(19)
(11)EP 2 864 337 B1

(12)EUROPEAN PATENT SPECIFICATION

(45)Mention of the grant of the patent:
13.02.2019 Bulletin 2019/07

(21)Application number: 13733419.9

(22)Date of filing:  25.06.2013
(51)International Patent Classification (IPC): 
C07D 498/04(2006.01)
A61K 31/424(2006.01)
A61P 31/00(2006.01)
C07D 513/04(2006.01)
A61K 31/429(2006.01)
(86)International application number:
PCT/GB2013/051669
(87)International publication number:
WO 2014/001782 (03.01.2014 Gazette  2014/01)

(54)

ANTIMICROBIAL COMPOUNDS

ANTIMIKROBIELLE VERBINDUNGEN

COMPOSÉS ANTIMICROBIENS


(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30)Priority: 25.06.2012 GB 201211202

(43)Date of publication of application:
29.04.2015 Bulletin 2015/18

(73)Proprietor: Oxford University Innovation Limited
Botley Oxford OX2 0JB (GB)

(72)Inventors:
  • MOLONEY, Mark
    Dean Court, Oxford. OX2 9SD (GB)
  • JEONG, Yong-chu
    Suwon-si, Gyeonggi-do 443-733 (KR)

(74)Representative: HGF Limited 
8th Floor 140 London Wall
London EC2Y 5DN
London EC2Y 5DN (GB)


(56)References cited: : 
  
  • MARK D. ANDREWS ET AL: "Highly Functionalised Pyroglutamates by Intramolecular Aldol Reactions: Towards the Pyroglutamate Skeleton of Oxazolomycin", SYNLETT, vol. 1996, no. 07, 1996, pages 612-614, XP055073478, ISSN: 0936-5214, DOI: 10.1055/s-1996-5555
  • ANDREW G. BREWSTER ET AL: "Stereoselective intramolecular aldol reactions of (4R)-3-(3-oxobutanoyl)-1,3-thiazolidine-4- carboxylates believed to be directed by 'self-induced' axial chirality", CHEMICAL COMMUNICATIONS, no. 3, 1998, pages 299-300, XP055073435, ISSN: 1359-7345, DOI: 10.1039/a707388g
  • BREWSTER A G ET AL: "Memory of chirality effects in aldol cyclisations of 1-(3-oxobutyryl) derivatives of l-4-oxaproline and l-proline isopropyl esters", TETRAHEDRON LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 43, no. 21, 20 May 2002 (2002-05-20), pages 3919-3922, XP004352439, ISSN: 0040-4039, DOI: 10.1016/S0040-4039(02)00632-9
  • YONG-CHUL JEONG ET AL: "Control of chemoselectivity in Dieckmann ring closures leading to tetramic acids", ORGANIC & BIOMOLECULAR CHEMISTRY, vol. 9, no. 19, 20 June 2011 (2011-06-20), page 6663, XP055073494, ISSN: 1477-0520, DOI: 10.1039/c1ob05816a
  • CHLOE A. HOLLOWAY ET AL: "Novel Chiral Skeletons for Drug Discovery: Antibacterial Tetramic Acids", CHEMICAL BIOLOGY & DRUG DESIGN, vol. 78, no. 2, 16 August 2011 (2011-08-16), pages 229-235, XP055073438, ISSN: 1747-0277, DOI: 10.1111/j.1747-0285.2011.01133.x
  • YONG-CHUL JEONG ET AL: "Natural product inspired antibacterial tetramic acid libraries with dual enzyme inhibition", CHEMICAL SCIENCE, vol. 4, no. 3, 28 November 2012 (2012-11-28), page 1008, XP055073440, ISSN: 2041-6520, DOI: 10.1039/c2sc21713a
  
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).


Description


[0001] The present invention relates to certain tetramic acid derivatives and, in particular, bicyclic tetramic acid derivatives that are suitable for use in the preparation and development of antimicrobial (e.g. antibacterial or antifungal) compositions. The present invention also relates to the use of such compounds as antimicrobial (e.g. antibacterial or antifungal agents) and, in particular, as topical antibacterial or antifungal agents.

[0002] As the use of antibiotics becomes increasingly widespread, antibiotic-resistant strains of bacteria have evolved. As a result, there is a growing need for new antibiotics to combat the continuous emergence of such resistant strains.

[0003] Natural products containing a tetramate nucleus are known and some of these compounds are known to exhibit antibacterial activity. For example, streptolydigin and tirandamycin are known to have antibacterial activity against Gram-positive and Gram-negative bacteria, while reutericyclin is bacteriostatic or bactericidal to Gram-positive bacteria.

[0004] Attempts have been made to use such natural product as starting points to identify synthetic compound libraries of antibacterial agents. Attempts have also been made to use the tetramate nucleus as a scaffold for designing compounds with antibacterial activity. Examples of such compounds are described in WO 2008/014311 and EP 1116715. Tetramic acid derivatives are also described in the following documents:

MARK D. ANDREWS ET AL: "Highly Functionalised Pyroglutamates by Intramolecular Aldol Reactions: Towards the Pyroglutamate Skeleton of Oxazolomycin", SYNLETT, Vol. 1996, no. 07, 1 July 1996 (1996-07-01), pages 612-614,

ANDREW G, BREWSTER ET AL: "Stereoselective Intramolecular aldol reactions of (4R)-3-(3-oxobutanoyl)-1,3-thiazolidine-4carboxylates believed to be directed by 'self-induced' axial chirality",CHEMICAL COMMUNICATIONS, No. 3, 1998, pages 299-300,

BREWSTER A G ET AL: "Memory of Chirality effects in aldol cyclisations of 1-(3-oxobutyrul) derivatives of I-4-oxaproline and I-proline isopropyl esters", TETRAHEDRON LETTERS, ELSEVIER, AMSTERDAM, NL, vol. 43, no. 21, 20 May 2002 (2002-05-20), PAGES 3919-3922,

Yong-Chul Jeong ET AL: "Control of chemoselectivity in Dieckmann ring closures leading to tetramic acids",Organic & Biomolecular Chemistry, vol. 9, no. 19, 1 January 2011 (2011-01-01), page 6663, and

CHLOE A. HOLLOWAY ET AL: "Novel Chiral Skeletons for Drug Discovery: Antibacterial Tetramic Acides", CHEMICAL BIOLOGY & DRUG DESIGN, vol. 78, no. 2, 16 August 2011 (2011-08-16), pages 229-235.



[0005] It is among the objects of embodiments of the present invention to provide alternative compounds that can be used for the preparation and/or development of antibacterial compositions, and, in particular, antibacterial compositions for topical use.

SUMMARY OF THE INVENTION



[0006] In one aspect of the present invention, there is provided a compound as defined herein.

[0007] In another aspect, the present invention provides an antimicrobial composition comprising a compound as defined herein. Preferably, the composition is a topical composition.

[0008] In another aspect, the present invention provides the use of a compound as defined herein as an antimicrobial agent, such as a preservative.

[0009] In another aspect of the present invention, there is provided a compound as defined herein for use as a medicament for treating a topical microbial infection, such as a bacterial or fungal infection.

[0010] The present invention further provides a method of synthesising a compound as defined herein.

[0011] The present invention also provides a method of inhibiting bacterial RNA polymerase and/or undecaprenyl pyrophosphate synthase, which method comprising contacting a cell with an effective amount of a compound as defined herein.

[0012] In another aspect, the present invention provides a compound obtainable by, or obtained by, or directly obtained by a method of synthesis as defined herein.

[0013] Preferred, suitable, and optional features of any one particular aspect of the present invention are also preferred, suitable, and optional features of any other aspect.

DETAILED DESCRIPTION OF THE INVENTION


Definitions



[0014] Unless otherwise stated, the following terms used in the specification and claims have the following meanings set out below.

[0015] In this specification, the term "hydrocarbyl" refers to any substituent that consists of carbon and hydrogen atoms. The hydrocarbyl group may be saturated or unsaturated, or aromatic or aliphatic. The hydrocarbyl group may also be cyclic, straight chain or branched. Cyclic hydrocarbyl groups include monocyclic and polycyclic groups. Polycyclic groups include fused ring and bridged ring systems.

[0016] In this specification the term "alkyl" includes cyclic and non-cyclic, such as straight and branched chain alkyl groups.

[0017] The term "alkenyl" refers to hydrocarbyl groups containing at least one C=C bond. Alkenyl groups include cyclic, straight chain and branched alkenyl groups.

[0018] The term "aryl" refers to substituents comprising a monocyclic or polycyclic aromatic ring having at least 5 carbon atoms. The aryl group is an aryl hydrocarbyl group. Examples of aryl groups include, but are not limited to, phenyl, biphenyl, naphthyl and the like. In one embodiment, the "aryl" group may include a linking group attached the cyclic or polycyclic aromatic ring. The linking group may be of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. For example, the aryl group may be -C6H5 or -CH2C6H5. Thus, if a particular substituent, such as a hydrocarbyl group, is substituted with an aryl group, the substitution may take place via the linking group.

[0019] The term "halo" refers to fluoro, chloro, bromo and iodo.

[0020] The term "haloalkyl" is used herein to refer to an alkyl group in which one or more hydrogen atoms have been replaced by halogen atoms. The term "trihaloalkyl" refers to alkyl groups in which three hydrogen atoms have been replaced by halogen atoms, such as fluoroatoms. An example of a trihaloalkyl is trifluoroalkyl, -CF3.

[0021] The term "heterocyclyl", "heterocyclic" or "heterocycle" covers aromatic, non-aromatic, saturated or unsaturated monocyclic, fused, bridged, or spiro bicyclic heterocyclic ring system(s). Monocyclic heterocyclic rings contain from about 3 to 12 (suitably from 3 to 7) ring atoms, with from 1 to 5 (suitably 1, 2 or 3) heteroatoms selected from at least one of nitrogen, oxygen or sulphur in the ring. Bicyclic heterocycles contain from about 7 to about 17 ring atoms, suitably from 7 to 12 ring atoms. Bicyclic heterocyclic(s) rings may be fused, spiro, or bridged ring systems. Where the heterocycle comprises two or more ring structures, a heteroatom may be present in one or more of the rings. In one embodiment, the heterocycle group may include a linking group attached thereto. The linking group may be of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Thus, if a particular substituent, such as a hydrocarbyl or heterocyclic group, is substituted with another heterocyclic group, the substitution may occur via the linking group.

[0022] As the skilled person would appreciate, any heterocycle may be linked to another group via any suitable atom, such as via a carbon or nitrogen atom.

[0023] By "bridged ring systems" is meant ring systems in which two rings share more than two atoms, see for example Advanced Organic Chemistry, by Jerry March, 4th Edition, Wiley Interscience, pages 131-133, 1992.

[0024] As discussed above, the heterocyclic group may be an aromatic heterocyclic group (hereinafter a "heteroaryl" group). Examples of such heteroaryls include aromatic mono-, bi-, or polycyclic ring incorporating one or more (for example 1-4, particularly 1, 2 or 3) heteroatoms selected from nitrogen, oxygen or sulfur. Examples of heteroaryl groups are monocyclic and bicyclic groups containing from five to twelve ring members, and more usually from five to ten ring members. The heteroaryl group can be, for example, a 5- or 6-membered monocyclic ring or a 9- or 10-membered bicyclic ring, for example a bicyclic structure formed from fused five and six membered rings or two fused six membered rings.

[0025] The term "optionally substituted" refers to either groups, structures, or molecules that are substituted and those that are not substituted.

[0026] Where optional substituents are chosen from "one or more" groups, it is to be understood that this definition includes at least one of the substituents being chosen from one of the specified groups or two or more of the substituents being chosen from two or more of the specified groups. It is not necessary for all substituents to be chosen from one of the specified groups, although this may be preferred.

[0027] The phrase "compound of the invention" means those compounds which are disclosed herein, both generically and specifically. For the avoidance of doubt, the term "compound" covers the compound per se, as well as salts (including pharmaceutically acceptable salts) and solvates thereof.

[0028] It is to be appreciated that references to "treating" or "treatment" include prophylaxis as well as the alleviation of established symptoms of a condition, such as a bacterial or fungal infection. "Treating" or "treatment" of a state, disorder or condition therefore includes: (1) preventing or delaying the appearance of clinical symptoms of the state, disorder or condition developing in a human that may be afflicted with or predisposed to the state, disorder or condition but does not yet experience or display clinical or subclinical symptoms of the state, disorder or condition, (2) inhibiting the state, disorder or condition, i.e., arresting, reducing or delaying the development of the disease or a relapse thereof (in case of maintenance treatment) or at least one clinical or subclinical symptom thereof, or (3) relieving or attenuating the disease, i.e., causing regression of the state, disorder or condition or at least one of its clinical or subclinical symptoms.

[0029] A "therapeutically effective amount" means the amount of a compound that, when administered to a mammal for treating a disease, is sufficient to effect such treatment for the disease. The "therapeutically effective amount" will vary depending on the compound, the disease and its severity and the age, weight, etc., of the mammal to be treated.

Tetramic acid derivatives



[0030] The present invention provides a compound of the formula I or II or a pharmaceutically acceptable salt thereof for use as a medicament as defined in claims 1 to 3. The present invention also provides methods of synthesis as defined in claims 14 to 16. The present invention also provides a compound as defined in claims 17 to 24.

[0031] Preferably, the compound has the formula:





[0032] More preferably, the compound has the Formula Ia above.

[0033] In a preferred embodiment, Y is OH. Accordingly, it is preferred that Y is not a C1 to C10 alkyl.

[0034] The compounds of the present invention are useful in the development and/or manufacture of anti-microbial compositions, such as anti-bacterial or anti-fungal compositions. Without wishing to be bound by any theory, the antimicrobial activity is believed to be at least partially attributable to the [3.3.0] bicyclic structure and the substituent at the 7 position of the bicyclic ring structure. The antimicrobial activity may also depend on the nature of the substituents at at least one of the X, R2, R3, R1 and R12 positions. By varying at least one of these substituents, the antimicrobial activity of the compound may be optimised. Accordingly, the present invention provides the means for developing and optimising lead compounds for the synthesis of antimicrobial (e.g. antibacterial or antifungal) actives. In another embodiment, the present invention provides compounds for use as antimicrobial actives, and, in particular, antibacterial actives for topical use.

[0035] Preferably, X is O or S.

[0036] Preferably, Y is OH.

[0037] In one embodiment, R4 is H. In another embodiment, R1 is H or a C1 to C4 alkyl, such as methyl, ethyl, propyl (i-propyl or n-propyl) or butyl (n-butyl, t-butyl, s-butyl and i-butyl, preferably t-butyl). In another embodiment, both R1 and R4 are H. R2 may be H or a C1 to C6 alkyl, preferably a C1 to C3 alkyl. Where R2 is a C1 to C6 alkyl, methyl, ethyl and propyl (i-propyl or n-propyl) are preferred.

[0038] R3 may be H, C1 to C6 alkyl or a carbonyl-containing group. Where R3 is an alkyl group, the alkyl is preferably a C1 to C3 alkyl, more preferably methyl or ethyl. Where R3 is a carbonyl-containing group, the carbonyl-containing group may include an ester or ketone group. Suitable esters include -COORz, where Rz is an alkyl, preferably a C1 to C6 alkyl, more preferably methyl or ethyl. In one embodiment, Rz is a C1 to C6 alkyl that is substituted with an ether group, such as an OCH3 or OC2H5 group. For example, Rz may be -CH2CH2OCH3.

[0039] Where R3 is a ketone group, it may have the formula -C(O)Ry, where Ry is a C1 to C6 alkyl group. In one embodiment, R3 is selected from -C(O)C2H5 and -C(O)C4H9.

[0040] In one embodiment, R2 is CH3 and R3 is H, or R3 is CH3 and R2 is H. Alternatively, R2 is H or methyl and R3 is -COORz, where Rz is an alkyl, such as a methyl or ethyl group.

[0041] As mentioned above, R12 is selected from H, alkenyl, aryl, trihaloalkyl and C1 to C6 alkyl. In particular, the alkenyl and aryl groups may be substituted, for example, with one or more halo groups (such as F, Cl, Br and/or I), nitro groups, TBDMSO or ether groups (OCH3, OC2H5 or OPh). Preferably, at least one of R1 and R12 is H. Where R12 is an alkenyl group, the alkenyl group may include 1 to 8 carbon atoms, preferably 1 to 6 carbon atoms. The alkenyl group may include one or more double bonds. An example of a suitable alkenyl group is -C(CH3)2CH2CH=CH2.

[0042] Where R12 is a trihaloalkyl, the trihaloalkyl may include 1 to 3 carbon atoms, preferably 1 carbon atom. The halo group may be F, Cl, Br or I but F is preferred. An example of a suitable trihaloalkyl group is trifluoromethyl. Where R12 is a C1 to C6 alkyl group, the alkyl group is preferably a C1 to C4 alkyl group, such as methyl, ethyl, propyl (i-propyl or n-propyl) or butyl (n-butyl, t-butyl, s-butyl and i-butyl, preferably t-butyl). Where R12 is an aryl group, the aryl group may be phenyl. The aryl group may be substituted, for example, with one or more halo groups, such as F, Cl or Br. Other substituents include nitro substituents and TBDMSO. Examples of suitable R12 include:



where X is selected from F, Cl, Br, NO2 and CH2TBDMSO, and

where X1 and X2 are independently selected from F, Br and Cl. In one embodiment, X1 is F and X2 is Cl.

[0043] In one embodiment, R1 is t-butyl and R12 is H. In another embodiment, R1 is trifluoromethyl and R12 is H or methyl. In another embodiment, R12 is CH2=CHCH2C(CH3)2 and R1 is H. In yet another embodiment, R12 may be C6H3F2 and R1 may be H.

[0044] R5 and R7 are each a group of the formula L1-L2-R6 or L2-L1-R6, L1-L2-R6 being preferred. As noted above, L1 is a linker of the formula -[CR8R9]n-, where n is an integer of from 0 to 12, and R8 and R9 are each independently selected from H or C1 to C2 alkyl. Preferably, n is from 0 to 11, more preferably 0 to 6, even more preferably 0 to 4, for example, 0 or 1. Preferably, R8 and R9 are each independently selected from H or methyl. Where more than one -[CR8R9]- link is present (i.e. n is greater than 1), R8 may be the same or different in each instance of -[CR8R9]-. Similarly, R9 may be the same or different in each instance of -[CR8R9]-.

[0045] As noted above, L2 may be absent or a linker that is selected from O, S, SO, SO2, N(R'), C(O), C(O)O, OC(O), [O(CR'2)r]s, [(CR'2)rO]s CH(OR'), C(O)N(R'), N(R')C(O), N(R')C(O)N(R'), SO2N(R') or N(R')SO2 where R' and R" are each independently selected from hydrogen and a C1 to C2 alkyl, and where r is 1 or 2 and s is 1 to 4. In one embodiment, L2 is absent. In another embodiment, L2 is selected from O, C(O)O, OC(O), C(O)N(R') and N(R')C(O).

[0046] As mentioned above, R6 is selected from OR13, heterocyclic and C1 to C25 hydrocarbyl group. Where R6 is OR13, R13 is a C1 to C6 alkyl, preferably a C1 to C4 alkyl group, such as methyl, ethyl, propyl (i-propyl or n-propyl) or butyl (n-butyl, t-butyl, s-butyl and i-butyl, preferably t-butyl).

[0047] R6 may be a heterocyclic group. Suitable heterocyclic groups include aromatic and non-aromatic, saturated and unsaturated, and monocyclic and polycyclic heterocycles. Such groups may contain one or more heteroatoms, such as O, N and S. Where two or more heteroatoms are present, these may be the same or different. Where polycyclic heterocycles are used, the heteroatom may be present in one or more (e.g. all) of the cyclic groups. The heterocyclic group preferably has 5 to 12 ring members, for example, 5, 6, 7, 8 or 9 ring members. Suitable heterocyclic groups include saturated heterocyclic groups comprising 1 or 2 heteroatoms selected from O, N and S, preferably O and N. Examples of such saturated heterocycles include tetrahydrofuranyl, oxanyl, pyrrolidinyl, piperidinyl, azepanyl, morpholinyl, dioxanyl and piperazinyl. Suitable heterocyclic groups also include saturated heterocyclic groups comprising 1 or 2 heteroatoms selected from O, N and S. Examples of such unsaturated heterocycles include furanyl, pyrrol, imidazolyl, pyrazolyl, thiazolyl, isothiazolyl, diazinyl. The heterocycles may be fused to another ring, such as a cyclohexyl ring or benzene ring. Examples of such fused heterocycles include indole, isoindole and benzothiazole. The heterocyclic group may or may be coupled to the nitrogen of the enamine group, L1 or L2 via the heteroatom. Alternatively, the attachment may be via a C atom.

[0048] R6 may be a C1 to C25 hydrocarbyl group. This may be saturated or unsaturated, or aromatic or aliphatic. The hydrocarbyl group may be cyclic, straight chain or branched. In one embodiment, R6 is an alkyl group. Suitable alkyl groups include straight chain alkyl groups, such as those of the formula CiH2i+1, where i is 1 to 22, preferably 6 to 19. Cyclic alkyl groups, including monocyclic, polycyclic and bridged cycloalkyls, may also be used. Suitable monocyclic cycloalkyl groups may comprise C3 to C6 cycloalkyl groups, such as cyclopropane, cyclobutane, cyclopentyl and cyclohexyl. Such groups may also include hydrocarbyl linking groups, such as those of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0049] Suitable bridged cycloalkyls include adamantyl (e.g. 1-adamantyl), myrtanyl, norborane and 6,6-dimethylbicyclo[3.1.1]heptan-2-yl)methyl. Adamantyl is particularly preferred. This substituent may be substituted preferably with alkyl groups, such as methyl or ethyl groups. Where an adamantyl is used, it may be substituted with an alkyl group, such as a methyl group, at the 1 and/or 2 position. The hydrocarbyl substituents may also include hydrocarbyl linking groups, such as those of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0050] In another embodiment, the hydrocarbyl group may be or include an aryl group. Suitable aryl groups may have 6 to 12 carbon atoms. A preferred example is phenyl. The phenyl group may optionally be substituted, for example, with alkyl groups and/or halo groups, such as F, Cl and Br. These aryl groups may also include hydrocarbyl linking groups, such as those of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0051] In another embodiment, the hydrocarbyl may be or include an alkenyl group. Such groups may be derived from the alkyl groups defined in, for example, paragraphs [0046] and [0047] above by replacing at least one C-C bond with a C=C bond. A specific example is 1-cyclohexene. Such alkenyl groups may also include hydrocarbyl linking groups, such as those of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0052] As mentioned above, the heterocyclic group or hydrocarbyl group may be substituted with at least one functional group selected from alkenyl, alkyl, aryl, halo, trihaloalkyl, alcohol, keto, thioketo, sulfonyl, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea, =O, =S, sulfonyl and heterocyclic groups. These substituents, particularly, the aryl and heterocyclic groups may themselves be substituted further, for example, with alkenyl, alkyl, aryl, halo, trihaloalkyl, alcohol, keto, thioketo, sulfonyl, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea, =O, =S, sulfonyl and heterocyclic groups, such as those described herein. Where the heterocyclic group or hydrocarbyl group is substituted, at least one substituent must be selected from alkenyl, alkyl, aryl, halo, trihaloalkyl, alcohol, keto, S(O)R13, sulfonyl, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea, =O, =S, amine and heterocyclic groups. Where two or more substituents are present on the heterocyclic group or hydrocarbyl group, the second and one or more or all of the subsequent substituents may also be selected from alkenyl, alkyl, aryl, halo, trihaloalkyl, alcohol, keto, S(O)R13, sulfonyl, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea, =O, =S, amine and heterocyclic groups.

[0053] The heterocyclic group or hydrocarbyl group may be substituted with any alkenyl group. Suitable alkenyl groups are those having at least one C=C bond. The C=C may be coupled directly to the heterocyclic group or hydrocarbyl group or via a linking group of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3. The alkenyl group may have 2 to 20 carbon atoms, for example, 2 to 6 carbon atoms. The alkenyl group may be cyclic, straight chain or branched.

[0054] Where the substituent is an alkyl group, cyclic, straight chain and branched alkyl groups may be used. Suitable alkyl substituents may have 1 to 20 carbon atoms, for example, 1 to 6 carbon atoms. Preferred alkyl groups include methyl, ethyl, propyl (i-propyl or n-propyl) or butyl (n-butyl, t-butyl, s-butyl and i-butyl).

[0055] Where the substituent is an aryl group, the aryl group may be or include a hydrocarbyl aryl. Examples of aryl groups include, but are not limited to, phenyl, biphenyl, naphthyl and the like. Phenyl is preferred. In one embodiment, the aryl group may include a linking group attached the cyclic or polycyclic aromatic ring. The linking group may be of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3. For example, the aryl group may be -C6H5 or -CH2C6H5. The aryl group may itself be substituted, for example, with alkyl, aryl, halo, trihaloalkyl, alcohol, keto, thioketo, sulfonyl, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea, =O, =S, sulfonyl and heterocyclic groups as described herein. Examples of suitable substituents include alkyl groups, such as CH3, C2H5 and t-butyl groups. Other examples include OCH3, OC2H5 and OPh, phenyl, -OH, (CH2)gOH, where g is 1 to 3, OCH3, OC2H5, OPh, SCH3, SC2H5, SPh, NH2 N(CH3)2, F, Cl, Br, CF3, C(O)CH3 and S(O)CH3.

[0056] Where the substituent is halo, it may be a F, Cl, Br or I group. One or more halo substituents may be present. Where more than one halo substituent is present, the halo groups may be the same or different.

[0057] Where the substituent is a trihaloalkyl, the trihaloalkyl may be a trihalo(C1 - C6)alkyl. The halo substituent may be F, Cl, Br or I. The three halo substituents may be the same or different. Preferably, the trihaloalkyl is trihalomethyl, more preferably trifluoromethyl.

[0058] Where the substituent is an alcohol or a thioalcohol, the OH or SH substituent may be coupled directly to the heterocyclic or hydrocarbyl group. Alternatively, the OH substituent may be coupled to the heterocyclic or hydrocarbyl group via, for example, - [CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0059] Where the substituent is an ester, the substituent may have the formula C(O)OR16 or OC(O)R16, where R16 is a C1 to C6 alkyl (preferably methyl or ethyl), benzyl or phenyl group. Where the substituent is a thioester, the substituent may have the formula C(O)SR16 or SC(O)R16. The C(O)OR16, OC(O)R16 or C(O)SR16 or SC(O)R16 thioester may be coupled directly to the heterocyclic group or hydrocarbyl group or via a linking group, for example, of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0060] Where the substituent is an ether, the ether substituent may have or may include a group having the formula OR17, where R17 is C1 to C6 alkyl, phenyl or benzyl. Preferably, R17 is a methyl, ethyl, propyl (i-propyl or n-propyl) or butyl (n-butyl, t-butyl, s-butyl and i-butyl). Preferred ether substituents include OCH3, OC4H9, OCH(CH3)2 and OPh. The OR17 group may be coupled directly to the heterocyclic group or hydrocarbyl group or via a linking group, for example, of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0061] Where the substituent is a thioether, the thioether substituent may or may include a group having the formula SR17, where R17 is C1 to C6 alkyl, phenyl or benzyl. A preferred thioether substituent is SMe. The SR17 group may be coupled directly to the heterocyclic group or hydrocarbyl group or via a linking group e.g. of the formula-[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0062] Where the substituent is amide, the amide may have or may include a group of the formula N(R18)C(O)R19 or C(O)NR18R19. R18 may be selected from H and C1 to C10 alkyl, while R19 may be a C1 to C15 alkyl, trihaloalkyl (e.g. a trihalo(C1-C15)alkyl) or OR20, where R20 is a C1 to C6 alkyl. In one embodiment, R18 is H or C1 to C6 alkyl. Examples of suitable amide groups include HNC(O)C9H19, HNC(O)OC(CH3)3, HNC(O)C6F13, C(O)N(C6H13)(C6H13), HNC(O)Ph, N(CH3)C(O)C11H23, and HNC(O)1-adamantyl. The N(R18)C(O)R19 or C(O)NR18R19 group may be coupled directly to the heterocyclic group or hydrocarbyl group or via a linking group e.g. of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0063] Where the substituent is thioamide, the thioamide may have or may include a group having the formula N(R18)C(S)R19 or C(S)NR18R19. The N(R18)C(S)R19 or C(S)NR18R19 group may be coupled directly to the heterocyclic group or hydrocarbyl group or via a linking group e.g. of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0064] Where the substituent is urea, it may have the formula R30C(O)R31R32, where R30, R31 and R32 are independently selected from H and C1 to C6 alkyl, for example, methyl or ethyl.

[0065] Where the substituent is thiourea, it may have the formula R30C(S)R31R32, where R30, R31 and R32 are independently selected from H and C1 to C6 alkyl, for example, methyl or ethyl.

[0066] Where the substituent is a keto (or alkanoyl) group, it may have or may include a group of the formula C(O)R20, where R20 is a C1 to C6 alkyl, such as a methyl, ethyl, propyl (i-propyl or n-propyl) or butyl (n-butyl, t-butyl, s-butyl and i-butyl). An example of a suitable keto group is a C(O)CH3 group. In one embodiment, the keto group comprises an oxo substituted cycloalkyl group. The cycloalkyl group may have 4 to 8 carbon atoms, for example, 5 or 6 carbon atoms. The C(O)R20 group may be coupled directly to the hydrocarbyl group or heterocyclic group or may be coupled via a linker, for example, of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0067] Where the substituent is of the formula S(O)R13, R13 may be C1 to C6 alkyl, preferably methyl. The S(O)R13 may be coupled directly to the hydrocarbyl group or heterocyclic group or may be coupled via a linker, for example, of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0068] Where the substituent is a sulfonyl, it may include a substituent having the formula SO2R21, where R21 is a C1 to C6 alkyl or a trihalo(C1 to C6) alkyl, such as trifluoroalkyl. These groups may be coupled directly to the hydrocarbyl or heterocyclic group or via a linker, such as one of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0069] Where the substituent is an =O (oxo) or =S group, these are typically coupled to a ring atom of, for example, a heterocyclic, aryl or cyclic hydrocarbyl (e.g. alkyl or alkenyl) group. In one embodiment, an =O substituent is provided on a cyclopentyl ring.

[0070] Where the substituent is an amine, the amine may be or comprise a group of the formula NR22R23, where R22 and P23 are independently selected from H and C1 to C6 alkyl. In one embodiment, both R22 and R23 are H. In another embodiment, R22 and R23 are independently selected from H, methyl and ethyl. Examples of preferred amine substituents are NH2, N(CH3)2 and N(C2H5)2. The amines may optionally be provided in salt form, for example, as a salt of HCI. The NR22R23 group may be coupled directly to the heterocyclic or hydrocarbyl group or may be coupled via a linking group, such as one of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0071] Where the substituent is a heterocyclic group, it may be or may include aromatic and non-aromatic, saturated and unsaturated, and monocyclic and polycyclic heterocycles. Such groups may contain one or more heteroatoms, such as O, N and S. Where two or more heteroatoms are present, these may be the same or different. Where polycyclic heterocycles are used, the heteroatom may be present in one or more (e.g. all) of the cyclic groups. The heterocyclic group preferably has 5 to 12 ring members, for example, 5, 6, 7, 8 or 9 ring members. Suitable heterocyclic groups include saturated heterocyclic groups comprising 1 or 2 heteroatoms selected from O, N and S, preferably O and N. Examples of such saturated heterocycles include tetrahydrofuranyl, oxanyl, pyrrolidinyl, piperidinyl, azepanyl, morpholinyl, dioxanyl and piperazinyl. Suitable heterocyclic groups also include saturated heterocyclic groups comprising 1 or 2 heteroatoms selected from O, N and S. Examples of such unsaturated heterocycles include furanyl, pyrrol, imidazolyl, pyrazolyl, thiazolyl, isothiazolyl, diazinyl. The heterocycles may be fused to another ring, such as a cyclohexyl ring or benzene ring. Examples of such fused heterocycles include indole, isoindole and benzothiazole. The heterocyclic group may be coupled directly to the hydrocarbyl or heterocyclic group coupled to the enamine nitrogen. Alternatively, the heterocyclic group may be coupled indirectly via a linking group, such as one of the formula -[CR14R15]t-, where t is an integer of from 0 to 12, and R14 and R15 are each independently selected from H or C1 to C2 alkyl. Preferably, t is an integer from 0 to 6, for example, 1 to 3.

[0072] The heterocyclic group may itself be substituted. Suitable substituents are alkyl, aryl, alcohol, ether, thioether, amine, halo, trihaloalkyl, trihaloalkylether, keto, S(O)R25, where R25 is a C1 to C6 alkyl. These substituents are as defined herein. However, specific examples of such substituents include methyl, ethyl, phenyl, -OH, (CH2)9OH, where g is 1 to 3, OCH3, OC2H5, OPh, SCH3, SC2H5, SPh, NH2 N(CH3)2, F, Cl, Br, CF3, C(O)CH3 and S(O)CH3.

[0073] In a preferred embodiment, R6 is a phenyl group that is optionally substituted with at least one functional group selected from alkyl, aryl, halo, trihaloalkyl, alcohol, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea and heterocyclic group.

[0074] The phenyl group may be substituted with a group selected from cyclohexyl, C1 to C3 alkyl, halo, halo(C1 to C3) alkyl, OH, SH, heterocyclic and OR10 or SR10, where R10 is a C1 to C4 alkyl or phenyl group. Preferably, however, the phenyl group is substituted with a heterocyclic group selected from a piperidine and morpholine group, or where the phenyl group is fused to an aromatic heterocyclic ring, preferably a pyrrole ring.

[0075] In another preferred embodiment, the compound has the formula:

wherein R5 is selected from 1-adamantyl and CH2-C6H5, or

wherein R5 is CH2-C6H5.

[0076] In another preferred embodiment, the compound has the formula



[0077] In one embodiment, the following requirements are met:
X = O or S; R1 = t-Bu or CF3; R12 = H or Me; R2 = H, Me; R3 = H, Me, CO2Me, CO2Et, CO2(CH2)2OMe, COEt, COBu; R6 comprises adamantyl and substituted (e.g. with one or more methyl groups) adamantyl, cyclohexylmethyl, 6,6-dimethylbicyclo[3.1.1]heptan-2-yl)methyl, C6 to C13 alkyl, substituted aryl (e.g. 2-methyl-4-chlorophenyl, 2,4-dichlorophenyl, and 4-(trifluoromethyl)phenyl),

[0078] In an embodiment of the present invention, the compound has the Formula la and the following requirements are met:
X = O or S; R1 = t-Bu or CF3; R12 = H or Me; R2 = H or Me; R3 = H, Me; R6 comprises at least one of adamantyl and substituted (e.g. with one or more methyl groups) adamantyl, cyclohexylmethyl, 6,6-dimethylbicyclo[3.1.1]heptan-2-yl)methyl, C6 to C12 alkyl, substituted aryl (e.g. 2-methyl-4-chlorophenyl, 2,4-dichlorophenyl, and 4-(trifluoromethyl)phenyl) and/or (CH2)2OPh.

[0079] In another embodiment, the compound has the Formula II and the following requirements are met:
X = O or S; R1 = t-Bu; R12 = H; R2 = H or Me; R3 = H, Me, CO2Me, CO2Et, CO2(CH2)2OMe, COEt, COBu; R6 comprises at least one of adamantyl and substituted adamantyl (e.g. substituted with a C1 to C6 alkyl group, such as methyl), and C6 to C13 alkyl.

[0080] In yet another embodiment, the compound has the Formula I where Y is a C1 to C10 alkyl and the following requirements are met:

X = O; R1 = t-Bu; R11 = H; R2 = H; R3 = Me, CO2Me; R4 = H; R5 = (CH2)2OPh; Y = Me, or

X = O; R1 = t-Bu; R11 = H; R2 = H; R3 = Me, CO2Me; R4 = H; R5 = C4 alkyl or a C4 alkyl substituted with an ether group; Y = C9 alkyl.



[0081] Suitable or preferred features of any compounds of the present invention may also be suitable features of any other aspect.

[0082] A suitable pharmaceutically acceptable salt of a compound of the invention is, for example, a magnesium salt of the compound.

[0083] Compounds that have the same molecular formula but differ in the nature or sequence of bonding of their atoms or the arrangement of their atoms in space are termed "isomers". Isomers that differ in the arrangement of their atoms in space are termed "stereoisomers". Stereoisomers that are not mirror images of one another are termed "diastereomers" and those that are non-superimposable mirror images of each other are termed "enantiomers". When a compound has an asymmetric center, for example, it is bonded to four different groups, a pair of enantiomers is possible. An enantiomer can be characterized by the absolute configuration of its asymmetric center and is described by the R- and S-sequencing rules of Cahn and Prelog, or by the manner in which the molecule rotates the plane of polarized light and designated as dextrorotatory or levorotatory (i.e., as (+) or (-)-isomers respectively). A chiral compound can exist as either individual enantiomer or as a mixture thereof. A mixture containing equal proportions of the enantiomers is called a "racemic mixture".

[0084] The compounds of this invention may possess one or more asymmetric centers; such compounds can therefore be produced as individual (R)- or (S)-stereoisomers or as mixtures thereof. Unless indicated otherwise, the description or naming of a particular compound in the specification and claims is intended to include both individual enantiomers and mixtures, racemic or otherwise, thereof. The methods for the determination of stereochemistry and the separation of stereoisomers are well-known in the art (see discussion in Chapter 4 of "Advanced Organic Chemistry", 4th edition J. March, John Wiley and Sons, New York, 2001), for example by synthesis from optically active starting materials or by resolution of a racemic form. Some of the compounds of the invention may have geometric isomeric centres (E- and Z- isomers). It is to be understood that the present invention encompasses all optical, diastereoisomers and geometric isomers and mixtures thereof.

[0085] It is also to be understood that certain compounds of the invention may exist in solvated as well as unsolvated forms such as, for example, hydrated forms. It is to be understood that the invention encompasses all such solvated forms.

[0086] Compounds of the invention may exist in a number of different tautomeric forms and references to compounds of the invention include all such forms. For the avoidance of doubt, where a compound can exist in one of several tautomeric forms, and only one is specifically described or shown, all others are nevertheless embraced by compounds of the invention. Examples of tautomeric forms include keto-, enol-, and enolate-forms, as in, for example, the following tautomeric pairs: keto/enol (illustrated below).



[0087] It shall also be appreciated that compounds of the present invention may also be covalently linked (at any suitable position) to other groups such as, for example, solubilising moieties (for example, PEG polymers), moieties that enable them to be bound to a solid support (such as, for example, biotin-containing moieties), and targeting ligands (such as antibodies or antibody fragments).

[0088] The compounds of the present invention are useful as antimicrobial (e.g. antibacterial or antifungal agents). Accordingly, a further aspect of the present invention provides an antimicrobial composition, preferably a topical antibacterial or antifungal composition, comprising a compound as defined herein. As discussed above, the reference to "compound" covers all salts and solvates thereof.

[0089] The compounds of the present invention may be used as an antimicrobial compound in paper, fabric, building materials, packaging materials, coating and paint compositions, disinfectants, detergents, household products, cosmetics and suncreams.

[0090] The compounds of the present invention may be capable of inhibiting bacterial RNA polymerase and/or undecaprenyl pyrophosphate synthase. Accordingly, a further aspect of the invention provides a method of inhibiting bacterial RNA polymerase and/or undecaprenyl pyrophosphate synthase, which comprises contacting a cell with an effective amount of a compound as defined herein (or a pharmaceutically acceptable salt thereof). This contacting step may be performed ex-vivo or on the surface of a human or animal's skin.

[0091] The compounds of the present invention may be effective against Gram positive and/or Gram negative bacteria. For example, the compounds may be effective against Gram positive bacteria selected from at least one of Methicillin-resistant S. Aureus; Penicillin and erythromycin-resistant S. pneumonia; Vancomycin resistant E. Faecium; and Vancomycin susceptible E. Faecalis. Alternatively or additionally the compounds may be effective against Gram negative bacteria, such as H. influenzae. Some embodiments of the present invention are active against Gram positive bacteria but only weakly active against Gram negative bacteria. Preferred embodiments of the present invention are especially active against bacteria, such as MRSA (Methicillin resistant Staphylococcus aureus), MDR (multi-drug resistant bacteria), PRSP (Penicillin resistant Streptococcus pneumoniae) and VRE (Vancomycin resistant enterococcus).

[0092] In a preferred embodiment, the compounds of the present invention exhibit a minimum inhibitory concentration (MIC) of 16 µg/ml or less, preferably 12 µg/ml or less, more preferably 10 µg/ml or less, even more preferably 8 µg/ml or less, yet more preferably 6 µg/ml or less. In one embodiment, the MIC is 4 µg/ml or less, preferably less than 4 µg/ml or less, for example, 2 µg/ml or less. The compounds may exhibit the above MIC's against at least one bacteria selected from Gram positive and Gram negative bacteria. Examples of bacteria are discussed herein.

[0093] Procedures for determining MIC are well known in the art. For example, for bacteria, MICs may be determined based the Clinical and Laboratory Standards Institute (CLSI) methodology (Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard- seventh edition. 2006, M7-A7, CLSI, Wayne PA) by a 2-fold broth dilution technique in Mueller Hinton (pH7.4 Biorad). Overnight cultures may be diluted to obtain the final inoculum of 105 cfu/well. Incubation was 37°C overnight in ambient air. The MIC may be defined as the lowest concentration which inhibited all visual growth and expressed in µg/ml. For each bacterial species, all of the molecules were tested in the same experiment in order to give a head-to-head comparison.

[0094] For fungi, MICs may be determined for the antifungus by microdilution methods using RPMI 1640 medium buffered with morpholinopropanesulfonic acid (MOPS) and supplemented with L-glutamine as described by CLSI procedures (M27-A method) (Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved standard - third edition. 2006, M27-A2, CLSI, Wayne PA.). After incubation for 24-48 hours at 35°C, the lowest concentration of drug which produced 80% reduction in visible growth compared with control may be considered as the MIC.

[0095] Preferred examples of the compounds of the present invention are as follows:



































[0096] Preferably, the compound is selected from at least one of the following:





















[0097] More preferably, the compound is selected from at least one of the following:















[0098] Other examples include



















[0099] In one embodiment, the following compounds are excluded:

where X is O or S

where Y is H or CH3

Synthesis



[0100] In the description of the synthetic methods described below and in the referenced synthetic methods that are used to prepare the staring materials, it is to be understood that all proposed reaction conditions, including choice of solvent, reaction atmosphere, reaction temperature, duration of the experiment and workup procedures, can be selected by a person skilled in the art.

[0101] It is understood by one skilled in the art of organic synthesis that the functionality present on various portions of the molecule must be compatible with the reagents and reaction conditions utilised.

[0102] Necessary starting materials may be obtained by standard procedures of organic chemistry. The preparation of such starting materials is described in conjunction with the following representative process variants and within the accompanying Examples. Alternatively necessary starting materials are obtainable by analogous procedures to those illustrated which are within the ordinary skill of an organic chemist.

[0103] It will be appreciated that during the synthesis of the compounds of the invention in the processes defined below, or during the synthesis of certain starting materials, it may be desirable to protect certain substituent groups to prevent their undesired reaction. The skilled chemist will appreciate when such protection is required, and how such protecting groups may be put in place, and later removed.

[0104] For examples of protecting groups see one of the many general texts on the subject, for example, 'Protective Groups in Organic Synthesis' by Theodora Green (publisher: John Wiley & Sons). Protecting groups may be removed by any convenient method described in the literature or known to the skilled chemist as appropriate for the removal of the protecting group in question, such methods being chosen so as to effect removal of the protecting group with the minimum disturbance of groups elsewhere in the molecule.

[0105] In one aspect, the present invention provides method of synthesising a compound of the Formula II, which comprises:

reacting a compound of the formula III

with a carboxylic acid of the formula R7CO2H; or

reacting a compound of the formula III with i) a carboxylic acid of the formula R7CO2H or ii) an acid chloride of the formula R7COCl, to form a compound of the formula IV below, where R is a hydrocarbyl group,

and

reacting the compound of the Formula IV with a base (preferably 4-dimethylaminopyridine or triethylamine) to convert the O-acyl derivative to the corresponding C-acyl derivative.



[0106] In another aspect, the present invention also provides a method of synthesising a compound of the Formula I, wherein Y is a C1 to C10 alkyl, which comprises reacting a compound of Formula II with an amine of the formula R4R5NH.

[0107] In yet another aspect, the present invention provides a method of synthesising a compound of the Formula la, which comprises
  1. a) reacting a compound of the formula III

    with a compound of the formula R5NCO, or
    reacting a compound of the formula IV', where R is a hydrocarbyl group or R7:

    with an amine of the formula R4R5NH, with the proviso that, where compound III is reacted with R5NCO, R4 is H.


[0108] The compound of Formula IV may be formed by acylating a compound of Formula III, preferably with R7OC(O)Cl.

[0109] In one embodiment, the following reagents and/or reaction conditions are used in the synthesis of compounds of the invention:



[0110] Representative procedures for steps (e), (f) and (g) are given, by way of example, below.

[0111] Step (e): Acylation of tetramates. 3-Acyltetramic acids were prepared by reported method (Jeong, Y.-C. & Moloney, M. G. Synthesis of and tautomerism in 3-acyltetramic acids. J. Org. Chem. 76, 1342-1354 (2011)), and the direct acylation of tetramic acid with carboxylic acid was achieved by using 1.1 equivalent of N,N'-dicyclohexylcarbodiimide (DCC) and 1.2 equivalent of 4-(dimethylamino)pyridine (DMAP).

[0112] Step (f) for formation of 3-enamine tetramic acids. 3-Enamine tetramic acids were prepared from corresponding 3-acyltetramic acids and amine by using similar method with the synthesis of 3-carboxamide tetramic acids. (Ostrowska, K. et al. Monatshefte für Chemie 1999, 130, 555-562.)

[0113] Step (f) for formation of 3-Carboxamide tetramates. 3-Carboxamide tetramic acids were prepared by direct amine exchange of corresponding ester tetramic acid under refluxing conditions in toluene using the literature procedure (Folkes, A. et al. Bioorg. Med. Chem. Lett. 2002, 12, 1063-1066.), providing access to a range of amides in good to excellent yield (20-90 %).

[0114] Step (g) for formation of 3-carboxamide tetramates. 3-Carboxamide tetramic acids could be also prepared from tetramic acid with 1.1 equivalents of isocyanate in presence of 1.1 equivalents of DMAP.

[0115] The synthetic sequence for forming a compound of the Formula III is known in the art. Reference may be made to "Control of chemoselectivity in Dieckmann ring closures leading to tetramic acids", Y.-C. Jeong, M. Anwar, T. Minh Nguyen, B. Song Wei Tan, C. Li Lin Chai and M. G. Moloney, Org. Biomol. Chem., 2011, 9 (19), 6663 - 6669; "Synthesis of and tautomerism in 3-acyltetramic acids, Y.-C. Jeong, and M.G. Moloney, J. Org. Chem., 2011, 76, 1342-1354; "Enantioselective Synthesis of Tetramic Acids and Lactams from Threonine and their Antibiotic Activity", M. Anwar and M.G. Moloney, Tetrahedron:Asymmetry, 2010, 21, 1758-1770.; "Chemoselective Dieckmann Cyclisations Leading to Enantiopure Highly Functionalised Tetramic Acid Derivatives", M.D. Andrews, A.G. Brewster, J. Chuhan, K.M. Crapnell, A.J. Ibbett, M.G. Moloney, C.K. Prout, and D.J. Watkin, J. Chem. Soc., Perkin Trans 1, 1998, 223-235.). An example of a suitable synthetic sequence for forming a compound falling within Formula III is described below. In the sequence below, steps I and m have not been reported. These are described, by way of example, in paragraphs [00109] to [00112] below.


Step (I): Addition of Grignard reagents



[0116] Keto derivatives were obtained from the starting ester template by reaction with 5 equivalents of ethyllithium or of n-butyllithium. Due to the existence of the acidic proton at 3-position, excess amounts of the alkyllithium were required.

Step (m): Acylation with chloroformates



[0117] Diester derivatives were obtained by using 1.2 equivalents of butyl chloroformate and 2.2 equivalents of DMAP in CH2Cl2 with the starting tetramate under room temperature.

Example 1



[0118] The following compounds were tested:









[0119] MIC determination (bacteria); MICs were determined based on Clinical and Laboratory Standards Institute (CLSI) methodology (Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; Approved Standard- seventh edition. 2006, M7-A7, CLSI, Wayne PA) by a 2-fold broth dilution technique in Mueller Hinton (pH7.4 Biorad). For S. pneumoniae, the medium was supplemented with 2.5% laked horse blood. For H. influenzae, the medium was haemophilus test medium (H.T.M.). Overnight cultures were diluted to obtain the final inoculum of 105 cfu/well. Incubation was 37°C overnight in ambient air. The MIC was defined as the lowest concentration which inhibited all visual growth and expressed in µg/ml. For each bacterial species, all of the molecules were tested in the same experiment in order to give a head-to-head comparison. The results are shown in Table 2 below:

[0120] MIC determination (fungus); MICs were determined for the antifungus by microdilution methods using RPMI 1640 medium buffered with morpholinopropanesulfonic acid (MOPS) and supplemented with L-glutamine as described by CLSI procedures (M27-A method) (Reference method for broth dilution antifungal susceptibility testing of yeasts; Approved standard - third edition. 2006, M27-A2, CLSI, Wayne PA.). After incubation for 24-48 hours at 35°C, the lowest concentration of drug which produced 80% reduction in visible growth compared with control was considered as the MIC. The results are shown in Table 2 below.

[0121] The pharmacological properties of these derivatives were also determined and the results are shown in Table 1 below. These properties can be determined according to techniques that are well known in the art.
Table 1. Pharmacological properties of selected tetramic acidsa,c
 RNAUPPDEPRBCCANHEKPMBSOLPPB
 IC50, µMIC50, µMIC50, µMIC50, µMMIC, µg/mlLD50, µMLD50, µMµM%
(±)-2A 17 0.38 10.8 >100 >32 30.3 30.3 75-150 -c
(±)-2E 31 0.68 84.3 >100 >32 90.9 90.9 >300 96.6
6C 4.0 3.2 >100 >100 >32 90.9 >90.9 >300 99.8
6G 2.4 1.9 >100 >100 >32 10.1 >90.9 >300 -c
a; abbreviation; RNA; In vitro activity against E. Coli RNAP, UPP; In vitro activity against S. pneumonia UPPS, DEP; In vitro activity in depolarization of S. aureus membrane, RBC; In vitro mammalian_red blood cell membrane lysis activity, CAN; In vitro antifungal activity against Candida albicans, HEK; in vitro toxicities against human embryonic kidney 293 cells, PMB; in vitro toxicities human peripheral blood cells, SOL; aqueous solubility at pH 7.4 (water with 2% DMSO), PPB; % ratio of plasma protein binding. c; Not determined.
Table 2. In vitro antibiotic activity (MIC, µg/mL) of tetramic acidsa,
 S1S26S4S2E1E2P1P9P9BH3H4
(±)-2A 1 2 1 1 1 1 1 1 1 2 1
(±)-2E 4 4 4 2 2 1 2 1 1 4 0.12
6C 2 2 2 1 2 1 0.5 0.5 1 64 0.25
6G 0.5 1 1 0.5 1 0.5 2 1 4 >64 2
                       
a; abbreviation; S1; S. aureus 1, ATCC13709 in vivo (methicillin sensitive), S26; S. aureus 26, ATCC25923 (vancomycin susceptible), S4; S. aureus 4, Oxford, S2; S. aureus 2, (MRSA in vivo), E1; E. faecalis 1, ATCC29212 VanS (vancomycin susceptible), E2; E. faecium 1, VanA (vancomycin resistant), P1; S. pneumonia 1, ATCC49619 (erythromycin susceptible), P9; S. pneumonia 9, (multi-drug resistant), P9B; S. pneumonia 9 in presence of 2.5 % horse blood, H3; H. influenzae 3, ATCC31517 MMSA, H4; H. influenzae 4, LS2 Efflux ko, b; All analogues are inactive (MIC > 64 µg/ml) against E. coli 1, ATCC25922 (non pathogenic strain), E. coli 50, Ec49 No Efflux and P. aeruginosa 1, ATCC27853.

Example 2



[0122] Various compounds illustrative of the compounds of the present invention were synthesised and tested for their bioactivity. Microbiological assays were performed by the hole- plate method with the test organism Staphylococcus aureus N.C.T.C. 6571 or E. coli X580. Solutions (100 µl) of the compounds to be tested (4mg/ml) were loaded into wells in bioassay plates, and incubated overnight at 37°C. The diameters of the resultant inhibition zones were measured.

[0123] The results are shown in the tables below.
CompoundBioactivity (mm)Log PPolar Surface Area (PSA)Molecular Surface Area (MSA)% PSA/MS A% Yield
S. aureus (4 mg/ml)E. coli (4 mg/ml)


16 x 1.73 112.66 431.52 26.11 80


x 16 2.68 72.91 500.64 14.56 97


18 X 2.54 66.84 414.75 16.12 84
CompoundBioactivity (mm)Log PPolar Surface Area (PSA)Molecular Surface Area (MSA)% PSA/MSA% Yield
S. aureusE. coli
0.01 mg/ml0.001 mg/ml0.01 mg/ml0.001 mg/ml


22 X 15 15 1.23 69.64 545.16 12.8 38 (from crude)


27 21 14 X 1.05 69.64 552.66 12.6 56


25 19 14 X 0.72 115.46 584.98 19.7 35 (from crude)


25 21 14 X 1.37 69.64 567.97 12.3 60
CompoundBioactivity (mm)Log PPolar Surface Area (PSA)Molecular Surface Area(MSA)% PSA/MSA% Yield
E. coliS. aureus
(4 mg/ml)(4 mg/ml)


XX 2.0 Cm 2.56 66.84 410.79 16.27 78


XX 1.7 Cm 2.56 66.84 410.86 16.27 82


XX 2.3 Cm 3.79 76.07 668.64 11.38 72
CompoundBioactivity (mm)Log PPolar Surface Area (PSA)Molecular Surface Area (MSA)% PSA/MSA% Yield
E. coliS. aureus
0.5 mg/ml0.25 mg /ml0.5 mg/ml0.25 mg/ ml


X X 13 1247 2.39 78.87 821.82 9.59 28 (from crude)


X X 26 25 4.38 69.64 503.62 13.82 41 (from crude)



Claims

1. A compound of the formula I or II or a pharmaceutically acceptable salt thereof for use as a medicament as an antimicrobial agent:



wherein:

X is O, S or SO2,

Y is OH or C1 to C10 alkyl,

R1, R2 and R4 are each independently selected from H and C1 to C6 alkyl,

R3 is selected from a functional group selected from H, C1 to C6 alkyl, and a carbonyl-containing group,

R12 is: a substituent selected from H, alkenyl, "aryl", trihaloalkyl and C1 to C6 alkyl, wherein the "aryl" group is a monocyclic or polycyclic aromatic hydrocarbyl group having at least 5 carbon atoms, or comprises a monocyclic or polycyclic aromatic hydrocarbyl group having at least 5 carbon atoms and a linking group of the formula -[CR14R15]t that is coupled to the cyclic or polycyclic aromatic ring, wherein R14 and R15 are each independently selected from H or C1 or C2 alkyl and t is an integer of 0 to 12, and wherein substitution takes place via the linking group; and

wherein, where R12 is alkenyl or "aryl", the alkenyl or "aryl" group is optionally substituted with one or more of halo, nitro, ether and t-butyldimethylsilylether (TBDMSO).

R5 and R7 are each a group of the formula L1-L2-R6 or L2-L1-R6,

where L1 is a linker of the formula -[CR8R9]n-, where n is an integer of from 0 to 12, and R8 and R9 are each independently selected from H or C1 to C2 alkyl, and

where L2 is absent or a linker that is selected from O, S, SO, SO2, N(R'), C(O), C(O)O, OC(O), [O(CR'2)r]s, [(CR'2)rO]s CH(OR'), C(O)N(R'), N(R')C(O), N(R')C(O)N(R'), SO2N(R') or N(R')SO2 where R' and R" are each independently selected from hydrogen and a C1 to C2 alkyl, and where r is 1 or 2 and s is 1 to 4, and

where R6 is selected from OR13, heterocyclic and C1 to C25 hydrocarbyl group, wherein R13 is a C1 to C6 alkyl, and said heterocyclic and hydrocarbyl group is optionally substituted with at least one functional group selected from alkenyl, alkyl, aryl, halo, trihaloalkyl, alcohol, keto, S(O)R13, sulfonyl, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea, =O, =S, amine and heterocyclic group, and

wherein alkyl is defined as cyclic and non-cyclic alkyl.


 
2. A compound for use as claimed in claim 1, which has the formula:

or


 
3. A compound for use as claimed in any one of the preceding claims, wherein n is 0 or 1 and L2 is absent.
 
4. A compound for use as claimed in any one of the preceding claims, wherein R6 is a C6 to C19 alkyl.
 
5. A compound for use as claimed in claim 4, where in the alkyl is a bridged ring system that is optionally selected from adamantyl and myrtanyl.
 
6. A compound for use as claimed in any one of claims 1 to 3, wherein R6 is a phenyl group that is optionally substituted with at least one functional group selected from alkyl, aryl, halo, trihaloalkyl, alcohol, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea and heterocyclic group.
 
7. A compound for use as claimed in claim 6, wherein the phenyl group is substituted with a heterocyclic group selected from a piperidine and morpholine group, or where the phenyl group is fused to an aromatic heterocyclic ring, preferably a pyrrole ring.
 
8. A compound for use as claimed in any one of the preceding claims, wherein R3 is selected from H, methyl, C(O)R11, and C(O)O[CR8R9]nOR11, where R11 is a C1 to C4 alkyl group.
 
9. A compound for use as claimed in any one of the preceding claims, having the formula:

wherein R5 is selected from 1-adamantyl and CH2-C6H5, or

wherein R5 is CH2-C6H5; or


 
10. A compound for use as claimed in claim 1, wherein R12 is aryl selected from

a)

b)

where X is selected from F, Cl, Br, NO2 and CH2OTBDMS, and

c)

where X1 and X2 are independently selected from F, Br and Cl.


 
11. A compound for use as claimed in claim 1, wherein R12 is selected from H, alkenyl, aryl, trihaloalkyl and C1 to C6 alkyl.
 
12. A compound for use as claimed in any one of the preceding claims, which is a medicament for treating or preventing a topical microbial infection.
 
13. A compound for use as claimed any one of the preceding claims, for use as a medicament as an antibacterial or antifungal agent.
 
14. A method of synthesising a compound of the Formula II, which comprises

a) reacting a compound of the formula III

with a carboxylic acid of the formula R7CO2H; or
reacting a compound of the formula III with i) a carboxylic acid of the formula R7CO2H or ii) an acid chloride of the formula R7COCl,
to form a compound of the formula IV below,

and
reacting the compound of the Formula IV with a base to convert the O-acyl derivative to the corresponding C-acyl derivative.


 
15. A method of synthesising a compound of the Formula I, wherein Y is a C1 to C10 alkyl, which comprises reacting a compound of Formula II with an amine of the formula R4R5NH.
 
16. A method of synthesising a compound of the Formula la, which comprises

a) reacting a compound of the formula III

with a compound of the formula R5NCO, or
reacting a compound of the formula IV, with an amine of the formula R4R5NH, with the proviso that, where compound III is reacted with R5NCO, R4 is H.


 
17. A compound of the formula I or II:





wherein:

X is O, S or SO2,

Y is OH or C1 to C10 alkyl,

R1, R2 and R4 are each independently selected from H and C1 to C6 alkyl,

R3 is selected from a functional group selected from H, C1 to C6 alkyl, and a carbonyl-containing group,

R12 is a substituent selected from H, alkenyl, "aryl", trihaloalkyl and C1 to C6 alkyl, wherein the "aryl" group is a monocyclic or polycyclic aromatic hydrocarbyl group having at least 5 carbon atoms, or comprises a monocyclic or polycyclic aromatic hydrocarbyl group having at least 5 carbon atoms and a linking group of the formula -[CR14R15]t that is coupled to the cyclic or polycyclic aromatic ring, wherein R14 and R15 are each independently selected from H or C1 or C2 alkyl and t is an integer of 0 to 12, and wherein substitution takes place via the linking group; and

wherein, where R12 is alkenyl or "aryl", the alkenyl or "aryl" group is optionally substituted with one or more of halo, nitro, ether and t-butyldimethylsilylether (TBDMSO),

R5 and R7 are each a group of the formula L1-L2-R6 or L2-L1-R6,

where L1 is a linker of the formula -[CR8R9]n-, where n is an integer of from 0 to 12, and R8 and R9 are each independently selected from H or C1 to C2 alkyl, and

where L2 is absent or a linker that is selected from O, S, SO, SO2, N(R'), C(O), C(O)O, OC(O), [O(CR'2)r]s, [(CR'2)rO]s CH(OR'), C(O)N(R'), N(R')C(O), N(R')C(O)N(R'), SO2N(R') or N(R')SO2 where R' and R" are each independently selected from hydrogen and a C1 to C2 alkyl, and where r is 1 or 2 and s is 1 to 4, and

where R6 is a C6 to C19 alkyl;

R6 is a phenyl group that is optionally substituted with at least one functional group selected from alkyl, aryl, halo, trihaloalkyl, alcohol, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea and heterocyclic group;

R6 is a OR13, wherein R13 is a C1 to C6 alkyl, or

R6 is a heterocyclic group that is optionally substituted with at least one functional group selected from alkenyl, alkyl, aryl and halo;
and

wherein alkyl is defined as cyclic and non-cyclic alkyl.


 
18. A compound as claimed in claim 17, where R6 is a C6 to C19 alkyl; or R6 is a phenyl group that is optionally substituted with at least one functional group selected from alkyl, aryl, halo, trihaloalkyl, alcohol, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea and heterocyclic group.
 
19. A compound as claimed in claim 17, where R6 is an alkyl that is a bridged ring system that is optionally selected from adamantyl and myrtanyl.
 
20. A compound as claimed in claim 17, wherein R6 is a phenyl group that is substituted with a heterocyclic group selected from a piperidine and morpholine group, or where the phenyl group is fused to an aromatic heterocyclic ring, preferably a pyrrole ring.
 
21. A compound as claimed in claim 17, wherein R12 selected from H, alkenyl, aryl, trihaloalkyl and C1 to C6 alkyl.
 
22. A compound as claimed in claim 17 having the formula:

wherein R5 is selected from 1-adamantyl and CH2-C6H5, or

wherein R5 is CH2-C6H5; or


 
23. A compound of the formula I or a pharmaceutically acceptable salt thereof:

wherein:

X is O, S or SO2,

Y is OH or C1 to C10 alkyl,

R1, R2 and R4 are each independently selected from H and C1 to C6 alkyl,

R3 is selected from a functional group selected from H, C1 to C6 alkyl, and a carbonyl-containing group,

R12 is a substituent selected from H, alkenyl, "aryl", trihaloalkyl and C1 to C6 alkyl, wherein the "aryl" group is a monocyclic or polycyclic aromatic hydrocarbyl group having at least 5 carbon atoms, or comprises a monocyclic or polycyclic aromatic hydrocarbyl group having at least 5 carbon atoms and a linking group of the formula -[CR14R15]t that is coupled to the cyclic or polycyclic aromatic ring, wherein R14 and R15 are each independently selected from H or C1 or C2 alkyl and t is an integer of 0 to 12, and wherein substitution takes place via the linking group; and

wherein, where R12 is alkenyl or "aryl", the alkenyl or "aryl" group is optionally substituted with one or more of halo, nitro, ether and t-butyldimethylsilylether (TBDMSO),

R5 is a group of the formula L1-L2-R6 or L2-L1-R6,

where L1 is a linker of the formula -[CR8R9]n-, where n is an integer of from 0 to 12, and R8 and R9 are each independently selected from H or C1 to C2 alkyl, and

where L2 is absent or a linker that is selected from O, S, SO, SO2, N(R'), C(O), C(O)O, OC(O), [O(CR'2)r]s, [(CR'2)rO]s CH(OR'), C(O)N(R'), N(R')C(O), N(R')C(O)N(R'), SO2N(R') or N(R')SO2 where R' and R" are each independently selected from hydrogen and a C1 to C2 alkyl, and where r is 1 or 2 and s is 1 to 4, and

where R6 is selected from OR13, heterocyclic and C1 to C25 hydrocarbyl group, wherein R13 is a C1 to C6 alkyl, and said heterocyclic and hydrocarbyl group is optionally substituted with at least one functional group selected from alkenyl, alkyl, aryl, halo, trihaloalkyl, alcohol, keto, S(O)R13, sulfonyl, thio-alcohol, ester, thioester, ether, thioether, amide, thioamide, urea, thiourea, =O, =S, amine and heterocyclic group, and

wherein alkyl is defined as cyclic and non-cyclic alkyl.


 
24. A compound as claimed in claim 23, wherein R12 is selected from H, alkenyl, aryl, trihaloalkyl and C1 to C6 alkyl.
 


Ansprüche

1. Verbindung der Formel I oder II oder ein pharmazeutisch annehmbares Salz davon zur Verwendung als ein Medikament als ein antimikrobielles Mittel:



wobei:

X O, S oder SO2 ist,

Y OH oder C1- bis C10-Alkyl ist,

R1, R2 und R4 jeweils unabhängig ausgewählt sind aus H und C1- bis C6-Alkyl,

R3 ausgewählt ist aus einer Funktionsgruppe ausgewählt aus H, C1- bis C6-Alkyl, und einer Carbonyl-enthaltenden Gruppe,

R12 Folgendes ist: ein Substituent ausgewählt aus H, Alkenyl, "Aryl", Trihaloalkyl und C1- bis C6-Alkyl, wobei die "Aryl"-Gruppe eine monocyclische oder polycyclische aromatische Hydrocarbyl-Gruppe mit mindestens 5 Kohlenstoffatomen ist, oder eine monocyclische oder polycyclische aromatische Hydrocarbyl-Gruppe mit mindestens 5 Kohlenstoffatomen und eine Verbindungsgruppe der Formel -[CR14R15]t umfasst, die mit dem cyclischen oder polycyclischen aromatischen Ring gekoppelt ist, wobei R14 und R15 jeweils unabhängig ausgewählt sind aus H oder C1- oder C2-Alkyl, und t eine Ganzzahl von 0 bis 12 ist, und wobei eine Substitution über die Verbindungsgruppe erfolgt; und

wobei, wo R12 Alkenyl oder "Aryl" ist, die Alkenyl- oder "Aryl"-Gruppe optional mit einem oder mehreren von Halo, Nitro, Ether und t-Butyldimethylsilylether (TBDMSO) substituiert ist.

R5 und R7 jeweils einer Gruppe der Formel L1-L2-R6 oder L2-L1-R6 sind,

wo L1 ein Linker der Formel -[CR8R9]n- ist, wo n eine Ganzzahl von 0 bis 12 ist, und R9 und R9 jeweils unabhängig ausgewählt sind aus H oder C1- bis C2-Alkyl, und

wo L2 fehlt oder ein Linker ist, der ausgewählt ist aus O, S, SO, SO2, N(R'), C(O), C(O)O, OC(O), [O(CR'2)r]s, [(CR'2)rO]s CH(OR'), C(O)N(R'), N(R')C(O), N(R')C(O)N(R'), SO2N(R') oder N(R')SO2, wo R' und R" jeweils unabhängig ausgewählt sind aus Wasserstoff und einem C1- bis C2-Alkyl, und wo r 1 oder 2 ist und s 1 bis 4 ist, und

wo R6 ausgewählt ist aus OR13, heterocyclischer und C1- bis C25-Hydrocarbyl-Gruppe, wobei R13 ein C1- bis C6-Alkyl ist, und die heterocyclische und Hydrocarbyl-Gruppe optional substituiert ist mit mindestens einer Funktionsgruppe ausgewählt aus Alkenyl, Alkyl, Aryl, Halo, Trihaloalkyl, Alkohol, Keto, S(O)R13, Sulfonyl, Thio-Alkohol, Ester, Thioester, Ether, Thioether, Amid, Thioamid, Urea, Thiourea, =O, =S, Amin und heterocyclischer Gruppe, und

wobei Alkyl als cyclisches und nicht-cyclisches Alkyl definiert ist.


 
2. Verbindung zur Verwendung nach Anspruch 1, die die folgende Formel aufweist:

oder


 
3. Verbindung zur Verwendung nach einem der vorherigen Ansprüche, wobei n 0 oder 1 ist und L2 fehlt.
 
4. Verbindung zur Verwendung nach einem der vorherigen Ansprüche, wobei R6 ein C6- bis C19-Alkyl ist.
 
5. Verbindung zur Verwendung nach Anspruch 4, wo in dem Alkyl ein überbrücktes Ringsystem ist, das optional ausgewählt ist aus Adamantyl und Myrtanyl.
 
6. Verbindung zur Verwendung nach einem der Ansprüche 1 bis 3, wobei R6 eine Phenyl-Gruppe ist, die optional substituiert ist mit mindestens einer Funktionsgruppe ausgewählt aus Alkyl, Aryl, Halo, Trihaloalkyl, Alkohol, Thio-Alkohol, Ester, Thioester, Ether, Thioether, Amid, Thioamid, Urea, Thiourea und einer heterocyclischen Gruppe.
 
7. Verbindung zur Verwendung nach Anspruch 6, wobei die Phenyl-Gruppe mit einer heterocyclischen Gruppe substituiert ist, ausgewählt aus einer Piperidin- und Morpholin-Gruppe, oder wo die Phenyl-Gruppe zu einem aromatischen heterocyclischen Ring kondensiert ist, vorzugsweise einem Pyrrol-Ring.
 
8. Verbindung zur Verwendung nach einem der vorherigen Ansprüche, wobei R3 ausgewählt ist aus H, Methyl, C(O)R11, und C(O)O[CR8R9]nOR11, wo R11 eine C1- bis C4-Alkyl-Gruppe ist.
 
9. Verbindung zur Verwendung nach einem der vorherigen Ansprüche mit der folgenden Formel:

wobei R5 ausgewählt ist aus 1-Adamantyl und CH2-C6H5, oder

wobei R5 CH2-C6H5 ist, oder


 
10. Verbindung zur Verwendung nach Anspruch 1, wobei R12 Aryl ist, ausgewählt aus

a)

b)

c)

wo X ausgewählt ist aus F, Cl, Br, NO2 und CH2OTBDMS, und wo X1 und X2 unabhängig ausgewählt sind aus F, Br und Cl.


 
11. Verbindung zur Verwendung nach Anspruch 1, wobei R12 ausgewählt ist aus H, Alkenyl, Aryl, Trihaloalkyl und C1- bis C6-Alkyl.
 
12. Verbindung zur Verwendung nach einem der vorherigen Ansprüche, die ein Medikament zur Behandlung oder Prävention einer topischen mikrobiellen Infektion ist.
 
13. Verbindung zur Verwendung nach einem der vorherigen Ansprüche, zur Verwendung als Medikament als ein antibakterielles oder antimykotisches Mittel.
 
14. Verfahren zum Synthetisieren einer Verbindung der Formel II, das Folgendes umfasst

a) Reagieren einer Verbindung der Formel III

mit einer Carbonsäure der Formel R7CO2H; oder
Reagieren einer Verbindung der Formel III mit i) einer Carbonsäure der Formel R7CO2H oder ii) einem Säurechlorid der Formel R7COCI,
um eine Verbindung der Formel IV unten zu bilden,

und
Reagieren der Verbindung der Formel IV mit einer Base, um das O-Acyl-Derivat zu dem entsprechenden C-Acyl-Derivat umzuwandeln.


 
15. Verfahren zum Synthetisieren einer Verbindung der Formel I, wobei Y ein C1- bis C10-Alkyl ist, das ein Reagieren einer Verbindung der Formel II mit einem Amin der Formel R4R5NH umfasst.
 
16. Verfahren zum Synthetisieren einer Verbindung der Formel Ia, das Folgendes umfasst

a) Reagieren einer Verbindung der Formel III

mit einer Verbindung der Formel R5NCO, oder
Reagieren einer Verbindung der Formel IV mit einem Amin der Formel R4R5NH, mit der Maßgabe, dass wo Verbindung III mit R5NCO reagiert wird, R4 H ist.


 
17. Verbindung der Formel I oder II:



wobei:

X O, S oder SO2 ist,

Y OH oder C1- bis C10-Alkyl ist,

R1, R2 und R4 jeweils unabhängig ausgewählt sind aus H und C1- bis C6-Alkyl,

R3 ausgewählt ist aus einer Funktionsgruppe ausgewählt aus H, C1- bis C6-Alkyl, und einer Carbonyl-enthaltenden Gruppe,

R12 ein Substituent ist, ausgewählt aus H, Alkenyl, "Aryl", Trihaloalkyl und C1- bis C6-Alkyl, wobei die "Aryl"-Gruppe eine monocyclische oder polycyclische aromatische Hydrocarbyl-Gruppe mit mindestens 5 Kohlenstoffatomen ist, oder eine monocyclische oder polycyclische aromatische Hydrocarbyl-Gruppe mit mindestens 5 Kohlenstoffatomen und eine Verbindungsgruppe der Formel -[CR14R15]t umfasst, die mit dem cyclischen oder polycyclischen aromatischen Ring gekoppelt ist, wobei R14 und R15 jeweils unabhängig ausgewählt sind aus H oder C1- oder C2-Alkyl, und t eine Ganzzahl von 0 bis 12 ist, und wobei eine Substitution über die Verbindungsgruppe erfolgt; und

wobei, wo R12 Alkenyl oder "Aryl" ist, die Alkenyl- oder "Aryl"-Gruppe optional mit einem oder mehreren von Halo, Nitro, Ether und t-Butyldimethylsilylether (TBDMSO) substituiert ist,

R5 und R7 jeweils einer Gruppe der Formel L1-L2-R6 oder L2-L1-R6 sind,

wo L1 ein Linker der Formel -[CR8R9]n- ist, wo n eine Ganzzahl von 0 bis 12 ist, und R8 und R9 jeweils unabhängig ausgewählt sind aus H oder C1- bis C2-Alkyl, und

wo L2 fehlt oder ein Linker ist, der ausgewählt ist aus O, S, SO, SO2, N(R'), C(O), C(O)O, OC(O), [O(CR'2)r]s, [(CR'2)rO]s CH(OR'), C(O)N(R'), N(R')C(O), N(R')C(O)N(R'), SO2N(R') oder N(R')SO2, wo R' und R" jeweils unabhängig ausgewählt sind aus Wasserstoff und einem C1- bis C2-Alkyl, und wo r 1 oder 2 ist und s 1 bis 4 ist, und

wo R6 ein C6- bis C19-Alkyl ist;

R6 eine Phenyl-Gruppe ist, die optional substituiert ist mit mindestens einer Funktionsgruppe ausgewählt aus Alkyl, Aryl, Halo, Trihaloalkyl, Alkohol, Thio-Alkohol, Ester, Thioester, Ether, Thioether, Amid, Thioamid, Urea, Thiourea und einer heterocyclischen Gruppe.

R6 ein OR13 ist, wobei R13 ein C1- bis C6-Alkyl ist, oder

R6 eine heterocyclische Gruppe ist, die optional substituiert ist mit mindestens einer Funktionsgruppe ausgewählt aus Alkenyl, Alkyl, Aryl und Halo; und

wobei Alkyl als cyclisches und nicht-cyclisches Alkyl definiert ist.


 
18. Verbindung nach Anspruch 17, wo R6 ein C6- bis C19-Alkyl ist; oder R6 eine Phenyl-Gruppe ist, die optional substituiert ist mit mindestens einer Funktionsgruppe ausgewählt aus Alkyl, Aryl, Halo, Trihaloalkyl, Alkohol, Thio-Alkohol, Ester, Thioester, Ether, Thioether, Amid, Thioamid, Urea, Thiourea und einer heterocyclischen Gruppe.
 
19. Verbindung nach Anspruch 17, wo R6 ein Alkyl ist, das ein überbrücktes Ringsystem ist, das optional ausgewählt ist aus Adamantyl und Myrtanyl.
 
20. Verbindung nach Anspruch 17, wobei R6 eine Phenyl-Gruppe ist, die mit einer heterocyclischen Gruppe substituiert ist, ausgewählt aus einer Piperidin und Morpholin Gruppe, oder wo die Phenyl-Gruppe zu einem aromatischen heterocyclischen Ring kondensiert ist, vorzugsweise einem Pyrrol-Ring.
 
21. Verbindung nach Anspruch 17, wobei R12 ausgewählt aus H, Alkenyl, Aryl, Trihaloalkyl und C1- bis C6-Alkyl.
 
22. Verbindung nach Anspruch 17 mit der folgenden Formel:

wobei R5 ausgewählt ist aus 1-Adamantyl und CH2-C6H5, oder

wobei R5 CH2-C6H5 ist, oder


 
23. Verbindung der Formel I oder ein pharmazeutisch annehmbares Salz davon:

wobei:

X O, S oder SO2 ist,

Y OH oder C1- bis C10-Alkyl ist,

R1, R2 und R4 jeweils unabhängig ausgewählt sind aus H und C1- bis C6-Alkyl,

R3 ausgewählt ist aus einer Funktionsgruppe ausgewählt aus H, C1- bis C6-Alkyl, und einer Carbonyl-enthaltenden Gruppe,

R12 ein Substituent ist, ausgewählt aus H, Alkenyl, "Aryl", Trihaloalkyl und C1- bis C6-Alkyl, wobei die "Aryl"-Gruppe eine monocyclische oder polycyclische aromatische Hydrocarbyl-Gruppe mit mindestens 5 Kohlenstoffatomen ist, oder eine monocyclische oder polycyclische aromatische Hydrocarbyl-Gruppe mit mindestens 5 Kohlenstoffatomen und eine Verbindungsgruppe der Formel -[CR14R15]t umfasst, die mit dem cyclischen oder polycyclischen aromatischen Ring gekoppelt ist, wobei R14 und R15 jeweils unabhängig ausgewählt sind aus H oder C1- oder C2-Alkyl, und t eine Ganzzahl von 0 bis 12 ist, und wobei eine Substitution über die Verbindungsgruppe erfolgt; und

wobei, wo R12 Alkenyl oder "Aryl" ist, die Alkenyl- oder "Aryl"-Gruppe optional mit einem oder mehreren von Halo, Nitro, Ether und t-Butyldimethylsilylether (TBDMSO) substituiert ist,

R5 eine Gruppe der Formel L1-L2-R6 oder L2-L1-R6 ist,

wo L1 ein Linker der Formel -[CR8R9]n- ist, wo n eine Ganzzahl von 0 bis 12 ist, und R8 und R9 jeweils unabhängig ausgewählt sind aus H oder C1- bis C2-Alkyl, und

wo L2 fehlt oder ein Linker ist, der ausgewählt ist aus O, S, SO, SO2, N(R'), C(O), C(O)O, OC(O), [O(CR'2)r]s, [(CR'2)rO]s CH(OR'), C(O)N(R'), N(R')C(O), N(R')C(O)N(R'), SO2N(R') oder N(R')SO2, wo R' und R" jeweils unabhängig ausgewählt sind aus Wasserstoff und einem C1- bis C2-Alkyl, und wo r 1 oder 2 ist und s 1 bis 4 ist, und

wo R6 ausgewählt ist aus OR13, heterocyclischer und C1- bis C25-Hydrocarbyl-Gruppe, wobei R13 ein C1- bis C6-Alkyl ist, und die heterocyclische und Hydrocarbyl-Gruppe optional substituiert ist mit mindestens einer Funktionsgruppe ausgewählt aus Alkenyl, Alkyl, Aryl, Halo, Trihaloalkyl, Alkohol, Keto, S(O)R13, Sulfonyl, Thio-Alkohol, Ester, Thioester, Ether, Thioether, Amid, Thioamid, Urea, Thiourea, =O, =S, Amin und heterocyclischer Gruppe, und

wobei Alkyl als cyclisches und nicht-cyclisches Alkyl definiert ist.


 
24. Verbindung nach Anspruch 23, wobei R12 ausgewählt ist aus H, Alkenyl, Aryl, Trihaloalkyl und C1- bis C6-Alkyl.
 


Revendications

1. Composé de la formule I ou II ou un sel phamarceutiquement acceptable de celui-ci destiné à être utilisé comme un médicament comme un agent antimicrobien :



dans lesquelles :

X représente un atome O, S ou un groupe SO2,

Y représente un groupe OH ou alkyle de C1 à C10,

R1, R2 et R4 sont chacun indépendamment choisis parmi un atome H et un groupe alkyle de C1 à C6,

R3 est choisi parmi un groupe fonctionnel choisi parmi un atome H, un groupe alkyle de C1 à C6 et un groupe contenant un carbonyle,

R12 est : un substituant choisi parmi un atome H, une groupe alcényle, « aryle », trihaloalkyle et alkyle de C1 à C6, dans lequel le groupe « aryle » est un groupe hydrocarbyle aromatique monocyclique ou polycyclique avec au moins 5 atomes de carbone, ou comprend un groupe hydrocarbyle aromatique monocyclique ou polycyclique avec au moins 5 atomes de carbone et un groupe de liaison de la formule -[CR14R15]t qui est couplé au noyau aromatique cyclique ou polycyclique, dans lequel R14 et R15 sont chacun indépendamment choisis parmi un atome H ou un groupe alkyle de C1 ou C2 et t est un nombre entier de 0 à 12, et dans lequel prend place une substitution par l'intermédiaire du groupe de liaison ; et

dans lequel R12 est un groupe alcényle ou « aryle », le groupe alcényle ou « aryle » est éventuellement substitué par au moins un halo, un nitro, un éther et un t-butyldiméthylsilyléther (TBDMSO),

R5 et R7 sont chacun un groupe de la formule L1-L2-R6 ou L2-L1-R6,

dans lesquelles L1 est un lieur de la formule -[CR8R9]n-, dans laquelle n est un nombre entier de 0 à 12, et R8 et R9 sont chacun indépendamment choisis parmi un atome H ou un groupe alkyle de C1 à C2, et

dans lesquelles L2 est absent ou un lieur qui est choisi parmi un atome O, S, un groupe SO, SO2, N(R'), C(O), C(O)O, OC(O), [O(CR'2)r]s, [(CR'2)rO]s CH(OR'), C(O)N(R'), N(R')C(O), N(R')C(O)N(R'), SO2N(R') ou N(R')SO2, dans lesquels R' et R" sont chacun indépendamment choisis parmi un atome hydrogène et un groupe alkyle de C1 à C2, et dans lesquels r vaut 1 ou 2 et s vaut de 1 à 4, et dans lesquelles R6 est choisi parmi un groupe OR13, un groupe hétérocyclique et hydrocarbyle de C1 à C25, dans lequel R13 est un groupe alkyle de C1 à C6, et ledit groupe hétérocyclique et hydrocarbyle est éventuellement substitué par au moins un groupe fonctionnel choisi parmi un groupe alcényle, alkyle, aryle, halo, trihaloalkyle, alcool, céto, S(O)R13, sulfonyle, thio-alcool, ester, thioester, éther, thioéther, amide, thioamide, urée, thio-urée =O, =S, amine et un groupe hétérocyclique, et

dans lesquelles le groupe alkyle est défini comme un groupe alkyle cyclique et non cyclique.


 
2. Composé destiné à être utilisé selon la revendication 1, lequel a la formule :

ou


 
3. Composé destiné à être utilisé selon l'une quelconque des revendications précédentes, dans lequel n vaut 0 ou 1 et L2 est absent.
 
4. Composé destiné à être utilisé selon l'une quelconque des revendications précédentes, dans lequel R6 est un groupe alkyle de C6 à C19.
 
5. Composé destiné à être utilisé selon la revendication 4, dans lequel, dans le groupe alkyle, se trouve un système de noyau ponté qui est éventuellement choisi parmi le groupe adamantyle et myrtanyle.
 
6. Composé destiné à être utilisé selon l'une quelconque des revendications 1 à 3, dans lequel R6 est un groupe phényle qui est éventuellement substitué par au moins un groupe fonctionnel choisi parmi un groupe alkyle, aryle, halo, trihaloalkyle, alcool, thio-alcool, ester, thioester, éther, thioéther, amide, thioamide, urée, thio-urée et un groupe hétérocyclique.
 
7. Composé destiné à être utilisé selon la revendication 6, dans lequel le groupe phényle est substitué par un groupe hétérocyclique choisi parmi le groupe pipéridine et morpholine, ou dans lequel le groupe phényle est condensé à un noyau aromatique hétérocyclique, de préférence un noyau pyrrole.
 
8. Composé destiné à être utilisé selon l'une quelconque des revendications précédentes, dans lequel R3 est choisi parmi un atome H, un groupe méthyle, C(O)R11 et C(O)O[CR8R9]nOR11, dans lequel R11 est un groupe alkyle de C1 à C4.
 
9. Composé destiné à être utilisé selon l'une quelconque des revendications précédentes, ayant la formule :

dans laquelle R5 est choisi parmi un groupe 1-adamantyle et un groupe CH2-C6H5, ou

dans laquelle R5 est un groupe CH2-C6H5 ; ou


 
10. Composé destiné à être utilisé selon la revendication 1, dans lequel R12 est un groupe aryle choisi parmi

a)

b)

dans lesquels X est choisi parmi un atome F, Cl, Br, un groupe NO2 et CH2OTBDMS, et

c)

dans laquelle X1 et X2 sont indépendamment choisis parmi un atome F, Br et Cl.


 
11. Composé destiné à être utilisé selon la revendication 1, dans lequel R12 est choisi par un atome H, un groupe alcényle, aryle, trihaloalkyle et alkyle de C1 à C6.
 
12. Composé destiné à être utilisé selon l'une quelconque des revendications précédentes, lequel est un médicament pour le traitement et la prévention d'une infection microbienne topique.
 
13. Composé destiné à être utilisé selon l'une quelconque des revendications précédentes, destiné à être utilisé comme un médicament en tant qu'agent antibactérien ou antifongique.
 
14. Procédé de synthèse d'un composé de la formule II, lequel comprend

a) la réaction d'un composé de la formule III

avec un acide carboxylique de la formule R7CO2H ; ou
la réaction d'un composé de la formule III avec i) un acide carboxylique de la formule R7CO2H ou ii) un chlorure acide de la formule R7COCl,
pour former un composé de la formule IV ci-dessous,

et
la réaction du composé de la formule IV avec une base pour convertir le dérivé O-acyle en le dérivé C-acyle correspondant.


 
15. Procédé de synthèse d'un composé de la formule I, dans laquelle Y est un groupe alkyle de C1 à C10, lequel comprend la réaction d'un composé de la formule II avec une amine de la formule R4R5NH.
 
16. Procédé de synthèse d'un composé de la formule la, lequel comprend

a) la réaction d'un composé de la formule III

avec un composé de la formule R5NCO, ou
la réaction d'un composé de la formule IV, avec une amine de la formule R4R5NH, à condition que, quand on fait réagir le composé III avec R5NCO, R4 soit un atome H.


 
17. Composé de la formule I ou II :



dans lesquelles :

X représente un atome O, S ou un groupe SO2,

Y représente un groupe OH ou alkyle de C1 à C10,

R1, R2 et R4 sont chacun indépendamment choisis parmi un atome H et un groupe alkyle de C1 à C6,

R3 est choisi parmi un groupe fonctionnel choisi parmi un atome H, un groupe alkyle de C1 à C6 et un groupe contenant un carbonyle,

R12 est un substituant choisi parmi un atome H, une groupe alcényle, « aryle », trihaloalkyle et alkyle de C1 à C6, dans lequel le groupe « aryle » est un groupe hydrocarbyle aromatique monocyclique ou polycyclique avec au moins 5 atomes de carbone, ou comprend un groupe hydrocarbyle aromatique monocyclique ou polycyclique avec au moins 5 atomes de carbone et un groupe de liaison de la formule -[CR14R15]t qui est couplé au noyau aromatique cyclique ou polycyclique, dans lequel R14 et R15 sont chacun indépendamment choisis parmi un atome H ou un groupe alkyle de C1 ou C2 et t est un nombre entier de 0 à 12, et dans lequel prend place une substitution par l'intermédiaire du groupe de liaison ; et

dans lesquelles R12 est un groupe alcényle ou « aryle », le groupe alcényle ou « aryle » est éventuellement substitué par au moins un groupe halo, nitro, éther et t-butyldiméthylsilyléther (TBDMSO),

R5 et R7 sont chacun un groupe de la formule L1-L2-R6 ou L2-L1-R6,

dans laquelle L1 est un lieur de la formule -[CR8R9]n-, dans laquelle n est un nombre entier de 0 à 12, et Rg et R9 sont chacun indépendamment choisis parmi un atome H ou un groupe alkyle de C1 à C2, et

dans laquelle L2 est absent ou un lieur qui est choisi parmi un atome O, S, un groupe SO, SO2, N(R'), C(O),

C(O)O, OC(O), [O(CR'2)r]s, [(CR'2)rO]s CH(OR'), C(O)N(R'), N(R')C(O), N(R')C(O)N(R'), SO2N(R') ou N(R')SO2, dans lesquels R' et R" sont chacun indépendamment choisis parmi un atome hydrogène et un groupe alkyle de C1 à C2, et dans lesquels r vaut 1 ou 2 et s vaut de 1 à 4, et dans laquelle R6 est un groupe alkyle de C6 à C19 ;

R6 est un groupe phényle qui est éventuellement substitué par au moins un groupe fonctionnel choisi parmi un groupe alkyle, aryle, halo, trihaloalkyle, alcool, thio-alcool, ester, thioester, éther, thioéther, amide, thioamide, urée, thio-urée et un groupe hétérocyclique ;

R6 est un groupe OR13, dans lequel R13 est un groupe alkyle de C1 à C6, ou

R6 est un groupe hétérocyclique qui est éventuellement substitué par au moins un groupe fonctionnel choisi parmi un groupe alcényle, alkyle, aryle et halo ; et

dans lesquelles le groupe alkyle est défini comme un groupe alkyle cyclique et non cyclique.


 
18. Composé selon la revendication 17, dans lequel R6 et un groupe alkyle de C6 à C19; ou R6 est un groupe phényle qui est éventuellement substitué avec au moins un groupe fonctionnel choisi par les groupes alkyle, aryle, halo, trihaloalkyle, alcool, thio-alcool, ester, thioester, éther, thioéther, amide, thioamide, urée, thio-urée et un groupe hétérocyclique.
 
19. Composé selon la revendication 17, dans lequel R6 est un groupe alkyle qui est un système de noyau ponté qui est éventuellement choisi parmi le groupe adamantyle et myrtanyle.
 
20. Composé selon la revendication 17, dans lequel R6 est un groupe phényle qui est substitué par un groupe hétérocyclique choisi parmi le groupe pipéridine et morpholine, ou dans lequel le groupe phényle est condensé à un noyau aromatique hétérocyclique, de préférence un noyau pyrrole.
 
21. Composé selon la revendication 17, dans lequel R12 est choisi par un atome H, un groupe alcényle, aryle, trihaloalkyle et alkyle de C1 à C6.
 
22. Composé selon la revendication 17 ayant la formule :

dans laquelle R5 est choisi parmi le groupe 1-adamantyle et CH2-C6H5, ou

dans laquelle R5 est un groupe CH2-C6H5 ; ou


 
23. Composé de la formule I ou un sel pharmaceutiquement acceptable de celui-ci :

dans lesquelles :

X représente un atome O, S ou un groupe SO2,

Y représente un groupe OH ou alkyle de C1 à C10,

R1, R2 et R4 sont chacun indépendamment choisis parmi un atome H et un groupe alkyle de C1 à C6,

R3 est choisi parmi un groupe fonctionnel choisi parmi un atome H, un groupe alkyle de C1 à C6 et un groupe contenant un carbonyle,

R12 est un substituant choisi parmi un atome H, une groupe alcényle, « aryle », trihaloalkyle et alkyle de C1 à C6, dans lequel le groupe « aryle » est un groupe hydrocarbyle aromatique monocyclique ou polycyclique avec au moins 5 atomes de carbone, ou comprend un groupe hydrocarbyle aromatique monocyclique ou polycyclique avec au moins 5 atomes de carbone et un groupe de liaison de la formule -[CR14R15]t qui est couplé au noyau aromatique cyclique ou polycyclique, dans lequel R14 et R15 sont chacun indépendamment choisis parmi un atome H ou un groupe alkyle de C1 ou C2 et t est un nombre entier de 0 à 12, et dans lequel prend place une substitution par l'intermédiaire du groupe de liaison ; et

dans lesquelles R12 est un groupe alcényle ou « aryle », le groupe alcényle ou « aryle » est éventuellement substitué par au moins un groupe halo, nitro, éther et t-butyldiméthylsilyléther (TBDMSO),

R5 est un groupe de la formule L1-L2-R6 ou L2-L1-R6,

dans lesquelles L1 est un lieur de la formule -[CR8R9]n-, dans laquelle n est un nombre entier de 0 à 12, et R8 et R9 sont chacun indépendamment choisis parmi un atome H ou un groupe alkyle de C1 à C2, et

dans lesquelles L2 est absent ou un lieur qui est choisi parmi un atome O, S, un groupe SO, SO2, N(R'), C(O), C(O)O, OC(O), [O(CR'2)r]s, [(CR'2)rO]s CH(OR'), C(O)N(R'), N(R')C(O), N(R')C(O)N(R'), SO2N(R') ou N(R')SO2, dans lesquels R' et R" sont chacun indépendamment choisis parmi un atome hydrogène et un groupe alkyle de C1 à C2, et dans lesquels r vaut 1 ou 2 et s vaut de 1 à 4, et

dans lesquelles R6 est choisi parmi le groupe OR13, le groupe hétérocyclique et hydrocarbyle de C1 à C25, dans lesquels R13 est un groupe alkyle de C1 à C6, et ledit groupe hétérocyclique et hydrocarbyle est éventuellement substitué par au moins un groupe fonctionnel choisi parmi un groupe alcényle, alkyle, aryle, halo, trihaloalkyle, alcool, céto, S(O)R13, sulfonyle, thio-alcool, ester, thioester, éther, thioéther, amide, thioamide, urée, thio-urée, =O, =S, amine et un groupe hétérocyclique, et

dans lesquelles le groupe alkyle est défini comme un groupe alkyle cyclique et non cyclique.


 
24. Composé selon la revendication 23, dans lequel R12 est choisi par un atome H, un groupe alcényle, aryle, trihaloalkyle et alkyle de C1 à C6.
 






Cited references

REFERENCES CITED IN THE DESCRIPTION



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Patent documents cited in the description




Non-patent literature cited in the description