(19)
(11)EP 2 264 439 A3

(12)EUROPEAN PATENT APPLICATION

(88)Date of publication A3:
19.01.2011 Bulletin 2011/03

(43)Date of publication A2:
22.12.2010 Bulletin 2010/51

(21)Application number: 10012856.0

(22)Date of filing:  09.11.2000
(51)Int. Cl.: 
G01N 21/64  (2006.01)
B01L 7/00  (2006.01)
G01N 21/25  (2006.01)
(84)Designated Contracting States:
CH DE FR GB IT LI NL

(30)Priority: 12.11.1999 US 165267 P

(62)Application number of the earlier application in accordance with Art. 76 EPC:
08003717.9 / 1921440
00979148.4 / 1228357

(71)Applicant: E.I. DU PONT DE NEMOURS AND COMPANY
Wilmington, DE 19805 (US)

(72)Inventors:
  • Dabell, Stanley Davida
    Newark, Delaware 19702 (US)
  • Lee, jerald D.
    Mendenhall, Pennsylvania 19357 (US)

(74)Representative: Hughes, Andrea Michelle 
Dehns St Bride's House 10 Salisbury Square
London EC4Y 8JD
London EC4Y 8JD (GB)

  


(54)Fluorometer with low heat-generating light source


(57) A combined fluorometer and thermal cycler, comprising:
a plurality of low heat-generating light sources (20);
a plurality of containers (32) having caps; means for positioning said plurality of containers into optical communication with said plurality of light sources (20), wherein each of said light sources (20) communicates with one of said containers;
a thermally controlled base in close contact with said plurality of containers and a thermally controlled cover having a plurality of apertures, wherein each of said apertures corresponds with one of said containers (32) and expands outward from said cap of said corresponding container (32);
a first optical path means for guiding light from said plurality of light sources to said plurality of containers;
an optical signal sensing means (36) in optical communication with said plurality of containers (32);
a second optical path means for guiding emitted light from any sample in said plurality of containers (32) to said optical signal sensing means (36); and
programmable control means (500) for controlling the temperature of said plurality of containers (32) according to a selected protocol.