(19)
(11)EP 0 568 126 A1

(12)EUROPEAN PATENT APPLICATION

(43)Date of publication:
03.11.1993 Bulletin 1993/44

(21)Application number: 93200950.9

(22)Date of filing:  01.04.1993
(51)International Patent Classification (IPC)5G01N 33/50, G01N 21/64
(84)Designated Contracting States:
AT BE CH DE DK ES FR GB GR IE IT LI LU MC NL PT SE

(30)Priority: 06.04.1992 IT MI920829

(71)Applicants:
  • Doglia, Silvia Maria
    I-20129 Milano (IT)
  • Villa, Anna Maria
    I-24068 Seriate, Bergamo (IT)
  • Manfait, Michel
    51100 Reims (FR)

(72)Inventors:
  • Doglia, Silvia Maria
    I-20129 Milano (IT)
  • Villa, Anna Maria
    I-24068 Seriate, Bergamo (IT)
  • Manfait, Michel
    51100 Reims (FR)

(74)Representative: Riccardi, Sergio 
Riccardi & Co. Via Macedonio Melloni, 32
20129 Milano
20129 Milano (IT)


(56)References cited: : 
  
      


    (54)Method for the diagnosis of multidrug resistance in living cells


    (57) A method is described to determine multidrug resistance in living cells by laser scanning confocal fluorescence microscopy of doxorubicin in sensitive and resistant tumor cells. The intracellular distribution of doxorubicin in K562 cells has been studied by laser scanning confocal fluorescence microscopy. A different fluorescence pattern has been observed in sensitive and resistant cells. An intense fluorescence signal is evident on the plasma membrane of K562R cells with multidrug resistance. The fluorescence imaging of the drug offers then a new method - non destructive - to discriminate between sensitive and resistant cells, with high potential in the diagnosis of resistance in tumors in vivo. The differences in the intracellular drug distribution, as seen by laser scanning confocal fluorescence microscopy, moreover offer new indications on the mode of action of the drug in the living cell.




    Description

    Field and background of the invention.



    [0001] A major obstacle in cancer chemotherapy is the emergence of resistance to cytotoxic drugs in tumor cells during treatment. Two central problems are still open: 1) the understanding at molecular level of the mechanism by which cells develop resistance; 2) the acquisition of a simple and rapid method for the diagnosis of resistance in cancer chemotherapy.

    [0002] The resistant phenotype is characterized by a loss of sensitivity not always against a single drug, but towards a number of chemically unrelated molecules, a behavior known as "Multidrug resistance" (MDR) (1,2).

    [0003] This complex mechanism is characterized by a reduced intracellular accumulation of the drug resulting from an increased efflux across the plasma membrane (3-5) or from an energy-dependent permeability barrier, which controls the drug entry.(6-9). These mechanisms involve a change in the cell phenotype, which appears at the plasma membrane level. Indeed an altered plasmalemmal composition characterizes MDR cells, with changes in the surface glycoproteins and overexpression of a P-glycoprotein of molecular weight = 170-180 kD (8,10).

    [0004] Several changes in the cell molecular architecture have been recognized (1); however plasma membrane alterations remain the distinctive characteristics of MDR.

    Description of the invention.



    [0005] In this report we suggest that differences in the intracellular drug distribution, as seen by confocal fluorescence microscopy, could offer a simple and crucial test to characterize resistant and sensitive tumor cells, and give new insights into the mode of action of the drug in living cells.

    [0006] Although a great deal has been learned about doxorubicin, an antitumoral antibiotic belonging to the antracycline class, the intracellular distribution of the drug - at pharmacological concentrations - is not yet known at the spatial resolution now allowed by confocal microscopy. We present here confocal fluorescence imaging data of doxorubicin in human leukemia K562 cells (12), a well known biological model of multidrug resistance for its strong refractoriness to doxorubicin and overexpression of the membrane P-glycoprotein (13).

    [0007] The laser scanning confocal fluorescence microscope MRC-600 (Lasersharp, Bio-Rad Microscience Division) was coupled to an epifluorescence microscope . An Argon laser was used for excitations at 488 nm and 515 nm, falling within the broad visible absorption band of doxorubicin (11,14). Fast photon counting mode has been employed to detect the drug fluorescence signal, through a long pass filter in the emission fluorescence band of the drug, above 515 nm (14). The high sensitivity of the photon counting detection allowed :1) to keep laser excitation at low power (<0.1 mW), preserving cell viability during measurements, and 2) to collect high quality images from low drug concentrations, in the range of pharmacological significance. A high level of confocality has been kept in the optics. In this way it has been possible to image the cell within a narrow depth of focus (0.5 µm) and to obtain optical-section images in the Z-direction, together with a very high spatial resolution in the image plane (15).

    [0008] K562 sensitive and resistant cells (12,13) in exponential growth phase (105 cells/ml) were incubated for 1 hour in culture medium containing doxorubicin (DXR) (Farmitalia- Carlo Erba, Milan, Italy; and Roger Bellon Laboratories, Paris, France) at a concentration varying from 2 to 0.01 µM . Cells were washed twice and resuspended in PBS.

    [0009] Oil immersion objective, with 60x magnification and N.A. 1.4, in the epi-illuminated microscope coupled to the MRC-600 system was employed for measurements on living cells.

    [0010] In Fig 1 and 2a are reported the fluorescence images of K562 S living cells, incubated for 1 hour at 0.1 µM and at 0.01 µM respectively .A non homogeneous drug fluorescence can be recognized within the nucleus .The highest nuclear fluorescence appears in the perinuclear region, as recently reported also for daunorubicin when cells are examined under living conditions(16).

    [0011] No membrane fluorescence is observed in sensitive cells, at any drug concentration and at any time after incubation. A brilliant fluorescence is instead observed in the K562 S cytoplasm .This sharp signal, evident only in living cells, can be attributed to the Golgi membranes system of the cell .This is easily seen by comparison with the data of Lipsky and Pagano (17) on C6-NBD-ceramide, a vital stain of the Golgi apparatus. Indeed, this signal is present only when the Golgi element belongs to the focal plane of the image ; it is enough to move the focus of about 4 µm in the Z-direction in order to eliminate completely its fluorescence from the image. This is shown for K562 R cells incubated for 1hour in a 0.01 µM doxorubicin solution in Fig 3a and 3b where two optical sections 4 µm apart are reported. Moreover it is interesting to remark that the spatial resolution of the confocal images (15) allows the examination of the Golgi morphology in the living cell by means of doxorubicin, that can be seen therefore as a vital fluorescent stain of this organelle. We add a final comment on the images of sensitive cells concerning the drug fluorescence in the cytoplasm, where ordered filamentary structures can be frequently seen (Fig. 1). Although it is impossible at this stage to recognize these structures, they are an indication of the plurality of drug interactions in the cell.

    [0012] Considering now the results obtained on resistant living cells, the doxorubicin pattern is very different from that observed on sensitive living cells.

    [0013] If we compare the data of Fig 2a and Fig.2b (see also Fig. 1) a major difference is apparent in the nuclear region, where almost no fluorescence is observed in resistant cells in agreement with the results reported for daunorubicin in reference 16. This is also confirmed by the Z-sectioning study. (Fig. 4)
    A second important difference between sensitive and resistant cells is the intense fluorescence of the plasma membrane of resistant cells ,as shown in Fig. 2a and 2b, where the images of K562S and R cells treated with doxorubicin at 0.01 µM for 1 hour are reported The membrane fluorescence is not homogeneously distributed along the membrane, with evident filaments emerging into the extracellular medium. A bright fluorescence of the Golgi system is also evident in resistant cells ,when this organelle belongs to the image plane .(Fig 3 a and b)
    A further important detail in the fluorescence of resistant cells is the pattern of weak fluorescent spots , regularly distributed in defined areas of the cytoplasm.(Fig. 2b, 2a, and 3b) This pattern of spot-like signals varies with drug concentration and with time after the incubation. This "punctuate pattern" , observed also in tumor cells resistant to daunorubicin, has been suggested (16) to be a characterizing feature of resistance
    In the case of Multidrug Resistance examined here ,with overexpression of membrane P-glycoprotein, the characterizing feature of resistance is the bright fluorescence of plasma membrane. In K562R cells treated with doxorubicin this membrane fluorescence can be detected by laser scanning confocal fluorescence microscopy , in the range of drug concentrations of pharmacological significance. The same membrane fluorescence in K562R can also be induced by other vital dyes such as acridine orange (19) and dihydroethidium (20) and fluorescent probes and cations such as ethidium bromide, propidium and rhodamine 123 (21).

    [0014] This result is in agreement with the localizations proposed for the P-glycoprotein (18), which is found in the plasma membrane, with highest concentration, and in the luminal side of the Golgi membranes, as expected for a transport protein. The doxorubicin fluorescent pattern of the multidrug resistant cells appears then to coincide with the P-glycoprotein localization.

    [0015] The differences on the drug fluorescence patterns in sensitive and resistant K562 cells suggest, therefore, a simple and rapid method to detect multidrug resistance in tumor cells (and particularly in leukemia ). The non-destructive character of this technique indicates possible applications in the screening of tumors in vivo, an important goal in cancer chemotherapy.

    References.



    [0016] 

    1) G.Bradley,P.F.Juranka and V.Ling, BBA 948, (1988)87-128

    2)J.A.Moscow,K.H.Cowan, J. Hat. Cancer Institute 80, (1988)14-20.

    3) T. Skousgaard, Cancer Research 38, (1978)1785-1791.

    4) M.Inaba, H.Kobaiyashi, Y.Sakurai, and R.K.Johnson ,Cancer Res.39 (1979)2200-2203.

    5) T.Tsuruo, H.Ida, S.Tsukagoshi, and Y. Sakurai, Cancer Research 41(1981) 1967-1972; idem CancerResearch 42(1982)4730-4733.

    6) V. Ling, Carlsen S.A. and Y.P.Yee in "Membrane toxicity" (Miller, M.W. and Shamoo Eds.) Plenum Press (1977) pp.247-264.

    7) J.L.Biedler,and R.H.F.Peterson in "Molecular Actions and Targets for Cancer Chemiotherapeutic Agents" (A.C. Sartorelli, J.S.Lazo, and J.R.Bertino Eds.) Academic Press (1981) pp. 453-482.

    8) N.Kartner, J.R.Riordan, G.Bradley, and V.Ling, Science 221(1983)1285-1288.

    9) N.Kartner, D. Evernden-Porelle, G.Bradley, and V.Ling, Nature 316(1985)820-823

    10) H.Hamada and T.Tsuruo, J.Biol. Chem.263 (1988)1454-1458

    11) A. Di Marco, Cancer chemother. Rep. 32(1975)48

    12) C. B. Lozzio and B. B. Lozzio, Blood 45(1975)321-334

    13) T. Tsuruo et al., Jpn. J. Cancer Res. 77(1986)682-692

    14) M. Gigli, S. M. Doglia, J. M. Millot, L. Valentini and M. Manfait, BBA 950(1988)13 ; M. Gigli et al., Cancer Res. 49(1989)560.

    15) Confocal Microscopy ,Ed: T:Wilson ,Academic Press (1990)

    16) J.E.Gervasoni,Jr. et al.,Cancer Research 51,4955(1991).

    17) H. G. Lipsky and R. Pagano, Science 228,745(1985)

    18) M. C. Willingham et al., J. Hist. and Cytochem.88, 1451(1987)

    19) G. Brandes and E. Reale, Histochem. Journal, 22,106-112(1990)

    20) C. Bucana, I. Saiki, and R. Nayar, J. Hist. and Cytochem. 34,1109-1115(1986)

    21) J. L. Weaver, P. S. Pine, A. Aszalos, P. V. Schoenlein, S. J. Currier, R. Padmanabhan, and M. M. Gottesman, Exp. Cell Research, 196,323-329(1991)


    FIGURE CAPTIONS.



    [0017] Fig. 1: Confocal fluorescence image of DXR in K562S living cell in suspension, incubated for 1 hour in DXR 0.1 µM collected by fast photon counting mode with Argon laser excitation at 488 nm and emission through Long Pass filter 515 nm, (accumulation of 900 frames).

    [0018] Fig.2: Confocal fluorescence image of DXR in living cells in suspension, incubated for 1 hour in DXR 0.01 µM, collecting conditions as in Fig.1: a) K562S accumulation of 323 frames; b)K562R accumulation of 320 frames.

    [0019] Fig.3: Confocal fluorescence image of DXR in living K562R cell in suspension, incubated for 1 hour in DXR 0.01 µM, collecting conditions as in Fig.1 (accumulation of 320 frames): a) the focal plane of the image is adjusted on the Golgi element of the cell; b) the focal plane is moved 4 µM above.

    [0020] Fig.4: Z-sectioning fluorescence images of K562R living cells incubated in DXR 0.1µM for 1 hour; series of images at planes with separation distance of 2 µm. Accumulation of 150 frames (collecting conditions as in Fig. 1).


    Claims

    1. A method to determine Multidrug Resistance in living cells ,characterized by the determination of the intracellular distribution of an antitumor drug in livng tumor cells through the analysis of the differences in the fluorescent patterns of sensitive and resistant cells obtained by laser scanning confocal fluorescence microscopy.
     
    2. A method as claimed in claim 1, wherein the antitumor drug employed as vital stain of cells in order to induce fluorescence is doxorubicin.
     
    3. A method as claimed in claim 1 ,wherein the tumor cells employed are human leukemia cells of type K562.
     
    4. A method as claimed in any one of claims 1 to 3, wherein the Multidrug Resistance is determined by the presence of an intense fluorescent signal on the living cells membrane.
     
    5. A method as claimed in any one of the claims 1 to 4 ,wherein the membrane fluorescence signal is detected by confocal fluorescence microscopy, which allows to collect images of the cell optical sections in the z-axis direction.
     
    6. A method as claimed in any one of claims from 1 to 5 ,wherein the membrane fluorescence of multidrug resistant cells can be detected also by fluorescent vital dyes such as acridine orange , and dihydroethidium, and by fluorescent probes such as ethidium bromide , propidium bromide and rhodamin 123.
     




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