(19)
(11)EP 2 267 033 A3

(12)EUROPEAN PATENT APPLICATION

(88)Date of publication A3:
30.03.2011 Bulletin 2011/13

(43)Date of publication A2:
29.12.2010 Bulletin 2010/52

(21)Application number: 10010599.8

(22)Date of filing:  31.03.2005
(51)Int. Cl.: 
C07K 16/44  (2006.01)
C12N 15/63  (2006.01)
G01N 33/532  (2006.01)
C12N 15/09  (2006.01)
G01N 33/53  (2006.01)
(84)Designated Contracting States:
BE CH DE FR GB LI SE

(30)Priority: 31.03.2004 JP 2004108388

(62)Application number of the earlier application in accordance with Art. 76 EPC:
05728901.9 / 1732952

(71)Applicant: Canon Kabushiki Kaisha
Tokyo (JP)

(72)Inventors:
  • Shiotsuka, Hidenori
    Tokyo (JP)
  • Imamura, Takeshi
    Tokyo (JP)
  • Kumagai, Izumi
    Tokyo (JP)

(74)Representative: Weser, Thilo et al
Weser & Kollegen Patentanwälte Radeckestraße 43
D-81245 München
D-81245 München (DE)

  


(54)Gold-binding protein and use thereof


(57) A target substance capturing body comprises a substrate and a protein set on the surface of the substrate. The surface of the substrate comprises a portion formed of gold, and the protein comprises a first domain and a second domain. The first domain comprises at least an antibody heavy chain variable region (VH) or an antibody light chain variable region (VL), wherein the antibody heavy chain variable region (VH) or the antibody light chain variable region (VL) comprises a region specifically binding to gold of the substrate at KD = 3.0 × 10-7 M or less. Further, the second domain comprises a region specifically binding to a target substance. The protein is set on the substrate in a form that the region of the first domain specifically binding to gold binds to the portion formed of gold of the surface of the substrate.