4, Matrixmetalloproteinase-5, Matrixmetalloproteinase-6, Matrixmetalloproteinase-7, Matrixmetalloproteinase-8 und Matrixmetalloproteinase-9, Matrixmetalloproteinase-10, Matrixmetalloproteinase-11, Matrixmetalloproteinase-12 oder Matrixmetalloproteinase-13 hemmen kann.
 
22. Polypeptid gemäß Anspruch 20, wobei das Polypeptid mehr als eine von Matrixmetalloproteinase-1, Matrixmetalloproteinase-2, Matrixmetalloproteinase-3, Matrixmetalloproteinase-4, Matrixmetalloproteinase-5, Matrixmetalloproteinase-6, Matrixmetalloproteinase-7, Matrixmetalloproteinase-8 und Matrixmetalloproteinase-9, Matrixmetalloproteinase-10, Matrixmetalloproteinase-11, Matrixmetalloproteinase-12 oder Matrixmetalloproteinase-13 hemmen kann.
 
23. Polypeptid gemäß Anspruch 20, wobei das Polypeptid eine β-Tonnen-Konformation aufweist.
 
24. Polypeptid gemäß Anspruch 20, wobei das Polypeptid in Säugerserum stabil ist.
 
25. Polypeptid gemäß Anspruch 20, wobei im Wesentlichen das gesamte Polypeptid in einer β-Tonnen-Konformation gefaltet bleibt, während es sich in 4 M Harnstoff befindet.
 


Revendications

1. Acide nucléique isolé qui code pour un polypeptide plus court que 200 acides aminés et comprenant la SEQ ID n° 5 ou la SEQ ID n° 20, dans lequel le polypeptide inhibe l'activité des matrices métalloprotéinases.
 
2. Acide nucléique isolé selon la revendication 1, dans lequel l'acide nucléique comprend la SEQ ID n° 6.
 
3. Polypeptide isolé plus court que 200 acides aminés et comprenant la SEQ ID n° 5 ou la SEQ ID n° 20, dans lequel le polypeptide inhibe l'activité des matrices métalloprotéinases.
 
4. Polypeptide isolé selon la revendication 3, dans lequel le polypeptide est stable dans le sérum ou le plasma de mammifère.
 
5. Polypeptide isolé selon la revendication 3, dans lequel sensiblement la totalité du polypeptide reste plié en conformation de cylindre bêta tandis qu'il se trouve dans de l'urée 4M.
 
6. Composition qui comprend une quantité thérapeutiquement efficace d'inhibiteur de polypeptide plus court que 200 acides aminés et comprenant la SEQ ID n° 5 ou la SEQ ID n° 20 et un véhicule pharmaceutiquement acceptable, dans lequel le polypeptide empêche l'activité des matrices métalloprotéinases.
 
7. Composition selon la revendication 6, dans laquelle l'inhibiteur de polypeptide peut empêcher l'activité de protéinase de l'une quelconque des matrices suivantes : matrice métalloprotéinase-1, matrice métalloprotéinase-2, matrice métallo-protéinase-3, matrice métalloprotéinase-4, matrice métalloprotéinase-5, matrice métalloprotéinase-6, matrice métalloprotéinase-7, matrice métalloprotéinase-8 et matrice métalloprotéinase-9, matrice métalloprotéinase-10, matrice métalloprotéinase-11, matrice métalloprotéinase-12 ou matrice métalloprotéinase-13.
 
8. Composition selon la revendication 6, dans laquelle l'inhibiteur de polypeptide peut inhiber plus d'une de la matrice métalloprotéinase-1, de la matrice métalloprotéinase-2, de la matrice métalloprotéinase-3, de la matrice métalloprotéinase-4, de la matrice métalloprotéinase-5, de la matrice métalloprotéinase-6, de la matrice métalloprotéinase-7, de la matrice métalloprotéinase-8 et de la matrice métalloprotéinase-9, de la matrice métalloprotéinase-10, de la matrice métalloprotéinase-11, de la matrice métalloprotéinase-11, de la matrice métalloprotéinase-12 ou de la matrice métalloprotéinase-13.
 
9. Composition selon la revendication 6, dans laquelle l'inhibiteur de polypeptide a une conformation de cylindre bêta.
 
10. Composition selon la revendication 6, dans laquelle le polypeptide est stable dans le sérum de mammifère.
 
11. Composition selon la revendication 6, dans laquelle sensiblement la totalité du polypeptide reste plié en conformation de cylindre bêta tandis qu'il se trouve dans de l'urée 4M.
 
12. Composition selon la revendication 6, dans laquelle la composition comprend une lotion, un gel ou une crème.
 
13. Pansement pour plaie, qui comprend un polypeptide plus court que 200 acides aminés et comprenant la SEQ ID n° 5 ou la SEQ ID n° 20 et un véhicule pharmaceutiquement acceptable, dans lequel le polypeptide inhibe l'activité des matrices métalloprotéinases.
 
14. Pansement pour plaie selon la revendication 13, dans lequel le polypeptide peut inhiber l'activité de protéinase de l'une quelconque des matrices suivantes : matrice métalloprotéinase-1, matrice métalloprotéinase-2, matrice métalloprotéinase-3, matrice métalloprotéinase-4, matrice métalloprotéinase-5, matrice métalloprotéinase-6, matrice métalloprotéinase-7, matrice métalloprotéinase-8 et matrice métalloprotéinase-9, matrice métalloprotéinase-10, matrice métalloprotéinase-11, matrice métalloprotéinase-12 ou matrice métalloprotéinase.
 
15. Pansement pour plaie selon la revendication 13, dans lequel le polypeptide peut inhiber plus d'une de la matrice métalloprotéinase-1, de la matrice métalloprotéinase-2, de la matrice métalloprotéinase-3, de la matrice métalloprotéinase-4, de la matrice métalloprotéinase-5, de la matrice métalloprotéinase-6, de la matrice métalloprotéinase-7, de la matrice métalloprotéinase-8 et de la matrice métalloprotéinase-9, de la matrice métalloprotéinase-10, de la matrice métalloprotéinase-11, de la matrice métalloprotéinase-12 ou de la matrice métalloprotéinase-13.
 
16. Pansement pour plaie selon la revendication 13, dans lequel le polypeptide a une conformation de cylindre bêta.
 
17. Pansement pour plaie selon la revendication 13, dans lequel le polypeptide est stable dans le sérum de mammifère.
 
18. Pansement pour plaie selon la revendication 13, dans lequel sensiblement la totalité du polypeptide reste plié en conformation de cylindre bêta tandis qu'il se trouve dans de l'urée 4M.
 
19. Pansement pour plaie selon la revendication 13, dans lequel le véhicule pharmaceutiquement acceptable est un bandage.
 
20. Polypeptide plus court que 200 acides aminés et comprenant la SEQ ID n° 5 et la SEQ ID n° 20 pour un usage dans le traitement d'une plaie, dans lequel le polypeptide inhibe l'activité des matrices métalloprotéinases.
 
21. Polypeptide selon la revendication 20, dans lequel le polypeptide peut inhiber l'activité de protéinase de l'une quelconque des matrices suivantes : matrice métalloprotéinase-1, matrice métalloprotéinase-2, matrice métalloprotéinase-3, matrice métalloprotéinase-4, matrice métalloprotéinase-5, matrice métalloprotéinase-6, matrice métalloprotéinase-7, matrice métalloprotéinase-8 et matrice métalloprotéinase-9, matrice métalloprotéinase-10, matrice métalloprotéinase-11, matrice métalloprotéinase-12 ou matrice métalloprotéinase-13.
 
22. Polypeptide selon la revendication 20, dans lequel le polypeptide peut inhiber plus d'un de la matrice métalloprotéinase-1, de la matrice métalloprotéinase-2, de la matrice métalloprotéinase-3, de la matrice métalloprotéinase-4, de la matrice métalloprotéinase-5, de la matrice métalloprotéinase-6, de la matrice métalloprotéinase-7, de la matrice métalloprotéinase-8 et de la matrice métalloprotéinase-9, de la matrice métalloprotéinase-10, de la matrice métalloprotéinase-11, de la matrice métalloprotéinase-12 ou de la matrice métalloprotéinase-13.
 
23. Polypeptide selon la revendication 20, dans lequel le polypeptide a une conformation de cylindre bêta.
 
24. Polypeptide selon la revendication 20, dans lequel le polypeptide est stable dans le sérum de mammifère.
 
25. Polypeptide selon la revendication 20, dans lequel sensiblement la totalité du polypeptide reste plié en conformation de cylindre bêta tandis qu'il se trouve dans de l'urée 4M.
 




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