(19)
(11)EP 1 564 303 A3

(12)EUROPEAN PATENT APPLICATION

(88)Date of publication A3:
07.06.2006 Bulletin 2006/23

(43)Date of publication A2:
17.08.2005 Bulletin 2005/33

(21)Application number: 04023644.0

(22)Date of filing:  05.05.1998
(51)Int. Cl.: 
C12Q 1/68  (2006.01)
(84)Designated Contracting States:
DE FR GB IT

(30)Priority: 08.05.1997 US 854041

(62)Application number of the earlier application in accordance with Art. 76 EPC:
98108154.0 / 0878553

(71)Applicant: Becton Dickinson and Company
Franklin Lakes, New Jersey 07417 (US)

(72)Inventors:
  • Pearson, Robert E.
    Durham, North Carolina 27713 (US)
  • Dickson, Julie A.
    Raleigh, North Carolina 27612 (US)
  • Mehrpouyan, Majid
    San José, California 95132 (US)

(74)Representative: Comoglio, Elena et al
Jacobacci & Partners S.p.A Corso Emilia, 8
10152 Torino
10152 Torino (IT)

  


(54)Strand displacement amplification of RNA targets


(57) Strand Displacement Amplification has been adapted to reverse transcription amplification of RNA targets. The method of the invention is referred to as reverse transcription SDA (rtSDA) and may be performed as a two-step process or as a one-step process in which cDNA copies of an RNA target sequence are generated and amplified concurrently. In the one-step process, rtSDA reaction conditions are such that a conventional reverse transcriptase and a DNA-dependent polymerase function together in a single reaction to produce cDNA and amplify it.