(19)
(11)EP 2 399 588 B1

(12)EUROPEAN PATENT SPECIFICATION

(45)Mention of the grant of the patent:
29.04.2020 Bulletin 2020/18

(21)Application number: 11178613.3

(22)Date of filing:  18.01.2002
(51)International Patent Classification (IPC): 
A61K 31/7068(2006.01)
C07H 19/10(2006.01)
A61P 31/14(2006.01)
C07H 19/06(2006.01)
C07H 19/12(2006.01)

(54)

NUCLEOSIDE DERIVATIVES AS INHIBITORS OF RNA-DEPENDENT RNA VIRAL POLYMERASE

NUCLEOSIDDERIVATE ALS HEMMER VON RNA-ABBHÄNGIGEN VIRALEN RNAV-POLYMERASEN

DÉRIVÉS DE NUCLÉOSIDES EN TANT QU'INHIBITEURS DE POLYMÉRASE VIRALE D'ARN DÉPENDANTE DE L'ARN


(84)Designated Contracting States:
AT BE CH CY DE DK ES FI FR GB GR IE IT LI LU MC NL PT SE TR
Designated Extension States:
AL LT LV MK RO SI

(30)Priority: 22.01.2001 US 263313 P
06.04.2001 US 282069 P
19.06.2001 US 299320 P
25.10.2001 US 344528 P

(43)Date of publication of application:
28.12.2011 Bulletin 2011/52

(62)Application number of the earlier application in accordance with Art. 76 EPC:
02709095.0 / 1539188

(73)Proprietors:
  • Merck Sharp & Dohme Corp.
    Rahway, NJ 07065-0907 (US)
  • Ionis Pharmaceuticals, Inc.
    Carlsbad, CA 92010 (US)

(72)Inventors:
  • Carroll, Steven S
    Rahway, NJ 07065-0907 (US)
  • Lafemina, Robert L
    Rahway, NJ 07065-0907 (US)
  • Hall, Dawn L
    Rahway, NJ 07065-0907 (US)
  • Himmelberger, Amy L
    Rahway, NJ 07065-0907 (US)
  • Kuo, Lawrence C
    Rahway, NJ 07065-0907 (US)
  • MacCoss, Malcolm
    Rahway, NJ 07065-0907 (US)
  • Olsen, David B
    Rahway, NJ 07065-0907 (US)
  • Rutkowski, Carrie A
    Rahway, NJ 07065-0907 (US)
  • Tomassini, Joanne E
    Rahway, NJ 07065-0907 (US)
  • An, Haoyun
    Carlsbad, CA 92008 (US)
  • Bhat, Balkrishen
    Carlsbad, CA 92008 (US)
  • Bhat, Neelima
    Carlsbad, CA 92008 (US)
  • Cook, Phillip Dan
    Carlsbad, CA 92008 (US)
  • Eldrup, Anne B
    Carlsbad, CA 92008 (US)
  • Guinosso, Charles J
    Carlsbad, CA 92008 (US)
  • Prhavc, Marija
    Carlsbad, CA 92008 (US)
  • Prakash, Thazha P.
    Carlsbad, CA 92008 (US)

(74)Representative: Horgan, James Michael Frederic 
Merck & Co., Inc. European Patent Department Merck Sharpe & Dohme Limited Hertford Road
Hoddesdon Hertfordshire EN11 9BU
Hoddesdon Hertfordshire EN11 9BU (GB)


(56)References cited: : 
WO-A1-93/12132
WO-A2-01/90121
WO-A1-99/43691
US-A- 6 063 628
  
  • GABRIELSEN B ET AL: "IN VITRO AND IN VIVO ANTIVIRAL (RNA) EVALUATION OF OROTIDINE 5'-MONOPHOSPHATE DECARBOXYLASE INHIBITORS AND INHIBITORS AND ANALOGUES INCLUDING 6-AZAURIDINE-5'-(ETHYL METHOXYALANINYL) PHOSPHATE (A 5'-MONOPHOSPHATE PRODRUG", ANTIVIRAL CHEMISTRY & CHEMOTHERAPY, BLACKWELL SCIENTIFIC PUBL., LONDON, GB, vol. 5, no. 4, 1 January 1994 (1994-01-01) , pages 209-220, XP000645550, ISSN: 0956-3202
  • LEFEBVRE I ET AL: "Mononucleoside phosphotriester derivatives with S-acyl-2-thioethyl bioreversible phosphate-protecting groups: intracellular delivery of 3'-azido-2',3'-dideoxythymidine 5'-monophosphate", JOURNAL OF MEDICINAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 38, no. 20, 29 September 1995 (1995-09-29), pages 3941-3950, XP002293734, ISSN: 0022-2623, DOI: 10.1021/JM00020A007
  • Michael Von Büren ET AL: "Branched oligodeoxynucleotides: Automated synthesis and triple helical hybridization studie", Tetrahedron, vol. 51, no. 31, 1 July 1995 (1995-07-01), pages 8491-8506, XP55099254, ISSN: 0040-4020, DOI: 10.1016/0040-4020(95)00452-E
  • WOLFE M S ET AL: "A Concise Synthesis of 2@?-C-Methylribonucleosides", TETRAHEDRON LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 36, no. 42, 16 October 1995 (1995-10-16), pages 7611-7614, XP004027097, ISSN: 0040-4039, DOI: 10.1016/0040-4039(95)01635-U
  
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).


Description

FIELD OF THE INVENTION



[0001] The present invention provides nucleoside derivatives which are inhibitors of hepatitis C virus (HCV) NS5B polymerase, as inhibitors of HCV replication, and for the treatment of hepatitis C infection.

BACKGROUND OF THE INVENTION



[0002] Hepatitis C virus (HCV) infection is a major health problem that leads to chronic liver disease, such as cirrhosis and hepatocellular carcinoma, in a substantial number of infected individuals, estimated to be 2-15% of the world's population. There are an estimated 4.5 million infected people in the United States alone, according to the U.S. Center for Disease Control. According to the World Health Organization, there are more than 200 million infected individuals worldwide, with at least 3 to 4 million people being infected each year. Once infected, about 20% of people clear the virus, but the rest harbor HCV the rest of their lives. Ten to twenty percent of chronically infected individuals eventually develop liver-destroying cirrhosis or cancer. The viral disease is transmitted parenterally by contaminated blood and blood products, contaminated needles, or sexually and vertically from infected mothers or carrier mothers to their off-spring. Current treatments for HCV infection, which are restricted to immunotherapy with recombinant interferon-a alone or in combination with the nucleoside analog ribavirin, are of limited clinical benefit. Moreover, there is no established vaccine for HCV. Consequently, there is an urgent need for improved therapeutic agents that effectively combat chronic HCV infection. The state of the art in the treatment of HCV infection has been reviewed, and reference is made to the following publications: B. Dymock, et al., "Novel approaches to the treatment of hepatitis C virus infection," Antiviral Chemistry & Chemotherapy, 11: 79-96 (2000); H. Rosen, et al., "Hepatitis C virus: current understanding and prospects for future therapies," Molecular Medicine Today, 5: 393-399 (1999); D. Moradpour, et al., "Current and evolving therapies for hepatitis C," European J. Gastroenterol. Hepatol., 11: 1189-1202 (1999); R. Bartenschlager, "Candidate Targets for Hepatitis C Virus-Specific Antiviral Therapy," Intervirology, 40: 378-393 (1997); G.M. Lauer and B.D. Walker, "Hepatitis C Virus Infection," N. Engl. J. Med., 345: 41-52 (2001); B.W. Dymock, "Emerging therapies for hepatitis C virus infection," Emerging Drugs, 6: 13-42 (2001); C. Crabb, "Hard-Won Advances Spark Excitement about Hepatitis C," Science: 506-507 (2001); and WO99/43691 disclosing compounds potentially useful in the treatment of HBV, HCV, HIV and abnormal cellular proliferation, including tumors and cancer.

[0003] Different approaches to HCV therapy have been taken, which include the inhibition of viral serine proteinase (NS3 protease), helicase, and RNA-dependent RNA polymerase (NS5B), and the development of a vaccine.

[0004] The HCV virion is an enveloped positive-strand RNA virus with a single oligoribonucleotide genomic sequence of about 9600 bases which encodes a polyprotein of about 3,010 amino acids. The protein products of the HCV gene consist of the structural proteins C, E1, and E2, and the non-structural proteins NS2, NS3, NS4A and NS4B, and NS5A and NS5B. The nonstructural (NS) proteins are believed to provide the catalytic machinery for viral replication. The NS3 protease releases NS5B, the RNA-dependent RNA polymerase from the polyprotein chain. HCV NS5B polymerase is required for the synthesis of a double-stranded RNA from a single-stranded viral RNA that serves as a template in the replication cycle of HCV. NS5B polymerase is therefore considered to be an essential component in the HCV replication complex [see K. Ishi, et al., "Expression of Hepatitis C Virus NS5B Protein: Characterization of Its RNA Polymerase Activity and RNA Binding," Hepatology, 29: 1227-1235 (1999) and V. Lohmann, et al., "Biochemical and Kinetic Analyses of NS5B RNA-Dependent RNA Polymerase of the Hepatitis C Virus," Virology, 249: 108-118 (1998)]. Inhibition of HCV NS5B polymerase prevents formation of the double-stranded HCV RNA and therefore constitutes an attractive approach to the development of HCV-specific antiviral therapies.

[0005] It has now been found that nucleoside compounds of the present invention and certain derivatives thereof are potent inhibitors of HCV replication. The 5'-triphosphate derivatives of the nucleoside compounds are inhibitors of HCV NS5B polymerase. The instant nucleoside compounds and derivatives thereof are useful to treat HCV infection.

[0006] It is therefore an object of the present invention to provide nucleoside compounds and certain derivatives thereof which are useful as inhibitors of HCV NS5B polymerase.

[0007] It is another object of the present invention to provide nucleoside derivatives which are useful as inhibitors of the replication of hepatitis C virus.

[0008] It is another object of the present invention to provide nucleoside compounds and certain derivatives which are useful in the treatment of HCV infection.

[0009] It is another object of the present invention to provide pharmaceutical compositions comprising the novel compounds of the present invention in association with a pharmaceutically acceptable carrier.

[0010] It is another object of the present invention to provide pharmaceutical compositions comprising the nucleoside compounds and derivatives thereof for use as inhibitors of HCV NS5B polymerase.

[0011] It is another object of the present invention to provide pharmaceutical compositions comprising the nucleoside compounds and derivatives thereof for use as inhibitors of HCV replication.

[0012] It is another object of the present invention to provide pharmaceutical compositions comprising the nucleoside compounds and derivatives thereof for use in the treatment of HCV infection.

[0013] It is another object of the present invention to provide pharmaceutical compositions comprising the nucleoside compounds and derivatives thereof in combination with other agents active against HCV.

[0014] It is another object of the present invention to provide nucleoside compounds and certain derivatives thereof and their pharmaceutical compositions for use in the inhibition of HCV NS5B polymerase.

[0015] It is another object of the present invention to provide nucleoside compounds and certain derivatives thereof and their pharmaceutical compositions for use in the inhibition of HCV replication.

[0016] It is another object of the present invention to provide nucleoside compounds and certain derivatives thereof and their pharmaceutical compositions for use in the treatment of HCV infection.

[0017] It is another object of the present invention to provide nucleoside compounds and certain derivatives thereof and their pharmaceutical compositions for use in the treatment of HCV infection in combination with other agents active against HCV.

[0018] It is another object of the present invention to provide nucleoside compounds and certain derivatives thereof and their pharmaceutical compositions for use as a medicament for the inhibition of HCV replication and/or the treatment of HCV infection.

[0019] It is another object of the present invention to provide for the use of the nucleoside compounds and certain derivatives thereof of the present invention and their pharmaceutical compositions for the manufacture of a medicament for the inhibition of HCV replication and/or the treatment of HCV infection.

[0020] These and other objects will become readily apparent from the detailed description which follows.

SUMMARY OF THE INVENTION



[0021] The present invention provides the use of a compound of structural formula I which is of the stereochemical configuration:

or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for inhibiting HCV NS5B polymerase, inhibiting HCV viral replication or treating HCV infection; wherein B is

L is CH or N;

E is N or CR5;

W is O or S;

Y is H, C1-10 alkylcarbonyl, P3O9H4, P2O6H3, or P(O)R9R10;

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-4 alkyl, optionally substituted with 1 to 3 fluorine atoms,

C1-10 alkoxy, optionally substituted with C1-3 alkoxy or 1 to 3 fluorine atoms,

C2-6 alkenyloxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino;

R4 and R6 are each independently H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3; R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen; R12 and R13 are each independently hydrogen, methyl, hydroxymethyl, or fluoromethyl; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)C1-4 alkyl, OCH2O(C=O)OC1-4 alkyl, NHCHMeCO2Me, OCH(C1-4 alkyl)O(C=O)C1-4 alkyl,

wherein the compound is administered in the form of a tablet or capsule containing at least 0.1 percent by weight of the compound.



[0022] The present invention also provides novel compounds of structural formula IV of the indicated stereochemical configuration:

or a pharmaceutically acceptable salt thereof;
wherein B is

L is CH or N;

E is N or CR5;

W is O or S;

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-4 alkyl, optionally substituted with 1 to 3 fluorine atoms,

C1-10 alkoxy, optionally substituted with C1-3 alkoxy or 1 to 3 fluorine atoms,

C2-6 alkenyloxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino;

R4 and R6 are each independently H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3; R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen; R12 and R13 are each independently hydrogen, methyl, hydroxymethyl, or fluoromethyl; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)C1-4 alkyl, OCH2O(C=O)OC1-4 alkyl, NHCHMeCO2Me, OCH(C1-4 alkyl)O(C=O)C1-4 alkyl,

provided that at least one of R9 and R10 is not hydroxy.



[0023] Also encompassed within the present invention are pharmaceutical compositions containing the compounds alone or in combination with other agents active against HCV.

DETAILED DESCRIPTION OF THE INVENTION



[0024] The present invention provides the use of a compound of structural formula I which is of the stereochemical configuration:

or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for inhibiting HCV NS5B polymerase, inhibiting HCV viral replication or treating HCV infection; wherein B is

L is CH or N;

E is N or CR5;

W is O or S;

Y is H, C1-10 alkylcarbonyl, P3O9H4, P2O6H3, or P(O)R9R10;

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-4 alkyl, optionally substituted with 1 to 3 fluorine atoms,

C1-10 alkoxy, optionally substituted with C1-3 alkoxy or 1 to 3 fluorine atoms,

C2-6 alkenyloxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino;

R4 and R6 are each independently H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3; R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen; R12 and R13 are each independently hydrogen, methyl, hydroxymethyl, or fluoromethyl; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)C1-4 alkyl, OCH2O(C=O)OC1-4 alkyl, NHCHMeCO2Me, OCH(C1-4 alkyl)O(C=O)C1-4 alkyl,

wherein the compound is administered in the form of a tablet or capsule containing at least 0.1 percent by weight of the compound.



[0025] In one embodiment of the present invention, the compound is of structural formula II which is of the stereochemical configuration:

wherein B is

E is N or C-R5;

W is O or S;

Y is H, C1-10 alkylcarbonyl, P3O9H4, or P(O)R9R10;

R1 is CF3, or C1-4 alkyl and one of R2 and R3 is OH or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-3 alkyl,

trifluoromethyl,

C1-4 alkoxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino;

R4 and R6 are each independently H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3; R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen; R12 and R13 are each independently hydrogen or methyl; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)C1-4 alkyl, or OCH2O(C=O)C1-4 alkyl.



[0026] In a second embodiment of the present invention, the compound is of structural formula III which is of the stereochemical configuration:

wherein B is

W is O or S;

Y is H, C1-10 alkylcarbonyl, P3O9H4, P2O6H3, or P(O)R9R10;

R1 is CF3, or C1-4 alkyl and one of R2 and R3 is OH or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

fluoro,

C1-3 alkyl,

trifluoromethyl,

C1-8 alkylcarbonyloxy,

C1-3 alkoxy, and

amino;

R6 is H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino,

C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3;

R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)t-butyl, or OCH2O(C=O)iPr.



[0027] Illustrative of the invention is the compound 2'-C-methyl-cytidine, and the corresponding 5'-triphosphates, 5'-[bis(isopropyloxycarbonyloxymethyl)] monophosphates, 5'-mono-(S-C1-4 alkanoyl-2-thioethyl)monophosphates, and 5'-bis-(S-C1-4 alkanoyl-2-thioethyl)monophosphates thereof; or a pharmaceutically acceptable salt thereof.

[0028] Further illustrative of the invention is the compound 2'-C-methyl-cytidine, and the corresponding 5'-triphosphates, 5'-[bis(isopropyloxycarbonyloxymethyl)]monophosphates, 5'-mono-(S-pivaloyl-2-thioethyl)monophosphates, and 5'-bis-(S-pivaloyl-2-thioethyl)monophosphates thereof; or a pharmaceutically acceptable salt thereof.

[0029] Even further illustrative of the present invention is the compound 2'-C-methyl-cytidine, and the corresponding 5'-triphosphates thereof; or a pharmaceutically acceptable salt thereof.

[0030] Yet further illustrative of the invention is the compound 2'-C-methylcytidine, or a pharmaceutically acceptable salt thereof.

[0031] The present invention also provides novel compounds of structural formula IV of the indicated stereochemical configuration:

or a pharmaceutically acceptable salt thereof;
wherein B is

L is CH or N;

E is N or CR5;

W is O or S;

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-4 alkyl, optionally substituted with 1 to 3 fluorine atoms,

C1-10 alkoxy, optionally substituted with C1-3 alkoxy or 1 to 3 fluorine atoms,

C2-6 alkenyloxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino;

R4 and R6 are each independently H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3;

R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen; R12 and R13 are each independently hydrogen, methyl, hydroxymethyl, or fluoromethyl; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)C1-4 alkyl, OCH2O(C=O)OC1-4 alkyl, NHCHMeCO2Me, OCH(C1-4 alkyl)O(C=O)C1-4 alkyl,

provided that at least one of R9 and R10 is not hydroxy.



[0032] In one embodiment, there are provided novel compounds of structural formula V which are of the stereochemical configuration:

wherein B is

W is O or S;

E is N or C-R5;

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-3 alkyl,

trifluoromethyl,

C1-4 alkoxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino;

R4 and R6 are each independently H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3; R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)C1-4 alkyl, or OCH2O(C=O)C1-4 alkyl, provided that at least one of R9 and R10 is not hydroxy.



[0033] In a second embodiment, there are provided novel compounds of structural formula VI:

wherein B is

W is O or S;

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

fluoro,

C1-3 alkyl,

trifluoromethyl,

C1-3 alkoxy,

C1-8 alkylcarbonyloxy, and

amino;

R6 is H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3;

R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)t-butyl, or OCH2O(C=O)iPr, provided that at least one of R9 and R10 is not hydroxy.



[0034] Throughout the instant application, the following terms have the indicated meanings:
The alkyl groups specified above are intended to include those alkyl groups of the designated length in either a straight or branched configuration. Exemplary of such alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, sec-butyl, tertiary butyl, pentyl, isopentyl, hexyl, and isohexyl.

[0035] The term "alkenyl" shall mean straight or branched chain alkenes of two to six total carbon atoms, or any number within this range (e.g., ethenyl, propenyl, butenyl, pentenyl, etc.).

[0036] The term "alkynyl" shall mean straight or branched chain alkynes of two to six total carbon atoms, or any number within this range (e.g., ethynyl, propynyl, butynyl, pentynyl, etc.).

[0037] The term "cycloalkyl" shall mean cyclic rings of alkanes of three to eight total carbon atoms, or any number within this range (i.e., cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, or cyclooctyl).

[0038] The term "cycloheteroalkyl" is intended to include non-aromatic heterocycles containing one or two heteroatoms selected from nitrogen, oxygen and sulfur. Examples of 4-6-membered cycloheteroalkyl include azetidinyl, pyrrolidinyl, piperidinyl, morpholinyl, thiamorpholinyl, imidazolidinyl, tetrahydrofuranyl, tetrahydropyranyl, tetrahydrothiophenyl, and piperazinyl.

[0039] The term "alkoxy" refers to straight or branched chain alkoxides of the number of carbon atoms specified (e.g., C1-4 alkoxy), or any number within this range [i.e., methoxy (MeO-), ethoxy, isopropoxy, etc.].

[0040] The tenn "alkylthio" refers to straight or branched chain alkylsulfides of the number of carbon atoms specified (e.g., C1-4 alkylthio), or any number within this range [i.e., methylthio (MeS-), ethylthio, isopropylthio, etc.].

[0041] The term "alkylamino" refers to straight or branched alkylamines of the number of carbon atoms specified (e.g., C1-4 alkylamino), or any number within this range [i.e., methylamino, ethylamino, isopropylamino, t-butylamino, etc.].

[0042] The term "alkylsulfonyl" refers to straight or branched chain alkylsulfones of the number of carbon atoms specified (e.g., C1-6 alkylsulfonyl), or any number within this range [i.e., methylsulfonyl (MeSO2-), ethylsulfonyl, isopropylsulfonyl, etc.].

[0043] The term "alkyloxycarbonyl" refers to straight or branched chain esters of a carboxylic acid derivative of the present invention of the number of carbon atoms specified (e.g., C1-4 alkyloxycarbonyl), or any number within this range [i.e., methyloxycarbonyl (MeOCO-), ethyloxycarbonyl, or butyloxycarbonyl].

[0044] The term "aryl" includes both phenyl, naphthyl, and pyridyl. The aryl group is optionally substituted with one to three groups independently selected from C1-4 alkyl, halogen, cyano, nitro, trifluoromethyl, C1-4 alkoxy, and C1-4 alkylthio.

[0045] The term "halogen" is intended to include the halogen atoms fluorine, chlorine, bromine and iodine.

[0046] The term "substituted" shall be deemed to include multiple degrees of substitution by a named substituent. Where multiple substituent moieties are disclosed or claimed, the substituted compound can be independently substituted by one or more of the disclosed or claimed substituent moieties, singly or plurally.

[0047] The term "5'-triphosphate" refers to a triphosphoric acid ester derivative of the 5'-hydroxyl group of a nucleoside compound of the present invention having the following general structural formula VII:

wherein B, R1-R4, R12, and R13 are as defined above. The compounds of the present invention are also intended to include pharmaceutically acceptable salts of the triphosphate ester as well as pharmaceutically acceptable salts of 5'-monophosphate and 5'-diphosphate ester derivatives of the structural formulae VIII and IX, respectively,



[0048] The term "5'-(S-acyl-2-thioethyl)phosphate" or "SATE" refers to a mono- or di-ester derivative of a 5'-monophosphate nucleoside of the present invention of structural formulae X and XI, respectively, as well as pharmaceutically acceptable salts of the mono-ester,





[0049] The term "composition", as in "pharmaceutical composition," is intended to encompass a product comprising the active ingredient(s) and the inert ingredient(s) that make up the carrier, as well as any product which results, directly or indirectly, from combination, complexation or aggregation of any two or more of the ingredients, or from dissociation of one or more of the ingredients, or from other types of reactions or interactions of one or more of the ingredients. Accordingly, the pharmaceutical compositions of the present invention encompass any composition made by admixing a compound of the present invention and a pharmaceutically acceptable carrier.

[0050] The terms "administration of" and "administering a" compound should be understood to mean providing a compound of the invention to the individual in need.

[0051] Another aspect of the present invention is concerned with a compound of the present invention for use in inhibiting HCV NS5B polymerase, inhibiting HCV replication, or treating HCV infection, wherein the compound of the present invention is used in combination with one or more agents useful for treating HCV infection. Such agents active against HCV include ribavirin, levovirin, viramidine, thymosin alpha-1, interferon-α, pegylated interferon-α (peginterferon-α), a combination of interferon-α and ribavirin, a combination of peginterferon-α and ribavirin, a combination of interferon-a and levovirin, and a combination of peginterferon-α and levovirin. Interferon-a includes recombinant interferon-α2a (such as Roferon interferon available from Hoffmann-LaRoche, Nutley, NJ), pegylated interferon-α2a (Pegasys™), interferon-α2b (such as Intron-A interferon available from Schering Corp., Kenilworth, NJ), pegylated interferon-α2b (PegIntron™), a recombinant consensus interferon (such as interferon alphacon-1), and a purified interferon-a product. Amgen's recombinant consensus interferon has the brand name Infergen®. Levovirin is the L-enantiomer of ribavirin which has shown immunomodulatory activity similar to ribavirin. Viramidine is an amidino analog of ribavirin disclosed in WO 01/60379 (assigned to ICN Pharmaceuticals). In accordance with the present invention, the individual components of the combination can be administered separately at different times during the course of therapy or concurrently in divided or single combination forms. The instant invention is therefore to be understood as embracing all such regimes of simultaneous or alternating treatment, and the term "administering" is to be interpreted accordingly. It will be understood that the scope of combinations of the compounds of this invention with other agents useful for treating HCV infection includes in principle any combination with any pharmaceutical composition for treating HCV infection. When a compound of the present invention or a pharmaceutically acceptable salt thereof is used in combination with a second therapeutic agent active against HCV, the dose of each compound may be either the same as or different from the dose when the compound is used alone.

[0052] For the treatment of HCV infection, the compounds of the present invention may also be administered in combination with an agent that is an inhibitor of HCV NS3 serine protease, such as LY570310 (VX-950). HCV NS3 serine protease is an essential viral enzyme and has been described to be an excellent target for inhibition of HCV replication. Both substrate and non-substrate based inhibitors of HCV NS3 protease inhibitors are disclosed in WO 98/17679, WO 98/22496, WO 98/46630, WO 99/07733, WO 99/07734, WO 99/38888, WO 99/50230, WO 99/64442, WO 00/09543, WO 00/59929, WO 01/74768, WO 01/81325, and GB-2337262. HCV NS3 protease as a target for the development of inhibitors of HCV replication and for the treatment of HCV infection is discussed in B.W. Dymock, "Emerging therapies for hepatitis C virus infection," Emerging Drugs, 6: 13-42 (2001).

[0053] Ribavirin, levovirin, and viramidine may exert their anti-HCV effects by modulating intracellular pools of guanine nucleotides via inhibition of the intracellular enzyme inosine monophosphate dehydrogenase (IMPDH). IMPDH is the rate-limiting enzyme on the biosynthetic route in de novo guanine nucleotide biosynthesis. Ribavirin is readily phosphorylated intracellularly and the monophosphate derivative is an inhibitor of IMPDH. Thus, inhibition of IMPDH represents another useful target for the discovery of inhibitors of HCV replication. Therefore, the compounds of the present invention may also be administered in combination with an inhibitor of IMPDH, such as VX-497, which is disclosed in WO 97/41211 and WO 01/00622, (assigned to Vertex); another IMPDH inhibitor, such as that disclosed in WO 00/25780 (assigned to Bristol-Myers Squibb); or mycophenolate mofetil [see A.C. Allison and E.M. Eugui, Agents Action, 44 (Suppl.): 165 (1993)].

[0054] For the treatment of HCV infection, the compounds of the present invention may also be administered in combination with the antiviral agent amantadine (1-aminoadamantane) [for a comprehensive description of this agent, see J. Kirschbaum, Anal. Profiles Drug Subs. 12: 1-36 (1983)].

[0055] The compounds of the present invention may also be combined for the treatment of HCV infection with antiviral 2'-C-branched ribonucleosides disclosed in R. E. Harry-O'kuru, et al., J. Org. Chem., 62: 1754-1759 (1997); M. S. Wolfe, et al., Tetrahedron Lett., 36: 7611-7614 (1995); and U.S. Patent No. 3,480,613 (Nov. 25, 1969). Such 2'-C-branched ribonucleosides include 2'-C-methyl-cytidine, 2'-C-methyl-adenosine, 2'-C-methyl-guanosine, and 9-(2-C-methyl-β-D-ribofuranosyl)-2,6-diaminopurine.

[0056] By "pharmaceutically acceptable" is meant that the carrier, diluent, or excipient must be compatible with the other ingredients of the formulation and not deleterious to the recipient thereof.

[0057] Also included within the present invention are pharmaceutical compositions comprising the novel nucleoside compounds and derivatives thereof of the present invention in association with a pharmaceutically acceptable carrier. Another example of the invention is a pharmaceutical composition made by combining any of the compounds described above and a pharmaceutically acceptable carrier. Another illustration of the invention is a process for making a pharmaceutical composition comprising combining any of the compounds described above and a pharmaceutically acceptable carrier.

[0058] Also included within the present invention are pharmaceutical compositions useful for inhibiting HCV NS5B polymerase comprising an effective amount of a compound of this invention and a pharmaceutically acceptable carrier. Pharmaceutical compositions useful for treating HCV infection are also encompassed by the present invention as well as compounds of the present invention for use in inhibiting HCV NS5B polymerase and for use in treating HCV replication. Additionally, the present invention is directed to a pharmaceutical composition comprising a therapeutically effective amount of a compound of the present invention in combination with a therapeutically effective amount of another agent active against HCV. Agents active against HCV include ribavirin, levovirin, viramidine, thymosin alpha-1, an inhibitor of HCV NS3 serine protease, interferon-a, pegylated interferon-a (peginterferon-α), a combination of interferon-a and ribavirin, a combination of peginterferon-α and ribavirin, a combination of interferon-a and levovirin, and a combination of peginterferon-α and levovirin. Interferon-a includes recombinant interferon-α2a (such as Roferon interferon available from Hoffmann-LaRoche, Nutley, NJ), interferon-α2b (such as Intron-A interferon available from Schering Corp., Kenilworth, NJ), a consensus interferon, and a purified interferon-a product. For a discussion of ribavirin and its activity against HCV, see J.O. Saunders and S.A. Raybuck, "Inosine Monophosphate Dehydrogenase: Consideration of Structure, Kinetics, and Therapeutic Potential," Ann. Rep. Med. Chem., 35: 201-210 (2000).

[0059] Another aspect of the present invention provides for the use of nucleoside compounds and derivatives thereof and their pharmaceutical compositions for the manufacture of a medicament for the inhibition of HCV replication, and/or the treatment of HCV infection. Yet a further aspect of the present invention provides for nucleoside compounds and derivatives thereof and their pharmaceutical compositions for use as a medicament for the inhibition of HCV replication, and/or for the treatment of HCV infection.

[0060] The pharmaceutical compositions of the present invention comprise a compound of structural formula I or IV as an active ingredient or a pharmaceutically acceptable salt thereof, and may also contain a pharmaceutically acceptable carrier and optionally other therapeutic ingredients.

[0061] The compositions include compositions suitable for oral, rectal, topical, parenteral (including subcutaneous, intramuscular, and intravenous), ocular (ophthalmic), pulmonary (nasal or buccal inhalation), or nasal administration, although the most suitable route in any given case will depend on the nature and severity of the conditions being treated and on the nature of the active ingredient. They may be conveniently presented in unit dosage form and prepared by any of the methods well-known in the art of pharmacy.

[0062] In practical use, the compounds of structural formulae I and IV can be combined as the active ingredient in intimate admixture with a pharmaceutical carrier according to conventional pharmaceutical compounding techniques. The carrier may take a wide variety of forms depending on the form of preparation desired for administration, e.g., oral or parenteral (including intravenous). In preparing the compositions for oral dosage form, any of the usual pharmaceutical media may be employed, such as, for example, water, glycols, oils, alcohols, flavoring agents, preservatives, and coloring agents in the case of oral liquid preparations, such as, for example, suspensions, elixirs and solutions; or carriers such as starches, sugars, microcrystalline cellulose, diluents, granulating agents, lubricants, binders, and disintegrating agents in the case of oral solid preparations such as, for example, powders, hard and soft capsules and tablets, with the solid oral preparations being preferred over the liquid preparations.

[0063] Because of their ease of administration, tablets and capsules represent the most advantageous oral dosage unit form in which case solid pharmaceutical carriers are obviously employed. If desired, tablets may be coated by standard aqueous or nonaqueous techniques. Such compositions and preparations should contain at least 0.1 percent of active compound. The percentage of active compound in these compositions may, of course, be varied and may conveniently be between about 2 percent to about 60 percent of the weight of the unit. The amount of active compound in such therapeutically useful compositions is such that an effective dosage will be obtained. The active compounds can also be administered intranasally as, for example, liquid drops or spray.

[0064] The tablets, pills, and capsules may also contain a binder such as gum tragacanth, acacia, corn starch or gelatin; excipients such as dicalcium phosphate; a disintegrating agent such as corn starch, potato starch or alginic acid; a lubricant such as magnesium stearate; and a sweetening agent such as sucrose, lactose or saccharin. When a dosage unit form is a capsule, it may contain, in addition to materials of the above type, a liquid carrier such as a fatty oil.

[0065] Various other materials may be present as coatings or to modify the physical form of the dosage unit. For instance, tablets may be coated with shellac, sugar or both. A syrup or elixir may contain, in addition to the active ingredient, sucrose as a sweetening agent, methyl and propylparabens as preservatives, a dye and a flavoring such as cherry or orange flavor.

[0066] Compounds of structural formulae I and IV may also be administered parenterally. Solutions or suspensions of these active compounds can be prepared in water suitably mixed with a surfactant such as hydroxy-propylcellulose. Dispersions can also be prepared in glycerol, liquid polyethylene glycols and mixtures thereof in oils. Under ordinary conditions of storage and use, these preparations contain a preservative to prevent the growth of microorganisms.

[0067] The pharmaceutical forms suitable for injectable use include sterile aqueous solutions or dispersions and sterile powders for the extemporaneous preparation of sterile injectable solutions or dispersions. In all cases, the form must be sterile and must be fluid to the extent that easy syringability exists. It must be stable under the conditions of manufacture and storage and must be preserved against the contaminating action of microorganisms such as bacteria and fungi. The carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (e.g. glycerol, propylene glycol and liquid polyethylene glycol), suitable mixtures thereof, and vegetable oils.

[0068] Any suitable route of administration may be employed for providing a mammal, especially a human with an effective dosage of a compound of the present invention. For example, oral, rectal, topical, parenteral, ocular, pulmonary, and nasal may be employed. Dosage forms include tablets, troches, dispersions, suspensions, solutions, capsules, creams, ointments, and aerosols. Preferably compounds of structural formulae I and IV are administered orally.

[0069] For oral administration to humans, the dosage range is 0.01 to 1000 mg/kg body weight in divided doses. In one embodiment the dosage range is 0.1 to 100 mg/kg body weight in divided doses. In another embodiment the dosage range is 0.5 to 20 mg/kg body weight in divided doses. For oral administration, the compositions are preferably provided in the form of tablets or capsules containing 1.0 to 1000 milligrams of the active ingredient, particularly, 1, 5, 10, 15, 20, 25, 50, 75, 100, 150, 200, 250, 300, 400, 500, 600, 750, 800, 900, and 1000 milligrams of the active ingredient for the symptomatic adjustment of the dosage to the patient to be treated.

[0070] The effective dosage of active ingredient employed may vary depending on the particular compound employed, the mode of administration, the condition being treated and the severity of the condition being treated. Such dosage may be ascertained readily by a person skilled in the art. This dosage regimen may be adjusted to provide the optimal therapeutic response.

[0071] The compounds of the present invention contain one or more asymmetric centers and can thus occur as racemates and racemic mixtures, single enantiomers, diastereomeric mixtures and individual diastereomers. The present invention is meant to comprehend nucleoside derivatives having the β-D stereochemical configuration for the five-membered furanose ring as depicted in the structural formula below, that is, nucleoside compounds in which the substituents at C-1 and C-4 of the five-membered furanose ring have the β-stereochemical configuration ("up" orientation as denoted by a bold line).



[0072] The stereochemistry of the substituents at the C-2 and C-3 positions of the furanose ring of the compounds of the present invention is denoted either by a dashed line which signifies that the substituent, for example R2 in structural formula VI, has the α (substituent "down") configuration or a squiggly line which signifies that the substituent, for example R3 in structural formula VI, can have either the α (substituent "down") or β (substituent "up") configuration.

[0073] Some of the compounds described herein contain olefinic double bonds, and unless specified otherwise, are meant to include both E and Z geometric isomers.

[0074] Some of the compounds described herein may exist as tautomers such as keto-enol tautomers. The individual tautomers as well as mixtures thereof are encompassed with compounds of structural formulae I and IV.

[0075] Compounds of structural formulae I and IV may be separated into their individual diastereoisomers by, for example, fractional crystallization from a suitable solvent, for example methanol or ethyl acetate or a mixture thereof, or via chiral chromatography using an optically active stationary phase.

[0076] Alternatively, any stereoisomer of a compound of the structural formulae I and IV may be obtained by stereospecific synthesis using optically pure starting materials or reagents of known configuration.

[0077] The stereochemistry of the substituents at the C-2 and C-3 positions of the furanose ring of compounds of structural formula XII (not according to the present invention) is denoted by squiggly lines which signifies that substituents Ra, Rb, Rc and Rh can have either the I (substituent "down") or ϑ (substituent "up") configuration independently of one another.



[0078] The compounds of the present invention may be administered in the form of a pharmaceutically acceptable salt. The term "pharmaceutically acceptable salt" refers to salts prepared from pharmaceutically acceptable non-toxic bases or acids including inorganic or organic bases and inorganic or organic acids. Salts of basic compounds encompassed within the term "pharmaceutically acceptable salt" refer to non-toxic salts of the compounds of this invention which are generally prepared by reacting the free base with a suitable organic or inorganic acid. Representative salts of basic compounds of the present invention include the following: acetate, benzenesulfonate, benzoate, bicarbonate, bisulfate, bitartrate, borate, bromide, camsylate, carbonate, chloride, clavulanate, citrate, dihydrochloride, edetate, edisylate, estolate, esylate, fumarate, gluceptate, gluconate, glutamate, glycollylarsanilate, hexylresorcinate, hydrabamine, hydrobromide, hydrochloride, hydroxynaphthoate, iodide, isothionate, lactate, lactobionate, laurate, malate, maleate, mandelate, mesylate, methylbromide, methylnitrate, methylsulfate, mucate, napsylate, nitrate, N-methylglucamine ammonium salt, oleate, oxalate, pamoate (embonate), palmitate, pantothenate, phosphate/diphosphate, polygalacturonate, salicylate, stearate, sulfate, subacetate, succinate, tannate, tartrate, teoclate, tosylate, triethiodide and valerate. Furthermore, where the compounds of the invention carry an acidic moiety, suitable pharmaceutically acceptable salts thereof include salts derived from inorganic bases including aluminum, ammonium, calcium, copper, ferric, ferrous, lithium, magnesium, manganic, mangamous, potassium, sodium, and zinc. Particularly preferred are the ammonium, calcium, magnesium, potassium, and sodium salts. Salts derived from pharmaceutically acceptable organic non-toxic bases include salts of primary, secondary, and tertiary amines, cyclic amines, and basic ionexchange resins, such as arginine, betaine, caffeine, choline, N,N-dibenzylethylenediamine, diethylamine, 2-diethylaminoethanol, 2-dimethylaminoethanol, ethanolamine, ethylenediamine, N-ethylmorpholine, N-ethylpiperidine, glucamine, glucosamine, histidine, hydrabamine, isopropylamine, lysine, methylglucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethylamine, trimethylamine, tripropylamine, and tromethamine.

Preparation of the Nucleoside Compounds and Derivatives of the Invention



[0079] The nucleoside compounds and derivatives thereof of the present invention can be prepared following synthetic methodologies well-established in the practice of nucleoside and nucleotide chemistry. Reference is made to the following text for a description of synthetic methods used in the preparation of the compounds of the present invention: "Chemistry of Nucleosides and Nucleotides," L.B. Townsend, ed., Vols. 1-3, Plenum Press, 1988.

[0080] A representative general method for the preparation of compounds similar to the compounds of the present invention is outlined in Scheme 1 below. This scheme illustrates the synthesis of compounds of structural formula 1-7 wherein the furanose ring has the β-D-ribo configuration. The starting material is a 3,5-bis-O-protected alkyl furanoside, such as methyl furanoside, of structural formula 1-1. The C-2 hydroxyl group is then oxidized with a suitable oxidizing agent, such as a chromium trioxide or chromate reagent or Dess-Martin periodinane, or by Swern oxidation, to afford a C-2 ketone of structural formula 1-2. Addition of a Grignard reagent, such as an alkyl, alkenyl, or alkynyl magnesium halide (for example, MeMgBr, EtMgBr, vinylMgBr, allylMgBr, and ethynylMgBr) or an alkyl, alkenyl, or alkynyl lithium, such as MeLi, across the carbonyl double bond of 1-2 in a suitable organic solvent, such as tetrahydrofuran or diethyl ether, affords the C-2 tertiary alcohol of structural formula 1-3. A good leaving group (such as Cl, Br, and I) is next introduced at the C-1 (anomeric) position of the furanose sugar derivative by treatment of the furanoside of formula 1-3 with a hydrogen halide in a suitable organic solvent, such as hydrogen bromide in acetic acid, to afford the intermediate furanosyl halide 1-4. A C-1 sulfonate, such methanesulfonate (MeSO2O-), trifluoromethanesulfonate (CF3SO2O-), or p-toluenesulfonate (-OTs), may also serve as a useful leaving group in the subsequent reaction to generate the glycosidic (nucleosidic) linkage. The nucleosidic linkage is constructed by treatment of the intermediate of structural formula 1-4 with the metal salt (such as lithium, sodium, or potassium) of an appropriately substituted 1H-pyrrolo[2,3-d]pyrimidine 1-5, such as an appropriately substituted 4-halo-1H-pyrrolo[2,3-d]pyrimidine, which can be generated in situ by treatment with an alkali hydride (such as sodium hydride), an alkali hydroxide (such as potassium hydroxide), an alkali carbonate (such as potassium carbonate), or an alkali hexamethyldisilazide (such as NaHMDS) in a suitable anhydrous organic solvent, such as acetonitrile, tetrahydrofuran, 1-methyl-2-pyrrolidinone, or N,N-dimethylformamide (DMF). The displacement reaction can be catalyzed by using a phase-transfer catalyst, such as TDA-1 or triethylbenzylammonium chloride, in a two-phase system (solid-liquid or liquid-liquid). The optional protecting groups in the protected nucleoside of structural formula 1-6 are then cleaved following established deprotection methodologies, such as those described in T.W. Greene and P.G.M. Wuts, "Protective Groups in Organic Synthesis," 3rd ed., John Wiley & Sons, 1999. Optional introduction of an amino group at the 4-position of the pyrrolo[2,3-d]pyrimidine nucleus is effected by treatment of the 4-halo intermediate 1-6 with the appropriate amine, such as alcoholic ammonia or liquid ammonia, to generate a primary amine at the C-4 position (-NH2), an alkylamine to generate a secondary amine (-NHR), or a dialkylamine to generate a tertiary amine (-NRR'). A 7H-pyrrolo[2,3-d]pyrimidin-4(3H)one compound may be derived by hydrolysis of 1-6 with aqueous base, such as aqueous sodium hydroxide. Alcoholysis (such as methanolysis) of 1-6 affords a C-4 alkoxide (-OR), whereas treatment with an alkyl mercaptide affords a C-4 alkylthio (-SR) derivative. Subsequent chemical manipulations well-known to practitioners of ordinary skill in the art of organic/medicinal chemistry may be required to attain the desired compounds.





[0081] The examples below provide citations to literature publications, which contain details for the preparation of final compounds or intermediates employed in the preparation of final compounds of the present invention. The nucleoside compounds of the present invention were prepared according to procedures detailed in the following examples. The examples are not intended to be limitations on the scope of the instant invention in any way, and they should not be so construed. Those skilled in the art of nucleoside and nucleotide synthesis will readily appreciate that known variations of the conditions and processes of the following preparative procedures can be used to prepare these and other compounds of the present invention. All temperatures are degrees Celsius unless otherwise noted.

EXAMPLE 86


Nucleoside 5'-Triphosphates



[0082] The nucleoside 5'-triphosphates of the present invention were prepared according to the general procedures described in Chem. Rev. 100: 2047 (2000).

EXAMPLE 87


Purification and Purity Analysis of Nucleoside 5-Triphosphates



[0083] Triphosphates were purified by anion exchange (AX) chromatography using a 30 x 100 mm Mono Q column (Pharmacia) with a buffer system of 50 mM Tris, pH 8. Elution gradients were typically from 40 mM NaCl to 0.8 M NaCl in two column volumes at 6.5 mL/min. Appropriate fractions from anion exchange chromatography were collected and desalted by reverse-phase (RP) chromatography using a Luna C18 250 × 21 mm column (Phenomenex) with a flow rate of 10 ml/min. Elution gradients were generally from 1% to 95% methanol in 14 min at a constant concentration of 5 mM triethylammonium acetate (TEAA).

[0084] Mass spectra of the purified triphosphates were determined using on-line HPLC mass spectrometry on a Hewlett-Packard (Palo Alto, CA) MSD 1100. A Phenomenex Luna (C18(2)), 150 × 2 mm, plus 30 x 2 mm guard column, 3-µm particle size was used for RP HPLC. A 0 to 50% linear gradient (15 min) of acetonitrile in 20 mM TEAA (triethylammonium acetate) pH 7 was performed in series with mass spectral detection in the negative ionization mode. Nitrogen gas and a pneumatic nebulizer were used to generate the electrospray. The mass range of 150-900 was sampled. Molecular masses were determined using the HP Chemstation analysis package.

[0085] The purity of the purified triphosphates was determined by analytical RP and AX HPLC. RP HPLC with a Phenomonex Luna or Jupiter column (250 × 4.6 mm), 5-µm particle size was typically run with a 2-70% acetonitrile gradient in 15 min in 100 mM TEAA, pH 7. AX HPLC was performed on a 1.6 × 5 mm Mono Q column (Pharmacia). Triphosphates were eluted with a gradient of 0 to 0.4 M NaCl at constant concentration of 50 mM Tris, pH 8. Purity of the triphosphates was generally >80%.

EXAMPLE 88


Nucleoside 5'-Monophosphates



[0086] The nucleoside 5'-monophosphates of the present invention were prepared according to the general procedure described in Tetrahedron Lett. 50: 5065 (1967).

EXAMPLE 122


2 'C-Methyl-cytidine



[0087] 



[0088] This compound was prepared following procedures described in L. Beigelman et al., Carbohyd. Res. 166: 219-232 (1987) or X-Q Tang, et al., J. Org. Chem. 64: 747-754 (1999).

BIOLOGICAL ASSAYS



[0089] The assays employed to measure the inhibition of HCV NS5B polymerase and HCV replication are described below.

[0090] The effectiveness of the compounds of the present invention as inhibitors of HCV NS5B RNA-dependent RNA polymerase (RdRp) was measured in the following assay.

A. Assay for Inhibition of HCV NS5B Polymerase:



[0091] This assay was used to measure the ability of the nucleoside derivatives of the present invention to inhibit the enzymatic activity of the RNA-dependent RNA polymerase (NS5B) of the hepatitis C virus (HCV) on a heteromeric RNA template.

Procedure:



[0092] Assay Buffer Conditions: (50 µL -total/reaction)

20 mM Tris, pH 7.5

50 µM EDTA

5 mM DTT

2 mM MgCl2

80 mM KCl

0.4 U/µL RNAsin (Promega, stock is 40 units/µL)

0.75 µg t500 (a 500-nt RNA made using T7 runoff transcription with a sequence from the NS2/3 region of the hepatitis C genome)

1.6 µg purified hepatitis C NS5B (form with 21 amino acids C-terminally truncated)

1 µM A,C,U,GTP (Nucleoside triphosphate mix)

[alpha-32P]-GTP or [alpha-33P]-GTP



[0093] The compounds were tested at various concentrations up to 100 µM final concentration.

[0094] An appropriate volume of reaction buffer was made including enzyme and template t500. Nucleoside derivatives of the present invention were pipetted into the wells of a 96-well plate. A mixture of nucleoside triphosphates (NTP's), including the radiolabeled GTP, was made and pipetted into the wells of a 96-well plate. The reaction was initiated by addition of the enzyme-template reaction solution and allowed to proceed at room temperature for 1-2 h.

[0095] The reaction was quenched by addition of 20 µL 0.5M EDTA, pH 8.0. Blank reactions in which the quench solution was added to the NTPs prior to the addition of the reaction buffer were included.

[0096] 50 pL of the quenched reaction were spotted onto DE81 filter disks (Whatman) and allowed to dry for 30 min. The filters were washed with 0.3 M ammonium formate, pH 8 (150 mL/wash until the cpm in 1 mL wash is less than 100, usually 6 washes). The filters were counted in 5-mL scintillation fluid in a scintillation counter.

[0097] The percentage of inhibition was calculated according to the following equation: % Inhibition = [1-(cpm in test reaction - cpm in blank) / (cpm in control reaction - cpm in blank)] x 100.

[0098] Representative compounds tested in the HCV NS5B polymerase assay exhibited IC50's less than 100 micromolar.

B. Assay for Inhibition of HCV RNA Replication:



[0099] The compounds of the present invention were also evaluated for their ability to affect the replication of Hepatitis C Virus RNA in cultured hepatoma (HuH-7) cells containing a subgenomic HCV Replicon. The details of the assay are described below. This Replicon assay is a modification of that described in V. Lohmann, F. Korner, J-O. Koch, U. Herian, L. Theilmann, and R. Bartenschlager, "Replication of a Sub-genomic Hepatitis C Virus RNAs in a Hepatoma Cell Line," Science 285:110 (1999).

Protocol:



[0100] The assay was an in situ Ribonuclease protection, Scintillation Proximity based-plate assay (SPA). 10,000 - 40,000 cells were plated in 100-200 µL of media containing 0.8mg/mL G418 in 96-well cytostar plates (Amersham). Compounds were added to cells at various concentrations up to 100 µM in 1% DMSO at time 0 to 18 h and then cultured for 24-96 h. Cells were fixed (20 min, 10% formalin), permeabilized (20 min, 0.25% Triton X-100/PBS) and hybridized (overnight, 50°C) with a single-stranded 33P RNA probe complementary to the (+) strand NS5B (or other genes) contained in the RNA viral genome. Cells were washed, treated with RNAse, washed, heated to 65°C and counted in a Top-Count. Inhibition of replication was read as a decrease in counts per minute (cpm).

[0101] Human HuH-7 hepatoma cells, which were selected to contain a subgenomic replicon, carry a cytoplasmic RNA consisting of an HCV 5' non-translated region (NTR), a neomycin selectable marker, an EMCV IRES (internal ribosome entry site), and HCV non-structural proteins NS3 through NS5B, followed by the 3' NTR.

[0102] Representative compounds tested in the replication assay exhibited EC50's less than 100 micromolar.

[0103] The nucleoside derivatives of the present invention were also evaluated for cellular toxicity and anti-viral specificity in the counterscreens described below.

C. COUNTERSCREENS:



[0104] The ability of the nucleoside derivatives of the present invention to inhibit human DNA polymerases was measured in the following assays.

a. Inhibition of Human DNA Polymerases alpha and beta:


Reaction Conditions:



[0105] 50 µL reaction volume

Reaction buffer components:



[0106] 20 mM Tris-HCl, pH 7.5
200 µg/mL bovine serum albumin
100 mM KCl
2 mM β-mercaptoethanol
10 mM MgCl2
1.6 µM dA, dG, dC, dTTP
α-33P-dATP

Enzyme and template:



[0107] 0.05 mg/mL gapped fish sperm DNA template
0.01 U/µL DNA polymerase α or β

Preparation of gapped fish sperm DNA template:



[0108] Add 5 µL 1M MgCl2 to 500 µL activated fish sperm DNA (USB 70076);
Warm to 37°C and add 30 µL of 65 U/µL of exonuclease III (GibcoBRL 18013-011); Incubate 5 min at 37°C;
Terminate reaction by heating to 65°C for 10 min;
Load 50-100 µL aliquots onto Bio-spin 6 chromatography columns (Bio-Rad 732-6002) equilibrated with 20 mM Tris-HCl, pH 7.5;
Elute by centrifugation at 1,000Xg for 4 min;
Pool eluate and measure absorbance at 260 nm to determine concentration.

[0109] The DNA template was diluted into an appropriate volume of 20 mM Tris-HCl, pH 7.5 and the enzyme was diluted into an appropriate volume of 20 mM Tris-HCl, containing 2 mM β-mercaptoethanol, and 100 mM KCl. Template and enzyme were pipetted into microcentrifuge tubes or a 96 well plate. Blank reactions excluding enzyme and control reactions excluding test compound were also prepared using enzyme dilution buffer and test compound solvent, respectively. The reaction was initiated with reaction buffer with components as listed above. The reaction was incubated for 1 hour at 37°C. The reaction was quenched by the addition of 20 µL 0.5M EDTA. 50 µL of the quenched reaction was spotted onto Whatman DE81 filter disks and air dried. The filter disks were repeatedly washed with 150 mL 0.3M ammonium formate, pH 8 until 1 mL of wash is < 100 cpm. The disks were washed twice with 150 mL absolute ethanol and once with 150 mL anhydrous ether, dried and counted in 5 mL scintillation fluid.

[0110] The percentage of inhibition was calculated according to the following equation: % inhibition = [1-(cpm in test reaction - cpm in blank)/(cpm in control reaction - cpm in blank)] x 100.

b. Inhibition of Human DNA Polymerase gamma :



[0111] The potential for inhibition of human DNA polymerase gamma was measured in reactions that included 0.5 ng/µL enzyme; 10 µM dATP, dGTP, dCTP, and TTP; 2 µCi/reaction [α-33P]-dATP, and 0.4 µg/µL activated fish sperm DNA (purchased from US Biochemical) in a buffer containing 20 mM Tris pH8, 2 mM β-mercaptoethanol, 50 mM KCl, 10 mM MgCl2, and 0.1 µg/µL BSA. Reactions were allowed to proceed for 1 h at 37°C and were quenched by addition of 0.5 M EDTA to a final concentration of 142 mM. Product formation was quantified by anion exchange filter binding and scintillation counting. Compounds were tested at up to 50 µM.

[0112] The percentage of inhibition was calculated according to the following equation: % inhibition = [1-(cpm in test reaction - cpm in blank)/(cpm in control reaction - cpm in blank)] x 100.

[0113] The ability of the nucleoside derivatives of the present invention to inhibit HIV infectivity and HIV spread was measured in the following assays.

c. HIV Infectivity Assay



[0114] Assays were performed with a variant of HeLa Magi cells expressing both CXCR4 and CCR5 selected for low background β-galactosidase (β-gal) expression. Cells were infected for 48 h, and β-gal production from the integrated HIV-1 LTR promoter was quantified with a chemiluminescent substrate (Galactolight Plus, Tropix, Bedford, MA). Inhibitors were titrated (in duplicate) in twofold serial dilutions starting at 100 µM; percent inhibition at each concentration was calculated in relation to the control infection.

d. Inhibition of HIV Spread



[0115] The ability of the compounds of the present invention to inhibit the spread of the human immunedeficiency virus (HIV) was measured by the method described in U.S. Patent No. 5,413,999 (May 9, 1995), and J.P.Vacca, et al., Proc. Natl. Acad. Sci., 91: 4096-4100 (1994).

[0116] The nucleoside derivatives of the present invention were also screened for cytotoxicity against cultured hepatoma (HuH-7) cells containing a subgenomic HCV Replicon in an MTS cell-based assay as described in the assay below. The HuH-7 cell line is described in H. Nakabayashi, et al., Cancer Res., 42: 3858 (1982).

e. Cytotoxicity assay:



[0117] Cell cultures were prepared in appropriate media at concentrations of approximately 1.5 x 10 cells/mL for suspension cultures in 3 day incubations and 5.0 x 104 cells/mL for adherent cultures in 3 day incubations. 99 µL of cell culture was transferred to wells of a 96-well tissue culture treated plate, and 1 µL of 100-times final concentration of the test compound in DMSO was added. The plates were incubated at 37°C and 5% CO2 for a specified period of time. After the incubation period, 20 µL of CellTiter 96 Aqueous One Solution Cell Proliferation Assay reagent (MTS) (Promega) was added to each well and the plates were incubated at 37°C and 5% CO2 for an additional period of time up to 3 h. The plates were agitated to mix well and absorbance at 490 nm was read using a plate reader. A standard curve of suspension culture cells was prepared with known cell numbers just prior to the addition of MTS reagent. Metabolically active cells reduce MTS to formazan. Formazan absorbs at 490 nm. The absorbance at 490 nm in the presence of compound was compared to absorbance in cells without any compound added.

[0118] Reference: Cory, A. H. et al., "Use of an aqueous soluble tetrazolium/formazan assay for cell growth assays in culture," Cancer Commun. 3: 207 (1991).

[0119] The following assays were employed to measure the activity of the compounds of the present invention against other RNA-dependent RNA viruses:

a. Determination of In Vitro Antiviral Activity of Compounds Against Rhinovirus (Cytopathic Effect Inhibition Assay):



[0120] Assay conditions are described in the article by Sidwell and Huffman, "Use of disposable microtissue culture plates for antiviral and interferon induction studies," Appl. Microbiol. 22: 797-801 (1971).

Viruses:



[0121] Rhinovirus type 2 (RV-2), strain HGP, was used with KB cells and media (0.1% NaHCO3, no antibiotics) as stated in the Sidwell and Huffman reference. The virus, obtained from the ATCC, was from a throat swab of an adult male with a mild acute febrile upper respiratory illness.
Rhinovirus type 9 (RV-9), strain 211, and rhinovirus type 14 (RV-14), strain Tow, were also obtained from the American Type Culture Collection (ATCC) in Rockville, MD. RV-9 was from human throat washings and RV-14 was from a throat swab of a young adult with upper respiratory illness. Both of these viruses were used with HeLa Ohio-1 cells (Dr. Fred Hayden, Univ. of VA) which were human cervical epitheloid carcinoma cells. MEM (Eagle's minimum essential medium) with 5% Fetal Bovine serum (FBS) and 0.1% NaHCO3 was used as the growth medium. Antiviral test medium for all three virus types was MEM with 5% FBS, 0.1% NaHCO3, 50 µg gentamicin/mL, and 10 mM MgCl2.
2000 µg/mL was the highest concentration used to assay the compounds of the present invention. Virus was added to the assay plate approximately 5 min after the test compound. Proper controls were also run. Assay plates were incubated with humidified air and 5% CO2 at 37°C. Cytotoxicity was monitored in the control cells microscopically for morphologic changes. Regression analysis of the virus CPE data and the toxicity control data gave the ED50 (50% effective dose) and CC50 (50% cytotoxic concentration). The selectivity index (SI) was calculated by the formula: SI = CC50 ÷ ED50.

b. Determination of In Vitro Antiviral Activity of Compounds Against Dengue, Banzi, and Yellow Fever (CPE Inhibition Assay)



[0122] Assay details are provided in the Sidwell and Huffman reference above.

Viruses:



[0123] Dengue virus type 2, New Guinea strain, was obtained from the Center for Disease Control. Two lines of African green monkey kidney cells were used to culture the virus (Vero) and to perform antiviral testing (MA-104). Both Yellow fever virus, 17D strain, prepared from infected mouse brain, and Banzi virus, H 336 strain, isolated from the serum of a febrile boy in South Africa, were obtained from ATCC. Vero cells were used with both of these viruses and for assay.

Cells and Media:



[0124] MA-104 cells (BioWhittaker, Inc., Walkersville, MD) and Vero cells (ATCC) were used in Medium 199 with 5% FBS and 0.1% NaHCO3 and without antibiotics.
Assay medium for dengue, yellow fever, and Banzi viruses was MEM, 2% FBS, 0.18% NaHCO3 and 50 µg gentamicin/mL.

[0125] Antiviral testing of the compounds of the present invention was performed according to the Sidwell and Huffman reference and similar to the above rhinovirus antiviral testing. Adequate cytopathic effect (CPE) readings were achieved after 5-6 days for each of these viruses.

c. Determination of In Vitro Antiviral Activity of Compounds Against West Nile Virus (CPE Inhibition Assay)



[0126] Assay details are provided in the Sidwell and Huffman reference cited above. West Nile virus. New York isolate derived from crow brain, was obtained from the Center for Disease Control. Vero cells were grown and used as described above. Test medium was MEM, 1% FBS, 0.1% NaHCO3 and 50 µg gentamicin/mL.

[0127] Antiviral testing of the compounds of the present invention was performed following the methods of Sidwell and Huffman which are similar to those used to assay for rhinovirus activity. Adequate cytopathic effect (CPE) readings were achieved after 5-6 days.

d. Determination of In Vitro Antiviral Activity of Compounds Against rhino, yellow fever, dengue, Banzi, and West Nile Viruses (Neutral Red Uptake Assay)



[0128] After performing the CPE inhibition assays above, an additional cytopathic detection method was used which is described in "Microtiter Assay for Interferon: Microspectrophotometric Quantitation of Cytopathic Effect," Appl. Environ. Microbiol. 31: 35-38 (1976). A Model EL309 microplate reader (Bio-Tek Instruments Inc.) was used to read the assay plate. ED50's and CD50's were calculated as above.

EXAMPLE OF A PHARMACEUTICAL FORMULATION



[0129] As a specific embodiment of an oral composition of a compound of the present invention, 50 mg of a compound of the present invention is formulated with sufficient finely divided lactose to provide a total amount of 580 to 590 mg to fill a size O hard gelatin capsule.


Claims

1. The use of a compound of structural formula I which is of the stereochemical configuration:

or a pharmaceutically acceptable salt thereof for the manufacture of a medicament for inhibiting HCV NS5B polymerase, inhibiting HCV viral replication or treating HCV infection; wherein B is

L is CH or N;

E is N or CR5;

W is O or S;

Y is H, C1-10 alkylcarbonyl, P3O9H4, P2O6H3, or P(O)R9R10;

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-4 alkyl, optionally substituted with 1 to 3 fluorine atoms,

C1-10 alkoxy, optionally substituted with C1-3 alkoxy or 1 to 3 fluorine atoms,

C2-6 alkenyloxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino;

R4 and R6 are each independently H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3;

R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen;

R12 and R13 are each independently hydrogen, methyl, hydroxymethyl, or fluoromethyl; and R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)C1-4 alkyl, OCH2O(C=O)OC1-4 alkyl, NHCHMeCO2Me, OCH(C1-4 alkyl)O(C=O)C1-4 alkyl,

wherein the compound is administered in the form of a tablet or capsule containing at least 0.1 percent by weight of the compound.


 
2. The use according to Claim 1 wherein the compound is of structural formula II of the indicated stereochemical configuration:

wherein B is

Y is H, C1-10 alkylcarbonyl, P3O9H4, or P(O)R9R10;

R1 is CF3, or C1-4 alkyl and one of R2 and R3 is OH or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-3 alkyl,

trifluoromethyl,

C1-4 alkoxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino;

R12 and R13 are each independently hydrogen or methyl; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)C1-4 alkyl, or OCH2O(C=O)C1-4 alkyl.


 
3. The use according to Claim 1 wherein B is


 
4. The use according to Claim 3 wherein the compound is of the structural formula III of the indicated stereochemical configuration:

wherein B is

Y is H, C1-10 alkylcarbonyl, P3O9H4, P2O6H3, or P(O)R9R10;

R1 is CF3, or C1-4 alkyl and one of R2 and R3 is OH or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

fluoro,

C1-3 alkyl,

trifluoromethyl,

C1-8 alkylcarbonyloxy,

C1-3 alkoxy, and

amino;

R6 is H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino,

C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3; and

R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen;

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)t-butyl, or OCH2O(C=O)iPr.


 
5. A compound of structural formula IV of the indicated stereochemical configuration:

or a pharmaceutically acceptable salt thereof;
wherein B is

L is CH or N;

E is N or CR5;

W is O or S;

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-4 alkyl, optionally substituted with 1 to 3 fluorine atoms,

C1-10 alkoxy, optionally substituted with C1-3 alkoxy or 1 to 3 fluorine atoms,

C2-6 alkenyloxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino;

R4 and R6 are each independently H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3;

R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen;

R12 and R13 are each independently hydrogen, methyl, hydroxymethyl, or fluoromethyl; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)C1-4 alkyl, OCH2O(C=O)OC1-4 alkyl, NHCHMeCO2Me, OCH(C1-4 alkyl)O(C=O)C1-4 alkyl,

provided that at least one of R9 and R10 is not hydroxy.


 
6. A compound according to Claim 5 of structural formula V of the indicated stereochemical configuration:

wherein B is

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-3 alkyl,

trifluoromethyl,

C1-4 alkoxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)C1-4 alkyl, or OCH2O(C=O)C1-4 alkyl, provided that at least one of R9 and R10 is not hydroxy.


 
7. A compound according to Claim 5 of structural formula VI:

wherein B is

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

fluoro,

C1-3 alkyl,

trifluoromethyl,

C1-3 alkoxy,

C1-8 alkylcarbonyloxy, and

amino; and

R9 and R10 are each independently hydroxy, OCH2CH2SC(=O)t-butyl, or OCH2O(C=O)iPr, provided that at least one of R9 and R10 is not hydroxy.


 
8. The use according to Claim 1 wherein the compound is of structural formula VII which is of the stereochemical configuration:

wherein B is

L is CH or N;

E is N or CR5;

W is O or S;

R1 is C2-4 alkenyl, C2-4 alkynyl, or C1-4 alkyl optionally substituted with amino, hydroxy, or 1 to 3 fluorine atoms and one of R2 and R3 is hydroxy or C1-4 alkoxy and the other of R2 and R3 is selected from the group consisting of

hydrogen,

hydroxy,

halogen,

C1-4 alkyl, optionally substituted with 1 to 3 fluorine atoms,

C1-10 alkoxy, optionally substituted with C1-3 alkoxy or 1 to 3 fluorine atoms,

C2-6 alkenyloxy,

C1-4 alkylthio,

C1-8 alkylcarbonyloxy,

aryloxycarbonyl,

azido,

amino,

C1-4 alkylamino, and

di(C1-4 alkyl)amino;

R4 and R6 are each independently H, OH, SH, NH2, C1-4 alkylamino, di(C1-4 alkyl)amino, C3-6 cycloalkylamino, halogen, C1-4 alkyl, C1-4 alkoxy, or CF3;

R5 is H, C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C1-4 alkylamino, CF3, or halogen; and

R12 and R13 are each independently hydrogen, methyl, hydroxymethyl, or fluoromethyl;

wherein the compound is administered in the form of a tablet or capsule containing at least 0.1 percent by weight of the compound.


 
9. A pharmaceutical composition comprising a compound according to any one of Claims 5 to 7 or a pharmaceutically acceptable salt thereof and a pharmaceutically acceptable carrier.
 
10. A compound according to any one of Claims 5 to 7 or a pharmaceutically acceptable salt thereof for use in a method of treatment of the human or animal body by therapy.
 
11. A combination of a compound according to any one of Claims 5 to 7 or a pharmaceutically acceptable salt thereof and a therapeutically effective amount of another agent active against HCV for simultaneous, separate or concurrent administration.
 
12. A combination according to Claim 11 wherein said agent active against HCV is ribavirin; levovirin; thymosin alpha-1; an inhibitor of NS3 serine protease; an inhibitor of inosine monophosphate dehydrogenase; interferon-a or pegylated interferon-a, alone or in combination with ribavirin or levovirin.
 
13. Use of a compound of any one of Claims 5 to 7 or a pharmaceutically acceptable salt thereof in the manufacture of a medicament for inhibiting HCV NS5B polymerase, inhibiting HCV viral replication or treating HCV infection.
 
14. The use of a compound according to any one of Claims 1 to 4, 8 or 13 or a pharmaceutically acceptable salt thereof in combination with a therapeutically effective amount of another agent active against HCV in the manufacture of a medicament for inhibiting HCV NS5B polymerase, inhibiting HCV viral replication or treating HCV infection, for simultaneous, separate or concurrent administration.
 
15. A use according to Claim 14 wherein said agent active against HCV is ribavirin; levovirin; thymosin alpha-1; an inhibitor of NS3 serine protease; an inhibitor of inosine monophosphate dehydrogenase; interferon-a or pegylated interferon-a, alone or in combination with ribavirin or levovirin.
 
16. A compound as defined in any one of Claims 5 to 7 or a pharmaceutically acceptable salt thereof for use in a method of inhibiting HCV NS5B polymerase, inhibiting HCV viral replication or treating HCV infection, optionally in combination with a therapeutically effective amount of another agent active against HCV as defined in Claim 14 or 15.
 
17. A compound as defined in any one of Claims 1 to 8 or a pharmaceutically acceptable salt thereof for use in a method of inhibiting HCV NS5B polymerase, inhibiting HCV viral replication or treating HCV infection, optionally in combination with a therapeutically effective amount of another agent active against HCV as defined in claim 14 or 15; wherein the compound is administered in the form of a tablet or capsule containing at least 0.1 percent by weight of the compound.
 


Ansprüche

1. Die Verwendung einer Verbindung der Strukturformel I, die die folgende stereochemische Konfiguration besitzt:

oder eines pharmazeutisch annehmbaren Salzes davon zur Herstellung eines Medikaments zur Inhibierung von HCV-NS5B-Polymerase, Inhibierung der HCV-Virusreplikation oder Behandlung einer HCV-Infektion, wobei B

L CH oder N ist,

E N oder CR5 ist,

W O oder S ist,

Y H, C1-10-Alkylcarbonyl, P3O9H4, P2O3H3 oder P(O)R9R10 ist,

R1 C2-4-Alkenyl, C2-4-Alkinyl oder C1-4-Alkyl ist, gegebenenfalls substituiert mit Amino, Hydroxy oder 1 bis 3 Fluoratomen, und eines von R2 und R3 Hydroxy oder C1-4-Alkoxy ist und das andere von R2 und R3 ausgewählt ist aus der Gruppe bestehend aus

Wasserstoff,

Hydroxy,

Halogen,

C1-4-Alkyl, gegebenenfalls substituiert mit 1 bis 3 Fluoratomen,

C1-10-Alkoxy, gegebenenfalls substituiert mit C1-3-Alkoxy oder 1 bis 3 Fluoratomen,

C2-6-Alkenyloxy,

C1-4-Alkylthio,

C1-8-Alkylcarbonyloxy,

Aryloxycarbonyl,

Azido,

Amino,

C1-4-Alkylamino und

Di(C1-4-alkyl)amino,

R4 und R6 jeweils unabhängig H, OH, SH, NH2, C1-4-Alkylamino, Di(C1-4-alkyl)amino, C3-6-Cycloalkylamino, Halogen, C1-4-Alkyl, C1-4-Alkoxy oder CF3 sind,

R5 H, C1-6-Alkyl, C2-6-Alkenyl, C2-6-Alkinyl, C1-4-Alkylamino, CF3 oder Halogen ist,

R12 und R13 jeweils unabhängig Wasserstoff, Methyl, Hydroxymethyl oder Fluormethyl sind, und R9 und R10 jeweils unabhängig Hydroxy, OCH2CH2SC(=O)C1-4-Alkyl, OCH2O(C=O)OC1-4-Alkyl, NHCHMeCO2Me, OCH(C1-4-Alkyl)O(C=O)C1-4-Alkyl,

sind, wobei die Verbindung in Form einer Tablette oder Kapsel verabreicht wird, die wenigstens 0,1 Gewichtsprozent der Verbindung enthält.


 
2. Die Verwendung gemäß Anspruch 1, wobei die Verbindung die Strukturformel II mit der angegeben stereochemischen Konfiguration besitzt:

wobei B

Y H, C1-10-Alkylcarbonyl, P3O9H4 oder P(O)R9R10 ist,

R1 CF3 oder C1-4-Alkyl ist, und eines von R2 und R3 OH oder C1-4-Alkoxy ist und das andere von R2 und R3 ausgewählt ist aus der Gruppe bestehend aus

Wasserstoff,

Hydroxy,

Halogen,

C1-3-Alkyl,

Trifluormethyl,

C1-4-Alkoxy,

C1-4-Alkylthio,

C1-8-Alkylcarbonyloxy,

Aryloxycarbonyl,

Azido,

Amino,

C1-4-Alkylamino und

Di(C1-4-alkyl)amino,

R12 und R13 jeweils unabhängig Wasserstoff oder Methyl sind, und

R9 und R10 jeweils unabhängig Hydroxy, OCH2CH2SC(=O)C1-4-Alkyl oder OCH2O(C=O)C1-4-Alkyl sind.


 
3. Die Verwendung gemäß Anspruch 1, wobei B


 
4. Die Verwendung gemäß Anspruch 3, wobei die Verbindung die Strukturformel III mit der angegebenen stereochemischen Konfiguration besitzt:

wobei B

Y H, C1-10-Alkylcarbonyl, P3O9H4, P2O6H3 oder P(O)R9R10 ist,

R1 CF3 oder C1-4-Alkyl ist, und eines von R2 und R3 OH oder C1-4-Alkoxy ist und das andere von R2 und R3 ausgewählt ist aus der Gruppe bestehend aus

Wasserstoff,

Hydroxy,

Fluor,

C1-3-Alkyl,

Trifluormethyl,

C1-8-Alkylcarbonyloxy,

C1-3-Alkoxy und

Amino,

R6 H, OH, SH, NH2, C1-4-Alkylamino, Di(C1-4-alkyl)amino, C3-6-Cycloalkylamino, Halogen, C1-4-Alkyl, C1-4-Alkoxy oder CF3 ist, und

R5 H, C1-6-Alkyl, C2-6-Alkenyl, C2-6-Alkinyl, C1-4-Alkylamino, CF3 oder Halogen ist,

R9 und R10 jeweils unabhängig Hydroxy, OCH2CH2SC(=O)t-Butyl oder OCH2O(C=O)iPr sind.


 
5. Eine Verbindung der Strukturformel IV mit der angegebenen stereochemischen Konfiguration:

oder ein pharmazeutisch annehmbares Salz davon,
wobei B

ist,

L CH oder N ist,

E N oder CR5 ist,

W O oder S ist,

R1 C2-4-Alkenyl, C2-4-Alkinyl oder C1-4-Alkyl, gegebenenfalls substituiert mit Amino, Hydroxy oder 1 bis 3 Fluoratomen, ist, und eines von R2 und R3 Hydroxy oder C1-4-Alkoxy ist und das andere von R2 und R3 ausgewählt ist aus der Gruppe bestehend aus

Wasserstoff,

Hydroxy,

Halogen,

C1-4-Alkyl, gegebenenfalls substituiert mit 1 bis 3 Fluoratomen,

C1-10-Alkoxy, gegebenenfalls substituiert mit C1-3-Alkoxy oder 1 bis 3 Fluoratomen,

C2-6-Alkenyloxy,

C1-4-Alkylthio,

C1-8-Alkylcarbonyloxy,

Aryloxycarbonyl,

Azido,

Amino,

C1-4-Alkylamino und

Di(C1-4-alkyl)amino,

R4 und R6 jeweils unabhängig H, OH, SH, NH2, C1-4-Alkylamino, Di(C1-4-alkyl)amino, C3-6-Cycloalkylamino, Halogen, C1-4-Alkyl, C1-4-Alkoxy oder CF3 sind,

R5 H, C1-6-Alkyl, C2-6-Alkenyl, C2-6-Alkinyl, C1-4-Alkylamino, CF3 oder Halogen ist,

R12 und R13 jeweils unabhängig Wasserstoff, Methyl, Hydroxymethyl oder Fluormethyl sind, und R9 und R10 jeweils unabhängig Hydroxy, OCH2CH2SC(=O)C1-4-Alkyl, OCH2O(C=O)OC1-4-Alkyl, NHCHMeCO2Me, OCH(C1-4-Alkyl)O(C=O)C1-4-alkyl,

sind,
mit der Maßgabe, dass wenigstens eines von R9 und R10 nicht Hydroxy ist.


 
6. Eine Verbindung gemäß Anspruch 5 der Strukturformel V mit der angegebenen stereochemischen Konfiguration:

wobei B

R1 C2-4-Alkenyl, C2-4-Alkinyl oder C1-4-Alkyl, gegebenenfalls substituiert mit Amino, Hydroxy oder 1 bis 3 Fluoratomen, ist, und eines von R2 und R3 Hydroxy oder C1-4-Alkoxy ist und das andere von R2 und R3 ausgewählt ist aus der Gruppe bestehend aus

Wasserstoff,

Hydroxy,

Halogen,

C1-3-Alkyl,

Trifluormethyl,

C1-4-Alkoxy,

C1-4-Alkylthio,

C1-8-Alkylcarbonyloxy,

Aryloxycarbonyl,

Azido,

Amino,

C1-4-Alkylamino und

Di(C1-4-alkyl)amino, und

R9 und R10 jeweils unabhängig Hydroxy, OCH2CH2SC(=O)C1-4-Alkyl oder OCH2O(C=O)C1-4-Alkyl sind, mit der Maßgabe, dass wenigstens eines von R9 und R10 nicht Hydroxy ist.


 
7. Eine Verbindung gemäß Anspruch 5 der Strukturformel VI:

wobei B

R1 C2-4-Alkenyl, C2-4-Alkinyl oder C1-4-Alkyl, gegebenenfalls substituiert mit Amino, Hydroxy oder 1 bis 3 Fluoratomen, ist, und eines von R2 und R3 Hydroxy oder C1-4-Alkoxy ist und das andere von R2 und R3 ausgewählt ist aus der Gruppe bestehend aus

Wasserstoff,

Hydroxy,

Fluor,

C1-3-Alkyl,

Trifluormethyl,

C1-3-Alkoxy,

C1-8-Alkylcarbonyloxy und

Amino, und

R9 und R10 jeweils unabhängig Hydroxy, OCH2CH2SC(=O)t-Butyl oder OCH2O(C=O)iPr sind, mit der Maßgabe, dass wenigstens eines von R9 und R10 nicht Hydroxy ist.


 
8. Die Verwendung gemäß Anspruch 1, wobei die Verbindung die Strukturformel VII besitzt, die die folgende stereochemische Konfiguration besitzt:

wobei B

L CH oder N ist,

E N oder CR5 ist,

W O oder S ist,

R1 C2-4-Alkenyl, C2-4-Alkinyl oder C1-4-Alkyl, gegebenenfalls substituiert mit Amino, Hydroxy oder 1 bis 3 Fluoratomen, ist, und eines von R2 und R3 Hydroxy oder C1-4-Alkoxy ist und das andere von R2 und R3 ausgewählt ist aus der Gruppe bestehend aus

Wasserstoff,

Hydroxy,

Halogen,

C1-4-Alkyl, gegebenenfalls substituiert mit 1 bis 3 Fluoratomen,

C1-10-Alkoxy, gegebenenfalls substituiert mit C1-3-Alkoxy oder 1 bis 3 Fluoratomen,

C2-6-Alkenyloxy,

C1-4-Alkylthio,

C1-8-Alkylcarbonyloxy,

Aryloxycarbonyl,

Azido,

Amino,

C1-4-Alkylamino und

Di(C1-4-alkyl)amino,

R4 und R6 jeweils unabhängig H, OH, SH, NH2, C1-4-Alkylamino, Di(C1-4-alkyl)amino, C3-6-Cycloalkylamino, Halogen, C1-4-Alkyl, C1-4-Alkoxy oder CF3 sind,

R5 H, C1-6-Alkyl, C2-6-Alkenyl, C2-6-Alkinyl, C1-4-Alkylamino, CF3 oder Halogen ist, und

R12 und R13 jeweils unabhängig Wasserstoff, Methyl, Hydroxymethyl oder Fluormethyl sind, wobei die Verbindung in Form einer Tablette oder Kapsel verabreicht wird, die wenigstens 0,1 Gew.-% der Verbindung enthält.


 
9. Eine pharmazeutische Zusammensetzung, die eine Verbindung gemäß einem der Ansprüche 5 bis 7 oder ein pharmazeutisch annehmbares Salz davon und einen pharmazeutisch annehmbaren Träger umfasst.
 
10. Eine Verbindung gemäß einem der Ansprüche 5 bis 7 oder ein pharmazeutisch annehmbares Salz davon zur Verwendung bei einem Verfahren zur Behandlung des Menschen- oder Tierkörpers durch Therapie.
 
11. Eine Kombination aus einer Verbindung gemäß einem der Ansprüche 5 bis 7 oder einem pharmazeutisch annehmbaren Salz davon und einer therapeutisch wirksamen Menge eines weiteren, gegen HCV wirksamen Mittels zur simultanen, separaten oder gleichzeitigen Verabreichung.
 
12. Eine Kombination gemäß Anspruch 11, wobei das gegen HCV wirksame Mittel Ribavirin, Levovirin, Thymosin-alpha-1, ein Inhibitor der NS3-Serin-Protease, ein Inhibitor der Inosinmonophosphat-Dehydrogenase, Interferon-a oder pegyliertes Interferon-a, alleine oder in Kombination mit Ribavirin oder Levovirin, ist.
 
13. Verwendung einer Verbindung nach einem der Ansprüche 5 bis 7 oder eines pharmazeutisch annehmbaren Salzes davon bei der Herstellung eines Medikaments zur Inhibierung von HCV-NS5B-Polymerase, Inhibierung der HCV-Virusreplikation oder Behandlung einer HCV-Infektion.
 
14. Die Verwendung einer Verbindung gemäß einem der Ansprüche 1 bis 4, 8 oder 13 oder eines pharmazeutisch annehmbaren Salzes davon in Kombination mit einer therapeutisch wirksame Menge eines weiteren Wirkstoffs gegen HCV bei der Herstellung eines Medikaments zur Inhibierung von HCV-NS5B-Polymerase, Inhibierung der HCV-Virusreplikation oder Behandlung einer HCV-Infektion zur simultanen, separaten oder gleichzeitigen Verabreichung.
 
15. Eine Verwendung gemäß Anspruch 14, wobei das gegen HCV wirksame Mittel Ribavirin, Levovirin, Thymosin-alpha-1, ein Inhibitor der NS3-Serin-Protease, ein Inhibitor der Inosinmonophosphat-Dehydrogenase, Interferon-a oder pegyliertes Interferon-a, alleine oder in Kombination mit Ribavirin oder Levovirin, ist.
 
16. Eine Verbindung wie in einem der Ansprüche 5 bis 7 definiert oder ein pharmazeutisch annehmbares Salz davon zur Verwendung bei einem Verfahren zur Inhibierung von HCV-NS5B-Polymerase, Inhibierung der HCV-Virusreplikation oder Behandlung einer HCV-Infektion, gegebenenfalls in Kombination mit einer therapeutisch wirksamen Menge eines weiteren Wirkstoffs gegen HCV wie in Anspruch 14 oder 15 definiert.
 
17. Eine Verbindung wie in einem der Ansprüche 1 bis 8 definiert oder ein pharmazeutisch annehmbares Salz davon zur Verwendung bei einem Verfahren zur Inhibierung von HCV-NS5B-Polymerase, Inhibierung der HCV-Virusreplikation oder Behandlung einer HCV-Infektion, gegebenenfalls in Kombination mit einer therapeutisch wirksamen Menge eines weiteren, gegen HCV wirksamen Mittels wie in Anspruch 14 oder 15 definiert, wobei die Verbindung in Form einer Tablette oder Kapsel verabreicht wird, die wenigstens 0,1 Gewichtsprozent der Verbindung enthält.
 


Revendications

1. Utilisation d'un composé de la formule structurale I qui est de la configuration stéréochimique:

ou d'un de ses sels pharmaceutiquement acceptables pour la fabrication d'un médicament pour l'inhibition de la polymérase NS5B du VHC, l'inhibition de la réplication virale du VHC ou le traitement d'une infection par VHC, dans lequel B est:

L est CH ou N;

E est N ou CR5;

W est O ou S;

Y est H, un C1-10 alkylcarbonyle, P3O9H4, P2O6H3 ou P(O)R9R10;

R1 est un C2-4 alcényle, un C2-4 alcynyle ou un C1-4 alkyle optionnellement substitué avec un amino, un hydroxy ou de 1 à 3 atomes de fluor et un de R2 et R3 est un hydroxy ou un C1-4 alcoxy et l'autre de R2 et R3 est choisi dans le groupe constitué de:

un hydrogène,

un hydroxy,

un halogène,

un C1-4 alkyle, optionnellement substitué avec de 1 à 3 atomes de fluor,

un C1-10 alcoxy, optionnellement substitué avec un C1-3 alcoxy ou de 1 à 3 atomes de fluor,

un C2-6 alcényloxy,

un C1-4 alkylthio,

un C1-8 alkylcarbonyloxy,

un aryloxycarbonyle,

un azido,

un amino,

un C1-4 alkylamino, et

un di(C1-4 alkyl)amino;

R4 et R6 sont chacun indépendamment H, OH, SH, NH2, un C1-4 alkylamino, un di(C1-4 alkyl)amino, un C3-6 cycloalkylamino, un halogène, un C1-4 alkyle, un C1-4 alcoxy ou CF3;

R5est H, un C1-6 alkyle, un C2-6 alcényle, un C2-6 alcynyle, un C1-4 alkylamino, CF3 ou un halogène;

R12 et R13 sont chacun indépendamment un hydrogène, un méthyle, un hydroxyméthyle ou un fluorométhyle; et

R9 et R10 sont chacun indépendamment un hydroxy, un OCH2CH2SC(=O)C1-4 alkyle, un OCH2O(C=O)OC1-4 alkyle, NHCHMeCO2Me, un OCH(C1-4 alkyl)O(C=O)C1-4 alkyle,

dans laquelle le composé est administré sous la forme d'un comprimé ou d'une capsule contenant au moins 0,1 pour-cent en poids du composé.


 
2. Utilisation selon la revendication 1, dans laquelle le composé est de la formule structurale II de la configuration stéréochimique indiquée:

dans lequel B est:

Y est H, un C1-10 alkylcarbonyle, P3O9H4 ou P(O)R9R10;

R1 est CF3 ou un C1-4 alkyle et un de R2 et R3 est OH ou un C1-4 alcoxy et l'autre de R2 et R3 est choisi dans le groupe constitué de:

un hydrogène,

un hydroxy,

un halogène,

un C1-3 alkyle,

un trifluorométhyle,

un C1-4 alcoxy,

un C1-4 alkylthio,

un C1-8 alkylcarbonyloxy,

un aryloxycarbonyle,

un azido,

un amino,

un C1-4 alkylamino, et

un di(C1-4 alkyl)amino;

R12 et R13 sont chacun indépendamment un hydrogène ou un méthyle; et

R9 et R10 sont chacun indépendamment un hydroxy, un OCH2CH2SC(=O)C1-4 alkyle ou un OCH2O(C=O)C1-4 alkyle.


 
3. Utilisation selon la revendication 1, dans lequel B est:


 
4. Utilisation selon la revendication 3, dans laquelle le composé est de la formule structurale III de la configuration stéréochimique indiquée:

dans lequel B est:

Y est H, un C1-10 alkylcarbonyle, P3O9H4, P2O6H3 ou P(O)R9R10,

R1 est CF3 ou un C1-4 alkyle et un de R2 et R3 est OH ou un C1-4 alcoxy et l'autre de R2 et R3 est choisi dans le groupe constitué de:

un hydrogène,

un hydroxy,

un fluoro,

un C1-3 alkyle,

un trifluorométhyle,

un C1-8 alkylcarbonyloxy,

un C1-3 alcoxy, et

un amino;

R6 est H, OH, SH, NH2, un C1-4 alkylamino, un di(C1-4 alkyl)amino, un C3-6 cycloalkylamino, un halogène, un C1-4 alkyle, un C1-4 alcoxy ou CF3; et

R5 est H, un C1-6 alkyle, un C2-6 alcényle, un C2-6 alcynyle, un C1-4 alkylamino, CF3 ou un halogène;

R9 et R10 sont chacun indépendamment un hydroxy, un OCH2CH2SC(=O)t-butyle ou un OCH2O(C=O)iPr.


 
5. Composé de la formule structurale IV de la configuration stéréochimique indiquée:

ou un de ses sels pharmaceutiquement acceptables;
dans lequel B est:

L est CH ou N;

E est N ou CR5;

W est O ou S;

R1 est un C2-4 alcényle, un C2-4 alcynyle ou un C1-4 alkyle optionnellement substitué avec un amino, un hydroxy ou de 1 à 3 atomes de fluor et un de R2 et R3 est un hydroxy ou un C1-4 alcoxy et l'autre de R2 et R3 est choisi dans le groupe constitué de:

un hydrogène,

un hydroxy,

un halogène,

un C1-4 alkyle, optionnellement substitué avec de 1 à 3 atomes de fluor,

un C1-10 alcoxy, optionnellement substitué avec un C1-3 alcoxy ou de 1 à 3 atomes de fluor,

un C2-6 alcényloxy,

un C1-4 alkylthio,

un C1-8 alkylcarbonyloxy,

un aryloxycarbonyle,

un azido,

un amino,

un C1-4 alkylamino, et

un di(C1-4 alkyl)amino;

R4 et R6 sont chacun indépendamment H, OH, SH, NH2, un C1-4 alkylamino, un di(C1-4 alkyl)amino, un C3-6 cycloalkylamino, un halogène, un C1-4 alkyle, un C1-4 alcoxy ou CF3;

R5est H, un C1-6 alkyle, un C2-6 alcényle, un C2-6 alcynyle, un C1-4 alkylamino, CF3 ou un halogène;

R12 et R13 sont chacun indépendamment un hydrogène, un méthyle, un hydroxyméthyle ou un fluorométhyle; et

R9 et R10 sont chacun indépendamment un hydroxy, un OCH2CH2SC(=O)C1-4 alkyle, un OCH2O(C=O)OC1-4 alkyle, NHCHMeCO2Me, un OCH(C1-4 alkyl)O(C=O)C1-4 alkyle,

à condition que au moins un de R9 et R10 ne soit pas un hydroxy.


 
6. Composé selon la revendication 5 de la formule structurale V de la configuration stéréochimique indiquée:

dans lequel B est:

R1 est un C2-4 alcényle, un C2-4 alcynyle ou un C1-4 alkyle optionnellement substitué avec un amino, un hydroxy ou de 1 à 3 atomes de fluor et un de R2 et R3 est un hydroxy ou un C1-4 alcoxy et l'autre de R2 et R3 est choisi dans le groupe constitué de:

un hydrogène,

un hydroxy,

un halogène,

un C1-3 alkyle,

un trifluorométhyle,

un C1-4 alcoxy,

un C1-4 alkylthio,

un C1-8 alkylcarbonyloxy,

un aryloxycarbonyle,

un azido,

un amino,

un C1-4 alkylamino, et

un di(C1-4 alkyl)amino; et

R9 et R10 sont chacun indépendamment un hydroxy, un OCH2CH2SC(=O)C1-4 alkyle ou un OCH2O(C=O)C1-4 alkyle, à condition que au moins un de R9 et R10 ne soit pas un hydroxy.


 
7. Composé selon la revendication 5 de la formule structurale VI:

dans lequel B est:

R1 est un C2-4 alcényle, un C2-4 alcynyle ou un C1-4 alkyle optionnellement substitué avec un amino, un hydroxy ou de 1 à 3 atomes de fluor et un de R2 et R3 est un hydroxy ou un C1-4 alcoxy et l'autre de R2 et R3 est choisi dans le groupe constitué de:

un hydrogène,

un hydroxy,

un fluoro,

un C1-3 alkyle,

un trifluorométhyle,

un C1-3 alcoxy,

un C1-8 alkylcarbonyloxy, et

un amino; et

R9 et R10 sont chacun indépendamment un hydroxy, un OCH2CH2SC(=O)t-butyle ou un OCH2O(C=O)iPr, à condition que au moins un de R9 et R10 ne soit pas un hydroxy.


 
8. Utilisation selon la revendication 1, dans laquelle le composé est de la formule structurale VII qui est de la configuration stéréochimique:

dans lequel B est:

L est CH ou N;

E est N ou CR5;

W est O ou S;

R1 est un C2-4 alcényle, un C2-4 alcynyle ou un C1-4 alkyle optionnellement substitué avec un amino, un hydroxy ou de 1 à 3 atomes de fluor et un de R2 et R3 est un hydroxy ou un C1-4 alcoxy et l'autre de R2 et R3 est choisi dans le groupe constitué de:

un hydrogène,

un hydroxy,

un halogène,

un C1-4 alkyle, optionnellement substitué avec de 1 à 3 atomes de fluor,

un C1-10 alcoxy, optionnellement substitué avec un C1-3 alcoxy ou de 1 à 3 atomes de fluor,

un C2-6 alcényloxy,

un C1-4 alkylthio,

un C1-8 alkylcarbonyloxy,

un aryloxycarbonyle,

un azido,

un amino,

un C1-4 alkylamino, et

un di(C1-4 alkyl)amino;

R4 et R6 sont chacun indépendamment H, OH, SH, NH2, un C1-4 alkylamino, un di(C1-4 alkyl)amino, un C3-6 cycloalkylamino, un halogène, un C1-4 alkyle, un C1-4 alcoxy ou CF3;

R5 est H, un C1-6 alkyle, un C2-6 alcényle, un C2-6 alcynyle, un C1-4 alkylamino, CF3 ou un halogène; et

R12 et R13 sont chacun indépendamment un hydrogène, un méthyle, un hydroxyméthyle ou un fluorométhyle;

dans laquelle le composé est administré sous la forme d'un comprimé ou d'une capsule contenant au moins 0,1 pour cent en poids du composé.


 
9. Composition pharmaceutique comprenant un composé selon l'une quelconque des revendications 5 à 7 ou un de ses sels pharmaceutiquement acceptables et un support pharmaceutiquement acceptable.
 
10. Composé selon l'une quelconque des revendications 5 à 7 ou un de ses sels pharmaceutiquement acceptables pour une utilisation dans une méthode de traitement du corps humain ou animal par une thérapie.
 
11. Combinaison d'un composé selon l'une quelconque des revendications 5 à 7 ou d'un de ses sels pharmaceutiquement acceptables et d'une quantité thérapeutiquement efficace d'un autre agent actif contre le VHC pour une administration simultanée, séparée ou combinée.
 
12. Combinaison selon la revendication 11, dans laquelle ledit agent actif contre le VHC est la ribavirine; la lévovirine; la thymosine alpha-1; un inhibiteur de la sérine protéase NS3; un inhibiteur de l'inosine monophosphate déshydrogénase; l'interféron-a ou un interféron-a pégylé, seul ou en combinaison avec la ribavirine ou la lévovirine.
 
13. Utilisation d'un composé selon l'une quelconque des revendications 5 à 7 ou d'un de ses sels pharmaceutiquement acceptables dans la fabrication d'un médicament pour l'inhibition de la polymérase NS5B du VHC, l'inhibition de la réplication virale du VHC ou le traitement d'une infection par VHC.
 
14. Utilisation d'un composé selon l'une quelconque des revendications 1 à 4, 8 ou 13 ou d'un de ses sels pharmaceutiquement acceptables en combinaison avec une quantité thérapeutiquement efficace d'un autre agent actif contre le VHC dans la fabrication d'un médicament pour l'inhibition de la polymérase NS5B du VHC, l'inhibition de la réplication virale du VHC ou le traitement d'une infection par VHC, pour une administration simultanée, séparée ou combinée.
 
15. Utilisation selon la revendication 14, dans laquelle ledit agent actif contre le VHC est la ribavirine; la lévovirine; la thymosine alpha-1; un inhibiteur de la sérine protéase NS3; un inhibiteur de l'inosine monophosphate déshydrogénase; l'interféron-a ou un interféron-a pégylé, seul ou en combinaison avec la ribavirine ou la lévovirine.
 
16. Composé selon l'une quelconque des revendications 5 à 7 ou un de ses sels pharmaceutiquement acceptables pour une utilisation dans une méthode d'inhibition de la polymérase NS5B du VHC, d'inhibition de la réplication virale du VHC ou de traitement d'une infection par VHC, optionnellement en combinaison avec une quantité thérapeutiquement efficace d'un autre agent actif contre le VHC selon la revendication 14 ou 15.
 
17. Composé selon l'une quelconque des revendications 1 à 8 ou un de ses sels pharmaceutiquement acceptables pour une utilisation dans une méthode d'inhibition de la polymérase NS5B du VHC, d'inhibition de la réplication virale du VHC ou de traitement d'une infection par VHC, optionnellement en combinaison avec une quantité thérapeutiquement efficace d'un autre agent actif contre le VHC selon la revendication 14 ou 15; où le composé est administré sous la forme d'un comprimé ou d'une capsule contenant au moins 0,1 pour cent en poids du composé.
 






Cited references

REFERENCES CITED IN THE DESCRIPTION



This list of references cited by the applicant is for the reader's convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard.

Patent documents cited in the description




Non-patent literature cited in the description