(19)
(11)EP 1 581 532 B1

(12)EUROPEAN PATENT SPECIFICATION

(45)Mention of the grant of the patent:
28.04.2010 Bulletin 2010/17

(21)Application number: 03789413.6

(22)Date of filing:  19.12.2003
(51)Int. Cl.: 
C07D 471/04  (2006.01)
A61P 29/00  (2006.01)
A61K 31/437  (2006.01)
(86)International application number:
PCT/EP2003/014867
(87)International publication number:
WO 2004/056823 (08.07.2004 Gazette  2004/28)

(54)

PYRAZOLO¬3,4-B PYRIDINE COMPOUNDS, AND THEIR USE AS PHOSPHODIESTERASE INHIBITORS

PYRAZOLO¬3,4-B PYRIDINVERBINDUNGEN UND IHRE VERWENDUNG ALS PHOSPHODIESTERASE INHIBITOREN

COMPOSES DE PYRAZOLO¬3,4-B PYRIDINE ET LEUR UTILISATION EN TANT QU'INHIBITEURS DE PHOSPHODIESTERASE


(84)Designated Contracting States:
AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IT LI LU MC NL PT RO SE SI SK TR
Designated Extension States:
LT LV

(30)Priority: 23.12.2002 GB 0230045
24.12.2002 GB 0230165
07.04.2003 GB 0307998

(43)Date of publication of application:
05.10.2005 Bulletin 2005/40

(73)Proprietor: GLAXO GROUP LIMITED
Greenford, Middlesex UB6 0NN (GB)

(72)Inventors:
  • ALLEN, David George GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • COE, Diane Mary GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • COOK, Caroline Mary GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • COOPER, Anthony William James GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • DOWLE, Michael Dennis GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • EDLIN, Christopher, David GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • HAMBLIN, Julie Nicole GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • JOHNSON ,Martin, Redpath GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • JONES, Paul Spencer GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • LINDVALL, Mika, Kristian GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • MITCHELL, Charlotte, Jane GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)
  • REDGRAVE, Alison, Judith GlaxoSmithKline
    Stevenage, Hertfordshire SG1 2NY (GB)

(74)Representative: Breen, Anthony Paul et al
GlaxoSmithKline Corporate Intellectual Property (CN9.25.1) 980 Great West Road
Brentford, Middlesex TW8 9GS
Brentford, Middlesex TW8 9GS (GB)


(56)References cited: : 
WO-A-00/15222
  
      
    Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).


    Description


    [0001] The present invention relates to pyrazolopyridine compounds, processes for their preparation, and pharmaceutical compositions containing the compounds. The invention also relates to the pyrazolopyridine compounds for use in therapy, for example as inhibitors of phosphodiesterases (PDE) and/or for the treatment and/or prophylaxis of inflammatory and/or allergic diseases such as chronic obstructive pulmonary disease (COPD), asthma, rheumatoid arthritis or allergic rhinitis.

    [0002] US 3,979,399, US 3,840,546, and US 3,966,746 (E.R.Squibb & Sons) disclose 4-amino derivatives of pyrazolo[3,4-b]pyridine-5-carboxamides wherein the 4-amino group NR3R4 can be an acyclic amino group wherein R3 and R4 may each be hydrogen, lower alkyl (e.g. butyl), phenyl, etc.; NR3R4 can alternatively be a 3-6-membered heterocyclic group such as pyrrolidino, piperidino and piperazino. The compounds are disclosed as central nervous system depressants useful as ataractic, analgesic and hypotensive agents.

    [0003] US 3,925,388, US 3,856,799, US 3,833,594 and US 3,755,340 (E.R.Squibb & Sons) disclose 4-amino derivatives of pyrazolo[3,4-b]pyridine-5-carboxylic acids and esters. The 4-amino group NR3R4 can be an acyclic amino group wherein R3 and R4 may each be hydrogen, lower alkyl (e.g. butyl), phenyl, etc.; NR3R4 can alternatively be a 5-6-membered heterocyclic group in which an additional nitrogen is present such as pyrrolidino, piperidino, pyrazolyl, pyrimidinyl, pyridazinyl or piperazinyl. The compounds are mentioned as being central nervous system depressants useful as ataractic agents or tranquilisers, as having antiinflammatory and analgesic properties. The compounds are mentioned as increasing the intracellular concentration of adenosine-3',5'-cyclic monophosphate and for alleviating the symptoms of asthma.

    [0004] H. Hoehn et al., J. Heterocycl. Chem., 1972, 9(2), 235-253 discloses a series of 1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid derivatives with 4-hydroxy, 4-chloro, 4-alkoxy, 4-hydrazino, and 4-amino substituents.

    [0005] CA 1003419, CH 553 799 and T.Denzel, Archiv der Pharmazie, 1974, 307(3), 177-186 disclose 4,5-disubstituted 1H-pyrazolo[3,4-b]pyridines unsubstituted at the 1-position.

    [0006] US 3,833,598 and GB 1,417,489 (E.R.Squibb & Sons) disclose 4-amino derivatives of pyrazolo[3,4-b]pyridine-6-carboxylic acids and esters. The 4-amino group NR3R4 can be an acyclic amino group wherein R3 and R4 may each be hydrogen, lower alkyl, R6,R7-phenyl, etc.; or NR3R4 can be a 5-6-membered heterocyclic group in which an additional nitrogen is present, namely optionally substituted pyrrolidino, piperidino, pyrazolyl, dihydropynidazinyl or piperazinyl. At the 5-position of the pyrazolo[3,4-b]pyridine is group R5 which is hydrogen, lower alkyl, phenyl, phenyl-lower-alkyl or halogen; R5 is preferably hydrogen, methyl or chlorine. The compounds are mentioned as being central nervous system depressants useful as tranquilizers or ataractic agents for the relief of anxiety and tension states. The compounds are also mentioned as increasing the intracellular concentration of adenosine-3',5'-cyclic monophosphate and for alleviating the symptoms of asthma. The compounds are also mentioned as having anti-inflammatory properties and as being useful as anti-inflammatory agents, for example, to reduce local inflammatory conditions such as those of an edematous nature or resulting from proliferation of connective tissue in various mammalian species such as rats and dogs.

    [0007] US 4,115,394 and GB 1,511,006 (E.R.Squibb & Sons) disclose 4-amino derivatives of 6-phenyl-pyrazolo[3,4-b]pyridines. The 4-amino group NR3R4 is an acyclic amino group wherein R3 and R4 may each be hydrogen, lower alkyl, phenyl, phenyl-lower-alkyl or substituted phenyl. At the 5-position of the pyrazolo[3,4-b]pyridine is group R5 which is hydrogen, lower alkyl, phenyl or phenyl-lower-alkyl; R5 is preferably hydrogen. The compounds are mentioned as having anti-inflammatory properties and as being useful as anti-inflammatory agents, for example, to reduce local inflammatory conditions such as those of an edematous nature or resulting from proliferation of connective tissue in various mammalian species such as rats and dogs. The compounds are also mentioned (a) as having diuretic activity, and (b) as increasing the intracellular concentration of adenosine-3',5'-cyclic monophosphate and for alleviating the symptoms of asthma.

    [0008] Japanese laid-open patent application JP-2002-20386-A (Ono Yakuhin Kogyo KK) published on 23 January 2002 discloses pyrazolopyridine compounds of the following formula:

    wherein R1 denotes 1) a group -OR6, 2) a group -SR7, 3) a C2-8 alkynyl group, 4) a nitro group, 5) a cyano group, 6) a C1-8 alkyl group substituted by a hydroxy group or a C1-8 alkoxy group, 7) a phenyl group, 8) a group -C(O)R8, 9) a group -SO2NR9R10, 10) a group -NR11SO2R12, 11) a group -NR13C(O)R14 or 12) a group -CH=NR15. R6 and R7 denote i) a hydrogen atom, ii) a C1-8 alkyl group, iii) a C1-8 alkyl group substituted by a C1-8 alkoxy group, iv) a trihalomethyl group, v) a C3-7 cycloalkyl group, vi) a C1-8 alkyl group substituted by a phenyl group or vii) a 3-15 membered mono-, di- or tricyclic hetero ring containing 1-4 nitrogen atoms, 1-3 oxygen atoms and/or 1-3 sulphur atoms. R2 denotes 1) a hydrogen atom or 2) a C1-8 alkoxy group. R3 denotes 1) a hydrogen atom or 2) a C1-8 alkyl group. R4 denotes 1) a hydrogen atom, 2) a C1-8 alkyl group, 3) a C3-7 cycloalkyl group, 4) a C1-8 alkyl group substituted by a C3-7 cycloalkyl group, 5) a phenyl group which may be substituted by 1-3 halogen atoms or 6) a 3-15 membered mono-, di- or tricyclic hetero ring containing 1-4 nitrogen atoms, 1-3 oxygen atoms and/or 1-3 sulphur atoms. R5 denotes 1) a hydrogen atom, 2) a C1-8 alkyl group, 3) a C3-7 cycloalk-yl group, 4) a C1-8 alkyl group substituted by a C3-7 cycloalkyl group or 5) a phenyl group which may be substituted by 1-3 substituents. In group R3, a hydrogen atom is preferred. In group R4, methyl, ethyl, cyclopropyl, cyclobutyl or cyclopentyl are preferred. The compounds of JP-2002-20386-A are stated as having PDE4 inhibitory activity and as being useful in the prevention and/or treatment of inflammatory diseases and many other diseases.

    [0009] EP 0 076 035 A1 (ICI Americas) discloses pyrazolo[3,4-b]pyridine derivatives as central nervous system depressants useful as tranquilisers or ataractic agents for the relief of anxiety and tension states.

    [0010] The compound cartazolate, ethyl 1-ethyl-4-n-butylamino-1H-pyrazolo[3,4-b]-pyridine-5-carboxylate, is known. J.W. Daly et al., Med. Chem. Res., 1994, 4, 293-306 and D. Shi et al., Drug Development Research, 1997, 42, 41-56 disclose a series of 4-(amino)substituted 1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid derivatives, including ethyl 4-cyclopentylamino-1-methyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate, and their affinities and antagonist activities at A1- and A2A-adenosine receptors, and the latter paper discloses their affinities at various binding sites of the GABAA-receptor channel. S. Schenone et al., Bioorg. Med. Chem. Lett., 2001, 11, 2529-2531, and F. Bondavalli et al., J. Med. Chem., 2002, vol. 45 (Issue 22, 24 October 2002, allegedly published on the Web on 09/24/2002), pp. 4875-4887, disclose a series of 4-amino-1-(2-chloro-2-phenylethyl)-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid ethyl esters as A1-adenosine receptor ligands.

    [0011] WO 02/060900 A2 appears to disclose, as MCP-1 antagonists for treatment of allergic, inflammatory or autoimmune disorders or diseases, a series of bicyclic heterocyclic compounds with a -C(O)-NR4-C(O)-NR5R6 substituent, including isoxazolo[5,4-b]pyridines and 1H-pyrazolo[3,4-b]pyridines (named as pyrazolo[55,4-b]pyridines) with the -C(O)-NR4-C(O)-NR5R6 group as the 5-substituent and optionally substituted at the 1-,3-,4-, and/or 6-positions. Bicyclic heterocyclic compounds with a -C(O)NH2 substituent instead of the -C(O)-NR4-C(O)-NR5R6 substituent are alleged to be disclosed in WO 02/060900 as intermediates in the synthesis of the -C(O)-NR4-C(O)-NR5R6 substituted compounds.

    [0012] S.S.Chakravorti et al., Indian J. Chem., 1978, 16B(2), 161-3 discloses the compounds 4-hydroxy-1,3-diphenyl-5-(3',4'-dihydroisoquinol-1'-yl)-pyrazolo[3,4-b]pyridine and 1,3-diphenyl-4-hydroxy-5-(3'-methyl-3',4'-dihydroisoquinol-1'-yl)-pyrazolo[3,4-b]pyridine. These two compounds were tested for antifilarial activity but were found to have no significant microfilaricidal activity.

    [0013] G. Sabitha et al., Synthetic Commun, 1999, 29(4), 655-665 discloses a synthetic route to 5-substituted-6-amino-1-phenyl-3-(methyl or phenyl)-pyrazolo[3,4-b]pyridines wherein the 5-substituent of the pyrazolo[3,4-b]pyridine is benzimidazol-2-yl, 5-chloro-benzoxazol-2-yl, or benzothiazol-2-yl. Though declared to be "biologically interesting molecules", there is however no disclosure that these compounds had been tested in any pharmacological tests and there is no disclosure of any general or specific biological activity of these compounds.

    [0014] On 8th April 2003, Chemical Abstracts (CAS) registered on their database a compound with the CAS Registry Number 502143-17-1, with the chemical name "1H-Pyrazolo[3,4-b]pyridin-4-amine, N-butyl-5-(4,5-dihydro-1H-imidazol-2- yl)-1-ethyl-" and bearing the laboratory code NSC 235755. As at 5th November 2003, the CAS entry for this compound had no associated literature references and therefore it appears that no chemical synthesis and no uses of the compound have been disclosed as at 5th November 2003. The structure of the compound from the CAS database is as follows:

    WO 00/15222 discloses fused pyridine inhibitors of cGMP phosphodiesterase, e.g. cGrMP PDE V.

    [0015] It is desirable to find new compounds which bind to, and preferably inhibit, phosphodiesterase type IV (PDE4).

    [0016] The present invention provides a compound of formula (I) or a salt thereof (in particular, a pharmaceutically acceptable salt thereof):

    wherein:

    R1 is C1-3alkyl, C1-2fluoroalkyl or -(CH2)2OH;

    R2 is a hydrogen atom (H), methyl or C1fluoroalkyl;

    R3 is optionally substituted C3-8cycloalkyl, or an optionally substituted heterocyclic group of sub-formula (aa), (bb) or (cc):

    in which n1 and n2 independently are 1 or 2; and Y is O, S, SO2, or NR4; where R4 is a hydrogen atom (H), C1-2alkyl, C1-2fluoroalkyl, C(O)NH2, C(O)-C1-2alkyl, or C(O)-C1fluoroalkyl;
    wherein in R3 the C3-8cycloalkyl is optionally substituted with one or two substituents independently being (e.g. being) oxo (=O); OH; C1alkoxy; C1fluoroalkoxy; NHR21 wherein R21 is a hydrogen atom (H); C1-2alkyl; C1fluoroalkyl (such as -CH2F or -CHF2); -CH2OH; -CH2NHR22 wherein R22 is H; -C(O)OR23 wherein R23 is H; -C(O)NHR24 wherein R24 is H; -C(O)R25 wherein R25 is methyl; fluoro; hydroxyimino (=N-OH); or (C1-2alkoxy)imino (=N-OR26 where R26 is C1-2alkyl); and wherein any OH, alkoxy, fluoroalkoxy or NHR21 substituent is not substituted at the R3 ring carbon attached (bonded) to the -NH- group of formula (I);
    and wherein in R3 the heterocyclic group of sub-formula (aa), (bb) or (cc) is optionally substituted with one or two substituents being oxo (=O);
    and R3a is a hydrogen atom (H);
    and wherein Het is of sub-formula (i), (ii), (iii), (iv) or (v):

    wherein:

    W1, W2, W4 and W5 are N; and W3 is NRW;

    X1, X3 and X4 are N or CRX; X2 is O, S or NRX; and X5 is CRX1RX2 or CRX3RX4;

    Y1, Y2 and Y3 are CRY or N; Y4 is O, S or NRY; and Y5 is CRY1RY2;

    Z1 and Z5 are O, S or NRZ; and Z2, Z3 and Z4 are N or CRZ;

    wherein:

    RW is a hydrogen atom (H);

    RX, RX2, RY and RY2 independently are:

    a hydrogen atom (H);

    C1-8alkyl;

    C3-6cycloalkyl optionally substituted by one or two C1-2alkyl groups and/or by one oxo (=O) group;

    -(CH2)n2a-C3-6cycloalkyl optionally substituted, in the -(CH2)n2a- moiety or in the C3-6cycloalkyl moiety, by a C1-2alkyl group, or optionally substituted in the C3-6cycloalkyl moiety by a -CH2C(O)NHC1-2alkyl group, wherein n2a is 1, 2 or 3;

    -(CH2)n3-S(O)2-R5, -CH(C1-2alkyl)-S(O)2-R5, -CMe2-S(O)2-R5, or C3-5cycloalkyl substituted at the connecting carbon atom by -S(O)2-R5, wherein n3 is or 2;
    and R5 is C1-4alkyl (e.g. C1-3alkyl), -NR15R16, phenyl, carbon-linked-pyridinyl or benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two substituents independently being fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy, C1fluoroalkoxy or OH, and wherein the pyridinyl is optionally substituted by one methyl, methoxy or OH (including any tautomer thereof));
    wherein R15 is H, C1-4alkyl (e.g. C1-2alkyl), phenyl, benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two substituents independently being fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-22alkoxy or C1fluoroalkoxy), CH(Me)Ph, or carbon-linked-pyridinyl optionally substituted by one methyl, methoxy or OH (including any tautomer thereof);
    and R16 is H or C1-2alkyl;
    or wherein R15 and R16 together are -(CH2)n3a-X3a-(CH2)n3b- which n3a and n3b independently are 2 or 3 and X3a is a bond, -CH2-, O, or NR8a wherein R8a is H or C1-2alkyl, acetyl, -S(O)2Me or phenyl, and wherein the ring formed by NR15R16 is optionally substituted on a ring carbon by one or two substituents independently being methyl or oxo (=O);

    -(CH2)n4-NR6R7, -CH(C1-2alkyl)-NR6R7, -CMe2-NR6R7, or C3-5cycloalkyl substituted at the connecting carbon atom by -NR6R7, wherein n4 is 0, 1, 2 or 3;
    and R6 and R7 independently are H, C1-6alkyl (e.g. C1-4alkyl), C3-6cycloalkyl, -CH2-C3-6cycloalkyl, -C(O)R17, -S(O)2R18, phenyl, benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two substituents independently being fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy), or carbon-linked-pyridinyl optionally substituted by one methyl, methoxy or OH (including any tautomer thereof);
    and wherein R17 and R18 independently are C1-6alkyl (e.g. C1-4alkyl or C1-2alkyl or isopropyl or n-propyl), C3-6cycloalkyl, optionally substituted 5-membered heteroaryl being furyl (furanyl, e.g. 2-furyl) or 1,3-oxazolyl or isoxazolyl or oxadiazolyl or thienyl (e.g. 2- or 3- thienyl) or 1,3-thiazolyl or isothiazolyl or pyrrolyl or imidazolyl or pyrazolyl (all independently optionally substituted by one oxo and/or one or two methyl), or phenyl or benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two substituents independently being fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy, C1fluoroalkoxy or OH), or carbon-linked-pyridinyl optionally substituted by one methyl, methoxy or OH (including any tautomer thereof);
    or R6 and R7 together are -(CH2)n5-X5-(CH2)n6- in which n5 and n6 independently are 2 or 3 and X5 is a bond, -CH2-, O, or NR8 wherein R8 is H, C1-2alkyl, acetyl, -S(O)2Me or phenyl, and wherein the ring formed by NR6R7 is optionally substituted on a ring carbon by one or two substituents independently being methyl or oxo (=O);

    -(CH2)n7-O-R9; wherein n7 is 0, 1, 2 or 3 and R9 is H, C1-6akyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, -C(O)R17, phenyl, or benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy); wherein n7 is 0 only when the -(CH2)n7-O-R9 is bonded to a carbon atom in the Het ring; and wherein n7 is not 0 when Het is of sub-formula (v) (i.e. n7 is not 0 for RX2 and for RY2);

    -(CH2)n11-C(O)-NR10R11, -CH(C1-2akyl)-C(O)-NR10R11, -CMe2-C(O)-NR10R11, or C3-5cycloalkyl substituted at the connecting carbon atom by -C(O)-NR10R11, wherein n11 is 0, 1 or 2; and wherein R10 and R11 independently are: H; C1-6alkyl; C1-4fluoroalkyl; C2-4alkyl substituted by one OH or -OC1-2alkyl other than at the connection point; C3-6cycloalkyl optionally substituted by one or two methyl groups; -CH2-C3-6cycloalkyl optionally substituted by one methyl, NH2 or NHMe group; -(CH2)n17-Het2; carbon-linked-pyridinyl optionally substituted by one methyl, methoxy or OH (including any tautomer thereof); phenyl; benzyl; or -CH(C1-2alkyl)Ph [wherein the phenyl, benzyl, and -CH(C1-2alkyl)Ph are independently optionally substituted on the aromatic ring by one or two substituents independently being: fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy, C1fluoroalkoxy, OH, -NR10aR10b (wherein R10a is H or C1-2alkyl and R10b is H, C1-2alkyl, -C(O)-C1-2alkyl or -S(O)2-C1-2alkyl), -C(O)-NR10cR10d (wherein R10c and R10d independently are H or C1-2alkyl), or -S(O)2-R10e (wherein R11e is C1-2alkyl, NH2, NHMe or NMe2)],
    wherein n17 is 0, 1 or 2 and wherein Het2 is a 4-, 5- or 6- membered saturated heterocyclic ring containing one O or S ring atom or one NR27 ring group wherein R27 is H, C1-2alkyl, -C(O)Me, or -S(O)2Me, wherein the Het2 ring is optionally substituted on a ring carbon by one or two substituents independently being methyl or oxo (=O);
    and wherein when n17 is 2 then the Het2 ring can optionally contain one additional ring N atom at the Het2 ring position bonded to the -(CH2)n17-moiety; provided that, when Het2 contains one O or S or NR27 ring atom/group and one additional ring N atom, then the O/S/NR27 ring atom/group and the one additional ring N atom are not directly bonded to each other, and are separated by more than one carbon atom;
    or R10 and R11 together are -(CH2)n8-X6-(CH2)n9- in which n8 and n9 independently are 2 or 3 and X6 is a bond, -CH2-, O, or NR12 wherein R12 is H, C1-2alkyl, acetyl, -S(O)2Me or phenyl, and wherein the ring formed by NR10R11 is optionally substituted on a ring carbon by one or two substituents independently being methyl or oxo (=O);

    -(CH2)n12-C(O)-OR13 wherein n12 is 0, 1 or 2; and wherein R13 is H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, phenyl, or benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two of (independently) fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy);

    -(CH2)n13-C(O)-R13a wherein n13 is 0, 1 or 2; and wherein R13a is a hydrogen atom (H), C1-6akyl, C1-2fluoroalkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, benzyl, or phenyl; wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two of (independently) fluoro, chloro, C1-2akyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy;

    -(CH2)n14-Het1, -CH(C1-2alkyl)-Het1, -CMe2-Het1, or C3-5cycloalkyl substituted at the connecting carbon atom by Het1, wherein n14 is 0, 1 or 2 and wherein Het1 is a 4-, 5-, 6- or 7-membered saturated heterocyclic ring;

    wherein said heterocyclic ring Het1 contains one O or S ring atom and/or one NR14 ring group wherein R14 is H, C1-4akyl, C3-6cycloalkyl, benzyl, phenyl, -C(O)R19, or -S(O)2R19;
    wherein R19, independent of any other R19, is C1-6alkyl (e.g. C1-4alkyl or C1-3alkyl), C3-6cycloalkyl, thienyl (e.g. 2-thienyl), furyl (furanyl, e.g. furan-2-yl), or phenyl or benzyl; wherein the phenyl and benzyl are independently optionally substituted by one or two of (independently) fluoro, methyl or methoxy;
    and wherein said heterocyclic ring Het1 is optionally substituted (at a position or positions other than any NR14 position) by one or two oxo (=O) and/or one C1-4alkyl substituents;
    provided that, when the heterocyclic ring Het1 contains one O or S ring atom and one NR14 ring group then: (a) the O/S ring atom and the NR14 ring group are not directly bonded to each other, and (b) the O/S ring atom and the NR14 ring group are separated by more than one carbon atom unless Het1 contains an -NR14-C(O)-O- or -NR14-C(O)-s- moiety as part of the ring; or
    • (CH2)n10-Ar, -CH(C1-2alkyl)-Ar, -CMe2-Ar, or C3-5cycloalkyl substituted at the connecting carbon atom by Ar, wherein n10 is 0, 1 or 2 and

      (i) Ar is phenyl optionally substituted by one or two substituents independently being fluoro, chloro, bromo, C1-2alkyl, C1-2fluoroalkyl, C1-2alkoxy, C1-2fluoroalkoxy, OH, -NR11aR11b (wherein R11a is H or C1-2alkyl and R11b is H, C1-2alkyl, -C(O)-C1-2alkyl or -S(O)2-C1-2alkyl), cyano, -C(O)-NR11cR11d (wherein R11c and R11d independently are H or C1-2alkyl), -C(O)-OR11e wherein R11e is H or C1-2alkyl, or -S(O)2-R11f (wherein R11f is C1-2alkyl, NH2, NHMe or NMc2); or the phenyl Ar is optionally substituted at two adj acent Ar ring atoms by the two ends of a chain which is: -(CH2)4-, -(CH2)3-, or -CH=CH-CH=CH-; or

      (ii) Ar is an optionally substituted 5- or 6-membered heterocyclic aromatic ring containing 1, 2, 3 or 4 heteroatoms (e.g. 1, 2 or 3 heteroatoms) selected from O, N or S; and wherein when the heterocyclic aromatic ring Ar contains 2, 3 or 4 heteroatoms (e.g. 2 or 3 heteroatoms), one is selected from O, N and S and the remaining heteroatom(s) are N; and wherein the heterocyclic aromatic ring Ar is optionally substituted by one or two groups independently being C1-4alkyl (e.g. C1-2alkyl) or OH (including any keto tautomer of an OH-substituted aromatic ring), or the heterocyclic aromatic ring Ar is optionally substituted at two adjacent Ar ring atoms by the two ends of a chain which is: -(CH2)4-, -(CH2)3-, or -CH=CH-CH=CH-;

    RX1 and RY1 independently are a hydrogen atom (H), C1-2alkyl or C1fluoroalkyl;

    RX3 and RX4 together are -(CH2)n15-X7-(CH2)n16- wherein n15 and n16 independently are 1 or 2 and X7 is a bond, -CH2-, O, or NRX5 wherein RX5 is H, C1-2alkyl, acetyl or -S(O)2Me; and

    RZ is a hydrogen atom (H);

    provided that:

    when R3 is the heterocyclic group of sub-formula (bb), n1 is 1, and Y is NR4, then R4 is not C1-2alkyl or C1-2fluoroalkyl;

    when R3 is the heterocyclic group of sub-formula (aa) and Y is NR4, then R4 is not C(O)NH2, C(O)-C1-2alkyl or C(O)-C1fluoroalkyl;

    provided that: when R3 is the heterocyclic group of sub-formula (cc), then NR3R3a is of sub-formula (L), (m), (m1) or (m2), wherein the -NH- connection point of the NR3R3a group to the 4-position of the pyrazolopyridine of formula (I) is underlined:

    and provided that:

    where R3 is optionally substituted C3-8cycloalkyl, then any OH, alkoxy, fluoroalkoxy, -CH2OH, -CH2NHR22, -C(O)OR23, -C(O)NHR24, -C(O)R25 or fluoro substituent is at the 3-, 4- or 5-position of the R3 cycloalkyl ring (wherein, in this connection, the 1-position of the R3 cycloalkyl ring is deemed to be the connection point to the -NH- in formula (I)).
    In one optional embodiment of the invention, R3 is optionally substituted C3-8cycloalkyl, or an optionally substituted heterocyclic group of sub-formula (aa), (bb) or (cc):

    in which n1 and n2 are 1 or 2; and Y is O, S, SO2, or NR4; where R4 is a hydrogen atom, C1-2alkyl, C1-2fluoroalkyl, C(O)NH2, C(O)-C1-2alkyl, or C(O)-C1fluoroalkyl; provided that Y is not NR4 when the heterocyclic group is of sub-formula (aa).

    [0017] Alternatively or additionally, in one optional embodiment of the invention, in R3 the C3-8cycloalkyl is optionally substituted with one or two substituents being oxo (=O), OH, C1alkoxy, C1fluoroalkoxy, or C1-2alkyl; and wherein any OH, alkoxy or fluoroalkoxy substituent is not substituted at the R3 ring carbon attached to the -NH-group of formula (1).

    [0018] Alternatively or additionally, in one optional embodiment of the invention, Het is of sub-formula (i), (ii), (iii) or (iv):

    wherein:

    W1, W2 and W4 are N; and W3 is NRW;

    X1, X3 and X4 are N or CRX; and X2 is O, S or NRX;

    Y1, Y2 and Y3 are CRY or N; and Y4 is O S or NRY;

    Z1 is O, S or NRZ; and Z2, Z3 and Z4 are N or CRZ;

    and wherein:

    RW is a hydrogen atom (H); and

    RZ is a hydrogen atom (H).



    [0019] Alternatively or additionally, in one optional embodiment of the invention, RX and RY independently are:

    a hydrogen atom (H);

    C1-8alkyl;

    C3-6cycloalkyl;

    -(CH2)n3-SO2-R5 wherein n3 is 1 or 2 and R5 is C1-3alkyl or NH-C1-2alkyl;

    -(CH2)n4-NR6R7 wherein n4 is 0, 1 or 2, and R6 and R7 independently are H, C1-6alkyl e.g. C1-4alkyl, -C(O)-C1-2alkyl or -SO2-C1-2alkyl; or R6 and R7 together are -(CH2)n-5-X5-(CH2)n6- in which n5 and n6 independently are 2 or 3 and X5 is a bond, -CH2-, O, or NR8 wherein R8 is H or C1-2alkyl;

    -(CH2)n7-O-R9 wherein n7 is 1 or 2 and R9 is H or C1-6alkyl;

    -C(O)-NR10R11 wherein R10 and R11 independently are H or C1-6alkyl; or R10 and R11 together are -(CH2)n8-X6-(CH2)n9- in which n8 and n9 independently are 2 or 3 and X6 is a bond, -CH2-, O, or NR12 wherein R12 is H or C1-2alkyl;

    -C(O)-OR13 wherein R13 is H or C1-6alkyl;

    a 4-, 5-, 6- or 7-membered saturated heterocyclic ring containing one O ring atom or one NR14 ring group wherein R14 is H or C1-4alkyl, said heterocyclic ring being optionally substituted (at a position or positions other than any NR14 position) by one oxo (=O) and/or one C1-4alkyl substituent; or

    -(CH2)n10-Ar wherein n10 is 0, 1 or 2 and

    1. (i) Ar is phenyl optionally substituted by one or two substituents being fluoro, chloro, C1-2alkyl, C1-2fluoroalkyl, C1-2alkoxy, C1-2fluoroalkoxy or cyano; or
    2. (ii) Ar is an optionally substituted 5- or 6-membered heterocyclic aromatic ring containing 1, 2 or 3 heteroatoms selected from O, N or S; and wherein when the heterocyclic aromatic ring Ar contains 2 or 3 heteroatoms, one is selected from O, N and S and the remaining heteroatom(s) are N; and wherein the heterocyclic aromatic ring Ar is optionally substituted by one or two C1-4alkyl groups.



    [0020] Alternatively or additionally, in one optional embodiment of the invention, Het is of sub-formula (i), (ii), (iii), (iv) or (v):

    wherein:

    W1, W2, W4 and W5 are N; and W3 is NRW;

    X1, X3 and X4 are N or CRX; X2 is O, S or NRX ; and X5 is CRX1RX2;

    Y1, Y2 and Y3 are CRY or N; Y4 is O, S or NRY ; and Y5 is CRY1RY2;

    Z1 and Z5 are O, S or NRZ; and Z2, Z3 and Z4 are N or CRZ;

    and wherein:

    RW is a hydrogen atom (H); and

    RZ is a hydrogen atom (H).



    [0021] In one optional embodiment of the invention, RX, RX2, RY and RY2 independently are, or RX and RY independently are:

    a hydrogen atom (H);

    C1-8alkyl;

    C3-6cycloalkyl optionally substituted by a C1-2alkyl group;

    -(CH2)n2a-C3-6cycloalkyl optionally substituted, in the -(CH2)n2a- moiety or in the C3-6cycloalkyl moiety, by a C1-2alkyl group, wherein n2a is 1, 2 or 3;

    -(CH2)n3-SO2-R5 wherein n3 is 1 or 2 and R5 is C1-3alkyl or -NH-C1-2alkyl or phenyl;

    -(CH2)n4-NR6R7 wherein n4 is 0, 1, 2 or 3, and R6 and R7 independently are H, C1-6alkyl e.g. C1-4alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl,

    -C(O)-C1-2alkyl, -SO2-C1-2alkyl, phenyl, or benzyl (wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy); or R6 and R7 together are -(CH2)n5-X5-(CH2)n6- in which n5 and n6 independently are 2 or 3 and X5 is a bond, -CH2-, O, or NR8 wherein R8 is H or C1-2alkyl;

    -(CH2)n7-O-R9; wherein n7 is 0, 1, 2 or 3 and R9 is H or C1-6alkyl; wherein n7 is 0 only when the -(CH2)n7-O-R9 is bonded to a carbon atom in the Het ring; and wherein n7 is not 0 when Het is of sub-formula (v) (i.e. n7 is not 0 for RX2 and for RY2);

    -C(O)-NR10R11 wherein R10 and R11 independently are H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, phenyl, or benzyl (wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy); or R10 and R11 together are -(CH2)n8-X6-(CH2)n9- in which n8 and n9 independently are 2 or 3 and X6 is a bond, -CH2-, O, or NR12 wherein R12 is H or C1-2alkyl;

    -C(O)-OR13 wherein R13 is H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cyloalkyl, phenyl, or benzyl (wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy);

    -C(O)-R13a wherein R13a is a hydrogen atom (H), C1-6alkyl, C1-2fluoroalkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, benzyl, or phenyl; wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy;

    a 4-, 5-, 6- or 7-membered saturated heterocyclic ring containing one O ring atom or one NR14 ring group wherein R14 is H or C1-4alkyl, said heterocyclic ring being optionally substituted (at a position or positions other than any NR14 position) by one oxo (=O) and/or one C1-4alkyl substituent; or

    -(CH2)n10-Ar wherein n10 is 0, 1 or 2 and

    1. (i) Ar is phenyl optionally substituted by one or two substituents being fluoro, chloro, C1-2alkyl, C1-2fluoroalkyl, C1-2alkoxy, C1-2fluoroalkoxy or cyano;
      or
    2. (ii) Ar is an optionally substituted 5- or 6-membered heterocyclic aromatic ring containing 1, 2 or 3 heteroatoms selected from O, N or S; and wherein when the heterocyclic aromatic ring Ar contains 2 or 3 heteroatoms, one is selected from O, N and S and the remaining heteroatom(s) are N; and wherein the heterocyclic aromatic ring Ar is optionally substituted by one or two C1-4alkyl groups; and

    RX1 and RY1 independently are a hydrogen atom (H), C1-2alkyl or C1fluoroalkyl.



    [0022] In compounds, for example in the compounds of formula (I), an "alkyl" group or moiety may be straight-chain or branched. Alkyl groups, for example C1-8alkyl or C1-6alkyl or C1-4alkyl or C1-3alkyl or C1-2alkyl, which may be employed include C1-6alkyl or C1-4alkyl or C1-3alkyl or C1-2alkyl such as methyl, ethyl, n-propyl, n-butyl, n-pentyl, or n-hexyl, or any branched isomers thereof such as isopropyl, t-butyl, sec-butyl, isobutyl, 3-methylbutan-2-yl, 2-ethylbutan-1-yl, or the like.

    [0023] A corresponding meaning is intended for "alkoxy", "alkylene", and like terms derived from alkyl. For example, "alkoxy" such as C1-6alkoxy or C1-4alkoxy or C1-2alkoxy includes methoxy, ethoxy, propyloxy, and oxy derivatives of the alkyls listed above. "Alkylsulfonyl" such as C1-4alkylsulfonyl includes methylsulfonyl (methanesulfonyl), ethylsulfonyl, and others derived from the alkyls listed above. "Alkylsulfonyloxy" such as C1-4alkylsulfonyloxy includes methanesulfonyloxy (methylsulfonyloxy), ethanesulfonyloxy, et al.

    [0024] "Cycloalkyl", for example C3-8cycloalkyl, includes cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, and the like. Preferably, a C3-8 cycloalkyl group is C3-6cycloalkyl or C5-6cyeloalkyl, that is the cycloalkyl group contains a 3-6 membered or 5-6 membered carbocyclic ring respectively.

    [0025] "Fluoroalkyl" includes alkyl groups with one, two, three, four, five or more fluorine substituents, for example C1-4fluoroalkyl or C1-3fluoroalkyl or C1-2fluoroalkyl such as monofluoromethyl, difluoromethyl, trifluoromethyl, pentafluoroethyl, 2,2,2-trifluoroethyl (CF3CH2-), 2,2-difluoroethyl (CHF2-CH2-), or 2-fluoroethyl (CH2F-CH2-), etc. "Fluoroalkoxy" includes C1-4fluoroalkoxy or C1-2fluoroalkoxy such as trifluoromethoxy, pentafluoroethoxy, monofluoromethoxy, difluoromethoxy, etc. "Fluoroalkylsulfonyl" such as C1-4fluoroalkylsulfonyl includes trifluoromethanesulfonyl, pentafluoroethylsulfonyl, etc.

    [0026] A halogen atom ("halo") present in compounds, for example in the compounds of formula (I), means a fluorine, chlorine, bromine or iodine atom ("fluoro", "chloro", "bromo" or "iodo").

    [0027] When the specification states that atom or moiety A is "bonded" or "attached" to atom or moiety B, it means that atom/moiety A is directly bonded to atom/moiety B usually by means of one or more covalent bonds, and excludes A being indirectly attached to B via one or more intermediate atoms/moieties (e.g. excludes A-C-B); unless it is clear from the context that another meaning is intended.

    [0028] By "carbon-linked-pyridinyl" is meant pyridin-2-yl, pyridin-3-yl, or pyridin-4-yl. R1 is C1-3alkyl, C1-2fluoroalkyl or -(CH2)2OH. R1 is preferably C2-3alkyl, C2fluoroalkyl or -(CH2)2OH; and more preferably C2alkyl or C2fluoroalkyl. When R1 is C1-3alkyl, it can be straight-chained or branched. R1 can for example be methyl, trifluoromethyl, ethyl, n-propyl, isopropyl, C2fluoroalkyl or -(CH2)2OH; and more preferably R1 is ethyl, n-propyl, C2fluoroalkyl (e.g. C1fluoroalkyl-CH2- such as CF3-CH2-) or -(CH2)2OH. R1 is most preferably ethyl.

    [0029] Preferably, R2 is a hydrogen atom (H) or methyl, more preferably a hydrogen atom (H).

    [0030] R3 is optionally substituted C3-8cycloalkyl, or an optionally substituted heterocyclic group of sub-formula (aa), (bb) or (cc).

    [0031] Preferably, in R3 there is one substituent or no substituent.

    [0032] In one optional embodiment, where R3 is optionally substituted C3-8cycloalkyl, it is not optionally substituted C5cycloalkyl, i.e. not optionally substituted cyclopentyl. In this case, more preferably, R3 is optionally substituted C6-8cycloalkyl or optionally substituted C6-7cycloalkyl.

    [0033] Where R3 is optionally substituted C3-8cycloalkyl, it is more preferably optionally substituted C6cycloalkyl (i.e. optionally substituted cyclohexyl); for example C6cycloalkyl optionally substituted with one or two substituents independently being (e.g. being) oxo (=O), OH, C1alkoxy, C1fluoroalkoxy (e.g. trifluoromethoxy), or C1-2alkyl, and wherein any OH, alkoxy or fluoroalkoxy substituent is not substituted at the R3 ring carbon attached (bonded) to the -NH- group of formula (I).

    [0034] Where R3 is optionally substituted C3-8cycloalkyl, the one or two optional substituents is or independently are (e.g. is or are) oxo (=O); OH; C1alkoxy; C1fluoroalkoxy (e.g. trifluoromethoxy); NHR21 wherein R21 is a hydrogen atom (H); C1-2alkyl such as methyl; C1fluoroalkyl such as -CH2F or -CHF2; -CH2OH; -CH2NHR22 wherein R22 is H; -C(O)OR23 wherein R23 is H; -C(O)NHR24 wherein R24 is H; -C(O)R25 wherein R25 is methyl; fluoro; hydroxyimino (=N-OH); or (C1-2alkoxy)imino (=N-OR26 where R26 is C1-2alkyl); and wherein any OH, alkoxy, fluoroalkoxy or NHR21 substituent is not substituted at the R3 ring carbon attached (bonded) to the -NH- group of formula (I).

    [0035] Preferably, where R3 is optionally substituted C3-8cycloalkyl, the one or two optional substituents comprise (e.g. is or independently are (e.g. is or are)) oxo (=O); OH; NHR21 wherein R21 is a hydrogen atom (H); C1-2alkyl such as methyl; C1fluoroalkyl such as -CH2F or -CHF2; -C(O)OR23 wherein R23 is H; -C(O)NHR24 wherein R24 is H; fluoro; hydroxyimino (=N-OH); or (C1-2alkoxy)imino (=N-OR26 where R26 is C1-2alkyl).

    [0036] More preferably, where R3 is optionally substituted C3-8cycloalkyl, the one or two optional substituents comprise (e.g. is or independently are (e.g. is or are)) oxo (=O); OH; NHR21 wherein R21 is a hydrogen atom (H); methyl; -CH2F; -CHF2; -C(O)OR23 wherein R23 is H; fluoro; hydroxyimino (=N-OH); or (C1-2alkoxy)imino (=N-OR26 where R26 is C1-2alkyl). Still more preferably, where R3 is optionally substituted C3-8cycloalkyl, the one or two optional substituents comprise (e.g. is or independently are (e.g. is or are)) oxo (=O); OH; methyl; fluoro; hydroxyimino (=N-OH); or (C1-2alkoxy)imino (=N-OR26 where R26 is C1-2alkyl).

    [0037] Most preferably, where R3 is optionally substituted C3-8cycloalkyl, the one or two optional substituents comprise (e.g. is or independently are (e.g. is or are)) OH, oxo (=O) or hydroxyimino (=N-OH). For example, where R3 is optionally substituted C3-8cycloalkyl, the one or two optional substituents preferably comprise (e.g. is or are) OH and/or oxo (=O).

    [0038] Optionally, in R3, the C3-8cycloalkyl is unsubstituted.

    [0039] Where R3 is optionally substituted C3-8cycloalkyl, e.g. optionally substituted C5-8cycloalkyl such as optionally substituted C6cycloalkyl (optionally substituted cyclohexyl), the one or two optional substituents if present preferably comprise a substituent (for example is or are substituent(s)) at the 3-, 4- or 5- position(s) of the R3 cycloalkyl ring. (In this connection, the 1 -position of the R3 cycloalkyl ring is deemed to be the connection point to the -NH- in formula (I)).

    [0040] Where R3 is optionally substituted C3-8cycloalkyl, any OH, alkoxy, fluoroalkoxy, -CH2OH, -CH2NHR22, -C(O)OR23, -C(O)NHR24, -C(O)R25 or fluoro substituent (particularly any OH substituent) is at the 3-, 4- or 5-position, e.g. the 3- or 5-position, of the R3 cycloalkyl (e.g. C6-8cycloalkyl) ring. For example, any OH, alkoxy, fluoroalkoxy, -CH2OH, -CH2NHR22, -C(O)OR23, -C(O)NHR24, -C(O)R25 or fluoro substituent (particularly any OH substituent) can be at the 3-position of a R3 C5cycloalkyl (cyclopentyl) ring or at the 3-, 4- or 5- position, e.g. 3- or 5-position, of a R3 C6cycloalkyl (cyclohexyl) ring. (In this connection, and also below, the 1-position of the R3 cycloalkyl ring is deemed to be the connection point to the -NH- in formula (I)).

    [0041] Where R3 is optionally substituted C3-8cycloalkyl, any NHR21 substituent is preferably at the 2-, 3-, 4- or 5- position, preferably the 2- or 3-position or more preferably the 3-position, of the R3 cycloalkyl (e.g. C6-8cycloalkyl e.g. cyclohexyl) ring.

    [0042] Where R3 is optionally substituted C3-8cycloalkyl, any alkyl or fluoroalkyl substituent is preferably at the 1-, 2-, 3-, 4- or 5- position, more preferably the 1-, 2-, 3- or 5-position, still more preferably the 1- or 3-position, of the R3 cycloalkyl (e.g. C6-8cycloalkyl e.g. cyclohexyl) ring.

    [0043] Where R3 is optionally substituted C3-8cycloalkyl, any oxo (=O), hydroxyimino (=N-OH); or (C1-4alkoxy)imino (=N-OR26) substituent is preferably at the 3- or 4-position, preferably at the 4-position, of the R3 cycloalkyl (e.g. C6-8cycloalkyl e.g. cyclohexyl) ring.

    [0044] Where R3 is optionally substituted C3-8cycloalkyl, R3 is preferably cyclohexyl (i.e. unsubstituted) or cyclohexyl substituted by one oxo (=O), OH, NHR21, C1-2alkyl, C1fluoroalkyl, -CH2OH, -C(O)OR23, -C(O)NHR24, -C(O)R25, fluoro, hydroxyimino (=N-OH) or (C1-2alkoxy)imino (=N-OR26) substituent, or cyclohexyl substituted by two fluoro substituents. More preferably, R3 is cyclohexyl (i.e. unsubstituted), or cyclohexyl substituted by one oxo (=O), OH, NHR21, C1-2alkyl, C1fluoroalkyl, -C(O)OR23, fluoro, hydroxyimino (=N-OH) or (C1-2alkoxy)imino (=N-OR26) substituent, or cyclohexyl substituted by two fluoro substituents. Still more preferably R3 is cyclohexyl (i.e. unsubstituted) or cyclohexyl substituted by one oxo (=O), hydroxyimino (=N-OH), C1-2alkyl or OH substituent; for example R3 can be cyclohexyl (i.e. unsubstituted) or cyclohexyl substituted by one oxo (=O) or OH substituent. The optional substituent can be at the 3- or 4- position, e.g. 3-position, of the R3 cyclohexyl ring; more preferably any OH substituent is preferably at the 3-position of the R3 cyclohexyl ring, and/or any oxo (=O), hydroxyimino (=N-OH) or (C1-2alkoxy)imino (=N-OR26) substituent is preferably at the 4-position of the R3 cyclohexyl ring.

    [0045] Where R3 is optionally substituted C6cycloalkyl, R3 can for example be 4-hydroxy-cyclohexyl (i.e. 4-hydroxycyclohexan-1-yl) or 3-oxo-cyclohexyl, but R3 is more preferably cyclohexyl (i.e. unsubstituted), 3-hydroxy-cyclohexyl (i.e. 3-hydroxycyclohexan-1-yl), 4-oxo-cyclohexyl (i.e. 4-oxocyclohexan-1-yl), 4-(hydroxyimino)yclohexyl (i.e. 4-(hydroxyimino)cyclohexan-1-yl), 4-(C1-2alkoxyimino)cyclohexyl, 1-methylcyclohexyl or 3-methylcyclohexyl. In one embodiment, R3 can optionally be cyclohexyl (i.e. unsubstituted) or 3-hydroxy-cyclohexyl or 4-oxo-cyclohexyl. Where R3 is optionally substituted C6cycloalkyl, R3 is most preferably cyclohexyl (i.e. unsubstituted), 4-oxo-cyclohexyl (i.e. 4-oxocyclohexan-1-yl) or 4-(hydroxyimino)cyclohexyl (i.e. 4-(hydroxyimino)cyclohexan-1-yl).

    [0046] Where R3 is optionally substituted C5cycloalkyl (optionally substituted cyclopentyl), R3 can for example be cyclopentyl (i.e. unsubstituted) or 3-hydroxy-cyclopentyl.

    [0047] Where R3 is the heterocyclic group of sub-formula (aa), (bb) or (cc), then Y is preferably O, S, SO2, NH or N-C(O)-Me (for example O, S, SO2 or N-C(O)-Me), more preferably O, NH or N-C(O)-Me, still more preferably O or N-C(O)-Me, most preferably O. (When Y is NH or N-C(O)-Me, then R4 is H or -C(O)-Me).

    [0048] Preferably, R4 is a hydrogen atom (H), C1-2alkyl, C(O)NH2, C(O)-Me or C(O)-CF3. Optionally, R4 can be a hydrogen atom (H), C1-2alkyl, C(O)-Me or C(O)-CF3, more preferably H, C(O)-Me or C(O)-CF3, still more preferably H or C(O)-Me.

    [0049] Y is not N-C(O)-Me when the heterocyclic group is of sub-formula (aa).

    [0050] Where R3 is the heterocyclic group of sub-formula (aa), (bb) or (cc), then it is preferable that R3 is the heterocyclic group of sub-formula (aa) or (bb). More preferably, in R3, the heterocyclic group is of sub-formula (bb).

    [0051] In sub-formula (bb), n1 is preferably 1. In sub-formula (cc), n2 is preferably 1. That is, six-membered rings are preferred in the R3 heterocyclic group.

    [0052] Preferably, in R3, the heterocyclic group of sub-formula (aa), (bb) or (cc) is unsubstituted. (In this connection, where Y is NR4, R4 is not classified as a substituent).

    [0053] In the R3 heterocyclic group of sub-formula (aa), (bb) or (cc), the one or two optional substituents is or are oxo (=O). In the R3 heterocyclic group of sub-formula (aa), (bb) or (cc), any oxo (=O) substituents are preferably on a carbon atom bonded (adjacent) to X, and/or can be at the 2-, 3-, 4- or 5- position(s) of the R3 heterocyclic ring. (In this connection, the 1-position of the R3 heterocyclic ring is deemed to be the connection point to the -NH- in formula (I)). Preferably, only oxo (=O) substitution or no substitution is allowed at each of the 2- and 6-positions of the R3 heterocyclic ring.

    [0054] When R3 is the heterocyclic group of sub-formula (aa) and Y is NR4, then R4 is not C(O)R, i.e. R4 is not C(O)NH2, C(O)-C1-2alkyl or C(O)-C1fluoroalkyl. In one embodiment, Y is O, S, SO2 or NH when R3 is the heterocyclic group of sub-formula (aa).

    [0055] When R3 is the heterocyclic group of sub-formula (aa), preferably Y is not NR4.

    [0056] NHR3 or NR3R3a is not

    When R3 is the heterocyclic group of sub-formula (bb) and Y is NR4, and in particular when n1 is 1, then R4 is preferably not alkyl or substituted alkyl, i.e. R4 is preferably not C1-2alkyl or C1-2fluoroalkyl. In one embodiment, when R3 is the heterocyclic group of sub-formula (bb), Y is preferably O, S, SO2 or NR4, wherein R4 is H, C(O)NH2, C(O)-C1-2alkyl or C(O)-C1fluoroalkyl or more preferably H or C(O)-Me. More preferably, for sub-formula (bb), Y is O or NR4.

    [0057] Preferably, NHR3 or NR3R3a is of sub-formula (a), (a1), (b), (c), (c 1), (c 3), (c 4), (c 5), (d), (e), (f), (g), (g1), (g2), (g3), (g4). (h), (i), (j), (k), (k1), (L), (m), (m1), (m2), (m3), (m5), (n), (o), (o1), (o2), (o3), (o4), (p), (p2), (p3), (p5), (p7) or (q):















    [0058] In the sub-formulae (a) to (q) etc above, the -NH- connection point of the NHR3 or NR3R3a group to the 4-position of the pyrazolopyridine of formula (I) is underlined. Generally, in this specification, for a group or radical, where NH or N are underlined, then this indicates the connection point.

    [0059] Preferably, NHR3 or NR3R3a is of sub-formula (c), (c 1), (c 3), (c 4), (c 5), (d), (e), (f), (g1), (g4), (h), (i), (j), (k), (k1), (L), (m), (m1) (m2), (m3), (m5), (n), (o), (o1), (o2), (o3), (o4), (p), (p2), (p3), (p5), (p7) or (q). More preferably, NHR3 or NR3R3a is of sub-formula (c), (c1), (c4), (c5), (h), (i), (j), (k), (m1), (m2), (n), (o), (o2), (o3), (p2) or (p5). Still more preferably, NHR3 or NR3R3a is of sub-formula (c), (h), (k), (n), (o) or (o2); for example (c), (h), (o) or (o2). Most preferably, R3 is tetrahydro-2H-pyran-4-yl; that is NHR3 or NR3R3a is most preferably of sub-formula (h), as shown above.

    [0060] In one embodiment of the invention, NHR3 or NR3R3a is of sub-formula (a), (b), (c), (d), (e), (f), (g), (h), (i), (j), (k), (L), (m), (n), (o), (p) or (q). In this embodiment, NHR3 or NR3R3a is preferably of sub-formula (c), (h), (k), (n) or (o), still more preferably (c), (h), (k), (n) or (o).

    [0061] In another embodiment of the invention, NHR3 or NR3R3a is of sub-formula (a), (b), (c), (d), (e), (f), (g), (g1), (g2), (g3), (h), (i), (j), (k), (L), (m), (m1), (n), (o), (o1), (p) or (q). In this embodiment, preferably, NHR3 or NR3R3a is of sub-formula (c), (d), (e), (f), (h), (g1), (i), (j), (k), (m), (m1), (n), (o), (o1), (p) or (q). More preferably NHR3 or NR3R3a is of sub-formula (c), (h), (k), (n) or (o), still more preferably (c), (h), (k), (n) or (o). Most preferably, R3 is tetrahydro-2H-pyran-4-yl; that is NHR3 or NR3R3a is most preferably of sub-formula (h), shown above.

    [0062] When NHR3 or NR3R3a is of sub-formula (n), then preferably it is a cis-(3-hydroxycyclohex-1-yl)amino group, eg in any enantiomeric form or mixture of forms but it can be racemic.

    [0063] Preferably, Het is of sub-formula (i), (ii), (iii) or (v); more preferably Het is of sub-formula (i), (ii), or (v); still more preferably Het is of sub-formula (i).

    [0064] X1, X3 and/or X4 independently is/are often N (a nitrogen atom).

    [0065] Y1, Y2 and/or Y3 independently is/are often CRY.

    [0066] Suitably, Z1 and/or Z5 independently is/are O or S. Preferably, Z1 and/or Z5 is O.

    [0067] Preferably, Het is of sub-formula (ia), (ib), (ic), (id), (ie), (if) or (ig); more preferably of sub-formula (ia), (ib), (ic), (id), (if) or (ig) or of sub-formula (ia), (ib), (ic), (id), or (ie); still more preferably of sub-formula (ia), (ib), (ic), or (id); yet more preferably of sub-formula (ia), (ic), or (id):





    [0068] Alternatively, when Het is of sub-formula (v), Het can for example be of sub-formula (va) or (vb), more preferably of sub-formula (va):



    [0069] Alternatively, when Het is of sub-formula (ii), Het can for example be of sub-formula (iia):



    [0070] Preferably, Het is of sub-formula (ia), (ib), (ic), (id), (if), (ig), (va) or (iia). More preferably, Het is of sub-formula (ia), (ic), (id) or (va).

    [0071] For the Het group in general, RW and RZ are a hydrogen atom (H).

    [0072] For the Het group in general, preferably, one of RX and RY (or RX2 and RY2) is as defined herein and the other of RX and RY (or RX2 and RY2) is a hydrogen atom (H) or C1-2alkyl. More preferably, one of RX and RY (or RX2 and RY2) is as defined herein and the other of RX and RY (or RX2 and RY2) is a hydrogen atom (H).

    [0073] Overall, for the Het group in general, it is preferred that one of RX and RY, and for Het of sub-formula (v) one of RX2 and RY2, is:

    C1-8alkyl;
    optionally substituted C3-6cycloalkyl;

    -(CH2)n3-S(O)2-R5, -CH(C1-2alkyl)-S(O)2-R5, -CMe2-S(O)2-R5, or C3-5cycloalkyl substituted at the connecting carbon atom by -S(O)2-R5; preferably -(CH2)n3-S(O)2-R5;

    -(CH2)n4-NR6R7, -CH(C1-2alkyl)-NR6R7, -CMe2-NR6R7, or C3-5cycloalkyl substituted at the connecting carbon atom by -NR6R7; preferably

    -(CH2)n4-NR6R7 or -CH(Me)-NR6R7;

    -(CH2)n11-C(O)-NR10R11, -CH(C1-2alkyl)-C(O)-NR10R11,

    -CMe2-C(O)-NR10R11, or C3-5cycloalkyl substituted at the connecting carbon atom by -C(O)-NR10R11; preferably -(CH2)n11-C(O)-NR10R11;

    -(CH2)n14-Het1, -CH(C1-2alkyl)-Het1, -CMe2-Het1, or C3-5cycloalkyl substituted at the connecting carbon atom by Het1; preferably -(CH2)n14-Het1;

    -(CH2)n10-Ar, -CH(C1-2alkyl)-Ar, -CMe2-Ar, or C3-5cycloalkyl substituted at the connecting carbon atom by Ar; preferably -(CH2)n10-Ar;

    1. (i) wherein Ar is optionally substituted phenyl, or more preferably (ii) wherein Ar is an optionally substituted 5- or 6-membered heterocyclic aromatic ring .



    [0074] Overall, for the Het group in general, it is more preferred that one of RX and RY, and for Het of sub-formula (v) one of RX2 and RY2, is:

    -(CH2)n4-NR6R7, -CH(C1-2alkyl)-NR6R7, -CMe2-NR6R7, or C3-5cycloalkyl substituted at the connecting carbon atom by -NR6R7; preferably -(CH2)n4-NR6R7 or -CH(Me)-NR6R7;

    -(CH2)n11-C(O)-NR10R11, -CH(C1-2alkyl)-C(O)-NR10R11, -CMe2-C(O)-NR10R11, or C3-5cycloalkyl substituted at the connecting carbon atom by -C(O)NR10R11; preferably -(CH2)n11-C(O)-NR10R11;

    -(CH2)n14-Het1, -CH(C1-2akyl)-Het1, -CMe2-Het1, or C3-5cycloalkyl substituted at the connecting carbon atom by Het1; preferably -(CH2)n14-Het1; or

    -(CH2)n10-Ar, -CH(C1-2alkyl)-Ar, -CMe2-Ar, or C3-5cycloalkyl substituted at the connecting carbon atom by Ar; preferably -(CH2)n10-Ar;

    1. (i) wherein Ar is optionally substituted phenyl, or or more preferably (ii) wherein Ar is an optionally substituted 5- or 6-membered heterocyclic aromatic ring.



    [0075] Optionally, one of RX and RY can be: C1-8alkyl; C3-6cycloalkyl; -(CH2)n3-SO2-R5; -(CH2)n4-NR6R7; -(CH2)n7-O-R9; -C(O)-NR10R11; -C(O)-OR13; or the 4-, 5-, 6- or 7-membered optionally substituted saturated heterocyclic ring Het1. More preferably, one of RX and RY is: C1-8alkyl; -(CH2)n3-SO2-R5; or the 4-, 5-, 6- or 7-membered optionally substituted saturated heterocyclic ring Het1. In these cases, as mentioned above, it is preferred that the other of RX and RY is a hydrogen atom (H) or C1-2alkyl.

    [0076] When RX, RX2, RY and/or RY2 is C1-8alkyl, then preferably it/they independently is/are C1-6alkyl, e.g. C3-6alkyl and/or C1-4alkyl such as methyl, isopropyl, isobutyl or t-butyl.

    [0077] When RX, RX2, RY and/or RY2 is optionally substituted C3-6cycloalkyl, then optionally it/they independently can be C3-6cycloalkyl optionally substituted by a C1-2alkyl group.

    [0078] When RX, RX2, RY and/or RY2 is optionally substituted C3-6cycloalkyl, then preferably it/they independently is/are C3-6cycloalkyl (i.e. unsubstituted), for example cyclopropyl or cyclobutyl.

    [0079] When RX, RX2, RY and/or RY2 is optionally substituted -(CH2)n2a-C3-6cycloalkyl, then preferably it/they independently is/are -(CH2)n2a-C3-6cycloakyl optionally substituted, in the -(CH2)n2a- moiety or in the C3-6cycloalkyl moiety, by a C1-2alkyl group, wherein n2a is 1, 2 or 3.

    [0080] When RX, RX2, RY and/or RY2 is optionally substituted -(CH2)n2a-C3-6cycloalkyl; then n2a is preferably 1 or 2 or more preferably 1; and/or preferably RX, RX2, RY and/or RY2 independently is/are optionally substituted -(CH2)n2a-C5-6cycloalkyl or optionally substituted -(CH2)n2a-C6cycloalkyl. When RX, RX2, RY and/or RY2 is optionally substituted -(CH2)n2a-C3-6cycloalkyl, then preferably it/they independently is/are -(CH2)n2a-C3-6cycloalkyl (i.e. not substituted). More preferably RX, RX2, RY and/or RY2 independently is/are (cyclohexyl)methyl-, that is -CH2-cyclohexyl. When RX, RX2, RY and/or RY2 is -(CH2)n3-S(O)2-R5, -CH(C1-2alkyl)-S(O)2-R5 (e.g. -CH(Me)-S(O)2-R5), -CMe2-S(O)2-R5, or C3-5cycloalkyl substituted at the connecting carbon atom by -S(O)2-R5, then preferably it/they independently is/are -(CH2)n3-S(O)2-R5.

    [0081] When RX, RX2, RY and/or RY2 is C3-5cycloalkyl substituted at the connecting carbon atom by -S(O)2-R5, then preferably it/they independently is/are C3cycloalkyl. (cyclopropyl) substituted at the connecting carbon atom by -S(O)2-R5, for example

    (see for example Example 178).

    [0082] When RX, RX2, RY and/or RY2 is -(CH2)n3-S(O)2-R5, then preferably n3 is 1.

    [0083] Preferably, R5 is C1-4alkyl (e.g. C1-3alkyl), -NR15R16, or optionally substituted phenyl. R5 is more preferably C1-3alkyl or -NH-C1-2alkyl or phenyl; still more preferably R5 is C1-3alkyl or C1-2alkyl such as methyl. Most preferably, -(CH2)n3-S(O)2-R5 is -CH2SO2Me.

    [0084] Preferably, R15 is H, C1-4alkyl (e.g. C1-2alkyl), optionally substituted phenyl or optionally substituted benzyl; and/or preferably R16 is H or methyl, e.g. H.

    [0085] When R15 and R16 together are -(CH2)n3a-X3a-(CH2)n3b-, then: preferably n3a and/or n3b independently are 2; and/or preferably X3a is a bond, -CH2-, O, or NR8a wherein R8a is C1-2alkyl or acetyl; and/or preferably the ring formed by NR15R16 is not substituted on a ring carbon or is substituted on a ring carbon by one methyl or oxo (=O) substituent.

    [0086] When RX, RX2, RY and/or RY2 is -(CH2)n4-NR6R7, -CH(C1-2alkyl)-NR6R7 (e.g. -CH(Me)-NR6R7), -CMe2-NR6R7, or C3-5cycloalkyl (e.g. C3cycloalkyl) substituted at the connecting carbon atom by -NR6R7, then preferably it/they independently is/are -(CH2)n4-NR6R7, -CH(C1-2alkyl)-NR6R7 (e.g. -CH(Me)-NR6R7), or -CMe2-NR6R7; more preferably it/they independently is/are -CH(Me)-NR6R7 or still more preferably -(CH2)n4-NR6R7.

    [0087] When RX, RX2, RY and/or RY2 is -(CH2)n4-NR6R7, then preferably n4 is 0 only when the -(CH2)n4-NR6R7 is bonded to a carbon atom in the Het ring.

    [0088] When RX, RX2, RY and/or RY2 is -(CH2)n4-NR6R7, then preferably n4 is 0, 1 or 2; more preferably n4 is 0 or 1, still more preferably n4 is 1.

    [0089] In one optional embodiment of the invention, R6 and R7 independently are H, C1-6alkyl e.g. C1-4alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, -C(O)-C1-2alkyl, -SO2-C1-2alkyl, phenyl, or benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy); or R6 and R7 together are -(CH2)n5-X5-(CH2)n6- in which n5 and n6 independently are 2 or 3 and X5 is a bond, -CH2-, O, or NR8 wherein R8 is H or C1-2alkyl, and wherein the ring formed by NR6R7 is not substituted on a ring carbon.

    [0090] In one optional embodiment of the invention, R6 and R7 independently are H, C1-6alkyl e.g. C1-4alkyl, -C(O)-C1-2alkyl or -SO2-C1-2alkyl; or R6 and R7 together are -(CH2)n5-X5-(CH2)n6- in which n5 and n6 independently are 2 or 3 and X5 is a bond, -CH2-, O, or NR8 wherein R8 is H or C1-2alkyl, and wherein the ring formed by NR6R7 is not substituted on a ring carbon.

    [0091] R6 is preferably H or C1-6alkyl. R7 is preferably C1-6alkyl, -C(O)R17 or -S(O)2R18, for example C1-6alkyl. Where R6 and/or R7 is C1-6alkyl, then it/they independently is/are preferably C1-4alkyl e.g. methyl.

    [0092] Preferably, R17 and R18 independently are C1-6alkyl (e.g. C1-4alkyl or C1-2alkyl or isopropyl or n-propyl), C3-6cycloalkyl, optionally substituted 5-membered heteroaryl being furyl (furanyl, e.g. 2-furyl) or thienyl (e.g. 2- or 3- thienyl) (the furyl or thienyl being independently optionally substituted by one oxo and/or one or two methyl), or phenyl or benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two substituents independently being fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy).

    [0093] In an alternative preferable embodiment, R6 and R7 together are -(CH2)n5-X5-(CH2)n6-, in which case it is preferable that n5 is 2 and/or n6 is 2. Preferably, when R6 and R7 together are -(CH2)n5-X5-(CH2)n6-, and when the ring formed by NR6R7 is substituted on a ring carbon by one or two substituents being oxo (=O), then the one or two oxo substituents are substituted on a ring carbon atom adjacent to (bonded to) the connecting nitrogen N of NR6R7. When R6 and R7 together are -(CH2)n5-X5-(CH2)n6-, then preferably the ring formed by NR6R7 is optionally substituted on a ring carbon by one or two substituents independently being methyl or oxo (=O) only when X5 is a bond or -CH2-.

    [0094] When R6 and R7 together are -(CH2)n5-X5-(CH2)n6-, it is preferable that the ring formed by NR6R7 is not substituted on a ring carbon or is substituted on a ring carbon by one methyl or oxo (=O) substituent.

    [0095] Preferably, R8 is C1-2alkyl or phenyl.

    [0096] For example, -(CH2)n4-NR6R7, -CH(C1-2alkyl)-NR6R7 or -CMe2-NR6R7 can be: -CH2-NHC(O)R17, -CH2-NMeC(O)R17, -CH(Me)-NHC(O)R17, -CH2-NHS(O)2R18, -CH2-NMeS(O)2R18, -CH(Me)-NHS(O)2R18, NMe2 (n4 = 0; R6 = R7 = Me), or -CH2NMe2 (n4 = 1; R6 = R7 = Me), or

    (n4 = 1; R6 and R7 together are -(CH2)2-N(Me)-(CH2)2-), or

    (n4 = 1; R6 and R7 together are -(CH2)2-O-(CH2)2-), or

    or



    [0097] When RX, RX2, RY and/or RY2 is -(CH2)n7-O-R9, then in one embodiment n7 is 1, 2 or 3 and/or R9 is H, C1-6alkyl or phenyl, or more preferably R9 is H or C1-6alkyl. n7 is preferably 1 or 2, more preferably 1. R9 is preferably C1-4alkyl such as methyl or t-butyl. For example, -(CH2)n7-O-R9 can be -CH2-O-tBu or -CH2-O-Me.

    [0098] When RX, RX2, RY and/or RY2 is -(CH2)n11-C(O)-NR10R11, -CH(C1-2alkyl)-C(O)-NR10R11 (e.g. -CH(Me)-C(O)-NR10R11), -CMe2-C(O)-NR10R11, or C3-5cycloalkyl (e.g. C3cycloalkyl) substituted at the connecting carbon atom by -C(O)-NR10R11, then: preferably it/they independently is/are -(CH2)n11-C(O)-NR10R11, -CH(C1-2alkyl)-C(O)-NR10R11 (e.g. -CH(Me)-C(O)-NR10R11), or -CMe2-C(O)-NR10R11; more preferably -(CH2)n11C(O)-NR10R11; still more preferably -CH2-C(O)-NR10R11 or -C(O)-NR10R11.

    [0099] When RX, RX2, RY and/or RY2 is -(CH2)n11-C(O)-NR10R11, then n11 is preferably 0 or 1, more preferably 1.

    [0100] Preferably R10 is H or C1-6alkyl (e.g. C1-4alkyl or C1-2alkyl or methyl), or R10 and

    [0101] R11 together are -(CH2)n8-X6-(CH2)9-.

    [0102] Preferably, R10 and R11 independently are, and more preferably R11 is:

    H; C1-6alkyl; C1-2fluoroalkyl; C2-3alkyl substituted by one OH or -OC1-2alkyl other than at the connection point; C3-6cycloalkyl optionally substituted by one or two methyl groups; -CH2-C3-6cycloalkyl optionally substituted by one NHMe group (preferably unsubstituted); -(CH2)n17-Het2; optionally substituted carbon-linked-pyridinyl, optionally substituted phenyl; optionally substituted benzyl; or optionally substituted -CH(C1-2alkyl)Ph.



    [0103] More, preferably, R10 and R11 independently are, and still more preferably R11 is: H; C1-6alkyl; C3-6cycloalkyl optionally substituted by one or two methyl groups; -CH2-C3-6cycloalkyl (unsubstituted); -(CH2)n17-Het2; optionally substituted carbon-linked-pyridinyl; optionally substituted phenyl, optionally substituted benzyl; or optionally substituted -CH(C1-2alkyl)Ph (e.g. optionally substituted -CH(Me)Ph).

    [0104] Preferably, in R10 and/or R11, the phenyl, the benzyl and the -CH(C1-2alkyl)Ph (e.g. -CH(Me)Ph) are independently optionally substituted on the aromatic ring by one or two substituents independently being: fluoro, chloro, C1-2alkyl (e.g. methyl), C1fluoroalkyl (e.g. CF3), C1-2alkoxy (e.g. methoxy), C1fluoroalkoxy (e.g. CF3O- or CHF2O-), -NR10aR10b (wherein R10a is H or methyl and R10b is H, C1-2alkyl (e.g. methyl), -C(O)Me or -S(O)2Me), -C(O)-NR10cR10d (wherein R10c and R10d independently are H or C1-2alkyl, e.g. H or Me), or -S(O)2-R10e (wherein R10e is C1-2alkyl (e.g. methyl), NH2, NHMe or NMe2). One substituent is preferred.

    [0105] In R10 and/or R11, and/or (independently) in R5, and/or (independently) in R15, and/or (independently) in R6 and/or R7, and/or (independently) in R17, and/or (independently) in R18: the carbon-linked-pyridinyl is preferably optionally substituted by one OH (including any keto tautomer thereof), and more preferably is not substituted.

    [0106] In R10 and/or R11, for -(CH2)n17-Het2, preferably n17 is 0 or 1; and/or preferably Het2 is a 5- or 6- membered saturated optionally substituted heterocyclic ring containing one O or S (preferably O) ring atom or one NR27 ring group. Preferably, R27 is C1-2alkyl or -C(O)Me. Preferably, the Het2 ring is substituted on a ring carbon by one or two substituents being methyl or is not substituted on a ring carbon.

    [0107] In one embodiment when RX, RX2, RY and/or RY2 is -(CH2)n11-C(O)-NR10R11, -CH(C1-2alkyl)-C(O)-NR10R11 or -CMe2-C(O)-NR10R11, then optionally: R10 and R11 independently are H or C1-6alkyl; or R10 and R11 together are -(CH2)n8-X6-(CH2)n9- in which n8 and n9 independently are 2 or 3 and X6 is a bond, -CH2-, O, or NR12 wherein R12 is H or C1-2alkyl, and wherein the ring formed by NR10R11 is not substituted on a ring carbon.

    [0108] Preferably R10 is H and/or optionally R11 is C1-6alkyl e.g. C1-4alkyl such as isopropyl. For example, -(CH2)n11C(O)-NR10R11 such as -C(O)-NR10R11 can be

    In an alternative preferable embodiment, when R10 and R11 together are -(CH2)n8-X6-(CH2)n9-, then preferably n8 is 2 and/or n9 is 2. When R10 and R11 together are -(CH2)n8-X6-(CH2)n9-, which is a preferable feature of the invention, then preferably X6 is a bond, -CH2-, O, or NR12 wherein R12 is H or C1-2alkyl, and wherein the ring formed by NR10R11 is not substituted on a ring carbon.

    [0109] When R10 and R11 together are -(CH2)n8-X6-(CH2)n9-, it is preferable that the ring formed by NR10R11 is not substituted on a ring carbon or is substituted on a ring carbon by one methyl or oxo (=O) substituent.

    [0110] Most preferably, NR10R11 is: NH2, NHMe, NMe2, NHEt, NHiPr,







    (In the above-illustrated most preferred groups, and generally in this specification for a group or radical, where NH or N are underlined, then this indicates the connection point.)

    [0111] Still more preferably, When RX, RX2, RY and/or RY2 is -(CH2)n11-C(O)-NR10R11, -CH(C1-2alkyl)-C(O)-NR10R11, -CMe2-C(O)-NR10R11, or C3-5cycloalkyl substituted at the connecting carbon atom by -C(O)-NR10R11, then preferably it/they independently is/are -(CH2)n11-C(O)-NR10R11 (more preferably -CH2-C(O)-NR10R11 or -C(O)-NR10R11) wherein NR10R11 is one of the above-illustrated most preferred NR10R11 groups.

    [0112] The -(CH2)n11-C(O)-NR10R11 group is preferably as defined in any of Examples 36, 58, 84, 85-90, 95-96, 126-147 or 148-155. These Examples illustrate some of the above-illustrated preferred NR10R11 groups, and some of these Examples give literature references and/or commercial sources for amines R10R11NH, which may be used to prepare the compounds of Formula (I) containing the -(CH2)n11-C(O)-NR10R11 group as RX, RX2, RY and/or RY2.

    [0113] When RX, RX2, RY and/or RY2 is -(CH2)n12-C(O)-OR13 , n12 is preferably 0 or 1, more preferably 1. In one preferred embodiment when RX, RX2, RY and/or RY2 is -(CH2)n12-C(O)-OR13 , R13 is H or C1-6alkyl. When R13 is C1-6alkyl, then R13 is preferably C1-4alkyl or C1-3alkyl such as methyl (e.g. RX, RY and/or RX2 can be -CO2Me) or ethyl.

    [0114] When RX, RX2, RY and/or RY2 is -(CH2)n13-C(O)-R13a, n13 is preferably 0 or 1, more preferably 1. When RX, RX2, RY and/or RY2 is -(CH2)n13-C(O)-R13a, then suitably R13a is C1-6alkyl, C1-2fluoroalkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, benzyl, or phenyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two of (independently) (e.g. one of) fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy). More preferably R13a is C1-6alkyl or C1-4alkyl or C1-2alkyl.

    [0115] When RX, RX2, RY and/or RY2 is -(CH2)n14-Het1, -CH(C1-2alkyl)-Het1 (e.g. -CH(Me)-Het1), -CMe2-Het1, or C3-5cycloalkyl (e.g. C3cycloalkyl) substituted at the connecting carbon atom by Het1, wherein n14 is 0, 1 or 2, then: (a) n14 is preferably 0 or 1, and/or (b) -(CH2)n14-Het1 is more preferred than -CH(Me)-Het1 or -CMe2-Het1, or C3-5cycloalkyl substituted at the connecting carbon atom by Ar.

    [0116] When RX, RX2, RY and/or RY2 is -(CH2)n14-Het1, -CH(C1-2alkyl)-Het1 (e.g. -CH(Me)-Het1), -CMe2-Het1, or C3-5cycloalkyl (e.g. C3cycloalkyl) substituted at the connecting carbon atom by Het1, wherein n14 is 0,1 or 2 and wherein Het1 is the 4-, 5-, 6- or 7-membered optionally substituted saturated heterocyclic ring containing one O or S ring atom and/or one NR14 ring group, then the optionally substituted saturated heterocyclic ring Het1 is preferably 4-, 5- or 6-membered, more preferably preferably 5-or 6-membered. When Het1 is 6-membered, then any O or S ring atom and/or any NR14 ring group independently can be present at the 2-, 3- or 4- ring position, preferably at the 4- ring position, with respect to the connecting ring-atom in Het1. When the optionally substituted saturated heterocyclic ring Het1 is 4-membered, then preferably the heterocyclic ring Het1 is not optionally substituted by oxo (=O).

    [0117] When R14 and/or a or the optional ring substituent is C1-4alkyl, it is suitably C1-2alkyl such as methyl. Preferably, R14 is C1-4akyl (e.g. C1-2alkyl), C(O)R19 or S(O)2R19. Preferably, R19 is C1-4alkyl (e.g. methyl or isobutyl), C3-6cycloalkyl such as cyclopropyl or cyclohexyl, 2-thienyl, furan-2-yl, phenyl (unsubstituted), or benzyl (unsubstituted); more preferably R19 is C1-4alkyl (e.g. methyl or isobutyl).

    [0118] When RX, RX2, RY and/or RY2 is -(CH2)n14-Het1 and n14 is 0, and when the saturated heterocyclic ring Het1 is optionally substituted (at a position other than any NR14 position) by C1-4alkyl, then preferably the optional C1-4alkyl is substituted at the carbon atom directly attached to the 5-membered ring in sub-formula (i), (ii), (iii), (iv) or (v) of Het.

    [0119] The heterocyclic ring Het1 is preferably optionally substituted (at a position or positions other than any NR14 position) by one oxo (=O) and/or one C1-4alkyl substituent; preferably by one oxo (=O) substituent. Any oxo (=O) substituent is preferably substituted on a ring carbon adjacent to (bonded to) any NR14 ring group present. Preferably, in Het1, the one or two oxo (=O) substituents are only present when there is a NR14 ring group present.

    [0120] For example, when RX, RX2, RY and/or RY2 is -(CH2)n14-Het1, -CH(C1-2alkyl)-Het1, or -CMe2-Het1, the 4-, 5-, 6- or 7-membered optionally substituted saturated heterocyclic ring Het1 can preferably be: tetrahydro-2H-pyranyl such as tetrahydro-2H-pyran-4-yl, tetrahydrofuran-2-yl, tetrahydrofuran-3-yl,







    or a positional isomer of any of the foregoing wherein the connection point [which connects to the -(CH2)n14-, -CH(C1-2alkyl)- or -CHMe2- or connecting-C3-5cycloalkyl moiety or connects to the 5-membered ring of sub-formula (i), (ii), (iii), (iv) or (v) in Het] is at a different ring carbon atom of Het1.

    [0121] When RX, RX2, RY and/or RY2 is -(CH2)n10-Ar, -CH(C1-2alkyl)-Ar (e.g. -CH(Me)-Ar), -CMe2-Ar, or C3-5cycloalkyl (e.g. C3cycloalkyl) substituted at the connecting carbon atom by Ar, then preferably it/they independently is/are -(CH2)n10-Ar or -CH(Me)-Ar, preferably -(CH2)n10-Ar such as -CH2-Ar.

    [0122] When RX, RX2, RY and/or RY2 is -(CH2)n10-Ar then preferably n10 is 0 or 1; more preferably n10 is 1.

    [0123] When Ar is optionally substituted phenyl, preferably the phenyl is optionally substituted by one or two substituents (preferably one) independently being fluoro, chloro, bromo, C1-2alkyl, C1 fluoroalkyl, C1-2alkoxy, C1fluoroalkoxy, -NR11aR11b (wherein R11a is H or methyl and R11b is H, C1-2alkyl, -C(O)Me or -S(O)2Me), -C(O)-NR11cR11d (wherein R11c and R11d independently are H or methyl), -C(O)-OR11e wherein R11e is H, or -S(O)2-R11f (wherein R11f is methyl, NH2, NHMe or NMe2). When Ar is optionally substituted phenyl, more preferably -(CH2)n10-Ar can be as defined for RX, RX2, RY and/or RY2 in any of Examples 49-55, 83, 103, 107, 120-125, 179, 181-184, 189 or 190.

    [0124] When Ar is phenyl optionally substituted at two adjacent Ar ring atoms by the two ends of a chain which is: -(CH2)4-, -(CH2)3-, or -CH=CH-CH=CH-, then it can be for example naphthyl e.g. 1-naphthyl or 2-naphthyl.

    [0125] When Ar is the optionally substituted 5- or 6-membered heterocyclic aromatic ring containing 1, 2, 3 or 4 heteroatoms (e.g. 1, 2 or 3 heteroatoms) selected from O, N or S , then Ar can be optionally substituted: furyl, thienyl, pyrrolyl, 1,3-oxazolyl, 1,3-thiazolyl, imidazolyl, oxadiazolyl (e.g. 1,3,4- or 1,2,4- or 1,2,5- oxadiazolyl), thiadiazolyl (e.g. 1,3,4- or 1,2,4-), pyridyl, triazolyl (e.g. 1,2,3- or 1,2,4- triazolyl), tetrazolyl, triazinyl, pyridazyl, pyrimidinyl, pyrazolyl, isothiazolyl (1,2-thiazolyl), or isoxazolyl (1,2-oxazolyl). When Ar is the optionally substituted 5- or 6-membered heterocyclic aromatic ring, the ring is preferably optionally substituted by one or two independent C1-2alkyl groups or by one OH group (including any keto tautomer thereof); more preferably the ring is optionally substituted by one or two independent C1-2alkyl (e.g. methyl) groups; and still more preferably there is/are one or no substituents. When Ar is the optionally substituted 5- or 6-membered heterocyclic aromatic ring, preferably it is 5-membered.

    [0126] When Ar is the 5- or 6-membered heterocyclic aromatic ring, more preferably -(CH2)n10-Ar can be as defined for RX, RX2, RY and/or RY2 in any of Examples 71, 79, 80, 97-100, 104-106, 108, 112-114, 117, 158 or 186.

    [0127] When the heterocyclic aromatic ring Ar is substituted at two adjacent Ar ring atoms by the two ends of a chain which is: -(CH2)4-, -(CH2)3-, or -CH=CH-CH=CH-, then e.g. Ar can be

    or -(CH2)n10-Ar can be

    (see for example Example 186). Preferably, in these cases -(CH2)n10-Ar is -CH2-Ar.

    [0128] In R5, R15, R6, R7, R17, R18, R9, R13, R13a, and/or R19, independent of each other, the phenyl and/or benzyl is/are preferably independently optionally substituted by one substituent; or more preferably the phenyl and/or benzyl is/are not substituted. In R10 and/or R11, independent of each other, the phenyl, benzyl and/or -CH(C1-2alkyl)Ph is/are preferably independently optionally substituted by one substituent; or more preferably the the phenyl, benzyl and/or -CH(C1-2alkyl)Ph is/are not substituted. In Ar, the phenyl and/or the heterocyclic aromatic ring is/are preferably independently optionally substituted by one substituent; or more preferably the phenyl and/or the heterocyclic aromatic ring is/are not substituted. In Het1 and/or Het2, independent of each other, the saturated heterocyclic ring is/are preferably independently optionally substituted on a ring carbon by one substituent; or more preferably the saturated heterocyclic ring is/are not substituted on a ring carbon.

    [0129] When Het is of sub-formula (v), then suitably RX2 and/or RY2 independently is/are: a hydrogen atom (H), C1-6alkyl (e.g. C1-4alkyl such as methyl), C3-6cycloalkyl, -C(O)-NR10R11, -C(O)-OR13, or -(CH2)n10-Ar; more preferably H, C1-6alkyl, -C(O)-NR10R11, -C(O)-OR13, or -(CH2)n10-Ar; still more preferably H, C1-6alkyl (e.g. C1-4alkyl such as methyl), -C(O)-NR10R11, or -(CH2)n10-Ar. In this instance, i.e. when Het is of sub-formula (v), then Ar is preferably optionally substituted phenyl and/or n10 is preferably 0 or 1.

    [0130] Preferably, RX1 and/or RY1 independently is/are a hydrogen atom (H) or C1-2alkyl, more preferably H or methyl, still more preferably H.

    [0131] Suitably, Y5 can be CH2 or CMe2. More preferably, Y5 is CH2, i.e. CRY1RY2 wherein RY1 = RY2 = a hydrogen atom (H).

    [0132] X5 can suitably be CHRX2 or CMe2, for example CHMe, CH-CO2Me or CMe2.

    [0133] It is particularly preferred that the compound of formula (I) or the salt thereof is:

    N-Cyclopentyl-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclopentyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclopentyl-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclopentyl-1-ethyl-5-(5-methyl-1,3,4-thiadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclopentyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-thiadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclopcntyl-1-ethyl-5-(5-isopropyl-1,3,4-thiadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclohexyl-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclohexyl-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-N-cyclohexyl-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-N-cyclopentyl-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclohexyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridln-4-amine,

    1-Ethyl-5-(3-methyl-1,2,4-oxadiazol-5-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[3-(methoxymethyl)-1,2,4-oxadiazol-5-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-{3-[(Dimethylamino)methyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[3-(morpholin-4-ylmethyl)-1,2,4-oxadiazol-5-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-Cyclopropyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(3-methyloxetan-3-yl)-1,3,4-oxadiazol-2-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(4-methylpiperazin-1-yl)methyl]1,3,4-oxadiazol-2-yl}-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridm-5-yl]-N-isopropyl-1,3,4-oxadiazole-2-carboxamide,

    4-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-1-methylpyrrolidin-2-one,

    1-Ethyl-N-tetrahydro-2H-pyran-4-yl-5-(5-tetrahydro-2H-pyran-4-yl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(morpholin-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-[5-(Tert-butoxymethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine, or
    methyl 2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxylate;
    or a salt thereof, e.g. a pharmaceutically acceptable salt thereof.



    [0134] Alternatively, the compound of formula (I) or the salt thereof can preferably be:

    Methyl 2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-4,5-dihydro-1,3-oxazole-4-carboxylate,

    1-Ethyl-5-(4-methyl-4,5-dihydro-1,3-oxazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-(n-Propyl)-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(tetrahydrofuran-2-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(dimethylamino)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(5-methyl-1,2,4-triazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-(3-methyl-1,2,4-oxadiazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine, or

    N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-[3-(morpholin-4-ylmethyl)-1,2,4-oxadiazol-5-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine;

    or a salt thereof, e.g. a pharmaceutically acceptable salt thereof.

    [0135] Alternatively, the compound of formula (I) or the salt thereof can preferably be:

    1-Ethyl-5-[(4R)-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(4S)-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(4S)-4-(phenylmethyl)-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(4R)-4-(phenylmethyl)-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(4S,5R)-5-methyl-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyndin-4-amine,

    1-Ethyl-5-[(5R)-5-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(5S)-5-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(4,4-Dimethyl-4,5-dihydro-1,3-oxazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxylic acid,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(1-methylethyl)-1,3-oxazole-4-carboxamide,

    1-Ethyl-5-[4-(4-morpholinylcarbonyl)-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    trans-4-{[1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-yl]amino}cyclohexanol,

    1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-3-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    4-{[1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-yl]amino}cyclohexanone,

    5-[5-(1,1-Dimethylethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-6-methyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-6-methyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-Cyclobutyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-2-pyrrolidinone,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)acetamide,

    1-Ethyl-5-[5-(1-methyl-2-piperidinyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(4-methyl-1,2,5-oxadiazol-3-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    3-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}cyclopentanone,

    1-Ethyl-5-[5-(tetrahydro-3-furanyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    (4S)-4-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo [3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-1,3-thiazolidin-2-one,

    5-[5-(2,2-Dimethylcyclopropyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-({5-[1-Etinyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)-N-methylacetamide,

    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyidin-4-amine,

    1-Ethyl-5-[5-(1-methylcyclobutyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(3-methyl-5-isoxazolyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(1-methyl-1H-pyrazol-5-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-[5-(1-Acetyl-4-piperidinyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[(4-methyl-1-piperazinyl)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[3-(4-fluorophenyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine, or

    1-Ethyl-5-{3-[2-oxo-2-(1-pyrrolidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine;

    or a salt thereof, e.g. a pharmaceutically acceptable salt thereof. For these compounds / salts, the structures of each, as a compound, are disclosed in Examples 49 to 59, 61, 62, 63, and 65 to 84 hereinafter.

    [0136] Alternatively, the compound of formula (I) or the salt thereof can preferably be:

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-phenylacetamide,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(1-phenylethyl)acetamide,

    1-Ethyl-5-{3-[2-oxo-2-(1-piperidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(phenylmethyl)acetamide,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N,N-dimethylacetamide,

    N-Ethyl-2-{5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}acetamide,

    1-Ethyl-5-{3-[1-(4-morpholinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-[3-(Cyclohexylmethyl)-1,2,4-oxadiazol-5-yl]-1-ethyl-N-(tetahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[2-oxo-2-(1-piperidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-ethyl-5-{3-[2-(4-methyl-1-piperazinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(1H-1,2,3-triazol-1-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-{5-[(2,4-Dimethyl-1,3-thiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(2-furanylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(3-isoxazolylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-ethyl-5-(5-{[4-(methyloxy)phenyl]methyl}-1,3,4-oxadiazol-2-yl)-N-(tetahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(1H-tetrazol-1-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(5-isothiazolylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(3-methyl-5-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-{[4-(Dimethylamino)phenyl]methyl}-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (1:1),

    1-Ethyl-5-{5-[(2-methyl-1,3-thiazol-4-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-[1-({5-[1-ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)cyclopentyl]-N-methylacetamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)cyclopropanecarboxamide,

    1-Ethyl-5-{5-[(5-methyl-3-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(5-methyl-3-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[2-(4-methyl-1,3-thiazol-5-yl)ethyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-{5-[(3,5-Dimethyl-4-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-(1-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}ethyl)acetamide,

    5-{5-[(1-acetyl-4-piperidinyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(4-methylphenyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(4-methylphenyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-[5-(3,4-Dimethylphenyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-[5-(2,4-Dimethylphenyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-{5-[(4-Bromophenyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(phenylmethyl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{[4-(methyloxy)phenyl]methyl}-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(2-methylphenyl)methyl]-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(4-methylphenyl)methyl]-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(3-methylphenyl)methyl]-1,3-oxazole-4-carboxamide,

    N-[(4-Chlorophenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    N-[(2,3-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    N-[(3,5-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    N-[(3,4-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(1-phenylethyl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{(1R)-1-[4-(methyloxy)phenyl]ethyl}-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(1R)-I-phenylpropyl]-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(4-methylphenyl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-({4-[(methylsulfonyl)amino]phenyl}methyl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{[4-(methylsulfonyl)phenyl]methyl}-1,3-oxazole-4-carboxamide,

    N-(1-Acetyl-4-piperidinyl)-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(tetrahydro-2-furanylmethyl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[2-(4-methyl-1-piperazinyl)ethyl]-1,3-oxazole-4-carboxamide,

    N-[1-(Aminomethyl)cyclohexyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-methyl-1,3-oxazole-4-carboxamide,

    N-(2,6-Dimethylphenyl)-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    N-{[4-(Aminocarbonyl)phenyl]methyl}-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadinol-3-yl}-N-(tetahydro-2H-pyran-4-yl)acetamide,

    5-{3-[2-(2,6-Dimethyl-4-morpholinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[2-(4-methyl-1-piperidinyl)-2-oxoediyl]-1,2,4-oxadiazol-5-yl}-N-(tetahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-[1-methyl-2-(methyloxy)ethyl]acetamide,

    5-{3-[2-(3,5-Dimethyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[2-(3-methyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-3-pyridinylacetamide,

    6-{5-[1-Ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-2-piperidinone,

    1-Ethyl-5-{5-[(3-methyl-1H-1,2,4-tiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)acetamide,

    N-({5-[1-Ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)benzamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-phenylacetamide,

    N-({5-[1-Ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-methylpropanamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-3-methylbutanamide,

    N-({5-[1-Ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)cyclohexanecarboxamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-furancarboxamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)methanesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)benzenesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-1-phenylmethanesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-propanesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamio)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-1-propanesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)cyclopropanesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-thiophenesulfonamide,

    1-({5-[1-Ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-pyrrolidinone,

    1-({5-[1-Ethyl-4-(tetrahydro-2H-pxan-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-piperidinone,

    5-{3-[(1-Acetyl-4-piperidinyl)methyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(3-{[1-(3-methylbutanoyl)-4-piperidinyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(3-{[1-(methylsulfonyl)-4-piperidinyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[1-(phenylsulfonyl)cyclopropyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[3-(phenylmethyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[3-(1-phenylethyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(3-{[4-(methyloxy)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(3-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(3-{[3-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(3-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[(phenyloxy)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[3-(5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyridin-3-ylmethyl)-1,2,4-oxadiazol-5-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[(4-phenyl-1-piperazinyl)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(5-ethyl-1,2,4-oxadiazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-3-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(5-{[4-(methyloxy)phenyl]methyl}-1,2,4-oxadiazol-3-yl)-N-(terahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine, or

    5-(3,8-Dioxa-1-azaspiro[4.5]dec-1-en-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine;

    or a salt thereof, e.g. a pharmaceutically acceptable salt thereof. The structures of each of the above-listed compounds are disclosed in Examples 85 to 191 hereinafter.

    [0137] Preferably, the compound of formula (I) or the salt thereof is:

    1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 14),

    5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 17),

    1-Ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 23),

    1-Ethyl-5-[5-(3-methyloxetan-3-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 34),

    1-Ethyl-5-{5-[(4-methylpiperazin-1-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 35),

    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 38),

    also named: 1-Ethyl-5-[5-(morpholin-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 39),

    1-Ethyl-5-[5-(tetrahydrofuran-2-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 44),

    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 77), or

    1-Ethyl-5-{3-[2-oxo-2-(1-pyrrolidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 84);

    or a salt thereof.

    [0138] A second aspect of the present invention provides a compound of formula (IA) or a salt thereof (in particular, a pharmaceutically acceptable salt thereof):

    wherein:

    R1 is C1-3alkyl, C1-2fluoroalkyl or -(CH2)2OH;

    R2 is a hydrogen atom (H), methyl or C1fluoroalkyl;

    R3 is optionally substituted C3-8cycloalkyl, or an optionally substituted heterocyclic group of sub-formula (aa), (bb) or (cc):

    in which n1 and n2 independently are 1 or 2; and Y is O, S, SO2, or NR4; where R4 is a hydrogen atom (H), C1-2alkyl, C1-2fluoroalkyl, C(O)NH2, C(O)-C1-2alkyl, or C(O)-C1fluoroalkyl;
    wherein in R3 the C3-8cycloalkyl is optionally substituted with one or two substituents being oxo (=O), OH, C1alkoxy, C1fluoroalkoxy, or C1-2alkyl; and wherein any OH, alkoxy or fluoroalkoxy substituent is not substituted at the R3 ring carbon attached (bonded) to the -NH- group of formula (IA);
    and wherein in R3 the heterocyclic group of sub-formula (aa), (bb) or (cc) is optionally substituted with one or two substituents being oxo (=O);
    and wherein Het is of sub-formula (i), (ii), (iii), (iv) or (v):

    wherein:

    W1, W2, W4 and W5 are N; and W3 is NRW;

    X1, X3 and X4 are N or CRX; X2 is O, S or NRX; and X5 is CRX1RX2;

    Y1, Y2 and Y3 are CRY or N; Y4 is O, S or NRY; and Y5 is CRY1RY2;

    Z1 and Z5 are O, S or NRZ; and Z2, Z3 and Z4 are N or CRZ;

    wherein:

    RW is a hydrogen atom (H);

    RX, RX2, RY and RY2 independently are:

    a hydrogen atom (H);

    C1-8alkyl;

    C3-6cycloalkyl optionally substituted by a C1-2alkyl group;

    -(CH2)n2a-C3-6cycloalkyl optionally substituted, in the -(CH2)n2a- moiety or in the C3-6cycloalkyl moiety, by a C1-2alkyl group, wherein n2a is 1, 2 or 3;

    -(CH2)n3-SO2-R5 wherein n3 is 1 or 2 and R5 is C1-3alkyl or -NH-C1-2alkyl or phenyl;

    -(CH2)n4-NR6R7 wherein n4 is 0, 1, 2 or 3, and R6 and R7 independently are H, C1-6alkyl e.g. C1-4alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl,
    -C(O)-C1-2alkyl, -SO2-C1-2alkyl, phenyl, or benzyl (wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy); or
    R6 and R7 together are -(CH2)n5-X5-(CH2)n6- in which n5 and n6 independently are 2 or 3 and X5 is a bond, -CH2-, O, or NR8 wherein R8 is H or C1-2alkyl;

    -(CH2)n7-O-R9; wherein n7 is 0, 1, 2 or 3 and R9 is H or C1-6alkyl; wherein n7 is 0 only when the -(CH2)n7-O-R9 is bonded to a carbon atom in the Het ring; and wherein n7 is not 0 when Het is of sub-formula (v) (i.e. n7 is not 0 for RX2 and for RY2);

    -C(O)-NR10R11 herein R10 and R11 independently are H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, phenyl, or benzyl (wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy);
    or R10 and R11 together are -(CH2)n8-X6-(CH2)n9- in which n8 and n9 independently are 2 or 3 and X6 is a bond, -CH2-, O, or NR12 wherein R12 is H or C1-2alkyl;

    -C(O)-OR13 wherein R13 is H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, phenyl, or benzyl (wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloral C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy);

    -C(O)-R13a wherein R13a is a hydrogen atom (H), C1-6alkyl, C1-2fluoroalkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, benzyl, or phenyl; wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy;

    a 4-, 5-, 6- or 7-membered saturated heterocyclic ring containing one O ring atom or one NR14 ring group wherein R14 is H or C1-4alkyl, said heterocyclic ring being optionally substituted (at a position or positions other than any NR14 position) by one oxo (=O) and/or one C1-4alkyl substituent; or

    -(CH2)n10-Ar wherein n10 is 0, 1 or 2 and

    (i) Ar is phenyl optionally substituted by one or two substituents being fluoro, chloro, C1-2alkyl, C1-2fluoroalkyl, C1-2alkoxy, C1-2fluoroalkoxy or cyano; or

    (ii) Ar is an optionally substituted 5- or 6-membered heterocyclic aromatic ring containing 1, 2 or 3 heteroatoms selected from O, N or S; and wherein when the heterocyclic aromatic ring Ar contains 2 or 3 heteroatoms, one is selected from O, N and S and the remaining heteroatom(s) are N; and wherein the heterocyclic aromatic ring Ar is optionally substituted by one or two C1-4alkyl groups;

    RX1 and RY1 independently are a hydrogen atom (H), C1-2alkyl or C1fluoroalkyl; and

    RZ is a hydrogen atom (H);

    provided that:

    when R3 is the heterocyclic group of sub-formula (bb), n1 is 1, and Y is NR4, then R4 is not C1-2alkyl or C1-2fluoroalkyl;

    when R3 is the heterocyclic group of sub-formula (aa) and Y is NR4, then R4 is not C(O)NH2, C(O)-C1-2alkyl or C(O)-C1fluoroalkyl;

    provided that: when R3 is the heterocyclic group of sub-formula (cc), then NHR3 is of sub-formula (L), (m) or (m1), wherein the -NH- connection point of the NHR3 group to the 4-position of the pyrazolopyridine of formula (IA) is underlined:

    and provided that:

    where R3 is optionally substituted C3-8cycloayl, then any OH, alkoxy or fluoroalkoxy substituent is at the 3-, 4- or 5-position of the R3 cycloalkyl ring (wherein, in this connection, the 1-position of the R3 cycloalkyl ring is deemed to be the connection point to the -NH- in formula (IA)).



    [0139] Examples 1-6, 9, 10, 13, 14, 17, 18, 19, 23, 24, and 28-48 are examples of compounds or salts of the second aspect of the invention (Formula (IA)).

    [0140] The preferred or optional features for the compound of formula (IA) or salt thereof are the same as or similar to the preferred or optional features for the compound or salt of formula (I), with all necessary changes (for example to the formula, to the R groups and/or to substituents) having been made. Generally, whenever formula (I) is mentioned herein, then in alternative embodiments the statement mentioning formula (I) applies to formula (IA), with all necessary changes having been made.

    [0141] Salts, solvates, isomers, tautomeric forms, molecular weights, etc.

    [0142] Because of their potential use in medicine, the salts of the compounds of formula (I) are preferably pharmaceutically acceptable. Suitable pharmaceutically acceptable salts can include acid or base addition salts.

    [0143] A pharmaceutically acceptable acid addition salt can be formed by reaction of a compound of formula (I) with a suitable inorganic or organic acid (such as hydrobromic, hydrochloric, sulfuric, nitric, phosphoric, succinic, maleic, formic, acetic, propionic, fumaric, citric, tartaric, lactic, benzoic, salicylic, glutamaic, aspartic, p-toluenesulfonic, benzenesulfonic, methanesulfonic, ethanesulfonic, naphthalenesulfonic such as 2-naphthalenesulfonic, or hexanoic acid), optionally in a suitable solvent such as an organic solvent, to give the salt which is usually isolated for example by crystallisation and filtration. A pharmaceutically acceptable acid addition salt of a compound of formula (I) can be for example a hydrobromide, hydrochloride, sulfate, nitrate, phosphate, succinate, maleate, formate, acetate, propionate, fumarate, citrate, tartrate, lactate, benzoate, salicylate, glutamate, aspartate, p-toluenesulfonate, benzenesulfonate, methanesulfonate, ethanesulfonate, naphthalenesulfonate (e.g. 2- naphthalenesulfonate) or hexanoate salt. In one embodiment, the pharmaceutically acceptable acid addition salt can be a hydrobromide, hydrochloride, sulfate, nitrate, phosphate, succinate, maleate, acetate, fumarate, citrate, tartrate, benzoate, p-toluenesulfonate, methanesulfonate or naphthalenesulfonate salt.

    [0144] A pharmaceutically acceptable base addition salt can be formed by reaction of a compound of formula (I) with a suitable inorganic or organic base (e.g. triethylamine, ethanolamine, triethanolamine, choline, arginine, lysine or histidine), optionally in a suitable solvent such as an organic solvent, to give the base addition salt which is usually isolated for example by crystallisation and filtration.

    [0145] Other suitable pharmaceutically acceptable salts include pharmaceutically acceptable metal salts, for example pharmaceutically acceptable alkali-metal or alkaline-earth-metal salts such as sodium, potassium, calcium or magnesium salts; in particular pharmaceutically acceptable metal salts of one or more carboxylic acid moieties that may be present in the the compound of formula (I).

    [0146] Other non-pharmaceutically acceptable salts, eg. oxalates, may be used, for example in the isolation of compounds of the invention, and are included within the scope of this invention.

    [0147] The invention includes within its scope all possible stoichiometric and non-stoichiometric forms of the salts of the compounds of formula (I).

    [0148] Also included within the scope of the invention are all solvates, hydrates and complexes of compounds and salts of the invention.

    [0149] Certain groups, substituents, compounds or salts included in the present invention may be present as isomers. The present invention includes within its scope all such isomers, including racemates, enantiomers and mixtures thereof.

    [0150] Certain of the groups, e.g. heteroaromatic ring systems, included in compounds of formula (I) or their salts may exist in one or more tautomeric forms. The present invention includes within its scope all such tautomeric forms, including mixtures. For example, when Het is of sub-formula (i), Y1 is CRY, and X1 is CRX wherein RX is OH, then the compounds of formula (I) or their salts include the keto form (K1), the enol form (E1), and mixtures thereof, as shown below, unless otherwise indicated; and when Het is of sub-formula (i) and Y1 is CRY wherein RY is OH, then the compounds of formula (I) or their salts include the keto form (K2), the enol or hydroxy-imine form (E2), and mixtures thereof, as shown below, unless otherwise indicated:



    [0151] Especially when intended for oral medicinal use, the compound of formula (I) can optionally have a molecular weight of 1000 or less, for example 800 or less, in particular 650 or less or 600 or less. Molecular weight here refers to that of the unsolvated "free base" compound, that is excluding any molecular weight contributed by any addition salts, solvent (e.g. water) molecules, etc.

    Synthetic Process Routes



    [0152] The following processes can be used to make the compounds of formula (I). The methods are sometimes illustrated for the circumstance where R2 is H or Me. However, some or all of these processes are thought to be usable with appropriate modification, e.g. of starting materials and reagents, for making compounds of Formula (I) wherein R2 is C1fluoroalkyl.

    Process A



    [0153] Compounds of formula (I) which are compounds of Formula I(ia) (that is, compounds of formula (I) wherein Het is of sub-formula (ia)) can be prepared by the cyclisation reaction of a compound of Formula II, for example in the presence of a dehydrating agent such as phosphorous oxychloride (POCl3) or Burgess reagent [(Methoxycarbonylsulphamoyl)triethylammonium hydroxide], and/or preferably in a suitable solvent (e.g. organic solvent, preferably anhydrous) such as acetonitrile (e.g. for POCl3) or THF and/or DMF (e.g. for Burgess reagent). The reaction may require heating, for example heating to from about 70 to about 150 °C or heating to from about 70 to about 120 °C or heating to from about 70 to about 90 °C:



    [0154] For the Formula II to Formula I(ia) cyclisation reaction, the conditions can for example be as described in (a) Examples 1-3 or 43 (POCl3 and acetonitrile), or (b) in Examples 32, 34-37, 35 (alternative synthesis), 38-40, 44, 66 or 97-125 (Burgess reagent, with THF and/or DMF).

    [0155] Compounds of Formula II may themselves be prepared by reacting a compound of Formula III with a suitably substituted hydrazine derivative of formula RYCONHNH2, under standard coupling conditions. For example a coupling reagent such as 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) may be used e.g. in the presence of hydroxybenzotriazole (HOBT), for example in a suitable solvent such as DMF:



    [0156] Where the required hydrazine derivative RYCONHNH2 is not readily available, compounds of Formula II may alternatively be prepared by initially reacting a compound of Formula III with a carbazate ROCONHNH2 such as t-butylcarbazate tBuOCONHNH2 under coupling conditions to form a compound of formula IV. For example a coupling reagent such as EDC may be used, e.g, in the presence of hydroxybenzotriazole, for example in a suitable solvent such as DMF:



    [0157] Subsequent Boc-deprotection of the resultant acid hydrazide derivative (compound of Formula IV) to afford a hydrazide derivative of Formula V, can be achieved using a dilute acid such as 2M hydrochloric acid in an organic solvent such as dioxane.

    [0158] The compound of Formula V can be converted to the compound of Formula II (the desired hydrazide derivative). This can be achieved by reaction of the compound of Formula V with an acid of formula RYCO2H under coupling conditions. For example a coupling agent such as EDC may be used e.g. in the presence of hydroxybenzotriazole (HOBT), for example in a suitable solvent such as DMF. Alternatively, an activated acid derivative of formula RYCO-X10 where X is a leaving group such as chloro (acid chloride) or -O-CO-R30 or -O-SO2-R30 (where R30 can e.g, be RY or alkyl or aryl such as methyl, t-butyl or p-methylphenyl) may be used to effect formation of a hydrazide of Formula II, through reaction with a hydrazide derivative of Formula V.

    [0159] Compounds of Formula III can be prepared by hydrolysis of an ester of Formula VI (for example RA can be C1-6alkyl such as Et), for example according to the method described by Yu et. al. in J. Med Chem., 2001, 44,1025-1027. This hydrolysis procedure usually involves reaction with a base such as sodium hydroxide or potassium hydroxide in a solvent such as ethanol or dioxane (e.g. NaOH in EtOH), one or both solvents preferably containing some water:



    [0160] Compounds of Formula VI can be prepared, e.g. according to the method described by Yu et. al. in J. Med Chem., 2001, 44, 1025-1027, by reaction of a compound of Formula VII with an amine of Formula R3R3aNH. The reaction is best carried out in the presence of a base such as triethylamine or diisopropylethyl amine in a solvent such as ethanol or dioxane (e.g. NEt3 in EtOH) and may require heating:



    [0161] Many amines of Formula R3R3aNH, e.g. those amines wherein R3R3aN are of sub-formulae (a) to (t2), are either commercially available, or syntheses therefor have been published and/or described herein, or they can be prepared from commercially available or synthesizable compounds e.g. from other amines of Formula R3R3aNH or derivatives thereof. For amines R3R3aNH whose preparations and/or specific commercial sources are described herein, see e.g. Intermediates 21, 21A, 25, 50, 54-57, and 140-163.

    [0162] Compounds of Formula VII are also described in the above reference and can be prepared first by reaction of a compound of Formula VIII with, for example, diethyl (ethoxymethylene)malonate (R2 = H, to afford RA = Et) or diethyl 2-(1-ethoxyethylidene)malonate (R2 = Me, to afford RA = Et), e.g. with heating, followed by reaction with phosphorous oxychloride, again preferably with heating. See for example Intermediate 1 synthesis and G. Yu et. al., J. Med Chem., 2001, 44, 1025-1027 hereinafter, where R2 = H and R1 = ethyl; and see Intermediate 58 synthesis hereinafter where R2 = Me and R1 = ethyl:



    [0163] Where, for example, the desired amino pyrazole of Formula VIII is not commercially available, preparation of the Formula VIII pyrazole can be achieved, for example using methods described by Dorgan et. al. in J. Chem. Soc., Perkin Trans. 1980, 1 (4), 938-42, involving reaction of cyanoethyl hydrazine with a suitable aldehyde R1aCHO in a solvent such as ethanol, with heating, followed by reduction, for example reduction with sodium in a solvent such as t-butanol. R1a should be chosen so as to contain one less carbon atom than R1, for example R1a = methyl will afford R1 = ethyl.



    [0164] Alternatively, e.g. where the desired amino pyrazole of Formula VIII is not commercially available, preparation of the compound of Formula VI can be achieved from the compound of Formula VII (e.g. Intermediate 1 wherein R1 = ethyl), using a generalised version of the reaction scheme shown in Example 43, especially that part relating to conversion of Intermediate 1 to Intermediate 38. In this method: the 4-chloro pyrazolopyridine of Formula VII (e.g. Intermediate 1) is optionally converted to the 4-alkoxy (e.g. C1-4alkoxy such as ethoxy) pyrazolopyridine (e.g. Intermediate 35); the R1 group is removed (to e.g. Intermediate 36 wherein R1 is H rather than alkyl), the 4-amino R3R3aN group is inserted by displacing the 4-chloro or 4-alkoxy group by reaction with R3R3aNH (e.g. to Intermediate 37); and the pyrazolopyridine is alkylated at N-1 by reacting it with R1-X40 where X40 is a group displaceable by the N-1 nitrogen of the pyrazolopyridine in order to re-insert the desired R1 group (e.g. Intermediate 38 synthesis). X40 can for example be a halogen, e.g. Cl, Br or I; or X40 can be -O-SO2-R40 where R40 is C1-4alkyl, C1-2fluoroalkyl, or phenyl optionally substituted by C1-2alkyl.

    Process B



    [0165] Compounds of formula (I) which are compounds of Formula I(ia) (that is, compounds of formula (I) wherein Het is of sub-formula (ia)) can alternatively be prepared by reaction of a compound of Formula IX with an amine of formula R3R3aNH, preferably in a solvent (e.g. organic solvent) such as ethanol or acetonitrile, and/or preferably in the presence of a base such as DIPEA. Heating may be required to effect the conversion:

    For the reaction of a compound of Formula IX with an amine of formula R3R3aNH to prepare the compound of Formula I(ia), the reaction conditions, e.g. solvents, mole ratios, temperatures and/or reaction times, can optionally be as described in Examples 9, 10-11 and/or 12-27.

    [0166] The reaction of Formula IX with R3R3aNH to give Formula I(ia) can be generalised for any compound of Formula (I), containing any Het group as defined herein, starting from a compound of Formula IXa:



    [0167] Compounds of Formula IX can themselves be prepared by cyclisation of a compound of Formula X, preferably in the presence of a dehydrating agent such asphosphorous oxychloride or Burgess reagent [(Methoxycarbonylsulphamoyl)triethylammonium hydroxide], in a suitable solvent (e.g. organic solvent, preferably anhydrous) such as acetonitrile (e.g. for POCl3) or THF and/or DMF (e.g. for Burgess reagent). The reaction may require heating, for example heating to from about 70 to about 150 °C or heating to from about 70 to about 120 °C or heating to from about 70 to about 90 °C:



    [0168] Compounds of Formula X can be prepared by initial activation of an acid of Formula XI, for example with an amide coupling reagent such as EDC/HOBT or with thionyl chloride, followed by reaction of the thus formed activated intermediate with an acid hydrazide of Formula RYCONHNH2:



    [0169] Examples of reactions of the compound of Formula XI to Formula X and of the compound of Formula X to Formula IX are presented in Intermediates 12 to 15.

    [0170] Acids of Formula XI can themselves be prepared by hydrolysis of an ester of Formula VII (e.g. as described in Process A) using a base such as potassium hydroxide in a solvent such as aqueous dioxane dioxane/water):


    Process C



    [0171] Compounds of Formula XII (that is, compounds of formula (I) wherein Het is of sub-formula (ib)) can be prepared by reaction of a compound of Formula II with a reagent capable of inserting sulfur, such as Lawesson's reagent, usually in a suitable solvent such as acetonitrile. The reaction may require heating:

    The reaction conditions, e.g. solvents, mole ratios, temperatures and/or reaction times, can optionally be as described in Examples 4, 5 or 6.

    Process D



    [0172] Compounds of Formula XIII [which are compounds of formula (I) wherein Het is of sub-formula (ic)] can be prepared by reaction of a compound of Formula VI (RA can be C1-6alkyl such as Et) with an amidoxime of formula RXC(=NOH)NH2 preferably in the presence of a base such as sodium ethoxide and/or preferably in a suitable solvent (e.g. anhydrous and/or organic solvent) such as ethanol, and preferably in the presence of molecular sieves (e.g. 4 Angstrom and/or powdered molecular sieves) or under other conditions effective for removing water. The reaction mixture may optionally be heated, for example to reflux:

    The reaction conditions, e.g. solvents, mole ratios, temperatures and/or reaction times, can optionally be as described in Reference Example 7 and Examples 28-29, 30, 31, 48, 82-84, 92, 93 and/or 178-187.

    Process E



    [0173] Compounds of Formula XIV (which are compounds of formula (I) wherein Het is of sub-formula (if)) can be prepared by reaction of a compound of Formula XV with a suitable acetimidate RX-C(=NH)ORE, where RE is C1-6alkyl e.g. methyl, (such as methyl acetimidate (RX = Me)), preferably in the presence of a base (such as triethylamine or sodium ethoxide) and/or in a suitable solvent (e.g. anhydrous and/or organic solvent) such as ethanol:

    Compounds of Formula XV may themselves be prepared by reaction of a compound of Formula III with a suitably substituted hydrazine derivative of Formula RZNHNH2, under coupling conditions. For example a coupling agent such as EDC may be used, e.g. in the presence of hydroxybenzotriazole (HOBT), in a suitable solvent such as DMF:


    Process F



    [0174] To make a compound of formula (I) wherein Het is of sub-formula (id) (optionally substituted 1,3-oxazol-2-yl), methods known to the skilled person can be used.

    [0175] For example, the 5-carboxylic acid compound of Formula III can be converted directly or indirectly to a compound of formula (I) wherein Het is of sub-formula (id) (i.e. to a 5-(optionally-substituted 1,3-oxazol-2-yl)-pyrazolopyridine). Alternatively or additionally, a compound of formula (I), wherein Het is of sub-formula (va) in which RX1 and RY1 are H and RX1 is RX and RY1 is RY [i.e. the corresponding 5-(optionally-substituted 4,5-dihydro-1,3-oxazol-2-yl)-pyrazolopyridine], can be dehydrogenated to a compound of formula (I) wherein Het is of sub-formula (id); e.g. by the method shown in Example 41 (DBU, CCl4, CH3CN, Pyridine) or a modification of this method or by an analogous method for example using an oxidising agent.

    [0176] The dehydrogenation (oxidation) of the 4,5-dihydro-1,3-oxazol-2-yl compound of formula (I) (wherein Het is of sub-formula (va) in which RX1 and RY1 are H and RX1 is RX and RY1 is RY) to the corresponding 1,3-oxazol-2-yl compound of formula (I)
    wherein Het is of sub-formula (id) can be carried out using reagents and conditions known to the skilled man (see for example the following reviews: T.G. Gant et al., Tetrahedron, 1994, 50(8), 2297-2360; M.Reuman et al., Tetrahedron, 1985, 41(5), 837-860; and references cited therein). For this dehydrogenation reaction, preferably an oxidising agent is used such as nickel peroxide, manganese dioxide (MnO2), or 2,3-dichloro-5,6-dicyano-1,4-benzoquinone (DDQ).

    [0177] A compound of formula (I) wherein Het is of sub-formula (va) can be prepared by cyclisation of a compound of Formula XXVIII, for example in the presence of Burgess reagent and/or preferably in a suitable solvent (e.g. organic solvent, preferably anhydrous) such as THF.



    [0178] The compound of Formula XXVIII can be prepared from the compound of Formula III by reaction with the compound of Formula XXIX under coupling conditions (e.g. EDC with or without HOBT), optionally in the presence of a base such as Et3N, and preferably in a suitable solvent such as DMF.

    Process G



    [0179] Compounds of the invention of Formula XVI (1,2,4-oxadiazoles), which are compounds of formula (I) wherein Het is of sub-formula (ic) and RX is -CH2C(O)NR10R11, can be prepared by reaction of a compound of the Formula XVII with an amine of Formula R10R11NH, under coupling conditions. Standard coupling conditions can be used known to the skilled person. For example a coupling agent such as TBTU may be used, preferably in the presence of hydroxybenzotriazole. However, it is more preferable that the coupling agent is oxalyl chloride, which in the reaction forms the corresponding acid chloride from the carboxylic acid of the compound of Formula XVII; in this embodiment it is preferable that the acid chloride is not isolated, i.e. the solvent in which it is formed is preferably not removed to a substantial extent. Preferably, whatever the coupling agent / coupling conditions, the reaction is carried out in the presence of a base such as diisopropylethylamine, and/or in a suitable solvent (e.g. organic solvent, preferably anhydrous) such as DMF and/or dicloromethane.

    The reaction conditions for the Formula XVII to Formula XVI reaction, e.g. solvents, mole ratios, temperatures and/or reaction times, can optionally be as described in Examples 85-90,95-96 and/or 148-155.

    [0180] Compounds of Formula XVII may themselves be prepared by reaction of a compound of Formula XVIII (RG is preferably tBu) with a hydrolysing agent (e.g. an acid such as trifluoroacetic acid) in a solvent such as dichloromethane:



    [0181] Compounds of Formula XVIII can be prepared by reaction of a compound of Formula VI (RA = H) with an amidoxime of formula RGOC(=O)CH2C(=NOH)NH2 and a coupling agent, for example TBTU, preferably in the presence of hydroxybenzotriazole, preferably in the presence of a base such as diisopropylethylamine and/or in a suitable solvent such as DMF, followed by reaction with 1,1'-carbonyldiimidazole:


    Process H



    [0182] Compounds of Formula XIX, which are compounds of formula (I) wherein Het is of sub-formula (ic) and RX is -CH2-NR6R7 wherein R7 is C(O)R17, may be prepared from compounds of Formula XX. For example, this can be by reaction of the compound of Formula XX with a carboxylic acid R17COOH in the presence of a coupling agent, for example TBTU, preferably with hydroxybenzotriazole, and preferably in the presence of a base such as diisopropylethylamine in a suitable solvent such as DMF. Alternatively or additionally, the compound of Formula XX can be reacted with an activated derivative of the carboxylic acid moiety of R17COOH (e.g. by reaction with an acid chloride R17C(O)Cl), preferably in the presence of a base such as diisopropylethylamine and/or in a suitable solvent (e.g. organic) such as dichloromethane and/or chloroform.

    The reaction conditions for the Formula XX to Formula XIX reaction, e.g. solvents, mole ratios, temperatures and/or reaction times, can optionally be as described in any of Examples 159-165.

    [0183] Compounds of Formula XX, which are compounds of formula (I) wherein Het is of sub-formula (ic) and RX is -CH2-NR6R7 wherein R7 is H, may be prepared by deprotecting compounds of Formula XXI wherein RH is benzyl or C1-6alkyl such as tBu, e.g. by reaction with an acid such as trifluoroacetic acid (e.g. where RH is C1-6alkyl such as tBu) or by hydrogenation (e.g. where RH is benzyl), preferably in a suitable solvent such as dichloromethane:

    Compounds of Formula XXI can be prepared by reaction of a compound of Formula VI (but wherein RA is OH) with an amidoxime of formula RHOC(=O)N(R6)CH2C(=NOH)NH2 and a coupling agent, for example TBTU, preferably in the presence of hydroxybenzotriazole, and preferably in the presence of a base such as diisopropylethylamine, and/or preferably in a suitable solvent such as DMF, followed by reaction with a base such as 1,8-diazabicyclo[5.4.0]undec-7-ene:


    Process I



    [0184] Compounds of Formula XXII, which are compounds of formula (I) wherein Het is of sub-formula (ic) and RX is -CH2-NR6R7 wherein R7 is -S(O)2R18, may be prepared from compounds of Formula XX by reaction with a sulphonyl chloride R18S(O)2Cl, preferably in the presence of a base such as triethylamine and/or pyridine, and/or preferably in a suitable solvent (e.g. organic) such as dichloromethane and/or chloroform:



    [0185] The reaction conditions, e.g. solvents, mole ratios, temperatures and/or reaction times, can optionally be as described in any of Examples 166-172.

    Process J



    [0186] Compounds of Formula XXIII are compounds of formula (I) wherein Het is of sub-formula (ic) and RX is -CH2-NR6R7, wherein R6 and R7 together are -(CH2)n5-X5-(CH2)n6- in which n5 and n6 independently are 2 or 3, and wherein the ring formed by NR6R7 is substituted by one oxo (=O) substituent at a carbon atom within (CH2)n6 which carbon atom is bonded to the nitrogen.

    [0187] Compounds of Formula XXIII can be prepared by reaction of a compound of the type Formula XX wherein R6 = H with acid chlorides of the type XJ-(CH2)n5-X5-(CH2)(n6-1)-COCl, where XJ is a leaving group, preferably in the presence of a base such as triethylamine and/or preferably in a suitable solvent, for example dichloromethane or tetrahydrofuran, preferably followed by treatment with a base such as sodium hydride in a suitable solvent such as DMF. The leaving group XJ can for example be a halogen atom such as Cl, Br or I; or XJ can for example be -O-SO2-RJ where RJ is C1-4alkyl, C1-2fluoroalkyl, or phenyl optionally substituted by C1-2alkyl e.g. 4-methylphenyl.



    [0188] For examples of reaction conditions for the Formula XX to Formula XXIII reaction, see for example Intermediates 119 and/or 120 and/or subsequent Examples 173 and/or 174.

    Process K



    [0189] Compounds of the type Formula XXIV, which are compounds of formula (I) wherein Het is of sub-formula (iia), can be prepared from compounds of the type Formula XXV by reaction with RYC(O)XK where XK is a leaving group, preferably in a solvent such as acetic acid, pyridine, diglyme and/or dichloromethane. XK can for example be chloro; or RYC(O)XK can be an anhydride such as [RY(C=O)]2O; or RYC(O)XK can be an activated carboxylic acid derivative prepared from the reaction of RYC(O)OH with a coupling reagent such as EDC or TBTU with or without the presence of HOBT.

    For the Formula XXV to Formula XXIV reaction, the reaction conditions can for example be as described in Examples 188, 189 and/or 190.

    [0190] Compounds of the type Formula XXV can be prepared from compounds of the type Formula XXVI by reaction with hydroxylamine or a hydroxylamine salt, preferably in the presence of a base such as potassium carbonate, sodium alkoxide or a tertiary amine, and/or preferably in a suitable solvent such as ethanol or methanol:

    Compounds of the type Formula XXVI may themselves be prepared from compounds of Formula XXVII by reaction with a dehydrating agent such as Burgess Reagent, preferably in a solvent, for example tetrahydrofuran:



    [0191] Compounds of the type Formula XXVII can be prepared from carboxylic acid compounds of Formula III, for example by reaction with thionyl chloride followed by ammonia in a suitable solvent such as dioxane:


    Process L - Conversion of a compound of formula (I) or a salt thereof into a different compound of formula (I) or a salt thereof



    [0192] One compound of formula (I) or salt thereof can be converted into another compound of formula (I) or salt thereof. This conversion preferably comprises or is one or more of the following processes L1 to L10:

    L1. An oxidation process. For example, the oxidation process can comprise or be oxidation of an alcohol to a ketone (e.g. using Jones reagent) or oxidation of an alcohol or a ketone to a carboxylic acid.

    L2. A reduction process, for example reduction of a ketone or a carboxylic acid to an alcohol.

    L3. Acylation, for example acylation of an amine or of a hydroxy group.

    L4. Alkylation, for example alkylation of an amine or of a hydroxy group.

    L5. Hydrolysis, e.g. hydrolysis of an ester to the corresponding carboxylic acid or salt thereof, for example in the presence of base (e.g. alkali-metal hydroxide, preferably also in the presence of water) or in the presence of acid (e.g. aqueous HCl, or HCl in an anhydrous organic solvent such as dioxane).
    The hydrolysis can for example be hydrolysis of an ester compound, in which RX, RX2, RY or RY2 is -(CH2)n12-C(O)-OR13 wherein R13 is not a hydrogen atom (H), to the corresponding carboxylic acid wherein R13 is a hydrogen atom (H). See for example Example 57 and Intermediate 83.
    The hydrolysis can for example be hydrolysis of an ester compound, wherein R3 is substituted by -C(O)OR23 in which R23 is C1-2alkyl (e.g. wherein NHR3 or NR3R3a is of sub-formula (p8) which is

    to the corresponding carboxylic acid or salt thereof wherein R23 is H (e.g. wherein NHR3 or NR3R3a is of sub-formula (p7)). L6. Deprotection, e.g. deprotection (e.g. deacylation or t-butyloxycarbonyl (BOC) removal or benzyloxycarbonyl removal) of an amine group.

    L7. Formation of an ester or amide, for example from the corresponding carboxylic acid and/or an activated derivative of the carboxylic acid (e.g. acid chloride or acid anhydride or carboxylic acid activated by a coupling agent).
    The amide formation can be formation of an amide compound, in which one or more of RX, RX2, RY and RY2 is -(CH2)n11-C(O)-NR10R11, -CH(C1-2alkyl)-C(O)-NR10R11, -CMe2-C(O)-NR10R11 or cycloalkyl substituted by -C(O)-NR10R11, from the corresponding carboxylic acid and/or an activated derivative of the carboxylic acid. For examples of this amide formation, see Examples 58-59 and/or 126-147 for Het = sub-formula (id), and/or Process G herein for Het = sub-formula (ic) (e.g. Examples 85-90, 95-96 and/or 148-155).
    The amide formation can alternatively be formation of an amide compound, in which one or more of RX, RX2, RY and RY2 is -(CH2)n4-NR6R7, -CH(C1-2alkyl)-NR6R7, -CMe2-NR6R7 or cycloalkyl substituted by -NR6R7, wherein R6 is C(O)R17, from the corresponding carboxylic acid and/or an activated derivative of the carboxylic acid. For one example where Het is of sub-formula (ic) see Process H and/or Examples 159-165.

    L8. Conversion of a ketone into the corresponding oxime or oxime ether. This can for example include conversion of an oxo (=O) substituent within R3, e.g. within the NHR3 or NR3R3a sub-formula (o), into an hydroxyimino (=N-OH) or (C1-2alkoxy)imino (=N-OR26) substituent within R3, e.g. within the NHR3 or NR3R3a sub-formula (o2), (o3) or (o4). This conversion can be carried out in the case of an oxime (hydroxyimino, =N-OH) by reacting hydroxylamine or a salt thereof (e.g. hydroxylamine hydrochloride) with the ketone, or in the case of an oxime ether (C1-2alkoxy)imino, =N-OR26) by reacting C1-2alkoxylamine or a salt thereof (e.g. hydrochloride salt) with the ketone. The reaction is preferably carried out in the presence of a base such as anhydrous potassium carbonate or diisopropylethylamine and/or in a suitable solvent such as acetonitrile. The mixture can be heated e.g. to reflux.

    L9. Sulfonylation, e.g. sulfonamide formation by reaction of an amine with a sulfonyl halide e.g. a sulfonyl chloride (e.g. see also Process I).
    and/or

    L10. Beckmann rearrangement of one compound of formula (I) into another compound of formula (I). Preferably, this uses cyanuric chloride (2,4,6-trichloro-1,3,5-triazine) together with a formamide such as DMF, e.g. at room temperature (see L.D. Luca, J. Org. Chem., 2002, 67, 6272-6274). The Beckmann rearrangement can for example comprise conversion of an (hydroxyimino)cycloalkyl compound of formula (I), e.g. wherein NHR3 or NR3R3a is of sub-formula (o2)

    into a single-atom-ring-expanded lactam compound of formula (I), e.g. wherein NHR3 or NR3R3a is of sub-formula (m3)



    [0193] The present invention therefore also provides a method of preparing a compound of formula (I) or a salt thereof, comprising:

    (a) cyclisation of a compound of formula II to a compound of formula (I) wherein Het is of sub-formula (ia) (that is: to a compound of Formula I(ia), i.e. to an optionally substituted 1,3,4-oxadiazol-2-yl derivative at the 5-position of the pyrazolopyridine ring system), for example in the presence of a dehydrating agent such as phosphorus oxychloride or Burgess reagent, or

    (b) reaction of a compound of formula IXa with an amine of formula R3R3aNH to form a compound of formula (I), preferably in a solvent (e.g. organic solvent) and/or preferably in the presence of a base, or

    (c) cyclisation of a compound of formula II to a compound of formula (I) wherein Het is of sub-formula (ib) (i.e. to a compound of Formula XII i.e. to an optionally substituted 1,3,4-thiadiazol-2-yl derivative at the 5-position of the pyrazolopyridine ring system), for example in the presence of an agent capable of introducing sulfur such as Lawesson's reagent, or

    (d) reaction of a compound of formula VI, wherein RA is C1-6alkyl such as Et, with an amidoxime of formula RXC(=NOH)NH2 or a salt thereof, preferably in the presence of a base such as sodium ethoxide and/or preferably in a suitable solvent (e.g. anhydrous and/or organic solvent) such as ethanol; or

    (e) reaction of a compound of formula XV with an acetimidate RX-C(=NH)ORE, where RE is C1-6alkyl, to prepare a compound of formula (I) wherein Het is of sub-formula (if) (i.e. to a compound of Formula XIV, i.e, to an optionally substituted 1,2,4-triazol- 3-yl or 5-yl derivative at the 5-position of the pyrazolopyridine ring system), preferably in the presence of a base (such as triethylamine or sodium ethoxide) and/or in a suitable solvent (e.g. anhydrous and/or organic solvent) such as ethanol; or

    (f)(i) converting directly or indirectly a compound of Formula III to a compound of formula (I) wherein Het is of sub-formula (id); and/or (f)(ii) dehydrogenating a compound of formula (I), wherein Het is of sub-formula (va) in which RX1 and RY1 are H and RX1 is RX and RY1 is RY, to a compound of formula (I) wherein Het is of sub-formula (id); or

    (f)(iii) cyclisation of a compound of Formula XXVIII, for example in the presence of Burgess reagent and/or preferably in a suitable solvent, to prepare a compound of formula (I) wherein Het is of sub-formula (va); or

    (g) reaction of a compound of the Formula XVII with an amine of Formula R10R11NH under coupling conditions, to prepare a compound of formula (I) wherein Het is of sub-formula (ic) and RX is -CH2C(O)NR10R11 (i.e. to prepare a compound of Formula XVI), the reaction preferably being carried out in the presence of a base such as diisopropylethylamine, and/or preferably in a suitable solvent (e.g. organic solvent, preferably anhydrous) such as DMF and/or dicloromethane, and/or preferably in the presence of oxalyl chloride; or

    (h) conversion of a compound of Formula XX into a compound of formula (I) wherein Het is of sub-formula (ic) and RX is -CH2-NR6R7 wherein R7 is C(O)R17 (i.e. into a compound of Formula XIX), preferably either by reaction of the compound of Formula XX with a carboxylic acid R17COOH in the presence of a coupling agent, and/or by reaction of the compound of Formula XX with an activated derivative of the carboxylic acid moiety of R17COOH (e.g. R17C(O)Cl), preferably in the presence of a base and/or a suitable solvent; or

    (i) reaction of a compound of Formula XX with a sulphonyl chloride R18S(O)2Cl to prepare a compound of formula (I) wherein Het is of sub-formula (ic) and RX is -CH2-NR6R7 wherein R7 is -S(O)2R18 (i.e. to prepare a compound of Formula XXII), preferably in the presence of a base such as triethylamine and/or pyridine, and/or preferably in a suitable solvent such as dichloromethane and/or chloroform; or

    (j) reaction of a compound of Formula XX wherein R6 = H with an acid chloride of formula XJ-(CH2)n5-X5-(CH2)(n6-1)-COCl, where XJ is a leaving group (XJ preferably being a halogen atom or -O-SO2-RJ where RJ is C1-4alkyl, C1-2fluoroalkyl, or phenyl optionally substituted by C1-2alkyl), to prepare a compound of formula (I) wherein Het is of sub-formula (ic) and RX is -CH2-NR6R7, wherein R6 and R7 together are -(CH2)n5-X5-(CH2)n6- in which n5 and n6 independently are 2 or 3, and wherein the ring formed by NR6R7 is substituted by one oxo (=O) substituent at a carbon atom within (CH2)n6 which carbon atom is bonded to the nitrogen (i.e. to prepare a compound of Formula XXIII); the reaction preferably being in the presence of a base and/or in a suitable solvent, and/or preferably being followed by treatment with a base; or

    (k) reaction of a compound of Formula XXV with RYC(O)XK where XK is a leaving group, to prepare a compound of formula (I) wherein Het is of sub-formula (iia) (i.e. to prepare a compound of Formula XXIV); or

    (L) conversion of a compound of formula (I) or a salt thereof into a different compound of formula (I) or a salt thereof;

    and optionally converting the compound of formula (I) into a salt e.g. a pharmaceutically acceptable salt.

    [0194] Salt formation processes may optionally be as described elsewhere herein.

    [0195] Preferred features of methods (a), (b), (c), (d), (e), (f)(i), (f)(ii), (f)(iii), (g), (h), (i), (j), (k), and (L), independently of each other, are preferably as described above for Processes A, B, C, D, E, F, G, H, I, J, K, and L with all necessary changes being made. For example, the conversion process (L) preferably comprises or is one or more of processes L1 to L10 described herein, e.g. hereinabove.

    [0196] In any of the methods which involve reaction of a carboxylic acid and/or an activated carboxylic acid derivative with an amine to form an amide, the activated carboxylic acid derivative preferably comprises a -C(O)X11 group in place of the COOH, wherein X11 is a leaving group substitutable by an amine. For example X11 can be Cl (wherein the activated derivative = the acid chloride) or -OC(O)R (wherein the activated derivative = an anhydride). Alternatively, the activated carboxylic acid derivative can be an activated ester wherein the leaving group X11 is



    [0197] The latter activated carboxylic acid derivative can be formed from the carboxylic acid (X11 = OH) either:

    (a) by reaction of the carboxylic acid with a carbodiimide such as EDC, which is 1-ethyl-3-(3'-dimethylaminopropyl)carbodiimide and is also 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, or a salt thereof e.g. hydrochloride salt, preferably followed by reaction of the resulting product with 1-hydroxybenzotriazole (HOBT); reaction (a) usually being carried out in the presence of a solvent (preferably anhydrous) such as dimethyl formamide (DMF) or acetonitrile and/or preferably under anhydrous conditions and/or usually at room temperature (e.g. about 20 to about 25 °C); or

    (b) by reaction with 2-(1H-benzotriazole-1-yl)-1,1,3,3-tetramethyluronium tetrafluoroborate (TBTU) or O-(7-Azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate (HATU), preferably in the presence of a base such as diisopropylethylamine (iPr2NEt = DIPEA), and usually in the presence of a solvent such as dimethyl formamide (DMF) or acetonitrile and/or preferably under anhydrous conditions and/or usually at room temperature (e.g. about 20 to about 25 °C).



    [0198] The present invention also provides: (m) a method of preparing a pharmaceutically acceptable salt of a compound of formula (I) comprising conversion of the compound of formula (I) or a salt thereof into the desired pharmaceutically acceptable salt thereof.

    [0199] The present invention also provides a compound of formula (I) or a salt thereof, prepared by a method as defined herein.

    Medical uses



    [0200] The present invention also provides a compound of formula (I) or a pharmaceutically acceptable salt thereof for use as an active therapeutic substance in a mammal such as a human. The compound or salt can be for use in the treatment and/or prophylaxis of any of the diseases / conditions described herein (e.g. for use in the treatment and/or prophylaxis of an inflammatory and/or allergic disease in a mammal) and/or for use as a phosphodiesterase inhibitor e.g. for use as a phosphodiesterase 4 (PDE4) inhibitor. "Therapy" may include treatment and/or prophylaxis.

    [0201] Also provided is the use of a compound of formula (I) or a pharmaceutically acceptable salt thereof in the manufacture of a medicament (e.g. pharmaceutical composition) for the treatment and/or prophylaxis of any of the diseases / conditions described herein in a mammal such as a human, e.g. for the treatment and/or prophylaxis of an inflammatory and/or allergic disease in a mammal such as a human.

    [0202] A compound of formula (I) or a pharmaceutically acceptable salt thereof can optionally be used in a method of treatment and/or prophylaxis of any of the diseases / conditions described herein in a mammal (e.g. human) in need thereof, e.g. a method of treatment and/or prophylaxis of an inflammatory and/or allergic disease in a mammal (e.g. human) in need thereof, which method comprises administering to the mammal (e.g. human) a therapeutically effective amount of the compound of formula (I) as herein defined or the pharmaceutically acceptable salt thereof.

    [0203] Phosphodiesterase 4 inhibitors are thought to be useful in the treatment and/or prophylaxis of a variety of diseases / conditions, especially inflammatory and/or allergic diseases, in mammals such as humans, for example: asthma, chronic obstructive pulmonary disease (COPD) (e.g. chronic bronchitis and/or emphysema), atopic dermatitis, urticaria, allergic rhinitis, allergic conjunctivitis, vernal conjunctivitis, eosinophilic granuloma, psoriasis, rheumatoid arthritis, septic shock, ulcerative colitis, Crohn's disease, reperfusion injury of the myocardium and brain, chronic glomerulonephritis, endotoxic shock, adult respiratory distress syndrome, multiple sclerosis, cognitive impairment (e.g. in a neurological disorder such as Alzheimer's disease), depression, or pain. Ulcerative colitis and/or Crohn's disease are collectively often referred to as inflammatory bowel disease.

    [0204] In the treatment and/or prophylaxis, the inflammatory and/or allergic disease is preferably chronic obstructive pulmonary disease (COPD), asthma, rheumatoid arthritis or allergic rhinitis in a mammal (e.g. human). More preferably, the treatment and/or prophylaxis is of COPD or asthma in a mammal (e.g. human).

    [0205] PDE4 inhibitors are thought to be effective in the treatment of asthma (e.g. see M.A.Giembycz, Drugs, Feb. 2000, 59(2), 193-212; Z. Huang et al., Current Opinion in Chemical Biology, 2001, 5: 432-438; H.J.Dyke et al., Expert Opinion on Investigational Drugs, January 2002, 11(1), 1-13; C.Burnouf et al., Current Pharmaceutical Design, 2002, 8(14), 1255-1296; A.M.Doherty, Current Opinion Chem. Biol, 1999, 3(4), 466-473; and refs cited therein).

    [0206] PDE4 inhibitors are thought to be effective in the treatment of COPD (e.g. see S.L. Wolda, Emerging Drugs, 2000, 5(3), 309-319; Z. Huang et al., Current Opinion in Chemical Biology, 2001, 5: 432-438; H.J.Dyke et al., Expert Opinion on Investigational Drugs, January 2002, 11 (1), 1-13; C.Burnouf et al., Current Pharmaceutical Design, 2002, S(14), 1255-1296; A.M.Doherty, Current Opinion Chem. Biol., 1999, 3(4), 466-473; and refs cited therein). COPD is often characterised by the presence of airflow obstruction due to chronic bronchitis and/or emphysema (SL Wolda, Emerging Drugs, 2000, 5(3), 309-319).

    [0207] PDE4 inhibitors are thought to be effective in the treatment of allergic rhinitis (e.g. see B.M. Schmidt et al., J. Allergy & Clinical Immunology, 108(4), 2001, 530-536).

    [0208] PDE4 inhibitors are thought to be effective in the treatment of rheumatoid arthritis and multiple sclerosis (e.g. see H.J.Dyke et al., Expert Opinion on Investigational Drugs, January 2002, 11(1), 1-13; C.Burnouf et al., Cun-ent Pharmaceutical Design, 2002, 8(14), 1255-1296; and A.M.Doherty, Current Opinion Chem. Biol., 1999, 3(4), 466-473; and refs cited therein). See e.g. A.M.Doherty, Current Opinion Chem. Biol., 1999, 3(4), 466-473 and refs cited therein for atopic dermatitis use.

    [0209] PDE4 inhibitors have been suggested as having analgesic properties and thus being effective in the treatment of pain (A.Kumar et al., Indian J. Exp. Biol., 2000, 38(1), 26-30).

    [0210] In the invention, the treatment and/or prophylaxis can be of cognitive impairment e.g. cognitive impairment in a neurological disorder such as Alzheimer's disease. For example, the treatment and/or prophylaxis can comprise cognitive enhancement e.g. in a neurological disorder. See for example: H.T.Zhang et al. in: Psychopharmacology, June 2000,150(3), 311-316 and Neuropsychopharmacology, 2000, 23(2), 198-204; and T. Egawa et al., Japanese J. Pharmacol., 1997, 75(3), 275-81.

    [0211] PDE4 inhibitors such as rolipram have been suggested as having antidepressant properties (e.g. J. Zhu et al., CNS Drug Reviews, 2001, 7(4), 387-398; O'Donnell, Expert Opinion on Investigational Drugs, 2000, 9(3), 621-625; and H.T. Zhang et al., Neuropsychopharmacology, October 2002, 27(4), 587-595).

    Pharmaceutical compositions and dosing



    [0212] For use in medicine, the compounds of the present invention are usually administered as a pharmaceutical composition.

    [0213] The present invention therefore provides in a further aspect a pharmaceutical composition comprising a compound of formula (1) or a pharmaceutically acceptable salt thereof and one or more pharmaceutically acceptable carriers and/or excipients.

    [0214] The pharmaceutical composition can be for use in the treatment and/or prophylaxis of any of the conditions described herein.

    [0215] The invention also provides a method of preparing a pharmaceutical composition comprising a compound of formula (I), as herein defined, or a pharmaceutically acceptable salt thereof, and one or more pharmaceutically acceptable carriers and/or excipients,
    the method comprising mixing the compound or salt with the one or more pharmaceutically acceptable carriers and/or excipients.

    [0216] The invention also provides a pharmaceutical composition prepared by said method.

    [0217] The compounds of formula (I) and/or the pharmaceutical composition may be administered, for example, by oral, parenteral (e.g. intravenous, subcutaneous, or intramuscular), inhaled or nasal administration. Accordingly, the pharmaceutical composition is preferably suitable for oral, parenteral (e.g. intravenous, subcutaneous, or intramuscular), inhaled or nasal administration. More preferably, the pharmaceutical composition is suitable for inhaled or oral administration, e.g. to a mammal such as a human. Inhaled administration involves topical administration to the lung e.g. by aerosol or dry powder composition. Oral administration to a human is most preferred.

    [0218] A pharmaceutical composition suitable for oral administration can be liquid or solid; for example it can be a syrup, suspension or emulsion, a tablet, a capsule or a lozenge.

    [0219] A liquid formulation will generally consist of a suspension or solution of the compound or pharmaceutically acceptable salt in a suitable pharmaceutically acceptable liquid carrier(s), for example an aqueous solvent such as water, ethanol or glycerine, or a non-aqueous solvent, such as polyethylene glycol or an oil. The formulation may also contain a suspending agent, preservative, flavouring and/or colouring agent.

    [0220] In one preferable embodiment, the pharmaceutical composition is in unit dose form such as a tablet or capsule for oral administration, e.g. for oral administration to a human.

    [0221] A pharmaceutical composition suitable for oral administration being a tablet can comprise one or more pharmaceutically acceptable carriers and/or excipients suitable for preparing tablet formulations. The carrier can for example be or include lactose, cellulose (for example microcrystalline cellulose), or mannitol. The tablet can also or instead contain one or more pharmaceutically acceptable excipients, for example a binding agent such as hydroxypropylmethylcellulose or povidone (polyvinylpyrollidone), a lubricant e.g. an alkaline earth metal stearate such as magnesium stearate, and/or a tablet disintegrant such as sodium starch glycollate, croscarmellose sodium, or crospovidone (cross-linked polyvinylpyrollidone). The pharmaceutical composition being a tablet can be prepared by a method comprising the steps of: (i) mixing the compound of formula (I), as herein defined, or a pharmaceutically acceptable salt thereof, with the one or more pharmaceutically acceptable carriers and/or excipients, (ii) compressing the resulting mixture (which is usually in powder form) into tablets, and (iii) optionally coating the tablet with a tablet film-coating material.

    [0222] A pharmaceutical composition suitable for oral administration being a capsule can be prepared using encapsulation procedures. For example, pellets or powder containing the active ingredient can be prepared using a suitable pharmaceutically acceptable carrier and then filled into a hard gelatin capsule. Alternatively, a dispersion or suspension can be prepared using any suitable pharmaceutically acceptable carrier, for example an aqueous gum or an oil and the dispersion or suspension then filled into a soft gelatin capsule.

    [0223] A parenteral composition can comprise a solution or suspension of the compound or pharmaceutically acceptable salt in a sterile aqueous carrier or parenterally acceptable oil. Alternatively, the solution can be lyophilised; the lyophilised parenteral pharmaceutical composition can be reconstituted with a suitable solvent just prior to administration.

    [0224] Compositions for nasal or inhaled administration may conveniently be formulated as aerosols, drops, gels or dry powders.

    [0225] Aerosol formulations, e.g. for inhaled administration, can comprise a solution or fine suspension of the active substance in a pharmaceutically acceptable aqueous or non-aqueous solvent. Aerosol formulations can be presented in single or multidose quantities in sterile form in a sealed container, which can take the form of a cartridge or refill for use with an atomising device or inhaler. Alternatively the sealed container may be a unitary dispensing device such as a single dose nasal inhaler or an aerosol dispenser fitted with a metering valve (metered dose inhaler) which is intended for disposal once the contents of the container have been exhausted.

    [0226] Where the dosage form comprises an aerosol dispenser, it preferably contains a suitable propellant under pressure such as compressed air, carbon dioxide, or an organic propellant such as a chlorofluorocarbon (CFC) or hydrofluorocarbon (HFC). Suitable CFC propellants include dichlorodifluoromethane, trichlorofluoromethane and dichlorotetrafluoroethane. Suitable HFC propellants include 1,1,1,2,3,3,3-heptafluoropropane and 1,1,1,2-tetrafluoroethane. The aerosol dosage forms can also take the form of a pump-atomiser.

    Particle size reduction of compound of formula (I) or salt thereof



    [0227] For pharmaceutical compositions suitable and/or adapted for inhaled administration, it is preferred that the compound or salt of formula (I) is in a particle-size-reduced form, and more preferably the size-reduced form is obtained or obtainable by micronisation. Micronisation usually involves subjecting the compound/salt to collisional and abrasional forces in a fast-flowing circular or spiral/vortex-shaped airstream often including a cyclone component. The preferable particle size (e.g. D50 value) of the size-reduced (e.g. micronised) compound or salt is about 0.5 to about 10 microns, e.g. about 1 to about 5 microns (e.g. as measured using laser diffraction). For example, it is preferable for the compound or salt of formula (I) to have a particle size defined by: a D10 of about 0.3 to about 3 microns (e.g. about 1 micron), and/or a D50 of about 1 to about 5 microns (e.g. about 2-5 or about 2-3 microns), and/or a D90 of about 2 to about 20 microns or about 3 to about 10 microns (e.g. about 5-8 or about 5-6 microns); for example as measured using laser diffraction. The laser diffraction measurement can use a dry method (suspension of compound/salt in airflow crosses laser beam) or a wet method [suspension of compound/salt in liquid dispersing medium, such as isooctane or (e.g. if compound soluble in isooctane) 0.1% Tween 80 in water, crosses laser beam]. With laser diffraction, particle size is preferably calculated using the Fraunhofer calculation; and/or preferably a Malvern Mastersizer or Sympatec apparatus is used for measurement.

    [0228] An illustrative non-limiting example of a small-scale micronisation process is now given:

    Micronisation Example



    [0229] 
    • Purpose: To micronize a compound of formula (I) or a salt thereof - in particular one of the Examples of the invention (described hereinafter) - usually in an amount of approximately 600-1000 mg, using a Jetpharma MC1 micronizer.
    • The parent (unmicronised) and micronised materials are analyzed for particle size by laser diffraction and crystallinity by PXRD.

    Equipment and material



    [0230] 
    Equipment/material Description and specification
    Jetpharma MC1 Micronizer Nitrogen supply: Air thank with 275psi
      rate tubing
    Analytical balance Sartorius Analytical
    Top loader balance Mettler PM400
    Digital Caliper VWR Electronic caliper
    Vibrational spatula Auto-spat Dispenser
    Materials to be micronised (not yet performed)


    [0231] The Jetpharma MC1 Micronizer comprises a horizontal disc-shaped milling housing having: a tubular compound inlet (e.g. angled at ca. 30degrees to the horizontal) for entry of a suspension of unmicronised compound of formula (I) or salt in an gasflow, a separate gas inlet for entry of gases, a gas outlet for exit of gases, and a collection vessel for collecting micronised material. The milling housing has two chambers: an outer annular chamber in gaseous connection with the gas inlet the chamber being for receiving pressurised gas (e.g. air or nitrogen), an disc-shaped inner milling chamber within and coaxial with the outer chamber for micronising the input compound / salt, the two chambers being separated by an annular wall. The annular wall (ring R) has a plurality of narrow-bored holes connecting the inner and outer chambers and circumferentially-spaced-apart around the annular wall. The holes open into the inner chamber directed at an angle (directed part-way between radially and tangentially), and in use act as nozzles directing pressurised gas at high velocity from the outer chamber into the inner chamber and in an inwardly-spiral path (vortex) around the inner chamber (cyclone). The compound inlet is is gaseous communication with the inner chamber via a nozzle directed tangentially to the inner chamber, within and near to the annular wall. Upper and lower broad-diameter exit vents in the central axis of the the inner milling chamber connect to (a) (lower exit) the collection vessel which has no air outlet, and (b) (upper exit) the gas outlet which leads to a collection bag, filter and a gas exhaust. Inside the tubular compound inlet and longitudinally-movable within it is positioned a venturi inlet (V) for entry of gases. The compound inlet also has a bifurcation connecting to an upwardly-directed material inlet port for inputting material.

    [0232] In use, the narrow head of the venturi inlet (V) is preferably positioned below and slightly forward of the material inlet port so that when the venturi delivers pressurised gas (eg air or nitrogen) the feed material is sucked into the gasstream thorough the compound inlet and accelerates it into the inner milling chamber tangentially at a subsonic speed. Inside the milling chamber the material is further accelerated to a supersonic speed by the hole/nozzle system around the ring (R ) (annular wall) of the milling chamber. The nozzles are slightly angled so that the acceleration pattern of the material is in the form of an inwardly-directed vortex or cyclone. The material inside the milling chamber circulates rapidly and particle collisions occur during the process, causing larger particles to fracture into smaller ones. "Centrifugal" acceleration in the vortex causes the larger particles to remain at the periphery of the inner chamber while progressively smaller particles move closer to the center until they exit the milling chamber, generally through the lower exit, at low pressure and low velocity. The particles that exit the milling chamber are heavier than air and settle downward thorugh the lower exit into the collection vessel, while the exhaust gas rises (together with a miinority of small particles ofmicronised material) and escapes into the atmosphere at low pressure and low velocity.

    Procedure:



    [0233] The micronizer is assembled. The venturi protrusion distance from input port is adjusted to 1.0cm respectively (e.g. so that the narrow head of the venturi inlet is positioned below and slightly forward of the material inlet port) and is measured with a micro-caliper to make sure that it is inserted correctly. The ring (R ) and venturi (V) pressures are adjusted according to the values specified in the experimental design (refer to experimental section below) by adjusting the valves on the pressure gauges on the micronizer. The setup is checked for leakage by observing if there is any fluctuation in the reading of the pressure gauges.

    [0234] Note that the venturi (V) pressure is kept at least 2 bars greater than the ring (R ) pressure to prevent regurgitation of material, e.g. outwardly from the material inlet port.

    [0235] Balance performance is checked with calibration weights. Specified amount of the parent material (see section on experimental run) is weighed into a plastic weigh boat. The material is then fed into the micronizer using a vibrational spatula (e.g. V-shaped in cross-section) at a specified feed rate. The material feeding time and equipment pressures are monitored during the micronization process.

    [0236] Upon completion of the micronising run, the nitrogen supply is shut off and the collection bag is tapped to allow particles to settle into the recovery / collection vessel at the bottom of the micronizer. The collection bag is removed and set aside. The micronised powder in the recovery vessel (collection vessel) and the cyclone (above the recovery vessel) are collected separately into different weighed+labelled collection vials. The weight of the micronised material is recorded. The micronizer is disassembled and residual PDE4 compound on the micronizer inner surface is rinsed with 70/30 isopropyl alcohol / water and collected into a flask. The micronizer is then thoroughly cleaned by rinsing and wiping with suitable solvent and dried before subsequent runs are performed.

    Preferred Experimental Parameters



    [0237] 
    Balance(s) Used: Sartorius analytical
    Venturi outlet insertion depth: 10.0 mm
    Procedure no.Material input amount (g)Venturi (V) / ring (R) Pressure (bar)Intended feed-rateTime needed to feed material (min+sec)Actual feed-rate (g/min)
    1 0.8795g V=10bar 200 mg/min 4 min 51 sec 0.181 g/min
        R= 6 bar    
    2 0.9075g V= 8 bar 200 mg/min 4 min 43 sec 192 mg/min
        R= 5.5 bar    


    [0238] The above preferred or optional parameters can be varied using the skilled person's knowledge.

    Yield calculations



    [0239] 



    [0240] In general, very approximately 50-75 % yields are achievable using this method.

    Dry powder inhalable compositions



    [0241] For pharmaceutical compositions suitable and/or adapted for inhaled administration, it is preferred that the pharmaceutical composition is a dry powder inhalable composition. Such a composition can comprise a powder base such as lactose or starch, the compound of formula (I) or salt thereof (preferably in particle-size-reduced form, e.g. in micronised form), and optionally a performance modifier such as L-leucine, mannitol, trehalose and/or magnesium stearate. Preferably, the dry powder inhalable composition comprises a dry powder blend of lactose and the compound of formula (I) or salt thereof. The lactose is preferably lactose hydrate e.g. lactose monohydrate and/or is preferably inhalation-grade and/or fine-grade lactose. Preferably, the particle size of the lactose is defined by 90% or more (by weight or by volume) of the lactose particles being less than 1000 microns (micrometres) (e.g. 10-1000 microns e.g. 30-1000 microns) in diameter, and/or 50% or more of the lactose particles being less than 500 microns (e.g. 10-500 microns) in diameter. More preferably, the particle size of the lactose is defined by 90% or more of the lactose particles being less than 300 microns (e.g. 10-300 microns e.g. 50-300 microns) in diameter, and/or 50% or more of the lactose particles being less than 100 microns in diameter. Optionally, the particle size of the lactose is defined by 90% or more of the lactose particles being less than 100-200 microns in diameter, and/or 50% or more of the lactose particles being less than 40-70 microns in diameter. Most importantly, it is preferable that about 3 to about 30% (e.g. about 10%) (by weight or by volume) of the particles are less than 50 microns or less than 20 microns in diameter. For example, without limitation, a suitable inhalation-grade lactose is E9334 lactose (10% fines) (Borculo Domo Ingredients, Hanzeplein 25, 8017 JD Zwolle, Netherlands).

    [0242] In the dry powder inhalable composition, preferably, the compound of formula (I) or salt thereof is present in about 0.1% to about 70% (e.g. about 1% to about 50%, e.g. about 5% to about 40%, e.g. about 20 to about 30%) by weight of the composition.

    [0243] An illustrative non-limiting example of a dry powder inhalable composition follows:

    Dry Powder Formulation Example - Dry powder Lactose Blend Preparation



    [0244] Using a size-reduced e.g. micronised form of the compound of formula (I) or salt thereof (e.g. as prepared in the Micronisatrion Example above), the dry powder blend is prepared by mixing the required amount of the compound/salt (e.g. 10 mg, 1% w/w) with inhalation-grade lactose containing 10% fines (e.g. 990 mg, 99% w/w) in a Teflon™ (polytetrafluoroethene) pot in a Mikro-dismembrator ball-mill (but without a ball bearing) at ¾ speed (ca. 2000-2500 rpm) for about 4 hours at each blend concentration. The Mikro-dismembrator (available from B. Braun Biotech International, Schwarzenberger Weg 73-79, D-34212 Melsungen, Germany; www.bbraunbiotech.com) comprises a base with an upwardly-projecting and sidewardly-vibratable arm to which is attached the Teflon ™ pot. The vibration of the arm achieves blending.
    Other blends: 10%w/w compound/salt (50 mg)

    [0245] Serial dilution of the 1% w/w blend can achieve e.g. 0.1% and 0.3% w/w blends.

    Dry powder inhalation devices



    [0246] Optionally, in particular for dry powder inhalable compositions, a pharmaceutical composition for inhaled administration can be incorporated into a plurality of sealed dose containers (e.g. containing the dry powder composition) mounted longitudinally in a strip or ribbon inside a suitable inhalation device. The container is rupturable or peel-openable on demand and the dose, e.g. of the dry powder composition, can be administered by inhalation via a device such as the DISKUS device, marketed by GlaxoSmithKline. The DISKUS inhalation device is usually substantially as described in GB 2,242,134 A, and in such device at least one container for the pharmaceutical composition in powder form (the at least one container preferably being a plurality of sealed dose containers mounted longitudinally in a strip or ribbon) is defined between two members peelably secured to one another; the device comprises: means defining an opening station for the said at least one container; means for peeling the members apart at the opening station to open the container; and an outlet, communicating with the opened container, through which a user can inhale the pharmaceutical composition in powder form from the opened container.

    Unit dose form and dosing regimens



    [0247] Preferably the composition is in unit dose form such as a tablet or capsule for oral administration, e.g. for oral administration to a human.

    [0248] In the pharmaceutical composition, a or each dosage unit for oral or parenteral administration preferably contains from 0.01 to 3000 mg, more preferably 0.5 to 1000 mg, of a compound of the formula (I) or a pharmaceutically acceptable salt thereof, calculated as the free base. A or each dosage unit for nasal or inhaled administration preferably contains from 0.001 to 50 mg, more preferably 0.01 to 5 mg, of a compound of the formula (I) or a pharmaceutically acceptable salt thereof, calculated as the free base.

    [0249] A pharmaceutically acceptable compound or salt of the invention is preferably administered to a mammal (e.g. human) in a daily oral or parenteral dose of 0.001 mg to 50 mg per kg body weight per day (mg/kg/day), for example 0.01 to 20 mg/kg/day or 0.03 to 10 mg/kg/day or 0.1 to 2 mg/kg/day, of the compound of the formula (I) or a pharmaceutically acceptable salt thereof, calculated as the free base.

    [0250] A pharmaceutically acceptable compound or salt of the invention is preferably administered to a mammal (e.g. human) in a daily nasal or inhaled dose of: 0.0001 to 5 mg/kg/day or 0.0001 to 1 mg/kg/day, e.g. 0.001 to 1 mg/kg/day or 0.001 to 0.3 mg/kg/day or 0.001 to 0.1 mg/kg/day or 0.005 to 0.3 mg/kg/day, of the compound of the formula (I) or a pharmaceutically acceptable salt thereof, calculated as the free base.

    [0251] The pharmaceutically acceptable compounds or salts of the invention is preferably administered in a daily dose (for an adult patient) of, for example, an oral or parenteral dose of 0.01 mg to 3000 mg per day or 0.5 to 1000 mg per day e.g. 2 to 500 mg per day, or a nasal or inhaled dose of 0.001 to 300 mg per day or 0.001 to 50 mg per day or 0.01 to 30 mg per day or 0.01 to 5 mg per day or 0.02 to 2 mg per day, of the compound of the formula (I) or a pharmaceutically acceptable salt thereof, calculated as the free base.

    Combinations



    [0252] The compounds, salts and/or pharmaceutical compositions according to the invention may also be used in combination with another therapeutically active agent, for example, a β2 adrenoreceptor agonist, an anti-histamine, an anti-allergic or an anti-inflammatory agent.

    [0253] The invention thus provides, in a further aspect, a combination comprising a compound of formula (I) or a pharmaceutically acceptable salt thereof together with another therapeutically active agent, for example, a β2-adrenoreceptor agonist, an anti-histamine, an anti-allergic, an anti-inflammatory agent or an antiinfective agent.

    [0254] Preferably, the β2-adrenoreceptor agonist is salmeterol (eg as racemate or a single enantiomer such as the R-enantiomer), salbutamol, formoterol, salmefamol, fenoterol or terbutaline, or a salt thereof (e.g. pharmaceutically acceptable salt thereof), for example the xinafoate salt of salmeterol, the sulphate salt or free base of salbutamol or the fumarate salt of formoterol. Long-acting β2-adrenoreceptor agonists are preferred, especially those having a therapeutic effect over a 12-24 hour period such as salmeterol or formoterol. Preferably, the β2-adrenoreceptor agonist is for inhaled administration, e.g. once per day and/or for simultaneous inhaled administration; and more preferably the β2-adrenoreceptor agonist is in particle-size-reduced form e.g. as defined herein. Preferably, the β2-adrenoreceptor agonist combination is for treatment and/or prophylaxis of COPD or asthma. Salmeterol or a pharmaceutically acceptable salt thereof, e.g. salmeterol xinofoate, is preferably administered to humans at an inhaled dose of 25 to 50 micrograms twice per day (measured as the free base). The combination with a β2-adrenoreceptor agonist can be as described in WO 00/12078.

    [0255] Preferred long acting β2-adrenoreceptor agonists include those described in WO 02/066422A, WO 03/024439, WO 02/070490 and WO 02/076933.

    [0256] Especially preferred long-acting β2-adrenoreceptor agonists include compounds of formula (X) (described in WO 02/066422) (note that the R groups therein are defined independently of the corresponding R groups of formula (I)):

    or a salt or solvate thereof, wherein in formula (X):

    m is an integer of from 2 to 8;

    n is an integer of from 3 to 11,
    with the proviso that m + n is 5 to 19,
    R11 is -XSO2NR16R17 wherein X is -(CH2)p- or C2-6 alkenylene;
    R16 and R17 are independently selected from hydrogen, C1-6alkyl, C3-7cycloalkyl, C(O)NR18R19, phenyl, and phenyl (C1-4alkyl)-,
    or R16 and R17, together with the nitrogen to which they are bonded, form a 5-, 6-, or 7-membered nitrogen containing ring, and R16 and R17 are each optionally substituted by one or two groups selected from halo, C1-6alkyl, C1-6haloalkyl, C1-6alkoxy, hydroxy-substituted C1-6alkoxy, -CO2R18, -SO2NR18R19, -CONR18R19, -NR18C(O)R19, or a 5-, 6-or 7-membered heterocylic ring;
    R18 and R19 are independently selected from hydrogen, C1-6alkyl,
    C3-6cycloalkyl, phenyl, and phenyl (C1-4alkyl)-; and
    p is an integer of from 0 to 6, preferably from 0 to 4;
    R12 and R13 are independently selected from hydrogen, C1-6alkyl, C1-6alkoxy, halo, phenyl, and C1-6haloalkyl; and
    R14 and R15 are independently selected from hydrogen and C1-4alkyl with the proviso that the total number of carbon atoms in R14 and R15 is not more than 4.



    [0257] Preferred β2-adrenoreceptor agonists disclosed in WO 02/066422 include:

    3-(4- {[6-({(2R)-2-hydroxy-2-[4-hydroxy-3-(hydroxymethyl)-phenyl]ethyl}amino)hexyl]oxy}butyl)benzenesulfonamide and

    3-(3-{[7-({(2R)-2-hydroxy-2-[4-hydroxy-3-hydroxymethyl)phenyl]ethyl}-amino)heptyl]oxy}propyl)benzenesulfonamide.



    [0258] A preferred β2-adrenoreceptor agonist disclosed in WO 03/024439 is: 4-{(1R)-2-[(6-{2-[(2,6-dichlorobenzyl)oxy]ethoxy}hexyl)amino]-1-hydroxyethyl}-2-(hydroxymethyl)phenol.

    [0259] A combination of a compound of formula (I) or salt together with an anti-histamine is preferably for oral administration (e.g, as a combined composition such as a combined tablet), and can be for treatment and/or prophylaxis of allergic rhinitis. Examples of antihistamines include methapyrilene, or H1 antagonists such as cetirizine, loratadine (e.g. Clarityn™), desloratadine (e.g. Clarinex™) or fexofenadine (e.g. Allegra™).

    [0260] The invention also provides, in a further aspect, a combination comprising a compound of formula (I) or a pharmaceutically acceptable salt thereof together with an anticholinergic compound, e.g. a muscarinic (M) receptor antagonist in particular an M1, M2, M1/M2, or M3 receptor antagonist, more preferably a M3 receptor antagonist, still more preferably a M3 receptor antagonist which selectively antagonises (e.g. antagonises 10 times or more strongly) the M3 receptor over the M1 and/or M2 receptor. For combinations of anticholinergic compounds / muscarinic (M) receptor antagonists with PDE4 inhibitors, see for example WO 03/011274 A2 and WO 02/069945 A2 / US 2002/0193393 A1 and US 2002/052312 A1, and some or all of these publications give examples of anticholinergic compounds / muscarinic receptor antagonists which may be used with the compounds of formula (I) or salts, and/or suitable pharmaceutical compositions. For example, the muscarinic receptor antagonist can comprise or be an ipratropium salt (e.g. ipratropium bromide), an oxitropium salt (e.g. oxitropium bromide), or more preferably a tiotropium salt (e.g. tiotropium bromide); see e.g. EP 418 716 A1 for tiotropium.

    [0261] The anticholinergic compound or muscarinic (M) receptor antagonist, e.g. M3 receptor antagonist, is preferably for inhaled administration, more preferably in particle-size-reduced form e.g. as defined herein. More preferably, both the muscarinic (M) receptor antagonist and the compound of formula (I) or the pharmaceutically acceptable salt thereof are for inhaled administration. Preferably, the anticholinergic compound or muscarinic receptor antagonist and the compound of formula (I) or salt are for simultaneous administration. The muscarinic receptor antagonist combination is preferably for treatment and/or prophylaxis of COPD.

    [0262] Other suitable combinations include, for example, a combination comprising a compound of formula (I) or a pharmaceutically acceptable salt thereof together with another anti-inflammatory agent such as an anti-inflammatory corticosteroid; or a non-steroidal anti-inflammatory drug (NSAID) such as a leukotriene antagonist (e.g. montelukast), an iNOS inhibitor, a tryptase inhibitor, an elastase inhibitor, a beta-2 integrin antagonist, an adenosine 2a agonist, a CCR3 antagonist, or a 5-lipoxogenase inhibitor; or an antiinfective agent (eg. an antibiotic or an antiviral). An iNOS inhibitor is preferably for oral administration. Suitable iNOS inhibitors (inducible nitric oxide synthase inhibitors) include those disclosed in WO 93/13055, WO 98/30537, WO 02/50021, WO 95/34534 and WO 99/62875. Suitable CCR3 inhibitors include those disclosed in WO 02/26722.

    [0263] In a combination comprising a compound of formula (I) or a pharmaceutically acceptable salt thereof together with an anti-inflammatory corticosteroid (which is preferably for treatment and/or prophylaxis of asthma, COPD or allergic rhinitis), then preferably the anti-inflammatory corticosteroid is fluticasone, fluticasone propionate (e.g. see US patent 4,335,121), beclomethasone, beclomethasone 17-propionate ester, beclomethasone 17,21-dipropionate ester, dexamethasone or an ester thereof, mometasone or an ester thereof, ciclesonide, budesonide, flunisolide, or a compound as described in WO 02/12266 A1 (e.g. as claimed in any of claims 1 to 22 therein), or a pharmaceutically acceptable salt of any of the above. If the anti-inflammatory corticosteroid is a compound as described in WO 02/12266 A1, then preferably it is Example 1 therein {which is 6α,9α-difluoro-17α-[(2-furanylcarbonyl)oxy]-11β-hydroxy-16α-methyl-3-oxo-androsta-1,4-diene-17β-carbothioic acid S-fluoromethyl ester} or Example 41 therein {which is 6α,9α-difluoro-11β-hydroxy-16α-methyl-17α-[(4-methyl-1,3-thiazole-5-carbonyl)oxy]-3-oxo-androsta-1,4-diene-17β-carbothioic acid S-fluoromethyl ester}, or a pharmaceutically acceptable salt thereof. The anti-inflammatory corticosteroid is preferably for intranasal or inhaled administration. Fluticasone propionate is preferred and is preferably for inhaled administration to a human either (a) at a dose of 250 micrograms once per day or (b) at a dose of 50 to 250 micrograms twice per day.

    [0264] Also provided is a combination comprising a compound of formula (I) or a pharmaceutically acceptable salt thereof together with β2-adrenoreceptor agonist and an anti-inflammatory corticosteroid, for example as described in WO 03/030939 A1. Preferably this combination is for treatment and/or prophylaxis of asthma, COPD or allergic rhinitis. The β2-adrenoreceptor agonist and/or the anti-inflammatory corticosteroid can be as described above and/or as described in WO 03/030939 A1. Most preferably, in this "triple" combination, the β2-adrenoreceptor agonist is salmeterol or a pharmaceutically acceptable salt thereof (e.g. salmeterol xinafoate) and the anti-inflammatory corticosteroid is fluticasone propionate.

    [0265] The combinations referred to above may conveniently be presented for use in the form of a pharmaceutical composition and thus a pharmaceutical composition comprising a combination as defined above together with one or more pharmaceutically acceptable carriers and/or excipients represent a further aspect of the invention.

    [0266] The individual compounds of such combinations may be administered either sequentially or simultaneously in separate or combined pharmaceutical composition(s).

    [0267] In one embodiment, the combination as defined herein can be for simultaneous inhaled administration and is disposed in a combination inhalation device. Such a combination inhalation device is another aspect of the invention. Such a combination inhalation device can comprise a combined pharmaceutical composition for simultaneous inhaled administration (e.g. dry powder composition), the composition comprising all the individual compounds of the combination, and the composition being incorporated into a plurality of sealed dose containers mounted longitudinally in a strip or ribbon inside the inhalation device, the containers being rupturable or peel-openable on demand; for example such inhalation device can be substantially as described in GB 2,242,134 A (DISKUS™) and/or as described above. Alternatively, the combination inhalation device can be such that the individual compounds of the combination are administrable simultaneously but are stored separately (or wholly or partly stored separately for triple combinations), e.g. in separate pharmaceutical compositions, for example as described in PCT/EP03/00598 filed on 22 January 2003 and published as WO 03/061743 A1 (e.g. as described in the claims thereof e.g. claim 1).

    [0268] The invention also provides a method of preparing a combination as defined herein,
    the method comprising either
    1. (a) preparing a separate pharmaceutical composition for administration of the individual compounds of the combination either sequentially or simultaneously, or
    2. (b) preparing a combined pharmaceutical composition for administration of the individual compounds of the combination simultaneously,
    wherein the pharmaceutical composition comprises the combination together with one or more pharmaceutically acceptable carriers and/or excipients.

    [0269] The invention also provides a combination as defined herein, prepared by a method as defined herein.

    Biological Test Methods


    PDE 3, PDE 4B, PDE 4D, PDE 5, PDE 6 Primary assay methods



    [0270] The activity of the compounds can be measured in the assay methods shown below. Preferred compounds of the invention are selective PDE4 inhibitors, i.e. they inhibit PDE4 (e.g. PDE4B and/or PDE4D, preferably PDE4B) more strongly than they inhibit PDE3 and/or more strongly than they inhibit PDE5 and/or more strongly than they inhibit PDE6.

    PDE enzyme sources and literature references



    [0271] Human recombinant PDE4B, in particular the 2B splice variant thereof (HSPDE4B2B), is disclosed in WO 94/20079 and also M.M. McLaughlin et al., "A low Km, rolipram-sensitive, cAMP-specific phosphodiesterase from human brain: cloning and expression of cDNA, biochemical characterisation of recombinant protein, and tissue distribution of mRNA", J. Biol. Chem., 1993, 268, 6470-6476. Human recombinant PDE4B was expressed in the PDE-deficient yeast Saccharomyces cerevisiae strain GL62. 100,000 x g supernatant fractions of yeast cell lysates were used for PDE4B assays and inhibitor studies.

    [0272] Human recombinant PDE4D (HSPDE4D3A) is disclosed in P. A. Baecker et al., "Isolation of a cDNA encoding a human rolipram-sensitive cyclic AMP phoshodiesterase (PDE IVD)", Gene, 1994, 138, 253-256.

    [0273] Human recombinant PDE5 is disclosed in K. Loughney et al., "Isolation and characterisation of cDNAs encoding PDE5A, a human cGMP-binding, cGMP-specific 3',5'-cyclic nucleotide phosphodiesterase", Gene, 1998, 216, 139-147.

    [0274] PDE3 was purified from bovine aorta as described by H. Coste and P. Grondin, "Characterisation of a novel potent and specific inhibitor of type V phosphodiesterase", Biochem. Pharmacol., 1995, 50, 1577-1585.

    [0275] PDE6 was purified from bovine retina as described by: P. Catty and P. Deterre, "Activation and solubilization of the retinal cGMP-specific phosphodiesterase by limited proteolysis", Eur. J. Biochem., 1991, 199, 263-269; A. Tar et al. "Purification of bovine retinal cGMP phosphodiesterase", Methods in Enzymology, 1994, 238, 3-12; and/or D. Srivastava et al. "Effects of magnesium on cyclic GMP hydrolysis by the bovine retinal rod cyclic GMP phosphodiesterase", Biochem. J., 1995, 308, 653-658.

    Inhibition of PDE 3, PDE 4B, PDE 4D, PDE 5 or PDE 6 activity: radioactive Scintillation Proximity Assay (SPA)



    [0276] The ability of compounds to inhibit catalytic activity at PDE4B or 4D (human recombinant), PDE3 (from bovine aorta) or PDE5 (human recombinant) or PDE6 (from bovine retina) was determined by Scintillation Proximity Assay (SPA) in 96-well format. Test compounds (preferably as a solution in DMSO, e.g. about 2 microlitre (ul) volume of DMSO solution) were preincubated at ambient temperature (room temperature, e.g. 19-23°C) in Wallac Isoplates (code 1450-514) with PDE enzyme in 50mM Tris-HCl buffer pH 7.5 , 8.3mM MgCl2, 1.7mM EGTA, 0.05% (w/v) bovine serum albumin for 10-30 minutes (usually 30 minutes). The enzyme concentration was adjusted so that no more than 20% hydrolysis of the substrate defined below occurred in control wells without compound, during the incubation. For PDE3, PDE4B and PDE4D assays, [5',8-3H]Adenosine 3',5'-cyclic phosphate ( Amersham Pharmacia Biotech , code TRK.559; or Amersham Biosciences UK Ltd, Pollards Wood, Chalfont St Giles, Buckinghamshire HP8 4SP, UK) was added to give 0.05uCi per well and ∼ 10nM final concentration. For the PDE5 and PDE6 assay [8-3H]Guanosine 3',5'-cyclic phosphate ( Amersham Pharmacia Biotech, code TRK.392) was added to give 0.05uCi per well and - 36nM final concentration. Plates, preferably containing approx. 100 ul volume of assay mixture, were mixed on an orbital shaker for 5 minutes and incubated at ambient temperature for 1 hour. Phosphodiesterase SPA beads (Amersham Pharmacia Biotech, code RPNQ 0150) were added (∼1mg per well) to terminate the assay. Plates were sealed and shaken and allowed to stand at ambient temperature for 35 minutes to 1 hour (preferably 35 minutes) to allow the beads to settle. Bound radioactive product was measured using a WALLAC TRILUX 1450 Microbeta scintillation counter. For inhibition curves, 10 concentrations (1.5-M - 30uM) of each compound were assayed. Curves were analysed using ActivityBase and XLfit (ID Businesss Solutions Limited, 2 Ocean Court, Surrey Research Park, Guildford, Surrey GU2 7QB, United Kindgom) Results were expressed as pIC50 values.

    [0277] In an alternative to the above radioactive SPA assay, PDE4B or PDE4D inhibition can be measured in the following Fluorescence Polarisation (FP) assay:

    Inhibition of PDE4B or PDE4D activity: Fluorescence Polarisation (FP) assay



    [0278] The ability of compounds to inhibit catalytic activity at PDE4B (human recombinant) or PDE4D (human recombinant) was determined by IMAP Fluorescence Polarisation (FP) assay (IMAP Explorer kit, available from Molecular Devices Corporation, Sunnydale, CA, USA; Molecular Devices code: R8062) in 384-well format. The IMAP FP assay is able to measure PDE activity in an homogenous, non-radioactive assay format. The FP assay uses the ability of immobilised trivalent metal cations, coated onto nanoparticles (tiny beads), to bind the phosphate group of F1-AMP that is produced on the hydrolysis of fluorescein-labelled (F1) cyclic adenosine mono-phosphate (F1-cAMP) to the non-cyclic F1-AMP form. F1-cAMP does not bind. Binding of F1-AMP product to the beads (coated with the immobilised trivalent cations) slows the rotation of the bound F1-AMP and leads to an increase in the fluorescence polarisation ratio of parallel to perpendicular light. Inhibition of the PDE reduces/inhibits this signal increase.

    [0279] Test compounds (small volume, e.g. ca. 0.5 to 1 ul, preferably ca. 0.5 ul, of solution in DMSO) were preincubated at ambient temperature (room temperature, e.g. 19-23°C) in black 384-well microtitre plates (supplier: NUNC, code 262260) with PDE enzyme in 10mM Tris-HCl buffer pH 7.2, 10mM MgCl2, 0.1% (w/v) bovine serum albumin, and 0.05% NaN3 for 10-30 minutes. The enzyme level was set by experimentation so that reaction was linear throughout the incubation. Fluorescein adenosine 3',5'-cyclic phosphate (from Molecular Devices Corporation, Molecular Devices code: R7091) was added to give about 40nM final concentration (final assay volume usually ca. 20-40 ul, preferably ca. 20 ul). Plates were mixed on an orbital shaker for 10 seconds and incubated at ambient temperature for 40 minutes. IMAP binding reagent (as described above, from Molecular Devices Corporation, Molecular Devices code: R7207) was added (60ul of a 1 in 400 dilution in binding buffer of the kit stock solution) to terminate the assay. Plates were allowed to stand at ambient temperature for 1 hour. The Fluorescence Polarisation (FP) ratio of parallel to perpendicular light was measured using an Analyst plate reader (from Molecular Devices Corporation). For inhibition curves, 10 concentrations (1.5nM - 30uM) of each compound were assayed. Curves were analysed using ActivityBase and XLfit (ID Businesss Solutions Limited, 2 Ocean Court, Surrey Research Park, Guildford, Surrey GU2 7QB, United Kindgom). Results were expressed as pIC50 values.

    [0280] In the FP assay, all reagents were dispensed using Multidrop (available from Thermo Labsystems Oy, Ratastie 2, PO Box 100, Vantaa 01620, Finland).

    [0281] For a given PDE4 inhibitor, the PDE4B (or PDE4D) inhibition values measured using the SPA and FP assays can differ slightly. However, in a regression analysis of 100 test compounds, the pIC50 inhibition values measured using SPA and FP assays have been found generally to agree within 0.5 log units, for PDE4B and PDE4D (linear regression coefficient 0.966 for PDE4B and 0.971 for PDE4D; David R.Mobbs et al., "Comparison of the IMAP Fluorescence Polarisation Assay with the Scintillation Proximity Assay for Phosphodiesterase Activity", poster to be presented at 2003 Molecular Devices UK & Europe User Meeting, 2nd October 2003, Down Hall, Harlow, Essex, United Kingdom).

    [0282] Biological Data obtained for some of the Examples (PDE4B inhibitory activity, either as one reading or as an average of ca. 2-6 readings) are as follows, based on current measurements only. In each of the SPA and FP assays, absolute accuracy of measurement is not possible, and the readings given are accurate only up to about ± 0.5 of a log unit, depending on the number of readings made and averaged:
    Example numberPDE4B pIC50
    (± about 0.5)
    6 8.1
    10 8.2
    12 (Reference Example) 7.9
    14 7.6
    23 8.1
    24 8.2
    35 7.5
    42 8.3
    Examples 43, 48, and 61; Reference Examples 7, 60, and 64 6.6 to 7.2
    Examples 2, 17, 34, 38, 39, 44, 50, 59, 62, 63, 66, 71, 76, 77 and 84; and Reference Example 26 7.5 to 9.1
    Many, but not all, of the Examples have been tested for PDE4B inhibition. Of the Examples tested for PDE4B inhibition, some were tested by the radioactive SPA assay, some were tested by the FP assay.

    [0283] Most or substantially all of Examples 1-45, 47-55, 57-81, 83 and 84 have PDE4B inhibitory activities in the range of pIC50 = about 6 (± about 0.5) to about 9.1 (± 0.5).

    [0284] The Examples wherein R3 = cyclohexyl (NHR3 or NR3R3a = sub-formula (c)), tetrahydro-2H-pyran-4-yl (NHR3 or NR3R3a = group (h)), or certain other types of substituted cyclohexyl or certain heterocycles, or Examples wherein NHR3 or NR3R3a = sub-formula (s), usually or often (based on data for R1 = ethyl) have a higher level of selectivity for PDE4B over PDE5, as measured in the above enzyme inhibition assays, compared to the selectivities of comparable Examples wherein R3 = cyclopropyl (NHR3 or NR3R3a = sub-formula (b)).

    [0285] Emesis: Some known PDE4 inhibitors can cause emesis and/or nausea to greater or lesser extents (e.g. see Z. Huang et al., Current Opinion in Chemical Biology, 2001, 5: 432-438, see especially pages 433-434 and refs cited therein). Therefore, it would be preferable, but not essential, if a particular PDE4 inhibitory compound or salt of the invention were to cause only limited or manageable emetic side-effects. Emetic side-effects can for example be measured by the emetogenic potential of the compound or salt when administered to ferrets; for example one can measure the time to onset, extent, frequency and/or duration of vomiting, retching and/or writhing in ferrets after oral or parenteral administration of the compound or salt. See for example In vivo Assay 4 hereinafter for a measurement method for anti-inflammatory effect, emetic side-effects and therapeutic index (TI) in the ferret. See also for example A. Robichaud et al., "Emesis induced by inhibitors of [PDE IV] in the ferret", Neuropharmacology, 1999, 38, 289-297, erratum Neuropharmacology, 2001, 40, 465-465. However, optionally, emetic side-effects and therapeutic index (TI) in rats can be conveniently measured by monitoring the pica feeding behaviour of rats after administration of the compound or salt of the invention (see In Vivo Assay 2 below).

    [0286] Other side effects: Some known PDE4 inhibitors can cause other side effects such as headache and other central nervous sytem (CNS-) mediated side effects; and/or gastrointestinal (GI) tract disturbances. Therefore, it would be preferable but not essential if a particular PDE4 inhibitory compound or salt of the invention were to cause only limited or manageable side-effects in one or more of these side-effect categories.

    In Vivo Biological Assays



    [0287] The in vitro enzymatic PDE4B inhibition assay described above should be regarded as being the primary test of biological activity. However, additional in vivo biological tests, which are optional and which are not an essential measure of efficacy or side-effects, are described below.

    In Vivo Assay 1. LPS-induced pulmonary neutrophilia in rats: effect of orally administered PDE4 inhibitors



    [0288] Pulmonary neutrophil influx has been shown to be a significant component to the family of pulmonary diseases like chronic obstructive pulmonary disease (COPD) which can involve chronic bronchitis and/or emphysema (G.F. Filley, Chest. 2000; 117(5); 251s-260s). The purpose of this neutrophilia model is to study the potentially anti-inflammatory effects in vivo of orally administered PDE4 inhibitors on neutrophilia induced by inhalation of aerosolized lipopolysaccharide (LPS), modelling the neutrophil inflammatory component(s) of COPD. See the literature section below for scientific background.

    [0289] Male Lewis rats (Charles River, Raleigh, NC, USA) weighing approximately 300-400 grams are pretreated with either (a) test compound suspended in 0.5% methylcellulose (obtainable from Sigma-Aldrich, St Louis, MO, USA) in water or (b) vehicle only, delivered orally in a dose volume of 10 ml/kg. Generally, dose response curves are generated using the following doses of PDE4 inhibitors: 10.0, 2.0, 0.4, 0.08 and 0.016 mg/kg. Thirty minutes following pretreatment, the rats are exposed to aerosolized LPS (Serotype E. Coli 026:B6 prepared by trichloroacetic acid extraction, obtainable from Sigma-Aldrich, St Louis, MO, USA), generated from a nebulizer containing a 100 µg/ml LPS solution. Rats are exposed to the LPS aerosol at a rate of 4 L/min for 20 minutes. LPS exposure is carried out in a closed chamber with internal dimensions of 45 cm length x 24 cm width x 20 cm height. The nebulizer and exposure chamber are contained in a certified fume hood. At 4 hours-post LPS exposure the rats are euthanized by overdose with pentobarbital at 90 mg/kg, administered intraperitoneally. Bronchoalveolar lavage (BAL) is performed through a 14 gauge blunt needle into the exposed trachea. Five, 5 ml washes are performed to collect a total of 25 ml of BAL fluid. Total cell counts and leukocyte differentials are performed on BAL fluid in order to calculate neutrophil influx into the lung. Percent neutrophil inhibition at each dose (cf. vehicle) is calculated and a variable slope, sigmoidal dose-response curve is generated, usually using Prism Graph-Pad. The dose-response curve is used to calculate an ED50 value (in mg per kg of body weight) for inhibition by the PDE4 inhibitor of the LPS-induced neutrophilia.

    [0290] Results: Based on current measurements, the compounds of Examples 14, 17, 23, 35 and 38, administered orally in the above procedure, exhibited neutrophilia-inhibition ED50 values in the range of about 0.03 mg/kg to about 1 mg/kg, subject to testing inaccuracies.

    [0291] Alternative method and results: In an alternative embodiment of the procedure, a single oral dose of 10 mg/kg or 1 mg/kg of the PDE4 inhibitor (or vehicle) is administered to the rats, and percent neutrophil inhibition is calculated and reported for that specific dose. In this embodiment, based on current measurements, the compounds of Examples 2, 14, 23 and 38, administered orally in this alternative procedure at a single dose of 10 mg/kg, exhibited percent neutrophilia-inhibition in the range of about 74% to about 86%, subject to testing inaccuracies.

    Literature:



    [0292] Filley G.F. Comparison of the structural and inflammatory features of COPD and asthma. Chest. 2000; 117(5) 251s-260s.

    [0293] Howell RE, Jenkins LP, Fielding LE, and Grimes D. Inhibition of antigen-induced pulmonary eosinophilia and neutrophilia by selective inhibitors of phosphodiesterase types 3 and 4 in brown Norway rats. Pulmonary Pharmacology. 1995; 8: 83-89.

    [0294] Spond J, Chapman R, Fine J, Jones H, Kreutner W, Kung TT, Minnicozzi M. Comparison of PDE 4 inhibitors, Rolipram and SB 207499 (Ariflo™), in a rat model of pulmonary neutrophilia. Pulmonary Pharmacology and Therapeutics. 2001; 14: 157-164.

    [0295] Underwood DC, Osborn RR, Bochnowicz S, Webb EF, Rieman DJ, Lee JC, Romanic AM, Adams JL, Hay DWP, and Griswold DE. SB 239063, a p38 MAPK inhibitor, reduces neutrophilia, inflammatory cytokines, MMP-9, and fibrosis in lung. Am J Physiol Lung Cell Mol Physiol. 2000; 279: L895-L902.

    In Vivo Assay 2. Rat Pica Model of emesis



    [0296] Background: Selective PDE4 inhibitors have been shown to inhibit inflammation in various in vitro and in vivo models by increasing intracellular levels of cAMP of many immune cells (e.g. lymphocytes, monocytes). However, a side effect of some PDE4 inhibitors in many species is emesis. Because many rat models of inflammation are well characterized, they have been used in procedures (see e.g. In Vivo Assay 1 above) to show beneficial anti-inflammatory effects of PDE 4 inhibitors. However rats have no emetic response (they have no vomit reflex), so that the relationship between beneficial anti-inflammatory effects of PDE 4 inhibitors and emesis is difficult to study directly in rats.

    [0297] However, in 1991, Takeda et al. (see Literature section below) demonstrated that the pica feeding response is analogous to emesis in rats. Pica feeding is a behavioural response to illness in rats wherein rats eat non-nutritive substances such as earth or in particular clay (e.g. kaolin) which may help to absorb toxins. Pica feeding can be induced by motion and chemicals (especially chemicals which are emetic in humans), and can be inhibited pharmacologically with drugs that inhibit emesis in humans. The Rat Pica Model, In Vivo Assay 2, can determine the level of pica response of rats to PDE 4 inhibition at pharmacologically relevant doses in parallel to in vivo anti-inflammatory Assays in (a separate set of) rats (e.g. In Vivo Assay 1 above). Anti-inflammatory and pica assays in the same species together can provide data on the "therapeutic index" (TI) in the rat of the compounds/salts of the invention. The Rat TI can for example be calculated as the ratio of a) the potentially-emetic Pica Response ED50 dose from Assay 2 to b) the rat anti-inflammatory ED50 dose (e.g. measured by rat neutrophilia-inhibition in eg In Vivo Assay 1), with larger TI ratios possibly indicating lower emesis at many anti-inflammatory doses. This might allow a choice of a non-emetic or minimal-emetic pharmaceutical dose of the compounds or salts of the invention which has an anti-inflammatory effect. It is recognised however that achieving a low-emetic PDE4 inhibitory compound is not essential to the invention.

    [0298] Procedure: On the first day of the experiment, the rats are housed individually in cages without bedding or "enrichment". The rats are kept off of the cage floor by a wire screen. Pre-weighed food cups containing standard rat chow and clay pellets are placed in the cage. The clay pellets, obtainable from Languna Clay Co, City of Industry, CA, USA, are the same size and shape as the food pellets. The rats are acclimated to the clay for 72 hours, during which time the cups and food and clay debris from the cage are weighed daily on an electronic balance capable of measuring to the nearest 0.1 grams. By the end of the 72 hour acclimation period the rats generally show no interest in the clay pellets.

    [0299] At the end of 72 hours the rats are placed in clean cages and the food cups weighed. Rats that are still consuming clay regularly are removed from the study. Immediately prior to the dark cycle (the time when the animals are active and should be eating) the animals are split into treatment groups and dosed orally with a dose of the compound/salt of the invention (different doses for different treatment groups) or with vehicle alone, at a dose volume of 2 ml/kg. In this oral dosing, the compound/salt is in the form of a suspension in 0.5% methylcellulose (obtainable Sigma-Aldrich, St. Louis, MO, USA) in water. The food and clay cups and cage debris are weighed the following day and the total clay and food consumed that night by each individual animal is calculated.

    [0300] A dose response is calculated by first converting the data into quantal response, where animals are either positive or negative for the pica response. A rat is "pica positive" if it consumes greater than or equal to 0.3 grams of clay over the mean of is usually calculated using logistic regression performed by the Statistica software statistical package. A Pica Response ED50 value in mg per kg of body weight can then be calculated.

    [0301] The Pica Response ED50 value can be compared to the neutrophilia-inhibition ED50 values for the same compound administered orally to the rat (measurable by In Vivo Assay 1 above), so that a Therapeutic Index (TI) in rats can be calculated thus:



    [0302] In general, the Therapeutic Index (TI) calculated this way is often substantially different to, for example can often be substantially higher than, the TI (D20/D50 calculated in the ferret (see In vivo Assay 4 below).

    [0303] Results: Using the above procedure, and according to current measurements, the compounds of Examples 14, 17, 23, 35 and 38 exhibited a Pica Response ED50 in the range of about 2 mg/kg to greater than about 50 mg/kg, subject to testing inaccuracies. Taking the specific Pica Response ED50 values for these compounds together with the specific rat neutrophilia-inhibition ED50 values measured in In Vivo Assay 1 for Examples 14, 17, 23, 35 and 38, the Rat Therapeutic Index (TI) for orally-administered Examples 14, 17, 23, 35 and 38 was calculated using the above equation as being in the range of from about 12 to about 470, according to current measurements, subject to testing inaccuracies.

    Literature:



    [0304] Beavo JA, Contini, M., Heaslip, R.J. Multiple cyclic nucleotide phosphodiesterases. Mol Pharmacol. 1994; 46:399-405.

    [0305] Spond J, Chapman R, Fine J, Jones H, Kreutner W, Kung TT, Minnicozzi M. Comparison of PDE 4 inhibitors, Rolipram and SB 207499 (Ariflo™), in a rat model of pulmonary neutrophilia. Pulmonary Pharmacology and Therapeudtics. 2001; 14:157-164.

    [0306] Takeda N, Hasegawa S, Morita M, and Matsunaga T. Pica in rats is analogous to emesis: an animal model in emesis research. Pharmacology, Biochemistry and Behavior. 1991; 45:817-821.

    [0307] Takeda N, Hasegawa S, Morita M, Horii A, Uno A, Yamatodani A and Matsunaga T. Neuropharmacological mechanisms of emesis. I. Effects of antiemetic drugs on motion- and apomorphine-induced pica in rats. Meth Find Exp Clin Pharmacol. 1995; 17(9) 589-596.

    [0308] Takeda N, Hasegawa S, Morita M, Horii A, Uno A, Yamatodani A and Matsunaga T. Neuropharmacological mechanisms of emesis. II . Effects of antiemetic drugs on cisplatin-induced pica in rats. Meth Find Exp Clin Pharmacol. 1995; 17(9) 647-652.

    In Vivo Assay 3. LPS induced pulmonary neutrophilia in rats: effect of intratracheally administered PDE4 inhibitors



    [0309] This assay is an animal model of inflammation in the lung - specifically neutrophilia induced by lipopolysaccharide (LPS) - and allows the study of putative inhibition of such neutrophilia (anti-inflammatory effect) by intratracheally (i.t.) administered PDE4 inhibitors. The PDE4 inhibitors are preferably in dry powder or wet suspension form. I.t. administration is one model of inhaled administration, allowing topical delivery to the lung.

    [0310] Animals: Male CD (Sprague Dawley Derived) rats supplied by Charles River, Raleigh, NC, USA are housed in groups of 5 rats per cage, acclimatised after delivery for at least 7 days with bedding/nesting material regularly changed, fed on SDS diet R1 pelleted food given ad lib, and supplied with daily-changed pasteurised animal grade drinking water.

    [0311] Device for dry powder administration: Disposable 3-way tap between dosing needle and syringe. A 3-way sterile tap (Vycon Ref 876.00) is weighed, the drug blend or inhalation grade lactose (vehicle control) is then added to the tap, the tap closed to prevent loss of drug, and the tap is re-weighed to determine the weight of drug in the tap. After dosing, the tap is weighed again to determine the weight of drug that had left the tap. The needle, a Sigma Z21934-7 syringe needle 19-gauge 152 mm (6 inches) long with luer hub, is cut by engineering to approximately 132 mm (5.2 inches), a blunt end is made to prevent them damaging the rat's trachea, and the needle is weighed prior to and after drug delivery to confirm that no drug is retained in the needles after dosing.

    [0312] Device for wet suspension administration: This is the similar to the above but a blunt dosing needle, whose forward end is slightly angled to the needle axis, is used, with a flexible plastic portex canula inserted into the needle.

    [0313] Drugs and Materials: Lipopolysaccharide (LPS) (Serotype:0127:B8) (L3129 Lot 61K4075) was dissolved in phosphate-buffered saline (PBS). PDE4 inhibitors are used in size-reduced (e.g. micronised) form, for example according to the Micronisation Example given above. For dry powder administration of the drug, the Dry Powder Formulation Example given above, comprising drug and inhalation-grade lactose, can be used. The inhalation-grade lactose usually used (Lot E98L4675 Batch 845120) has 10% fines (10% of material under 15um particle size measured by Malvern particle size).

    [0314] Wet suspensions of the drug can be prepared by added the required volume of vehicle to the drug, the vehicle being used being a mixture of saline/tween (0.2% tween 80). The wet suspension was sonicated for 10 minutes prior to use.

    [0315] Preparation, and dosing with PDE 4 inhibitor: Rats are anaesthetised by placing the animals in a sealed Perspex chamber and exposing them to a gaseous mixture of isoflourane (4.5 %), nitrous oxide (3 litres.minute-1) and oxygen (1 litre.minute-1). Once anaesthetised, the animals are placed onto a stainless steel i.t. dosing support table. They are positioned on their back at approximately a 35° angle. A light is angled against the outside of the throat to highlight the trachea. The mouth is opened and the opening of the upper airway visualised. The procedure varies for wet suspension and dry powder administration of PDE4 inhibitors as follows:

    Dosing with a Wet suspension: A portex cannula is introduced via a blunt metal dosing needle that had been carefully inserted into the rat trachea. The animals are intratracheally dosed with vehicle or PDE4 inhibitor via the dosing needle with a new internal canula used for each different drug group. The formulation is slowly (10 seconds) dosed into the trachea using a syringe attached to the dosing needle.

    Dosing with a Dry Powder: The three-way tap device and needle are inserted into the rat trachea up to a pre-determined point established to be located approximately 1 cm above the primary bifurcation. Another operator holds the needle at the specified position whilst 2x 4ml of air is delivered through the three-way tap by depressing the syringes (ideally coinciding with the animal inspiring), aiming to expel the entire drug quantity from the tap. After dosing, the needle and tap are removed from the airway and the tap is closed off to prevent any retained drug leaving the tap.

    After dosing with either wet suspension or dry powder, the animals are then removed from the table and observed constantly until they have recovered from the effects of anaesthesia. The animals are returned to the holding cages and given free access to food and water; they are observed and any unusual behavioural changes noted.

    [0316] Exposure to LPS: About 2 hours after i.t. dosing with vehicle control or the PDE4 inhibitor, the rats are placed into sealed Perspex containers and exposed to an aerosol of LPS (nebuliser concentration 150 µg.ml-1) for 15 minutes. Aerosols of LPS are generated by a nebuliser (DeVilbiss, USA) and this is directed into the Perspex exposure chamber. Following the 15-minute LPS-exposure period, the animals are returned to the holding cages and allowed free access to both food and water.

    [0317] [In an alternative embodiment, the rats can exposed to LPS less than 2 hours after i.t. dosing. In another alternative embodiment, the rats can exposed to LPS more than 2 hours (e.g. ca. 4 or ca. 6 hours) after i.t. dosing by vehicle or PDE4 inhibitor, to test whether or not the PDE4 inhibitor has a long duration of action (which is not essential).]

    [0318] Bronchoalveolar lavage: 4 hours after LPS exposure the animals are killed by overdose of sodium pentobarbitone (i.p.). The trachea is cannulated with polypropylene tubing and the lungs lavaged (washed out) with 3 x 5 mls of heparinised (25 units.ml-1) phosphate buffered saline (PBS).

    [0319] Neutrophil cell counts: The Bronchoalveolar lavage (BAL) samples are centrifuged at 1300 rpm for 7 minutes. The supernatant is removed and the resulting cell pellet resuspended in 1 ml PBS. A cell slide of the resuspension fluid is prepared by placing 100µl of resuspended BAL fluid into cytospin holders and then spun at 5000 rpm for 5 minutes. The slides are allowed to air dry and then stained with Leishmans stain (20 minutes) to allow differential cell counting. The total cells are also counted from the resuspension. From these two counts, the total numbers of neutrophils in the BAL are determined. For a measure of PDE4-inhibitor-induced inhibition of neutrophilia, a comparison of the neutrophil count in rats treated with vehicle and rats treated with PDE4 inhibitors is conducted.

    [0320] By varying the dose of the PDE4 inhibitor used in the dosing step (e.g. 0.2 or 0.1 mg of PDE4 inhibitor per kg of body weight, down to e.g. 0.01 mg/kg), a dose-response curve can be generated.

    In vivo Assay 4. Evaluation of Therapeutic Index of Orally-administered PDE 4 inhibitiors in the consicous ferret


    1.1 Materials



    [0321] The following materials are used for these studies:

    PDE4 inhibitors are prepared for oral (p.o.) administration by dissolving in a fixed volume (1 ml) of acetone and then adding cremophor to 20% of the final volume.

    Acetone is evaporated by directing a flow of nitrogen gas onto the solution. Once the acetone is removed, the solution is made up to final volume with distilled water.

    LPS is dissolved in phosphate buffered saline.


    1.2 Animals



    [0322] Male ferrets (Mustela Pulorius Furo, weighing 1 - 2 kg) are transported and allowed to acclimatise for not less than 7 days. The diet comprises SDS diet C pelleted food given ad lib with Whiskers cat food given 3 times per week. The animals are supplied with pasteurised animal grade drinking water changed daily.

    1.3 Experimental Protocol(s)


    1.3.1 Dosing with PDE4 inhibitors



    [0323] PDE4 inhibitors are administered orally (p.o.), using a dose volume of 1ml/kg.

    [0324] Ferrets are fasted overnight but allowed free access to water. The animals are orally dosed with vehicle or PDE 4 inhibitor using a 15cm dosing needle that is passed down the back of the throat into the oesophagus. After dosing, the animals are returned to holding cages fitted with perspex doors to allow observation, and given free access to water. The animals are constantly observed and any emetic episodes (retching and vomiting) or behavioural changes are recorded. The animals are allowed access to food 60 - 90 minutes after p.o. dosing.

    1.3.2 Exposure to LPS



    [0325] Thirty minutes after oral dosing with compound or vehicle control, the ferrets are placed into sealed perspex containers and exposed to an aerosol of LPS (30 µg/ml) for 10 minutes. Aerosols of LPS are generated by a nebuliser (DeVilbiss, USA) and this is directed into the perspex exposure chamber. Following a 10-minute exposure period, the animals are returned to the holding cages and allowed free access to water, and at a later stage, food. General observation of the animals continues for a period of at least 2.5 hours post oral dosing. All emetic episodes and behavioural changes are recorded.

    1.3.3 Bronchoalveolar lavage and cell counts



    [0326] Six hours after LPS exposure the animals are killed by overdose of sodium pentobarbitone administered intraperitoneally. The trachea is then cannulated with polypropylene tubing and the lungs lavaged twice with 20 ml heparinised (10 units/ml) phosphate buffered saline (PBS). The bronchoalveolar lavage (BAL) samples are centrifuged at 1300 rpm for 7 minutes. The supernatant is removed and the resulting cell pellet re-suspended in 1 ml PBS. A cell smear of re-suspended fluid is prepared and stained with Leishmans stain to allow differential cell counting. A total cell count is made using the remaining re-suspended sample. From this, the total number of neutrophils in the BAL sample is determined.

    1.3.4 Pharmacodynamic readouts



    [0327] The following parameters are recorded:
    1. a) % inhibition of LPS-induced pulmonary neutrophilia to determine the dose of PDE4 inhibitor which gives 50% inhibition (D50).
    2. b) Emetic episodes - the number of vomits and retches are counted to determine the dose of PDE4 inhibitor that gives a 20% incidence of emesis (D20).
    3. c) A therapeutic index (TI), using this assay, is then calculated for each PDE4 inhibitor using the following equation:



    [0328] It is noted that the Ferret Therapeutic index (TI) (D20/D50) calculated using this in vivo Assay 4 is often substantially different to, and for example is often substantially lower than, the Rat TI (50/50) calculated using the rat oral inflammation and pica feeding Assays 1+2.

    [0329] The calculation of TI using the known PDE4 inhibitor roflumilast in this Assay 4 is: D20 for emesis = 0.46 mg/kg p.o., D50 for ferret neutroplilia = 0.42 mg/kg p.o., Ferret TI = 1.1.

    EXAMPLES



    [0330] The various aspects of the invention will now be described by reference to the following examples. These examples are merely illustrative and are not to be construed as a limitation of the scope of the present invention. In this section, "Intermediates" represent syntheses of intermediate compounds intended for use in the synthesis of the "Examples".

    Abbreviations used herein:



    [0331] 
    BEMP
    2-t-butylimino-2-diethylamino-1,3-dimethylperhydro-1,3,2-diazaphosphazine
    CDI
    1,1'-carbonyldiimidazole
    DBU
    1,8-Diazabicyclo[5.4.0]undec-7-ene
    DCM
    dichloromethane
    DMF
    dimethyl formamide
    DMSO
    dimethyl sulfoxide
    EtOAc
    ethyl acetate
    Et2O
    diethyl ether
    EDC
    1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride
    h
    hours
    HOBT
    hydroxybenzotriazole = 1-hydroxybenzotriazole
    HATU
    O-(7-Azabenzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate
    HBTU
    O-(Benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium hexafluorophosphate
    HPLC
    High performance liquid chromatography
    LCMS
    liquid chromatography / mass spectroscopy
    MeCN
    acetonitrile
    MeOH
    methanol
    NMR
    nuclear magnetic resonance (in which: s = singlet, d = doublet, t = triplet, q = quartet, dd = doublet of doublets, m = multiplet, n H means that n is the number of protons)
    DIPEA
    N,N-diisopropylethylamine (iPr2NEt)
    SPE
    solid phase extraction
    TBTU
    O-(Benzotriazol-1-yl)-N,N,N',N'-tetramethyluronium tetrafluoroborate
    THF
    Tetrahydrofuran
    TRET
    retention time (from LCMS)
    TLC
    thin layer chromatography
    Lawesson's
    reagent 2,4-bis(4-methoxyphenyl)-1,3-dithia-2,4-diphosphetane-2,4-disulphide
    Burgess Reagent
    (Methoxycarbonylsulphamoyl)triethylammonium hydroxide
    Room temperature
    = ambient temperature: this is usually in the range of about 15 to about 25 °C or about 20 to about 25 °C.

    Machine Methods used herein:


    LCMS (liquid chromatography / mass spectroscopy)



    [0332] Waters ZQ mass spectrometer operating in positive ion electrospray mode, mass range 100-1000 amu.
    UV wavelength : 215-330nM
    Column : 3.3cm x 4.6mm ID, 3µm ABZ+PLUS
    Flow Rate: 3ml/min
    Injection Volume: 5µl
    Solvent A : 95% acetonitrile + 0.05% formic acid
    Solvent B : 0.1 % formic acid + 10mMolar ammonium acetate
    Gradient: 0% A/0.7min, 0-100% A/3.5min, 100% A/1.1min, 100-0% A/0.2min

    Mass directed autoprep HPLC



    [0333] The prep column used was a Supelcosil ABZplus (10cm x 2.12cm) (usually 10cm x 2.12cm x 5 µm).
    UV wavelength: 200-320nM
    Flow : 20ml/min
    Injection Volume: 1ml; or more preferably 0.5 ml
    Solvent A : 0.1% formic acid (or 0.1% trifluoroacetic acid)
    Solvent B : 95% acetonitrile + 5% of (formic acid or trifluoroacetic acid); or more usually 99.95% acetonitrile + 0.05% of (formic acid or trifluoroacetic acid)
    Gradient: 100% A/1min, 100-80% A/9min, 80-1% A/3.5min, 1% A/1.4min, 1-100%A/0.1min

    Microwave



    [0334] The CEM Discover Focused Microwave Synthesis system was used.

    Intermediates and Examples



    [0335] All reagents not detailed in the text below are commercially available from established suppliers such as Sigma-Aldrich. The addresses of the suppliers for some of the starting materials mentioned in the Intermediates and Examples below or the Assays above are as follows:
    • ABCR GmbH & CO. KG, P.O. Box 21 01 35, 76151 Karlsruhe, Germany
    • Aceto Color Intermediates (catalogue name), Aceto Corporation, One Hollow Lane, Lake Success, NY, 11042-1215, USA
    • Acros Organics, A Division of Fisher Scientific Company, 500 American Road, Morris Plains, NJ 07950, USA
    • Apin Chemicals Ltd., 82 C Milton Park, Abingdon, Oxon OX14 4RY, United Kingdom
    • Apollo Scientific Ltd., Unit 1A, Bingswood Industrial Estate, Whaley Bridge, Derbyshire SK23 7LY, United Kingdom
    • Aldrich (catalogue name), Sigma-Aldrich Company Ltd., Dorset, United Kingdom, telephone: +44 1202 733114; Fax: +44 1202 715460; ukcustsv@eurnotes.sial.com; or
    • Aldrich (catalogue name), Sigma-Aldrich Corp., P.O. Box 14508, St. Louis, MO 63178-9916, USA; telephone: 314-771-5765; fax: 314-771-5757; custserv@sial.com; or
    • Aldrich (catalogue name), Sigma-Aldrich Chemie Gmbh, Munich, Germany; telephone: +49 89 6513 0; Fax: +49 89 6513 1169; deorders@eurnotes.sial.com.
    • Alfa Aesar, A Johnson Matthey Company, 30 Bond Street, Ward Hill, MA 01835-8099, USA
    • Amersham Biosciences UK Ltd, Pollards Wood, Chalfont St Giles, Buckinghamshire HP8 4SP, United Kingdom
    • Array Biopharma Inc., 1885 33rd Street, Boulder, CO 80301, USA
    • AstaTech, Inc., 8301 Torresdale Ave., 19C, Philadelphia, PA 19136, USA
    • Austin Chemical Company, Inc., 1565 Barclay Blvd., Buffalo Grove, IL 60089, USA
    • Avocado Research, Shore Road, Port of Heysham Industrial Park, Heysham Lancashire LA3 2XY, United Kingdom
    • Bayer AG, Business Group Basic and Fine Chemicals, D-51368 Leverkusen, Germany
    • Berk Univar plc, Berk House, P.O.Box 56, Basing View, Basingstoke, Hants RG21 2E6, United Kingdom
    • Butt Park Ltd., Braysdown Works, Peasedown St. John, Bath BA2 8LL, United Kingdom
    • Chemical Building Blocks (catalogue name), Ambinter, 46 quai Louis Bleriot, Paris, F-75016, France
    • ChemBridge Europe, 4 Clark's Hill Rise, Hampton Wood, Evesham, Worcestershire WR11 6FW, United Kingdom
    • ChemService Inc., P.O.Box 3108, West Chester, PA 19381, USA
    • Combi-Blocks Inc., 7949 Silverton Avenue, Suite 915, San Diego, CA 92126, USA
    • Dynamit Nobel GmbH, Germany; also available from: Saville Whittle Ltd (UK agents of Dynamit Nobel), Vickers Street, Manchester M40 8EF, United Kingdom
    • E. Merck, Germany; or E. Merck (Merck Ltd), Hunter Boulevard, Magna Park, Lutterworth, Leicestershire LE17 4XN, United Kingdom
    • Esprit Chemical Company, Esprit Plaza, 7680 Matoaka Road, Sarasota, FL 34243, USA
    • Exploratory Library (catalogue name), Ambinter, 46 quai Louis Bleriot, Paris, F-75016, France
    • Fluka Chemie AG, Industriestrasse 25, P.O. Box 260, CH-9471 Buchs, Switzerland
    • Fluorochem Ltd., Wesley Street, Old Glossop, Derbyshire SK13 7RY, United Kingdom
    • ICN Biomedicals, Inc., 3300 Hyland Avenue, Costa Mesa, CA 92626, USA
    • Interchim Intermediates (catalogue name), Interchim, 213 Avenue Kennedy, BP 1140, Montlucon, Cedex, 03103, France
    • Key Organics Ltd., 3, Highfield Indusrial Estate, Camelford, Cornwall PL32 9QZ, United Kingdom
    • Lancaster Synthesis Ltd., Newgate, White Lund, Morecambe, Lancashire LA3 3DY, United Kingdom
    • Manchester Organics Ltd., Unit 2, Ashville Industrial Estate, Sutton Weaver, Runcorn, Cheshire WA7 3PF, United Kingdom
    • Matrix Scientific, P.O. Box 25067, Columbia, SC 29224-5067, USA
    • Maybridge Chemical Company Ltd., Trevillett, Tintagel, Cornwall PL34 0HW, United Kingdom
    • Maybridge Reactive Intermediates (catalogue name), Maybridge Chemical Company Ltd., Trevillett, Tintagel, Cornwall PL34 0HW, United Kingdom
    • MicroChemistry Building Blocks (catalogue name), MicroChemistry-RadaPharma, Shosse Entusiastov 56, Moscow, 111123, Russia
    • Miteni S.p.A., Via Mecenate 90, Milano, 20138, Italy
    • Molecular Devices Corporation, Sunnydale, CA, USA
    • N.D. Zelinsky Institute, Organic Chemistry, Leninsky prospect 47, 117913 Moscow B-334, Russia
    • Optimer Building Block (catalogue name), Array BioPharma, 3200 Walnut Street, Boulder, CO 80301, USA
    • Peakdale Molecular Ltd., Peakdale Science Park, Sheffield Road, Chapel-en-le-Frith, High Peak SK23 0PG, United Kingdom
    • Pfaltz & Bauer, Inc., 172 East Aurora Street, Waterbury, CT 06708, USA
    • Rare Chemicals (catalogue name), Rare Chemicals GmbH, Schulstrasse 6, 24214 Gettorf, Germany
    • SALOR (catalogue name) (Sigma Aldrich Library of Rare Chemicals), Aldrich Chemical Company Inc, 1001 West Saint Paul Avenue, Milwaukee, WI 53233, USA
    • Sigma (catalogue name), Sigma-Aldrich Corp., P.O. Box 14508, St. Louis, MO 63178-9916, USA; see "Aldrich" above for other non-US addresses and other contact details
    • SIGMA-RBI, One Strathmore Road, Natick, MA 01760-1312, USA
    • Synchem OHG Heinrich-Plett-Strasse 40, Kassel, D-34132, Germany
    • Syngene International Pvt Ltd, Hebbagodi, Hosur Road, Bangalore, India.
    • TCI America, 9211 North Harborgate Street, Portland, OR 97203, USA
    • TimTec Building Blocks A, TimTec, Inc., P O Box 8941, Newark, DE 19714-8941, USA
    • Trans World Chemicals, Inc., 14674 Southlawn Lane, Rockville, MD 20850, USA
    • Ubichem PLC, Mayflower Close, Chandlers Ford Industrial Estate, Eastleigh, Hampshire S053 4AR, United Kingdom
    • Ultrafine (UFC Ltd.), Synergy House, Guildhall Close, Manchester Science Park, Manchester M15 6SY, United Kingdom
    Table of Intermediates
    Intermediate NumberName
    1 Ethyl 4-chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate
    2 Ethyl 4-(cyclopentylamino)-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate
    3 4-(Cyclopentylamino)-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid
    4 N'-Acetyl-4-(Cyclopentylamino)-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    5 4-(Cyclopentylamino)-1-ethyl-N'-[(methylsulfonyl)acetyl]-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    6 Ethyl 4-(4-fluorophenylamino)-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate
    7 4-(Cyclopentylamino)-1-ethyl-N-[methyl]-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    8 Methanesulfonyl acetic acid hydrazide
    9 Acetamidoxime
    10 4-(Cyclopentylamino)-1-ethyl-N'-isobutyryl-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    11 4-Chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid
    12 4-Chloro-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridine
    13 4-Chloro-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridine
    14 5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)4-chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridine
    15 4-Chloro-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridine
    16 Ethyl 1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylate
    17 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid
    18 Tert-butyl 2-{[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}hydrazinecarboxylate
    19 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide dihydrochloride
    20 N'-(Cyclopropylcarbonyl)-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    21 Tetrahydro-2H-pyran-4-amine = 4-Aminotetrahydropyran
    21A Tetrahydro-2H-pyran-4-amine hydrochloride = 4-aminotetrahydropyran hydrochloride
    22 N'-Hydroxy-2-methoxyethanimidamide
    23 2-(Dimethylamino)-N'-hydroxyethanimidamide
    24 N'-Hydroxy-2-morpholin-4-ylethanimidamide
    25 1-Acetyl-4-aminopiperidine hydrochloride
    26 3-Methyloxetane-3-carboxylic acid
    27 (4-Methylpiperazin-1-yl)acetic acid
    28 (Isopropylamino)(oxo)acetic acid
    29 I-Methyl-5-oxopyrrolidine-3-carboxylic acid
    30 Tetrahydro-2H-pyran-4-carboxylic acid
    31 Morpholin-4-ylacetic acid
    32 Tert-butoxyacetic acid
    33 Methyl (2S)-2-({[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}amino)-3-hydroxypropanoate
    34 1-Ethyl-N-(2-hydroxy-1-methylethyl)-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
    35 Ethyl 4-ethoxy-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate
    36 Ethyl 4-emoxy-1H-pyramolo[3,4-b]pyridine-5-carboxylate
    37 Ethyl 4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylate
    38 Ethyl 1-n-propyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylate
    39 1-n-Propyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid
    40 N'-Acetyl 1-n-propyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    41 Ethyl 4-[(1-acetyl-4-piperidinyl)amino]-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate
    42 1-Ethyl-N-[(1R)-2-hydroxy-1-phenylethyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
    43 1-Ethyl-N-[(1S)-2-hydroxy-1-phenylethyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
    44 1-Ethyl-N-[(1S)-2-hydroxy-1-(phenylmethyl)ethyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
    45 1-Ethyl-N-[(1R)-2-hydroxy-1-(phenylmethyl)ethyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
    46 1-Ethyl-N-[(1S,2R)-2-hydroxy-1-phenylpropyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
    47 1-Ethyl-N-[(2R)-2-hydroxy-2-phenylethyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
    48 1-Ethyl-N-[(2S)-2-hydroxy-2-phenylethyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4,b]pyridine-5-carboxamide
    49 1-Ethyl-N-(2-hydroxy-1,1-dimethylethyl)-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
    50 N-methyltetrahydro-2H-pyran-4-amine (Reference Intermediate)
    51 Ethyl 1-ethyl-4-[methyl(tetrahydro-2H-pyran-4-yl)amino]-1H-pyrazolo[3,4-b]pyridine-5-carboxylate (Reference Intermediate)
    52 1-Ethyl-4-[methyl(tetrahydro-2H-pyran-4-yl)amino]-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid (Reference Intermediate)
    53 N'-Acetyl-1-ethyl-4-[methyl(tetrahydro-2H-pyran-4-yl)amino]-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide (Reference Intermediate)
    54 trans-4-Aminocyclohexanol
    55 Tetrahydro-2H-pyran-3-amine hydrochloride
    56 4-Aminocyclohexanone hydrochloride
    57 N-Propyltetrahydro-2H-pyran-4-amine (Reference Intermediate)
    58 Ethyl 4-chloro-1-ethyl-6-methyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate
    59 Ethyl 1-ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylate
    60 1-Ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid
    61 N'-(2,2-Dimethylpropanoyl)-1-ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    62 1,1-Dimethylethyl 2-{[1-ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}hydrazinecarboxylate
    63 1-Ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide hydrochloride
    64 1-Ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-N'-(tetrahydro-2H-pyran-4-ylcarbonyl)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    65 N'-(Cyclobutylcarbonyl)-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    66 1-Ethyl-N'-[(5-oxo-2-pyrrolidinyl)carbonyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide (non-preferred name)
    67 N-[2-(2-{[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}hydrazino)-2-oxoethyl]acetamide (non-preferred name)
    68 1-Ethyl-N'-[(1-methyl-2-piperidinyl)carbonyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    69 1-Ethyl-N'-[(4-methyl-1,2,5-oxadiazol-3-yl)acetyl]-4-(tetrahydro-2H pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    70 1-Ethyl-N'-[(3-oxocyclopentyl)carbonyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    71 1-Ethyl-N'-(tetahydro-3-furanylcarbonyl)-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    72 1-ethyl-N'-[(2-oxo-1,3-thiazolidin-4-yl)carbonyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    73 N'-[(2,2-Dimethylcyclopropyl)carbonyl]-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    74 N-[2-(2-{[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}hydrazino)-2-oxoethyl]-N-methylacetamide (non-preferred name) -
    75 1-Ethyl-N'-(tetrahydro-2H-pyran-4-ylacetyl)-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    76 1-Ethyl-N'-[(1-methylcyclobutyl)carbonyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridke-5-carbohydrazide
    77 1-Ethyl-N'-[(3-methyl-5-isoxazolyl)carbonyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    78 1-Ethyl-N'-[(1-methyl-1H-pyrazol-5-yl)carbonyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    79 N'-[(1-Acetyl-4-piperidinyl)carbonyl]-1-ethyl-4-(tetrthydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    80 (1E/Z)-N-hydroxy-2-(4-methyl-1-piperazinyl)ethanimidamide
    81 4-Fluoro-N-hydroxybenzenecarboximidamide
    82 (1E/Z)-N-hydroxy-3-oxo-3-(1-pyrrolidinyl)propanimidamide
    83 {5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}acetic acid
    84 1,1-Dimethylethyl {5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}acetate
    85 1,1-Dimethylethyl (3E/Z)-3-(hydroxyamino)-3-iminopropanoate
    86 N"-{[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}-N,N,N',N'-tetramethylcarbonohydrazonic diamide
    87 Ethyl (2-methyl-1,3-thiazol-4-yl) acetate
    88 2-Methyl-1,3-thiazol-4-yl acetic acid
    89 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-N'-(1H-1,2,3-triazol-1-ylacetyl)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    90 N'-[(2,4-Dimethyl-1,3-thiazol-5-yl)acetyl]-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    91 1-Ethyl-N'-(2-furanylacetyl)-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    92 1-Ethyl-N'-(3-isoxazolylacetyl)-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    93 1-Ethyl-N'-{[4-(methyloxy)phenyl]acetyl}-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    94 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-N'-(1H-tetrazol-1-ylacetyl)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    95 1-Ethyl-N'-(5-isothiazolylacetyl)-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    96 1-Ethyl-N'-[(3-methyl-5-isoxazolyl)acetyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    97 N'-{[4-(Diniethylamino)phenyl]acetyl}-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    98 1-Ethyl-N'-[(2-methyl-1,3-thiazol-4-yl)acetyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    99 2-{1-[2-(2-{[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}hydrazino)-2-oxoethyl]cyclopentyl}-N-methylacetamide
    100 N-[2-(2-{[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}hydrazino)-2-oxoethyl]cyclopropanecarboxamide (non-preferred name)
    101 1-Ethyl-N'-[(5-methyl-3-isoxazolyl)carbonyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    102 1-Ethyl-N'-[(5-methyl-3-isoxazolyl)acetyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    103 1-Ethyl-N'-[3-(4-methyl-1,3-thiazol-5-yl)propanoyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    104 1-Ethyl-N'-[(6-oxo-2-piperidinyl)carbonyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    105 1-Ethyl-N'-[(3-methyl-1H-1,2,4-triazol-5-yl)acetyl]-4-(tetrthydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    106 N'-[(3,5-Dimethyl-4-isoxazolyl)acetyl]-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    107 N-[2-(2-{[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}hydrazino)-1-methyl-2-oxoethyl]acetamide (non-preferred name)
    108 N'-[(1-Acetyl-4-piperidinyl)acetyl]-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    109 1-Ethyl-N'-[(4-methylphenyl)acetyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    110 1-Ethyl-N'-[(4-methylphenyl)carbonyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    111 N'-[(3,4-Dimethylphenyl)carbonyl]-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    112 N'-[(2,4-Dimethylphenyl)carbonyl]-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    113 N'-[(2,4-Dimethylphenyl)acetyl]-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    114 N'-[(4-Bromophenyl)acetyl]-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide
    115 4-Fluoro-N-hydroxybenzenecarboximidamide
    116 1,1-Dimethylethyl [(2Z)-2-(hydroxyamino)-2-iminoethyl]carbamate
    117 1,1-Dimethylethyl ({5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)carbamate
    118 5-[3-(Aminomethyl)-1,2,4-oxadiazol-5-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    119 4-Chloro-N-({5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)butanamide
    120 5-Chloro-N-({5-[1-ethyl-4-(tetahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)pentanamide
    121 (1E/Z)-N-hydroxy-2-(4-morpholinyl)propanimidamide
    122 (1E/Z)-2-cyclohexyl-N-hydroxyethanimidamide
    123 1,1-Dimethylethyl 4-[(2Z)-2-(hydroxyamino)-2-iminoethyl]-1-piperidinecarboxylate
    124 1,1-Dimethylethyl 4-({5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-1-piperidinecarboxylate
    125 1-Ethyl-5-[3-(4-piperidinylmethyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine hydrochloride
    126 N-Hydroxy-1-(phenylsulfonyl)cyclopropanecarboximidamide
    127 (1E/Z)-N-Hydroxy-2-phenylethanimidamide
    128 (1E/Z)-N-Hydroxy-2-phenylpropanimidamide
    129 (1E/Z)-N-Hydroxy-2-[4-(methyloxy)phenyl]ethanimidamide
    130 (1E/Z)-N-Hydroxy-2-[3-(methyloxy)phenyl]ethanimidamide
    131 (1E/Z)-2-[4-(Dimethylamino)phenyl]-N-hydroxyethanimidamide
    132 (1E/Z)-2-[3-(Dimethylamino)phenyl]-N-hydroxyethanimidamide
    133 (1E/Z)-N-Hydroxy-2-(phenyloxy)ethanimidamide
    134 (1E/Z)-N-hydroxy-2-(5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyridin-3-yl)ethanimidamide
    135 (1E/Z)-N-Hydroxy-2-(4-phenyl-1-piperazinyl)ethanimidamide
    136 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide
    137 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbonitrile
    138 1-Ethyl-N-hydroxy-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboximidamide
    139 1-Ethyl-N-[4-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide

    Intermediate 1: Ethyl 4-chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0336] Prepared from commercially available 5-amino-1-ethyl pyrazole as described by G. Yu et. al. in J. Med Chem., 2001, 44, 1025-1027:


    Intermediate 2: Ethyl 4-(cyclopentylamino)-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0337] 



    [0338] Intermediate 1 (0.051g) and cyclopentyl amine (0.019g) were suspended in ethanol (2ml) and triethylamine (0.14ml) was added. The mixture was stirred under nitrogen and heated at 80°C for 16h. After cooling to room temperature, ethanol was removed by evaporation under a stream of nitrogen and the residue partitioned between DCM and water. The organic layer was loaded directly onto an SPE cartridge (silica, 5g) and eluted sequentially with; (i) DCM, (ii) DCM : Et2O (2:1), (iii) DCM : Et2O (1:1), (iv) Et2O, (v) EtOAc and (vi) MeOH. Fractions containing desired material were combined and concentrated in vacuo to afford Intermediate 2 (0.074g). LCMS showed MH+ = 303; TRET = 3.45min

    Intermediate 3: 4-(Cyclopentylamino)-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid



    [0339] 



    [0340] A solution of Intermediate 2 (2.2g) in ethanol: water (95:5, 16.85ml) was treated with sodium hydroxide (1.2g) and heated at 50°C for 16h. The mixture was concentrated in vacuo and the residue re-dissolved in water (0.85ml). The solution was acidified to pH4 using acetic acid and the resultant white precipitate was collected by filtration and dried under vacuum to afford Intermediate 3 (1.9g). LCMS showed MH+ = 275; TRET = 2.65min

    Intermediate 4: N'-Acetyl-4-(Cyclopentylamino)-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide



    [0341] 



    [0342] Intermediate 3 (0.066g), EDC (0.06g) and HOBT (0.035g) were suspended in DMF (2ml) and the mixture was stirred for 15 minutes. Acetic hydrazide (0.02g) was then added and the mixture stirred under nitrogen for 18h. Solvents were removed by concentration in vacuo and the residue partitioned between DCM and water. The layers were separated and the organic phase was washed with saturated aqueous sodium bicarbonate solution, then concentrated and applied to an SPE cartridge (aminopropyl, 1g) which was eluted with methanol. Concentration in vacuo afforded Intermediate 4 (0.043g). LCMS showed MH+ = 331; TRET = 2.38min.

    Intermediate 5: 4-(Cyclopentylamino)-1-ethyl-N'-[(methylsulfonyl)acetyl]-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide



    [0343] 



    [0344] Intermediate 3 (0.12g), EDC (0.12g) and HOBT (0.072g) were suspended in DMF (2ml) and stirred for 15 minutes. Intermediate 8 (0.082g) was then added and the mixture stirred under nitrogen for 18h. Reaction was incomplete so a further portion of Intermediate 8 was added (0.040g) and stirring continued for a further 66h. Solvents were removed in vacuo and the residue partitioned between DCM and water. The aqueous phase was further extracted with DCM and the combined organic layers applied to an SPE cartridge (silica, 5g) which was eluted sequentially with a gradient of Et2O: MeOH (1:0, 9:1, 8:2, 7:3 and 6:4). Fractions containing desired material were combined and concentrated in vacuo to afford Intermediate 5 (0.154g). LCMS showed MH+ = 409; TRET = 2.42min.

    Intermediate 6: Ethyl 4-(4-fluorophenylamino)-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0345] 



    [0346] Intermediate 1 (0.051g) and 4-fluoroaniline (0.024g) were suspended in ethanol (2ml) and triethylamine (0.14ml) was added. The mixture was stirred under nitrogen and heated at 80°C for 16h. After cooling to room temperature, ethanol was removed by evaporation under a stream of nitrogen and the residue partitioned between DCM and water. The organic layer was loaded directly onto an SPE cartridge (silica, 5g) and eluted sequentially with; (i) DCM, (ii) DCM : Et2O (2:1), (iii) DCM : Et2O (1:1), (iv) Et2O, (v) EtOAc, (vi) MeOH. Fractions containing desired material were combined and concentrated in vacuo to afford Intermediate 6 (0.077g). LCMS showed MH+ = 328; TRET = 3.36min.

    Intermediate 7: 4-(Cyclopentylamino)-1-ethyl-N-[methyl]-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide



    [0347] 

    Intermediate 3 (0.10g) was dissolved in DMF (2ml) and treated with HBTU (0.136g) and DIPEA (0.116g). A separate portion of Intermediate 3 (0.10g) was dissolved in DMF (2ml) and treated with EDC (0.096g) and HOBT (0.058g). The resultant suspensions were both stirred under nitrogen for 15min, then methyl hydrazine (0.017g) added to each and stirring continued under nitrogen for 18h. The mixtures were independently concentrated in vacuo and the residues partitioned between DCM and water. The organic layers were concentrated and each applied to an SPE cartridge (aminopropyl, 2g) which was eluted with methanol, followed by 10% ammonia in methanol. The two portions of Intermediate 7 thus afforded were combined (0.16g). LCMS showed MH+ = 303; TRET = 2.22min.

    Intermediate 8: Methanesulfonyl acetic acid hydrazide



    [0348] Prepared from commercially available ethyl methylsulphonyl acetate as described by D. E. Bays et. al. in EP 50407 :


    Intermediate 9: Acetamidoxime



    [0349] 



    [0350] Can be prepared from aqueous hydroxylamine and acetonitrile as described by J. J. Sahbari et. al. in WO 00/032565.

    Intermediate 10: 4-(Cyclopentylamino)-1-ethyl-N'-isobutyryl-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide



    [0351] 



    [0352] Intermediate 3 (0.060g), EDC (0.06g) and HOBT (0.035g) were suspended in DMF (2ml) and stirred under nitrogen for 15 minutes. Isobutyric acid hydrazide (0.027g) was then added and the mixture stirred under nitrogen for 18h. Solvents were removed in vacuo and the residue partitioned between DCM and water. The organic phase was washed with saturated aqueous sodium bicarbonate solution, then concentrated in vacuo and applied to an SPE cartridge (aminopropyl, 1g) which was eluted with methanol. Concentration in vacuo afforded Intermediate 10. LCMS showed MH+ = 359; TRET = 2.70min.

    Intermediate 11: 4-Chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid



    [0353] 



    [0354] A solution of Intermediate 1 (3.5g) in dioxane (28ml) was treated with potassium hydroxide (6.3g) as a solution in water (20ml). The mixture was stirred for 2h, then concentrated in vacuo, acidified to pH 3 with 2M aqueous hydrochloric acid and extracted with ethyl acetate. The layers were separated, the organic layer dried over sodium sulphate, then concentrated in vacuo to afford Intermediate 11 as a white solid (2.4g). LCMS showed MH+ = 226; TRET = 2.62min.

    Intermediate 12: 4-Chloro-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridine



    [0355] 



    [0356] Intermediate 11 (0.4g) was dissolved in thionyl chloride (3ml) and the mixture was heated at reflux (95°C) with stirring for 1h. After cooling to room temperature, excess thionyl chloride was removed by evaporation under reduced pressure and the resultant solid dissolved in anhydrous acetonitrile (2ml). This solution was added to a solution of acetic hydrazide (0.145g) and diisopropylethylamine (0.465ml) in anhydrous acetonitrile (2ml), and the mixture stirred for a further 2h. The mixture was concentrated in vacuo and the residue treated directly with phosphorus oxychloride (4ml). The resultant solution was stirred and heated at reflux (120°C) for 0.5h, then allowed to cool and purified by Biotage (silica, 40g), eluting with cyclohexane : EtOAc (1:1) to afford Intermediate 12 (0.32g). LCMS showed MH+ = 264; TRET = 2.55 min.

    Intermediate 13: 4-Chloro-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridine



    [0357] 



    [0358] Intermediate 11 (0.05g) was dissolved in thionyl chloride (1ml) and the mixture was heated at reflux (95°C) with stirring for 1h. After cooling to room temperature, excess thionyl chloride was removed by evaporation under reduced pressure and the resultant solid dissolved in anhydrous acetonitrile (0.5ml). This solution was added to a solution of isobutyric acid hydrazide (0.025g) and diisopropylethylamine (0.058ml) in anhydrous acetonitrile (1ml), and the mixture stirred for a further 1.5h. The mixture was concentrated in vacuo and the residue treated directly with phosphorus oxychloride (2ml). The resultant solution was stirred and heated at reflux (120°C) for 2h, then allowed to cool and concentrated in vacuo. The residue was applied to an SPE cartridge (silica, 5g) which was eluted sequentially with a gradient of EtOAc : cyclohexanI (i) 1:16, (ii) 1:8, (iii) 1:4, (iv) 1:2, (v) 1:1 and (vi) 1:0. Fractions containing desired material were combined and concentrated in vacuo to afford Intermediate 13 (0.049g). LCMS showed MH+ = 292; TRET = 2.96min.

    Intermediate 14: 5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-4-chloro-1-ethyl-1H-pyrazolo[3,4-b]pyridine



    [0359] 

    Intermediate 11 (0.40g) was dissolved in thionyl chloride (3ml) and the mixture was heated at reflux (95°C) with stirring for 1h. After cooling to room temperature, excess thionyl chloride was removed by evaporation under reduced pressure and the resultant solid dissolved in anhydrous acetonitrile (2ml). This solution was added to a solution of pivalic acid hydrazide (0.228g) and diisopropylethylamine (0.465ml) in anhydrous acetonitrile (2ml), and the mixture stirred for a further 1.5h. The mixture was concentrated in vacuo and the residue treated directly with phosphorus oxychloride (5ml). The resultant solution was stirred and heated at reflux (120°C) for 1.5h, then allowed to cool, concentrated in vacuo and purified by Biotage (silica, 40g), eluting with petroleum ether (40/60) : EtOAc (1:1) to afford Intermediate 14 (0.388g). LCMS showed MH+ = 306; TRET = 3.14 min.

    Intermediate 15: 4-Chloro-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridine



    [0360] 



    [0361] Intermediate 11 (0.68g) was dissolved in thionyl chloride (4ml) and the mixture was heated at reflux (95°C) with stirring for 1h. After cooling to room temperature, excess thionyl chloride was removed by evaporation under reduced pressure and the resultant solid dissolved in anhydrous acetonitrile (3ml). This solution was added dropwise over 5 minutes to a solution of Intermediate 8 (0.504g) and diisopropylethylamine (0.787ml) in anhydrous acetonitrile (12ml), and the mixture then stirred for a further 1h. The mixture was concentrated in vacuo and the residue treated directly with phosphorus oxychloride (8ml). The resultant solution was stirred and heated at reflux (120°C) for 2.5h, then allowed to cool, concentrated in vacuo and purified by Biotage (silica, 40g), eluting first with petroleum ether (40/60) : EtOAc (2:1), then with petroleum ether (40/60) : EtOAc (1:1). Fractions containing desired material were combined, concentrated in vacuo and the residue further purified by trituration with diethyl ether to afford Intermediate 15 (0.41g). LCMS showed MH+ = 342; TRET = 2.46 min.

    Intermediate 16: Ethyl 1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0362] 



    [0363] Intermediate 1 (0.20g) and triethylamine (0.55ml) were suspended in ethanol (8ml) and 4-aminotetrahydropyran (Intermediate 21, 0.088g) was added. The mixture was stirred under nitrogen, heated at 80°C for 16h, then concentrated in vacuo. The residue was partitioned between DCM and water. The layers were separated and the organic layer was loaded directly onto an SPE cartridge (silica, 5g) which was eluted sequentially witI; (i) DCM, (ii) DCM : Et2O (2:1), (iii) DCM : Et2O (1:1), (iv) Et2O and (v) EtOAc. Fractions containing desired material were combined and concentrated in vacuo to afford Intermediate 16 (0.21 g). LCMS showed MH+ = 319; TRET = 2.93min.

    Intermediate 17: 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid



    [0364] 



    [0365] A solution of Intermediate 16 (0.21 g) in ethanol : water (95:5, 10ml) was treated with sodium hydroxide (0.12g). The mixture was heated at 50°C for 8h, then concentrated in vacuo, dissolved in water and acidified to pH 4 with acetic acid. The resultant white solid was removed by filtration and dried under vacuum to afford Intermediate 17 as an off-white solid (0.16g). LCMS showed MH+ = 291; TRET = 2.11min.

    [0366] An alternative preparation of Intermediate 17 is as follows:
    A solution of Intermediate 16 (37.8g) in ethanol : water (4:1, 375ml) was treated with sodium hydroxide (18.9g). The mixture was heated at 50 °C for 5 hours, then concentrated in vacuo, dissolved in water and acidified to pH 2 with aqueous hydrochloric acid (2M). The resultant white solid was removed by filtration and dried under vacuum to afford Intermediate 17 as an off-white solid (29.65g). LCMS showed MH+ = 291; TRET = 2.17 min.

    Intermediate 18: Tert-butyl 2-{[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}hydrazinecarboxylate



    [0367] 

    A suspension of Intermediate 17 (1.48g), EDC (1.34g) and HOBT (0.83g) in DMF (20ml) was stirred at room temperature for 30min. t-Butyl carbazate (0.68g) was then added and stirring continued under nitrogen for a further 66h. The mixture was concentrated in vacuo and the residue divided into two portions for purification. Each portion was applied to an SPE cartridge (aminopropyl, 10g) which was eluted with methanol and the combined eluents were concentrated in vacuo. Further purification was carried out by Biotage (silica, 40g), eluting with cyclohexane : ethyl acetate (1:4). Fractions containing desired material were combined and concentrated in vacuo to afford Intermediate 18 (1.39g). LCMS showed MH+ = 405; TRET = 2.64min.

    Intermediate 19: 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide dihydrochloride



    [0368] 



    [0369] Intermediate 18 (1.39g) was treated with a 4M solution of hydrochloric acid in dioxane (8ml) and the mixture stirred under nitrogen for 1h. Concentration in vacuo afforded Intermediate 19 as a white solid (1.17g). LCMS showed MH+ = 305; TRET = 2.04min.

    Intermediate 20: N'-(Cyclopropylcarbonyl)-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide



    [0370] 



    [0371] A solution of Intermediate 19 (0.045g) in THF (2ml) was treated with DIPEA (0.045ml), then with cyclopropylcarbonyl chloride (0.013g) and stirred at room temperature for 16h. The mixture was concentrated in vacuo and the residue partitioned between dichloromethane and water. The layers were separated and the organic layer concentrated in vacuo, then applied to an SPE cartridge (aminopropyl, 1g). The column was eluted with methanol to afford Intermediate 20 as a white solid (0.02g). LCMS showed MH+ = 373; TRET = 2.15min.

    Intermediate 21: 4-Aminotetrahydropyran



    [0372] 



    [0373] Commercially available from Combi-Blocks Inc., 7949 Silverton Avenue, Suite 915, San Diego, CA 92126. CAS (Chemical Abstracts) Registry Number 38041-19-9.

    Intermediate 21A: Tetrahydro-2H-pyran-4-amine hydrochloride = 4-Aminotetrahydropyran hydrochloride



    [0374] 


    Step1: N,N-dibenzyltetrahydro-2H-pyran-4-amine



    [0375] Dibenzylamine (34.5g) and acetic acid (6.7ml) were added to a stirred solution of tetrahydro-4H-pyran-4-one (16.4g, commercially available from e.g. Aldrich) in dichloromethane (260ml) at 0 °C to 5 °C. After 2.5h at 0 °C to 5 °C, sodium triacetoxyborohydride (38.9g) was added portionwise, and the mixture was allowed to warm to room temperature. After stirring at room temperature overnight, the reaction mixture was washed successively with 2M-sodium hydroxide (200ml and 50ml), water (2 x 50ml) and brine (50ml), then dried and evaporated to give a yellow oil (45g). This oil was stirred with methanol (50ml) at 4 °C for 30min to give the product as a white solid (21.5g). LCMS showed MH+= 282; TRET = 1.98 min.

    Step 2: Tetrahydio-2H-pyran-4-amine hydrochloride



    [0376] N,N-dibenzyltetrahydro-2H-pyran-4-amine (20.5g) was dissolved in ethanol (210ml) and hydrogenated over 10% palladium on carbon catalyst (4g) at 100 psi for 72h at room temperature. The reaction mixture was filtered and the filtrate was adjusted to pH 1 with 2M-hydrogen chloride in diethyl ether. Evaporation of solvents gave a solid which was triturated with diethyl ether to give the product as a white solid (9.23g). 1H NMR (400MHz, d6-DMSO, δppm) 8.24 (br. s, 3H), 3.86 (dd, 12, 4Hz, 2H), 3.31 (dt, 2, 12Hz, 2H), 3.20 (m, 1H), 1.84 (m, 2H), 1.55 (dq, 4, 12Hz, 2H).

    Intermediate 22: N'-Hydroxy-2-methoxyethanimidamide



    [0377] 



    [0378] A solution ofmethoxyacetonitrile (12.26g) in ethanol (220ml) was treated with hydroxylamine hydrochloride (11.95g) followed by potassium carbonate (22.9g) and heated under reflux for 2 days. The mixture was concentrated in vacuo, then partitioned between ethylacetate and water. The organic layer was concentrated in vacuo to afford Intermediate 22 as a colourless liquid (7.6g). 1H NMR (CDCl3) 7.16 (3H, s), 7.67 (s, 2H), 9.32 (brs, 2H), 13.08 (1H, s).

    Intermediate 23: 2-(Dimethylamino)-N'-hydroxyethanimidamide



    [0379] 



    [0380] Can be prepared in an analogous manner to Intermediate 9, starting from dimethylamino acetonitrile.

    Intermediate 24: N'-Hydroxy-2-morpholin-4-ylethanimidamide



    [0381] 



    [0382] Can be prepared in an analogous manner to Intermediate 9, starting from morpholino acetonitrile (itself commercially available from TCI America, 9211 North Harborgate Street, Portland, OR 97203, USA).

    Intermediate 25: 1-Acetyl-4-aminopiperidine hydrochloride



    [0383] Prepared from commercially available N1-benzyl-4-aminopiperidine as described by Yamada et. al. In WO 00/42011:


    Intermediate 26: 3-Methyloxetane-3-carboxylic acid



    [0384] 



    [0385] Can be prepared by oxidation of 3-Methyl-3-oxetanemethanol (commercially available from e.g. Fluka, CAS (Chemical Abstracts) Registry Number 3143-02-0) according to the procedure described by H. Fiege et. al. in DE3618142.

    Intermediate 27: (4-Methylpiperazin-1-yl)acetic acid



    [0386] 



    [0387] Commercially available from ChemPacific USA Sales Marketing and Research Center, 6200 Freeport Centre, Baltimore, MD 21224, USA (CAS Registry Number 54699-92-2).

    Intermediate 28: (Isopropylamino)(oxo)acetic acid



    [0388] 



    [0389] Commercially available from Austin Chemical Company, Inc., 1565 Barclay Blvd., Buffalo Grove, IL 60089, USA. CAS (Chemical Abstracts) Registry Number 3338-22-5.

    Intermediate 29: 1-Methyl-5-oxopyrrolidine-3-carboxylic acid



    [0390] 



    [0391] Commercially available from MicroChemistry-RadaPharma, Shosse Entusiastov 56, Moscow 111123, Russia. CAS (Chemical Abstracts) Registry Number 42346-68-9.

    Intermediate 30: Tetrahydro-2H-pyran-4-carboxylic acid



    [0392] 



    [0393] Commercially available from Combi-Blocks Inc., 7949 Silverton Avenue, Suite 915, San Diego, CA 92126, USA. CAS (Chemical Abstracts) Registry Number 5337-03-1.

    Intermediate 31: Morpholin-4-ylacetic acid



    [0394] 



    [0395] Can be prepared from ethyl bromoacetate as described by Z. Dega-Szafran et. al. in J. Molecular Structure, 2001, 560, 261-273.

    Intermediate 32: Tert-butoxyacetic acid



    [0396] 



    [0397] A suspension of sodium t-butoxide (24.1g) in t-butanol (150ml) was cooled in a water bath and treated drop-wise with a solution of chloroacetic acid (11.4g) in t-butanol (30ml). The mixture was heated under reflux for 5h then concentrated in vacuo. The resultant white solid was dried in vacuo for 16h then water (100ml) was added and the mixture was filtered. The filtrate was treated with diethyl ether (150ml), then cooled in an ice bath, stirred and acidified to pH1 with 2N sulphuric acid. The layers were separated and the aqueous layer was further extracted with diethyl ether. The combined organic extracts were dried (MgSO4) and concentrated in vacuo to afford Intermediate 32 (11.1g). 1H NMR (400MHz, CDCl3, δppm) 1.27 (9H, s), 4.04 (2H, s).

    Intermediate 33: Methyl (2S)-2-({[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}amino)-3-hydroxypropanoate



    [0398] 





    [0399] Intermediate 17 (0.1g, 0.34mmol), EDC (0.066g, 0.34mmol) and HOBT (0.05g, 0.37mmol) were suspended in DMF (2ml) and stirred at room temperature under nitrogen for 15 mins. L-Serine methyl ester hydrochloride (0.054g, 0.34mmol) and triethylamine (0.036g, 0.36mmol) were added and the mixture stirred at room temperature under nitrogen for 18 hours. Solvents were removed in vacuo and the residue was partitioned between DCM and water. The organic layer was concentrated in vacuo and applied to an SPE cartridge (aminopropyl, 5g), which was eluted with methanol. Concentration in vacuo afforded an impure residue which was further purified by SPE cartridge (silica, 5g), eluting with ethyl acetate followed by 5% methanol/ethyl acetate. The desired fractions were concentrated in vacuo to afford Intermediate 33 (0.055g). LCMS showed MH+ = 393; TRET = 2.22min.

    Intermediate 34: 1-Ethyl-N-(2-hydroxy-1-methylethyl)-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide



    [0400] 

    Intermediate 17 (0.1g, 0.34mmol), EDC (0.066g, 0.34mmol) and HOBT (0.05g, 0.37mmol) were suspended in DMF (2ml) and stirred at room temperature under nitrogen for 15 min. 2-aminopropan-1-ol (0.026g, 0.34mmol) and triethylamine (0.036g, 0.36mmol) were added and the mixture was stirred at room temperature under nitrogen for 6 hours. Solvents were removed in vacuo and the residue partitioned between DCM and water. The organic layer was concentrated and applied to an SPE cartridge (aminopropyl, 5g), which was eluted with methanol. Concentration in vacuo afforded Intermediate 34 (0.095g). LCMS showed MH+ = 348, TRET = 2.15min.

    Intermediate 35: Ethyl 4-ethoxy-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0401] 



    [0402] Sodium (0.55g, 23.7mmol) was added portionwise to anhydrous ethanol (25ml) at 20 °C under an atmosphere of nitrogen. After stirring for 1 hour the solution was added to Intermediate 1 (4.622g, 18.22mmol) and the reaction mixture heated at reflux for 2 hours. The mixture was evaporated in vacuo and the residue partitioned between dichloromethane and water. The organic phase was washed with brine, dried (Na2SO4) and evaporated in vacuo. The residue was purified on SPE cartridges (silica, 4 x 20g) eluting with dichloromethane, ethyl acetate:petroleum ether (1:4, 1:2 then 1:1) followed by ethyl acetate). Appropriate fractions were combined and evaporated in vacuo to afford Intermediate 35 as white solid (4.33g). LCMS showed MH+ = 264, TRET = 2.77min.

    Intermediate 36: Ethyl 4-ethoxy-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0403] 



    [0404] A mixture of Intermediate 35 (1.0g, 3.8mmol) and N-bromosuccinimide (1.49g, 8.4mmol) in carbon tetrachloride (35ml) was heated at reflux for 3 hours. The reaction mixture was cooled in an ice-bath and the precipitate filtered. The filtrate was concentrated in vacuo and the residue dissolved in tetrahydrofuran (12.5ml). Water (3.5ml) and saturated sodium carbonate solution (3ml) were added and the mixture stirred at 20 °C for 18 hours. The reaction was diluted with water and extracted with ethyl acetate. The combined organic phases were dried (Na2SO4) and evaporated in vacuo. The residue was purified on an SPE cartridge (silica, 20g) eluting with dichloromethane, chloroform, then chloroform:methanol (99:1, 49:1, 19:1 then 9:1). Appropriate fractions were combined and evaporated in vacuo to afford Intermediate 36 as an off-white solid (0.45g). LCMS showed MH+ = 236, TRET = 2.46min.

    Intermediate 37: Ethyl 4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0405] 



    [0406] Method 1: Intermediate 36 (0.035g) was placed in a Reactivial™ and treated with 4-aminotetrahydropyran (0.05ml). The mixture was heated at 90 °C for 1.5 hours, then allowed to cool to room temperature and partitioned between chloroform (2ml) and water (1ml). The layers were separated and the organic phase was concentrated. The crude product was purified by mass directed autoprep HPLC to afford Intermediate 37 as an off-white solid (0.011g). LCMS showed MH+= 291; TRET = 2.08 min.

    [0407] Alternative Method 2: Intermediate 36 (2g) was suspended in 4-aminotetrahydropyran (2g), and the mixture was heated at 90 °C for 6 hours. The residual mixture was allowed to cool to room temperature and partitioned between chloroform (50ml) and water (50ml). The phases were separated and the organic phase was evaporated to dryness. The residue was triturated with Et2O (30ml) and the insoluble solid was collected and dried to afford Intermediate 37 as a cream solid (2.24g). LCMS showed MH+= 291; TRER = 2.19 min.

    Intermediate 38: Ethyl 1-n-propyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0408] 



    [0409] Sodium hydride (0.067 g, 60% dispersion in oil) was added to a stirred solution of Intermediate 37 (0.47 g) in DMF (19 ml), followed by n-propyl iodide (0.17 ml). The mixture was stirred at 23 °C for 16 hours, then concentrated, diluted with chloroform (30 ml) and washed with 1:1 water:brine solution (30 ml), separated and the organic layer concentrated. The residue was purified on a SPE cartridge (silica, 10 g) eluting with 10 ml volumes of dichloromethane, 1:1 diethyl ether:cyclohexane, and diethyl ether. The combined 1:1 diethyl ether: cyclohexane, and diethyl ether, fractions were concentrated to give Intermediate 38 as a clear gum (0.23 g). LCMS showed MH+ = 333; TRET = 3.14 min.

    Intermediate 39: 1-n-Propyl4-(tetrahydro-2H-pyran4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid



    [0410] 



    [0411] 2M-Sodium hydroxide solution (0.7 ml) was added to a stirred suspension of Intermediate 38 (0.23 g) in ethanol (5 ml) and water (1.5 ml). After stirring overnight at room temperature, a further quantity of 2M-sodium hydroxide solution (0.7 ml) was added, and the reaction mixture was heated at 43 °C for 2.5 hours. The reaction solution was concentrated, diluted with water (5 ml) and acidified with 2M-hydrochloric acid. The resulting precipitate was collected by filtration, washed with water and dried to give Intermediate 39 as a white solid (0.14 g). LCMS showed MH+ = 305; TRET = 2.42min.

    Intermediate 40: N'-Acetyl 1-n-propyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide



    [0412] 



    [0413] Intermediate 40 can be made from Intermediate 39 in a similar way to the process described for Intermediate 4, for example using a similar or the same number of moles of reagents and/or volumes of solvents.

    Intermediate 41: Ethyl 4-[(1-acetyl-4-piperidinyl)amino]-1-ethyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0414] 



    [0415] Intermediate 1 (0.079g, 0.56mmol), Intermediate 25 (0.129g, 0.51mmol) and diisopropylethylamine (0.45ml, 2.55mmol) in acetonitrile (2ml) were heated at 85 °C for 36 hours. The reaction mixture was concentrated in vacuo and the residue partitioned between DCM and water. The phases were separated using a hydrophobic frit (Whatman). The organic phase was evaporated in vacuo and the residue applied to an SPE cartridge (silica, sag). The cartridge was eluted with EtOAc and then DCM / MeOH (1:1). Fractions containing the desired material were combined and concentrated in vacuo to afford Intermediate 41 (0.1 g). LCMS showed MH+ = 360; TRET = 2.63min.

    Intermediate 42: 1-Ethyl-N-[(1R)-2-hydroxy-1-phenylethyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide



    [0416] 



    [0417] Intermediate 17 (0.25g, 0.86mmol), EDC (0.23g, 1.2mmol) and HOBT (0.139g, 1.03mmol) were suspended in DMF (5ml) and the suspension was stirred at room temperature. After 25min, (2R)-2-Amino-2-phenylethanol (0.13g, 0.95mmol, commercially available from Aldrich) was added, and the mixture was stirred at room temperature for 20 hours. Solvents were removed in vacuo and the residue was dissolved in DCM (50ml) and washed successively with water (25ml) and 5% sodium hydrogen carbonate solution (25ml). The organic layer was dried over anhydrous sodium sulphate and concentrated in vacuo. The residue was dissolved in dichloromethane (5ml) and applied to a SPE cartridge (silica, 10g), which was eluted with a gradient of ethyl acetate - petroleum ether (1:2, 1:1 and 1:0). Fractions containing the desired material were combined and concentrated in vacuo to afford Intermediate 42 as a white foam (0.318g). LCMS showed MH+ = 410; TRET = 2.55mm.

    Intermediate 43: 1-Ethyl-N-[(1S)-2-hydroxy-1-phenylethyl]-4-(tetrabydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide



    [0418] 



    [0419] Intermediate 43 was prepared from Intermediate 17 and (2S)-2-asnino-2-phenylethanol (commecially available from Lancaster Synthesis) using an analogous method to that for Intermediate 42. LCMS showed MH+ = 410; TRET = 2.55min.

    Intermediate 44 1-Ethyl-N-[(1S)-2-hydroxy-1-(phenylmethyl)ethyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide



    [0420] 



    [0421] Intermediate 44 was prepared from Intermediate 17 and (2S)-2-amino-3-phenyl-1-propanol (commercially available from Aldrich) using an analogous method to that for Intermediate 42. LCMS showed MH+ = 424; TRET = 2.60min.

    Intermediate 45: 1-Ethyl-N-[(1R)-2-hydroxy-1-(phenylmethyl)ethyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide



    [0422] 



    [0423] Intermediate 45 was prepared from Intermediate 17 and (2R)-2-amino-3-phenyl-1-propanol (commecially available from Aldrich) using an analagous method to that for Intermediate 42. LCMS showed MH+ = 424; TRET = 2.59min.

    Intermediate 46: 1-Ethyl-N-[(1S,2R)-2-hydroxy-1-phenylpropyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide



    [0424] 



    [0425] Intermediate 46 was prepared from Intermediate 17 and (1S,2R)-1-amino-1-phenyl-2-propanol hydrochloride (commercially available from Arch Corporation, 100 Jersey Avenue, Building D, New Brunswick, NJ 08901, USA) using an analogous method to that for Intermediate 42. LCMS showed MH+ = 424; TRET = 2.58min.

    Intermediate 47: 1-Ethyl-N-[(2R)-2-hydroxy-2-phenylethyl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide



    [0426] 



    [0427] Intermediate 47 was prepared from Intermediate 17 and (1R)-2-amino-1-phenylethanol (commercially available from Aldrich) using an analogous method to that for Intermediate 42. LCMS showed MH+ = 410; TRET = 2.62min.

    Intermediate 48: 1-Ethyl-N-[(2S)-2-hydroxy-2-phenylethyl]-4-(tetrahydro-1H-pyran4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxanitide



    [0428] 



    [0429] Intermediate 48 was prepared from Intermediate 17 and (1S)-2-amino-1-phenylethanol (commercially available from Fluka) using an analogous method to that for Intermediate 42. LCMS showed MH+ = 410; TRET = 2.62min.

    Intermediate 49: 1-Ethyl-N-(2-hydrogy-1,1-dimethylethyl)-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide



    [0430] 



    [0431] Intermediate 49 was prepared from Intermediate 17 and 2-amino-2-methyl-1-propanol (commercially available from Aldrich) using an analogous method to that for Intermediate 42. LCMS showed MH+ = 362; TRET = 2.28min.

    Reference Intermediate 50: N-methyltetrahydro-2H-pyran-4-amine



    [0432] 



    [0433] Can be prepared from tctrahydro-4H-pyran-4-one (commercially available from e.g. Sigma Aldich; CAS (Chemical Abstracts) Registry Number 29943-42-8) according to the procedure described by H.Hashimoto el al. in Organic Process Research and Development 2002, 6, 70.

    Reference Intermediate 51: Ethyl 1-ethyl-4-[methyl(tetrahydro-2H-pyran-4-yl)amino]-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0434] 



    [0435] Intermediate 1 (1.2g, 4.76mmol), Reference Intermediate 50 (0.79g, 5-2mmol) and diisopropylethylamine (4ml, 24mmol) in MeCN (8ml) was heated at 70 °C for 24 hours. The solvent was removed in vacuo and the residue partitioned between DCM and water. The organic phase was concentrated in vacuo and the residue chromatographed on silica (50g) eluting with cyclohexane:ethyl acetate (2:1 followed by 1:1 then 1:2). Appropriate fractions were combined and evaporated to give Reference Intermediate 51 as a brown oil (1.21g). LCMS showed MH+ = 334; TRET = 2.61min.

    Reference Intermediate 52: 1-Ethyl-4-[methyl(tetrahydro-2H-pyran-4-yl)amino]-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid



    [0436] 



    [0437] Sodium hydroxide (0.43g, 10.8mmol) was added to a solution of Reference Intermediates 51 in ethanol (10ml, 95%). The reaction mixture was heated at 50 °C for 18 hours. The solvent was evaporated in vacuo and the residue dissolved in water and acidified to pH 3 by the addition of aqueous hydrochloric acid, The solution was extracted with DCM. The organic phase was separated using a hydrophobic frit (Whatman PTFE Folter Media with Polypropylene Housing 5µM pore size) and the solvent evaporated in vacuo to give Reference Intermediate 52 as a white solid (0.65g). LCMS showed MH+ = 305; TRET = 1.97min.

    Reference Intermediate 53: N'-Acetyl-1-ethyl-4-[methyl(tetrahydro-2H-pyran-4-yl)amino]-1H-pyrazolo[3,4-b]pyridine-5-carboydrazide



    [0438] 



    [0439] Reference Intermediate 53 was prepared from Reference Intermediate 52 using an analogous method to that for Intermediate 4. LCMS showed MH+ = 361; TRET = 1.9lmin.

    Intermediate 54: trans-4-Aminocyclohexanol



    [0440] 



    [0441] Commercially available from e.g. Acros. CAS (Chemical Abstracts) Registry Number 27489-62-9.

    Intermediate 55: Tetrahydro-2H-pyran-3-amine hydrochloride



    [0442] 



    [0443] Prepared as described in Anales Die Quimica, 1988, 84, 148.

    Intermediate 56: 4-Aminocyclohexanone hydrochloride



    [0444] 



    [0445] A solution of hydrogen chloride in dioxan (0.5ml), 2.0mmol, 4M) was added to a stirred solution of tert-butyl 4-oxocyclohexylcarbamate (0.043g, 0.20mmol, commercially available from Astatech Inc., Philadelphia, USA) in dioxan (0.5ml) and the mixture was stirred at room temperature. After 1h, the reaction mixture was evaporated to give Intermediate 56 as a cream solid (34mg), 1H NMR (400MHz in d6-DMSO, 27°C, δppm) 8.09 (br. s, 3H), 3.51 (tt, 11, 3.5Hz, 1H), 2.45 (m, 2H, partially obscured), 2.29 (m, 2H), 2.16 (m, 2H), 1.76 (m, 2H).

    Reference Intermediate 57: N-Propyltetrahydro-2H-pyran-4-amine



    [0446] 



    [0447] Can be prepared from tetrahydro-4H-pyran-4-one (commercially available from e.g. Sigma Aldich CAS 29943-42-8) as described by C. Zagar in WO 99/07702.

    Intermediate 58: Ethyl 4-chloro-1-ethyl-6-methyl-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0448] 



    [0449] A mixture of 5-amino-1-ethylpyrazole (1.614g, 14.5mmol) and diethyl 2-(1-ethoxyethylidene)malonate (3.68g, 16.Ommol, as described by P.P.T. Sah, J. Amer. Chem. Soc., 1931, 53, 1836) was heated at 150 °C under Dean Stark conditions for 5 hours. Phosphorous oxychloride (25ml) was carefully added to the mixture and the resulting solution was heated at 130 °C under reflux for 18 hours. The mixture was concentrated in vacuo, then the residual oil was carefully added, with cooling, to water (100ml). The resulting mixture was extracted with DCM (3x100ml) and the combined organic extracts were dried over anhydrous sodium sulphate and concentrated in vacuo. The residual oil was purified by Biotage chromatography (silica, 90g) eluting with ethyl acetate-petrol (1:19). Fractions containing the desired product were combined and concentrated in vacuo to afford Intermediate 58 (1.15g). LCMS showed MH+ = 268; TRET = 3.18min.

    Intermediate 59: Ethyl 1-ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylate



    [0450] 



    [0451] 4-Aminotetrahydropyran hydrochloride (Intermediate 21, 0.413g, 3.0mmol) was added to a mixture of Intermediate 58 (0.268g, 1.0mmol) and N,N-diisopropylethylamine (0.87ml), 5.0mmol) in acetonitrile (3ml). The resulting mixture was heated at 85°C for 24 hours. Volatiles were removed in vacuo and the residue was dissolved in chloroform (1.5ml) and applied to a SPE cartridge (silica, 5g). The cartridge was eluted successively with Et2O, EtOAc and EtOAc-MeOH (9/1). Fractions containing the desired product were combined and concentrated in vacuo to give the desired product contaminated with starting material (Intermediate 51). Further purification using a SPE cartridge (silica, 5g) eluting with ethyl acetate-cyclohexane (1:3) afforded Intermediate 59 (0.248g). LCMS showed MH+ = 333; TRET = 2.75min.

    Intermediate 60: 1-Ethyt-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxylic acid



    [0452] 



    [0453] 2M-Sodium hydroxide solution (0.75ml, 1.5mmol) was added to Intermediate 59 (0.248g, 0.75mmol) in ethanol (2ml), and the mixture was heated at reflux for 16 hours. The reaction mixture was concentrated, diluted with water (1ml) and acidified with 2M-hydrochloric acid (0.75ml) to precipitate a solid which was collected by filtration to afford Intermediate 60 (0.168g). LCMS showed MH+ = 305; TRET = 1.86min.

    Intermediate 61: N'-(2,2-Dimethylpropanoyl)-1-ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylanfno)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide



    [0454] 



    [0455] Intermediate 60 (0.255g, 0.84mmol), EDC (0.225g, 1. 17mmol) and HOBT (0.136g, 1.0mmol) in DMF (5ml) was stirred at 20°C for 75 minutes. Pivalic acid hydrazide (0.107g, 0.92mmol) was added and stirring continued for 18 hours. The reaction mixture was concentrated in vacuo and the residue partitioned between DCM and water. The organic phase was washed with aqueous sodium hydrogen carbonate then evaporated in vacuo to afford Intermediate 61 as a white solid (0.27g).). LCMS showed MH+ = 403; TRET= 2.13man.

    Intermediate 62: 1,1-Dimethylethyl 2-{[1-ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}hydrazinecarboxylate



    [0456] 



    [0457] Intermediate 60 (0.253g, 0.83mmol), EDC (0.223g, 1.17mmol) and HOBT (0.135g, 1.0mmol) in DMF (5ml) was stirred at 20 °C for 30 minutes. t-Butyl carbazate (0.11 0g 0.83mmol) was added and stirring continued for 18 hours. The reaction mixture was concentrated in vacuo and the residue dissolved in DMF (5ml) additional EDC (0.159g0) and HOBT (0.112g) added. After 30 minutes t-butyl carbazate (0.019g) was added and stirring continued for 18 hours. The reaction was concentrated in vacuo and the residue partitioned between DCM and water. The organic phase was washed with aqueous sodium hydrogen carbonate then evaporated in vacuo. The material was applied to a SPE cartridge (silica, 10g) and eluted with cyclohexane: ethyl acetate (1:1 followed by 2:1) to afford Intermediate 62 as a white solid (0.19g). LCMS showed MH+ = 419; TRET = 2.35min.

    Intermediate 63: 1-Ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide hydrochloride



    [0458] 



    [0459] Intermediate 62 (0.19g, 0.46mmol) was dissolved in 4M hydrogen chloride in dioxane (5ml) and the reaction mixture stirred overnight at 20 °C. Concentration in vacuo afforded Intermediate 63 as a white solid (0.161g). LCMS showed MH+ = 319; TRET = 1.72min.

    Intermediate 64: 1-Ethyl-6-methyl-4-(tetrahydro-2H-pyran-4-ylamino)-N'-(tetrahydro-2H-pyran-4-ylcarbonyl)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide



    [0460] 



    [0461] Intermediate 30 (0.06g, 0.45mmol) and TBTU (0. 146g, 0.45mmol) in DMF (5ml) was stirred at 20 °C for 30 minutes. A mixture of Intermediate 63 (0.16g, 0.45mmol) and diisopropylethylamine (0.32ml, 1.82mmol) in DMF (1ml) was added and the reaction mixture stirred overnight under nitrogen. The reaction was concentrated in vacuo and the residue partitioned between DCM and water. The phases were separated using a hydrophobic frit (Whatman PTFE Folter Media with Polypropylene Housing 5µM pore size) and the organic phase evaporated in vacuo. The residue was applied to an SPE cartridge (aminopropyl, 10g) and eluted with MeOH. Appropriate fractions were concentrated in vacuo then applied to an additional SPE cartridge (silica, 2g) which was eluted sequentially with a gradient of MeOH in DCM (i) 2%, (ii) 4%, (iii) 6% and (iv) 10%. Fractions containing the desired material were combined and concentrated in vacuo to afford Intermediate 64 as a white solid (0.048g). LCMS showed MH+ = 431; TRET = 1.87min.

    Intermediate 65: N'-(Cyclobutylcarbonyl)-1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide



    [0462] 



    [0463] TBTU (0.050g, 0.15mmol) and diisopropylethylamine (0.04ml), 0.26 mmol) in DMF (0.5ml) was added to cyclobutylcarboxylic acid (RYCOOH, 0.015g, 0.15mmol). The reaction mixture was stirred for 40 minutes at 20°C. A mixture of Intermediate 19 (0.045g, 0.13mmol) and diisopropylethylamine (0.04ml, 0.26mmol) in DMF (0.5ml) was added and the reaction mixture stirred for 18h. The solvent was removed in vacuo and the residue applied to a SPE cartridge (aminopropyl, 2g). The cartridge was eluted with methanol to afford Intermediate 65 (0.052g). LCMS showed MH+ = 387; TRET = 2.28min.

    [0464] Similarly prepared using the same or similar numbers of moles of reagents and/or volumes of solvents were the following:
     RYCOOHSource of AcidMH+TRET (min)
    Intermediate 66

    Sigma-Aldrich 416 2.03
    Intermediate 67

    Sigma-Aldrich 404 2.01
    Intermediate 68

    HCl Salt: Maybridge, or DE10008089 430 1.89
    Intermediate 69

    Interchim Intermediates 429 2.35
    Intermediate 70

    Sigma-Aldrich, or J. Org. Chem., 1997, 62, 5144 415 2.12
    Intermediate 71

    Sigma-Aldrich 403 2.11
    Intermediate 72

    Sigma-Aldrich 434 2.15
    Intermediate 73

    ChemPacific 401 2.46
    Intermediate 74

    Sigma-Aldrich 418 2.06
    Intermediate 75

    Astatech, or J. Med. Chem., 1993, 36, 2300 431 2.18
    Intermediate 76

    Synthesis, 1971,258;401 2.35 or WO 03/082190 401 2.35
    Intermediate 77

    Eur. J. Med. Chem., 1992 37, 581 414 2.30 414 2.30
    Intermediate 78

    Indian J. Chemistry 2002, 41B, 1093 413 2.24 458 2.24
    Intermediate 79

    Lancaster Synthesis 458 2.18

    Intermediate 80: (1E/Z)-N-hydroxy-2-(4-methyl-1-piperazinyl)ethanimidamide



    [0465] 



    [0466] (4-Methyl-1-piperazinyl)acetonitrile (1.08g, 7.7mmol) (J. Med. Chem., 1999, 42, 2870) was added to a suspension of potassium carbonate (3.2g, 23.1mmol) and hydroxylamine hydrochloride (1.06g, 15.4mmol) in ethanol (10ml). The reaction mixture was heated at reflux for 9 hours then allowed to cool. The reaction was filtered and the solvent evaporated in vacuo to afford Intermediate 80 (1.53g). 1H NMR (400MHz in d6-DMSO, 27°C, δppm) 9.02 (br s, 1H), 5.17 (br s, 2H), 2.78 (s, 2H), 2.31 (br s , 8H), 2.13 (s, 3H).

    Intermediate 81: 4-Fluoro-N-hydroxybenzenecarboximidamide



    [0467] 



    [0468] Commercially available from Sigma-Aldrich, CAS (Chemical Abstracts) Registry Number 22179-78-8.

    Intermediate 82: (1E/Z)-N-hydroxy-3-oxo-3-(1-pyrrolidinyl)propanimidamide



    [0469] 



    [0470] Commercially available from the Maybridge Chemical Company, CAS (Chemical Abstracts) Registry Number 57399-51-6.

    Intermediates 83 and 84



    [0471] The structures of Intermediates 83 and 84 and their preparation are as follows:


    Intermediate 83: {5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}acetic acid



    [0472] 



    [0473] Anhydrous hydrogen chloride in dioxane (8ml, 4M solution) was added to Intemediate 84 (0.807g, 1.88mol). The reaction mixture was stirred overnight at room temperature then evaporated in vacuo. The residue was suspended in ether and the mixture filtered to give Intermediate 83 as a brown solid (0.525g). LCMS showed MH+ = 373; TRET = 2.62min.

    Intermediate 84: 1,1-Dimethylethyl {5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}acetate



    [0474] 



    [0475] Diisopropyethylamine (8.3ml, 47.5mmol) was added to a mixture of Intermediate 17 (2.76g, 9.5mmol), TBTU (3.050g, 9.5mmol) and hydroxybenzotriazole (1.28g, 9.5mmol) in N,N-dimethylformamide (40ml) at room temperature. After stirring for 10 minutes Intermediate 85 (2.318g, 13.3mmol) was added. The reaction mixture was srirred for 50 minutes then 1,1'-carbonyldiimidazole (1.54g, 9.5mmol) was added and the reaction heated at 100 °C for 16 hours. The solvent was removed in vacuo and the residue partitioned between dichloromethane and water. The organic phase was washed with aqueous sodium hydrogen carbonate (5%) then dried (Na2SO4) and evaporated in vacuo. The residue was purified by chromaography using the Biotage system (100g, silica) eluting with cyclohexane:ethyl acetate (1:1). Intermediate 84 was obtained a brown solid (0.97g). LCMS showed MH+ = 429 TRET = 3.26min.

    Intermediate 85: 1,1-Dimethylethyl (3E/Z)-3-(hydroxyamino)-3-iminopropanoate



    [0476] 



    [0477] The reaction scheme was as follows:



    [0478] A solution of sodium methoxide in methanol (50ml, 0.5M) was added to hydroxylamine hydrochloride (1.78g, 25.62mmol) at room temperature. After stirring for 15 minutes the solution was filtered and the filtrate added to t-butyl cyanoacetate (3.0g, 21.25mmol), available from Aldrich). The solution was refluxed for 1.75 hours then cooled and evaporated in vacuo. The residue was partitioned between ethyl acetate and water. The aqueous phase was extracted with ethyl acetate and the combined organic phases washed with brine, dried (MgSO4) and evaporated in vacuo. The residue was suspended in cyclohexane: ether (1:1) then filtered to give Intermediate 85 as a white solid (1.883g). 1H NMR (400MHz in CDCl3, 27°C, δppm) 8.34 (br s, 1H), 5.05 (br s, 2H), 3.09 (s, 2H), 1.47 (s, 9H).

    Intermediate 86: N"-{[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]carbonyl}-N,N,N',N'-tetramethylcarbonohydrazonic diamide



    [0479] 



    [0480] Intermediate 19 (0.1g, 0.29mmol), TBTU (0.094g, 0.29mmol) and diisopropylethylamine (0.204ml, 1.17mmol) in N,N-dimethylformamide (1ml) were stirred at room temperature for I hour. The solvent was evaporated in vacuo and the residue dissolved in methanol and applied to an SPE cartridge (aminopropyl, 5g). The cartridge was eluted with methanol and appropriate fractions evaporated in vacuo to give Intermediate 86 as a yellow solid (0.113g). LCMS showed MH+ = 403; TRET = 1.99min.

    Intermediate 87: Ethyl (2-methyl-1,3-thiazol-4-yl) acetate



    [0481] Prepared as described by K. Arakawa et. al., Chem Pharm Bull, 1972, 20 (5), 1041


    Intermediate 88: 2-Methyl-1,3-thiazol-4-yl acetic acid



    [0482] 



    [0483] To a solution of Intermediate 87 (6g, 32.4mmol) in dioxan (15ml) was added a solution of lithium hydroxide monohydrate (1.53g, 36.4mmol) in water (15ml). The mixture was stirred for 17h, then washed with diethyl ether (20ml), then with ethyl acetate (20ml) and acidified with concentrated hydrochloric acid under ethyl acetate (50ml). The combined aqueous phases were adjusted to pH 2.7 by addition of sodium bicarbonate and extracted with further ethyl acetate (2x50ml). The combined organic phases were washed with water (20ml) and saturated brine (20ml), then concentrated in vacuo to afford Intermediate 88 as a white solid (1.69g). 1H NMR (400MHz in CDCl3, 27°C, δppm) 2.74 (s, 3H), 3.85 (s, 2H), 5.8-6.2 (br, s, 1H), 7.02 (s, 1H).

    Intermediate 89: 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-N'-(1H-1,2,3-triazol-1-ylacetyl)-1H-pyrazolo[3,4-b]pyridine-5-carbohydrazide



    [0484] 


    General Procedure for Intermediates 89 to 114:



    [0485] A mixture of carboxylic acid RYCO2H (0.2mmol), diisopropylethylamine (0.105ml, 0.6mmol) and TBTU (0.071g, 0.22mmol) in N,N-dimethylformamide (0.5ml) was allowed to stand for 10 minutes. A mixture of Intermediate 19 (0.2mmol) and diisopropylethylamine (0.035ml, 0.2mmol) in N,N-dimethylformamide (0.5ml) was added. After agitation the reactions were allowed to stand for 16 hours. The solvent was removed in vacuo and residue was applied to an SPE cartridge (aminopropyl, 0.5g). The cartridge was eluted with chloroform, ethyl acetate:chloroform (1:1), ethyl acetate, ethyl acetate:methanol (9:1, 2ml). Appropriate fractions were evaporated in vacuo to afford the Intermediates below.
    Intermediate NumberRYSource of Carboxylic acid RYCO2HMH+TRET (min)
    89

    ChemPacific Ltd 413 2.17
    90

    SPECS Fleminglaan 16 2289 CP Rijswijk The Netherlands 457 2.3
    91

    Advanced Synthesis P.O. Box 437920 San Ysidro, California 92173 United States 412 2.4
    92

    Microchemistry Ltd, Shosse Entusiastov 56, Moscow 1111123, Russia 413 2.25
    93

    Aldrich 452 2.59
    94

    Maybridge Chemical Company Ltd., Trevillett, Tintagel, Cornwall PL34 0HW, United Kingdom 414 2.2
    95

    Described by R. Raap et. al., US3271407A 429 2.33
    96

    Aldrich 427 2.32
    97

    Aldrich 465 2.21
    98

    Intermediate 88 443 2.33
    99

    Peakdale Molecular Ltd, Peakdale Science Park, Sheffield Road, Chapel-en-le-Frith, High Peak SK23 0PG, UK 485 2.47
    100

    J. Chem. Soc., Perkin Trans II, 1993,4, 741-8 429 2.19
    101

    Lancaster Synthesis 413 2.42
    102

    J. Chem. Soc. Perkin Trans. 1, 1976, 9, 994-7 427 2.35
    103

    Microchemistry Ltd, Shosse Entusiastov 56, Moscow 1111123, Russia 457 2.32
    104

    Microchemistry Ltd, Shosse Entusiastov 56, Moscow 1111123, Russia 429 2.13
    105

    Microchemistry Ltd, Shosse Entusiastov 56, Moscow 1111123, Russia 427 - 2.11
    106

    Microchemistry Ltd, Shosse Entusiastov 56, Moscow 1111123, Russia 441 2.35
    107

    Sigma Chemical Company 417 2.11
    108

    T. J. Guzi et.al., WO 03/022835 471 2.22
    109

    Aldrich 437 2.7
    110

    Aldrich 423 2.6
    111

    Aldrich 437 2.8
    112

    Aldrich 437 2.7
    113

    ACB Blocks Ltd or Described by Rzeszotarski, W.J. in WO 93/05772 451 2.7
    114

    Aldrich 501/503 2.8

    Intermediate 115: 4-(Aminomethyl)benzamide



    [0486] 



    [0487] Can be prepared according to the procedure described by L.W.Jones et. al. WO 02/085860.

    Intermediate 116: 1,1-Dimethylethyl [(2Z)-2-(hydroxyamino)-2-iminoethyl]carbamate



    [0488] 



    [0489] Prepared from commercially available N-(tert-Butoxycarbonyl)-2-aminoacetonitrile as decribed by M. Schwarz et. al. WO 02/102799.

    Intermediate 117: 1,1-Dimethylethyl ({5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)carbamate



    [0490] 



    [0491] Diisopropylethylamine (6.0ml, 34.4mmol) was added to a stirred mixture of Intermediate 17 (2.0g, 6.89mmol), TBTU (2.212g, 6.89mmol) and HOBT (0.931g, 6.89mmol) in dry dimethylformamide (45ml). After 10min, the resulting clear solution was treated with Intermediate 116 (1.89g, 10mmol). The reaction mixture was stirred at room temperature for 2h. DBU (5.14ml, 34.5mmol) was added, and the reaction mixture was heated at 80°C. After 3.5h at 80°C, the reaction mixture was evaporated in vacuo, and the residue was dissolved in dichloromethane (150ml)) and washed successively with 5% sodium hydrogen carbonate (50ml) and water (50ml). The organic solution was dried over anhydrous sodium sulphate and evaporated to give the crude product. Purification by Biotage chromatography (silica, 100g) eluting with ethyl acetate-petroleum ether (1:1) afforded Intermediate 117 as a white solid (2.70g). LCMS showed MH+ =444, TRET = 3.06min.

    Intermediate 118: 5-[3-(Aminomethyl)-1,2,4-oxadiazol-5-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0492] 



    [0493] Trifluoroacetic acid (5ml) was added to a stirred solution of Intermediate 117 (1.774g, 4.0mmol) in dry dichloromethane (20ml) at 0°C. After 2h, the reaction mixture was neutralised by careful addition of 5% sodium hydrogen carbonate solution (150ml) and solid sodium hydrogen carbonate. The resulting mixture was extracted with chloroform (2 x 100ml). The combined organic extracts were dried over anhydrous sodium sulphate and evaporated to afford Intermediate 118 as a white solid (1.358g). LCMS showed MH+ =344, TRET = 1.95min.

    Intermediate 119: 4-Chloro-N-({5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)butanamide



    [0494] 



    [0495] 4-chlorobutanoyl chloride (0.12mmol) was added to a stirred solution of Intermediate 118 (0.1mmol) and diisopropylethylamine (0.3mmol) in chloroform (1ml) at room temperature. After stirring at room temperature for 16h, the reaction mixture was applied to a SPE cartridge (aminopropyl, 2g) and the cartridge was eluted sequentially with chlororm, ethyl acetate and methanol. Fractions containing the desired product were combined and blown down under nitrogen. The resulting residue was further purified on a SPE cartidge (silica, 1g) eluting with a gradient of 30-100% ethyl acetate in petroleum ether to afford Intermediate 119 as a white solid (45mg).). LCMS showed MH+ =448, TRET = 2.77min.

    Intermediate 120: 5-Chloro-N-({5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)pentanamide



    [0496] 



    [0497] 5-chloropentanoyl chloride (0.12mmol) was added to a stirred solution of Intermediate 118 (0.1mmol) and diisopropylethylamine (0.3mmol) in chloroform (1ml) at room temperature. After stirring at room temperature for 16h, the reaction mixture was applied to a SPE cartridge (aminopropyl, 2g) and the cartridge was eluted sequentially with chlororm, ethyl acetate and methanol. Fractions containing the desired product were combined and blown down under nitrogen. The resulting residue was further purified on a SPE cartidge (silica, 1g) eluting with a gradient of 30-100% ethyl acetate in petroleum ether to afford Intermediate 120 as a white solid (46mg). LCMS showed MH+ =462, TRET = 2.86min.

    Intermediate 121: (1E/Z)-N-hydroxy-2-(4-morpholinyl)propanimidamide



    [0498] 



    [0499] Prepared from α-methyl-4-morpholineacetonitrile using a similar process to that described for any of Intermediates 9, 22 or 80 using similar or the same number of moles of reagents and/or volumes of solvents. 1H NMR (27 °C, d4-MeOH) 3.70-3.60 (m, 5H), 3.13-3.07 (m, 2H), 2.83-2.76 (m, 2H), 1.84 (d, J = 5Hz, 3H)

    [0500] α-Methyl-4-morpholineacetonitrile can be prepared according to the procedure described by H.R.Henze et. al. J. Am. Chem. Soc 1957, 79, 6230.

    Intermediate 122: (1E/Z)-2-cyclohexyl-N-hydroxyethanimidamide



    [0501] 



    [0502] Can be prepared from cyclohexylacetic acid (commercially available from e.g. Aldrich) according to the procedure described by T.R. Alessi et al.in US 4895860.

    Intermediate 123: 1,1-Dimethylethyl 4-[(2Z)-2-(hydroxyamino)-2-iminoethyl]-1-piperidinecarboxylate



    [0503] 



    [0504] prepared from 1,1-dimethylethyl 4-(cyanomethyl)-1-piperidinecarboxylate using a similar process to that described for any of Intermediates 9, 22 or 80 using similar or the same number of moles of reagents and/or volumes of solvents.

    [0505] 1,1-dimethylethyl 4-(cyanomethyl)-1-piperidinecarboxylate can be prepared from commercially available 1,1-dimethylethyl 4-oxo-1-piperidinecarboxylate according to the procedure described by A.M.Wilson in WO 00/006159.

    Intermediate 124: 1,1-Dimethylethyl 4-({5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-1-piperidinecarboxylate



    [0506] 



    [0507] A mixture of Intermediate 16 (0.064g, 0.2mmol), Intermediate 80 (0.257g, 1mmol), a solution of sodium ethoxide in EtOH (0.19ml, 21% solution) and powdered 4Å molecular sieves (0.38g) in EtOH (2ml) were stirred at 82 °C under an atmosphere of nitrogen for 18 hours. Additional sodium ethoxide in ethanol (0.19ml, 21% solution), molecular sieves (0.38g) and ethanol (4ml) were added and the reaction heated for a further 72 hours. The reaction mixture was filtered, the solvent was evaporated in vacuo and the residue was applied to an SPE cartridge (silica, 2g). The cartridge was eluted with cyclohexane: ethyl acetate (4:1, 2:1, 1:1), then ethyl acetate to afford Intermediate 124 as a colourless oil (0.052g). LCMS showed MH+ = 512; TRET = 3.51min.

    Intermediate 125: 1-Ethyl-5-[3-(4-piperidinylmethyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine hydrochloride



    [0508] 



    [0509] A solution of hydrogen chloride in dioxane (1ml) was added to Intermediate 124 (0.052g, 0.1mmol) and the reaction mixture stirred at 20 °C for 2 hours. The solution was evaporated in vacuo to afford Intermediate 125 as a yellow solid (0.047g). LCMS showed MH+ = 412; TRET = 2.21 min.

    Intermediate 126: N-Hydroxy-1-(phenylsulfonyl)cyclopropanecarboximidamide



    [0510] 



    [0511] Prepared from 1-(phenylsulphonyl)cyclopropoanecarbonitrile (commercially available from Menai Organics Ltd, Menai Technology Centre, Deiniol Roas, Bangor, Gwynedd, Wales, LL57 UP, United Kingdom or described in Bull. Chem. Soc. Jpn. 1985 58(2), 765) using a similar process to that described for any of Intermediates 9, 22 or 80 using similar or the same number of moles of reagents and/or volumes of solvents. LCMS showed MH+ = 241; TRET = 1.71min.

    Intermediate 127: (1E/Z)-N-Hydroxy-2-phenylethanimidamide



    [0512] 



    [0513] Commercially available from Maybridge Chemical Company Ltd, Trevillett, Tintagel, Cornwall, PL340HW, United Kingdom.

    Intermediate 128: (1E/Z)-N-Hydroxy-2-phenylpropanimidamide



    [0514] 



    [0515] Can be prepared from a-methylphenylacetonitirile according to the procedure described by J. Rheineimer EP 323864.

    Intermediate 129: (1E/Z)-N-Hydroxy-2-[4-(methyloxy)phenyl]ethanimidamide



    [0516] 



    [0517] Commercially available from Exploratory Library, Ambinter, 46 quai Louis Bleriot, Paris, F-75016, France.

    Intermediate 130: (1E/Z)-N-Hydroxy-2-[3-(methyloxy)phenyl]ethanimidamide



    [0518] 



    [0519] Can be prepared from according to the procedure described by S.Borg et. A1 European J.Med Chem. 1993, 28(10,) 801.

    Intermediate 131: (1E/Z)-2-[4-(Dimethylamino)pbenyl]-N-hydroxyethanimidamide



    [0520] 



    [0521] Prepared from 4-(dimethylamino)benzeneacetonitrile (described by Borovicka et al. Collect. Czech. Chem. Commun 1955, 20, 437) using a similar process to that described for any of Intermediates 9, 22 or 80 using similar or the same number of moles of reagents and/or volumes of solvents. LCMS showed MH+ = 194; TRET = 0.38min.

    Intermediate 132: (1E/Z)-2-[3-(Dimethylamino)phenyl]-N-hydroxyethanimidamide



    [0522] 



    [0523] Prepared from 3-(dimethylamino)benzeneacetonitrile (described by M.L. Sznaidman et al. Bioorganic Medicinal Chemistry Letters 1996, 6(5), 565) using a similar process to that described for any of Intermediates 9, 22 or 80 using similar or the same number of moles of reagents and/or volumes of solvents. LCMS showed MH+ = 194; TRET = 0.46min

    Intermediate 133: (1E/Z)-N-Hydroxy-2-(phenyloxy)ethanimidamide



    [0524] 



    [0525] Commercially available from Pfaltz & Bauer Inc.

    Intermediate 134: (1E/Z)-N-hydroxy-2-(5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyridin-3-yl)ethanimidamide



    [0526] 



    [0527] Prepared from 5,6,7,8-tetrahydro-1,2,4-triazolo[4,3-a]pyridine-3-acetonitrile (commercially available from Interchim, 213 Avenue Kennedy, BP 1140, Montlucon, Cedex 03103, France or Exploratory Library, Ambinter, 46 quai Louis Bleriot, Paris, F-75016, France) using a similar process to that described for any of Intermediates 9, 22 or 80 using similar or the same number of moles of reagents and/or volumes of solvents.
    LCMS showed MH+ = 198; TRET = 0.32min.

    Intermediate 135: (1E/Z)-N-Hydroxy-2-(4-phenyl-1-piperazinyl)ethanimidamide



    [0528] 



    [0529] Prepared from 4-phenyl-1-piperazineacetonitrile (commercially available from Interchim, 213 Avenue Kennedy, BP 1140, Montlucon, Cedex 03103, France or Exploratory Library, Ambinter, 46 quai Louis Bleriot, Paris, F-75016, France) using a similar process to that described for any of Intermediates 9, 22 or 80 using similar or the same number of moles of reagents and/or volumes of solvents. LCMS showed MH+ = 235; TRET = 1.09min.

    Intermediate 136: 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide



    [0530] 



    [0531] A solution of Intermediate 17 (3.263g, 11.25mmol) in thionyl chloride (17ml) was heated at 60 °C for 2 hours. The solution was concentrated in vacuo and then co-evaporated with dichloromethane. The residue was suspended in a solution of ammonia in dioxane (45ml, 0,5M solution) and the resultant mixture stirred for 18 hours. After concentration in vacuo the residue was re-suspended in ammonia in dioxane (45ml, 0.5M) and stirred for a further 16 hours. The solvent was removed in vacuo and the solid suspended in a mixture of dichloromethane (40ml) and water (40ml). The solid was filtered, washed with water and dried in vacuo over P2O5 to afford Intermediate 136 as a cream solid (2.50g). LCMS showed MH+ = 290; TRET = 2.12min

    Intermediate 137: 1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carbonitrile



    [0532] 



    [0533] Burgess Reagent 4.53g, 19.0mmol) was added to a suspension of Intermediate 136 (5.0g, 17.3mmol) in THF (80ml). The reaction mixture was stirred at room temperature for 18 hours then a further portion of Burgess Reagent (0.9g, 1.8mmol) was added and stirring continued for 5 hours. The reaction mixture was concentrated in vacuo and the residue partitioned between dichloromethane and water. The organic phase was washed with water, dried and evaporated in vacuo to afford Intermediate 137 as an off-white solid (4.43g). LCMS showed MH+ = 272; TRET = 2.40min

    Intermediate 138: 1-Ethyl-N-hydroxy-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboximidamide



    [0534] 



    [0535] Intermediate 137 (3.50g, 12.9mmol), hydroxylamine hydrochloride (3.30g, 47.8mmol) and sodium hydrogencarbonate (4.01g, 47.8mmol) in EtOH (45ml) were heated at 45 °C for 1.5 hours then at 50 °C for 2.5 hours. The suspension was concentrated in vacuo and the solid stirred in dichloromethane (80ml) for 0.5 hours. The mixture was filtered and the solid stirred in EtOH, the resultant mixture was filtered and the filtrate evaporated. The solid was then washed with dichloromethane three time to afford Intermediate 138 as a white solid (1.62g). LCMS showed MH+ = 305; TRET =1.85min

    Intermediate 139: 1-Ethyl-N-[4-(hydroxymethyl)tetrahydro-2H-pyran-4-yl]-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-carboxamide



    [0536] 



    [0537] Intermediate 139 was prepared from Intermediate 17 and (4-aminoterahydro-2H-pyran-4-yl)methanol (commercially available from PharmaCore Inc., 4170 Mendenhall Oaks Pkwy, Suite 140, High point, NC, USA) using an analogous method to that for Intermediate 42. LCMS showed MH+ = 404, TRET =2.19min.

    Intermediate 140: (R)-(+)-3-Amino tetrahydrofuran 4-toluenesulphonate Commercially available from Fluka Chemie AG, Germany (CAS 111769-27-8)



    [0538] 


    Intermediate 141: (S)-(-)-3-Amino tetrahydrofuran 4-toluenesulphonate



    [0539] Commercially available from E. Merck, Germany; or from E. Merck (Merck Ltd), Hunter Boulevard, Magna Park, Lutterworth, Leicestershire LE17 4XN, United Kingdom (CAS 104530-80-5)


    Intermediate 142: Tetrahydro-2H-thiopyran-4-amine



    [0540] Prepared from commercially available tetrahydrothiopyran-4-one as described by Subramanian et. al., J. Org. Chem., 1981, 46, 4376-4383. Subsequent preparation of the hydrochloride salt can be achieved by conventional means.


    Intermediate 143: Tetrahydro-3-thiopheneamine



    [0541] Prepared in an analogous manner to Intermediate 142 from commercially available tetrahydrothiophene-4-one. The oxime formation is described by Grigg et.al., Tetrahedron, 1991, 47, 4477-4494 and the oxime reduction by Unterhalt et. al., Arch. Pharm., 1990, 317-318.


    Intermediate 144: Tetrahydro-3-thiopheneamine 1,1-dioxide hydrochloride



    [0542] Commercially available from Sigma Aldrich Library of Rare Chemicals (SALOR) (CAS-6338-70-1). Preparation of the hydrochloride salt of the amine can be achieved by conventional means.


    Intermediate 145: Tetrahydro-2H-thiopyran-4-amine-1,1-dioxide hydrochloride



    [0543] Prepared in an analogous manner to Intermediate 11 from commercially available tetrahydrothiophene-4-one. Oxidation to 1,1-dioxo-tetrahydro-1λ6-thiopyran-4-one is described by Rule et. al., in J. Org. Chem., 1995, 60, 1665-1673. Oxime formation is described by Truce et.al., in J. Org. Chem., 1957, 617, 620 and oxime reduction by Barkenbus et. al., J. Am. Chem. Soc., 1955, 77, 3866. Subsequent preparation of the hydrochloride salt of the amine can be achieved by conventional means.


    Intermediate 146: 1,1-Dimethylethyl (4,4-difluorocyclohexyl)carbamate



    [0544] 



    [0545] (Diethylamino)sulphur trifluoride (DAST), (0.06ml, 0.47mmol), was added to a stirred solution of 1,1-dimethylethyl(4-oxocyclohexyl)carbamate, (250mg, 1.17mmol), commercially available from AstaTech Inc., Philadelphia, USA) in anhydrous dichloromethane (5ml) and the mixture was stirred under nitrogen at 20°C. After 22h, the reaction mixture was cooled to 0°C, treated with saturated sodium hydrogen carbonate solution (4ml), and then allowed to warm to ambient temperature. The phases were separated by passage through a hydrophobic frit and the aqueous phase was further extracted with DCM (5ml). The combined organic phases were concentrated in vacuo to give an orange solid (369mg) which was further purified by chromatography using a SPE cartridge (silica, 10g), eluting with DCM to afford Intermediate 62 (140mg) containing 20% of 1,1-dimethylethyl (4-fluoro-3-cyclohexen-1-yl)carbamate. 1H NMR (400MHz in CDCl3, 27°C, δppm)
    Minor component: 55.11 (dm, 16Hz, 1H), 4.56 (br, 1H), 3.80 (br, 1H) 2.45-1.45 (m's, 6H excess), 1.43 (s, 9H). Major component: δ4.43 (br, 1H), 3.58 (br, 1H), 2.45-1.45 (m's, 8H excess), 1.45 (s, 9H).

    Intermediate 147: (4,4-Difluorocyclohexyl)amine hydrochloride



    [0546] 



    [0547] A solution of hydrogen chloride in dioxane (4M, 1.6ml) was added at 20°C to a stirred solution of Intermediate 146 (140mg, 0.6mmol), in dioxane (1.6ml). After 3h, the reaction mixture was concentrated in vacuo to afford Intermediate 147 (96.5mg) containing 4-fluoro-3-cyclohexen-1-amine. 1H NMR (400MHz in d6-DMSO, 27°C, δppm) Minor component: 58.22 (br, 3H excess), 5.18 (dm, 16Hz, 1H), 3.28-3.13 (m, 1H excess), 2.41-1.53 (m's, 6H excess). Major component: 88.22 (br, 3H excess), 3.28-3.13 (m, 1H excess), 2.41-1.53 (m's, 8H excess). Impurities are also present.

    Intermediates 148 to 163: different types of R3R3aNH



    [0548] 
    Intermediate NumberR3R3aNHSource of R3R3aNH
    148

    J. Chem. Soc., Perkin Trans. 1, 1994, 537
    148A as Intermediate 148, but racemic cis-isomer, i.e. racemic cis-(3-hydroxy-cyclohex-1-yl)-amine J. Chem. Soc., Perkin. Trans 1, 1994, 537
    149

    Aldrich; or TCI-America
    150

    US 4219660
    151

    Aldrich
    152

    Aldrich
    153

    Aldrich
    154

    Pfaltz-Bauer
    155

    J. Org. Chem., 1985, 50(11), 1859
    156

    WO 99/12933
    157

    EP 1188744
    158



    Sigma-Aldrich Company Ltd
    159 *

    J. Med. Chem., 1994, 37(17), 2360
    160 *

    Aldrich
    161 *

    Aldrich
    162 *

    Aldrich
    163 *

    Peakdale Molecular Ltd
         
    * For R3R3aNH in Intermediates 159-163, R3R3aNH is the cis or trans isomer, if shown. For Intermediates 161-163, R3R3aNH is usually the 3-amino- or 2-amino-cyclohex-1-ylamine in a racemic form.
    Table of Examples
    Example NumberName
    1 N-Cyclopentyl-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    2 N-Cyclopentyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amine
    3 N-Cyclopentyl-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    4 N-Cyclopentyl-1-ethyl-5-(5-methyl-1,3,4-thiadiazol-2-yl)-1H-pyrazolo[3,4-blpyridin-4-amine
    5 N-Cyclopentyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-thiadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amine
    6 N-Cyclopentyl-1-ethyl-5-(5-isopropyl-1,3,4-thiadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    7 1-Ethyl-N-(4-fluorophenyl)-5-(3-methyl-1,2,4-oxadiazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    8 N-Cyclopentyl-5-(1,3-dimethyl-1H-1,2,4-triazol-5-yl)-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    9 1-Ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine
    10 N-Cyclohexyl-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrszolo[3,4-b]pyridin-4-amine
    11 1-Ethyl-N-isobutyl-5-(6-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    12 1-Ethyl-N-isobutyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-bapyridin-4-amine (Reference Example)
    13 N-Cyclohexyl-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    14 1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine also named: 1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    15 N-[(1R)-1,2-dimethylpropyl]-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    16 N-[(1S)-1,2-dimethylpropyl]-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    17 5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine also named: 5-[5-(1,1-Dimethylethylethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    18 5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-N-cyclohexyl-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amine
    19 5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-N-cyclopentyl-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amine
    20 5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-isobutyl-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    21 5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-N-[(1S)-1,2-dimethylpropyl]-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    22 5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-N-[(1R)-1,2-dimethylpropyl]-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    23 1-Ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine
    24 N-Cyclohexyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amine
    25 1-Ethyl-N-isobutyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyrldin-4-amine (Reference Example)
    26 N-[(1S)-1,2-dimehtylpropyl]-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    27 N-[(1R)-1,2-dimethylpropyl]-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    28 1-Ethyl-5-(3-methyl-1,2,4-oxadiazol-5-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine
    29 1-Ethyl-5-[3-(methoxymethyl)-1,2,4-oxadiazol-5-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine
    30 5-{3-[(Dimethylamino)methyl]-1,2,4-xadiazol-5-yl}-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine
    31 1-Ethyl-5-[3-(morpholin-4-ylmethyl)-1,2,4-oxadiazol-5-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine
    32 5-(5-Cyclopropyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine
    33 N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    34 1-Ethyl-5-[5-(3-methyloxetan-3-yl)-1,3,4-oxadiazol-2-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine
    35 1-Ethyl-5-{5-[(4-methylpiperazin-1-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine also named: 1-Etbyl-5-{5-[(4-methyl-1-piperazinyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    36 5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-isopropyl-1,3,4-oxadiazole-2-carboxamide
    37 4-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-1-methylpyrrolidin-2-one
    38 1-Ethyl-N-tetrahydro-2H-pyran-4-yl-5-(5-tetrahydro-2H-pyran-4-yl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine also named: 1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine
    39 1-Ethyl-5-[5-(morpholin-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine also named: 1-Ethyl-5-[5-(morpholin-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    40 5-[5-(Tert-butoxymethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine
    40A Methyl 2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-4,5-dihydro-1,3-oxazole-4-carboxylate
    41 Methyl 2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxylate
    42 1-Ethyl-5-(4-methyl-4,5-dihydro-1,3-oxazol-2-yl)-N-(tetrahydro-2H-pryan-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    43 1-(n-Propyl)-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    44 1-Ethyl-5-[5-(tetrahydrofuran-2-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    45 1-Ethyl-5-[5-(dimethylamino)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyrm-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    46 1-Ethyl-5-(5-methyl-1,2,4-triazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    47 N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-(3-methyl-1,2,4-oxadiazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    48 N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-[3-(morpholin-4-ylmethyl)-1,2,4-oxadiazol-5-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine
    49 1-Ethyl-5-[(4R)-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    50 1-Ethyl-5-[(4S)-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    51 1-Ethyl-5-[(4S)-4-(phenylmethyl)-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    52 1-Ethyl-5-[(4R)-4-(phenylmethyl)-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    53 1-Ethyl-5-[(4S,5R)-5-methyl-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    54 1-Ethyl-5-[(5R)-5-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    55 1-Ethyl-5-[(5S)-5-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo-[3,4-b]pyridin-4-amine
    56 5-(4,4-Dimethyl-4,5-dihydro-1,3-oxazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    57 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pridin-5-yl]-1,3-oxazole-4-carboxylic acid
    58 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(1-methylethyl)-1,3-oxazole-4-carboxamide
    59 1-Ethyl-5-[4-(4-morpholinylcarbonyl)-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    60 1-Ethyl-N-methyl-5-(5-methyl-1,3,4-oxadiaolo-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    61 trans-4-{[1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-blpyridin-4-yl]amino}cycolohexanol
    62 1-Ethyl-5-(5-methyl-1,3,4-oxadinol-2-yl)-N-(tetrahydro-2H-pyran-3-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    63 4-{[1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-yl]amino}cyclohexanone
    64 1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-n-propyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (Reference Example)
    65 5-[5-(1,1-Dimethylethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-6-methyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    66 1-Ethyl-6-methyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine
    67 5-(5-Cyclobutyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    68 5- {5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-2-pyrrolidinone
    69 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)accetamide
    70 1-Ethyl-5-[5-(1-methyl-2-piperidinyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    71 1-Ethyl-5-{5-[(4-methyl-1,2,5-oxadiazol-3-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    72 3-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}cyclopentanone
    73 1-Ethyl-5-[5-(tetrahydro-3-furanyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    74 (4S)-4-{5[1-Ethyl4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-1,3-thiazolidin-2-one
    75 5-[5-(2,2-Dimethylcydopropyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    76 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)-N-methylacetamide
    77 1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine
    78 1-Ethyl-5-[5-(1-methylcyclobutyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    79 1-Ethyl-5-[5-(3-methyl-5-isoxazolyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    80 1-Ethyl-5-[5-(1-methyl-1H-pyrazol-5-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    81 5-[5-(1-Acetyl-4-piperidinyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    82 1-Ethyl-5-{3-[(4-methyl-1-piperazinyl)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    83 1-Ethyl-5-[3-(4-fluorophenyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    84 1-Ethyl-5-{3-[2-oxo-2-(1-pyrrolidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    85 2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-phenylacetamide
    86 2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(1-phenylethyl)acetamide
    87 1-Ethyl-5-{3-[2-oxo-2-(1-piperidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    88 2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(phenylmethyl)acetamide
    89 2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl)-N,N-dimethylacetamide
    90 N-Ethyl-2-{5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl} acetamide
    92 1-Ethyl-5-{3-[1-(4-morpholinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    93 5-[3-(Cyclohexylmethyl)-1,2,4-oxadiazol-5-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    95 1-Ethyl-5-{3-[2-oxo-2-(1-piperidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    96 1-ethyl-5-{3-[2-(4-methyl-1-piperazinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    97 1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(1H-1,2,3-triazol-1-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine
    98 5-{5-[(2,4-Dimethyl-1,3-thiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine formate
    99 1-Ethyl-5-[5-(2-furanylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine formate
    100 1-Ethyl-5-[5-(3-isoxazolylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine formate
    103 1-ethyl-5-(5-{[4-(methyloxy)phenyl]methyl}-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine trifluoroacetate
    104 1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(1H-tetrazol-1-ylmethyl)- 1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine formate
    105 1-Ethyl-5-[5-(5-isothiazolylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    106 1-Ethyl-5-{5-[(3-methyl-5-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine formate
    107 5-(5-{[4-(Dimethylamino)phenyl]methyl}-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (1:1)
    108 1-Ethyl-5-{5-[(2-methyl-1,3-thiazol-4-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine formate
    109 2-[1-({5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)cyclopentyl]-N-methylacetamide trifluoroacetate
    111 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)cyclopropanecarboxamide
    112 1-Ethyl-5-{5-[(5-methyl-3-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine formate
    113 1-Ethyl-5-{5-[(5-methyl-3-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    114 1-Ethyl-5-{5-[2-(4-methyl-1,3-thiazol-5-yl)ethyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine formate
    117 5-{5-[(3,5-Dimethyl-4-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine trifluoroacetate
    118 N-(1-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}ethyl)acetamide
    119 5-{5-[(1-acetyl-4-pipeeridinyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine trifluoroacetate
    120 1-Ethyl-5-{5-[(4-methylphenyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyidin-4-amine
    121 1-Ethyl-5-[5-(4-methylphenyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H pyrazolo[3,4-b]pyridin-4-amine
    122 5-[5-(3,4-Dimethylphenyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    123 5-[5-(2,4-Dimethylphenyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    125 5-{5-[(4-Bromophenyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    126 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(phenylmethyl)-1,3-oxazole-4-carboxamide
    127 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{[4-(methyloxy)phenyl]methyl} -1,3-oxazole-4-carboxamide
    128 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(2-methylphenyl)methyl]-1,3-oxazole-4-carboxamide
    129 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(4-methylphenyl)methyl]-1,3-oxazole-4-carboxamide
    130 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(3-methylphenyl)methyl]-1,3-oxazole-4-carboxamide
    131 N-[(4-Chlorophenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide
    132 N-[(2,3-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide
    133 N-[(3,5-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide
    134 N-[(3,4-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide
    135 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(1-phenylethyl)-1,3-oxazole-4-carboxamide
    136 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{(1R)-1-[4-(methyloxy)phenyl]ethyl}-1,3-oxazole-4-carboxamide
    137 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(1R)-1-phenylpropyl]-1,3-oxazole-4-carboxamide
    138 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(4-methylphenyl)-1,3-oxazole-4-carboxamide
    139 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-({4-[(methylsulfonyl)amino]phenyl} methyl)-1,3-oxazole-4-carboxamide
    140 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{[4-(methylsulfonyl)phenyl]methyl)-1,3-oxazole-4-carboxamide
    141 N-(1-Acetyl-4-piperidinyl)-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide
    142 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1,3-oxazole-4-carboxamide
    143 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(tetrahydro-2-furanylmethyl)-1,3-oxazole-4-carboxamide
    144 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[2-(4-methyl-1-piperazinyl)ethyl]-1,3-oxazole-4-carboxamide
    145 N-[1-(Aminomethyl)cyclohexyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-methyl-1,3-oxazole-4-carboxamide
    146 N-(2,6-Dimethylphenyl)-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide
    147 N-{[4-(Aminocarbonyl)phenyl]methyl}-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide
    148 2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(tetrahydro-2H-pyran-4-yl)acetamide
    149 5-{3-[2-(2,6-Dimethyl-4-morpholinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    150 1-Ethyl-5-{3-[2-(4-methyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    152 2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-[1-methyl-2-(methyloxy)ethyl]acetamide
    153 5-{3-[2-(3,5-Dimethyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    154 1-Ethyl-5-{3-[2-(3-methyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    155 2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pytidin-5-yl]-1,2,4-oxadiazol-3-yl}-N-3-pyridinylacetamide
    157 6-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-2-piperidinone
    158 1-Ethyl-5-{5-[(3-methyl-1H-1,2,4-triazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    159 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)acetamide
    160 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)benzamide
    161 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-phenylacetamide
    162 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-methylpropanamide
    163 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-3-methylbutanamide
    164 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)cyclohexanecarboxamide
    165 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-furancarboxamide
    166 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)methanesulfonamide
    167 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)benzenesulfonamide
    168 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-1-phenylmethanesulfonamide
    169 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-propanesulfonamide
    170 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-1-propanesulfonamide
    171 N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)cyclopropanesulfonamide
    172 N-({5-[1-Ethyl-4-(tetrahydro-2H-pxan-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-thiophenesulfonamide
    173 1-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-pyrrolidinone
    174 1-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-piperidinone
    175 5-{3-[(1-Acetyl-4-piperidinyl)methyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    176 1-Ethyl-5-(3-{[1-(3-methylbutanoyl)-4-piperidinyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    177 1-Ethyl-5-(3-{[1-(methylsulfonyl)-4-piperidinyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    178 1-Ethyl-5-{3-[1-(phenylsulfonyl)cyclopropyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    179 1-Ethyl-5-[3-(phenylmethyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    180 1-Ethyl-5-[3-(1-phenylethyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    181 1-Ethyl-5-(3-{[4-(methyloxy)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    182 5-(3-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    183 5-(3-{[3-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    184 5-(3-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tedahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    185 1-Ethyl-5-{3-[(phenyloxy)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    186 1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[3-(5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyridin-3-ylmethyl)-1,2,4-oxadiazol-5-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine
    187 1-Ethyl-5-{3-[(4-phenyl-1-piperazinyl)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    188 1-Ethyl-5-(5-ethyl-1,2,4-oxadiazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    189 5-(5-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-3-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    190 1-Ethyl-5-(5-{[4-(methyloxy)phenyl]methyl}-1,2,4-oxadiazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine
    191 5-(3,8-Dioxa-1-azaspiro[4.5]dec-1-en-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine

    Example 1: N-Cyclopentyl-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0549] 



    [0550] Intermediate 4 (0.043g) was dissolved in acetonitrile (2ml) then treated with phosphorous oxychloride (0.101g) and stirred under nitrogen and heated at 90°C for 2h. The mixture was concentrated in vacuo and the residue partitioned between DCM and saturated aqueous sodium bicarbonate solution. The organic layer was concentrated in vacuo and applied to an SPE cartridge (aminopropyl, 1g), which was eluted with methanol. Concentration in vacuo afforded Example 1 (0.032g). LCMS showed MH+ = 313; TREY = 3.13min.

    [0551] Similarly prepared, for example without limitation using the same or similar number of moles of reagents and/or volumes of solvents, but with an extended reaction time (see table) was:
     RYStarting materialReaction timeMH+ ionTRET(min)
    Example 2

    Intermediate 5 3h 391 2.88

    Example 3: N-Cyclopentyl-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0552] 



    [0553] Intermediate 10 was dissolved in acetonitrile (2ml) then treated with phosphorous oxychloride (0.101g) and stirred under nitrogen at 90°C for 3.5h. The mixture was concentrated in vacuo and the residue partitioned between DCM and saturated aqueous sodium bicarbonate solution. The organic layer was concentrated in vacuo and the residue applied to a SPE cartridge (silica, 5g), which was eluted with cyclohexane : Et2O (1:2). Fractions containing desired material were combined and concentrated in vacuo to afford Example 3 (0.034g). LCMS showed MH+ = 341; TRET = 3.39min.

    Example 4: N-Cyclopentyl-1-ethyl-5-(5-methyl-1,3,4-thiadinzol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0554] 



    [0555] A solution of Intermediate 4 (0.09g) in acetonitrile (5ml) was stirred under nitrogen and treated with Lawesson's reagent (0.116g). The mixture was heated at 65°C for 16h, then concentrated in vacuo. The residue was applied to an SPE cartridge (silica, 5g) and eluted with a gradient of cyclohexane : Et2O (1:2 then 1:3, 1:4, 1:5, 0:1). Fractions containing desired material were combined and concentrated in vacuo. Further purification was achieved using mass directed autoprep HPLC to afford Example 4 (0.002g). LCMS showed MH+ = 339; TRET = 3.23min.

    Example 5: N-Cyclopentyl-1-ethyl-5-(5-[(methylsulfonyl)methyl]-1,3,4-thiadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0556] 



    [0557] A solution of Intermediate 5 (0.07g) in acetonitrile (3ml) was stirred under nitrogen and treated with Lawesson's reagent (0.085g). The mixture was heated at 65°C for 136h, then concentrated in vacuo. The residue was partitioned between DCM and water and the organic layer concentrated in vacuo. Further purification was achieved using mass directed autoprep HPLC to afford Example 5 (0.008g). LCMS showed MH+ = 407; TRET = 2.98min.

    Example 6: N-Cyclopentyl-1-ethyl-5-(5-isopropyl-1,3,4-thiadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0558] 



    [0559] Intermediate 10 was dissolved in acetonitrile (5ml) then treated with Lawesson's reagent (0.125g) and heated under nitrogen at 65C for 66h. Volatiles were removed in vacuo and the residue was purified by mass directed autoprep HPLC to afford Examples 6. LCMS showed MH+ = 357; TRET = 3.59min.

    Reference Example 7: 1-Ethyl-N-(4-fluorophenyl)-5-(3-methyl-1,2,4-oxadiazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0560] 



    [0561] A solution of Intermediate 6 (0.04g) in ethanol (1ml) was stirred over powdered 4A molecular sieves (0.290g) and treated with Intermediate 9 (0.045g), followed by sodium ethoxide (0.020g). The mixture was heated under reflux for 18h, then cooled and filtered. Following concentration of the filtrate in vacuo, the residue was applied to an SPE cartridge (silica, 5g) which was eluted with cyclohexane : Et2O (1:1). Fractions containing desired material were combined and concentrated in vacuo to afford Reference Example 7 (0.017g). LCMS showed MH+ = 339; TRET = 3.23min.

    Reference Example 8: N-Cyclopentyl-5-(1,3-dimethyl-1H-1,2,4-triazol-5-yl)-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0562] 



    [0563] A solution of Intermediate 7 (0.06g) in ethanol (2ml) was treated with triethylamine (0.101g), followed by methyl acetimidate hydrochloride (0.033 g) and the mixture heated under reflux (80°C) for 42h. Reaction was incomplete so a further portion of methyl acetimidate hydrochloride (0.033g) was added and stirring continued under reflux for 6 days. The mixture was concentrated in vacuo and the residue partitioned between DCM and 2M aqueous HCl. The organic layer was concentrated in vacuo and purified by mass directed autoprep to afford Reference Example 8 (0.003g). LCMS showed MH+ = 326; TRET = 2.66min.

    Example 9: 1-Ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0564] 

    Intermediate 13 (0.016g) was dissolved in anhydrous acetonitrile (1ml).
    4-Aminotetrahydropyran hydrochloride (Intermediate 21A, 0.008g) was then added, followed by diisopropylethyl amine (0.05ml) and the mixture was stirred under nitrogen at 75°C for 19h. A further portion of 4-aminotetrahydropyran (0.002g) was added and stirring continued at 85°C for 16h. The mixture was concentrated in vacuo and partitioned between DCM and water. The organic phase was concentrated in vacuo and applied to an SPE cartridge (silica, 1g), which was eluted sequentially with a gradient of EtOAc: cyclohexane (i) 1:8, (ii) 1:4, (iii) 1:2, (iv) 1:1 and (v) 1:0. Fractions containing desired material were combined and concentrated in vacuo to afford Example 9 (0.013g). LCMS showed MH+ = 357; TRET = 2.89min.

    Example 10: N-cyclohesyl-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0565] 



    [0566] Intermediate 13 (0.016g, 0.055 mmol) was dissolved in anhydrous acetonitrile (1ml). Cyclohexyl amine (0.007ml, 0.061 mmol) was then added, followed by diisopropylethyl amine (0.05ml), 0.29 mmol) and the mixture was stirred under nitrogen at 75°C for 16h. The mixture was concentrated in vacuo and partitioned between DCM and water. The organic phase was concentrated in vacuo and applied to an SPE cartridge (silica, 1g), which was eluted sequentially with a gradient of EtOAc: cyclohexane (i) 1:16, (ii) 1:8, (iii) 1:4, (iv) 1:2 and (v) 1:1. Fractions containing desired material were combined and concentrated in vacuo to afford Example 10 (0.015g). LCMS showed MH+ = 355; TRET = 3.59min.

    [0567] Similarly prepared using the same or similar number of moles of reagents and volumes of solvents was the following:
     NR3R3aStarting amineMH+ ionTRET(min)
    Reference Example 11

    Isobutyl amine 329 3.40

    Reference Example 12: 1-Ethyl-N-isobutyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0568] 



    [0569] Intermediate 12 (0.026g, 0.1 mmol) was dissolved in ethanol (1.5ml) and treated with a solution of isobutylamine (0.007g, 0.1 mmol), also in ethanol (lml). The mixture was then treated with diisopropylethyl amine (0.075 ml, 0.4 mmol, 4 mole equivalents) and stirred at 75°C for 16h. The mixture was concentrated in vacuo and applied to an SPE cartridge (silica, 0.5g) which was eluted sequentially with (i) chloroform, (ii) Et2O and (iii) methanol. Fractions containing desired material were combined and concentrated in vacuo to afford Reference Example 12 (0.024g). LCMS showed MH+ = 301; TRET = 2.90min.

    [0570] Similarly prepared using the same or similar number of moles of reagents and volumes of solvents were the following:
     RYNR3R3aStarting materialAmine reagentMH+ ionTRET (min)
    Example 13 Me

    Intermediate 12 Cyclohexylamine ion 327 3.12
    Example 14 Me

    Intermediate 12 4-Amino tetrahydropyran 329 2.49
    Reference Example 15 Me

    Intermediate 12 (R)-(-)-3-methyl-2-butylamine 315 3.00
    Reference Example 16 Me

    Intermediate 12 (S)-(-)-3-methyl-2-butylamine 315 3.00
    Example 17 tBu

    Intermediate 14 4-Amino tetrahydropyran 371 2.99
    Example 18 tBu

    Intermediate 14 Cyclohexylamine 369 3.64
    Example 19 tBu

    Intermediate 14 Cyclopentylamine 355 3.48
    Reference Example 20 tBu

    Intermediate 14 Isobutylamine 343 3.43
    Reference Example 21 tBu

    Intermediate 14 (S)-(-)-3-methyl-2-butylamine 357 3.53
    Reference Example 22 tBu

    Intermediate 14 (R)-(-)-3-methyl-2-butylamine 357 3.53
    Example 23



    Intermediate 15 4-Amino tetrahydropyran 407 2.44
    Example 24



    Intermediate 15 Cyclohexylamine 405 3.00
    Reference Example 25



    Intermediate 15 Isobutylamine 379 2.81
    Reference Example 26



    Intermediate 15 (S)-(-)-3-methyl-2-butylamine 393 2.90
    Reference Example 27



    Intermediate 15 (R)-(-)-3-methyl-2-butylamine 393 2.91

    Example 14: 1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0571] 



    [0572] An alternative method of preparing Example 14 is now described:

    EDC (0.823g, 5.3mmol) and HOBT (0.614g, 4.55mmol) were added to Intermediate 17 (1.10g, 3.80mmol)) in N,N'-dimethylformaraide (10ml). The mixture was stirred for 1.5 hours then acetic hydrazide (0.421g, 5.7mmol) (commercially available e.g. from Aldrich) was added and the reaction mixture stirred at 20 °C for 48 hours. The reaction mixture was evaporated and the residue partitioned between chloroform and water. The aqueous phase was extracted with chloroform and the combined organic phases were washed with saturated aqueous sodium chloride solution then dried (Na2SO4) and evaporated. Phosphorus oxychloride (10ml) was added to the residue and the mixture heated at 120 °C for 0.5 hours. The reaction mixture was evaporated in vacuo and the residue applied to an SPE cartridge (silica, 20g). The cartridge was eluted with dichloromethane, cyclohexane:ethyl acetate (2:1 then 1:1), ethyl acetate, chloroform:methanol (19:1 followed by 9:1). Fractions containing the required compounds were combined and evaporated in vacuo. The residue was then chromatographed on the Biotage (silica, 50g) using cyclohexane:ethyl acetate (2:1 then 1:1), ethyl acetate followed by ethyl acetate:ethanol (19:1, 9:1 then 9:2). The residue was partitioned between dichloromethane and aqueous sodium hydrogencarbonate solution. The organic phase was dried (Na2SO4) and evaporated in vacuo to give Example 14 as a pale yellow solid (0.93g). LCMS showed MH+ = 329, TRET = 2.54min. 1H NMR (400MHz in CDCl3, 27°C, δppm) 9.12 (br m, 1H), 8.72 (s, 1H), 8.01 (s, 1H), 4.52 (q, 2H), 4.24 (m, 1H), 4.08 (m, 2H), 3.67 (m, 2H), 2.65 (s, 3H), 2.20 (m, 2H), 1.36 (m, 2H), 1.53 (t, 3H).


    Example 17: 5-[5-(1,1-Dimethylethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0573] 



    [0574] An alternative method of preparing Example 17 is now described:

    EDC (1.30g, 6.76mmol) and HOBT (0.782g, 5.80mmol) were added to Intermediate 17 (1.40g, 4.83mmol) in N,N'-dimethylformamide (20ml). The mixture was stirred for 0.5 hours then pivalic acid hydrazide (0.616g, 5.3mmol) (commercially available from Fluorochem Ltd, Wesley Stree, Glossop, Derbyshire SK13 9RY, United Kingdom or can be prepared according to the procedure by K. Ohmoto et al. in J. Med. Chem., 2001, 44(8), 1268) was added and the reaction mixture stirred at 20 °C for 18 hours. The reaction mixture was evaporated and the residue partitioned between dichloromethane and water. The organic phase was washed with water, saturated aqueous sodium hydrogen carbonate solution followed by saturated aqueous sodium chloride solution then evaporated in vacuo. Phosphorus oxychloride (10ml) was added to the residue and the mixture heated at 120 °C for 3 hours. The reaction mixture was evaporated in vacuo and the residue partitioned between dichloromethane and water. The organic phase was washed with aqueous sodium hydrogen carbonate solution then dried and evaporated in vacuo. The residue was applied to an SPE cartridge and eluted with cyclohexane: ethyl acetate (3:1 followed by 7:3). The solvent was evaporated in vacuo to give Example 17 as a white solid (0.65g). LCMS showed N4H+ = 371, TRET = 3.05min. 1H NMR (400MHz in CDCl3, 27°C, δppm) 9.18 (br m, 1H), 8.75 (s, 1H), 8.01 (s, 1H), 4.52 (q, 2H), 4.25 (m, 1H), 4.08 (m, 2H), 3.67 (m, 2H), 2.20 (m, 2H), 1.84 (m, 2H), 1.57-1.49 (m, 12H).


    Example 28: 1-Ethyl-5-(3-methyl-1,2,4-oxadiazol-5-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0575] 



    [0576] A solution of Intermediate 16 (0.05g, 0.157 mmol) in ethanol (2ml) was stirred over powdered 4Å molecular sieves (0.30g) and treated with a solution of Intermediate 9 (0.059g, 0.8 mmol) and sodium ethoxide (0.027g, 0.4 mmol) in ethanol (1ml). The mixture was heated at reflux for 18h under nitrogen, then cooled and filtered. Following concentration of the filtrate in vacuo, the residue was applied to an SPE cartridge (silica, 5g) which was eluted with cyclohexane: EtOAc (1:1). Fractions containing desired material were combined and concentrated in vacuo to afford Example 28 (0.024g). LCMS showed MH+ = 329; TRET = 2.86 min.

    [0577] Similarly prepared using the same or similar number of moles of reagents and volumes of solvents were the following:
     RXStarting AmidoximeMH+ ionTRET (min)
    Example 29 CH2OMe Intermediate 22 359 2.78

    Example 30: 5-{3-[(Dimethylamino)methyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0578] 



    [0579] A solution of Intermediate 16 (0.05g) in ethanol (2ml) was stirred over powdered 4Å molecular sieves (0.30g) and treated with a solution of Intermediate 23 (0.094g) and sodium ethoxide (0.027g) in ethanol (1ml). The mixture was heated at reflux for 18h under nitrogen, then cooled and filtered. Following concentration of the filtrate in vacuo, the residue was applied to an SPE cartridge (silica, 5g) which was eluted with 2-5% methanol in DCM. Fractions containing desired material were combined and concentrated in vacuo, then applied to a further SPE cartridge (aminopropyl, 1g) which was eluted with methanol to afford Example 30 (0.02g). LCMS showed MH+ = 372; TRET = 2.10 min.

    Example 31: 1-Ethyl-5-[3-(morpholin-4-ylmethyl)-1,2,4-oxadiazol-5-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0580] 



    [0581] A solution of Intermediate 16 (0.05g) in ethanol (2ml) was stirred over powdered 4Å molecular sieves (0.30g) and treated with a solution of Intermediate 24 (0.128g) and sodium ethoxide (0.027g) in ethanol (1ml). The mixture was heated at reflux for 18h under nitrogen, then cooled and filtered. Following concentration of the filtrate in vacuo, the residue was applied to an SPE cartridge (silica, 5g) which was eluted with 2-5% methanol in DCM. Fractions containing desired material were combined and concentrated in vacuo to afford Example 31 (0.025g). LCMS showed MH+ = 415; TRET = 2.46 min.

    Example 32: 5-(5-Cyclopropyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0582] 



    [0583] A solution of Intermediate 20 (0.020g) in THF (0.2ml) was treated with Burgess reagent (0.026g) and heated in a microwave at 120°C (100W) for 5min. The mixture was concentrated by evaporation under a stream of nitrogen and the residue applied to an SPE cartridge (silica, 1g) which was eluted with 2% methanol in DCM to afford Example 32 as a white solid (0.014g). LCMS showed MH+ = 355; TRET = 2.78 min.

    Example 33: N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo [3,4-b]pyridin-4-amine



    [0584] 



    [0585] Intermediate 12 (0.03g) was dissolved in acetonitrile (2ml) and treated with DIPEA (0.1ml) and Intermediate 25 (0.022g). The mixture was stirred at 85°C for 18h then concentrated in vacuo and partitioned between DCM and water. The layers were separated and the organic layer concentrated in vacuo, then purified by mass directed autoprep HPLC to afford Example 33 (0.01g). LCMS showed MH+ = 370; TRET = 2.48min.

    Example 34: 1-Ethyl-5-[5-(3-methyloxetan-3-yl)-1,3,4-oxadiazol-2-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0586] 



    [0587] A solution of Intermediate 19 (0.05g, 0.133 mmol), TBTU (0.045g, 0.14 mmol) and DIPEA (0.1ml), ca. 0.5 mmol) in DMF (1ml) is stirred at room temperature under nitrogen for 5 min. A solution of Intermediate 26 (0.024g, 0.21 mmol) in DMF (1ml) is then added and stirring continued for 18h. Reaction can be found to be incomplete after this time so a further portion of Intermediate 26 (0.412g, 0.10 mmol) is added and stirring continued under nitrogen for a further 18h. The mixture is concentrated in vacuo then the residue applied to an SPE cartridge (aminopropyl, 2g), which is eluted with methanol (2x3ml). Fractions containing desired material are concentrated in vacuo.

    [0588] The partially purified intermediate is taken forward without further characterisation and si dissolved in THF (0.5ml) then treated with Burgess reagent (0.025g, ca. 0.1 mmol). The mixture is heated under microwave conditions at 120°C (120W) for 5 min. The mixture is then concentrated in vacuo and purified by mass directed autoprep HPLC to afford Example 34.

    [0589] According to an alternative and more preferred embodiment, the reaction was performed as follows. A solution of carboxylic acid Intermediate 26 (0.024g, 0.21 mmol), TBTU (0.045g, 0.14 mmol) and DIPEA (0.1ml, ca. 0.5 mmol) in DMF (1ml) was stirred at room temperature under nitrogen for 5 min. A solution of Intermediate 19 (0.05g, 0.133 mmol) in DMF (1ml) was then added and stirring continued for 18h. Reaction was found to be incomplete after this time so a further portion of Intermediate 26 (0.012g, 0.10 mmol) was added and stirring continued under nitrogen for a further 18h. The mixture was concentrated in vacuo then the residue applied to an SPE cartridge (aminopropyl, 2g), which was eluted with methanol (2x3ml). Fractions containing desired material were concentrated in vacuo. The partially purified intermediate was taken forward without further characterisation and was dissolved in THF (0.5ml) then treated with Burgess reagent (0.025g, ca. 0.1 mmol). The mixture was heated under microwave conditions at 120°C (120W) for 5 min. The mixture was then concentrated in vacuo and purified by mass directed autoprep HPLC to afford Example 34 (0.006g). LCMS showed MH+ = 385; TRET = 2.65min.

    [0590] Similarly prepared, via the original or alternative embodiment described above, and using the same or similar number of moles of reagents and volumes of solvents, were the following:
     RYStarting Carboxylic Acid (instead of Intermediate 26)MH+ ionTRET (min)
    Example 35

    Intermediate 27 427 2.14
    Example 36

    Intermediate 28 400 2.87
    Example 37

    Intermediate 29 412 2.39

    Example 35: 1-Ethyl-5-{5-[(4-methyl-1-piperazinyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0591] 



    [0592] An alternative method of preparing Example 35 is now described:

    A solution of Intermediate 27 (0.463g, 2.93mmol), TBTU (0.941g, 2.93mmol) and DIPEA (1.53ml, 8.79mmol) in dry dimethylformamide (7ml) was stirred at room temperature for 15min. A solution of Intermediate 19 (1.0g, 2.93mmol) in dry dimethylformamide (5ml) was then added and stirring was continued for 1h. The mixture was concentrated in vacuo, and the residue was dissolved in methanol (5ml) and applied equally to two SPE cartridges (aminopropyl, 10g). The cartridges were eluted with methanol. The product-containing fractions were combined and evaporated to give a yellow oil (1.56g) which was dissolved in dichloromethane (10ml) and applied to a SPE cartridge (silica, 10g). The cartridge was eluted with chloroform-methanol-triethylamine (9/0.2/0.1). Fractions containing the desired product were combined and evaporated to give a pale yellow foam (1.17g). This product was suspended in dry tetrahydrofuran (45ml) and treated with Burgess reagent (1.244g, 5.22mmol) at room temperature under nitrogen. The resulting solution was heated at 70°C. After 2h, the reaction mixture was evaporated and the residual oil was dissolved in dichloromethane (5ml) and applied to a SPE cartridge (silica, 20g). The cartridge was eluted with chloroform-methanol-triethylamine (9/0.2/0.1). Fractions containing the desired material were combined and evaporated to give a cream solid. Further purification by passage through a SCX cartridge (20g) eluting with methanol followed by 10% ammonia in methanol afforded Example 35 as a buff coloured solid (0.72g). LCMS showed MH+ = 427, TRET = 2.02min. 1H NMR (400MHz in CDCl3, 27°C, δppm) 9.11 (d, 7Hz, 1H), 8.76 (s, 1H), 8.01 (s, 1H), 4.52 (q, 2H), 4.24 (m, 1H), 4.08 (m, 2H), 3.93 (s, 2H) 3.66 (m, 2H), 2.8 - 2.5 (br m's, 4H), 2.31 (s, 3H), 2.20 (m, 2H), 1.85 (m, 2H), 1.52 (t, 3H).


    Example 38: 1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0593] 



    [0594] A solution of Intermediate 19 (0.05g, 0.133 mmol), TBTU (0.045g, 0.14 mmol) and DIPEA (0. 1ml, ca. 0.5 mmol) in DMF (1ml) is stirred at room temperature under nitrogen for 5 min. A solution of Intermediate 30 (0.018g, 0.14 mmol) in DMF (1ml) is then added and stirring continued for 18h. Reaction can be found to be incomplete after this time so a further portion of Intermediate 30 (0.009g, 0.07 mmol) is added and stirring continued under nitrogen for a further 18h. The mixture is concentrated in vacuo then the residue applied to an SPE cartridge (aminopropyl, 2g), which is eluted with methanol (2x3ml). Fractions containing desired material are concentrated in vacuo. The partially purified intermediate is taken forward without further characterisation and is dissolved in THF (0.5ml) then treated with Burgess reagent (0.025g, ca. 0.1 mmol). The mixture is heated under microwave conditions at 120°C (120W) for 5 min. Reaction can appear incomplete so a further portion of Burgess Reagent (0.012g, ca. 0.05 mmol) is added and the mixture heated under microwave conditions at 140°C (120W) for a further 10 min. The mixture is then concentrated in vacuo and purified by mass directed autoprep HPLC to afford Example 38.

    [0595] According to an alternative and more preferred embodiment, the reaction was performed as follows. A solution of carboxylic acid Intermediate 30 (0.018g, 0.14 mmol), TBTU (0.045g, 0.14 mmol) and DIPEA (0.1ml, ca. 0.5 mmol) in DMF (1ml) was stirred at room temperature under nitrogen for 5 min. A solution of Intermediate 19 (0.05g, 0.133 mmol) in DMF (1ml) was then added and stirring continued for 18h. Reaction was found to be incomplete after this time so a further portion of Intermediate 30 (0.009g, 0.07 mmol) was added and stirring continued under nitrogen for a further 18h. The mixture was concentrated in vacuo then the residue applied to an SPE cartridge (aminopropyl, 2g), which was eluted with methanol (2x3ml). Fractions containing desired material were concentrated in vacuo. The partially purified intermediate was taken forward without further characterisation and was dissolved in THF (0.5ml) then treated with Burgess reagent (0.025g, ca. 0.1 mmol). The mixture was heated under microwave conditions at 120°C (120W) for 5 min. Reaction appeared incomplete so a further portion of Burgess Reagent (0.012g, ca. 0.05 mmol) is added and the mixture heated under microwave conditions at 140°C (120W) for a further 10 min. The mixture was then concentrated in vacuo and purified by mass directed autoprep HPLC to afford Example 38 (0.006g). LCMS showed MH+ = 399; TRET = 2.64min.

    [0596] Similarly prepared, via the original or alternative embodiment described above, and using the same or similar number of moles of reagents and volumes of solvents, were the following:
     RYStarting Carboxylic Acid (instead of Intermediate 30)MH+ ionTRET (min)
    Example 39

    Intermediate 31 414 2.44
    Example 40 CH2OtBu Intermediate 32 401 2.98

    Example 38: 1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0597] 



    [0598] An alternative method of preparing Example 38 is now described:

    A mixture of Intermediate 30 (0.325g, 2.5mmol), TBTU (0.803g, 2.5mmol) and DIPEA (1.75ml, 10.04mmol) in N,N-dimethylformamide (10ml) was stirred at 20 °C for 20 minutes. A suspension of Intermediate 19 (1.024g, 3.00mmol) in N,N-dimethylformamide was added and the reaction mixture stirred for 18 hours. The solvent was evaporated and the residue applied to SPE cartridges (2 x 50g, aminopropyl). The cartridges were eluted with dichloromethane:methanol (0 - 100% methanol over 17 minutes at 25ml/min). Appropriate fractions were evaporated in vacuo and the residue dissolved in tetrahydrofuran (10ml). Burgess Reagent (0.746g, 3.13mmol) was added and the reaction mixture was heated at reflux for 2.5 hours. Additional Burgess Reagent (0.284g) was added and heating continued for 1.5 hours. The solvent was evaporated in vacuo. The residue was applied to an SPE cartridge (silica, 100g) and eluted with cyclohexane:ethyl acteate (gradient of 0 to 100% ethyl acetate over 25 minutes at 25ml/min) followed by ethyl acetate then ethyl acetate:methanol (4:1). Appropriate fractions were combined and evaporated to give Example 38 as a white solid (0.503g). LCMS showed MH+ = 399, TRET = 2.67min. 1H NMR (400MHz in CDCl3, 27°C, δppm) 9.14 (br m, 1H), 8.72 (s, 1H), 8.01 (s, 1H), 4.52 (q, 2H), 4.24 (m, 2H), 4.10 (m, 4H), 3.64 (m, 4H), 3.27 (m, 1H), 2.25-1.96 (m, 6H), 1.85 (m, 2H), 1.53 (t, 3H).


    Example 40A: Methyl 2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-4,5-dihydro-1,3-oxazole-4-carboxylate



    [0599] 



    [0600] Intermediate 33 (0.055g, 0.14mmol) and Burgess reagent (0.037g, 0.16mmol) were suspended in THF (2ml) and heated at reflux for 4 hours. Solvents were removed in vacuo and the residue applied to an SPE cartridge (silica, 2g), which was eluted with cyclohexane:ethyl acetate (1:2). Concentration in vacuo afforded Example 40A (0.03 g). LCMS showed MH+ = 374, TRET = 2.78min.

    Example 41: Methyl 2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxylate



    [0601] 



    [0602] The compound of Example 41 was synthesised using the following route, reagents and solvents:



    [0603] In one embodiment, a suitable detailed procedure for the first two steps is given above in "Intermediate 33" and "Example 40A". In one embodiment, a suitable detailed procedure for synthesising Example 41 from Example 40A is as follows:

    [0604] Example 40A (0.023g, 0.062mmol) and DBU (0.028g, 0.18mmol) were dissolved in carbon tetrachloride/acetonitrile/pyridine (2:3:3, 1.6ml) and stirred at room temperature under nitrogen for 48 hours. Solvents were removed in vacuo and the residue was purified by mass directed autoprep HPLC to afford Example 41 (0.0017g). LCMS showed MH+ = 372, TRET = 9.24min.

    Example 42: 1-Ethyl-5-(4-methyl-4,5-dihydro-1,3-oxazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0605] 



    [0606] Intermediate 34 (0.095g, 0.27mmol) and Burgess reagent (0.071 g, 0.30mmol) were dissolved in THF (2ml) and heated at reflux for 4 hours. Solvents were removed in vacuo and the residue applied to an SPE (silica, 5g), which was eluted with ethyl acetate to afford Example 42 (0.045g). LCMS showed MH+ = 330, TRET= 2.84min.

    Example 43: 1-n-Propyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0607] 


    Example 43 was synthesised according to the following reaction scheme:



    [0608] 



    [0609] Detailed conditions which can be used for the first six reactions from Intermediate 1 to Intermediate 40 are given in the "Intermediate" syntheses hereinabove for Intermediates 35, 36, 37, 38, 39 and 40.

    [0610] Example 43 can be made from Intermediate 40 using a similar process to that described for Example 1, 2, 3, using a similar or the same number of moles of reagents and/or volumes of solvents. LCMS showed MH+ = 343, TRET = 2.70min.

    Example 44: 1-Ethyl-5-[5-(tetrahydrofuran-2-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0611] 



    [0612] A solution of Tetrahydro-2-furoic acid = 2-(tetrahydrofuran)carboxylic acid (commercially available from Sigma-Aldrich) (0.012ml, 0.12mmol), TBTU (0.039g, 0.12mmol) and DIPEA (0.084ml, 0.48mmol) in DMF (2ml) was stirred at room temperature under nitrogen. Intermediate 19 (0.045g, 0.12mmol) was added and the reaction stirred for 2 days. The mixture was concentrated in vacuo then the residue applied to an SPE cartridge (aminopropyl, 5g), which was eluted with methanol. Fractions containing the desired material were concentrated in vacuo. Half of the partially purified material was dissolved in THF (0.1ml) and treated with Burgess reagent (0.015g, 0.06mmol). The mixture was heated under microwave conditions at 120 °C (100W) for 5 minutes. The mixture was then concentrated in vacuo and applied to an SPE cartridge (silica, 0.5g). The cartridge was eluted with dichloromethane: methanol (19:1), fractions containing the desired material were concentrated in vacuo. The sample was then partitioned between dichloromethane and water, the organic phase was evaporated to give Example 44 (0.0065g). LCMS showed MH+ =385, TRET = 2.69min.

    Example 45: 1-Ethyl-5-[5-(dimethylamino)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0613] 



    [0614] Intermediate 86 (0.113g, 0.28mmol) and Burgess Reagent (0.133g, 0.56mmol) in THF (1ml) were heated in the microwave 5 minutes at 120 °C SmithCreator Microwave. The sample was evaporated in vacuo and the residue purified by mass directed autoprep HPLC...LCMS showed MH+ = 358; TRET = 2.57min

    Example 46: 1-Ethyl-5-(5-methyl-1,2,4-triazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0615] 



    [0616] A solution of the Intermediate 19 (0.1g, 0.29mmol), diisopropylethylamine (0.3ml, 1.74mmol) and methyl acetimidate hydrochloride (0.095g, 0.87mmol, commercially available from Aldrich) in ethanol (3ml) was heated under reflux. After 17h, the reaction mixture was evaporated to an oily residue which was partitioned between dichloromethane (10ml) and water (2ml). The phases were separated and the organic phase was dried over anhydrous sodium sulphate and evaporated to a waxy solid (0.053g). Purification of a portion of this solid (0.025g) by mass directed autoprep HPLC afforded Example 46 (0.005g). LCMS showed MH+ = 328; TRET = 2.25min.

    Example 47: N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-(3-methyl-1,2,4-oxadiazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0617] 



    [0618] A mixture of Intermediate 41 (0.049mg, 0.14mmol), Intermediate 9 (0.051g, 0.68mmol), sodium ethoxide (0.13m, 21% solution in ethanol, commercially available from Aldrich) and powdered 4Å molecular sieves (0.3g) in ethanol (2ml) were heated at 80 °C for 16 hours under nitrogen. The mixture was cooled and filtered and the filtrate concentrated in vacuo. The residue was applied to an SPE cartridge (silica, 5g) and eluted with cyclohexane, cyclohexane:ethyl acetate (1:1) and then ethyl acetate. The desired fractions were combined and evaporated to give Example 47 (0.005g). LCMS showed MH+ = 370; TRET = 2.77min

    Example 48: N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-[3-(morpholin-4-ylmethyl)-1,2,4-oxadiazol-5-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0619] 



    [0620] Example 48 was prepared from Intermediate 41 and Intermediate 24 using an analogous method to that for Example 47. LCMS showed MH+ = 455; TRET = 2.59min.

    Example 49 1-Ethyl-5-[(4R)-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0621] 



    [0622] Burgess reagent (0.189g, 0.79mmol) was added portionwise, over 3min, to a stirred solution of Intermediate 42 (0.293g, 0.72mmol) in dry tetrahydrofuran (13ml) at room temperature under nitrogen. The resulting solution was heated at 70°C under nitrogen for 4h. The reaction mixture was evaporated to give an off-white solid which was dissolved in dichloromethane (5ml) and applied to a SPE cartridge (silica, 10g). The cartridge was eluted sequentially with a gradient of ethyl acetate-petroleum ether (1:8, 1:4, 1:2, 1:1, 1:0). Fractions containing the desired product were combined and evaporated to afford Example 49 as a white crystalline solid (0.169g). LCMS showed MH+ = 392; TRET = 3.31min.

    Example 50 1-Ethyl-5-[(4S)-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0623] 



    [0624] Example 50 was prepared from Intermediate 43 using an analogous method to that for Example 49. LCMS showed MH+ = 392; TRET = 3.32min.

    Example 51 1-Ethyl-5-[(4S)-4-(phenylmethyl)-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0625] 



    [0626] Example 51 was prepared from Intermediate 44 using an analogous method to that for Example 49. LCMS showed MH+ = 406; TRET = 3.38min

    Example 52 1-Ethyl-5-[(4R)-4-(phenylmethyl)-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0627] 



    [0628] Example 52 was prepared from Intermediate 45 using an analogous method to that for Example 49. LCMS showed MH+ = 406; TRET = 3.38min.

    Example 53 1-Ethyl-5-[(4S,5R)-5-methyl-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0629] 



    [0630] Example 53 was prepared from Intermediate 46 using an analogous method to that for Example 49. LCMS showed MH+ = 406; TRET = 3.37min.

    Example 54 1-Ethyl-5-[(5R)-5-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0631] 



    [0632] Example 54 was prepared from Intermediate 47 using an analogous method to that for Example 49. LCMS showed MH+ = 392; TRET = 3.29min.

    Example 55 1-Ethyl-5-[(5S)-5-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0633] 



    [0634] Example 55 was prepared from Intermediate 48 using an analogous method to that for Example 49. LCMS showed MH+ = 392; TRET = 3.29min.

    Example 56 5-(4,4-Dimethyl-4,5-dihydro-1,3-oxazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0635] 



    [0636] Example 56 was prepared from Intermediate 49 using an analogous method to that for Example 49. LCMS showed MH+ = 344; TRET = 2.95min.

    Example 57: 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxylic acid



    [0637] 



    [0638] A solution of lithium hydroxide (0.12g, 5.2mmol) in water (6ml) was added to a suspension of Example 41 (0.48g, 1.3mnlol) in methanol (20ml) and the resultant mixture heated at 50 °C for 2 hours. The solvent was evaporated in vacuo and the residue dissolved in water (50ml), cooled in an ice bath and acidified to pH 3 by the addition of aqueous hydrochloric acid. The precipitate was filtered, washed with water and dried in vacuo at 40 °C to give Example 57 as a white solid (0.3g). LCMS showed MH+ = 358; TRET = 2.62min

    Example 58: 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(1-methylethyl)-1,3-oxazole-4-carboxamide



    [0639] 



    [0640] A mixture of Example 57 (0.05g, 0.14mmol), HOBT (0.023g, 0.17mmol), EDC (0.038g, 0.2mmol) in DMF (2ml) were stirred at 20 °C for 20 minutes. Isopropylamine (0.013ml, 0.15mmol) was added and the reaction mixture stirred overnight. The solvent was concentrated in vacuo and the residue dissolved in DCM. The organic phase was washed with water then aqueous sodium hydrogen carbonate solution. The aqueous phases were extracted with DCM and the combined organic phases concentrated in vacuo. The residue was applied to an SPE cartridge (aminopropyl, 2g) and eluted with MeOH, appropriate fractions were combined and evaporated in vacuo. The residue was further purified by chromatography on SPE (silica, 0.5g) eluting with cyclohexane:ethyl acetate (2:1 followed by 1:1) to give Example 58 as a white solid (0.012g). LCMS showed MH+ = 399; TRET = 2.78min

    [0641] Similarly prepared using the same or similar numbers of moles of reagents and/or volumes of solvents was the following:
     NR10R11Starting amineMH+TRET (min)
    Example 59

    Morpholine 426 2.56

    Reference Example 60: 1-Ethyl-N-methyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0642] 



    [0643] Reference Intermediate 53 (0.076g, 0.21mmol) in phosphorous oxychloride (3ml) was heated at 120 °C for 3 hours then evaporated in vacuo. The residue was partitioned between DCM and water and the organic phase concentrated in vacuo. The residue was purified by mass directed autoprep HPLC to afford Reference Example 60 (0.027g). LCMS showed MH+ = 343; TRET = 2.34min.

    Example 61: trans-4-{[1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-yl]amino}cyclohexanol



    [0644] 



    [0645] Intermediate 54 (0.072g, 0.63mmol), Intermediate 12 (0.150g, 0.57mmol) and diisopropylethylamine (0.51ml) in acetonitrile (3ml) were heated at 85 °C for 18 hours then evaporated in vacuo. The residue was partitioned between DCM and water and the organic phase concentrated in vacuo. The residue was purified by mass directed autoprep HPLC to afford Example 61 (0.004g). LCMS showed MH+ = 343; TRET = 2.48min

    [0646] Similarly prepared using the same or similar numbers of moles of reagents and/or volumes of solvents were the following:
     NR3R3aAmine R3R3aNH
    (instead of Intermediate 54)
    MH+TRET (min)
    Example 62

    Intermediate 55 329 2.59
    Example 63

    Intermediate 56 341 2.53
    Reference Example 64

    Reference Intermediate 57 371 2.60

    Example 65: 5-[5-(1,1-Dimethylethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-6-methyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0647] 



    [0648] Intermediate 61 (0.266g, 0.56mmol) in phosphorous oxychloride (10ml) was heated at 120 °C for 1.5 hours then evaporated in vacuo. The residue was partitioned between DCM and water and the organic phase concentrated in vacuo. The residue was purified on an SPE cartridge (silica, 5g) eluting with cyclohexane: ethyl acetate (2:1, 1:1 then 2:3) to afford Example 65 (0.042g). LCMS showed MH+ = 385; TRET = 3.05min.

    Example 66: 1-Ethyl-6-methyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0649] 



    [0650] Intermediate 64 (0.05g, 0.11mol) and Burgess Reagent (0.053g, 0.22mol) in a mixture of THF / DMF (1ml, 1:1) were heated under microwave conditions at 120 °C (120W) for 5 minutes. The reaction mixtures were heated at 150 °C for four 10 minutes intervals with an additional portion of Burgess reagent (0.025g) being added after the first and third period of additional microwave heating. The reaction mixture was concentrated in vacuo and purified by SPE (silica, 0.5g) eluting with cyclohexane, cyclohexane: ethyl acetate (2:3 then 1:4) then ethyl acetate. Fractions containing the desired material were evaporated in vacuo to afford Example 66 (0.010g). LCMS showed MH+ = 413; TRET = 2.63min.

    Example 67: 5-(5-Cyclobutyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin4-amine



    [0651] 



    [0652] Intermediate 65 (0.05g, 0.13mmol) and Burgess Reagent (0.07g, 0.3mmol) in THF (2ml) was heated at 80 °C for 7 hours. The reaction mixture was concentrated in vacuo and a further portion of Burgess Reagent (0.07g, 0.3mmol) in THF (0.5ml) was added and the reaction mixture refluxed for 18 hours. The reaction was concentrated in vacuo and partitioned between DCM and water. The phases were separated using a hydrophobic frit (Whatman PTFE Filter Media with Polypropylene Housing 5µM pore size). The organic phase was concentrated in vacuo and the residue purified by mass directed autoprep HPLC to afford Example 67 (0.018g). LCMS showed MH+ = 369; TRET = 3.03min.

    [0653] Similarly prepared using the same or similar numbers of moles of reagents and/or volumes of solvents were the following:
     RYStarting IntermediateMH+ 398TRET (min)
    Example 68

    Intermediate 66 398 2.34
    Example 69

    Intermediate 67 386 2.29
    Example 70

    Intermediate 68 412 2.03
    Example 71

    Intermediate 69 411 2.92
    Example 72

    Intermediate 70 397 2.67
    Example 73

    Intermediate 71 385 2.65
    Example 74

    Intermediate 72 416 2.59
    Example 75

    Intermediate 73 383 3.22
    Example 76

    Intermediate 74 400 2.38
    Example 77

    Intermediate 75 413 2.79
    Example 78

    Intermediate 76 383 3.22
    Example 79

    Intermediate 77 396 2.88
    Example 80

    Intermediate 78 395 2.91

    Example 77: 1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0654] 


    Alternative Procedure:



    [0655] Burgess Reagent (0.168g, 0.74mmol) was added to a solution of Intermediate 75 (0.141 g, 0.33mmol) in tetrahydrofuran (2ml). The reaction mixture was heated at reflux for 1.5 hours then evaporated. The residue was applied to an SPE cartridge (silica, 10g) and eluted with cyclohexane:ethyl acteate (gradient of 0 to 100% ethyl acetate over 15 minutes at 15ml/min) followed by ethyl acetate then ethyl acetate:methanol (4:1). Appropriate fractions were combined and evaporated to give Example 77 as a white solid (0.099g). LCMS showed MH+ = 413, TRET = 2.72min. 1H NMR (400MHz in CDCl3, 27°C, δppm) 9.12 (br m, 1H), 8.72 (s, 1H), 8.01 (s, 1H), 4.52 (q, 2H), 4.245 (m, 1H), 4.08 (m, 2H), 4.00 (m, 2H), 3.67 (m, 2H), 3.44 (m, 2H), 2.91 (m, 2H), 2.20 (m, 3H), 1.93-1.70 (m, 4H), 1.57-1.40 (m, 5H).

    Example 81: 5-[5-(1-Acetyl-4-piperidinyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0656] 



    [0657] Intermediate 79 (0.18mmol) and Burgess Reagent (0.14g, 0.6mmol) in THF (0.75ml) was heated at 80 °C under an atmosphere of nitrogen for 16 hours. The reaction was concentrated using a stream of nitrogen and the residue dissolved in DCM (8ml). The solution was stirred with water and the phases separated using a hydrophobic frit (Whatman). The organic phase was concentrated in vacuo and the material was purified by mass directed auotprep HPLC to afford Example 81 (0.005g). LCMS showed MH+ = 440; TRET = 2.52min.

    Example 82: 1-Ethyl-5-{3-[(4-methyl-1-piperazinyl)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0658] 



    [0659] A mixture of Intermediate 16 (0.064g, 0.2mmol), Intermediate 80 (0.172g, 1mmol), a solution of sodium ethoxide in EtOH (0.19ml, 21% solution) and powdered 4Å molecular sieves (0.3 8g) in EtOH (2ml) were stirred at 82 °C under an atmosphere of nitrogen for 18 hours. The reaction mixture was filtered, the solvent was evaporated in vacuo and the residue was applied to an SPE cartridge (silica, 2g). The cartridge was eluted with (i) cyclohexane, (ii) cyclohexane: ethyl acetate (4:1, 3:2, 1:1, 2:3, 1:4), (iii) EtOAc, (iv) MeOH and (v) 10% aqueous NH3 solution in MeOH to afford Example 82 as a white solid (0.038g). LCMS showed MH+ =427; TRET = 2.10mm.

    [0660] Similarly prepared from Intermediate 16, using the same or similar numbers of moles of reagents and/or volumes of solvents, were the following:
     RXStarting Intermediate
    (instead of Intermediate 80)
    MH+TRET (min)
    Example 83

    Intermediate 81 398 2.34
    Example 84

    Intermediate 82 426 2.6

    Examples 85 to 96 - various 5-{3-[substituted]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amines



    [0661] Examples 85 to 96 can be prepared from Intermediate 16 using a similar processes to those described for any of Examples 28-31 or 82-84, using a similar or the same number of moles of reagents and/or volumes of solvents.

    [0662] Alternatively, Examples 85 to 90 and Examples 95 to 96 (all amides) can be prepared from the corresponding carboxylic acid compound Intermediate 83, by activating the carboxylic acid moiety (e.g. using a coupling agent such as EDC, HATU or more preferably TBTU) and reacting the activated carboxylic acid with the appropriate amine R10R11NH. This reaction, preferred reagents, and the structure of Intermediate 83 is shown in the following scheme (Intermediate 83 has the same structure as Example 84 but the 1,2,4-oxadiazole side-chain RX is -CH2-C(O)OH):



    [0663] As shown in the scheme above, Intermediate 83 can be prepared by hydrolysis of the corresponding t-butyl ester compound Intermediate 84 (wherein the 1,2,4-oxadiazole side-chain RX is -CH2-C(O)-O-tBu). Intermediate 84 can be prepared from Intermediate 17 and Intermediate 85 as shown in the scheme above. The preparation of Intermediate 85 has been shown earlier.

    [0664] In an alternative embodiment, Examples 85 to 90 and Examples 95 to 96 can be prepared from reaction of carboxylic acid Intermediate 83 with R10R11NH as shown above, but the Intermediate 83 (wherein the 1,2,4-oxadiazole side-chain RX is -CH2-C(O)OH) might be preparable from Example 84, by hydrolysing the amide bond within RX in Example 84 to form the carboxylic acid Intermediate 83.

    [0665] The example numbers and corresponding structures of Examples 85 to 96 are as follows:

    Example 85: 2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-phenylacetamide



    [0666] 


    General Procedure for Examples 85 to 90:



    [0667] N,N-Dimethylformamide (0.1ml) was added dropwise to a stirred mixture of Intermediate 83 (0.525g, 1.40mmol) and oxalyl chloride (0.18ml, 2.1mmol) in dichloromethane (15ml) at 0 °C under an atmosphere of nitrogen. The resultant mixture was stirred at 0 °C for 1 hour.

    [0668] An aliquot of the above solution (1.1ml) was added to a solution of the amine R10R11NH (0.6mmol) in dichloromethane (0.5ml). The reaction mixture was allowed to stand at room temperature for 2 hours then applied to an SPE cartridge (aminopropyl, 2g). The cartridge was eluted with chloroform then ethyl acetate / methanol (9:1). Fractions containing the product were concentrated and the residue purified by SPE cartridge (silica, 5g) eluting with dichloromethane, ether, ethyl acetate then ethyl acetate / methanol (9: 1). The desired fractions were concentrated to afford the examples given below.

    Example NumberNR10R11Source of Starting Amine R10R11NHMH+TRET (min)
    85

    Sigma-Aldrich 448 2.98
    86

    Sigma-Aldrich 476 2.97
    87

    Sigma-Aldrich 440 2.81
    88

    Sigma-Aldrich 461 2.90
    89

    Sigma-Aldrich 400 2.51
    90

    Sigma-Aldrich 400 2.51

    Example 92: 1-Ethyl-5-{3-[1-(4-morpholinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran4-yl)-1H-pyrazolo[3,4-b]pyridin4-amine



    [0669] 



    [0670] A mixture of Intermediate 16 (0.059g, 0.2mmol), Intermediate 121 (0.161g, 1.54mmol), a solution of sodium ethoxide in EtOH (0.21ml), 21% solution) and powdered 4Å molecular sieves (0.43g) in EtOH (1.5ml) were stirred at 82 °C under an atmosphere of nitrogen for 18 hours. The reaction mixture was filtered and the residue purified by mass directed autoprep HPLC to afford Example 92 (0.007g). LCMS showed MH+ = 428; TRET = 2.46min.

    Example 93: 5-[3-(Cyclohexylmethyl)-1,2,4-oxadiazol-5-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0671] 



    [0672] A mixture of Intermediate 16 (0.098g, 0.31mmol), Intermediate 122 (0.24g, 0.93mmol), a solution of sodium ethoxide in EtOH (0.21ml, 21% solution) and powdered 4Å molecular sieves (0.43g) in EtOH (1.5ml) were stirred at 82 °C under an atmosphere of nitrogen for 18 hours. The reaction mixture was filtered and the residue purified by mass directed autoprep HPLC to afford Example 93 (0.079g). LCMS showed MH+ = 411; TRET = 3.80min.

    Example 95: 1-Ethyl-5-{3-[2-oxo-2-(1-piperidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0673] 


    General Procedure for Example 95 to 96:



    [0674] N,N-Dimethylformamide (0.1ml) was added dropwise to a stirred mixture of Intermediate 83 (0.525g, 1.40mmol) and oxalyl chloride (0.18ml, 2.1mmol) in dichloromethane (15ml) at 0 °C under an atmosphere of nitrogen. The resultant mixture was stirred at 0 °C for 1 hour.

    [0675] An aliquot of the above solution (1.1ml) was added to a solution of the R10R11NH amine (0.6mmol) in dichloromethane (0.5ml). The reaction mixture was allowed to stand at room temperature for 2 hours then applied to an SPE cartridge (aminopropyl, 2g). The cartridge was eluted with chloroform then ethyl acetate / methanol (9:1). Fractions containing the product were concentrated and the residue purified by SPE cartridge (silica, 5g) eluting with dichloromethane, ether, ethyl acetate then ethyl acetate / methanol (9:1). The desired fractions were concentrated to afford the examples given below.

    Example NumberNR10R11Source of Starting Amine R10R11NHMH+TRET (min)
    95

    Sigma-Aldrich 442 2.51
    96

    Sigma-Aldrich 455 2.08

    Examples 97 to 125 - various 5-{5-[substituted]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amines



    [0676] Examples 97 to 125 can be made using processes similar to those described for any of Examples 9, 14, 32-40, 44-45, 60-64, 65-66, and 67-81, using a similar or the same number of moles of reagents and/or volumes of solvents.

    Example 97: 1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(1H-1,2,3-triazol-1-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0677] 


    General Procedure for preparation of Examples 97 to 125:



    [0678] A mixture of diacyl hydrazide Intermediate (one of Intermediates 89-114) and Burgess Reagent (2 equivalents) in N,N-dimethylformamide (1ml) was heated in a microwave for 10 minutes at 120 °C at 150 Watts. The resultant solution was concentrated in vacuo and partitioned between chloroform and water. The organic phase was separated using a hydrophobic frit (Whatman PTFE Filter Media with Polypropylene Housing 5µM pore size) then concentrated. The residue was purified by mass directed auto-prep BPLC.

    [0679] As either formic acid or trifluoroacetic acid are used in the solvents in the mass directed auto-prep HPLC procedure (see "Machine Methods section hereinbefore), some of the Examples were isolated as the formate salt or trifluoroacetate salt as shown below.

    [0680] The example numbers and corresponding structures of Examples 97 to 125 are as follows:

    Example NumberRYDiacyl hydrazide Intermediate numberMH+TRET (min)
    97

    89 396 2.47
    98 (as formate salt)

    90 440 2.79
    99 (as formate salt)

    91 395 2.97
    100 (as formate salt)

    92 396 2.69
    103 (as trifluoro-acetate salt)

    93 435 3.15
    104 (as formate salt)

    94 397 2.51
    105

    95 412 2.79
    106 (as formate salt)

    96 410 2.77
    107

    97 448 3.01
    108 (as formate salt)

    98 426 2.76
    109 (as trifluoroacetate salt)

    99 468 2.85
    111

    100 412 2.53
    112 (as formate salt)

    101 396 3.03
    113

    102 410 2.81
    114 (as formate salt)

    103 440 2.79
    117 (as trifluoro-acetate salt)

    106 424 2.80
    118

    107 400 2.43
    119 (as trifluoro-acetate salt)

    108 454 2.63
    120

    109 419 3.20
    121

    110 405 3.41
    122

    111 419 3.53
    123

    112 419 3.65
    125

    114 3.30 483/485

    Examples 126 to 147 - various 5-{4-[substituted]-oxazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amines



    [0681] Examples 126 to 147 (all amides) can be prepared by reacting Example 57 and the appropriate amine to form the amide bond using a process similar to that described for Example 58, except that HATU is preferably used instead of EDC as coupling agent, and using a similar or the same number of moles of reagents and/or volumes of solvents as in Example 58.

    Example 126: 2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(phenylmethyl)-1,3-oxazole-4-carboxamide



    [0682] 


    General Procedure for Examples 126 to 147:



    [0683] A mixture of Example 57 (0.014g, 0.04mmol), diisopropylethylamine (0.0017ml, 0.096mmol) and HATU (0.016g, 0.042mmol) in N,N-dimethylformamide (0.4ml) was allowed to stand for 10 minutes. The resultant solution was added to the appropriate amine R10R11NH (0.05mmol) and mixture agitated by sonication. After standing for 18 hours the solvent was removed in vacuo. The residue was applied to an SPE cartridge (aminopropyl, 0.5g) and the cartridge eluted with chloroform (1.5ml) followed by ethyl acetate : methanol (9:1, 2ml). Appropriate fractions were evaporated in vacuo and the residue purified by mass directed auto-prep HPLC

    [0684] The example numbers and corresponding structures of Examples 126 to 147 are as follows:

    Example NumberNR10R11Source of Starting Amine R10R11NHMH+TRET (min)
    126

    Sigma-Aldrich 447 3.14
    127

    Sigma-Aldrich 477 3.16
    128

    Sigma-Aldrich 461 3.26
    129

    Sigma-Aldrich 461 3.36
    130

    Sigma-Aldrich 461 3.23
    131

    Sigma-Aldrich 481 3.35
    132

    Matrix Scientific or Maybridge 475 3.57
    133

    Matrix Scientific 475 3.55
    134

    Matrix Scientific or Pfaulz-Bauer 475 3.46
    135

    Sigma-Aldrich 461 3.21
    136

    Pfaulz-Bauer 491 3.22
    137

    Sigma-Aldrich 475 3.32
    138

    Sigma-Aldrich 447 3.42
    139

    J.Med.Chem. 2003, 46(14), 3116 540 2.87
    140

    WO 02/016318 525 2.86
    141

    Intermediate 25 482 2.55
    142

    Intermediate 21 441 2.68
    143

    Sigma-Aldrich 441 2.89
    144

    J.Med.Chem. 1999, 42(15), 2870 or Matrix Scientific 483 2.21
    145

    WO 96/05166 482 3.32
    146

    Sigma-Aldrich 461 3.23
    147

    Intermediate 115 490 2.58

    Example 148: 2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(tetrahydro-2H-pyran-4-yl)acetamide



    [0685] 


    General Procedure for Examples 148-155 and for alternative preparation of Example 84:



    [0686] N,N-Dimethylformamide (0.1ml) was added dropwise to a stirred mixture of Intermediate 83 and oxalyl chloride (0.18ml, 2.1mmol) in dichloromethane (15ml) at 0 °C under an atmosphere of nitrogen. The resultant mixture was stirred at 0 °C for 1 hour.

    [0687] An aliquot of the above solution (1.1ml) was added to a solution of the amine R10R11NH (0.6mmol) in dichloromethane (0.5ml). The reaction mixture was allowed to stand at room temperature for 2 hours then applied to an SPE cartridge (aminopropyl, 2g). The cartridge was eluted with chloroform then ethyl acetate / methanol (9:1). Fractions containing the product were concentrated and the residue purified by SPE cartridge (silica, 5g) eluting with dichloromethane, ether, ethyl acetate then ethyl acetate / methanol (9:1). The desired fractions were concentrated to afford the examples given below.

    Example NumberNR10R11Source of Amine R10R11NHMH+TRET (min)
    148

    Intermediate 21 456 2.49
    149

    Sigma-Aldrich (mixture of isomers) 470 2.66,2.71
    150

    Sigma-Aldrich 454 2.96
    152

    Sigma-Aldrich 444 2.57
    153

    Sigma-Aldrich 468 3.13
    154

    Sigma-Aldrich 454 2.96
    155

    Sigma-Aldrich 449 2.51
    84 (alternative preparation)

    Sigma-Aldrich 426 2.63

    Example 157: 6-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylannino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-2-piperidinone



    [0688] 


    General Procedure for Examples 157 to 158:



    [0689] A mixture of diacyl hydrazide Intermediate 104 or 105 and Burgess Reagent (2 equivalents) in N,N-dimethylformamide (1ml) was heated in a microwave for 10 minutes at 120 °C at 150 Watts. The resultant solution was concentrated in vacuo and partitioned between chloroform and water. The organic phase was separated using a hydrophobic frit (Whatman PTFE Filter Media with Polypropylene Housing 5µM pore size) then concentrated. The residue was purified by mass directed auto-prep HPLC
    Example NumberRYDiacyl hydrazide IntermediateMH+TRET (min)
    157

    Intermediate 104 411 2.45
    158

    Intermediate 105 409 2.38

    Example 159: N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)acetamide



    [0690] 


    General Procedure for Examples 159 to 165:



    [0691] The appropriate carboxylic acid chloride R17C(O)Cl (0.12mmol) was added to a stirred solution of amine Intermediate 118 (0.1mmol) and diisopropylethylamine (0.3mmol) in chloroform (1ml) at room temperature. After stirring at room temperature for 16h, the reaction mixture was applied to a SPE cartridge (aminopropyl, 2g) and the cartridge was eluted sequentially with chlororm, ethyl acetate and methanol. Fractions containing the desired product were combined and blown down under nitrogen. The resulting residue was further purified on a SPE cartidge (silica, 1g) eluting with a gradient of 30-100% ethyl acetate in petroleum ether. Appropriate fractions were combined and the solvents were evaporated to afford the product.

    Example NumberR17Source of Acyl chloride R17C(O)ClMH+TRET (min)
    159 CH3 Sigma-Aldrich 386 2.38
    160

    Sigma-Aldrich 448 2.82
    161

    Sigma-Aldrich 462 2.85
    162

    Sigma Aldrich 414 2.66
    163

    Sigma-Aldrich 428 2.79
    164

    Sigma-Aldrich 454 2.99
    165

    Lancaster 438 2.68

    Example 166: N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)methanesulfonamide



    [0692] 


    General Procedure for Examples 166 to 172:



    [0693] The appropriate sulphonyl chloride R18S(O)2Cl (0.12mmol) was added to a stirred solution of amine Intermediate 118 (0.1mmol) and pyridine (0.2mmol) in chloroform (1ml) at room temperature. After stirring at room temperature for 16h, the reaction mixture was applied to a SPE cartridge (aminopropyl, 2g,) and the cartridge was eluted sequentially with chlororm, ethyl acetate and methanol. Fractions containing the desired product were combined and blown down under nitrogen. The resulting residue was further purified on a SPE cartidge (silica, 1g) eluting with a gradient of 30-100% ethyl acetate in petroleum ether. Appropriate fractions were combined and the solvents were evaporated to afford the product.

    Example NumberR18Source of Sulphonyl chloride R18S(O)2ClMH+TRET (min)
    166 CH3 Sigma-Aldrich 422 2.59
    167

    Sigma-Aldrich 484 3.00
    168

    Sigma-Aldrich 498 3.04
    169

    Sigma-Aldrich 450 2.79
    170

    Sigma-Aldrich 450 2.83
    171

    Array Biopharma Inc 448 2.69
    172

    Avocado 490 2.93

    Example 173: 1-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-pyrrolidinone



    [0694] 



    [0695] A solution of Intermediate 119 (45mg, 0.1mmol) in dry dimethylformamide (2ml) was added to sodium hydride (60% dispersion in mineral oil, 4.4mg, 0.11mmol), and the resulting mixture was stirred at room temperature. After 16h, the reaction mixture was diluted with water (2ml) and extracted with chloroform (3 x 5ml). The combined organic extracts were dried over anhydrous sodium sulphate and evaporated to give the crude product. Purification of the crude product on a SPE cartridge (silica, 2g) using a gradient of ethyl acetate in petroleum ether afforded Example 173. LCMS showed MH+ =412, TRET = 2.59min.

    Example 1740: 1-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-piperidinone



    [0696] 



    [0697] A solution of Intermediate 120 (46mg, 0.1mmol) in dry dimethylformamide (2ml) was added to sodium hydride (60% dispersion in mineral oil, 4.4mg, 0.11mmol), and the resulting mixture was stirred at room temperature. After 16h, the reaction mixture was diluted with water (2ml) and extracted with chloroform (3 x 5ml). The combined organic extracts were dried over anhydrous sodium sulphate and evaporated to give the crude product. Purification of the crude product on a SPE cartridge (silica, 2g) using a gradient of ethyl acetate in petroleum ether afforded Example 174. LCMS showed MH+ =426, TRET = 2.66min.

    Example 175 5-{3-[(1-Acetyl-4-piperidinyl)methyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0698] 



    [0699] Acetyl chloride (0.04mmol) was added to a stirred solution of Intermediate 125 (0.033mmol) and diisopropylethylamine (0.1mmol) in chloroform (1ml) at room temperature. After stirring at room temperature for 1.5h, a further quantity of acetyl chloride (0.04mmol) and diisopropylethylamine (0.1mmol) were added to the reaction mixture. After 3.5h the reaction mixture was applied to a SPE cartridge (aminopropyl, 1g,) and the cartridge was eluted sequentially with chlororm, ethyl acetate and methanol. Fractions containing the desired product were combined and blown down under nitrogen. The resulting residue was further purified on a SPE cartridge (silica, 1g) eluting with a gradient of 30-100% ethyl acetate in petroleum ether to afford Example 175 LCMS showed MH+ = 454, TRET = 2.79min.

    Example 176 1-Ethyl-5-(3-{[1-(3-methylbutanoyl)-4-piperidinyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0700] 



    [0701] Isovaleryl chloride (0.04mmol) was added to a stirred solution of Intermediate 125 (0.033mmol) and diisopropylethylamine (0.1mmol) in chloroform (1ml) at room temperature. After stirring at room temperature for 1.5h, the reaction mixture was applied to a SPE cartridge (aminopropyl, 1g,) and the cartridge was eluted sequentially with chloroform, ethyl acetate and methanol. Fractions containing the desired product were combined and blown down under nitrogen. The resulting residue was further purified on a SPE cartridge (silica, 1g) eluting with a gradient of 30-100% ethyl acetate in petroleum ether to afford Example 176. LCMS showed MH+ = 496, TRET = 3.17min.

    Example 177: 1-Ethyl-5-(3-{[1-(methylsulfonyl)4-piperidinyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0702] 



    [0703] Methanesulphonyl chloride (1.16mmol) was added to a stirred solution of Intermediate 125 (0.033mmol) and pyridine (0.5ml) in chloroform (1ml) at room temperature. After stirring at room temperature for 31h, the reaction mixture was applied to a SPE cartridge (aminopropyl, 5g) and the cartridge was eluted sequentially with chloroform, ethyl acetate and methanol. Fractions containing the desired product were evaporated in vacuo. The resulting residue was further purified on a SPE cartridge (silica, 1g) eluting with a gradient of 30-100% ethyl acetate in petroleum ether to afford Example 176. LCMS showed MH+ = 490, TRET = 2.97min.

    Example 178:



    [0704] 



    [0705] A mixture of Intermediate 16 (0.067g, 0.26mmol), amidoxime Intermediate 126 (0.255g, 1.06mmol), a solution of sodium ethoxide in EtOH (0.87ml), 21 % solution) and powdered 4Å molecular sieves (0.68g) in EtOH (2ml) were stirred at 82 °C under an atmosphere of nitrogen for 12 hours. The reaction mixture was filtered and the solvent was evaporated in vacuo. The residue was applied to an SPE cartridge (silica, 5g) and eluted with ethyl acetate: cyclohexane (0 to 70% in 10% increments). Appropriate fractions were combined and evaporated, the residue was purified further by mass directed auto prep HPLC to give Example 178 (0.011g) LCMS showed MH+ = 495; TRET = 3.2min.

    [0706] Similarly prepared using the same or similar numbers of moles of reagents and/or volumes of solvents were the following:

    Example NumberRXAmidoxime Intermediate number (instead of Intermediate 126)MH+TRET (min)
    179

    127 405 3.35
    180

    128 419 3.44
    181

    129 435 3.34
    182

    130 435 3.35
    183

    132 448 3.2
    184

    131 448 3.30
    185

    133 421 3.35
    186

    134 450 2.47
    187

    135 489 3.01

    Example 188: 1-Ethyl-5-(5-ethyl-1,2,4-oxadiazol-3-yl)-N-(tetrahydro-2H-pyran4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0707] 



    [0708] Propionic anhydride (0.01 5ml), 0.12mmol) was added to Intermediate 138 (0.030g, 0.1mmol) in glacial acetic acid (1.5ml). The reaction mixture was stirred at room temperature for 2 hours then heated at 80 °C for 5 hours. The solvent was concentrated in vacuo and the residue applied to an SPE cartridge (silica, 1g). The cartridge was eluted with cyclohexane then cyclohexane:ethyl acetate (7:3). Appropriate fractions were combined and evaporated to give Example 188 as a white solid (0.015g). LCMS showed MH+ = 343; TRET = 2.92min

    Example 189: 5-(5-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-3-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0709] 



    [0710] 4-(Dimethylamino)phenylacetic acid (0.09g, 0.504mmol) and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (0.097g, 0.51mmol) in dichloromethane (1ml) were stirred at room temperature for 3 hours. The reaction mixture was concentrated then Intermediate 138 (0.07g, 0.23mmol) and diglyme (1ml) were added. After stirring at 20 C for 18 hour glacial acetic acid (0.07ml) and additional diglyme (0.5ml) were added and the mixture heated at 60 °C for 2 hours then at 75 °C for 4 hours. The reaction mixture was applied to an SPE cartridge (SCX, 2g) and the cartridge eluted with methanol then 10% ammonia in methanol. The methanolic ammonia fractions were evaporated in vacuo and the residue purified by mass directed autoprep HPLC to afford Example 189 as a beige solid (0.004g). LCMS showed MH+ = 448; TRET = 3.24min.

    Example 190: 1-Ethyl-5-(5-{[4-(methyloxy)phenyl]methyl}-1,2,4-oxadiazol-3-yl)-N-(tetrahydro-2H-pyran4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0711] 



    [0712] Prepared from Intermediate 138 and 4-methoxyphenylacetic acid using a similar process to that described for Example 189 using similar or the same number of moles of reagents and/or volumes of solvents. LCMS showed MH+ = 435; TRET = 3.26min

    Example 191: 5-(3,8-Dioxa-1-azaspiro[4.5]dec-1-en-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine



    [0713] 



    [0714] Example 191 was prepared from Intermediate 139 using an analogous method to that for Example 49. LCMS showed MH+ = 386, TRET = 2.71min.


    Claims

    1. A compound of formula (I) or a salt thereof:

    wherein:

    R1 is C1-3alkyl, C1-2fluoroalkyl or -(CH2)2OH;

    R2 is a hydrogen atom (H), methyl or C1fluoroalkyl;

    R3 is optionally substituted C3-8cycloalkyl, or an optionally substituted heterocyclic group of sub-formula (aa), (bb) or (cc):

    in which n1 and n2 independently are 1 or 2; and Y is O, S, SO2, or NR4; where R4 is a hydrogen atom (H), C1-2alkyl, C1-2fluoroalkyl, C(O)NH2, C(O)-C1-2alkyl, or C(O)-C1fluoroalkyl;
    wherein in R3 the C3-8cycloalkyl is optionally substituted with one or two substituents independently being oxo (=O); OH; C1alkoxy; C1fluoroalkoxy; NHR21 wherein R21 is a hydrogen atom (H); C1-2alkyl; C1fluoroalkyl; -CH2OH; -CH2NHR22 wherein R22 is H; -C(O)OR23 wherein R23 is H; -C(O)NHR24 wherein R24 is H; -C(O)R25 wherein R25 is methyl; fluoro; hydroxyimino (=N-OH); or (C1-2alkoxy)imino (=N-OR26 where R26 is C1-2alkyl); and wherein any OH, alkoxy, fluoroalkoxy or NHR21 substituent is not substituted at the R3 ring carbon attached (bonded) to the -NH- group of formula (I);
    and wherein in R3 the heterocyclic group of sub-formula (aa), (bb) or (cc) is optionally substituted with one or two substituents being oxo (=O);
    and R3a is a hydrogen atom (H);
    and wherein Het is of sub-formula (i), (ii), (iii), (iv) or (v):

    wherein:

    W1, W2, W4 and W5 are N; and W3 is NRW;

    X1, X3 and X4 are N or CRX; X2 is O, S or NRX; and X5 is CRX1RX2 or CRX3RX4;

    Y1 Y2 and Y3 are CRY or N; Y4 is O, S or NRY; and Y5 is CRY1RY2;

    Z1 and Z5 are O, S or NRZ; and Z2, Z3 and Z4 are N or CRZ;

    wherein:

    RW is a hydrogen atom (H);

    RX, RX2, RY and RY2 independently are:

    a hydrogen atom (H);

    C1-8alkyl;

    C3-6cycloalkyl optionally substituted by one or two C1-2alkyl groups and/or by one oxo (=O) group;

    -(CH2)n2a-C3-6cycloalkyl optionally substituted, in the -(CH2)n2a- moiety or in the C3-6cycloalkyl moiety, by a C1-2alkyl group, or optionally substituted in the C3-6cycloalkyl moiety by a -CH2C(O)NHC1-2alkyl group, wherein n2a is 1, 2 for 3;

    -(CH2)n3-S(O)2-R5, -CH(C1-2alkyl)-S(O)2-R5, -CMe2-S(O)2-R5, or C3-5cycloalkyl substituted at the connecting carbon atom by -S(O)2-R5,
    wherein n3 is 1 or 2;
    and R5 is C1-4alkyl, -NR15R16, phenyl, carbon-linked-pyridinyl or benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two substituents independently being fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy, C1fluoroalkoxy or OH, and wherein the pyridinyl is optionally substituted by one methyl, methoxy or OH (including any tautomer thereof));
    wherein R15 is H, C1-4alkyl, phenyl, benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two substituents independently being fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy), CH(Me)Ph, or carbon-linked-pyridinyl optionally substituted by one methyl, methoxy or OH (including any tautomer thereof);
    and R16 is H or C1-2alkyl;
    or wherein R15 and R16 together are -(CH2)n3a-X3a-(CH2)n3b- in which n3a and n3b independently are 2 or 3 and X3a is a bond, -CH2-, O, or NR8a wherein R8a is H or C1-2alkyl, acetyl, -S(O)2Me or phenyl, and wherein the ring formed by NR15R16 is optionally substituted on a ring carbon by one or two substituents independently being methyl or oxo (=O);

    -(CH2)n4-NR6R7, -CH(C1-2alkyl)-NR6R7, -CMe2-NR6R7, or C3-5cycloalkyl substituted at the connecting carbon atom by -NR6R7, wherein n4 is 0, 1, 2 or 3;
    and R6 and R7 independently are H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, -C(O)R17, -S(O)2R18, phenyl, benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two substituents independently being fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy), or carbon-linked-pyridinyl optionally substituted by one methyl, methoxy or OH (including any tautomer thereof);
    and wherein R17 and R18 independently are C1-6alkyl, C3-6cycloalkyl, optionally substituted 5-membered heteroaryl being furyl (furanyl) or 1,3-oxazolyl or isoxazolyl or oxadiazolyl or thienyl or 1,3-thiazolyl or isothiazolyl or pyrrolyl or imidazolyl or pyrazolyl (all independently optionally substituted by one oxo and/or one or two methyl), or phenyl or benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two substituents independently being fluoro, chloro, C1-2alkyl, C1 fluoroalkyl, C1-2alkoxy, C1fluoroalkoxy or OH), or carbon-linked-pyridinyl optionally substituted by one methyl, methoxy or OH (including any tautomer thereof);
    or R6 and R7 together are -(CH2)n5-X5-(CH2)n6- in which n5 and n6 independently are 2 or 3 and X5 is a bond, -CH2-, O, or NR8 wherein R8 is H, C1-2alkyl, acetyl, -S(O)2Me or phenyl, and wherein the ring formed by NR6R7 is optionally substituted on a ring carbon by one or two substituents independently being methyl or oxo (=O);

    -(CH2)n7-O-R9; wherein n7 is 0, 1, 2 or 3 and R9 is H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, -C(O)R17, phenyl, or benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy); wherein n7 is 0 only when the -(CH2)n7-O-R9 is bonded to a carbon atom in the Het ring; and wherein n7 is not 0 when Het is of sub-formula (v) (i.e. n7 is not 0 for RX2 and for RY2);

    -(CH2)n11-C(O)-NR10R11, -CH(C1-2alkyl)-C(O)-NR10R11,
    -CMe2-C(O)-NR1OR11, or C3-5cycloalkyl substituted at the connecting carbon atom by -C(O)-NR10R11, wherein n11 is 0, 1 or 2;
    and wherein R10 and R11 independently are H; C1-6alkyl; C1-4fluoroalkyl; C2-4alkyl substituted by one OH or -OC1-2alkyl other than at the connection point; C3-6cycloalkyl optionally substituted by one or two methyl groups; -CH2-C3-6cycloalkyl optionally substituted by one methyl, NH2 or NHMe group; -(CH2)n17-Het2; carbon-linked-pyridinyl optionally substituted by one methyl, methoxy or OH (including any tautomer thereof); phenyl; benzyl; or -CH(C1-2alkyl)Ph [wherein the phenyl, benzyl and
    -CH(C1-2alkyl)Ph are independently optionally substituted on the aromatic ring by one or two substituents independently being: fluoro, chloro,
    C1-2alkyl, C1fluoroalkyl, C1-2alkoxy, C1fluoroalkoxy, OH, NR10aR10b (wherein R10a is H or C1-2alkyl and R10b is H, C1-2alkyl, -C(O)-C1-2alkyl or -S(O)2-C1-2alkyl), -C(O)-NR10cR10d (wherein R10c and R10d independently are H or C1-2alkyl), or -S(O)2-R10e (wherein R10e is
    C1-2alkyl, NH2, NHMe or NMe2)];

    wherein n17 is 0, 1 or 2 and wherein Het2 is a 4-, 5- or 6- membered saturated heterocyclic ring containing one O or S ring atom or one NR27 ring group wherein R27 is H, C1-2alkyl, -C(O)Me, or -S(O)2Me, wherein the Het2 ring is optionally substituted on a ring carbon by one or two substituents independently being methyl or oxo (=O);
    and wherein when n17 is 2 then the Het2 ring can optionally contain one additional ring N atom at the Het2 ring position bonded to the -(CH2)n17-moiety; provided that, when Het2 contains one O or S or NR27 ring atom/group and one additional ring N atom, then the O/S/NR27 ring atom/group and the one additional ring N atom are not directly bonded to each other, and are separated by more than one carbon atom;
    or R10 and R11 together are -(CH2)n8-X6-(CH2)n9- in which n8 and n9 independently are 2 or 3 and X6 is a bond, -CH2-, O, or NR12 wherein R12 is H, C1-2alkyl, acetyl, -S(O)2Me or phenyl, and wherein the ring formed by NR10R11 is optionally substituted on a ring carbon by one or two substituents independently being methyl or oxo (=O);

    - (CH2)n12-C(O)-OR13 wherein n12 is 0, I or 2; and wherein R13 is H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, phenyl, or benzyl (wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two of (independently) fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy);

    - (CH2)n13-C(O)-R13a wherein n13 is 0, 1 or 2; and wherein R13a is a hydrogen atom (H), C1-6alkyl, C1-2fluoroalkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, benzyl, or phenyl; wherein the phenyl and benzyl are independently optionally substituted on the aromatic ring by one or two of (independently) fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy;

    - (CH2)n14-Het1, -CH(C1-2alkyl)-Het1, -CMe2-Het1, or C3-5cycloalkyl substituted at the connecting carbon atom by Het1, wherein n14 is 0, 1 or 2 and wherein Het1 is a 4-, 5-, 6- or 7-membered saturated heterocyclic ring;

    wherein said heterocyclic ring Het1 contains one O or S ring atom and/or one NR14 ring group wherein R14 is H, C1-4alkyl, C3-6cycloalkyl, benzyl, phenyl, -C(O)R19, or -S(O)2R19;
    wherein R19, independent of any other R19, is C1-6alkyl, C3-6cycloalkyl, thienyl, furyl (furanyl), or phenyl or benzyl; wherein the phenyl and benzyl are independently optionally substituted by one or two of (independently) fluoro, methyl or methoxy;
    and wherein said heterocyclic ring Het1 is optionally substituted (at a position or positions other than any NR14 position) by one or two oxo (=O) and/or one C1-4alkyl substituents;
    provided that, when the heterocyclic ring Het1 contains one O or S ring atom and one NR14 ring group then: (a) the O/S ring atom and the NR14 ring group are not directly bonded to each other, and (b) the O/S ring atom and the NR14 ring group are separated by more than one carbon atom unless Het1 contains an -NR14-C(O)-O- or -NR14-C(O)-S- moiety as part of the ring; or

    - (CH2)n10-Ar, -CH(C1-2alkyl)-Ar, -CMe2-Ar, or C3-5cycloalkyl substituted at the connecting carbon atom by Ar, wherein n10 is 0, 1 or 2 and

    (i) Ar is phenyl optionally substituted by one or two substituents independently being fluoro, chloro, bromo, C1-2alkyl, C1-2fluoroalkyl, C1-2alkoxy, C1-2fluoroalkoxy, OH, -NR11aR11b (wherein R11a is H or C1-2alkyl and R11b is H, C1-2alkyl, -C(O)-C1-2alkyl or -S(O)2-C1-2alkyl), cyano, -C(O)-NR11cR11d (wherein R11c and R11d independently are H or C1-2alkyl), -C(O)-OR11e wherein R11e is H or C1-2alkyl, or -S(O)2-R11f (wherein R11f is C1-2alkyl, NH2, NHMe or NMe2); or the phenyl Ar is optionally substituted at two adjacent Ar ring atoms by the two ends of a chain which is: -(CH2)4-, -(CH2)3-, or -CH=CH-CH=CH-; or

    (ii) Ar is an optionally substituted 5- or 6-membered heterocyclic aromatic ring containing 1, 2, 3 or 4 heteroatoms selected from O, N or S; and wherein when the heterocyclic aromatic ring Ar contains 2, 3 or 4 heteroatoms, one is selected from O, N and S and the remaining heteroatom(s) are N; and wherein the heterocyclic aromatic ring Ar is optionally substituted by one or two groups independently being C1-4alkyl or OH (including any keto tautomer of an OH-substituted aromatic ring), or the heterocyclic aromatic ring Ar is optionally substituted at two adjacent Ar ring atoms by the two ends of a chain which is: -(CH2)4-, -(CH2)3-, or -CH=CH-CH-CH-;

    RX1 and RY1 independently are a hydrogen atom (H), C1-2alkyl or C1fluoroalkyl;

    RX3 and RX4 together are -(CH2)n15-X7-(CH2)n16- wherein n15 and n16 independently are 1 or 2 and X7 is a bond, -CH2-, O, or NRX5 wherein RX5 is H, C1-2alkyl, acetyl or -S(O)2Me; and

    RZ is a hydrogen atom (H);

    provided that:

    when R3 is the heterocyclic group of sub-formula (bb), n1 is 1, and Y is NR4, then R4 is not C1-2alkyl or C1-2fluoroalkyl;

    when R3 is the heterocyclic group of sub-formula (aa) and Y is NR4, then R4 is not C(O)NH2, C(O)-C1-2alkyl or C(O)-C1fluoroalkyl;

    provided that: when R3 is the heterocyclic group of sub-formula (cc), then NR3R3a is of sub-formula (L), (m), (m1) or (m2), wherein the -NH- connection point of the NR3R3a group to the 4-position of the pyrazolopyridine of formula (I) is underlined:

    and provided that:

    where R3 is optionally substituted C3-8cycloalkyl, then any OH, alkoxy, fluoroalkoxy, -CH2OH, -CH2NHR22, -C(O)OR23, -C(O)NHR24, -C(O)R25 or fluoro substituent is at the 3-, 4- or 5-position of the R3 cycloalkyl ring (wherein, in this connection, the 1-position of the R3 cycloalkyl ring is deemed to be the connection point to the -NH- in formula (I)).


     
    2. A compound or salt as claimed in claim 1 which is a compound of formula (IA) or a salt thereof:

    wherein:

    R1 is C1-3alkyl, C1-2fluoroalkyl or -(CH2)2OH;

    R2 is a hydrogen atom (H), methyl or C1 fluoroalkyl;

    R3 is optionally substituted C3-8cycloalkyl, or an optionally substituted heterocyclic group of sub-formula (aa), (bb) or (cc):

    in which n1 and n2 independently are 1 or 2; and Y is O, S, SO2, or NR4; where R4 is a hydrogen atom (H), C1-2alkyl, C1-2fluoroalkyl, C(O)NH2, C(O)-C1-2alkyl, or C(O)-C1 fluoroalkyl;
    wherein in R3 the C3-8cycloalkyl is optionally substituted with one or two substituents being oxo (=O), OH, C1alkoxy, C1fluoroalkoxy, or C1-2alkyl; and wherein any OH, alkoxy or fluoroalkoxy substituent is not substituted at the R3 ring carbon attached (bonded) to the -NH- group of formula (IA);
    and wherein in R3 the heterocyclic group of sub-formula (aa), (bb) or (cc) is optionally substituted with one or two substituents being oxo (=O);
    and wherein Het is of sub-formula (i), (ii), (iii), (iv) or (v):

    wherein:

    W1, W2, W4 and W5 are N; and W3 is NRW;

    X1, X3 and X4 are N or CRX; X2 is O, S or NRX ; and X5 is CRX1RX2;

    Y1, Y2 and Y3 are CRY or N; Y4 is O, S or NRY ; and Y5 is CRY1RY2;

    Z1 and Z5 are O, S or NRZ; and Z2, Z3 and Z4 are N or CRZ;

    wherein:

    RW is a hydrogen atom (H);

    RX, RX2, RY and RY2 independently are:

    a hydrogen atom (H);

    C1-8alkyl;

    C3-6cycloalkyl optionally substituted by a C1-2alkyl group;

    -(CH2)n2a-C3-6cycloalkyl optionally substituted, in the -(CH2)n2a- moiety or in the C3-6cycloalkyl moiety, by a C1-2alkyl group, wherein n2a is 1, 2 or 3;

    -(CH2)n3-SO2-R5 wherein n3 is 1 or 2 and R5 is C1-3alkyl or -NH-C1-2alkyl or phenyl;

    -(CH2)n4-NR6R7 wherein n4 is 0, 1, 2 or 3, and R6 and R7 independently are H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, -C(O)-C1-2alkyl, -SO2-C1-2alkyl, phenyl, or benzyl (wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy); or R6 and R7 together are -(CH2)n5-X5-(CH2)n6- in which n5 and n6 independently are 2 or 3 and X5 is a bond, -CH2-, O, or NR8 wherein R8 is H or C1-2alkyl;

    -(CH2)n7-O-R9; wherein n7 is 0, 1, 2 or 3 and R9 is H or C1-6alkyl; wherein n7 is 0 only when the -(CH2)n7-O-R9 is bonded to a carbon atom in the Het ring; and wherein n7 is not 0 when Het is of sub-formula (v) (i.e. n7 is not 0 for RX2 and for RY2);

    -C(O)-NR10R11 wherein R10 and R11 independently are H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, phenyl, or benzyl (wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy); or R10 and R11 together are -(CH2)n8-X6-(CH2)n9- in which n8 and n9 independently are 2 or 3 and X6 is a bond, -CH2-, O, or NR12 wherein R12 is H or C1-2alkyl;

    -C(O)-OR13 wherein R13 is H, C1-6alkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, phenyl, or benzyl (wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy);

    -C(O)-R13a wherein R13a is a hydrogen atom (H), C1-6akyl, C1-2fluoroalkyl, C3-6cycloalkyl, -CH2-C3-6cycloalkyl, benzyl, or phenyl; wherein the phenyl or benzyl are independently optionally substituted on the aromatic ring by one of fluoro, chloro, C1-2alkyl, C1fluoroalkyl, C1-2alkoxy or C1fluoroalkoxy;

    a 4-, 5-, 6- or 7-membered saturated heterocyclic ring containing one O ring atom or one NR14 ring group wherein R14 is H or C1-4alkyl, said heterocyclic ring being optionally substituted (at a position or positions other than any NR14 position) by one oxo (=O) and/or one C1-4alkyl substituent; or

    -(CH2)n10-Ar wherein n10 is 0, 1 or 2 and

    (i) Ar is phenyl optionally substituted by one or two substituents being fluoro, chloro, C1-2alkyl, C1-2fluoroalkyl, C1-2alkoxy, C1-2fluoroalkoxy or cyano;
    or

    (ii) Ar is an optionally substituted 5- or 6-membered heterocyclic aromatic ring containing 1, 2 or 3 heteroatoms selected from O, N or S; and wherein when the heterocyclic aromatic ring Ar contains 2 or 3 heteroatoms, one is selected from O, N and S and the remaining heteroatom(s) are N; and wherein the heterocyclic aromatic ring Ar is optionally substituted by one or two C1-4alkyl groups;

    RX1 and RY1 independently are a hydrogen atom (H), C1-2alkyl or C1 fluoroalkyl; and

    RZ is a hydrogen atom (H);

    provided that:

    when R3 is the heterocyclic group of sub-formula (bb), n1 is 1, and Y is NR4, then R4 is not C1-2alkyl or C1-2fluoroalkyl;

    when R3 is the heterocyclic group of sub-formula (aa) and Y is NR4, then R4 is not C(O)NH2, C(O)-C1-2alkyl or C(O)-C1 fluoroalkyl;

    provided that: when R3 is the heterocyclic group of sub-formula (cc), then NHR3 is of sub-formula (L), (m) or (m1), wherein the -NH- connection point of the NHR3 group to the 4-position of the pyrazolopyridine of formula (IA) is underlined:

    and provided that:

    where R3 is optionally substituted C3-8cycloalkyl, then any OH, alkoxy or fluoroalkoxy substituent is at the 3-, 4- or 5-position of the R3 cycloalkyl ring (wherein, in this connection, the 1-position of the R3 cycloalkyl ring is deemed to be the connection point to the -NH- in formula (IA)).


     
    3. A compound or salt as claimed in claim 1 or 2, wherein R2 is a hydrogen atom (H) or methyl.
     
    4. A compound or salt as claimed in claim 1, 2 or 3, wherein R1 is C2-3alkyl, C2fluoroalkyl or -(CH2)2OH.
     
    5. A compound or salt as claimed in any preceding claim, wherein R1 is ethyl, n-propyl, C2fluoroalkyl or -CH2CH2OH.
     
    6. A compound or salt as claimed in any preceding claim, wherein R1 is ethyl.
     
    7. A compound or salt as claimed in any preceding claim, wherein in R3 there is one substituent or no substituent.
     
    8. A compound or salt as claimed in any preceding claim, wherein R3 is optionally substituted C3-8cycloalkyl or the optionally substituted heterocyclic group of sub-formula (aa), (bb) or (cc).
     
    9. A compound or salt as claimed in any preceding claim, wherein, where R3 is the heterocyclic group of sub-formula (aa), (bb) or (cc), then Y is O.
     
    10. A compound or salt as claimed in any preceding claim, wherein where R3 is the heterocyclic group of sub-formula (aa), (bb) or (cc), then R3 is the heterocyclic group of sub-formula (bb) and n1 is 1.
     
    11. A compound or salt as claimed in any preceding claim, wherein, in R3, the heterocyclic group of sub-formula (aa), (bb) or (cc) is unsubstituted (wherein, where Y is NR4, R4 is not classified as a substituent).
     
    12. A compound or salt as claimed in any preceding claim, wherein NHR3 or NR3R3a is of sub-formula (a), (al), (b), (c), (c 1), (c 3), (c 4), (c 5), (d), (e), (f), (g), (g1), (g2), (g3), (g4), (h), (i), (j), (k), (kl), (L), (m), (m1), (m2), (m3), (m5), (n), (o), (o1), (o2), (o3), (o4), (p), (p2), (p3), (p5), (p7) or (q), wherein the -NH- connection point of the NHR3 or NR3R3a group to the 4-position of the pyrazolopyridine of formula (I) or (IA) is underlined:














     
    13. A compound or salt as claimed in claim 12, wherein NHR3 or NR3R3a is of sub-formula (h):


     
    14. A compound or salt as claimed in claim 13, wherein R1 is ethyl.
     
    15. A compound or salt as claimed in any preceding claim, wherein Het is of sub-formula (i), (ii) or (v).
     
    16. A compound or salt as claimed in claim 15, wherein Het is of sub-formula (ia), (ib), (ic), (id), (ie), (if), (ig), (va), (vb) or (iia):






     
    17. A compound or salt as claimed in claim 16, wherein Het is of sub-formula (ia), (ic) or (id).
     
    18. A compound which is:

    N-Cyclopentyl-1-elhyl-5-(5-melhyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclopentyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclopentyl-1-elhyl-5.(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclopentyl-1-ethyl-5-(5-methyl-1,3,4-thiadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclopentyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-thiadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclopentyl-1-ethyl-5-(5-isopropyl-1,3,4-thiadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclohexyl-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclohexyl-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(S-methyl-1,3,4-oxadiazol-2-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-N-cyclohexyl-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-N-cyclopentyl-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-Cyclohexyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amine,

    I-Ethyl-5-(3-methyl-1,2,4-oxadiazol-5-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[3-(methoxymethyl)-1,2,4-oxadiazol-5-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-{3-[(Dimethylamino)methyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[3-(morpholin-4-ylmethyl)1,2,4-oxadiazol-5-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-Cyclopropyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(3-methyloxetan-3-yl)-1,3,4-oxadiazol-2-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(4-methylpiperazin-1-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-isopropyl-1,3,4-oxadiazole-2-carboxam ide,

    4-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-1-methylpyrrolidin-2-one,

    1-Ethyl-N-tetrahydro-2H-pyran-4-yl-5-(5-tetrahydro-2H-pyran-4-yl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(morpholin-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-am ine,

    5-[5-(Tert-butoxymethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine, or

    methyl 2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxylate;

    or a salt thereof.


     
    19. A compound which is:

    Methyl 2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-4,5-dihydro-1,3-oxazole-4-carboxylate,

    1-Ethyl-5-(4-methyl-4,5-dihydro-1,3-oxazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-(n-Propyl)-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(tetrahydrofuran-2-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(dimethylamino)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(5-methyl-1,2,4-triazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-(3-methyl-1,2,4-oxadiazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine, or

    N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-[3-(morpholin-4-ylmethyl)-1,2,4-oxadiazol-5-yl]-I H-pyrazolo[3,4-b]pyridin-4-amine;

    or a salt thereof.


     
    20. A compound which is:

    1-Ethyl-5-[(4R)-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(4S)-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(4S)-4-(phenylmethyl)-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(4R)-4-(phenylmethyl)-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(4S,5R)-5-methyl-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(5R)-5-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[(5S)-5-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(4,4-Dimethyl-4,5-dihydro-1,3-oxazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxylic acid,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(1-methylethyl)-1,3-oxazole-4-carboxamide,

    1-Ethyl-5-[4-(4-morpholinylcarbonyl)-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    trans-4-{[1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-yl]amino}cyclohexanol,

    1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-3-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    4-{[1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-yl]amino}cyclohexanone,

    5-[5-(1,1-Dimethylethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-6-methyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-6-methyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-Cyclobutyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-2-pyrrolidinone,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)acetamide,

    1-Ethyl-5-[5-(1-methyl-2-piperidinyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(4-methyl-1,2,5-oxadiazol-3-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    3-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}cyclopentanone,

    1-Ethyl-5-[5-(tetrahydro-3-furanyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    (4S)-4-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-1,3-thiazolidin-2-one,

    5-[5-(2,2-Dimethylcyclopropyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)-N-methylacetamide,

    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(1-methylcyclobutyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(3-methyl-5-isoxazolyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(1-methyl-1H-pyrazol-5-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-[5-(1-Acetyl-4-piperidinyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[(4-methyl-1-piperazinyl)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[3-(4-fluorophenyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine, or

    1-Ethyl-5-{3-[2-oxo-2-(1-pyrrolidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine;

    or a salt thereof.


     
    21. A compound which is:

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-phenylacetamide,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(1-phenylethyl)acetamide,

    1-Ethyl-5-{3-[2-oxo-2-(1-piperidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(phenylmethyl)acetamide,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N,N-dimethylacetamide,

    N-Ethyl-2-{5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}acetamide,

    1-Ethyl-5-{3-[1-(4-morpholinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin4-amine,

    5-[3-(Cyclohexylmethyl)-1,2,4-oxadiazol-5-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[2-oxo-2-(1-piperidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-ethyl-5-{3-[2-(4-methyl-1-piperazinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(1H-1,2,3-triazol-1-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-{5-[(2,4-Dimethyl-1,3-thiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(2-furanylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(3-isoxazolylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-ethyl-5-(5-{[4-(methyloxy)phenyl]methyl}-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(1H-tetrazol-1-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(5-isothiazolylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(3-methyl-5-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-{[4-(Dimethylamino)phenyl]methyl}-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (1:1),

    1-Ethyl-5-(5-[(2-methyl-1,3-thiazol-4-yl)methyl]-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-[1-({5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadincl-2-yl}methyl)cyclopentyl]-N-methylacetamide,

    N-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)cyclopropanecarboxamide,

    1-Ethyl-5-{5-[(5-methyl-3-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(5-methyl-3-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[2-(4-methyl-1,3-thiazol-5-yl)ethyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-{5-[(3,5-Dimethyl-4-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-(1-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}ethyl)acetamide,

    5-{5-[(1-acetyl-4-piperidinyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{5-[(4-methylphenyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[5-(4-methylphenyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-[5-(3,4-Dimethylphenyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-[5-(2,4-Dimethylphenyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-{5-[(4-Bromophenyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(phenylmethyl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{[4-(methytoxy)phenyl]methyl}-1,3-oxazote-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(2-methylphenyl)methyl]-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(4-methylphenyl)methyl]-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(3-methylphenyl)methyl]-1,3-oxazole-4-carboxamide,

    N-[(4-Chlorophenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    N-[(2,3-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    N-[(3,5-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    N-[(3,4-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(1-phenylethyl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{(1R)-1-[4-(methyloxy)phenyl]ethyl}-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(1R)-1-phenylpropyl]-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(4-methylphenyl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-({4-[(methylsulfonyl)amino]phenyl}methyl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{[4-(methylsulfonyl)phenyl]methyl}-1,3-oxazole-4-carboxamide,

    N-(1-Acetyl-4-pipendinyl)-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(tetrahydro-2-furanylmethyl)-1,3-oxazole-4-carboxamide,

    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[2-(4-methyl-1-piperazinyl)ethyl]-1,3-oxazole-4-carboxamide,

    N-[1-(Aminomethyl)cyclohexyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-methyl-1,3-oxazole-4-carboxamide,

    N-(2,6-Dimethylphenyl)-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    N- [4-(Aminocarbonyl)phenyl]methyl}-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazole-4-carboxamide,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(tetrahydro-2H-pxan-4-yl)acetamide,

    5-{3-[2-(2,6-Dimethyl-4-morpholinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[2-(4-methyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl}-1,2,4-oxadiazol-3-yl}-N-[1-methyl-2-(methyloxy)ethyl]acetamide,

    5-{3-[2-(3,5-Dimethyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[2-(3-methyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-3-pyridinylacetamide,

    6-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-2-piperidinone,

    1-Ethyl-5-{5-[(3-methyl-1H-1,2,4-triazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)acetamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)benzamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-phenylacetamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadincl-3-yl}ethyl)-2-methylpropanamide,

    N-{5-[1-Ethyl-4-(tetrahydro-2H-yran-4-ylamino)-1H-yrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-3-methylbutanamide,

    N-({5-[1Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)cyclohexanecarboxamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-furancarboxamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)methanesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)benzenesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-1-phenylmethanesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-y1]-1,2,4-oxadiazol-3-yl}methyl)-2-propanesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-1-propanesulfonamide,

    N-({5-[1-Ethyl4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)cyclopropanesulfonamide,

    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylmnino)-1H-pyrazolo[3,4-blpyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-thiophenesulfonamide,

    1-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-pyrrolidinone,

    1-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-piperidinone,

    5-{3-[(1-Acetyl-4-pipefidinyl)methyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(3-{[1-(3-methylbutanoyl)-4-piperidinyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(3-{[1-(methylsulfonyl)-4-piperidinyl]methy}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[1-(phenylsulfonyl)cyclopropyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[3-(phenylmethyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-[3-(1-phenylethyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(3-{[4-(methyloxy)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(3-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(3-{[3-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(3-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[(phenyloxy)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[3-(5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyridin-3-ylmethyl)-1,2,4-oxadiazol-5-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-{3-[(4-phenyl-1-piperazinyl)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(5-ethyl-1,2,4-oxadiazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    5-(5-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-3-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine,

    1-Ethyl-5-(5-{[4-(methyloxy)phenyl]methyl}-1,2,4-oxadiazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyfidin-4-amine, or

    5-(3,8-Dioxa-1-azaspiro[4.5]dec-1-en-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine;

    or a salt thereof.


     
    22. A compound which is:

    1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 14),

    5-(5-Tert-butyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 17),

    1-Ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 23),

    1-Ethyl-5-[5-(3-methyloxetan-3-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 34),

    1-Ethyl-5-{5-[(4-methylpiperazin-1-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-y1)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 35),

    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 38), also named: 1-Ethyl-5-[5-(morpholin-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 39),

    1-Ethyl-5-[5-(tetrahydrofuran-2-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 44),

    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 77), or

    1-Ethyl-5-3-[2-oxo-2-(1-pyrrolidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine (the compound of Example 84);

    or a salt thereof.


     
    23. A compound or salt as claimed in claim 22, which is 1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine

    or a salt thereof.
     
    24. A compound or salt as claimed in claim 18, which is 5-(5-cyclopropyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amine

    or a salt thereof.
     
    25. A compound or salt as claimed in claim 20, which is 1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amine

    or a salt thereof.
     
    26. A compound or salt as claimed in claim 21, which is 5-{5-[(2,4-dimethyl-1,3-thiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine

    or a salt thereof.
     
    27. A compound or salt as claimed in claim 21, which is 5-{5-[(2,4-dimethyl-1,3-thiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine

    or a pharmaceutically acceptable salt thereof.
     
    28. A compound or salt as claimed in claim 21, which is 5-{5-[(2,4-dimethyl-1,3-thiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amine


     
    29. A compound, or pharmaceutically acceptable salt thereof, as claimed in any preceding claim, for use as an active therapeutic substance in a mammal such as a human.
     
    30. A pharmaceutical composition comprising a compound as defined in any of claims 1 to 28, or a pharmaceutically acceptable salt thereof, and one or more pharmaceutically acceptable carriers and/or excipients.
     
    31. A pharmaceutical composition as claimed in claim 30 which is suitable for and/or adapted for oral administration.
     
    32. A pharmaceutical composition as claimed in claim 30 or 31, for the treatment and/or prophylaxis of an inflammatory and/or allergic disease, or of cognitive impairment in a neurological disorder, in a mammal such as a human.
     
    33. The use of a compound as defined in any of claims 1 to 28, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for the treatment and/or prophylaxis of an inflammatory and/or allergic disease, or of cognitive impairment in a neurological disorder, in a mammal such as a human.
     
    34. The use of a compound as defined in any of claims 1 to 28, or a pharmaceutically acceptable salt thereof, in the manufacture of a medicament for the treatment and/or prophylaxis of: asthma, chronic obstructive pulmonary disease (COPD), atopic dermatitis, urticaria, allergic rhinitis, allergic conjunctivitis, vernal conjunctivitis, eosinophilic granuloma, psoriasis, rheumatoid arthritis, septic shock, ulcerative colitis, Crohn's disease, reperfusion injury of the myocardium and brain, chronic glomerulonephritis, endotoxic shock, adult respiratory distress syndrome, multiple sclerosis, cognitive impairment in a neurological disorder, depression, or pain; in a human.
     
    35. A pharmaceutical composition as claimed in claim 30, for use in the treatment and/or prophylaxis of: asthma, chronic obstructive pulmonary disease (COPD), atopic dermatitis, urticaria, allergic rhinitis, allergic conjunctivitis, vernal conjunctivitis, eosinophilic granuloma, psoriasis, rheumatoid arthritis, septic shock, ulcerative colitis, Crohn's disease, reperfusion injury of the myocardium and brain, chronic glomerulonephritis, endotoxic shock, adult respiratory distress syndrome, multiple sclerosis, cognitive impairment in a neurological disorder, depression, or pain; in a human.
     
    36. A compound or pharmaceutically acceptable salt thereof, as claimed in any of claims 1 to 28, for the treatment and/or prophylaxis of depression in a human.
     
    37. A compound or pharmaceutically acceptable salt thereof, as claimed in any of claims 1 to 28, for the treatment and/or prophylaxis of cognitive impairment in a human.
     
    38. A compound or pharmaceutically acceptable salt thereof, as claimed in claim 37,
    wherein the cognitive impairment is in a neurological disorder.
     
    39. A compound or pharmaceutically acceptable salt thereof, as claimed in claim 38,
    wherein the neurological disorder is Alzheimer's disease.
     
    40. The use of a compound or a pharmaceutically acceptable salt thereof, as claimed in any of claims 1 to 28, in the manufacture of a medicament for the treatment and/or prophylaxis of depression in a human.
     
    41. The use of a compound or a pharmaceutically acceptable salt thereof, as claimed in any of claims 1 to 28, in the manufacture of a medicament for the treatment and/or prophylaxis of cognitive impairment in a human.
     
    42. The use of a compound or a pharmaceutically acceptable salt thereof, as claimed in claim 41, wherein the cognitive impairment is in a neurological disorder.
     
    43. The use of a compound or a pharmaceutically acceptable salt thereof, as claimed in claim 42, wherein the neurological disorder is Alzheimer's disease.
     


    Ansprüche

    1. Verbindung der Formel (I) oder ein Salz davon:

    wobei:

    R1 C1-3-Alkyl, C1-2-Fluoralkyl oder-(CH2)2OH ist;

    R2 ein Wasserstoffatom (H), Methyl oder C1-Fluoralkyl ist;

    R3 gegebenenfalls substituiertes C3-8-Cycloalkyl oder ein gegebenenfalls substituierter heterocyclischer Rest der Unterformel (aa), (bb) oder (cc) ist:

    wobei n1 und n2 unabhängig 1 oder 2 sind; und Y O, S, SO2 oder NR4 ist; wobei R4 ein Wasserstoffatom (H), C1-2-Alkyl, C1-2-Fluoralkyl, C(O)NH2, C(O)-C1-2-Alkyl oder C(O)-C1-Fluoralkyl ist;
    wobei in R3 das C3-8-Cycloalkyl gegebenenfalls mit einem oder zwei Substituenten substituiert ist, welche unabhängig Oxo (=O); OH; C1-Alkoxy; C1-Fluoralkoxy; NHR21,
    wobei R21 ein Wasserstoffatom (H) ist; C1-2Alkyl; C1-Fluoralkyl; -CH2OH; -CH2NHR22, wobei R22 H ist; -C(O)OR23, wobei R23 H ist; -C(O)NHR24, wobei R24 H ist; -C(O)R25, wobei R25 Methyl ist; Fluor; Hydroxyimino (=N-OH); oder (C1-2-Alkoxy)imino (=N-OR26, wobei R26 C1-2-Alkyl ist) sind; und wobei jeder OH-, Alkoxy-, Fluoralkoxy-, oder NHR21-Substituent nicht an dem R3-Ring-Kohlenstoffatom, welches am -NH-Rest der Formel (I) liegt (gebunden ist), substituiert ist;
    und wobei in R3 der heterocyclische Rest der Unterformel (aa), (bb) oder (cc) gegebenenfalls mit einem oder zwei Substituenten, welche Oxo (=O) sind, substituiert ist;
    und R3a ein Wasserstoffatom (H) ist;
    und wobei Het aus der Unterformel (i), (ii), (iii), (iv) oder (v) besteht:

    wobei:

    W1, W2, W4 und W5 N sind; und W3 NRW ist;

    X1, X3 und X4 N oder CRX sind; X2 O, S oder NRX ist; und X5 CRX1RX2 oder CRX3RX4 ist;

    Y1, Y2 und Y3 CRY oder N sind; Y4 O, S oder NRY ist; und Y5 CRY1RY2 ist;

    Z1 und Z5 O, S oder NRZ sind; und Z2, Z3 und Z4 N oder CRZ sind;
    wobei

    RW ein Wasserstoffatom (H) ist;

    RX, RX2, RY und RY2 jeweils unabhängig sind:

    ein Wasserstoffatom (H);

    C1-8-Alkyl;

    C3-6-Cycloalkyl, gegebenenfalls substituiert mit einem oder zwei C1-2-Alkylresten und/oder einem Oxo(=O)-Rest;

    -(CH2)n2a-C3-6-Cycloalkyl, gegebenenfalls substituiert, in der -(CH2)n2a-Einheit oder in der C3-6-Cycloalkyleinheit, mit einem C1-2-Alkylrest, oder gegebenenfalls substituiert in der C3-6-Cycloalkyleinheit mit einem -CH2C(O)NHC1-2-Alkylrest, wobei n2a 1, 2 oder 3 ist;

    -(CH2)n3-S(O)2-R5, -CH(C1-2-Alkyl)-S(O)2-R5, -CMe2-S(O)2-R5 oder C3-5-Cycloalkyl, substituiert an dem verbindenden Kohlenstoffatom mit -S(O)2-R5, wobei n3 1 oder 2 ist; und R5 C1-4-Alkyl, -NR15R16, Phenyl, am Kohlenstoff verbundenes Pyridinyl oder Benzyl ist (wobei das Phenyl und Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem oder zwei Substituenten substituiert sind, welche unabhängig Fluor, Chlor, C1-2-Alkyl, C1-Fluoralkyl, C1-2-Alkoxy, C1-Fluoralkoxy oder OH sind, und wobei das Pyridinyl gegebenenfalls mit einem Methyl, Methoxy oder OH (einschließlich jeglichem Tautomer davon) substituiert ist);

    wobei R15 H, C1-4-Alkyl, Phenyl, Benzyl (wobei das Phenyl und Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem oder zwei Substituenten substituiert sind, welche unabhängig Fluor, Chlor, C1-2-Alkyl, C1-Fluoralkyl, C1-2-Alkoxy oder C1-Fluoralkoxy sind), CH(Me)Ph oder am Kohlenstoff verbundenes Pyridinyl, gegebenenfalls substituiert mit einem Methyl, Methoxy oder OH (einschließlich jeglichem Tautomer davon) ist; und R16 H oder C1-2-Alkyl ist;
    oder wobei R15 und R16 zusammen -(CH2)n3a-X3a-(CH2)n3b- sind, wobei n3a und n3b unabhängig 2 oder 3 sind und X3a eine Bindung, -CH2-, O oder NR8a ist, wobei R8a H oder C1-2-Alkyl, Acetyl, -S(O)2Me oder Phenyl ist, und wobei der durch NR15R16 gebildete Ring gegebenenfalls an einem Ringkohlenstoffatom mit einem oder zwei Substituenten substituiert ist, welche unabhängig Methyl oder Oxo (=O) sind;

    -(CH2)n4-NR6R7, -CH(C1-2-Alkyl)-NR6R7, -CMe2-NR6R7 oder C3-5-Cycloalkyl, substituiert am verbindenden Kohlenstoffatom mit -NR6R7, wobei n4 0, 1, 2 oder 3 ist; und R6 und R7 unabhängig H, C1-6-Alkyl, C3-6-Cycloalkyl, -CH2-C3-6-Cycloalkyl,

    -C(O)R17, -S(O)2R18, Phenyl, Benzyl (wobei das Phenyl und Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem oder zwei Substituenten substituiert sind, welche unabhängig Fluor, Chlor, C1-2-Alkyl, C1-Fluoralkyl, C1-2-Alkoxy oder C1-Fluoralkoxy sind), oder am Kohlenstoff verbundenes Pyridinyl, gegebenenfalls substituiert mit einem Methyl, Methoxy oder OH (einschließlich jeglichem Tautomer davon) sind;

    und wobei R17 und R18 unabhängig C1-6-Alkyl, C3-6-Cycloalkyl, ein gegebenenfalls substituiertes 5-gliedriges Heteroaryl, welches Furyl (Furanyl) oder 1,3-Oxazolyl oder Isoxazolyl oder Oxadiazolyl oder Thienyl oder 1,3-Thiazolyl oder Isothiazolyl oder Pyrrolyl oder Imidazolyl oder Pyrazolyl (alle unabhängig gegebenenfalls substituiert mit einem Oxo und/oder einem oder zwei Methyl), oder Phenyl oder Benzyl (wobei das Phenyl und Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem oder zwei Substituenten substituiert sind, welche unabhängig Fluor, Chlor, C1-2-Alkyl, C1- Fluoralkyl, C1-2-Alkoxy, C1-Fluoralkoxy oder OH sind) ist, oder am Kohlenstoff verbundenes Pyridinyl, gegebenenfalls substituiert mit einem Methyl, Methoxy oder OH (einschließlich jeglichem Tautomer davon) sind;
    oder R6 und R7 zusammen -(CH2)n5-X5-(CH2)n6- sind, wobei n5 und n6 unabhängig 2 oder 3 sind und X5 eine Bindung, -CH2-, O oder NR8 ist, wobei R8 H, C1-2-Alkyl, Acetyl, -S(O)2Me oder Phenyl ist, und wobei der durch NR6R7 gebildete Ring gegebenenfalls an einem Ringkohlenstoffatom mit einem oder zwei Substituenten substituiert ist, welche unabhängig Methyl oder Oxo (=O) sind;

    -(CH2)n7-O-R9; wobei n7 0, 1, 2 oder 3 ist und R9 H, C1-6-Allcyl, C3-6-Cycloalkyl, -CH2-C3-6-Cycloalkyl, -C(O)R17, Phenyl oder Benzyl ist (wobei das Phenyl und Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem oder zwei Substituenten substituiert sind, welche Fluor, Chlor, C1-2-Alkyl, C1-Fluoralkyl, C1-2-Alkoxy oder C1- Fluoralkoxy sind); wobei n7 nur dann 0 ist, wenn das -(CH2)n7-O-R9 an ein Kohlenstoffatom im Het-Ring gebunden ist; und wobei n7 nicht 0 ist, wenn Het aus der Unterformel (v) besteht (d.h. n7 ist nicht 0 für RX2 und für RY2);

    -CH2)n11-C(O)-NR10R11, -CH(C1-2-Alkyl)-C(O)-NR10R11, -CMe2-C(O)-NR10R11 oder C3-5-Cycloalkyl, substituiert am verbindenden Kohlenstoffatom mit -C(O)-NR10R11,

    wobei n11 0, 1 oder 2 ist;
    und wobei R10 und R11 unabhängig H; C1-6-Alkyl; C1-4-Fluoralkyl; C2-4-Alkyl, substituiert mit einem OH oder -OC1-2-Alkyl, aber nicht am Verbindungspunkt; C3-6-Cycloalkyl, gegebenenfalls substituiert mit einem oder zwei Methylgruppen;

    -CH2-C3-6-Cycloalkyl, gegebenenfalls substituiert mit einer Methylgruppe, einem NH2-oder NHMe-Rest; -(CH2)n17-Het2; am Kohlenstoff verbundenes Pyridinyl, gegebenenfalls substituiert mit einem Methyl, Methoxy oder OH (einschließlich jeglichem Tautomer davon); Phenyl; Benzyl; oder -CH(C1-2-Alkyl)Ph sind [wobei das Phenyl, Benzyl und - CH(C1-2-Alkyl)Ph unabhängig gegebenenfalls am aromatischen Ring mit einem oder zwei Substituenten, welche unabhängig Fluor, Chlor, C1-2-Alkyl, C1-Fluoralkyl, C1-2-Alkoxy, C1-Fluoralkoxy, OH, -NR10aR10b (wobei R10a H oder C1-2-Akyl ist und R10b H, C1-2-Alkyl, -C(O)-C1-2-Alkyl oder -S(O)2-C1-2-Alyl ist), -C(O)-NR10cR10d (wobei R10c und R10d unabhängig H oder C1-2-Alkyl sind) oder -S(O)2R10c: (wobei R10e C1-2-Alkyl, NH2, NHMe oder NMe2 ist) sind, substituiert sind];

    wobei n17 0, 1 oder 2 ist und wobei Het2 ein 4-, 5- oder 6-gliedriger gestättigter heterocyclischer Ring ist, welcher ein O- oder S-Ringatom oder ein NR27-Ringrest enthält, wobei R27 H, C1-2-Akyl, -C(O)Me oder -S(O)2Me ist, wobei der Het2-Ring gegebenenfalls an einem Ringkohlenstoffatom mit einem oder zwei Substituenten substituiert ist, welche unabhängig Methyl oder Oxo (=O) sind;
    und wobei, wenn n17 2 ist, dann der Het2-Ring gegebenenfalls ein zusätzliches Ring-N-Atom an der Het2-Ringposition enthalten kann, welche an die -(CH2)n17-Einheit gebunden ist, mit der Maßgabe, dass wenn Het2 ein O- oder S- oder ein NR27-Ringatom/- rest und ein zusätzliches N-Ringatom enthält, dann das/der O/S/NR27-Ringatom/-rest und das eine zusätzliche N-Ringatom nicht direkt aneinander gebunden sind und durch mehr als ein Kohlenstoffatom getrennt sind;
    oder R10 und R11 zusammen -(CH2)n8-X6-(CH2)n9- sind, wobei n8 und n9 unabhängig 2 oder 3 sind und X6 eine Bindung, -CH2-, O oder NR12 ist, wobei R12 H, C1-2-Alkyl, Acetyl, -S(O)2Me oder Phenyl ist, und wobei der durch NR10R11 gebildete Ring gegebenenfalls an einem Ringkohlenstoffatom mit einem oder zwei Substituenten substituiert ist, welche unabhängig Methyl oder Oxo (=O) sind;

    -(CHn)12-C(O)-OR13, wobei n12 0, 1 oder 2 ist; und wobei R13 H, C1-6-Alkyl, C3-6-Cycloalkyl, -CH2-C3-6-Cycloalkyl, Phenyl oder Benzyl ist (wobei das Phenyl und Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem oder zwei Substituenten, welche (unabhängig) Fluor, Chlor, C1-2-Alkyl, C1-Fluoralkyl, C1-2-Alkoxy oder C1-Fluoralkoxy sind, substituiert sind);

    -(CH2)n13-C(O)-R13a, wobei n13 0, 1 oder 2 ist; und wobei R13a ein Wasserstoffatom (H), C1-6-Alkyl, C1-2-Fluoralkyl, C3-6-Cycloalkyl, -CH2-C3-6-Cycloalkyl, Benzyl oder Phenyl ist, wobei das Phenyl und Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem oder zwei Substituenten, welche (unabhängig) Fluor, Chlor, C1-2-Alkyl, C1-Fluoralkyl, C1-2-Alkoxy oder C1-Fluoralkoxy sind, substituiert sind,

    -(CH2)n14-Het1, -CH(C1-2-Alkyl)-Het1, -CMe2-Het1 oder C3-5-Cycloalkyl, substituiert am verbindenden Kohlenstoffatom mit Het1, wobei n14 0, 1 oder 2 ist und wobei Het1 ein 4-, 5-, 6- oder 7-gliedriger gesättigter heterocyclischer Ring ist;

    wobei der heterocyclische Ring Het1 ein O- oder S-Ringatom und/oder ein NR14-Ringrest enthält, wobei R14 H, C1-4-Alkyl, C3-6-Cycloalkyl, Benzyl, Phenyl,

    -C(O)R19 oder -S(O)2R19 ist;

    wobei R19, unabhängig von jedem anderen R19, C1-6-Alkyl, C3-6-Cycloalkyl, Thienyl, Furyl (Furanyl) oder Phenyl oder Benzyl ist, wobei das Phenyl und Benzyl unabhängig gegebenenfalls mit einem oder zwei Substituenten, welche (unabhängig) Fluor, Methyl oder Methoxy sind, substituiert sind;
    und wobei der heterocyclische Ring Het1 gegebenenfalls substituiert ist (an einer Position oder an Positionen, welche von jeder Position NR14 verschieden ist/sind) mit einem oder zwei Oxo (=O) und/oder einem C1-4-Alkylsubstituenten;
    mit der Maßgabe, dass wenn der heterocyclische Ring Het1 ein O- oder S-Ringatom und ein NR14-Ringrest enthält, dann: (a) das O/S-Ringatom und der NR14-Ringrest nicht direkt aneinander gebunden sind, und (b) das O/S-Ringatom und der NR14-Ringrest durch mehr als ein Kohlenstoffatom getrennt sind, es sei denn, dass Het1 eine -NR14-C(O)-O-oder NR14-C(O)-S-Einheit als Teil des Rings enthält; oder

    -(CH2)n10-Ar, -CH(C1-2-Alkyl)-Ar, -CMe2-Ar oder C3-5-Cycloalkyl, substituiert am verbindenden Kohlenstoffatom mit Ar, wobei n10 0, 1 oder 2 ist und

    (i) Ar Phenyl ist, gegebenenfalls substituiert mit einem oder zwei Substituenten, welche unabhängig Fluor, Chlor, Brom, C1-2-Alkyl, C1-2-Fluorakyl, C1-2-Alkoxy, C1-2-Fluoralkoxy, OH, -NR11aR11b sind (wobei R11a H oder C1-2-Alkyl ist und R11b H, C1-2-Alkyl, -C(O)-C1-2-Alkyl oder -S(O)2-C1-2-Alkyl ist), Cyano, -C(O)-NR11cR11d (wobei R11c und R11d unabhängig H oder C1-2-Alkyl sind), -C(O)-OR11e, wobei R11e H oder C1-2-Alkyl ist, oder -S(O)2-R11f (wobei R11f C1-2-Alkyl, NH2, NHMe oder NMe2 ist); oder das Phenyl Ar gegebenenfalls an zwei benachbarten Ar-Ringatomen mit den zwei Enden einer Kette substituiert ist, welche -(CH2)4-, -(CH2)3- oder -CH=CH-CH=CH- ist; oder

    (ii) Ar ein gegebenenfalls substituierter 5- oder 6-gliedriger heterocyclischer aromatischer Ring ist, welcher 1, 2, 3 oder 4 Heteroatome enthält, ausgewählt aus O, N oder S; und

    wobei, wenn der heterocyclische aromatische Ring Ar 2, 3 oder 4 Heteroatome enthält, eines ausgewählt ist aus O, N und S und das/die verbleibende(n) Heteroatom(e) N ist/sind; und wobei der heterocyclische aromatische Ring Ar gegebenenfalls mit einem oder zwei Resten, welche unabhängig C1-4-Alkyl oder OH (einschließlich jedem Ketotautomer eines mit OH substituierten Rings) sind, substituiert ist, oder der heterocyclische aromatische Ring Ar gegebenenfalls mit zwei benachbarten Ar-Ringatomen mit den zwei Enden einer Kette substituiert ist, welche -(CH2)4-, -(CH2)3-oder -CH=CH-CH=CH- ist;

    RX1 und RY1 unabhängig ein Wasserstoffatom (H), C1-2-Alkyl oder C1-Fluoralkyl sind;

    RX3 und RX4 zusammen -(CH2)n15-X7-(CH2)n16- sind, wobei n15 und n16 unabhängig 1 oder 2 sind und X7 eine Bindung, -CH2-, O oder NRX5 ist, wobei RX5 H, C1-2-Alkyl, Acetyl oder -S(O)2Me ist; und

    RZ ein Wasserstoffatom (H) ist;

    mit der Maßgabe, dass

    wenn R3 der heterocyclische Rest der Unterformel (bb) ist, n1 1 ist und Y NR4 ist, dann R4 nicht C1-2-Alkyl oder C1-2-Fluoralkyl ist;

    wenn R3 der heterocyclische Rest der Unterformel (aa) ist und Y NR4 ist, dann R4 nicht C(O)NH2, C(O)-C1-2-Alkyl oder C(O)-C1-Fluoralkyl ist;

    mit der Maßgabe, dass: wenn R3 der heterocyclische Rest der Unterformel (cc) ist, dann NR3R3a aus der Unterformel (L), (m), (m1) oder (m2) besteht, wobei der -NH-Verbindungspunkt des NR3R3a-Rests an die Position 4 des Pyrazolopyridins der Formel

    (I) unterstrichen ist:

    und mit der Maßgabe, dass:

    wenn R3 gegebenenfalls substituiertes C3-8-Cycloalkyl ist, dann sich jeder Substituent OH, Alkoxy, Fluoralkoxy, -CH2OH, -CH2NHR22, -C(O)OR23, -C(O)NHR24, -C(O)R25 oder Fluor an Position 3, 4 oder 5 des R3-Cyclalkylrings befindet (wobei, in diesem Zusammenhang, die Position 1 des R3-Cycloalkylrings der Verbindungspunkt an das -NH- in Formel (I) sein muss).


     
    2. Verbindung oder Salz gemäß Anspruch 1, welche(s) eine Verbindung der Formel (IA) oder ein Salz davon ist:

    wobei:

    R1 C1-3-Alkyl, C1-2-Fluoralkyl oder -(CH2)2OH ist,

    R2 ein Wasserstoffatom (H), Methyl oder C1-Fluoralkyl ist;

    R3 gegebenenfalls substituiertes C3-8-Cycloalkyl oder ein gegebenenfalls substituierter heterocyclischer Rest der Unterformel (aa), (bb) oder (cc) ist:

    wobei n1 und n2 unabhängig 1 oder 2 sind; und Y O, S, SO2 oder NR4 ist; wobei R4 ein Wasserstoffatom (H), C1-2-Alkyl, C1-2-Fluoralkyl, C(O)NH2, C(O)-C1-2-Alkyl oder C(O)-C1-Fluoralkyl ist;
    wobei in R3 das C3-8-Cycloalkyl gegebenenfalls mit einem oder zwei Substituenten substituiert ist, welche Oxo (=O), OH, C1-Alkoxy, C1-Fluoralkoxy oder C1-2-Alkyl ist; und wobei jeder Substituent OH, Alkoxy oder Fluoralkoxy nicht an dem R3-RingKohlenstoffatom, welches am -NH-Rest der Formel (I) liegt (gebunden ist), substituiert ist;
    und wobei in R3 der heterocyclische Rest der Unterformel (aa), (bb) oder (cc) gegebenenfalls mit einem oder zwei Substituenten, welche Oxo (=O) sind, substituiert ist;
    und wobei Het aus der Unterformel (i), (ii), (iii), (iv) oder (v) besteht:

    wobei:

    W1, W2, W4 und W5 N sind; und W3 NRW ist;

    X1, X3 und X4 N oder CRX sind; X2 O, S oder NRX ist; und X5 CRX1RX2 ist;

    Y1, Y2 und Y3 CRY oder N sind; Y4 O, S oder NRY ist; und Y5 CRY1RY2 ist;

    Z1 und Z5 O, S oder NRZ sind und Z2, Z3 und Z4 N oder CRZ sind;

    wobei:

    RW ein Wasserstoffatom (H) ist;

    RX, RX2, RY und RY2 jeweils unabhängig sind:

    ein Wasserstoffatom (H);

    C1-8-Alkyl;

    C3-6-Cycloalkyl, gegebenenfalls substituiert mit einem C1-2-Alkylrest;

    -(CH2)n2a-C3-6-Cycloalkyl, gegebenenfalls substituiert, in der -(CH2)n2a-Einheit oder in der C3-6-Cycloalkyleinheit, mit einem C1-2-Alkylrest, wobei n2a 1, 2 oder 3 ist;

    -(CH2)n3-S(O)2-R5, wobei n3 1 oder 2 ist und R5 C1-3-Alkyl oder NH-C1-2-Alkyl oder Phenyl ist;

    -(CH2)n4-NR6R7, wobei n4 0, 1, 2 oder 3 ist und R6 und R7 unabhängig H, C1-6-Alkyl, C3-6-Cycloalkyl, -CH2-C3-6-Cycloalkyl, -C(O)-C1-2-Akyl, -SO2-C1-2-Alkyl, Phenyl oder Benzyl sind (wobei das Phenyl oder Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem Substituenten substituiert ist, welcher Fluor, Chlor, C1-2-Alkyl, C1-Fluoralkyl, C1-2-Alkoxy oder C1-Fluoralkoxy ist); oder R6 und R7 zusammen

    -(CH2)n5-X5-(CH2)n6- sind, wobei n5 und nb unabhängig 2 oder 3 sind und X5 eine Bindung, -CH2-, O oder NR8 ist, wobei R8 H oder C1-2-Alkyl ist;

    -(CH2)n7-O-R9; wobei n7 0, 1, 2 oder 3 ist und R9 H oder C1-6-Alkyl ist; wobei n7 nur dann 0 ist, wenn das -(CH2)n7-O-R9 an ein Kohlenstoffatom im Het-Ring gebunden ist; und wobei n7 nicht 0 ist, wenn Het aus der Unterformel (v) besteht (d.h. n7 ist nicht 0 für RX2 und für RY2);

    -C(O)-NR10R11, wobei R10 und R11 unabhängig H, C1-6-Alkyl, C3-6-Cycloalkyl,

    -CH2-C3-6-Cycloalkyl, Phenyl oder Benzyl sind (wobei das Phenyl oder Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem Substituenten substituiert sind, welcher Fluor, Chlor, C1-2-Alkyl, C1-Fluorakyl, C1-2-Alkoxy oder C1-Fluoralkoxy ist); oder R10 und R11 zusammen -(CH2)n8-X6-(CH2)n9- sind, wobei n8 und n9 unabhängig 2 oder 3 sind und X6 eine Bindung, -CH2-, O oder NR12 ist, wobei R12 H, oder C1-2-Alkyl ist;

    -C(O)-OR13 wobei R13 H, C1-6-Alkyl, C3-6-Cycloalkyl, -CH2-C3-6-Cycloalkyl, Phenyl oder Benzyl ist (wobei das Phenyl oder Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem Substituenten substituiert sind, welcher Fluor, Chlor, C1-2-Alkyl, C1-Fluoralkyl, C1-2-Alkoxy oder C1-Fluoralkoxy ist);

    -C(O)-R13a, wobei R13a ein Wasserstoffatom (H), C1-6-Alkyl, C1-2-Fluoralkyl, C3-6-Cycloalkyl, -CH2-C3-6-Cycloalkyl, Benzyl oder Phenyl ist; wobei das Phenyl oder Benzyl unabhängig gegebenenfalls am aromatischen Ring mit einem Substituenten substituiert sind, welcher Fluor, Chlor, C1-2-Alkyl, C1-Fluoralkyl, C1-2-Alkoxy oder C1-Fluoralkoxy ist;

    ein 4-, 5-, 6- oder 7-gliedriger gesättigter heterocyclischer Ring, welcher ein O-Ringatom oder einen NR14-Ringrest enthält, wobei R14 H oder C1-4-Alkyl ist, wobei der heterocyclische Ring gegebenenfalls substituiert ist (an einer Position oder an Positionen, welche von jeder Position NR14 verschieden ist/sind) mit einem Oxo (=O) und/oder einem C1-4-Alkylsubstituenten; oder

    -(CH2)n10-Ar, wobei n10 0, 1 oder 2 ist und

    (i) Ar Phenyl ist, gegebenenfalls substituiert mit einem oder zwei Substituenten, welche unabhängig Fluor, Chlor, C1-2-Alkyl, C1-2-Fluoralkyl, C1-2-Alkoxy, C1-2-Fluoralkoxy oder Cyano sind; oder

    (ii) Ar ein gegebenenfalls substituierter 5- oder 6-gliedriger heterocyclischer aromatischer Ring ist, welcher 1, 2 oder 3 Heteroatome enthält, ausgewählt aus O, N oder S; und wobei, wenn der heterocyclische aromatische Ring Ar 2 oder 3 Heteroatome enthält, eines ausgewählt ist aus O, N und S und das/die verbleibende(n) Heteroatom(e) N ist/sind; und wobei der heterocyclische aromatische Ring Ar gegebenenfalls mit einem oder zwei C1-4-Alkylresten substituiert ist;

    RX1 und RY1 unabhängig ein Wasserstoffatom (H), C1-2-Alkyl oder C1-Fluoralkyl sind; und

    RZ ein Wasserstoffatom (H) ist;
    mit der Maßgabe, dass:

    wenn R3 der heterocyclische Rest der Unterformel (bb) ist, n1 1 ist und Y NR4 sind, dann R4 nicht C1-2-Alkyl oder C1-2-Fluoralkyl ist;

    wenn R3 der heterocyclische Rest der Unterformel (aa) ist und Y NR4 ist, dann R4 nicht C(O)NH2, C(O)-C1-2-Alkyl oder C(O)-C1-Fluoralkyl ist;

    mit der Maßgabe, dass: wenn R3 der heterocyclische Rest der Unterformel (cc) ist, dann NHR3 der Unterformel (L), (m), oder (ml) besteht, wobei der -NH-Verbindungspunkt des NHR3-Rests an die Position 4 des Pyrazolopyridins der Formel (IA) unterstrichen ist:

    und mit der Maßgabe, dass:

    wenn R3 gegebenenfalls substituiertes C3-8-Cycloalkyl ist, dann sich jeder Substituent OH, Alkoxy oder Fluoralkoxy an Position 3, 4 oder 5 des R3-Cyclalkylrings befindet (wobei, in diesem Zusammenhang, die Position 1 des R3-Cycloalkylrings der Verbindungspunkt an das -NH- in Formel (IA) sein muss).


     
    3. Verbindung oder Salz gemäß Anspruch 1 oder 2, wobei R2 ein Wasserstoffatom (H) oder Methyl ist.
     
    4. Verbindung oder Salz gemäß Anspruch 1, 2, oder 3, wobei R1 C2-3-Alkyl, C2-Fluoralkyl oder -(CH2)2OH ist.
     
    5. Verbindung oder Salz gemäß einem der vorstehenden Ansprüche, wobei R1 Ethyl, n-Propyl, C2-Fluoralkyl oder -CH2CH2OH ist.
     
    6. Verbindung oder Salz gemäß einem der vorstehenden Ansprüche, wobei R1 Ethyl ist.
     
    7. Verbindung oder Salz gemäß einem der vorstehenden Ansprüche, wobei es in R3 einen Substituenten oder keinen Substituenten gibt.
     
    8. Verbindung oder Salz gemäß einem der vorstehenden Ansprüche, wobei R3 gegebenenfalls substituiertes C3-8-Cycloalkyl oder der gegebenenfalls substituierte heterocyclische Rest der Unterformel (aa), (bb) oder (cc) ist.
     
    9. Verbindung oder Salz gemäß einem der vorstehenden Ansprüche, wobei, wenn R3 der heterocyclische Rest der Unterformel (aa), (bb) oder (cc), dann Y O ist.
     
    10. Verbindung oder Salz gemäß einem der vorstehenden Ansprüche, wobei, wenn R3 der heterocyclische Rest der Unterformel (aa), (bb) oder (cc) ist, dann R3 der heterocyclische Rest der Unterformel (bb) ist und n1 1 ist.
     
    11. Verbindung oder Salz gemäß einem der vorstehenden Ansprüche, wobei in R3 der heterocyclische Rest der Unterformel (aa), (bb) oder (cc) unsubstituiert ist (wobei, wenn Y NR4 ist, R4 nicht als Substituent klassifiziert wird).
     
    12. Verbindung oder Salz gemäß einem der vorstehenden Ansprüche, wobei NHR3 oder NR3R3a aus den Unterformeln (a), (a1), (b), (c), (c 1), (c 3), (c 4), (c 5), (d), (e), (f), (g), (g1), (g2), (g3), (g4), (h), (i), (j), (k), (k1), (L), (m), (m1), (m2), (m3), (m5), (n), (o), (o1), (o2), (o3), (o4), (p), (p2), (p3), (p5), (p7) oder (q) besteht, wobei der -NH-Verbindungspunkt des NHR3- oder NR3R3a-Rests an die Position 4 des Pyrazolpyridins der Formel (I) oder (Ia) unterstrichen ist:














     
    13. Verbindung oder Salz gemäß Anspruch 12, wobei NHR3 oder NR3R3a aus der Unterformel (h) besteht:


     
    14. Verbindung oder Salz gemäß Anspruch 13, wobei R1 Ethyl ist.
     
    15. Verbindung oder Salz gemäß einem der vorstehenden Ansprüche, wobei Het aus der Unterformel (i), (ii) oder (v) besteht.
     
    16. Verbindung oder Salz gemäß Anspruch 15, wobei Het aus der Unterformel (ia), (ib), (ic), (id), (ie), (if), (ig), (va), (vb) oder (iia) besteht:






     
    17. Verbindung oder Salz gemäß Anspruch 16, wobei Het aus der Unterformel (ia), (ic) oder (id) besteht.
     
    18. Verbindung, nämlich
    N-Cyclopentyl-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-Cyclopentyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-Cyclopentyl-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-Cyclopentyl-1-ethyl-5-(5-methyl-1,3,4-thiadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-Cyclopentyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-thiadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-Cyclopentyl-1-ethyl-5-(5-isopropyl-1,3,4-thiadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-Cyclohexyl-1-ethyl-5-(5-isopropyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-Cyclohexyl-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-(5-tert.-Butyl-1,3,4-oxadiazo1-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-(5-tert.-Butyl-1,3,4-oxadiazol-2-yl)-N-cyclohexyl-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-(5-tert.-Butyl-1,3,4-oxadiazol-2-yl)-N-cyclopentyl-1-ethyl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-Cyclohexyl-1-ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-(3-methyl-1,2,4-oxadiazol-5-yl)-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[3-(methoxymethyl)-1,2,4-oxadiazol-5-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-{3-[(Dimethylamino)methyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[3-(morpholin-4-ylmethyl)-1,2,4-oxadiazol-5-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-(5-Cyclopropyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(3-methyloxetan-3-yl)-1,3,4-oxadiazol-2-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyazolo[3,4-b]pyidin-4-amin,
    1-Ethyl-5-{5-[(4-methylpiperazin-1-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-tetrahydro-2H-pyran-4-yl-1H-pxazolo[3,4-b]pyridin-4-amin,
    5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-isopropyl-1,3,4-oxadiazole-2-carboxamid,
    4-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-1-methylpyrrolidin-2-on,
    1-Ethyl-N-teh-ahydro-2H-pyran-4-yl-5-(5-tetrahydro-2H-pyran-4-yl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(morpholin-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-[5-(tert.-Butoxymethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-tetrahydro-2H-pyan-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin, oder
    Methyl-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazol-4-carboxylat;
    oder ein Salz davon.
     
    19. Verbindung, nämlich
    Methyl-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pxidin-5-yl]-4,5-dihydro-1,3-oxazol-4-carboxylat,
    1-Ethyl-5-(4-methyl-4,5-dihydro-1,3-oxazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-(n-Propyl)-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(tetrahydrofuran-2-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(dimethylamino)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-(5-methyl-1,2,4-triazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-(3-methyl-1,2,4-oxadiazol-5-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin, oder
    N-(1-Acetylpiperidin-4-yl)-1-ethyl-5-[3-(morpholin-4-ylmethyl)-1,2,4-oxadiazol-5-yl]-1H-pyrazolo[3,4-b]pyridin-4-amin;
    oder ein Salz davon.
     
    20. Verbindung, nämlich
    1-Ethyl-5-[(4R)-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[(4S)-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(terahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[(4S)-4-(phenylmethyl)-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[(4R)-4-(phenylmethyl)-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[(4S,5R)-5-methyl-4-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tehahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pryridin-4-amin,
    1-Ethyl-5-[(5R)-5-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[(S5)-5-phenyl-4,5-dihydro-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-(4,4-Dimethyl-4,5-dihydro-1,3-oxazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazol-4-carbonsäure,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(1-methylethyl)-1,3-oxazol-4-carboxamid,
    1-Ethyl-5-[4-(4-morpholinylcarbonyl)-1,3-oxazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    trans-4-{[1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-yl]amino}cyclohexanol,
    1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-3-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    4-{[1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-1H-pyrazolo[3,4-b]pyridin-4-yl]amino}cyclohexanon,
    5-[5-(1,1-Dimethylethyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-6-methyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-6-methyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-(5-Cyclobutyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-2-pyrrolidinon,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)acetamid,
    1-Ethyl-5-[5-(1-methyl-2-piperidinyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{5-[(4-methyl-1,2,5-oxadiazol-3-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    3-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}cyclopentanon,
    1-Ethyl-5-[5-(tetrahydro-3-furanyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    (4S)-4-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-1,3-thiazolidin-2-on,
    5-[5-(2,2-Dimethylcyclopropyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)-N-methylacetamid,
    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(1-methylcyclobutyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(3-methyl-5-isoxazolyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(1-methyl-1H-pyrazol-5-yl)-1,3,4-oxadiazo1-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-[5-(1-Acetyl-4-piperidinyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{3-[(4-methyl-1-piperazinyl)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amim
    1-Ethyl-5-[3-(4-fluorophenyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin, oder
    1-Ethyl-5-{3-[2-oxo-2-(1-pyrrolidinyl)ethyl]-1,2,4-oxadiazo1-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin;
    oder ein Salz davon.
     
    21. Verbindung, nämlich
    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-phenylacetamid,
    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(1-phenylethyl)acetamid,
    1-Ethyl-5-{3-[2-oxo-2-(1-piperidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(phenylmethyl)acetamid,
    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N,N-dimethylacetamid,
    N-Ethyl-2-{5-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl} acetamid,
    1-Ethyl-5-{3-[1-(4-morpholinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-[3-(Cyclohexylmethyl)-1,2,4-oxadiazol-5-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{3-[2-oxo-2-(1-piperidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{3-[2-(4-methyl-1-piperazinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(1H-1,2,3-triazol-1-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-{5-[(2,4-Dimethyl-1,3-thiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyreolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(2-furanylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(3-isoxazolylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-(5-{[4-(methyloxy)phenyl]methyl}-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(1H-tetrazol-1-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(5-isothiazolylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{5-[(3-methyl-5-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyadin-4-amin,
    5-(5-{[4-(Dimethylamino)phenyl]methyl}-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin (1:1),
    1-Ethyl-5-{5-[(2-methyl-1,3-thiazol-4-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    2-[1-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)cyclopentyl]-N-methylacetamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}methyl)cyclopropancarboxamid,
    1-Ethyl-5-{5-[(5-methyl-3-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{5-[(5-methyl-3-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{5-[2-(4-methyl-1,3-thiazol-5-yl)ethyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-{5-[(3,5-Dimethyl-4-isoxazolyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-(1-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}ethyl)acetamid,
    5-{5-[(1-acetyl-4-piperidinyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{5-[(4-methylphenyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[5-(4-methylphenyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-[5-(3,4-Dimethylphenyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-[5-(2,4-Dimethylphenyl)-1,3,4-oxadiazol-2-yl]-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-{5-[(4-Bromphenyl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(phenylmethyl)-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{[4-(methyloxy)phenyl]methyl}-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(2-methylphenyl)methyl]-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pxidin-5-yl]-N-[(4-methylphenyl)methyl]-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(3-methylphenyl)methyl]-1,3-oxazol-4-carboxamid,
    N-[(4-Chlorophenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazol-4-carboxamid,
    N-[(2,3-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazol-4-carboxamid,
    N-[(3,5-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazol-4-carboxamid,
    N-[(3,4-Dimethylphenyl)methyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(1-phenylethyl)-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{(1R)-1-[4-(methyloxy)phenyl]ethyl}-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[(1R)-1-phenylpropyl]-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(4-methylphenyl)-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-({4-[(methylsulfonyl)amino]phenyl}methyl)-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-{[4-(methylsulfonyl)phenyl]methyl}-1,3-oxazol-4-carboxamid,
    N-(1-Acetyl-4-piperidinyl)-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-(tetrahydro-2-furanylmethyl)-1,3-oxazol-4-carboxamid,
    2-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-[2-(4-methyl-1-piperazinyl)ethyl]-1,3-oxazol-4-carboxamid,
    N-[1-(Aminomethyl)cyclohexyl]-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-N-methyl-1,3-oxazol-4-carboxamid,
    N-(2,6-Dimethylphenyl)-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazol-4-carboxamid,
    N-{[4-(Aminocarbonyl)phenyl]methyl}-2-[1-ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3-oxazol-4-carboxamid,
    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazo!o[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-(tetrahydro-2H-pyran-4-yl)acetamid,
    5-{3-[2-(2,6-Dimethyl-4-morpholinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{3-[2-(4-methyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-[1-methyl-2-(methyloxy)ethyl]acetamid,
    5-{3-[2-(3,5-Dimethyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{3-[2-(3-methyl-1-piperidinyl)-2-oxoethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    2-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}-N-3-pyridinylacetamid,
    6-{5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylanino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,3,4-oxadiazol-2-yl}-2-piperidinon,
    1-Ethyl-5-{5-[(3-methyl-1H-1,2,4-triazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazo1-3-yl}methyl)acetamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)benzamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-phenylacetamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-methylpropanamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-3-methylbutanamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)cyclohexancarboxamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrnolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-furancarboxamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)methansulfonamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)benzolsulfonamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-1-phenylmethansulfonamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-propansulfonamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-1-propansulfonamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)cyclopropansulfonamid,
    N-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-thiophensulfonamid,
    1-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-pyrrolidinon,
    1-({5-[1-Ethyl-4-(tetrahydro-2H-pyran-4-ylamino)-1H-pyrazolo[3,4-b]pyridin-5-yl]-1,2,4-oxadiazol-3-yl}methyl)-2-piperidinon,
    5-{3-[(1-Acetyl-4-piperidinyl)methyl]-1,2,4-oxadiazol-5-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-(3-{[1-(3-methylbutanoyl)-4-piperidinyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-(3-{[1-(methylsulfonyl)-4-piperidinyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{3-[1-(phenylsulfonyl)cyclopropyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[3-(phenylmethyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-[3-(1-phenylethyl)-1,2,4-oxadiazol-5-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-(3-{[4-(methyloxy)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-(3-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-(3-{[3-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-(3-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-5-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{3-[(phenyloxy)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[3-(5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyridin-3-ylmethyl)-1,2,4-oxadiazol-5-yl]-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-{3-[(4-phenyl-1-piperazinyl)methyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-(5-ethyl-1,2,4-oxadiazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    5-(5-{[4-(Dimethylamino)phenyl]methyl}-1,2,4-oxadiazol-3-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin,
    1-Ethyl-5-(5-{[4-(methyloxy)phenyl]methyl}-1,2,4-oxadiazol-3-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin, oder
    5-(3,8-Dioxa-1-azaspiro[4.5]dec-1-en-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin;
    oder ein Salz davon.
     
    22. Verbindung, nämlich
    1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin (die Verbindung des Beispiels 14),
    5-(5-tert-Butyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyndin-4-amin (die Verbindung des Beispiels 17),
    1-Ethyl-5-{5-[(methylsulfonyl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin (die Verbindung des Beispiels 23),
    1-Ethyl-5-[5-(3-methyloxetan-3-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin (die Verbindung des Beispiels 34),
    1-Ethyl-5-{5-[(4-methylpiperazin-1-yl)methyl]-1,3,4-oxadiazol-2-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin (die Verbindung des Beispiels 35),
    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-yl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amin (die Verbindung des Beispiels 38),
    auch bezeichnet als: 1-Ethyl-5-[5-(morpholin-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin (die Verbindung des Beispiels 39),
    1-Ethyl-5-[5-(tetrahydrofüran-2-yl)-1,3,4-oxadiazol-2-yl]-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin (die Verbindung des Beispiels 44),
    1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amin (die Verbindung des Beispiels 77), oder
    1-Ethyl-5-{3-[2-oxo-2-(1-pyrrolidinyl)ethyl]-1,2,4-oxadiazol-5-yl}-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin (die Verbindung des Beispiels 84); oder ein Salz davon.
     
    23. Verbindung oder Salz gemäß Anspruch 22, nämlich
    1-Ethyl-5-(5-methyl-1,3,4-oxadiazol-2-yl)-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin

    oder ein Salz davon.
     
    24. Verbindung oder Salz gemäß Anspruch 18, nämlich 5-(5-Cyclopropyl-1,3,4-oxadiazol-2-yl)-1-ethyl-N-tetrahydro-2H-pyran-4-yl-1H-pyrazolo[3,4-b]pyridin-4-amin

    oder ein Salz davon.
     
    25. Verbindung oder Salz gemäß Anspruch 20, nämlich 1-Ethyl-N-(tetrahydro-2H-pyran-4-yl)-5-[5-(tetrahydro-2H-pyran-4-ylmethyl)-1,3,4-oxadiazol-2-yl]-1H-pyrazolo[3,4-b]pyridin-4-amin

    oder ein Salz davon.
     
    26. Verbindung oder Salz gemäß Anspruch 21, nämlich 5-{5-[(2,4-Dimethyl-1,3-thiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin

    ist, oder ein Salz davon.
     
    27. Verbindung oder Salz gemäß Anspruch 21, nämlich 5-{5-[(2,4-Dimethyl-1,3-thiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin

    ist, oder ein pharmazeutisch verträgliches Salz davon.
     
    28. Verbindung oder Salz gemäß Anspruch 21, nämlich
    5-{5-[(2,4-Dimethyl-1,3-thiazol-5-yl)methyl]-1,3,4-oxadiazol-2-yl}-1-ethyl-N-(tetrahydro-2H-pyran-4-yl)-1H-pyrazolo[3,4-b]pyridin-4-amin

    ist.
     
    29. Verbindung oder ein pharmazeutisch verträgliches Salz davon gemäß einem der vorstehenden Ansprüche, zur Verwendung als ein therapeutischer Wirkstoff in einem Säuger, wie einem Menschen.
     
    30. Arzneimittel, umfassend eine Verbindung wie in einem der Ansprüche 1 bis 28 definiert, oder ein pharmazeutisch verträgliches Salz davon, und einen oder mehrere pharmazeutisch verträgliche Träger und/oder Exzipienten.
     
    31. Arzneimittel gemäß Anspruch 30, welches für die orale Verabreichung geeignet ist und/oder für die orale Verabreichung angepasst ist.
     
    32. Arzneimittel gemäß Anspruch 30 oder 31 zur Behandlung und/oder Vorbeugung einer entzündlichen und/oder allergischen Erkrankung oder einer kognitiven Beeinträchtigung bei einer neurologischen Störung in einem Säuger, wie einem Menschen.
     
    33. Verwendung einer Verbindung wie in einem der Ansprüche 1 bis 28 definiert oder eines pharmazeutisch verträglichen Salzes davon, zur Herstellung eines Medikaments zur Behandlung und/oder Vorbeugung einer entzündlichen und/oder allergischen Erkrankung oder einer kognitiven Beeinträchtigung bei einer neurologischen Störung in einem Säuger, wie einem Menschen.
     
    34. Verwendung einer Verbindung wie in einem der Ansprüche 1 bis 28 definiert oder eines pharmazeutisch verträglichen Salzes davon, zur Herstellung eines Medikaments zur Behandlung und/oder Vorbeugung von: Asthma, chronisch-obstruktiver Lungenerkrankung (COPD), atopischer Dermatitis, Nesselausschlag, Heuschnupfen, allergischer Bindehautentzündung, Frühjahrs-Bindehautentzündung, eosinophilem Granulom, Psoriasis, rheumatoider Arthritis, septischem Schock, ulcerativer Colitis, Morbus Crohn, Reperfusionsverletzung der Herzmuskulatur und des Gehirns, chronischer Glomerulonephritis, endotoxischem Schock, Schocklunge, multipler Sklerose, kognitiver Beeinträchtigung bei einer neurologischen Störung, Depression oder Schmerz in einem Menschen.
     
    35. Arzneimittel gemäß Anspruch 30, zur Verwendung zur Behandlung und/oder Vorbeugung von: Asthma, chronisch-obstruktiver Lungenerkrankung (COPD), atopischer Dermatitis, Nesselausschlag, Heuschnupfen, allergischer Bindehautentzündung, Frühjahrs-Bindehautentzündung, eosinophilem Granulom, Psoriasis, rheumatoider Arthritis, septischem Schock, ulcerativer Colitis, Morbus Crohn, Reperfusionsverletzung der Herzmuskulatur und des Gehirns, chronischer Glomerulönephritis, endotoxischem Schock, Schocklunge, multipler Sklerose, kognitiver Beeinträchtigung bei einer neurologischen Störung, Depression oder Schmerz in einem Menschen.
     
    36. Verbindung oder pharmazeutisch verträgliches Salz davon gemäß einem der Ansprüche 1 bis 28 zur Behandlung und/oder Vorbeugung von Depression in einem Menschen.
     
    37. Verbindung oder pharmazeutisch verträgliches Salz davon gemäß einem der Ansprüche 1 bis 28 zur Behandlung und/oder Vorbeugung von kognitiver Beeinträchtigung in einem Menschen.
     
    38. Verbindung oder pharmazeutisch verträgliches Salz davon gemäß Anspruch 37, wobei die kognitive Beeinträchtigung eine neurologische Störung ist.
     
    39. Verbindung oder pharmazeutisch verträgliches Salz davon gemäß Anspruch 38, wobei die neurologische Störung die Alzheimer-Krankheit ist.
     
    40. Verwendung einer Verbindung oder eines pharmazeutisch verträglichen Salzes davon gemäß einem der Ansprüche 1 bis 28, zur Herstellung eines Medikaments zur Behandlung und/oder Vorbeugung von Depression in einem Menschen.
     
    41. Verwendung einer Verbindung oder eines pharmazeutisch verträglichen Salzes davon gemäß einem der Ansprüche 1 bis 28, zur Herstellung eines Medikaments zur Behandlung und/oder Vorbeugung einer kognitiven Beeinträchtigung in einem Menschen.
     
    42. Verwendung einer Verbindung oder eines pharmazeutisch verträglichen Salzes davon gemäß Anspruch 41, wobei die kognitive Beeinträchtigung eine neurologische Störung ist.
     
    43. Verwendung einer Verbindung oder eines pharmazeutisch verträglichen Salzes davon gemäß Anspruch 42, wobei die neurologische Störung die Alzheimer-Krankheit ist.
     


    Revendications

    1. Composé de formule (I) ou un de ses sels :

    formule dans laquelle :

    R1 représente un groupe alkyle en C1 à C3, fluoralkyle en C1 ou C2 ou -(CH2)2OH ;

    R2 représente un atome d'hydrogène (H), un groupe méthyle ou un groupe fluoralkyle en C1 ;

    R3 représente un groupe cycloalkyle en C3 à C8 facultativement substitué, ou un groupe hétérocyclique, facultativement substitué, de sous-formule (aa), (bb) ou (cc) :

    dans laquelle n1 et n2 sont égaux indépendamment à 1 ou 2 ; et Y représente O, S, un groupe SO2 ou NR4 ; dans lequel R4 représente un atome d'hydrogène (H), un groupe alkyle en C1 ou C2, fluoralkyle en C1 ou C2, C(O)NH2, C(O)-(alkyle en C1 ou C2) ou C(O)-(fluoralkyle en C1) ;
    où, dans R3, le groupe cycloalkyle en C3 à C8 est facultativement substitué avec un ou deux substituants consistant indépendamment en des substituants oxo (=O) ; OH ; alkoxy en C1 ; fluoralkoxy en C1 ; NHR21 dans lequel R21 représente un atome d'hydrogène (H) ; un groupe alkyle en C1 ou C2 ; fluoralkyle en C1 ; -CH2OH ; -CH2NHR22 dans lequel R22 représente H ; -C(O)OR23 dans lequel R23 représente H ; -C(O)NHR24 dans lequel R24 représente H ; -C(O)R25 dans lequel R25 représente un groupe méthyle ; fluoro ; hydroxyimino (=N-OH) ; ou (alkoxy en C1 ou C2)-imino (=N-OR26 dans lequel R26 représente un groupe alkyle en C1 ou C2) ; et où n'importe quel substituant OH, alkoxy, fluoralkoxy ou NHR21 n'est pas substitué au niveau de l'atome de carbone du noyau R3 fixé (lié) au groupe -NH- de la formule (I) ;
    et où, dans R3, le groupe hétérocyclique de sous-formule (aa), (bb) ou (cc) est facultativement substitué avec un ou deux substituants consistant en des substituants oxo (=O) ;
    et R3a représente un atome d'hydrogène (H) ;
    et où Het répond à la sous-formule (i), (ii), (iii), (iv) ou (v) :

    dans laquelle :

    W1, W2, W4 et W5 représentent N ; et W3 représente un groupe NRW ;

    X1, X3 et X4 représentent N ou un groupe CRX ; X2 représente 0, S ou un groupe NRX ; et X5 représente un groupe CRX1RX2 ou CRX3RX4 ;

    Y1, Y2 et Y3 représentent un groupe CRY ou N ; Y4 représente O, S ou un groupe NRY ; et Y5 représente un groupe CRY1RY2 ;

    Z1 et Z5 représentent O, S ou un groupe NRZ ; et Z2, Z3 et Z4 représentent N ou un groupe CRZ ;

    dans lesquels :

    RW représente un atome d'hydrogène (H) ;

    RX, RX2, RY et RY2 représentent, indépendamment :

    un atome d'hydrogène (H) ;

    un groupe alkyle en C1 à C8 ;

    un groupe cycloalkyle en C3 à C6 facultativement substitué avec un ou deux groupes alkyle en C1 ou C2 et/ou avec un groupe oxo (=O) ;

    un groupe -(CH2)n21-(cycloalkyle en C3 à C6) facultativement substitué, dans le groupement -(CH2)n2a- ou dans le groupement cycloalkyle en C3 à C6, avec un groupe alkyle en C1 ou C2, ou facultativement substitué dans le groupement cycloalkyle en C3 à C6 avec un groupe -CH2C(O)-NH-(alkyle en C1 ou C2), dans lequel n2a est égal à 1, 2 ou 3 ;

    un groupe -(CH2)n3-S(O)2-R5, -CH-(alkyle en C1 ou C2)-S(O)2-R5, -CMe2-S(O)2-R5, ou cycloalkyle en C3 à C5 substitué au niveau de l'atome de carbone de connexion avec un groupe -S(O)2-R5,

    dans lequel n3 est égal à 1 ou 2 ;

    et R5 représente un groupe alkyle en C1 à C4, -NR15R16, phényle, pyridinyle ou benzyle lié au carbone (dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un ou deux substituants consistant indépendamment en des substituants fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2, fluoralkoxy en C1 ou OH, et dans lequel le groupe pyridinyle est facultativement substitué avec un substituant méthyle, méthoxy ou OH (y compris n'importe laquelle de ses formes tautomères) ;

    dans lequel R15 représente H, un groupe alkyle en C1 à C4, phényle, benzyle (dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un ou deux substituants consistant indépendamment en des substituants fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2 ou fluoralkoxy en C1), CH(Me)Ph, ou un groupe pyridinyle lié au carbone facultativement substitué avec un substituant méthyle, méthoxy ou OH (y compris n'importe laquelle de ses formes tautomères) ;

    et R16 représente H ou un groupe alkyle en C1 ou C2 ;

    ou bien dans lequel R15 et R16, pris conjointement, représentent un groupe - (CH2) n3a-X3a-(CH2)n3b-dans lequel n3a et n3b sont égaux indépendamment à 2 ou 3 et X3a représente une liaison, un groupe -CH2-, 0 ou NR8a dans lequel R8a représente H ou un groupe alkyle en C1 ou C2, acétyle, -S(O)2Me ou phényle, et dans lequel le noyau formé par NR15R16 est facultativement substitué sur un atome de carbone du noyau avec un ou deux substituants consistant indépendamment en des substituants méthyle ou oxo (=O) ;

    -(CH2)n4-NR6R7, -CH-(alkyle en C1 ou C2)-NR6R7, -CMe2-NR6R7 ou cycloalkyle en C3 à C5 substitué au niveau de l'atome de carbone de connexion avec un substituant -NR6R7, dans lequel n4 est égal à 0, 1, 2 ou 3 ;

    et R6 et R7, indépendamment, représentent H, un groupe alkyle en C1 à C6, cycloalkyle en C3 à C6, -CH2-(cycloalkyle en C3 à C6), -C(O)R17, -S(O)2R18, phényle, benzyle (dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un ou deux substituants consistant indépendamment en des substituants fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2 ou fluoralkoxy en C1), ou un groupe pyridinyle lié au carbone facultativement substitué avec un substituant méthyle, méthoxy ou OH (y compris n'importe laquelle de ses formes tautomères) ;

    et dans lequel R17 et R18, indépendamment, représentent un groupe alkyle en C1 à C6, cycloalkyle en C3 à C6, hétéroaryle pentagonal facultativement substitué consistant en un groupe furyle (furanyle) ou 1,3-oxazolyle ou isoxazolyle ou oxadiazolyle ou thiényle ou 1,3-thiazolyle ou isothiazolyle ou pyrrolyle ou imidazolyle ou pyrazolyle (tous indépendamment facultativement substitués avec un substituant oxo et/ou un ou deux substituants méthyle) ou phényle ou benzyle (dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un ou deux substituants consistant indépendamment en des substituants fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2, fluoralkoxy en C1 ou OH), ou un groupe pyridinyle lié au carbone facultativement substitué avec un substituant méthyle, méthoxy ou OH (y compris n'importe laquelle de ses formes tautomères) ;

    ou bien R6 et R7, conjointement, représentent un groupe -(CH2)n5-X5-(CH2)n6- dans lequel n5 et n6 sont indépendamment égaux à 2 ou 3 et X5 représente une liaison, un groupe -CH2-, O ou NR8 dans lequel R8 représente H, un groupe alkyle en C1 ou C2, acétyle, -S(O)2Me ou phényle, et dans lequel le noyau formé par NR6R7 est facultativement substitué sur un atome de carbone du noyau avec un ou deux substituants consistant indépendamment en des substituants méthyle ou oxo (=O) ;

    -(CH2)n7-O-R9 ; dans lequel n7 est égal à 0, 1, 2 ou 3 et R9 représente H, un groupe alkyle en C1 à C6, cycloalkyle en C3 à C6, -CH2-(cycloalkyle en C3 à C6), -C(O)R17, phényle ou benzyle (dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un ou deux substituants fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2 ou fluoralkoxy en C1) ; dans lequel n7 est égal à 0 seulement lorsque le groupe -(CH2)n7-O-R9 est lié à un atome de carbone dans le noyau Het ; et dans lequel n7 n'est pas égal à 0 lorsque Het répond à la sous-formule (v) (c'est-à-dire n7 n'est pas égal à 0 pour RX2 et pour RY2) ;

    -(CH2)n11-C(O)-NR10R11, -CH-(alkyle en C1 ou C2)-C(O)-NR10R11, -CMe2-C(O)-NR10R11, ou cycloalkyle en C3 à C5 substitué au niveau de l'atome de carbone de connexion avec un substituant -C(O)-NR10R11, dans lequel n11 est égal à 0, 1 ou 2 ;

    et dans lequel R10 et R11 représentent indépendamment H ; un groupe alkyle en C1 à C6 ; fluoralkyle en C1 à C4 ; alkyle en C2 à C4 substitué avec un substituant OH ou -O-(alkyle en C1 ou C2) à un point autre qu'au point de connexion ; cycloalkyle en C3 à C6 facultativement substitué avec un ou deux groupes méthyle ; -CH2-(cycloalkyle en C3 à C6) facultativement substitué avec un groupe méthyle, NH2 ou NHMe ; -(CH2)n17-Het2 ; un groupe pyridinyle lié au carbone facultativement substitué avec un substituant méthyle, méthoxy ou OH (y compris n'importe laquelle de ses formes tautomères) ; phényle ; benzyle ; ou -CH- (alkyle en C1 ou C2)Ph [dans lequel les groupes phényle, benzyle et -CH-(alkyle en C1 ou C2) Ph sont indépendamment facultativement substitués sur le noyau aromatique avec un ou deux substituants consistant indépendamment en des substituants : fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2, fluoralkoxy en C1, OH, -NR10a-R10b (dans lequel R10a représente H ou un groupe alkyle en C1 ou C2 et R10b représente H, un groupe alkyle en C1 ou C2, -C(O)-(alkyle en C1 ou C2) ou -S(O)2-(alkyle en C1 ou C2)), -C (O) -NR10cR10d (dans lequel R10c et R10d représentent indépendamment H ou un groupe alkyle en C1 ou C2) ou -S(O)2-R10e (dans lequel R10e représente un groupe alkyle en C1 ou C2, NH2, NHMe ou NMe2)] ;

    où n17 est égal à 0, 1 ou 2 et où Het2 représente un noyau hétérocyclique saturé tétra-, penta- ou hexagonal contenant un atome de O ou S dans le noyau ou un groupe NR27 dans le noyau, dans lequel R27 représente H, un groupe alkyle en C1 ou C2, -C(O)Me ou -S(O)2Me, le noyau Het2 étant facultativement substitué sur un atome de carbone du noyau avec un ou deux substituants consistant indépendamment en des substituants méthyle ou oxo (=O) ;

    et où lorsque n17 est égal à 2, alors le noyau Het2 peut contenir facultativement un atome de N supplémentaire dans le noyau à la position du noyau Het2 liée au groupement -(CH2)n17- ; sous réserve que, lorsque Het2 contient un atome de O ou S ou groupe NR27 dans le noyau et un atome de N supplémentaire dans le noyau, alors l'atome / groupe de O/S/NR27 du noyau et l'atome de N supplémentaire du noyau ne sont pas liés directement l'un à l'autre, et sont séparés par plus d'un atome de carbone ;

    ou bien R10 et R11, conjointement, représentent un groupe -(CH2)n8-X6-(CH2)n9- dans lequel n8 et n9 sont égaux indépendamment à 2 ou 3 et X6 représente une liaison, un groupe -CH2-, O ou un groupe NR12 dans lequel R12 représente H, un groupe alkyle en C1 ou C2, acétyle, -S(O)2Me ou phényle, et dans lequel le noyau formé par NR10R11 est facultativement substitué sur un atome de carbone du noyau avec un ou deux substituants consistant indépendamment en des substituants méthyle ou oxo (=O) ;

    -(CH2)n12-C(O)-OR13 dans lequel n12 est égal à 0, 1 ou 2 ; et dans lequel R13 représente H, un groupe alkyle en C1 à C6, cycloalkyle en C3 à C6, -CH2-(cycloalkyle en C3 à C6), phényle ou benzyle (dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un ou deux substituants (indépendamment) fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2 ou fluoralkoxy en C1) ;

    - (CH2)n13-C(O)-R13a dans lequel n13 est égal à 0, 1 ou 2 ; et dans lequel R13a représente un atome d'hydrogène (H), un groupe alkyle en C1 à C6, fluoralkyle en C1 ou C2, cycloalkyle en C3 à C6, -CH2- (cycloalkyle en C3 à C6), benzyle ou phényle ; dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un ou deux substituants (indépendamment) fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2 ou fluoralkoxy en C1 ;

    -(CH2)n14-Het1, -CH-(alkyle en C1 ou C2)-Het1, -CMe2-Het1, ou cycloalkyle en C3 à C5 substitué au niveau de l'atome de carbone de connexion avec un substituant Het1, dans lequel n14 est égal à 0, 1 ou 2 et dans lequel Het1 représente un noyau hétérocyclique saturé tétra-, penta-, hexa- ou heptagonal ;

    où ledit noyau hétérocyclique Het1 contient un atome de O ou S dans le noyau et/ou un groupe NR14 dans le noyau, dans lequel R14 représente H, un groupe alkyle en C1 à C4, cycloalkyle en C3 à C6, benzyle, phényle, -C (O) R19 ou -S(O)2R19 ;

    où R19, indépendamment de n'importe quel autre groupe R19, représente un groupe alkyle en C1 à C6, cycloalkyle en C3 à C6, thiényle, furyle (furanyle) ou phényle ou benzyle ; dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués avec un ou deux substituants (indépendamment) fluoro, méthyle ou méthoxy ;

    et où ledit noyau hétérocyclique Het1 est facultativement substitué (à une ou plusieurs positions autres que n'importe quelle position de NR14) avec un ou deux substituants oxo (=O) et/ou un substituant alkyle en C1 à C4 ;

    sous réserve que, lorsque le noyau hétérocyclique Het1 contient un atome de O ou S dans le noyau et un groupe NR14 dans le noyau, alors : (a) l'atome de O/S du noyau et le groupe NR14 du noyau ne sont pas liés directement l'un à l'autre, et (b) l'atome de O/S du noyau et le groupe NR14 du noyau sont séparés par plus d'un atome de carbone à moins que Het1 contienne un groupement -NR14-C(O)-O- ou -NR19-C(O)-S- en tant qu'une partie du noyau ; ou

    -(CH2)n10-Ar, -CH-(alkyle en C1 ou C2)-Ar, -CMe2-Ar, ou cycloalkyle en C3 à C5 substitué au niveau de l'atome de carbone de connexion avec un substituant Ar, dans lequel n10 est égal à 0, 1 ou 2 et

    (i) Ar représente un groupe phényle facultativement substitué avec un ou deux substituants consistant indépendamment en des substituants fluoro, chloro, bromo, alkyle en C1 ou C2, fluoralkyle en C1 ou C2, alkoxy en C1 ou C2, fluoralkoxy en C1 ou C2, OH, -NR11aR11b (dans lequel R11b représente H ou un groupe alkyle en C1 ou C2 et R11b représente H, un groupe alkyle en C1 ou C2, -C(O)-(alkyle en C1 ou C2) ou -S (O)2-(alkyle en C1 ou c2)), cyano, -C(O)-NR11cR11d (dans lequel R11c et R11d représentent indépendamment H ou un groupe alkyle en C1 ou C2), -C(O)-OR11e dans lequel R11e représente H ou un groupe alkyle en C1 ou C2, ou -S(O)2-R11f dans lequel R11f représente un groupe alkyle en C1 ou C2, NH2, NHMe ou NMe2) ; ou bien le groupe phényle Ar est facultativement substitué au niveau de deux atomes adjacents du noyau Ar par les deux extrémités d'une chaîne qui est un groupe : -(CH2)4-, -(CH2)3- ou -CH=CH-CH=CH- ;
    ou

    (ii) Ar représente un noyau aromatique hétérocyclique penta- ou hexagonal facultativement substitué contenant 1, 2, 3 ou 4 hétéroatomes choisis entre O, N et S ; et dans lequel, lorsque le noyau aromatique hétérocyclique Ar contient 2, 3 ou 4 hétéroatomes, un hétéroatome est choisi entre O, N et S et le ou les hétéroatomes restants consistant en des atomes de N ; et dans lequel le noyau aromatique hétérocyclique Ar est facultativement substitué avec un ou deux groupes consistant indépendamment en des groupes alkyle en C1 à C4 ou OH (y compris n'importe quelle forme tautomère céto d'un noyau aromatique à substituant OH), ou bien le noyau aromatique hétérocyclique Ar est facultativement substitué au niveau de deux atomes adjacents du noyau Ar par les deux extrémités d'une chaîne qui est un groupe : -(CH2)4-, -(CH2)3- ou -CH=CH-CH=CH- ;

    RX1 et RY1 représentent indépendamment un atome d'hydrogène (H), un groupe alkyle en C1 ou C2 ou fluoralkyle en C1 ;

    RX3 et RX9 conjointement, représentent un groupe (CH2)n15-X7-(CH2)n16- dans lequel n15 et n16 sont égaux indépendamment à 1 ou 2 et X7 représente une liaison, un groupe -CH2-, 0 ou NRX5 dans lequel RX5 représente H, un groupe alkyle en C1 ou C2, acétyle ou -S(O)2Me ; et

    RZ représente un atome d'hydrogène (H) ;

    sous réserve que :

    lorsque R3 représente le groupe hétérocyclique de sous-formule (bb), n1 est égal à 1 et Y représente un groupe NR4, alors R4 ne représente pas un groupe alkyle en C1 ou C2 ou fluoralkyle en C1 ou C2 ;

    lorsque R3 représente le groupe hétérocyclique de sous-formule (aa) et Y représente un groupe NR4, alors R4 ne représente pas un groupe C(O)NH2, C(O)-(alkyle en C1 ou C2) ou C(O)-fluoralkyle en C1 ;

    sous réserve que :

    lorsque R3 représente le groupe hétérocyclique de sous-formule (cc), alors NR3R3a répond à la sous-formule (L), (m), (m1) ou (m2), dans laquelle le point de connexion -NH- du groupe NR3R3a à la position 4 de la pyrazolopyridine de formule (I) est souligné :

    et sous réserve que :

    lorsque R3 représente un groupe cycloalkyle en C3 en C8 facultativement substitué, alors n'importe quel substituant OH, alkoxy, fluoralkoxy, -CH2OH, -CH2NHR22, -C(O)OR23, -C(O)-NHR24, -C(O)R25 ou fluoro est en position 3, 4 ou 5 du noyau cycloalkyle R3 (dans lequel, dans cette connexion, la position 1 du noyau cycloalkyle R3 est considérée comme étant le point de connexion au groupe -NH- dans la formule (I)).


     
    2. Composé ou sel suivant la revendication 1, qui est un composé de formule (IA) ou un de ses sels :

    formule dans laquelle :

    R1 représente un groupe alkyle en C1 à C3, fluoralkyle en C1 ou C2 ou -(CH2)2OH ;

    R2 représente un atome d'hydrogène (H), un groupe méthyle ou fluoralkyle en C1 ;

    R3 représente un groupe cycloalkyle en C3 à C8 facultativement substitué, ou un groupe hétérocyclique, facultativement substitué de sous-formule (aa), (bb) or (cc) :

    dans laquelle n1 et n2 sont égaux indépendamment à 1 ou 2 ; et Y représente 0, S, un groupe SO2 ou NR4 ; dans lequel R4 représente un atome d'hydrogène (H), un groupe alkyle en C1 ou C2, fluoralkyle en C1 ou C2, C(O)NH2, C(O)-(alkyle en C1 ou C2) ou C(O)-(fluoralkyle en C1) ;
    dans lequel, dans R3, le groupe cycloalkyle en C3 à C8 est facultativement substitué avec un ou deux substituants consistant en des substituants oxo (=O), OH, alkoxy en C1, fluoralkoxy en C1 ou alkyle en C1 ou C2 ; et où n'importe quel substituant OH, alkoxy ou fluoralkoxy n'est pas substitué au niveau de l'atome de carbone de noyau R3 fixé (lié) au groupe -NH-de formule (IA) ;
    et où dans R3 le groupe hétérocyclique de sous-formule (aa), (bb) ou (cc) est facultativement substitué avec un ou deux substituants consistant en des substituants oxo (=O) ;
    et où Het répond à la sous-formule (i), (ii), (iii), (iv) ou (v) :

    dans laquelle :

    W1, W2, W4 et W5 représentent N ; et W3 représente un groupe NRW ;

    X1, X3 et X4 représentent N ou un groupe CRX ; X2 représente O, S ou un groupe NRX ; et X5 représente un groupe CRX1RX2 ;

    Y1, Y2 et Y3 représentent des groupes CRY ou N ; Y4 représente 0, S ou un groupe NRY ; et Y5 représente un groupe CRY1RY2 ;

    Z1 et Z5 représentent 0, S ou un groupe NRZ ; et Z2, Z3 et Z4 représentent un groupe N ou CRZ ;

    dans lesquels :

    RW représente un atome d'hydrogène (H) ;

    RX, RX2 , RY et RY2 représentent indépendamment :

    un atome d'hydrogène (H) ;

    un groupe alkyle en C1 à C8 ;

    un groupe cycloalkyle en C3 à C6 facultativement substitué avec un groupe alkyle en C1 ou C2 ;

    un groupe -(CH2)n2a- (cycloalkyle en C3 à C6) facultativement substitué, dans le groupement -(CH2)n2a- ou dans le groupement cycloalkyle en C3 à C6, avec un groupe alkyle en C1 ou C2, dans lequel n2a est égal à 1, 2 ou 3 ;

    un groupe -(CH2)n3-SO2-R5 dans lequel n3 est égal à 1 ou 2 et R5 représente un groupe alkyle en C1 à C3 ou -NH-(alkyle en C1 ou C2) ou phényle ;

    un groupe -(CH2)n4-NR6R7 dans lequel n4 est égal à 0, 1, 2 ou 3, et R6 et R7 représentent indépendamment H, un groupe alkyle en C1 à C6, cycloalkyle en C3 à C6, -CH2-(cycloalkyle en C3 à C6), -C(O)-(alkyle en C1 ou C2), -SO2-(alkyle en C1 ou C2), phényle ou benzyle (dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un substituant fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1 ou C2, alkoxy en C1 ou C2 ou fluoroalkoxy en C1) ; ou R6 et R7 conjointement représentent -(CH2)n5-X5-(CH2)n6- dans lequel n5 et n6 sont égaux indépendamment à 2 ou 3 et X5 représente une liaison, un groupe -CH2-, O ou NR8 dans lequel R8 représente H ou un groupe alkyle en C1 ou C2 ;

    un groupe -(CH2)n7-O-R9, dans lequel n7 est égal à 0, 1, 2 ou 3 et R9 représente H ou un groupe alkyle en C1 à C6 ; dans lequel n7 est égal à 0 seulement lorsque le groupe -(CH2)n7-O-R9 est lié à un atome de carbone dans le noyau Het ;

    et où n7 n'est pas égal à 0 lorsque Het répond à la sous-formule (v) (c'est-à-dire n7 n'est pas égal à 0 pour RX2 et pour RY2) ;

    un groupe -C (O) -NR10R11 dans lequel R10 et R11 représentent indépendamment H, un groupe alkyle en C1 à C6, cycloalkyle en C3 à C6, -CH2-(cycloalkyle en C3 à C6), phényle ou benzyle (dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un substituant fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2 ou fluoralkoxy en C1) ;

    ou bien R10 et R11, conjointement représentent un groupe -(CH2)n8-X6-(CH2)n9- dans lequel n8 et n9 sont égaux indépendamment à 2 ou 3 et X6 représente une liaison, un groupe -CH2-, O ou NR12 dans lequel R12 représente H ou un groupe alkyle en C1 ou C2 ;

    un groupe -C(O)-OR13 dans lequel R13 représente H, un groupe alkyle en C1 à C6, cycloalkyle en C3 à C6, -CH2-cycloalkyle en C3 à C6, phényle ou benzyle (dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un substituant fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2 ou fluoralkoxy en C1) ;

    un groupe -C(O)-R13a dans lequel R13a représente un atome d'hydrogène (H), un groupe alkyle en C1 à C6, fluoralkyle en C1 ou C2, cycloalkyle en C3 à C6, -CH2-(cycloalkyle en C3 à C6), benzyle ou phényle ; dans lequel les groupes phényle et benzyle sont indépendamment facultativement substitués sur le noyau aromatique avec un substituant fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1, alkoxy en C1 ou C2 ou fluoralkoxy en C1 ;

    un noyau hétérocyclique saturé tétra-, penta-, hexa- ou heptagonal contenant un atome de 0 dans le noyau ou un groupe NR14 dans le noyau, dans lequel R14 représente H ou un groupe alkyle en C1 à C4, ledit noyau hétérocyclique étant facultativement substitué (à une ou plusieurs positions autres que n'importe quelle position NR14) avec un substituant oxo (=O) et/ou un substituant alkyle en C1 à C4 ; ou

    un groupe -(CH2)n10-Ar dans lequel n10 est égal à 0, 1 ou 2 et

    (i) Ar représente un groupe phényle facultativement substitué avec un ou deux substituants consistant en des substituants fluoro, chloro, alkyle en C1 ou C2, fluoralkyle en C1 ou C2, alkoxy en C1 ou C2, fluoroalkoxy en C1 ou C2 ou cyano ;
    ou

    (ii)Ar représente un noyau aromatique hétérocyclique penta- ou hexagonal, facultativement substitué, contenant 1, 2 ou 3 hétéroatomes choisis entre 0, N et S ; ledit noyau aromatique hétérocyclique Ar contenant 2 ou 3 hétéroatomes, l'un étant choisi entre 0, N et S et le ou les hétéroatomes restants étant des atomes de N ; et où le noyau aromatique hétérocyclique Ar est facultativement substitué avec un ou deux groupes alkyle en C1 à C4 ;

    RX1 et RY1 représentent indépendamment un atome d'hydrogène (H), un groupe alkyle en C1 ou C2 ou un groupe fluoralkyle en C1 ; et

    RZ représente un atome d'hydrogène (H) ;
    sous réserve que :

    lorsque R3 représente le groupe hétérocyclique de sous-formule (bb), n1 est égal à 1, et Y représente un groupe NR4, alors R4 ne représente pas un groupe alkyle en C1 ou C2 ou un groupe fluoralkyle en C1 ou C2 ;

    lorsque R3 représente un groupe hétérocyclique de sous-formule (aa) et Y représente un groupe NR4, alors R4 ne représente pas un groupe C(O)NH2, C(O)-(alkyle en C1 ou C2) ou C(O)-(fluoralkyle en C1) ;

    sous réserve que :

    lorsque R3 représente le groupe hétérocyclique de sous-formule (cc), alors NHR3 répond à la sous-formule (L), (m) ou (m1), dans laquelle le point de connexion -NH- du groupe NHR3 à la position 4 de la pyrazolopyridine de la formule (IA) est souligné :

    et sous réserve que :

    lorsque R3 représente un groupe cycloalkyle en C3 à C8 facultativement substitué, alors n'importe quel substituant OH, alkoxy ou fluoroalkoxy est en position 3, 4 ou 5 du noyau cycloalkyle R3 (dans lequel, à cet égard, la position 1 du noyau cycloalkyle R3 est considéré comme étant le point de connexion au groupe -NH- dans la formule (IA)).


     
    3. Composé ou sel suivant la revendication 1 ou 2,
    dans lequel R2 représente un atome d'hydrogène (H) ou un groupe méthyle.
     
    4. Composé ou sel suivant la revendication 1, 2 ou 3, dans lequel R1 représente un groupe alkyle en C2 ou C3, fluoralkyle en C2 ou -(CH2)2OH.
     
    5. Composé ou sel suivant l'une quelconque des revendications précédentes, dans lequel R1 représente un groupe éthyle, n-propyle, fluoralkyle en C2 ou -CH2CH2OH.
     
    6. Composé ou sel suivant l'une quelconque des revendications précédentes dans lequel R1 représente un groupe éthyle.
     
    7. Composé ou sel suivant l'une quelconque des revendications précédentes dans lequel, dans R3, il existe un substituant ou bien il n'existe aucun substituant.
     
    8. Composé ou sel suivant l'une quelconque des revendications précédentes, dans lequel R3 représente un groupe cycloalkyle en C3 à C8 facultativement substitué ou le groupe hétérocyclique facultativement substitué, de sous-formule (aa), (bb) ou (cc).
     
    9. Composé ou sel suivant l'une quelconque des revendications précédentes, dans lequel, lorsque R3 représente le groupe hétérocyclique de sous-formule (aa), (bb) ou (cc), alors Y représente O.
     
    10. Composé ou sel suivant l'une quelconque des revendications précédentes, dans lequel, lorsque R3 représente le groupe hétérocyclique de sous-formule (aa), (bb) ou (cc), alors R3 représente le groupe hétérocyclique de sous-formule (bb) et n1 est égal à 1.
     
    11. Composé ou sel suivant l'une quelconque des revendications précédentes, dans lequel, dans R3, le groupe hétérocyclique de sous-formule (aa), (bb) ou (cc) est non substitué (où lorsque Y représente un groupe NR4, R4 n'est pas classé comme substituant).
     
    12. Composé ou sel suivant l'une quelconque des revendications précédentes, dans lequel le groupe NHR3 ou NR3R3a répond à la sous-formule (a), (a1), (b), (c), (c 1), (c 3), (c 4), (c 5), (d), (e), (f), (g), (g1), (g2), (g3), (g4), (h), (i), (j), (k), (k1), (L), (m), (m1), (m2), (m3), (m5), (n), (o), (o1), (o2), (o3), (o4), (p), (p2), (p3), (p5), (p7) ou (q), où le point de connexion -NH- du groupe NHR3 ou NR3NR3a à la position 4 de la pyrazolopyridine de la formule (I) ou (IA) est souligné :














     
    13. Composé ou sel suivant la revendication 12, dans lequel le groupe NHR3 ou NR3R3a répond à la sous-formule (h) ;


     
    14. Composé ou sel suivant la revendication 13, dans lequel R1 représente un groupe éthyle.
     
    15. Composé ou sel suivant l'une quelconque des revendications précédentes, dans lequel Het répond à la sous-formule (i), (ii) ou (v).
     
    16. Composé ou sel suivant la revendication 15, dans lequel Het répond à la sous-formule (ia), (ib), (ic), (id), (ie), (if), (ig), (va), (vb) ou (iia) :






     
    17. Composé ou sel suivant la revendication 16, dans lequel Het répond à la sous-formule (ia), (ic) ou (id).
     
    18. Composé qui est :

    la N-cyclopentyl-1-éthyl-5-(5-méthyl-1,3,4-oxadiazole-2-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la N-cyclopentyl-1-éthyl-5-{5-[(méthylsulfonyl)-méthyl]-1,3,4-oxadiazole-2-yl}-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la N-cyclopentyl-1-éthyl-5-(5-isopropyl-1,3,4-oxadiazole-2-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la N-cyclopentyl-1-éthyl-5-(5-méthyl-1,3,4-thiadiazole-2-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la N-cyclopentyl-1-éthyl-5-{5-[(méthylsulfonyl)-méthyl]-1,3,4-thiadiazole-2-yl}-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la N-cyclopentyl-1-éthyl-5-(5-isopropyl-1,3,4-thiadiazole-2-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-(5-isopropyl-1,3,4-oxadiazole-2-yl)-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la N-cyclohexyl-1-éthyl-5-(5-isopropyl-1,3,4-oxadiazole-2-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la N-cyclohexyl-1-éthyl-5-(5-méthyl-1,3,4-oxadiazole-2-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-(5-méthyl-1,3,4-oxadiazole-2-yl)-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-(5-tertiobutyl-1,3,4-oxadiazole-2-yl)-1-éthyl-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-(5-tertiobutyl-1,3,4-oxadiazole-2-yl)-N-cyclohexyl-1-éthyl-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-(5-tertiobutyl-1,3,4-oxadiazole-2-yl)-N-cyclopentyl-1-éthyl-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{5-[(méthylsulfonyl)méthyl]-1,3,4-oxadiazole-2-yl}-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la N-cyclohexyl-1-éthyl-5-{5-[(méthylsulfonyl)-méthyl]-1,3,4-oxadiazole-2-yl}-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-(3-méthyl-1,2,4-oxadiazole-5-yl)-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[3-(méthoxyméthyl)-1,2,4-oxadiazole-5-yl]-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 5-{3-[(diméthylamino)méthyl]-1,2,4-oxadiazole-5-yl}-1-éthyl-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-[3-(morpholine-4-ylméthyl)-1,2,4-oxadiazole-5-yl]-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo-[3,4-b]pyridine-4-amine,

    la 5-(5-cyclopropyl-1,3,4-oxadiazole-2-yl)-1-éthyl-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[ b]pyridine-4-amine,

    la N-(1-acétylpipéridine-4-yl)-1-éthyl-5-(5-méthyl-1,3,4-oxadiazole-2-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[5-(3-méthyloxétanne-3-yl)-1,3,4-oxadiazole-2-yl]-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{5-[(4-méthylpipérazine-1-yl)méthyl]-1,3,4-oxadiazole-2-yl}-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]pyridine-4-amine,

    le 5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-isopropyl-1,3,4-oxadiazole-2-carboxamide,

    la 4-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3,4-oxadiazole-2-yl]-1-méthylpyrrolidine-2-one,

    la 1-éthyl-N-tétrahydro-2H-pyranne-4-yl-5-(5-tétrahydro-2H-pyranne-4-yl-1,3,4-oxadiazole-2-yl)-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[5-(morpholine-4-ylméthyl)-1,3,4-oxadiazole-2-yl]-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-[5-(tertiobutoxyméthyl)-1,3,4-oxadiazole-2-yl]-1-éthyl-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]-pyridine-4-amine, ou

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3-oxazole-4-carboxylate de méthyle ;

    ou un de ses sels.
     
    19. Composé qui est :

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-4,5-dihydro-1,3-oxazole-4-carboxylate de méthyle,

    la 1-éthyl-5-(4-méthyl-4,5-dihydro-1,3-oxazole-2-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-(n-propyl)-5-(5-méthyl-1,3,4-oxadiazole-2-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-[5-(tétrahydrofuranne-2-yl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo-[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[5-(diméthylamino)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-(5-méthyl-1,2,4-triazole-3-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la N-(1-acétylpipéridine-4-yl)-1-éthyl-5-(3-méthyl-1,2,4-oxadiazole-5-yl)-1H-pyrazolo[3,4-b]pyridine-4-amzne, ou

    la N-(1-acétylpipéridine-4-yl)-1-éthyl-5-[3-(morpholine-4-ylméthyl)-1,2,4-oxadiazole-5-yl]-1H-pyrazolo-[3,4-b]-pyridine-4-amine ;
    ou un de ses sels.


     
    20. Composé qui est :

    la 1-éthyl-5-[(4R)-4-phényl-4,5-dihydro-1,3-oxazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[(4S)-4-phényl-4,5-dihydro-1,3-oxazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-[(4S)-4-(phénylméthyl)-4,5-dihydro-1,3-oxazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo-[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[(4R)-4-(phénylméthyl)-4,5-dihydro-1,3-oxazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo-[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[(4S,5R)-5-méthyl-4-phényl-4,5-dihydro-1,3-oxazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[(5R)-5-phényl-4,5-dihydro-1,3-oxazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-[(5S)-5-phényl-4,5-dihydro-1,3-oxazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 5-(4,4-diméthyl-4,5-dihydro-1,3-oxazole-2-yl)-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    l'acide 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3-oxazole-4-carboxylique,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-(1-méthyléthyl)-1,3-oxazole-4-carboxamide,

    1-éthyl-5-[4-(4-morpholinylcarbonyl)-1,3-oxazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    le trans-4-{[1-éthyl-5-(5-méthyl-1,3,4-oxadiazole-2-yl)-1H-pyrazolo [3,4-b]pyridine-4-yl]amino}cyclohexanol,

    la 1-éthyl-5-(5-méthyl-1,3,4-oxadiazole-2-yl)-N-(tétrahydro-2H-pyranne-3-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 4-{[1-éthyl-5-(5-méthyl-1,3,4-oxadiazole-2-yl)-1H-pyrazolo[3,4-b]pyridine-4-yl]amino}cyclohexanone,

    la 5-[5-(1,1-diméthyléthyl)-1,3,4-oxadiazole-2-yl]-1-éthyl-6-méthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo-[3,4-b]pyridine-4-amine,

    la 1-éthyl-6-méthyl-N-(tétrahydro-2H-pyranne-4-yl)-5-[5-(tétrahydro-2H-pyranne-4-yl)-1,3,4-oxadiazole-2-yl]-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-(5-cyclobutyl-1,3,4-oxadiazole-2-yl)-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo [3,4-b]pyridine-5-yl]-1,3,4-oxadiazole-2-yl}-2-pyrrolidinone,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3,4-oxadiazole-2-yl}-méthyl)acétamide,

    la 1-éthyl-5-[5-(1-méthyl-2-pipéridinyl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo [3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{5-[(4-méthyl-1,2,5-oxadiazole-3-yl)-méthyl]-1,3,4-oxadiazole-2-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 3-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3,4-oxadiazole-2-yl}-cyclopentanone,

    la 1-éthyl-5-[5-(tétrahydro-3-furannyl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la (4S)-4-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3,4-oxadiazole-2-yl}-1,3-thiazolidine-2-one,

    la 5-[5-(2,2-diméthylcyclopropyl)-1,3,4-oxadiazole-2-yl]-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo-[3,4-b]pyridine-4-amine,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3,4-oxadiazole-2-yl}-méthyl)-N-méthylacétamide,

    la 1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-5-[5-(tétrahydro-2H-pyranne-4-ylméthyl)-1,3,4-oxadiazole-2-yl]-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[5-(1-méthylcyclobutyl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-[5-(3-méthyl-5-isoxazolyl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo [3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[5-(1-méthyl-1H-pyrazole-5-yl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-[5-(1-acétyl-4-pipéridinyl)-1,3,4-oxadiazole-2-yl]-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo-[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{3-[(4-méthyl-1-pipérazinyl)méthyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[3-(4-fluorophényl)-1,2,4-oxadiazole-5-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine, ou

    la 1-éthyl-5-{3-[2-oxo-2-(1-pyrrolidinyl)éthyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo [3,4-b]pyridine-4-amine ;

    ou un de ses sels.
     
    21. Composé qui est :

    le 2-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-N-phényl-acétamide,

    le 2-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-N-(1-phényléthyl)-acétamide,

    la 1-éthyl-5-{3-[2-oxo-2-(1-pipéridinyl)éthyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo [3,4-b]pyridine-4-amine,

    le 2-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl]-N-(phénylméthyl)-acétamide,

    le 2-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-N,N-diméthyl-acétamide,

    le N-éthyl-2-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl)-acétamide,

    la 1-éthyl-5-{3-[1-(4-morpholinyl)éthyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-[3-(cyclohexylméthyl)-1,2,4-oxadiazole-5-yl]-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-{3-[2-oxo-2-(1-pipéridinyl)éthyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{3-[2-(4-méthyl-1-pipérazinyl)-2-oxo-éthyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-5-[5-(1H-1,2,3-triazole-1-ylméthyl)-1,3,4-oxadiazole-2-yl]-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-{5-[(2,4-diméthyl-1,3-thiazole-5-yl)méthyl]-1,3,4-oxadiazole-2-yl}-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[5-(2-furannylméthyl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-[5-(3-isoxazolylméthyl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo-[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-(5-{[4-(méthyloxy)phényl]méthyl}-1,3,4-oxadiazole-2-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-5-[5-(1H-tétrazole-1-ylméthyl)-1,3,4-oxadiazole-2-yl]-1H-pyrazolo-[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[5-(5-isothiazolylméthyl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{5-[(3-méthyl-5-isoxazolyl)méthyl]-1,3,4-oxadiazole-2-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-(5-{[4-(diméthylamino)phényl]méthyl}-1,3,4-oxadiazole-2-yl)-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine (1 : 1),

    la 1-éthyl-5-{5-[(2-méthyl-1,3-thiazole-4-yl)méthyl]-1,3,4-oxadiazole-2-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    le 2-[1-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3,4-oxadiazole-2-yl}méthyl)cyclopentyl]-N-méthylacétamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3,4-oxadiazole-2-yl}méthyl)cyclopropane-carboxamide,

    la 1-éthyl-5-{5-[(5-méthyl-3-isoxazolyl)méthyl]-1,3,4-oxadiazole-2-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{5-[(5-méthyl-3-isoxazolyl)méthyl]-1,3,4-oxadiazole-2-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{5-[2-(4-méthyl-1,3-thiazole-5-yl)-éthyl]-1,3,4-oxadiazole-2-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-{5-[(3,5-diméthyl-4-isoxazolyl)méthyl]-1,3,4-oxadiazole-2-yl}-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    le N-(1-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3,4-oxadiazole-2-yl}éthyl)-acétamide,

    la 5-{5-[(1-acétyl-4-pipéridinyl)méthyl]-1,3,4-oxadiazole-2-yl}-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{5-[(4-méthylphényl)méthyl]-1,3,4-oxadiazole-2-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[5-(4-méthylphényl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 5-[5-(3,4-diméthylphényl)-1,3,4-oxadiazole-2-yl]-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 5-[5-(2,4-diméthylphényl)-1,3,4-oxadiazole-2-yl]-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 5-{5-[(4-bromophényl)méthyl]-1,3,4-oxadiazole-2-yl}-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo-[3,4-b]pyridine-4-amine,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-(phénylméthyl)-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-{[4-(méthyloxy)phényl]-méthyl}-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-[(2-méthylphényl)méthyl]-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-[(4-méthylphényl)méthyl]-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-[(3-méthylphényl)méthyl]-1,3-oxazole-4-carboxamide,

    le N-[(4-chlorophényl)méthyl]-2-[1-éthyl-4-(tétra-hydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]-pyridine-5-yl]-1,3-oxazole-4-carboxamide,

    le N-[(2,3-diméthylphényl)méthyl]-2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]-pyridine-5-yl]-1,3-oxazole-4-carboxamide,

    le N-[(3,5-diméthylphényl)méthyl]-2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]-pyridine-5-yl]-1,3-oxazole-4-carboxamide,

    le N-[(3,4-diméthylphényl)méthyl]-2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]-pyridine-5-yl]-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-(1-phényléthyl)-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-{(1R)-[4-(méthyloxy)-phényl]éthyl }-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-[(1R)-1-phénylpropyl]-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-(4-méthylphényl)-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-({4-[(méthylsulfonyl)-amino]phényl}méthyl)-1,3-oxazole-4-carboxamide,

    le [1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-{[4-(méthylsulfonyl)-phényl]méthyl}-1,3-oxazole-4-carboxamide,

    le N-(1-acétyl-4-pipéridinyl)-2-[1-éthyl-4-(tétra-hydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]-pyridine-5-yl]-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-(tétrahydro-2-furanyl-méthyl)-1,3-oxazole-4-carboxamide,

    le 2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-N-[2-(4-méthyl-1-pipérazinyl)-éthyl]-1,3-oxazole-4-carboxamide,

    le N-[1-(aminométhyl)cyclohexyl]-2-[1-éthyl-4-(tétra-hydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b] pyridine-5-yl]-N-méthyl-1,3-oxazole-4-carboxamide,

    le N-(2,6-diméthylphényl)-2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3-oxazole-4-carboxamide,

    le N-{[4-(aminocarbonyl)phényl]méthyl}-2-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]-pyridine-5-yl]-1,3-oxazole-4-carboxamide,

    le 2-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl]-N-(tétrahydro-2H-pyranne-4-yl)-acétamide,

    le 5-{3-[2-(2,6-diméthyl-4-morpholinyl)-2-oxoéthyl]-1,2,4-oxadiazole-5-yl}-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    le 1-éthyl-5-{3-[2-(4-méthyl-1-pipéridinyl)-2-oxo-éthyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    le 2-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-N-[1-méthyl-2-(méthyloxy)éthyl]-acétamide,

    le 5-{3-[2-(3,5-diméthyl-1-pipéridinyl)-2-oxoéthyl]-1,2,4-oxadiazole-5-yl}-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{3-[2-(3-méthyl-1-pipéridinyl)-2-oxo-éthyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    le 2-{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-N-3-pyridinyl-acétamide,

    la 6{5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,3,4-oxadiazole-2-yl]-2-pipéridinone,

    la 1-éthyl-5-{5-[(3-méthyl-1H-1,2,4-triazole-5-yl)-méthyl]-1,3,4-oxadiazole-2-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}méthyl)-acétamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}méthyl)benzamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)-2-phénylacétamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)-2-méthyl-propanamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}méthyl)-3-méthyl-butanamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)cyclohexane-carboxamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)-2-furanne-carboxamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)méthane-sulfonamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)benzène-sulfonamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)-1-phénylméthane-sulfonamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}méthyl)-2-propane-sulfonamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)-1-propane-sulfonamide,

    le N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)cyclopropane-sulfonamide,

    N-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-ylamino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl) méthyl)-2-thiophène-sulfonamide,

    la 1-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)-2-pyrrolidinone,

    la 1-({5-[1-éthyl-4-(tétrahydro-2H-pyranne-4-yl-amino)-1H-pyrazolo[3,4-b]pyridine-5-yl]-1,2,4-oxadiazole-3-yl}-méthyl)-2-pipéridinone,

    la 5-{3-[(1-acétyl-4-pipéridinyl)méthyl]-1,2,4-oxadiazole-5-yl}-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-(3-{[1-(3-méthylbutanoyl)-4-pipéridinyl]-méthyl}-1,2,4-oxadiazole-5-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-(3-{[1-(méthylsulfonyl)-4-pipéridinyl]-méthyl}-1,2,4-oxadiazole-5-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{3-[1-(phénylsulfonyl)cyclopropyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-[3-(phénylméthyl)-1,2,4-oxadiazole-5-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-[3-(1-phényléthyl)-1,2,4-oxadiazole-5-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-5-(3-{[4-(méthyloxy)phényl]méthyl}-1,2,4-oxadiazole-5-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-(3-{[4-(diméthylamino)phényl]méthyl}-1,2,4-oxadiazole-5-yl)-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-(3-{[3-(diméthylamino)phényl]méthyl}-1,2,4-oxadiazole-5-yl)-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-(3-{[4-(diméthylamino)phényl]méthyl}-1,2,4-oxadiazole-5-yl)-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{3-[(phényloxy)méthyl]-1,2,4-oxadiazole-5-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine,

    la 1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-5-[3-(5,6,7,8-tétrahydro[1,2,4]triazolo[4,3-a]pyridine-3-yl-méthyl)-1,2,4-oxadiazole-5-yl]-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-{3-[(4-phényl-1-pipérazinyl)méthyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-(5-éthyl-1,2,4-oxadiazole-3-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 5-(5-{[4-(diméthylamino)phényl]méthyl}-1,2,4-oxadiazole-3-yl)-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine,

    la 1-éthyl-5-(5-{[4-(méthyloxy)phényl]méthyl}-1,2,4-oxadiazole-3-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo-[3,4-b]pyridine-4-amine, ou

    la 5-(3,8-dioxa-1-azaspiro[4.5]déc-1-ène-2-yl)-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine ;

    ou un de ses sels.
     
    22. Composé qui est :

    la 1-éthyl-5-(5-méthyl-1,3,4-oxadiazole-2-yl)-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine (le composé de l'exemple 14),

    la 5-(5-tertiobutyl-1,3,4-oxadiazole-2-yl)-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine (le composé de l'exemple 17),

    la 1-éthyl-5-{5-[(méthylsulfonyl)méthyl]-1,3,4-oxadiazole-2-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine (le composé de l'exemple 23),

    la 1-éthyl-5-[5-(3-méthyloxétanne-3-yl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine (le composé de l'exemple 34),

    la 1-éthyl-5-{5-[(4-méthylpipérazine-1-yl)méthyl]-1,3,4-oxadiazole-2-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine (le composé de l'exemple 35),

    la 1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-5-[5-(tétrahydro-2H-pyranne-4-yl)-1,3,4-oxadiazole-2-yl]-1H-pyrazolo[3,4-b]pyridine-4-amine (le composé de l'exemple 38), appelé également :

    la 1-éthyl-5-[5-(morpholine-4-ylméthyl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo-[3,4-b]pyridine-4-amine (le composé de l'exemple 39),

    la 1-éthyl-5-[5-(tétrahydrofuranne-2-yl)-1,3,4-oxadiazole-2-yl]-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine (le composé de l'exemple 44),

    la 1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-5-[5-(tétrahydro-2H-pyranne-4-ylméthyl)-1,3,4-oxadiazole-2-yl]-1H-pyrazolo[3,4-b]pyridine-4-amine (le composé de l'exemple 77), ou

    la 1-éthyl-5-{3-[2-oxo-2-(1-pyrrolidinyl)éthyl]-1,2,4-oxadiazole-5-yl}-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine (le composé de l'exemple 84) ;

    ou un de ses sels.
     
    23. Composé ou sel suivant la revendication 22, qui est la 1-éthyl-5-(5-méthyl-1,3,4-oxadiazole-2-yl)-N-(tétra-hydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]-pyridine-4-amine

    ou un de ses sels.
     
    24. Composé ou sel suivant la revendication 18, qui est la 5-(5-cyclopropyl-1,3,4-oxadiazole-2-yl)-1-éthyl-N-tétrahydro-2H-pyranne-4-yl-1H-pyrazolo[3,4-b]pyridine-4-amine

    ou un de ses sels.
     
    25. Composé ou sel suivant la revendication 20, qui est la 1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-5-[5-(tétra-hydro-2H-pyranne-4-ylméthyl)-1,3,4-oxadiazole-2-yl]-1H-pyrazolo-[3,4-b]pyridine-4-amine

    ou un de ses sels.
     
    26. Composé ou sel suivant la revendication 21, qui est la 5-{5-[(2,4-diméthyl-1,3-thiazole-5-yl)méthyl]-1,3,4-oxadiazole-2-yl}-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine

    dans laquelle Ry représente un groupe

    ou un de ses sels.
     
    27. Composé ou sel suivant la revendication 21, qui est la 5-{5-[(2,4-diméthyl-1,3-thiazole-5-yl)méthyl]-1,3,4-oxadiazole-2-yl}-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine

    dans laquelle Ry représente un groupe

    ou un de ses sels pharmaceutiquement acceptables.
     
    28. Composé ou sel suivant la revendication 21, qui est la 5-{5-[(2,4-diméthyl-1,3-thiazole-5-yl)méthyl]-1,3,4-oxadiazole-2-yl}-1-éthyl-N-(tétrahydro-2H-pyranne-4-yl)-1H-pyrazolo[3,4-b]pyridine-4-amine

    dans laquelle Ry représente un groupe


     
    29. Composé, ou un de ses sels pharmaceutiquement acceptables, suivant l'une quelconque des revendications précédentes, destiné à être utilisé comme substance thérapeutique active chez un mammifère tel qu'un être humain.
     
    30. Composition pharmaceutique comprenant un composé tel que défini dans l'une quelconque des revendications 1 à 28, ou un de ses sels pharmaceutiquement acceptables, et un
    ou plusieurs supports et/ou excipients pharmaceutiquement acceptables.
     
    31. Composition pharmaceutique suivant la revendication 30, qui est apte à, et/ou adaptée à, l'administration orale.
     
    32. Composition pharmaceutique suivant la revendication 30 ou 31, pour le traitement et/ou la prophylaxie d'une maladie inflammatoire et/ou allergique, ou d'une altération cognitive dans un trouble neurologique, chez un mammifère tel qu'un être humain.
     
    33. Utilisation d'un composé tel que défini dans l'une quelconque des revendications 1 à 28, ou d'un de ses sels pharmaceutiquement acceptables, dans la production d'un médicament destiné au traitement et/ou à la prophylaxie d'une maladie inflammatoire et/ou allergique,
    ou d'une altération cognitive dans un trouble neurologique, chez un mammifère tel qu'un être humain.
     
    34. Utilisation d'un composé tel que défini dans l'une quelconque des revendications 1 à 28, ou d'un de ses sels pharmaceutiquement acceptables, dans la production d'un médicament pour le traitement et/ou la prophylaxie de : l'asthme, une maladie pulmonaire obstructive chronique (COPD), la dermatite atopique, l'urticaire, la rhinite allergique, la conjonctivite allergique, la conjonctivite printanière, le granulome éosinophile, le psoriasis, la polyarthrite rhumatoïde, le choc septique, la colite ulcérative, la maladie de Crohn, une lésion de reperfusion du myocarde et du cerveau, la glomérulonéphrite chronique, un choc endotoxique, le syndrome de détresse respiratoire de l'adulte, la sclérose en plaques, une altération cognitive dans un trouble neurologique, la dépression ou la douleur ; chez un être humain.
     
    35. Composition pharmaceutique suivant la revendication 30, destinée à être utilisée dans le traitement et/ou la prophylaxie de : l'asthme, une maladie pulmonaire obstructive chronique (COPD), la dermatite atopique, l'urticaire, la rhinite allergique, la conjonctivite allergique, la conjonctivite printanière, un granulome éosinophile, le psoriasis, la polyarthrite rhumatoïde, un choc septique, la colite ulcérative, la maladie de Crohn, une lésion de reperfusion du myocarde et du cerveau, la glomérulonéphrite chronique, un choc endotoxique, le syndrome de détresse respiratoire de l'adulte, la sclérose en plaques, une altération cognitive dans un trouble neurologique, la dépression ou la douleur ; chez un être humain.
     
    36. Composé ou un de ses sels pharmaceutiquement acceptables, suivant l'une quelconque des revendications 1 à 28, destiné au traitement et/ou à la prophylaxie de la dépression chez un être humain.
     
    37. Composé ou un de ses sels pharmaceutiquement acceptables, suivant l'une quelconque des revendications 1 à 28, destiné au traitement et/ou à la prophylaxie d'une altération cognitive chez un être humain.
     
    38. Composé ou un de ses sels pharmaceutiquement acceptables, suivant la revendication 37, dans lequel l'altération cognitive est une altération cognitive dans un trouble neurologique.
     
    39. Composé ou un de ses sels pharmaceutiquement acceptables, suivant la revendication 38, dans lequel le trouble neurologique est la maladie d'Alzheimer.
     
    40. Utilisation d'un composé ou d'un de ses sels pharmaceutiquement acceptables, suivant l'une quelconque des revendications 1 à 28, dans la production d'un médicament destiné au traitement et/ou à la prophylaxie de la dépression chez un être humain.
     
    41. Utilisation d'un composé ou d'un de ses sels pharmaceutiquement acceptables, suivant l'une quelconque des revendications 1 à 28, dans la production d'un médicament destiné au traitement et/ou à la prophylaxie d'une altération cognitive chez un être humain.
     
    42. Utilisation d'un composé ou d'un de ses sels pharmaceutiquement acceptables, suivant la revendication 41, dans laquelle l'altération cognitive est une altération cognitive dans un trouble neurologique.
     
    43. Utilisation d'un composé ou d'un de ses sels pharmaceutiquement acceptables, suivant la revendication 42, dans lequel le trouble neurologique est la maladie d'Alzheimer.
     




    REFERENCES CITED IN THE DESCRIPTION



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    Patent documents cited in the description




    Non-patent literature cited in the description