(19)
(11)EP 2 907 805 B1

(12)EUROPEAN PATENT SPECIFICATION

(45)Mention of the grant of the patent:
22.07.2020 Bulletin 2020/30

(21)Application number: 13845800.5

(22)Date of filing:  17.09.2013
(51)International Patent Classification (IPC): 
C07D 231/12(2006.01)
A61K 31/4439(2006.01)
A61K 31/5377(2006.01)
A61P 43/00(2006.01)
C07D 403/06(2006.01)
A61K 31/4155(2006.01)
A61K 31/496(2006.01)
A61P 25/28(2006.01)
C07D 401/14(2006.01)
C07D 405/06(2006.01)
(86)International application number:
PCT/JP2013/074998
(87)International publication number:
WO 2014/057772 (17.04.2014 Gazette  2014/16)

(54)

NOVEL PYRAZOLE DERIVATIVE

NEUARTIGES PYRAZOLDERIVAT

NOUVEAU DÉRIVÉ DE PYRAZOLE


(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30)Priority: 10.10.2012 JP 2012225273

(43)Date of publication of application:
19.08.2015 Bulletin 2015/34

(73)Proprietor: Green Tech Co., Ltd.
Tarumi Tsu-shi Mie 514-0821 (JP)

(72)Inventors:
  • SUGIMOTO, Hachiro
    Kyoto-shi Kyoto 6048093 (JP)
  • OKUDA, Michiaki
    Kyoto-shi Kyoto 6068414 (JP)
  • NAKAYAMA, Shinichi
    Nishinomiya-shi, Hyogo 6638114 (JP)
  • INOUE, Yoshikazu
    Osaka-shi, Osaka 5530006 (JP)
  • SAKATA, Yoko
    Osaka-shi, Osaka 5380042 (JP)
  • FUJITA, Yuki
    Otsu-shi, Shiga 5200052 (JP)
  • TOKIZANE, Rie
    Nishinomiya-shi, Hyogo 6638184 (JP)

(74)Representative: Teipel, Stephan et al
Lederer & Keller Patentanwälte Partnerschaft mbB Unsöldstrasse 2
80538 München
80538 München (DE)


(56)References cited: : 
WO-A1-2008/066151
WO-A2-03/105751
WO-A2-2010/045395
JP-A- S6 322 079
WO-A1-2009/145219
WO-A2-2006/033943
CN-A- 101 570 512
JP-A- 2012 229 208
  
  • AMOLINS M W ET AL: "Synthesis and evaluation of electron-rich curcumin analogues", BIOORGANIC & MEDICINAL CHEMISTRY, PERGAMON, GB, vol. 17, no. 1, 29 October 2008 (2008-10-29), pages 360-367, XP025876006, ISSN: 0968-0896, DOI: 10.1016/J.BMC.2008.10.057 [retrieved on 2008-10-29]
  • SELVAM C ET AL: "Design, synthesis, biological evaluation and molecular docking of curcumin analogues as antioxidant, cyclooxygenase inhibitory and anti-inflammatory agents", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, PERGAMON, AMSTERDAM, NL, vol. 15, no. 7, 2005, pages 1793-1797, XP004789383, ISSN: 0960-894X, DOI: 10.1016/J.BMCL.2005.02.039
  • OHTSU H ET AL: "Antitumor Agents. 217. Curcumin Analogues as Novel Androgen Receptor Antagonists with Potential as Anti-Prostate Cancer Agents", JOURNAL OF MEDICINAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 45, no. 23, 12 October 2002 (2002-10-12), pages 5037-5042, XP002536788, ISSN: 0022-2623, DOI: 10.1021/JM020200G
  • RAJESHWAR NARLAWAR ET AL.: 'Curcumin-derived pyrazoles and isoxazoles: swiss army knives or blunt tools for Alzheimer's disease?' CHEMMEDCHEM vol. 3, no. 1, 2008, pages 165 - 172, XP002633816
  • KONG, BYUNGMUN ET AL.: 'Protectors of oxidative stress inhibit Abeta (1-42) aggregation in vitro' BULLETIN OF THE KOREAN CHEMICAL SOCIETY vol. 23, no. 12, 2002, pages 1773 - 1777, XP009147469
  
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).


Description

Technical Field



[0001] The present invention relates to novel pyrazole derivatives useful for the prevention or treatment of diseases such as dementia and Alzheimer's disease and a pharmaceutical composition containing the same.

Background Art



[0002] Senile dementia has become a serious medical and social problem along with the rapid aging of society in recent years and the development of effective anti-dementia drugs has been greatly desired. There are already very many studies on Alzheimer's disease but the cause of the disease has not been clearly defined. Drugs such as acetylcholinesterase inhibitors including donepezil (Aricept (registered trademark)), galantamine (Reminyl) and rivastigmine (Exelon/Rivastach), and NMDA receptor antagonists including memantine hydrochloride (Memary) have been used as Alzheimer's therapeutic drugs. These drugs are very useful as symptomatic therapy but are not drugs for fundamental treatment.

[0003] Alzheimer's disease is considered to be caused by aggregation of Aβ, aggregation of tau, and the like. Hence, a substance that inhibits aggregation of these proteins could be used as a fundamental therapeutic drug for Alzheimer's disease.

[0004] Yang et al. have reported that curcumin has an Aβ aggregation inhibitory activity, a disaggregation activity on Aβ aggregate, and the like (Non Patent Literature 1). The inventors of the present invention have revealed that curcumin and its derivatives have an inhibitory activity against secretase, which is involved in the generation of Aβ (Patent Literature 1 and 2). Narlawar et al. have synthesized curcumin derivatives by replacing the 1,3-dicarbonyl moiety with a pyrazole ring and reported that these compounds have a tau aggregation inhibitory activity (Non Patent Literature 2).

Citation List


Patent Literature



[0005] 

Patent Literature 1: WO 2008/066151

Patent Literature 2: WO 2009/145219


Non Patent Literature



[0006] 

Non Patent Literature 1: Fusheng Yang et al., J. Biol. Chem. 2005, Feb 18; 280 (7) 5892-5901

Non Patent Literature 2: Rajeshwar Narlawar et al., ChemMedChem 2008, 3, 165-172 Further reference is made to Kong, Byungmun et al., "Bulletin of the Korean Chemical Society", vol. 23, no 12, 2002, pages 1773-1777.


Summary of Invention


Technical Problem



[0007] As described in the above literature, a curcumin derivative can be a promising candidate for a fundamental therapeutic drug for Alzheimer's disease. Under this technical background, an object of the present invention is thus to provide a novel therapeutic means for Alzheimer's disease.

Solution to Problem



[0008] The inventors succeeded in creating a novel compound based on technical ideas that are distinct from those forming the basis of known compounds and found that the compound has an excellent pharmacological activity. The inventors further conducted extensive studies to complete the present invention.

[0009] That is, as a result of the extensive investigations to solve the above problems, the inventors succeeded in synthesizing curcumin derivatives by replacing the 1,3-dicarbonyl moiety of curcumin with a pyrazole ring and replacing at least one of the 4-hydroxy-3-methoxyphenyl groups at both ends with a substituent, and found that these novel compounds have a potent tau aggregation inhibitory activity. The inventors also found that these derivatives has a high brain penetration and also possesses a β-secretase inhibitory activity and an Aβ aggregation inhibitory activity.

[0010] A curcumin derivative in which the 1,3-dicarbonyl moiety of curcumin is replaced with a pyrazole ring is described in Non Patent Literature 2 etc. A curcumin derivative in which one of the 4-hydroxy-3-methoxyphenyl groups at both ends of curcumin is replaced with a substituent is described in Patent Literature 1 and 2, etc. However, the derivatives of the present invention which have a pyrazole ring and in which at least one of the 4-hydroxy-3-methoxyphenyl groups at both ends is replaced with a substituent are a novel compounds having a chemical structure that is unique to the present invention and distinct from those of compounds disclosed in known literature.

[0011] The tau aggregation inhibitory activity of a curcumin derivative is described in Non Patent Literature 2. In this literature, the nitrogen atom at position 1 of the pyrazole ring is replaced with various groups, and the tau aggregation inhibitory activities of the derivatives significantly vary with the groups introduced into the ring. However, no modification was made to the benzene rings at both ends and thus all the synthesized derivatives have the 4-hydroxy-3-methoxyphenyl groups as curcumin does. Therefore, the person skilled in the art who has read Non Patent Literature 2 is expected to consider that the group introduced into the pyrazole ring plays an important role for the tau aggregation inhibitory activity and that the 4-hydroxy-3-methoxyphenyl groups at both ends are irrelevant to the tau aggregation inhibitory activity. Thus the person skilled in the art would not attempt to replace the 4-hydroxy-3-methoxyphenyl groups with a substituent.

[0012] That is, the present invention relates to
  1. [1] A compound represented by formula:

























































    or

    or a pharmaceutically acceptable salt thereof
  2. [2] The compound according to the above [1] represented by formula





    or

    or a pharmaceutical acceptable salt thereof.
  3. [3] The compound according to the above [1] or [2] or a salt thereof as an active ingredient.
  4. [4] The pharmaceutical composition according to the above [3] for use in the prevention or treatment of a disease selected from the group consisting of Alzheimer's disease, familial Alzheimer's disease, sporadic Alzheimer's disease, senile dementia, Down syndrome, Parkinson's disease, Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, diabetic neuropathy, Huntington's chorea, and multiple sclerosis.
  5. [5] The compound according to the above [1] or [2] or a salt thereof for use in the prevention or treatment of a disease selected from the group consisting of Alzheimer's disease, familial Alzheimer's disease, sporadic Alzheimer's disease, senile dementia, Down syndrome, Parkinson's disease, Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, diabetic neuropathy, Huntington's chorea, and multiple sclerosis.
  6. [6] The pharmaceutical composition for use according to the above [4], wherein the disease is Alzheimer's disease.
  7. [7] The compound according to the above [1] or [2] or a salt thereof for use in the prevention or treatment of Alzheimer's disease.
  8. [8] An oral or parenteral preparation comprising the compound according to 8 the above [1] or [2] or a salt thereof and one or more pharmacologically acceptable carriers.

Advantageous Effects of Invention



[0013] The compound of the present invention is remarkably excellent in a tau aggregation inhibitory activity, a β-secretase inhibitory activity, an Aβ aggregation inhibitory activity, and/or the like, and is thus useful as a therapeutic drug for Alzheimer's disease and the like. The compound of the present invention also has a high brain penetration, and thus a highly efficient therapeutic drug can be provided.

Description of Embodiments



[0014] The present invention will be described in detail below.

[0015] The term "halogen atom" used herein means a fluorine atom, a chlorine atom, a bromine atom, and an iodine atom. The term "C1-6 alkyl group" means a linear or branched alkyl group of 1 to 6 carbon atoms, and examples thereof include methyl, ethyl, n-propyl, isopropyl, n-butyl, isobutyl, sec-butyl, tert-butyl, n-pentyl, neopentyl, and n-hexyl groups. The term "halo C1-6 alkyl group" means a linear or branched 1- to 6-carbon alkyl group substituted with one or more halogen atoms that may be the same or different, and examples thereof include trifluoromethyl, difluoromethyl, perfluoroethyl, hexafluoroisopropyl, perfluoroisopropyl, chloromethyl, bromomethyl, 1-bromoethyl, and 2,3-dibromopropyl groups.

[0016] The term "C1-6 alkoxy group" means a linear or branched alkoxy group of 1 to 6 carbon atoms, and examples thereof include methoxy, ethoxy, n-propoxy, isopropoxy, n-butoxy, sec-butoxy, tert-butoxy, n-pentyloxy, isopentyloxy, neopentyloxy, and n-hexyloxy groups. The term "halo C1-6 alkoxy group" means a linear or branched 1- to 6-carbon alkoxy group substituted with one or more halogen atoms that may be the same or different, and examples thereof include trifluoromethoxy, difluoromethoxy, perfluoroethoxy, perfluoroisopropoxy, chloromethoxy, bromomethoxy, 1-bromoethoxy, and 2,3-dibromopropoxy groups.

[0017] The term "C1-6 acyl group" means a linear or branched acyl group of 1 to 6 carbon atoms, and examples thereof include formyl, acetyl, propionyl, butyryl, 2-methylpropionyl, pivaloyl, pentanoyl, 3-methylbutyryl, and hexanoyl groups. The term "halo C1-6 acyl group" means a linear or branched 1- to 6-carbon acyl group substituted with one or more halogen atoms that may be the same or different, and examples thereof include chloroformyl, bromoformyl, dichloroacetyl, dibromoacetyl, and trifluoroacetyl groups.

[0018] The term "C1-6 alkylsulfonyl group" means a linear or branched alkylsulfonyl group of 1 to 6 carbon atoms, and examples thereof include methylsulfonyl, ethylsulfonyl, n-propylsulfonyl, isopropylsulfonyl, n-butylsulfonyl, sec-butylsulfonyl, tert-butylsulfonyl, n-pentylsulfonyl, isopentylsulfonyl, and n-hexylsulfonyl groups. The term "halo C1-6 alkylsulfonyl group" means a linear or branched 1- to 6-carbon alkylsulfonyl group substituted with one or more halogen atoms that may be the same or different, and examples thereof include trifluoromethylsulfonyl, difluoromethylsulfonyl, perfluoroethylsulfonyl, perfluoroisopropylsulfonyl, chloromethylsulfonyl, bromomethylsulfonyl, 1-bromoethylsulfonyl, and 2,3-dibromopropylsulfonyl groups.

[0019] The term "mono- or di (C1-6 alkyl) amino group" means an amino group mono- or di-substituted with the above C1-6 alkyl group, and examples thereof include methylamino, ethylamino, propylamino, isopropylamino, butylamino, sec-butylamino, tert-butylamino, dimethylamino, diethylamino, ethylmethylamino, dipropylamino, methylpropylamino, and diisopropylamino groups. The term "C1-6 alkylthio group" means a linear or branched alkylthio group of 1 to 6 carbon atoms, and examples thereof include methylthio, ethylthio, n-propylthio, isopropylthio, n-butylthio, sec-butylthio, tert-butylthio, n-pentylthio, isopentylthio, and n-hexylthio groups. The term "C1-6 acylamino group" means an amino group substituted with the above C1-6 acyl group, and examples thereof include acetylamino and propionylamino groups.

[0020] The term "C2-6 alkenyl group" means a linear or branched alkenyl group of 2 to 6 carbon atoms, and examples thereof include vinyl, propenyl, and butenyl groups. The term "C2-6 alkenyloxy group" means a linear or branched alkenyloxy group of 2 to 6 carbon atoms, and examples thereof include propenyloxy, butenyloxy, and pentenyloxy groups. The term "mono- or di(C2-6 alkenyl)amino group" means an alkenyl amino group mono- or di-substituted with the above C2-6 alkenyl group, and examples thereof include vinylamino, propenylamino, butenylamino, and divinylamino groups.

[0021] The term "C2-6 alkenylthio group" means a linear or branched alkenylthio group of 2 to 6 carbon atoms, and examples thereof include vinylthio, 1-propenylthio, isopropenylthio, 1-butenylthio, 2-butenylthio, and 2-methylallylthio groups. The term "nitrogen-containing heterocyclic group" means a saturated or unsaturated heterocyclic group containing one or more nitrogen atoms and optionally containing one or more oxygen and/or sulfur atoms, and examples thereof include pyridyl, pyrimidyl, pyrazyl, morpholino, 4-(substituted)piperazino (e.g., 4-methylpiperazino etc.), 1,1-dioxo-1,4-thiazinan-4-yl, piperidino, pyrrolidino, thiazolyl, azepan-1-yl, and 4-(substituted)-1,4-diazepano (e.g., 4-methyl-1,4-diazepano) groups.

[0022] The compounds as defined above under item [1] have the following general formula (I) in common:



[0023] In the above general formula (I), R1 represents a hydrogen atom, a halogen atom, a hydroxy group, a nitro group, an amino group, a cyano group, a C1-6 alkyl group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a C1-6 alkoxy group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a mono- or di(C1-6 alkyl)amino group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a C1-6 alkylthio group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a C1-6 alkylsulfonyl group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a C1-6 acyl group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a C1-6 acylamino group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a C2-6 alkenyl group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a C2-6 alkenyloxy group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a mono- or di(C2-6 alkenyl)amino group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a C2-6 alkenylthio group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group, a carbamoyl group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a morpholino group, and a pyridyl group.

[0024] In the above general formula (I), R2 represents a group represented by the following general formula (II):



[0025] In the above general formula (II), m and n each represent an integer of 0 or 1. A represents -O-, -NH-,-S-, -SO-, -SO2-,, and preferably represents -O-, -SO2-,. Y represents a C1-6 alkylene group, a C2-6 alkenylene group, a C2-6 alkynylene group, and preferably represents a methylene group, an ethylene group,. R3 represents a nitrogen-containing heterocyclic group unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a C1-6 alkyl group, a halo C1-6 alkyl group, a C1-6 alkoxy group, a halo C1-6 alkoxy group, a C1-6 acyl group, a halo C1-6 acyl group, a C1-6 alkylsulfonyl group and a halo C1-6 alkylsulfonyl group, a C1-6 alkoxy group unsubstituted or substituted with one or more substituents selected from the group consisting of a methoxy group, an ethoxy group and a 2-methoxyethoxy group, a mono- or di(C1-6 alkyl)amino group unsubstituted or substituted with one or more substituents selected from the group consisting of a C1-6 alkoxy group, a halo C1-6 alkoxy group, a C1-6 alkylsulfonyl group and a halo C1-6 alkylsulfonyl group, a mono- or di(C2-6 alkenyl)amino group unsubstituted or substituted with one or more substituents selected from the group consisting of a C1-6 alkoxy group, a halo C1-6 alkoxy group, a C1-6 alkylsulfonyl group and a halo C1-6 alkylsulfonyl group, or a carbamoyl group unsubstituted or substituted with one or more substituents selected from the group consisting of a C1-6 alkyl group and a C1-6 alkoxy group.

[0026] In the above general formula (I), R1 and R2 may be joined together to form a ring. In this case, R1 and R2 form a fused ring or the like together with the benzene ring to which R1 and R2 are attached. Examples of the fused ring include a 1,3-benzodioxole group optionally having one or more substituents, a 1,4-benzodioxan-5-yl group optionally having one or more substituents, a 1,4-benzodioxan-6-yl group optionally having one or more substituents, a 1,4-benzodioxin-6-yl group optionally having one or more substituents, a 1,4-benzodioxin-2-yl group optionally having one or more substituents, a quinolino group optionally having one or more substituents, , and an indolyl group optionally having one or more substituents.

[0027] In the above general formula (I), Ar represents a homocyclic or heterocyclic group selected from the group consisting of pyrrolyl, imidazolyl, furyl, pyridyl, pyrazyl groups, and a bicyclic group having a benzene skeleton, and which are unsubstituted or substituted with one or more substituents selected from the group consisting of a halogen atom, a hydroxy group, a nitro group, an amino group, a C1-6 alkyl group, a halo C1-6 alkyl group, a C1-6 alkoxy group, a halo C1-6 alkoxy group, a C1-6 acyl group, a halo C1-6 acyl group, a C1-6 alkylsulfonyl group, and a halo C1-6 alkylsulfonyl group, or Ar is a phenyl group substituted with an ethoxy, morpholinomethoxy, morpholinoethoxy, pyridylmethoxy, or pyridylethoxy group, or a salt thereof..

[0028] A salt of the compound as defined above under item [1] is also encompassed in the present invention. The salt is preferably a pharmacologically acceptable salt and examples thereof include hydrohalic acid salts such as hydrofluoride, hydrochloride, hydrobromide, and hydroiodide; inorganic acid salts such as sulfate, nitrate, perchlorate, phosphate, carbonate, and bicarbonate; organic carboxylic acid salts such as acetate, oxalate, maleate, tartrate, and fumarate; organic sulfonic acid salts such as methanesulfonate, trifluoromethanesulfonate, ethanesulfonate, benzenesulfonate, toluenesulfonate, and camphorsulfonate; amino acid salts such as aspartate and glutamate; amine salts such as trimethylamine salt, triethylamine salt, procaine salt, pyridine salt, and phenethylbenzylamine salt; alkali metal salts such as sodium salt and potassium salt; alkaline earth metal salts such as magnesium salt and calcium salt; etc. In view of the water solubility, oral absorbability, efficacy, and the like, preferred are hydrochloride and oxalate.

The compound represented of the invention or pharmaceutically acceptable salt thereof is preferably a compound as defined above under item [2]



[0029] The compound of the invention can be produced by known methods described in, for example, Rajeshwar Narlawar et al., ChemMedChem 2008, 3, 165-172, WO 2008/066151, WO 2009/145219, or the like, or by any combination of the methods described in the literature, or by a method known per se or an equivalent method thereto. Specifically, the compound can be produced by, for example, the following Steps 1 and 2, but the production method is not limited thereto.



[0030] Ar1 in the above formula is either the phenyl ring substituted with R1 and R2 or a ring represented by Ar in the general formula (I) as described above, and Ar2 is the other one. In Step 1, an aldehyde represented by the general formula (A) is allowed to react with a compound represented by the general formula (B) in the presence of a solvent and a catalyst to give a diketone represented by the general formula (C).

[0031] The solvent used in the reaction is not particularly limited as long as it does not inhibit the reaction, and examples thereof include ethyl acetate, N,N-dimethylacetamide, N,N-dimethylformamide, 1-methyl-2-pyrrolidone, dimethylsulfoxide, tetrahydrofuran, acetonitrile, etc. These solvents may be used alone or in combination of two or more kinds thereof at a given mixing ratio.

[0032] The catalyst used in the reaction is also not particularly limited. Examples thereof include bases such as a primary amine and a secondary amine, and specific examples thereof include n-butylamine, ethanolamine, piperidine, morpholine, etc.

[0033] In Step 1, a water scavenger may be added in order to capture the water produced by the reaction. Examples of the water scavenger include an alkyl borate, an alkyl phosphate, an orthoester, etc., and specific examples thereof include trimethyl orthoformate and tri-n-butyl borate.

[0034] In Step 1, the quantitative ratio of the aldehyde represented by the general formula (A) and the compound represented by the general formula (B) is not particularly limited as long as the reaction proceeds. However, in view of the reaction efficiency and the like, the amount of the compound is preferably 0.5 to 10 mol, more preferably 0.5 to 2 mol, relative to 1 mol of the aldehyde.

[0035] In Step 1, the reaction temperature is not particularly limited as long as the reaction proceeds. However, in view of the reaction efficiency and the like, the reaction temperature is preferably 0 to 200°C, more preferably 50 to 100°C.

[0036] In Step 1, the reaction duration is not particularly limited as long as the reaction proceeds. However, in view of the production efficiency and the like, the reaction duration is preferably 0.5 to 48 hours, more preferably 1 to 24 hours.

[0037] The aldehyde represented by the general formula (A) and the compound represented by the general formula (B) that are used in Step 1 may be commercially available products or those synthesized by a known method (for example, the method described in WO 2008/066151 or WO 2009/145219) or other methods.

[0038] In Step 2, a diketone represented by the general formula (C) is allowed to react with a hydrazine in the presence of a solvent to give a compound represented by the general formula (I).

[0039] The hydrazine used in the reaction is not particularly limited and examples thereof include hydrazine monohydrate, hydrazine aqueous solution, anhydrous hydrazine, hydrazine acetate, hydrazine monohydrochloride, hydrazine dihydrochloride, a derivative thereof, etc.

[0040] The solvent used in the reaction is not particularly limited as long as it does not inhibit the reaction and examples thereof include protic solvents such as acetic acid, methanol, ethanol, and water; non-protic solvents such as ethyl acetate, toluene, tetrahydrofuran, methylene chloride, chloroform, etc. These solvents may be used alone or, if desired, in combination of two or more kinds thereof at a given mixing ratio.

[0041] In Step 2, the quantitative ratio of the diketone represented by the general formula (C) and the hydrazine is not particularly limited as long as the reaction proceeds. However, in view of the reaction efficiency and the like, the amount of the hydrazine is preferably 1 to 50 mol, more preferably 2 to 10 mol, relative to 1 mol of the diketone.

[0042] In Step 2, the reaction temperature is not particularly limited as long as the reaction proceeds. However, in view of the reaction efficiency and the like, the reaction temperature is preferably 20 to 120°C, more preferably 50 to 80°C.

[0043] In Step 2, the reaction duration is not particularly limited as long as the reaction proceeds. However, in view of the production efficiency and the like, the reaction duration is preferably 0.2 to 24 hours, more preferably 0.5 to 6 hours.

[0044] The compound of the present invention may be administered to a subject, alone or in combination with one or more of other compounds of the present invention, or with one or more compounds other than the compounds of the present invention. The compound of the present invention may be administered as a preparation or pharmaceutical composition comprising one or more pharmacologically acceptable carriers. The effective dosage of the compound of the present invention as an active ingredient and frequency of administration may vary with the dosage form, the age, body weight, symptoms, etc. of the patient, and the like, but the daily dosage is usually about 0.01 to 100 mg/kg, more preferably about 1 to 50 mg/kg.

[0045] The subject is not particularly limited and examples thereof include mammals such as a human, a monkey, a hamadryas baboon, a chimpanzee, a mouse, a rat, a guinea pig, a hamster, a rabbit, a cat, a dog, a sheep, a goat, a pig and a cattle.

[0046] The route of administration of the preparation or pharmaceutical composition comprising the compound of the present invention as an active ingredient is not particularly limited, and the preparation or pharmaceutical composition may be administered orally or parenterally by a usual method. Examples of the parenteral administration include rectal administration, nasal administration, transpulmonary administration, dermal administration, and injection administration (e.g., intravenous administration, intraspinal administration, epidural administration, intramuscular administration, subcutaneous administration, intraperitoneal administration, intraarterial administration, intraarticular administration, intracardiac administration, intracystic administration, intracutaneous administration, intralesional administration, intraocular administration, intrathoracic administration, subarachnoid administration, intrauterine administration, intraventricular administration, etc.).

[0047] The form of the preparation or pharmaceutical composition comprising the compound of the present invention as an active ingredient is not particularly limited, and examples thereof include oral or parenteral preparations such as tablets, powders, granules, capsules, oral solutions, emulsions, elixirs, lemonades, suspensions, syrups, oromucosal tablets, oral jellies, inhalations, suppositories, injections, ointments, ophthalmic ointments, ophthalmic preparations, nasal preparations, ear preparations, patches, solutions for external application.

[0048] The dosage of the preparation or pharmaceutical composition of the present invention can be determined as appropriate depending on the severity of the symptom, the age, sex, and body weight of the patient, the route of administration, the type of the salt, the specific disease, etc.

[0049] Since the compound of the present invention has a tau aggregation inhibitory activity, a β-secretase inhibitory activity, and/or an Aβ aggregation inhibitory activity, the compound of the present invention is effective in preventing and treating diseases in which tau, β-secretase or Aβ is involved, such as Alzheimer's disease (familial Alzheimer's disease and sporadic Alzheimer's disease), senile dementia, Down syndrome, Parkinson's disease, Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, diabetic neuropathy, Huntington's chorea, multiple sclerosis, etc. Among these nervous diseases, the compound of the present invention is especially effective in preventing and treating Alzheimer's disease.

[0050] The compound of the present invention can be formulated by a commonly used method into dosage forms such as tablets, powders, granules, capsules, oral solutions, emulsions, elixirs, lemonades, suspensions, syrups, oromucosal tablets, oral jellies, inhalations, suppositories, injections, ointments, ophthalmic ointments, ophthalmic preparations, nasal preparations, ear preparations, patches, solutions for external application, etc. For the formulation into such dosage forms, excipients, binders, lubricants, colorants and flavor modifiers that are usually used for the formulation of medicines can be used, and as needed stabilizers, emulsifiers, absorption enhancers, surfactants, pH adjusters, preservatives, antioxidants, and/or the like can also be used. Thus, ingredients that are usually used as raw materials of a pharmaceutical preparation may be mixed with the compound and formulated into a dosage form by a conventional method.

[0051] For example, in the production of oral preparations, a crystalline or amorphous compound of the present invention is mixed with excipients, and as needed with additives such as binders, disintegrators, lubricants, colorants, flavor modifiers, etc., and formed into powders, fine granules, granules, tablets, coated tablets, capsules, etc. by a conventional method. Examples of the additives include animal and vegetable fats and oils such as soybean oil, beef tallow, and synthetic glyceride; hydrocarbons such as liquid paraffin, squalane, and hard paraffin; ester oils such as octyldodecyl myristate and isopropyl myristate; higher alcohols such as cetostearyl alcohol and behenyl alcohol; silicone resins; silicone oils; surfactants such as polyoxyethylene fatty acid ester, sorbitan fatty acid ester, glycerin fatty acid ester, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene hardened castor oil, and polyoxyethylene-polyoxypropylene block copolymer; water soluble polymers such as hydroxyethyl cellulose, hydroxypropyl methylcellulose, hydroxypropyl methylcellulose phthalate, polyacrylate, carboxy vinyl polymer, polyethylene glycol, polyvinylpyrrolidone, and methylcellulose; lower alcohols such as ethanol and isopropanol; polyalcohols such as glycerin, propylene glycol, dipropylene glycol, and sorbitol; sugars such as glucose and sucrose; inorganic powders such as anhydrous silicic acid, magnesium aluminum silicate, and aluminium silicate; purified water; etc.

[0052] Examples of the excipients include lactose, corn starch, saccharose, glucose, mannitol, sorbitol, crystalline cellulose, silicon dioxide, etc. Examples of the binders include polyvinyl alcohol, polyvinyl ether, methylcellulose, ethylcellulose, gum arabic, tragacanth, gelatin, shellac, hydroxypropyl methylcellulose, hydroxypropyl cellulose, polyvinylpyrrolidone, polypropylene glycol-polyoxyethylene block polymer, meglumine, etc. Examples of the disintegrators include starch, agar, gelatin powder, crystalline cellulose, calcium carbonate, sodium bicarbonate, calcium citrate, dextrin, pectin, carboxymethyl cellulose calcium, etc. Examples of the lubricants include magnesium stearate, talc, polyethylene glycol, silica, hydrogenated vegetable oil, etc. Examples of the colorants include a colorant that is approved as additives to a medicine, etc. Examples of the flavor modifiers include cocoa powder, menthol, aromatic powder, mentha oil, borneol, cinnamon powder, etc.

[0053] Needless to say, in the production of the tablets or granules, coating of the tablets or granules with a sugar or the like may be performed as needed. In the production of solutions such as syrups, emulsions, elixirs, lemonades, suspensions, and injections, the compound of the present invention can be mixed as needed with further additives such as pH adjusters, solubilizers, emulsifiers, disintegrators, isotonic agents, solubilization assisting agents, stabilizers, and the like, and formed into such solutions by a conventional method.

[0054] In the production of external medicines, the production method is not limited and the production can be carried out by a conventional method. That is, for the formulation of external medicines, various types of raw materials that are usually used for medicines, quasi drugs, cosmetics, or the like can be used as a base ingredient. Specific examples of the base ingredients to be used include animal and vegetable oils, mineral oils, ester oils, waxes, higher alcohols, fatty acids, silicone oils, surfactants, phospholipids, alcohols, polyalcohols, water soluble polymers, clay minerals, water-insoluble natural or synthetic polymers such as resins, plastics and rubbers, purified water, etc. Further, pH adjusters, antioxidants, chelating agents, antibacterial and antifungal agents, colorants, flavors, and/or the like can be added as needed. The base ingredients of the external medicines of the present invention are not limited to the above ingredients. As needed, other ingredients can be added and examples thereof include ingredients having a differentiation inducing activity, blood flow increasing agents, bactericides, anti-inflammatories, cell activators, vitamins, amino acids, moisturizers, keratolytic agents, etc. The amount of the base ingredients to be added is determined so that the concentration will be a usual base ingredient concentration in the production of external medicines.

[0055] The compound of the present invention may be provided as a food or drink, a feed, or a food additive.

[0056] The food or drink of the present invention may contain one or more types of food additives commonly used in food or drink, and examples of the food additives include sweeteners, colorants, preservatives, thickening stabilizers, antioxidants, color fixatives, bleaching agents, antifungal agents, gum bases, bittering agents, enzymes, brighteners, acidulants, seasonings, emulsifiers, fortifiers, processing aids, flavors, and spice extracts. The food or drink of the present invention includes health foods, functional foods, foods for specified health use, foods for babies, foods for small children, foods for pregnant women and nursing mothers, foods for elderly people, and foods for sick people.

[0057] The form of the food or drink of the present invention is not particularly limited. Specific examples thereof include so-called dietary supplements in forms of tablets, granules, powders, energy drinks, or the like. Other examples thereof include drinks, such as tea drink, refreshing drink, carbonated drink, nutritional drink, fruit juice, and lactic drink; noodles, such as buckwheat noodle, wheat noodle, Chinese noodle, and instant noodle; sweets and bakery products, such as drop, candy, gum, chocolate, snack, biscuit, jelly, jam, cream, pastry, and bread; fishery or livestock products, such as fish sausage, ham, and sausage; dairy products, such as processed milk and fermented milk; fats, oils and processed foods thereof, such as vegetable oil, oil for deep frying, margarine, mayonnaise, shortening, whipped cream, and dressing; seasonings, such as sauce and dipping sauce; retort pouch foods, such as curry, stew, rice-bowl cuisine, porridge, and rice soup; and frozen desserts, such as ice cream, sherbet, and shaved ice.

[0058] The amount of intake of the food or drink of the present invention is not particularly limited, and may be determined depending on the form of the food or drink, the age, sex, condition, and the like of the subject who is to take the food or drink, and other conditions.

[0059] The compound of the present invention or a salt thereof can be used for a therapeutic method for a disease in which tau, β-secretase or Aβ is involved. Specific examples of the method include the following (a) to (c):
  1. (a) a therapeutic method for a disease in which tau is involved, the method comprising the step of administering a compound of the invention or a salt thereof to a patient with a disease in which tau is involved;
  2. (b) a therapeutic method for a disease in which β-secretase is involved, the method comprising the step of administering a compound represented by the general formula (I) or a salt thereof to a patient with a disease in which β-secretase is involved; and
  3. (c) a therapeutic method for a disease in which Aβ is involved, the method comprising the step of administering a compound of the invention or a salt thereof to a patient with a disease in which Aβ is involved.


[0060] The diseases in which tau, β-secretase or Aβ is involved preferably include diseases of which the onset mechanism involves tau, β-secretase or Aβ. Specific examples of such diseases include Alzheimer's disease (familial Alzheimer's disease and sporadic Alzheimer's disease), senile dementia, Down syndrome, Parkinson's disease, Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, diabetic neuropathy, Huntington's chorea, multiple sclerosis, etc. Among these, preferred is Alzheimer's disease.

[0061] The route of administration, dosage form, and dosage of the compound of the present invention, and the subject to which the compound is to be administered may be the same as those described above for the preparation comprising the compound of the present invention.

[0062] The compound of the present invention can also be used for a method for inhibiting tau aggregation, a method for inhibiting β-secretase, and a method for inhibiting Aβ aggregation. Specific examples of the method include the following (d) to (i):

(d) a method for inhibiting tau aggregation, the method comprising the step of administering a compound of the invention or a salt thereof to a mammal including human, thereby inhibiting tau aggregation in the body of the mammal including human;

(e) a method for inhibiting tau aggregation, the method comprising the step of bringing a compound of the invention or a salt thereof in contact with tau;

(f) a method for inhibiting β-secretase, the method comprising the step of administering a compound of the invention or a salt thereof to a mammal including human, thereby inhibiting β-secretase in the living human body;

(g) a method for inhibiting β-secretase, the method comprising the step of bringing a compound of the invention or a salt thereof in contact with β-secretase;

(h) a method for inhibiting Aβ aggregation, the method comprising the step of administering a compound of the invention or a salt thereof to a mammal including human, thereby inhibiting Aβ aggregation in the body of the mammal including human; and

(i) a method for inhibiting Aβ aggregation, the method comprising the step of bringing a compound of the invention or a salt thereof in contact with Aβ.



[0063] The salt in the above methods may be a pharmacologically acceptable salt.

[0064] The present invention also includes, as one embodiment, use of the compound of the present invention or a salt thereof in the production of a prophylactic or therapeutic preparation for a disease in which tau, β-secretase or Aβ is involved. The disease in which tau, β-secretase or Aβ is involved may be the same as those described above, and is preferably Alzheimer's disease.

[0065] The present invention also includes, as another embodiment, the compound of the present invention or a salt thereof for use in the prevention or treatment of a disease in which tau, β-secretase or Aβ is involved. The disease in which tau, β-secretase or Aβ is involved may be the same as those described above, and is preferably Alzheimer's disease.

Examples



[0066] The present invention will be described in further detail with reference to Examples etc.

[0067] In Examples, the compound of the general formula (I) is detected as a mixture of two tautomers represented by the general formulas (I) and (I') shown below, depending on the measurement conditions of 1H-NMR. The two tautomers are isomers of the same substance. Therefore, the synthetic compounds in Examples can be named based on either the general formula (I) or the general formula (I').



[0068] A compound represented by the above general formula (C) is an important intermediate in the present invention. The compound of the general formula (C) can exist in tautomeric forms including a keto form and an enol form, and these tautomeric forms are the same substance. Therefore, such an intermediate can be named based on any of the general formula (C), the general formula (C'), and the general formula (C").



[0069] The following abbreviations are also used herein.
Table 1
AbbreviationReagent, solvent, etc.
AcOEt Ethyl acetate
AcOH Acetic acid
CHCl3 Chloroform
IBCF Isobutyl chloroformate
K2CO3 Potassium carbonate
LAH Lithium aluminium hydride
MgSO4 Magnesium sulfate
Na2SO4 Sodium sulfate
NaHCO3 Sodium hydrogen carbonate
NaOH Sodium hydroxide
PTLC Preparative thin-layer chromatography
THF Tetrahydrofuran

Example 1: Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine


Example 1-1 (Reference Example): Synthesis of (1E,6E)-1-(1H-indol-6-yl)-7-[4-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0070] To 2.5 g of (E)-6-(1H-indol-6-yl)hex-5-ene-2,4-dione were added 44 mL of AcOEt and 1.07 g of boron oxide, and the mixture was stirred at 70°C for 40 minutes. To this, 2.27 g of 4-(2-morpholinoethoxy)benzaldehyde and 7.07 mL of tributyl borate were successively added, and the mixture was stirred at the same temperature for 40 minutes. Then, 0.11 mL of piperidine was added, and the mixture was stirred at 70°C for 1.5 hours and was allowed to cool back to room temperature. To the reaction mixture, 22 mL of a 20% K2CO3 aqueous solution and 20 mL of THF were added and the mixture was stirred at room temperature for 10 minutes. The organic layer was separated, washed with saturated brine, dried over MgSO4, and concentrated. The residue was purified by silica gel column chromatography (CHCl3/acetone or CHCl3/methanol system) to give 1.62 g of the title compound (33% yield).

Example 1-2 (Compound of the invention): Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine



[0071] To 1.5 g of (1E,6E)-1-(1H-indol-6-yl)-7-[4-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione were added 13.5 mL of AcOH and 1.31 mL of hydrazine monohydrate, and the mixture was stirred at 60°C for 3 hours. The reaction mixture was allowed to cool back to room temperature and poured into a stirring mixture of 18.7 g of K2CO3, 95 mL of cold water, 30 mL of AcOEt and 20 mL of THF. The organic layer was separated, washed with saturated brine, dried over MgSO4, and concentrated. The residue was purified by silica gel column chromatography (CHCl3/acetone or CHCl3/methanol system) to give 0.62 g of the title compound (42% yield).

Example 2: Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(1,3-benzodioxol-5-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-methoxyphenoxy]ethyl]morpholine


Example 2-1 (Reference Example): Synthesis of (1E,6E)-1-(1,3-benzodioxol-5-yl)-7-[2-methoxy-4-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0072] The title compound was obtained in the same manner as in Example 1-1.

Example 2-2 (Compound of the invention): Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(1,3-benzodioxol-5-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-methoxyphenoxy]ethyl]morpholine



[0073] The title compound was obtained in the same manner as in Example 1-2.

Example 3: Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(1,3-benzodioxol-5-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine


Example 3-1 (Reference Example): Synthesis of (1E,6E)-1-(1,3-benzodioxol-5-yl)-7-[4-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0074] The title compound was obtained in the same manner as in Example 1-1.

Example 3-2 (Compound of the invention): Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(1,3-benzodioxol-5-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine



[0075] The title compound was obtained in the same manner as in Example 1-2.

Example 4: Synthesis of 6-[(E)-2-[3-[(E)-2-[2-methoxy-4-[(4-methylpiperazin-1-yl)methyl]phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1H-indole


Example 4-1 (Reference Example): Synthesis of 2-methoxy-4-[(4-methylpiperazin-1-yl)methyl]benzoic acid methyl ester



[0076] To 100 mL of ethanol were added 10.0 g of 1-methylpiperazine and 20.2 g of triethylamine. To the mixture cooled with ice, 25 mL of a solution of 24.6 g of 4-(bromomethyl)-2-methoxy-benzoic acid methyl ester in ethanol was added dropwise. The mixture was stirred at room temperature for 18 hours. The reaction mixture was concentrated. To the residue, 75 mL of water and 250 mL of methylene chloride were added, and the organic layer was separated. The obtained organic layer was dried over Na2SO4 and concentrated. The residue was purified by amino-silica gel column chromatography (heptane/AcOEt system) to give 22.4 g of the title compound (85% yield).

Example 4-2 (Reference Example): Synthesis of [2-methoxy-4-[(4-methylpiperazin-1-yl)methyl]phenyl]methanol



[0077] To 200 mL of a suspension of 5.21 g of LAH in THF cooled with ice was added dropwise, under argon flow, 50 mL of a solution of 25.5 g of 2-methoxy-4-[(4-methylpiperazin-1-yl)methyl]benzoic acid methyl ester in THF. The mixture was stirred under ice cooling for 1 hour and further stirred at room temperature for 22 hours. To the reaction mixture cooled with ice, 25 mL of a 10% NaOH water was added dropwise. After 30-minute stirring, the mixture was filtered through Celite. The filtrate was washed with 150 mL of THF and then was concentrated. To the residue, 50 mL of saturated brine and 300 mL of dichloromethane were added, and the organic layer was separated. The organic layer was concentrated. The residue was purified by silica gel column chromatography (CHCl3/methanol system) to give 22.4 g of the title compound (98% yield).

Example 4-3 (Reference Example): Synthesis of 2-methoxy-4-[(4-methylpiperazin-1-yl)methyl]benzaldehyde



[0078] To 220 mL of a solution of 22.2 g of [2-methoxy-4-[(4-methylpiperazin-1-yl)methyl]phenyl]methanol in CHCl3 was added 61.8 g of manganese dioxide, and the mixture was stirred at 55°C for 13 hours. The reaction mixture was allowed to stand to cool. After insoluble matter was filtered off, the mother liquor was concentrated to give 21.8 g of the title compound (99% yield).

Example 4-4 (Reference Example): Synthesis of (1E,6E)-1-(1H-indol-6-yl)-7-[2-methoxy-4-[(4-methylpiperazin-1-yl)methyl]phenyl]hepta-1,6-diene-3,5-dione



[0079] To 200 mL of a solution of 7.56 g of (E)-6-(1H-indol-6-yl)hex-5-ene-2,4-dione in AcOEt was added 3.24 g of boron oxide, and the mixture was stirred at 70°C for 1 hour. To this, 15 mL of a solution of 8.68 g of 2-methoxy-4-[(4-methylpiperazin-1-yl)methyl]benzaldehyde in AcOEt and 18.3 mL of triisopropyl borate were successively added, and the mixture was stirred at the same temperature for 40 minutes. Then, 10 mL of a solution of 0.35 mL of piperidine in AcOEt was added. The mixture was stirred at 70°C for 3 hours and was allowed to cool back to room temperature. To the reaction mixture, 100 mL of a 10% K2CO3 aqueous solution was added, and the mixture was stirred for 1 hour. The organic layer was separated and concentrated. The residue was purified by amino-silica gel column chromatography (heptane/AcOEt system) to give 7.14 g of the title compound (47% yield).

Example 4-5 (Compound of the invention): Synthesis of 6-[(E)-2-[3-[(E)-2-[2-methoxy-4-[(4-methylpiperazin-1-yl)methyl]phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1H-indole



[0080] The title compound was obtained in the same manner as in Example 1-2.

Example 5: Synthesis of 3,5-bis[(E)-2-[4-(2-morpholinoethoxy)phenyl]vinyl]-1H-pyrazole


Example 5-1 (Reference Example): Synthesis of (1E,6E)-1,7-bis[4-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0081] To 2.0 g of pentane-2,4-dione were added 10.0 mL of AcOEt and 0.70 g of boron oxide, and the mixture was stirred at 70°C for 1 hour. To the reaction mixture, 30 mL of a solution of 9.4 g of 4-(2-morpholinoethoxy)benzaldehyde in AcOEt and 10.7 mL of tributyl borate were successively added, and the mixture was stirred at the same temperature for 1 hour. To this, 0.4 mL of 1-butylamine was added, and the mixture was stirred at 70°C for 2.5 hours and was allowed to cool back to room temperature. To the reaction mixture, 50 mL of a 10% K2CO3 aqueous solution and 30 mL of THF were added, and the mixture was stirred at room temperature for 10 minutes. The organic layer was separated, washed with saturated brine, dried over MgSO4, and concentrated. The residue was treated with 100 mL of diethyl ether to give 2.51 g of the title compound as a powder (24% yield).

Example 5-2 (Compound of the invention): Synthesis of 3,5-bis[(E)-2-[4-(2-morpholinoethoxy)phenyl]vinyl]-1H-pyrazole



[0082] The title compound was obtained in the same manner as in Example 1-2.

Example 6: Synthesis of N,N-dimethyl-2-[4-[(E)-2-[3-[(E)-2-[4-(2-morpholinoethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]phenoxy]acetamide


Example 6-1 (Reference Example): Synthesis of (E)-6-[4-(2-morpholinoethoxy)phenyl]hex-5-ene-2,4-dione



[0083] To 30.0 g of (E)-3-[4-(2-morpholinoethoxy)phenyl]-2-propenoic acid was added 600 mL of THF under argon flow. To the mixture cooled with ice, 12.1 g of triethylamine and 15.5 g of IBCF were successively added dropwise. The mixture was stirred at room temperature for 2 hours to prepare an acid anhydride. In a separate container, 200 mL of THF and 16.3 g of acetylacetone were added to 15.5 g of magnesium chloride under argon flow. To the mixture cooled with ice, 17.5 mL of pyridine was added dropwise over 25 minutes. The mixture was stirred at room temperature for 2 hours. To the reaction mixture cooled with ice, the above-prepared acid anhydride cooled with ice was added dropwise over 45 minutes. The mixture was stirred at room temperature overnight. The reaction mixture was added to 2 L of a saturated aqueous ammonium chloride solution and 2 L of CHCl3, and the organic layer was separated. The obtained organic layer was dried over Na2SO4 and concentrated.

[0084] The residue was dissolved in 100 mL of THF. While the mixture was stirred under ice cooling, 40 mL of a 28% ammonia water was added dropwise. The mixture was stirred at the same temperature for 1 hour. The reaction mixture was added to 1 L of CHCl3 and 1 L of saturated brine, and the organic layer was separated. The obtained organic layer was dried over Na2SO4 and concentrated. The residue was purified by silica gel column chromatography (CHCl3/methanol system) to give 26.5 g of the title compound (77% yield).

Example 6-2 (Reference Example): Synthesis of N,N-dimethyl-2-[4-[(1E,6E)-7-[4-(2-morpholinoethoxy)phenyl]-3,5-dioxo-hepta-1,6-dienyl]phenoxy]acetamide



[0085] The title compound was obtained in the same manner as in Example 4-4.

Example 6-3 (compound of the invention): Synthesis of N,N-dimethyl-2-[4-[(E)-2-[3-[(E)-2-[4-(2-morpholinoethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]phenoxy]acetamide



[0086] To 2.70 g of N,N-dimethyl-2-[4-[(1E,6E)-7-[4-(2-morpholinoethoxy)phenyl]-3,5-dioxo-hepta-1,6-dienyl]phenoxy]acetamide were added 2.80 g of hydrazine dihydrochloride and 54 mL of methanol, and the mixture was stirred at 40°C overnight. The reaction mixture was allowed to stand to cool, and poured into 200 mL of a saturated aqueous NaHCO3 solution. To this, 600 mL of AcOEt was added, and the organic layer was separated. The obtained organic layer was washed with saturated brine, dried over Na2SO4, and concentrated. The residue was purified by silica gel column chromatography (CHCl3/methanol system) to give 1.76 g of the title compound (66% yield).

Example 7: Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(3,4-dimethoxyphenyl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine


Example 7-1 (Reference Example): Synthesis of (1E,6E)-1-(3,4-dimethoxyphenyl)-7-[4-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0087] The title compound was obtained in the same manner as in Example 4-4.

Example 7-2 (Compound of the invention): Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(3,4-dimethoxyphenyl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine



[0088] The title compound was obtained in the same manner as in Example 6-3.

Example 8: Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(3H-benzimidazol-5-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine


Example 8-1 (Reference Example): Synthesis of (1E,6E)-1-(3H-benzimidazol-5-yl)-7-[4-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0089] The title compound was obtained in the same manner as in Example 4-4.

Example 8-2 (Compound of the invention): Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(3H-benzimidazol-5-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine



[0090] The title compound was obtained in the same manner as in Example 6-3.

Example 9: Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(1H-pyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 9-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(1H-pyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0091] The title compound was obtained in the same manner as in Example 1-1.

Example 9-2 (Compound of the invention): Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(1H-pyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0092] To 30 mg of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(1H-pyrrol-2-yl)hepta-1,6-diene-3,5-dione were added 1 mL of THF, 3 µL of AcOH and 4 µL of hydrazine monohydrate, and the mixture was stirred at 60°C for 4 hours. To this, 500 µL of AcOH was added and the mixture was stirred at 80°C for 1 hour. To this, 4 µL of hydrazine monohydrate was added and the mixture was stirred at 80°C for 1 hour. The reaction mixture was allowed to cool back to room temperature, and a saturated aqueous NaHCO3 solution was added. After extraction with AcOEt, the organic layer was separated. The obtained organic layer was washed with saturated brine, dried over MgSO4, and concentrated. The residue was purified by PTLC (CHCl3/methanol or AcOEt system) to give 8 mg of the title compound (27% yield).

Example 10: Synthesis of 3-benzyloxy-N,N-diethyl-4-[(E)-2-[5-[(E)-2-(1-methylindol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]aniline


Example 10-1 (Reference Example): Synthesis of (E)-6-(1-methylindol-6-yl)hex-5-ene-2,4-dione



[0093] The title compound was obtained in the same manner as in Example 6-1.

Example 10-2 (Reference Example): Synthesis of (1E,6E)-1-[2-benzyloxy-4-(diethylamino)phenyl]-7-(1-methylindol-6-yl)hepta-1,6-diene-3,5-dione



[0094] The title compound was obtained in the same manner as in Example 1-1.

Example 10-3 (Compound of the invention): Synthesis of 3-benzyloxy-N,N-diethyl-4-[(E)-2-[5-[(E)-2-(1-methylindol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]aniline



[0095] The title compound was obtained in the same manner as in Example 9-2.

Example 11: Synthesis of N,N-diethyl-4-[(E)-2-[5-[(E)-2-(1-methylindol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-(2-morpholinoethoxy)aniline


Example 11-1 (Reference Example): Synthesis of (1E,6E)-1-[4-(diethylamino)-2-(2-morpholinoethoxy)phenyl]-7-(1-methylindol-6-yl)hepta-1,6-diene-3,5-dione



[0096] The title compound was obtained in the same manner as in Example 1-1.

Example 11-2 (Compound of the invention): Synthesis of N,N-diethyl-4-[(E)-2-[5-[(E)-2-(1-methylindol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-(2-morpholinoethoxy)aniline



[0097] The title compound was obtained in the same manner as in Example 9-2.

Example 12: Synthesis of 2-[(E)-2-[5-[(E)-2-(1-methylindol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-5-(2-pyridylmethoxy)phenol


Example 12-1 (Reference Example): Synthesis of (1E,6E)-1-[2-hydroxy-4-(2-pyridylmethoxy)phenyl]-7-(1-methylindol-6-yl)hepta-1,6-diene-3,5-dione



[0098] The title compound was obtained in the same manner as in Example 1-1.

Example 12-2 (Compound of the invention): Synthesis of 2-[(E)-2-[5-[(E)-2-(1-methylindol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-5-(2-pyridylmethoxy)phenol



[0099] The title compound was obtained in the same manner as in Example 9-2.

Example 13: Synthesis of 4-[2-[2-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-5-(2-pyridylmethoxy)phenoxy]ethyl]morpholine


Example 13-1 (Reference Example): Synthesis of 2-(2-morpholinoethoxy)-4-(2-pyridylmethoxy)benzaldehyde



[0100] To 1.0 g of 2-hydroxy-4-(2-pyridylmethoxy)benzaldehyde were successively added 30 mL of acetonitrile, 4.26 g of cesium carbonate and 0.85 g of 4-(2-chloroethyl)morpholine hydrochloride. The mixture was stirred at 90°C for 2 hours. To the reaction mixture, 50 mL of water and 150 mL of AcOEt were added, and the organic layer was separated. The obtained organic layer was dried over Na2SO4 and concentrated to give 1.56 g of the title compound in quantitative yield.

Example 13-2 (Reference Example): (1E,6E)-1-(1H-indol-6-yl)-7-[2-(2-morpholinoethoxy)-4-(2-pyridylmethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0101] The title compound was obtained in the same manner as in Example 1-1.

Example 13-3 (Compound of the invention: Synthesis of 4-[2-[2-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-5-(2-pyridylmethoxy)phenoxy]ethyl]morpholine



[0102] The title compound was obtained in the same manner as in Example 9-2.

Example 14: Synthesis of 4-[2-[3-methoxy-4-[(E)-2-[5-[(E)-2-(1-methylindol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine


Example 14-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-morpholinoethoxy)phenyl]-7-(1-methylindol-6-yl)hepta-1,6-diene-3,5-dione



[0103] The title compound was obtained in the same manner as in Example 1-1.

Example 14-2 (Compound of the invention): Synthesis of 4-[2-[3-methoxy-4-[(E)-2-[5-[(E)-2-(1-methylindol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine



[0104] The title compound was obtained in the same manner as in Example 1-2.

Example 15: Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(1-methylindol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine


Example 15-1 (Reference Example): Synthesis of (1E,6E)-1-(1-methylindol-6-yl)-7-[4-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0105] The title compound was obtained in the same manner as in Example 1-1.

Example 15-2 (Compound of the invention): Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(1-methylindol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine



[0106] The title compound was obtained in the same manner as in Example 1-2.

Example 16: Synthesis of 6-[(E)-2-[3-[(E)-2-[2-fluoro-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1-methylindole


Example 16-1 (Reference Example): Synthesis of (1E,6E)-1-[2-fluoro-4-(2-pyridylmethoxy)phenyl]-7-(1-methylindol-6-yl)hepta-1,6-diene-3,5-dione



[0107] The title compound was obtained in the same manner as in Example 1-1.

Example 16-2 (Compound of the invention): Synthesis of 6-[(E)-2-[3-[(E)-2-[2-fluoro-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1-methylindole



[0108] The title compound was obtained in the same manner as in Example 1-2.

Example 17: Synthesis of 1-methyl-6-[(E)-2-[3-[(E)-2-[4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]indole


Example 17-1 (Reference Example): Synthesis of (1E,6E)-1-(1-methylindol-6-yl)-7-[4-(2-pyridylmethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0109] The title compound was obtained in the same manner as in Example 1-1.

Example 17-2)Compound of the invention): Synthesis of 1-methyl-6-[(E)-2-[3-[(E)-2-[4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]indole



[0110] The title compound was obtained in the same manner as in Example 1-2.

Example 18: Synthesis of 1-methyl-6-[(E)-2-[3-[(E)-2-[4-(4-methylpiperazin-1-yl)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]indole


Example 18-1 (Reference Example): Synthesis of (1E,6E)-1-(1-methylindol-6-yl)-7-[4-(4-methylpiperazin-1-yl)phenyl]hepta-1,6-diene-3,5-dione



[0111] The title compound was obtained in the same manner as in Example 1-1.

Example 18-2 (Compound of the invention): Synthesis of 1-methyl-6-[(E)-2-[3-[(E)-2-[4-(4-methylpiperazin-1-yl)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]indole



[0112] The title compound was obtained in the same manner as in Example 1-2.

Example 19: Synthesis of 2-[[3-fluoro-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 19-1 (Reference Example): Synthesis of (E)-3-[2-fluoro-4-(2-pyridylmethoxy)phenyl]-2-propenoic acid



[0113] To 1.8 g of malonic acid were added 18 mL of pyridine, 2.0 g of 2-fluoro-4-(2-pyridylmethoxy)benzaldehyde and 0.21 mL of piperidine. The mixture was stirred at 75°C for 1.5 hours and further stirred at 95°C for 1 hour. The reaction mixture was cooled with ice, water was added thereto, and 10% hydrochloric acid was added to adjust the pH to 4. The resulting precipitate was separated by filtration to give 2.0 g of the title compound (85% yield).

Example 19-2 (Reference Example): Synthesis of (E)-6-[2-fluoro-4-(2-pyridylmethoxy)phenyl]hex-5-ene-2,4-dione



[0114] The title compound was obtained in the same manner as in Example 6-1.

Example 19-3 (Reference Example): Synthesis of (1E,6E)-1-[2-fluoro-4-(2-pyridylmethoxy)phenyl]-7-(1-methylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0115] The title compound was obtained in the same manner as in Example 1-1.

Example 19-4 (Compound of the invention): Synthesis of 2-[[3-fluoro-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0116] The title compound was obtained in the same manner as in Example 1-2.

Example 20: Synthesis of 2-[[4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 20-1 (Reference Example): Synthesis of (E)-6-[4-(2-pyridylmethoxy)phenyl]hex-5-ene-2,4-dione



[0117] The title compound was obtained in the same manner as in Example 6-1.

Example 20-2 (Reference Example): Synthesis of (1E,6E)-1-(1-methylpyrrol-2-yl)-7-[4-(2-pyridylmethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0118] The title compound was obtained in the same manner as in Example 1-1.

Example 20-3 (Compound of the invention): Synthesis of 2-[[4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0119] The title compound was obtained in the same manner as in Example 1-2.

Example 21: Synthesis of 4-[2-[3-methoxy-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine


Example 21-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-morpholinoethoxy)phenyl]-7-(1-methylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0120] The title compound was obtained in the same manner as in Example 1-1.

Example 21-2 (Compound of the invention): Synthesis of 4-[2-[3-methoxy-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine



[0121] The title compound was obtained in the same manner as in Example 1-2.

Example 22: Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 22-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(1-methylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0122] The title compound was obtained in the same manner as in Example 1-1.

Example 22-2 (Compound of the invention): Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0123] The title compound was obtained in the same manner as in Example 1-2.

Example 23: Synthesis of 2-[[4-[(E)-2-[5-[(E)-2-(1,5-dimethylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-methoxyphenoxy]methyl]pyridine


Example 23-1 (Reference Example): Synthesis of (1E,6E)-1-(1,5-dimethylpyrrol-2-yl)-7-[2-methoxy-4-(2-pyridylmethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0124] The title compound was obtained in the same manner as in Example 1-1.

Example 23-2 (Compound of the invention): Synthesis of 2-[[4-[(E)-2-[5-[(E)-2-(1,5-dimethylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-methoxyphenoxy]methyl]pyridine



[0125] The title compound was obtained in the same manner as in Example 1-2.

Example 24: Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(1,3,5-trimethylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 24-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(1,3,5-trimethylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0126] The title compound was obtained in the same manner as in Example 1-1.

Example 24-2 (Compound of the invention): Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(1,3,5-trimethylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0127] The title compound was obtained in the same manner as in Example 1-2.

Example 25: Synthesis of 2-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-6-methylpyridine


Example 25-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(6-methyl-2-pyridyl)hepta-1,6-diene-3,5-dione



[0128] The title compound was obtained in the same manner as in Example 1-1.

Example 25-2 (Compound of the invention): Synthesis of 2-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-6-methylpyridine



[0129] The title compound was obtained in the same manner as in Example 1-2.

Example 26: Synthesis of 3-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-2-methylpyridine


Example 26-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(2-methyl-3-pyridyl)hepta-1,6-diene-3,5-dione



[0130] The title compound was obtained in the same manner as in Example 1-1.

Example 26-2 (Compound of the invention): Synthesis of 3-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-2-methylpyridine



[0131] The title compound was obtained in the same manner as in Example 1-2.

Example 27: Synthesis of 1-methyl-4-[4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]piperazine


Example 27-1 (Reference Example): Synthesis of (E)-6-(1-methylpyrrol-2-yl)hex-5-ene-2,4-dione



[0132] The title compound was obtained in the same manner as in Example 6-1.

Example 27-2 (Reference Example): Synthesis of (1E,6E)-1-[4-(4-methylpiperazin-1-yl)phenyl]-7-(1-methylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0133] The title compound was obtained in the same manner as in Example 1-1.

Example 27-3 (Compound of the invention): Synthesis of 1-methyl-4-[4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]piperazine



[0134] The title compound was obtained in the same manner as in Example 1-2.

Example 28: Synthesis of 1-methyl-4-[4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]-1,4-diazepane


Example 28-1 (Reference Example): Synthesis of (1E,6E)-1-[4-(4-methyl-1,4-diazepan-1-yl)phenyl]-7-(1-methylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0135] The title compound was obtained in the same manner as in Example 1-1.

Example 28-2 (Compound of the invention): Synthesis of 1-methyl-4-[4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]-1,4-diazepane



[0136] The title compound was obtained in the same manner as in Example 1-2.

Example 29: Synthesis of 3-[(E)-2-(4-isopropoxy-2-methoxyphenyl)vinyl]-5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazole


Example 29-1 (Reference Example): Synthesis of (1E,6E)-1-(4-isopropoxy-2-methoxyphenyl)-7-(1-methylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0137] The title compound was obtained in the same manner as in Example 1-1.

Example 29-2 (Compound of the invention): Synthesis of 3-[(E)-2-(4-isopropoxy-2-methoxyphenyl)vinyl]-5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazole



[0138] The title compound was obtained in the same manner as in Example 1-2.

Example 30: Synthesis of 1-methyl-4-[2-[4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]ethyl]piperazine


Example 30-1 (Reference Example): Synthesis of 4-[2-(4-methylpiperazin-1-yl)ethyl]benzaldehyde



[0139] To 87 mL of a solution of 8.7 g of 1-[2-(4-bromophenyl)ethyl]-4-methylpiperazine in THF cooled to -70°C was added dropwise 21.1 mL of a solution of n-butyllithium (1.6 mol/L) in hexane, and the mixture was stirred at the same temperature for 30 minutes. While the reaction mixture was stirred at the same temperature, 3.6 mL of N,N-dimethylformamide was added dropwise. The mixture was stirred at the same temperature for 30 minutes. The cooling bath was removed, and the mixture was allowed to warm to -10°C, poured into a mixture of 12 mL of 1 N hydrochloric acid and 100 mL of cold water, and subjected to extraction with CHCl3. The obtained extract was dried over MgSO4 and concentrated to give 7.04 g of the title compound (98%).

Example 30-2 (Reference Example): Synthesis of (1E,6E)-1-[4-[2-(4-methylpiperazin-1-yl)ethyl]phenyl]-7-(1-methylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0140] The title compound was obtained in the same manner as in Example 1-1.

Example 30-3 (Compound of the invention): Synthesis of 1-methyl-4-[2-[4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]ethyl]piperazine



[0141] The title compound was obtained in the same manner as in Example 1-2.

Example 31: Synthesis of 2-[2-[3-methoxy-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]pyridine


Example 31-1 (Reference Example): Synthesis of 2-methoxy-4-[2-(2-pyridyl)ethoxy]benzaldehyde



[0142] To a stirring ice-cooled mixture of 5.0 g of 4-hydroxy-2-methoxybenzaldehyde, 4.5 g of 2-(2-pyridyl)ethanol and 10.3 g of triphenylphosphine was added dropwise 15.8 g of a 40% solution of diethyl azodicarboxylate in toluene. The mixture was stirred at room temperature for 5 hours. The reaction mixture was concentrated, purified by silica gel column chromatography (hexane/acetone system) and further purified by silica gel column chromatography (hexane/AcOEt system) to give 5.84 g of the title compound (69% yield).

Example 31-2 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-[2-(2-pyridyl)ethoxy]phenyl]-7-(1-methylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0143] The title compound was obtained in the same manner as in Example 1-1.

Example 31-3 (Compound of the invention): Synthesis of 2-[2-[3-methoxy-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]pyridine



[0144] The title compound was obtained in the same manner as in Example 1-2.

Example 32: Synthesis of 2-[[3-methyl-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 32-1 (Reference Example): Synthesis of 2-methyl-4-(2-pyridylmethoxy)benzoic acid methyl ester



[0145] To 0.85 g of 4-hydroxy-2-methyl benzoic acid methyl ester were added 12 mL of acetonitrile, 2.12 g of K2CO3 and 0.84 g of 2-(chloromethyl)pyridine hydrochloride, and the mixture was stirred at 90°C for 4 hours. The reaction mixture was allowed to cool back to room temperature and neutralized by the addition of 5% hydrochloric acid. To this, 150 mL of AcOEt was added, and the organic layer was separated. The obtained organic layer was washed with saturated brine, dried over MgSO4, and concentrated. The residue was purified by silica gel column chromatography (hexane/AcOEt system) to give 1.18 g of the title compound (89% yield).

Example 32-2 (Reference Example): Synthesis of [2-methyl-4-(2-pyridylmethoxy)phenyl]methanol



[0146] To 1.0 g of 2-methyl-4-(2-pyridylmethoxy)benzoic acid methyl ester was added 8 mL of toluene under argon atmosphere. To the mixture cooled to -78°C, 8.7 mL of a solution of diisobutylaluminium hydride (1.0 mol/L) in toluene was added dropwise. The mixture was stirred at the same temperature for 6 hours and further stirred at -35°C for 1 hour. To the reaction mixture, a saturated aqueous Rochelle salt solution was added, and the mixture was stirred at room temperature for 30 minutes. To this, 150 mL of diethyl ether was added, and the organic layer was separated. The obtained organic layer was dried over Na2SO4 and concentrated to give 0.83 g of the title compound (93% yield).

Example 32-3 (Reference Example): Synthesis of 2-methyl-4-(2-pyridylmethoxy)benzaldehyde



[0147] To 0.72 g of [2-methyl-4-(2-pyridylmethoxy)phenyl]methanol were added 30 mL of CHCl3 and 7.2 g of manganese dioxide, and the mixture was stirred at room temperature for 2 hours. Insoluble matter was filtered off from the reaction mixture, and the mother liquor was concentrated to give 0.70 g of the title compound (99% yield).

Example 32-4 (Reference Example): Synthesis of (1E,6E)-1-[2-methyl-4-(2-pyridylmethoxy)phenyl]-7-(1-methylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0148] The title compound was obtained in the same manner as in Example 1-1.

Example 32-5 (Compound of the invention): Synthesis of 2-[[3-methyl-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0149] The title compound was obtained in the same manner as in Example 1-2.

Example 33: Synthesis of 2-[2-[3-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]pyridine


Example 33-1 (Reference Example): Synthesis of (1E,6E)-1-(1-methylpyrrol-2-yl)-7-[3-[2-(2-pyridyl)ethoxy]phenyl]hepta-1,6-diene-3,5-dione



[0150] The title compound was obtained in the same manner as in Example 1-1.

Example 33-2 (Compound of the invention): Synthesis of 2-[2-[3-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]pyridine



[0151] The title compound was obtained in the same manner as in Example 1-2.

Example 34: Synthesis of 2-[[3,5-dimethoxy-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 34-1 (Reference Example): Synthesis of 2,6-dimethoxy-4-(2-pyridylmethoxy)benzaldehyde



[0152] The title compound was obtained in the same manner as in Example 32-1.

Example 34-2 (Reference Example): Synthesis of (1E,6E)-1-[2,6-dimethoxy-4-(2-pyridylmethoxy)phenyl]-7-(1-methylpyrrol-2-yl)hepta-1,6-diene-3,5-dione



[0153] The title compound was obtained in the same manner as in Example 1-1.

Example 34-3 (Compound of the invention): Synthesis of 2-[[3,5-dimethoxy-4-[(E)-2-[5-[(E)-2-(1-methylpyrrol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0154] The title compound was obtained in the same manner as in Example 1-2.

Example 35: Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(1-methylimidazol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 35-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(1-methylimidazol-2-yl)hepta-1,6-diene-3,5-dione



[0155] The title compound was obtained in the same manner as in Example 1-1.

Example 35-2 (Compound of the invention): Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(1-methylimidazol-2-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0156] The title compound was obtained in the same manner as in Example 1-2.

Example 36: Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(3-methylimidazol-4-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 36-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(3-methylimidazol-4-yl)hepta-1,6-diene-3,5-dione



[0157] The title compound was obtained in the same manner as in Example 1-1.

Example 36-2 (Compound of the invention): Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(3-methylimidazol-4-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0158] The title compound was obtained in the same manner as in Example 1-2.

Example 37: Synthesis of 2-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]pyridine


Example 37-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(2-pyridyl)hepta-1,6-diene-3,5-dione



[0159] The title compound was obtained in the same manner as in Example 1-1.

Example 37-2 (Compound of the invention): Synthesis of 2-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]pyridine



[0160] The title compound was obtained in the same manner as in Example 1-2.

Example 38: Synthesis of 7-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]quinoline


Example 38-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(7-quinolyl)hepta-1,6-diene-3,5-dione



[0161] The title compound was obtained in the same manner as in Example 1-1.

Example 38-2 (Compound of the invention): Synthesis of 7-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]quinoline



[0162] The title compound was obtained in the same manner as in Example 1-2.

Example 39: Synthesis of 2-[[4-[(E)-2-[5-[(E)-2-(2-furyl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-methoxyphenoxy]methyl]pyridine


Example 39-1 (Reference Example): Synthesis of (1E,6E)-1-(2-furyl)-7-[2-methoxy-4-(2-pyridylmethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0163] The title compound was obtained in the same manner as in Example 1-1.

Example 39-2 (Compound of the invention): Synthesis of 2-[[4-[(E)-2-[5-[(E)-2-(2-furyl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-methoxyphenoxy]methyl]pyridine



[0164] The title compound was obtained in the same manner as in Example 1-2.

Example 40: Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(5-methyl-2-furyl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 40-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(5-methyl-2-furyl)hepta-1,6-diene-3,5-dione



[0165] The title compound was obtained in the same manner as in Example 1-1.

Example 40-2 (Compound of the invention): Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(5-methyl-2-furyl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0166] The title compound was obtained in the same manner as in Example 1-2.

Example 41: Synthesis of 2-[[4-[(E)-2-[5-[(E)-2-(4,5-dimethyl-2-furyl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-methoxyphenoxy]methyl]pyridine


Example 41-1 (Reference Example): Synthesis of (1E,6E)-1-(4,5-dimethyl-2-furyl)-7-[2-methoxy-4-(2-pyridylmethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0167] The title compound was obtained in the same manner as in Example 1-1.

Example 41-2 (Compound of the invention): Synthesis of 2-[[4-[(E)-2-[5-[(E)-2-(4,5-dimethyl-2-furyl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-methoxyphenoxy]methyl]pyridine



[0168] The title compound was obtained in the same manner as in Example 1-2.

Example 42: Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(2-methylpyrazol-3-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine


Example 42-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(2-methylpyrazol-3-yl)hepta-1,6-diene-3,5-dione



[0169] The title compound was obtained in the same manner as in Example 1-1.

Example 42-2 (Compound of the invention): Synthesis of 2-[[3-methoxy-4-[(E)-2-[5-[(E)-2-(2-methylpyrazol-3-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]methyl]pyridine



[0170] The title compound was obtained in the same manner as in Example 1-2.

Example 43: Synthesis of 1-methyl-6-[(E)-2-[3-[(E)-2-[4-(4-methyl-1,4-diazepan-1-yl)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]indole


Example 43-1 (Reference Example): Synthesis of (1E,6E)-1-[4-(4-methyl-1,4-diazepan-1-yl)phenyl]-7-(1-methylindol-6-yl)hepta-1,6-diene-3,5-dione



[0171] The title compound was obtained in the same manner as in Example 1-1.

Example 43-2 (Compound of the invention): Synthesis of 1-methyl-6-[(E)-2-[3-[(E)-2-[4-(4-methyl-1,4-diazepan-1-yl)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]indole



[0172] The title compound was obtained in the same manner as in Example 1-2.

Example 44: Synthesis of 6-[(E)-2-[3-[(E)-2-[2-methoxy-4-[2-(2-pyridyl)ethoxy]phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1-methylindole


Example 44-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-[2-(2-pyridyl)ethoxy]phenyl]-7-(1-methylindol-6-yl)hepta-1,6-diene-3,5-dione



[0173] The title compound was obtained in the same manner as in Example 1-1.

Example 44-2 (Compound of the invention): Synthesis of 6-[(E)-2-[3-[(E)-2-[2-methoxy-4-[2-(2-pyridyl)ethoxy]phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1-methylindole



[0174] The title compound was obtained in the same manner as in Example 1-2.

Example 45: Synthesis of 6-[(E)-2-[3-[(E)-2-[2,6-dimethoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1-methylindole


Example 45-1 (Reference Example): Synthesis of (1E,6E)-1-[2,6-dimethoxy-4-(2-pyridylmethoxy)phenyl]-7-(1-methylindol-6-yl)hepta-1,6-diene-3,5-dione



[0175] The title compound was obtained in the same manner as in Example 1-1.

Example 45-2 (Compound of the invention): Synthesis of 6-[(E)-2-[3-[(E)-2-[2,6-dimethoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1-methylindole



[0176] The title compound was obtained in the same manner as in Example 1-2.

Example 46: Synthesis of 6-[(E)-2-[3-[(E)-2-[3-fluoro-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1-methylindole


Example 46-1 (Reference Example): Synthesis of (1E,6E)-1-[3-fluoro-4-(2-pyridylmethoxy)phenyl]-7-(1-methylindol-6-yl)hepta-1,6-diene-3,5-dione



[0177] The title compound was obtained in the same manner as in Example 1-1.

Example 46-2 (Compound of the invention): Synthesis of 6-[(E)-2-[3-[(E)-2-[3-fluoro-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1-methylindole



[0178] The title compound was obtained in the same manner as in Example 1-2.

Example 47: Synthesis of 4-[[4-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]methyl]morpholine


Example 47-1 (Reference Example): Synthesis of (1E,6E)-1-(1H-indol-6-yl)-7-[4-(morpholinomethyl)phenyl]hepta-1,6-diene-3,5-dione



[0179] The title compound was obtained in the same manner as in Example 1-1.

Example 47-2 (Compound of the invention): Synthesis of 4-[[4-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]methyl]morpholine



[0180] The title compound was obtained in the same manner as in Example 1-2.

Example 48: Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]ethyl]morpholine


Example 48-1 (Reference Example): Synthesis of (1E,6E)-1-(1H-indol-6-yl)-7-[4-(2-morpholinoethyl)phenyl]hepta-1,6-diene-3,5-dione



[0181] The title compound was obtained in the same manner as in Example 1-1.

Example 48-2 (Compound of the invention): Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]ethyl]morpholine



[0182] The title compound was obtained in the same manner as in Example 1-2.

Example 49: Synthesis of 4-[3-[4-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]propyl]morpholine


Example 49-1 (Reference Example): Synthesis of (1E,6E)-1-(1H-indol-6-yl)-7-[4-(3-morpholinopropyl)phenyl]hepta-1,6-diene-3,5-dione



[0183] The title compound was obtained in the same manner as in Example 1-1.

Example 49-2 (Compound of the invention): Synthesis of 4-[3-[4-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenyl]propyl]morpholine



[0184] The title compound was obtained in the same manner as in Example 1-2.

Example 50: Synthesis of 6-[(E)-2-[3-[(E)-2-[4-[(4-methylpiperazin-1-yl)methyl]phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1H-indole


Example 50-1 (Reference Example): Synthesis of (1E,6E)-1-(1H-indol-6-yl)-7-[4-[(4-methylpiperazin-1-yl)methyl]phenyl]hepta-1,6-diene-3,5-dione



[0185] The title compound was obtained in the same manner as in Example 1-1.

Example 50-2 (Compound of the invention): Synthesis of 6-[(E)-2-[3-[(E)-2-[4-[(4-methylpiperazin-1-yl)methyl]phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1H-indole



[0186] The title compound was obtained in the same manner as in Example 1-2.

Example 51: Synthesis of 6-[(E)-2-[3-[(E)-2-[4-[2-(4-methylpiperazin-1-yl)ethyl]phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1H-indole


Example 51-1 (Reference Example): Synthesis of (1E,6E)-1-(1H-indol-6-yl)-7-[4-[2-(4-methylpiperazin-1-yl)ethyl]phenyl]hepta-1,6-diene-3,5-dione



[0187] The title compound was obtained in the same manner as in Example 1-1.

Example 51-2 (Compound of the invention): Synthesis of 6-[(E)-2-[3-[(E)-2-[4-[2-(4-methylpiperazin-1-yl)ethyl]phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1H-indole



[0188] The title compound was obtained in the same manner as in Example 1-2.

Example 52: Synthesis of 4-[2-[2-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine


Example 52-1 (Reference Example): Synthesis of (1E,6E)-1-(1H-indol-6-yl)-7-[2-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0189] The title compound was obtained in the same manner as in Example 1-1.

Example 52-2 (Compound of the invention): Synthesis of 4-[2-[2-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine



[0190] The title compound was obtained in the same manner as in Example 1-2.

Example 53: Synthesis of 4-[2-[3-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine


Example 53-1 (Reference Example): Synthesis of (1E,6E)-1-(1H-indol-6-yl)-7-[3-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0191] The title compound was obtained in the same manner as in Example 1-1.

Example 53-2 (Compound of the invention): Synthesis of 4-[2-[3-[(E)-2-[5-[(E)-2-(1H-indol-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]phenoxy]ethyl]morpholine



[0192] The title compound was obtained in the same manner as in Example 1-2.

Example 54: Synthesis of 6-[(E)-2-[3-[(E)-2-[2-methoxy-4-[2-(2-pyridyl)ethoxy]phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1H-indole


Example 54-1 (Reference Example): Synthesis of (1E,6E)-1-(1H-indol-6-yl)-7-[2-methoxy-4-[2-(2-pyridyl)ethoxy]phenyl]hepta-1,6-diene-3,5-dione



[0193] The title compound was obtained in the same manner as in Example 1-1.

Example 54-2 (Compound of the invention): Synthesis of 6-[(E)-2-[3-[(E)-2-[2-methoxy-4-[2-(2-pyridyl)ethoxy]phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1H-indole



[0194] The title compound was obtained in the same manner as in Example 1-2.

Example 55: Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(2,3-dihydro-1,4-benzodioxin-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-methoxyphenoxy]ethyl]morpholine


Example 55-1 (Reference Example): Synthesis of (E)-6-(2,3-dihydro-1,4-benzodioxin-6-yl)hex-5-ene-2,4-dione



[0195] The title compound was obtained in the same manner as in Example 6-1.

Example 55-2 (Reference Example): Synthesis of (1E,6E)-1-(2,3-dihydro-1,4-benzodioxin-6-yl)-7-[2-methoxy-4-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0196] The title compound was obtained in the same manner as in Example 1-1.

Example 55-3 (Compound of the invention): Synthesis of 4-[2-[4-[(E)-2-[5-[(E)-2-(2,3-dihydro-1,4-benzodioxin-6-yl)vinyl]-1H-pyrazol-3-yl]vinyl]-3-methoxyphenoxy]ethyl]morpholine



[0197] The title compound was obtained in the same manner as in Example 1-2.

Example 56: Synthesis of 4-[2-[3-methoxy-4-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-morpholinoethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]phenoxy]ethyl]morpholine


Example 56-1 (Reference Example): Synthesis of (1E,6E)-1,7-bis[2-methoxy-4-(2-morpholinoethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0198] The title compound was obtained in the same manner as in Example 5-1.

Example 56-2 (Compound of the invention): Synthesis of 4-[2-[3-methoxy-4-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-morpholinoethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]phenoxy]ethyl]morpholine



[0199] The title compound was obtained in the same manner as in Example 1-2.

Example 57: Synthesis of 1-methyl-2-[(E)-2-[3-[(E)-2-[4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]indole


Example 57-1 (Reference Example): Synthesis of (1E,6E)-1-(1-methylindol-2-yl)-7-[4-(2-pyridylmethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0200] The title compound was obtained in the same manner as in Example 1-1.

Example 57-2 (Compound of the invention): Synthesis of 1-methyl-2-[(E)-2-[3-[(E)-2-[4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]indole



[0201] The title compound was obtained in the same manner as in Example 1-2.

Example 58: Synthesis of 5-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1-methylindole


Example 58-1 (Reference Example): Synthesis of (1E,6E)-1-[2-methoxy-4-(2-pyridylmethoxy)phenyl]-7-(1-methylindol-5-yl)hepta-1,6-diene-3,5-dione



[0202] The title compound was obtained in the same manner as in Example 1-1.

Example 58-2 (Compound of the invention): Synthesis of 5-[(E)-2-[3-[(E)-2-[2-methoxy-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]-1-methylindole



[0203] The title compound was obtained in the same manner as in Example 1-2.

Example 59: Synthesis of 1-methyl-6-[(E)-2-[3-[(E)-2-[2-methyl-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]indole


Example 59-1 (Reference Example): Synthesis of (1E,6E)-1-(1-methylindol-6-yl)-7-[2-methyl-4-(2-pyridylmethoxy)phenyl]hepta-1,6-diene-3,5-dione



[0204] The title compound was obtained in the same manner as in Example 1-1.

Example 59-2 (Compound of the invention): Synthesis of 1-methyl-6-[(E)-2-[3-[(E)-2-[2-methyl-4-(2-pyridylmethoxy)phenyl]vinyl]-1H-pyrazol-5-yl]vinyl]indole



[0205] The title compound was obtained in the same manner as in Example 1-2.

[0206] The structural formulas and analytical data of the compounds obtained in the above Examples are shown in the tables below.

[0207] Ex.: example; St.: chemical structure; Dat.: analytical data
Table 2 (Compounds 1-2 and 2-2 are compounds of the invention; Compounds 1-1 and 2-1 are Reference Examples)
Ex.St.
Dat.
1-1

1H NMR (δ, CDCl3): 2.56-2.61 (m, 4H), 2.82 (t, 2H, J=5.7 Hz), 3.72-3.76 (m, 4H), 4.15 (t, 2H, J=5.7 Hz), 5.81 (s, 1H), 6.51 (d, 1H, J=16.0 Hz), 6.56-6.58 (m, 1H), 6.64 (d, 1H, J=16.0 Hz), 6.92 (d, 2H, J=8.8 Hz), 7.28-7.30 (m, 1H), 7.40 (dd, 1H, J=1.3Hz, 8.2 Hz), 7.50 (d, 2H, J=8.8 Hz), 7.56 (s, 1H), 7.62 (d, 1H, J=16.0 Hz), 7.63 (d, 1H, J=8.2 Hz), 7.79 (d, 1H, J=16.0 Hz), 8.30 (s, 1H), 16.07 (brs, 1H) MS (EI) m/z 444 (M+).
1-2

1H NMR (δ, CDCl3): 2.55-2.60 (m, 4H), 2.80 (t, 2H, J=5.7 Hz), 3.72-3.75 (m, 4H), 4.11 (t, 2H, J=5.7 Hz), 6.52-6.54 (m, 1H), 6.60 (s, 1H), 6.87 (d, 2H, J=8.7 Hz), 6.92 (d, 1H, J=16.4 Hz), 7.01-7.06 (m, 2H), 7.18-7.20 (m, 1H), 7.19 (d, 1H, J=16.4 Hz), 7.31 (dd, 1H, J=1.4Hz, 8.3 Hz), 7.40 (d, 2H, J=8.7 Hz), 7.43-7.45 (m, 1H), 7.60 (d, 1H, J=8.3 Hz), 8.13 (s, 1H), 9.32-11.42 (br, 1H) MS (EI) m/z 440 (M+).
2-1

MS (EI) m/z 479 (M+)
2-2

1H NMR (δ, CDCl3): 2.61-2.74 (m, 4H), 2.84-2.93 (m, 2H), 3.76-3.83 (m, 4H), 3.87 (s, 3H), 4.16-4.22 (m, 2H), 5.97 (s, 2H) ,6.48-6.52 (m, 2H), 6.58 (s, 1H), 6.79 (d, 1H J=7.8 Hz), 6.88 (d, 1H, J=16.5 Hz), 6.91-6.97 (m, 2H), 7.00 (d, 1H, J=16.5 Hz), 7.03 (d, 1H, J=1.4 Hz), 7.29 (d, 1H, J=16.5 Hz), 7.43 (d, 1H, J=8.2 Hz) MS (EI) m/z 475 (M+).
Table 3 (Compound 3-2 is a compound of the invention; Compound 3-1 is a Reference Example)
3-1

MS (EI) m/z 449 (M+)
3-2

1H NMR (δ, CDCl3): 2.56-2.60 (m, 4H), 2.81 (t, 2H, J=5.7 Hz), 3.74 (m, 4H), 4.14 (t, 2H, J=5.7 Hz), 5.98 (s, 2H) ,6.57 (s, 1H), 6.79 (d, 1H, J=7.8 Hz), 6.84-6.94 (m, 5H), 6.97-7.04 (m, 3H), 7.40-7.43 (m, 2H), 9.48-11.04 (br, 1H) MS (EI) m/z 445 (M+).
Table 4 (Compound 4-5 is a compound of the invention; Compounds 4-1 to 4-4 are Reference Examples)
4-1

1H NMR (δ, CDCl3): 2.29 (s, 3H), 2.47 (brs, 8H), 3.52 (s, 2H), 3.88 (s, 3H), 3.91 (s, 3H), 6.94 (d, 1H, J=7.9 Hz), 7.00 (s, 1H), 7.74 (d, 1H, J=7.9 Hz)
4-2

1H NMR (δ, CDCl3): 2.28 (s, 3H), 2.47 (brs, 8H), 3.49 (s, 2H), 3.87 (s, 3H), 4.66 (s, 2H), 6.86-6.92 (m, 2H), 7.20 (d, 1H, J=7.7 Hz)
4-3

1H NMR (δ, CDCl3): 2.30 (s, 3H), 2.48 (brs, 8H), 3.53 (s, 2H), 3.94 (s, 3H), 6.97-7.02 (m, 2H), 7.77 (d, 1H, J=7.7 Hz), 10.42 (s, 1H)
4-4

1H NMR (δ, DMSO-d6): 2.16 (s, 3H), 2.37 (brs, 8H), 3.48 (s, 2H), 3.89 (s, 3H), 6.14 (s, 1H), 6.47-6.51 (m, 1H), 6.84-6.99 (m, 3H), 7.02 (s, 1H), 7.43 (d, 1H, J=8.3 Hz), 7.48-7.51 (m, 1H), 7.60 (d, 1H, J=8.3 Hz), 7.66-7.74 (m, 2H), 7.79 (d, 1H, J=15.8 Hz), 7.85 (d, 1H, J=15.9 Hz), 11.41 (brs, 1H) MS (ESI) m/z 459 (M+2H)+, 915 (2M+H)+.
4-5

1H NMR (δ, CDCl3): 2.31 (s, 3H), 2.50 (brs, 8H), 3.51 (s, 2H), 3.91 (s, 3H), 6.54-6.56 (m, 1H), 6.67 (s, 1H), 6.91-6.94 (m, 2H), 7.05 (d, 1H, J=16.6 Hz), 7.07 (d, 1H, J=16.6 Hz), 7.18-7.28 (m, 2H), 7.34 (dd, 1H, J=1.5Hz, 8.5 Hz), 7.38 (d, 1H, J=16.6 Hz), 7.47-7.50 (m, 2H), 7.62 (d, 1H, J=8.0 Hz), 8.21 (brs, 1H)
Table 5 (Compounds 5-2 and 6-3 are compounds of the invention; Compounds 5-1, 6-1 and 6-2 are Reference Examples)
5-1

1H NMR (δ, CDCl3): 2.56-2.61 (m, 8H), 2.81 (t, 4H, J=5.7 Hz), 3.72-3.76 (m, 8H), 4.15 (t, 4H, J=5.7 Hz), 5.77 (s, 1H), 6.49 (d, 2H, J=16.0 Hz), 6.92 (d, 4H, J=8.9 Hz), 7.50 (d, 4H, J=8.9 Hz), 7.61 (d, 2H, J=16.0 Hz), 16.00 (brs, 1H) MS (EI) m/z 534 (M+).
5-2

1H NMR (δ, CDCl3): 2.56-2.61 (m, 8H), 2.81 (t, 4H, J=5.7 Hz), 3.72-3.76 (m, 8H), 4.13 (t, 4H, J=5.7 Hz), 6.57 (s, 1H), 6.89 (d, 2H, J=16.4 Hz), 6.89 (d, 4H, J=8.8 Hz), 7.02 (d, 2H, J=16.4 Hz), 7.41 (d, 4H, J=8.8 Hz) MS (EI) m/z 530 (M+).
6-1

1H NMR (δ, CDCl3): 2.16 (s, 3H), 2.56-2.60 (m, 4H), 2.81 (t, 2H, J=5.7 Hz), 3.72-3.76 (m, 4H), 4.14 (t, 2H, J=5.7 Hz), 5.62 (s, 1H), 6.34 (d, 1H, J=15.7 Hz), 6.89-6.93 (m, 2H), 7.45-7.49 (m, 2H), 7.55 (d, 1H, J=15.8 Hz) MS (ESI) m/z 318(M+H)+.
6-2

1H NMR (δ, CDCl3): 2.57-2.61 (m, 4H), 2.82 (t, 2H, J=5.7 Hz), 2.99 (s, 3H), 3,10 (s, 3H), 3.72-3.77 (m, 4H), 4.15 (t, 2H, J=5.7 Hz), 4.74 (s, 2H), 5.79 (s, 1H), 6.50 (d, 2H, J=15.7 Hz), 6.90-6.99 (m, 4H), 7.48-7.54 (m, 4H), 7.61 (d, 1H, J=15.8 Hz), 7.62 (d, 1H, J=15.8 Hz) MS (ESI) m/z 508 (M+2H)+.
6-3

1H NMR (δ, DMSO-d6): 2.45-2.52 (m, 4H), 2.69 (t, 2H, J=5.8 Hz), 2.85 (s, 3H), 3.00 (s, 3H), 3.56-3.60 (m, 4H), 4.10 (t, 2H, J=5.7 Hz), 4.83 (s, 2H), 6.67 (s, 1H), 6.87-7.17 (m, 8H), 7.43-7.52 (m, 4H), 12.89 (s, 1H)
Table 6 (Compounds 7-2 and 8-2 are compounds of the invention; Compounds 7-1 and 8-1 are Reference Examples)
7-1

1H NMR (δ, CDCl3): 2.56-2.62 (m, 4H), 2.82 (t, 2H, J=5.7 Hz), 3.72-3.77 (m, 4H), 3.927 (s, 3H), 3.940 (s, 3H), 4.15 (t, 2H, J=5.6 Hz), 5.80 (s, 1H), 6.50 (d, 2H, J=15.8 Hz), 6.86-6.95 (m, 3H), 7.07-7.10 (m, 1H), 7.15 (dd, 1H, J=1.9, 8.3 Hz), 7.48-7.53 (m, 2H), 7.61 (d, 1H, J=15.8 Hz), 7.62 (d, 1H, J=15.9 Hz) MS (ESI) m/z 466 (M+H)+.
7-2

1H NMR (δ, CDCl3): 2.57-2.61 (m, 4H), 2.81 (t, 2H, J=5.8 Hz), 3.72-3.77 (m, 4H), 3.876 (s, 3H), 3.884 (s, 3H), 4.11 (t, 2H, J=5.7 Hz), 6.60 (s, 1H), 6.78-7.08 (m, 9H), 7.35-7.40 (m, 2H)
8-1

MS (ESI) m/z 446 (M+H)+.
8-2

1H NMR (δ, DMSO-d6): 2.45-2.53 (m, 4H), 2.69 (t, 2H, J=5.6 Hz), 3.56-3.61 (m, 4H), 4.08-4.13 (m, 2H), 6.72 (s, 1H), 6.90-7.35 (m, 6H), 7.42-7.85 (m, 5H), 8.19-8.26 (m, 1H), 12.49 (brs, 1H), 12.92 (brs, 1H)
Table 7 (Compounds 9-2 and 10-3 are compounds of the invention; Compounds 9-1, 10-1 and 10-2 are Reference Examples)
9-1

MS (EI) m/z 402 (M+).
9-2

1H NMR (δ, acetone-d6): 3.90 (s, 3H), 5.23 (s, 2H), 6.10-6.13 (m, 1H), 6.26-6.28 (m, 1H), 6.56 (s, 1H), 6.65 (dd, 1H, J=2.6 Hz, 8.4 Hz), 6.74 (d, 1H, J=2.6 Hz), 6.77 (d, 1H, J=16.8 Hz), 6.82-6.84 (m, 1H), 7.03 (d, 1H, J=16.8 Hz), 7.06 (d, 1H, J=16.8 Hz), 7.29-7.33 (m, 1H), 7.35 (d, 1H, J=16.8 Hz), 7.51 (d, 1H, J=8.4 Hz), 7.56 (br d, 1H, J=7.7), 7.82 (dt, 1H, J=1.8 Hz, 7.7 Hz), 8.57-8.60 (m, 1H), 10.33 (brs, 1H) MS (EI) m/z 398 (M+).
10-1

MS (EI) m/z 241 (M+).
10-2

MS (EI) m/z 506 (M+).
10-3

1H NMR (δ, CDCl3): 1.13 (t, 6H, J=7.2 Hz), 3.33 (q, 4H, J=7.2 Hz), 3.80 (s, 3H), 5.16 (s, 2H), 6.21 (d, 1H, J=2.0 Hz), 6.31 (dd, 1H, J=2.0 Hz, 8.7 Hz), 6.46 (d, 1H, J=3.1 Hz), 6.55 (s, 1H), 6.88 (d, 1H, J=16.4 Hz), 7.05 (d, 1H, J=3.1 Hz), 7.09 (d, 1H, J=16.4 Hz), 7.21-7.49 (m, 10H), 7.58 (1H, d, J=8.2 Hz) MS (EI) m/z 502 (M+).
Table 8 (Compounds 11-2 and 12-2 are compounds of the invention; Compounds 11-1 and 12-1 are Reference Examples)
11-1

MS (EI) m/z 529 (M+).
11-2

1H NMR (δ, CDCl3): 1.19 (t, 6H, J=7.2 Hz), 2.66-2.70 (m, 4H), 2.93 (t, 2H, J=5.9 Hz), 3.38 (q, 4H, J=7.2 Hz), 3.75-3.81 (m, 7H), 4.20 (t, 2H, J=5.9 Hz), 6.19 (d, 1H, J=2.5 Hz), 6.32 (dd, 1H, J=2.5 Hz, 8.7 Hz), 6.46 (d, 1H, J=3.1 Hz), 6.55 (s, 1H), 6.88 (d, 1H, J=16.4 Hz), 7.05 (d, 1H, J=3.1 Hz), 7.10 (d, 1H, J=16.4 Hz), 7.24 (d, 1H, J=16.4 Hz), 7.30 (d, 1H, J=16.4 Hz), 7.33 (dd, 1H, J=1.5 Hz, 8.2 Hz), 7.39 (d, 1H, J=8.7 Hz), 7.43 (brs, 1H), 7.58 (d, 1H, J=8.2 Hz) MS (EI) m/z 525 (M+).
12-1

MS (EI) m/z 452 (M+).
12-2

1H NMR (δ, acetone-d6): 3.87 (s, 3H), 5.17 (s, 2H), 6.41 (d, 1H, J=3.1 Hz), 6.57-6.61 (m, 2H), 6.69 (s, 1H), 7.09 (d, 1H, J=16.9 Hz), 7.15 (d, 1H, J=16.4 Hz), 7.23 (d, 1H, J=3.1 Hz), 7.29-7.33 (m, 2H), 7.34 (d, 1H, J=16.4 Hz), 7.41 (d, 1H, J=16.9 Hz), 7.48 (d, 1H, J=8.2 Hz), 7.52 (br d, 1H, J=7.7 Hz), 7.54 (d, 1H, J=8.7 Hz), 7.56 (brs, 1H), 7.81 (dt, 1H, J=2.1 Hz, 7.7 Hz), 8.56-8.59 (m, 1H), 8.13-9.43 (br, 1H), 11.20-12.53 (br, 1H) MS (EI) m/z 448 (M+).
Table 9 (Compound 13-3 is a compound of the invention; Compounds 13-1 and 13-2 are Reference Examples)
13-1

1H NMR (δ, CDCl3): 2.56-2.61 (m, 4H), 2.85 (t, 2H, J=5.6 Hz), 3.71-3.74 (m, 4H), 4.18 (t, 2H, J=5.6 Hz), 5.26 (s, 2H), 6.57-6.59 (m, 1H), 6.62-6.65 (m, 1H), 7.24-7.28 (m, 1H), 7.47-7.50 (m, 1H), 7.72-7.76 (m, 1H), 7.81 (d, 1H, J=8.7 Hz), 8.61-8.63 (m, 1H), 10.31 (s, 1H)
13-2

MS (EI) m/z 551 (M+)
13-3

1H NMR (δ, DMSO-d6): 2.49-2.56 (m, 4H), 2.77-2.82 (m, 2H), 3.59-3.63 (m, 4H), 4.17 (t, 2H, J=5.7 Hz), 5.22 (s, 2H), 6.40-6.44 (m, 1H), 6.61-6.69 (m, 2H), 6.75-6.79 (m, 1H), 6.98-7.10 (m, 2H), 7.20-7.31 (m, 3H), 7.34-7.40 (m, 2H), 7.46-7.57 (m, 4H), 7.84-7.88 (m, 1H), 8.58-8.61 (m, 1H), 11.18 (brs, 1H), 12.87 (brs, 1H) MS (EI) m/z 547 (M+).
Table 10 (Compounds 1-2 and 2-2 are compounds of the invention; Compounds 1-1 and 2-1 are Reference Examples)
Ex.St.
Dat.
14-1

1H NMR (δ, CDCl3): 2. 56-2. 60 (m, 4H), 2.82 (t, 2H, J=5. 8 Hz), 3. 72-3. 76 (m, 4H), 3.83 (s, 3H), 3.88 (s, 3H), 4.15 (t, 2H, J=5. 8 Hz), 5.83 (s, 1H), 6.48-6.53 (m, 3H), 6.64 (d, 1H, J=16. 0 Hz), 6. 66 (d, 1H, J=16. 0 Hz), 7.12 (d, 1H, J=3.2 Hz), 7.38 (dd, 1H, J=1.3, 8.3 Hz), 7.47-7.49 (m, 2H), 7.60 (d, 1H, J=8.3 Hz), 7.81 (d, 1H, J=16.0 Hz), 7.90 (d, 1H, J=16.0 Hz) MS (EI) m/z 488 (M+).
14-2

1H NMR (δ, CDCl3): 2. 59-2. 63 (m, 4H), 2.83 (t, 2H, J=5. 7 Hz), 3. 75-3. 78 (m, 4H), 3.79 (s, 3H), 3.86 (s, 3H), 4.14 (t, 2H, J=5. 7 Hz), 6. 45-6. 50 (m, 3H), 6.64 (s, 1H), 6.99 (d, 1H, J=16. 6 Hz), 7.06 (d, 1H, J=3.1 Hz), 7.09 (d, 1H, J=16.5 Hz), 7.24 (d, 1H, J=16.6 Hz), 7.30-7.35 (m, 2H), 7.40-7.42 (m, 1H), 7.45 (d, 1H, J=9.2 Hz), 7.58 (d, 1H, J=8.2 Hz) , MS (EI) m/z 484 (M+).
15-1

MS (EI) m/z 458 (M+).
15-2

1H NMR (δ, CDCl3) : 2.57-2.61 (m, 4H), 2.82 (t, 2H, J=5.7 Hz), 3. 73-3. 76 (m, 4H), 3.80 (s, 3H), 4.14 (t, 2H, J=5.7 Hz), 6.46-6.47 (m, 1H), 6.61 (s, 1H), 6.88-6.97 (m, 3H), 7.02-7.08 (m, 3H), 7.23 (d, 1H, J=16.5 Hz), 7.31 (dd, 1H, J=1.4, 8.3 Hz), 7.40-7.44 (m, 2H), 7.59 (d, 1H, J=8.3 Hz), 7.77 (d, 1H, J=8.7 Hz) MS (EI) m/z 454 (M+).
Table 11 (Compounds 16-2 and 17-2 are compounds of the invention; Compounds 16-1 and 17-1 are Reference Examples)
16-1

MS (EI) m/z 454 (M+).
16-2

1H NMR (δ, CDCl3): 3.80 (s, 3H), 5.21 (s, 2H), 6.47 (dd, 1H, J=0.7Hz, 3.2 Hz), 6.65 (s, 1H), 6.73 (dd, 1H, J=2.5 Hz, 12.3 Hz), 6.79 (dd, 1H, J=2.5 Hz, 8.7 Hz), 7.02 (d, 1H, J=16. 7 Hz), 7.05 (d, 1H, J=16.9 Hz), 7.06 (d, 1H, J=3.0 Hz), 7.16 (d, 1H, J=16. 7 Hz), 7.21-7.26 (m, 2H), 7.32 (dd, 1H, J=1.35 Hz, 8.3Hz), 7.40 (s, 1H), 7.45-7.51 (m, 2H), 7.59 (d, 1H, J=8. 3 Hz), 7.70-7.75 (m, 1H), 8.62 (d, 1H, J=4.3 Hz) MS (EI) m/z 450 (M+).
17-1

1H NMR (δ, CDCl3): 3.84 (s, 3H), 5.25 (s, 2H), 5.82 (s, 1H), 6.49-6.53 (m, 2H), 6.67 (d, 1H, J=15.7 Hz), 7.00-7.02 (m, 2H), 7.13 (d, 1H, J=3.0 Hz), 7.23-7.27 (m, 1H), 7.39 (dd, 1H, J=1.2, 8.4 Hz), 7.48-7.53 (m, 4H), 7.60-7.64 (m, 2H), 7.71-7.75 (m, 1H), 7.83 (d, 1H, J=15. 7 Hz), 8.60-8.63 (m, 1H), 16.11 (brs, 1H), MS (EI) m/z 436 (M+).
17-2

1H NMR (δ, CDCl3): 3.77 (s, 3H), 5.22 (s, 2H), 6.46 (d, 1H, J=3.0 Hz), 6.62 (s, 1H), 6.91-6.97 (m, 3H), 7.04-7.10 (m, 3H), 7.22-7.27 (m, 2H), 7.31 (dd, 1H, J=1.0, 8.3 Hz), 7.40-7.43 (m, 3H), 7.51-7.53 (m, 1H), 7.58 (d, 1H, J=8.3 Hz), 7.70-7.74 (m, 1H), 8.60-8.62 (m, 1H), MS (EI) m/z 432 (M+).
Table 12 (Compound 18-2 is a compound of the invention; Compound 18-1 is Reference Example)
18-1

1H NMR (δ, CDCl3) 2.35 (s, 3H), 2.56 (t, 4H, J=4.9 Hz), 3.32 (t, 4H, J=4.9 Hz), 3.83 (s, 3H), 5.81 (s, 1H), 6.44-6.51 (m, 2H), 6.65 (d, 1H, J=15. 7 Hz), 6.89 (d, 2H, J=8.6 Hz), 7.12 (d, 1H, J=2.9 Hz), 7.38 (d, 1H, J=8.3 Hz), 7.44-7.50 (m, 3H), 7.58-7.64 (m, 2H), 7.81 (d, 1H, J=15. 7 Hz), 16. 15 (brs, 1H) MS (EI) m/z 427 (M+).
18-2

1H NMR (δ, CDCl3): 2.38 (s, 3H), 2.57-2.64 (m, 4H), 3.24-3.31 (m, 4H), 3.80 (s, 3H), 6.45-6.48 (m, 1H), 6.60-6.62 (m, 1H), 6.87-6.92 (m, 3H), 6.99-7.10 (m, 3H), 7.20-7.27 (m, 1H), 7.32 (d, 1H, J=8.5 Hz), 7.39-7.43 (m, 3H), 7.57-7.61 (m, 1H) MS (EI) m/z 423 (M+).
Table 13 (Compound 19-4 is a compound of the invention; Compounds 19-1 to 19-3 are Reference Examples)
19-1

1H NMR (δ, DMSO-d6) : 5.25 (s. 2H), 6.46 (d, 1H, J=16. 1 Hz), 6.92-6.96 (m, 1H), 7.02-7.07 (m, 1H), 7.35-7.39 (m, 1H), 7.52-7.54 (m, 1H), 7.59 (d, 1H, J=16. 1 Hz), 7.76-7.80 (m, 1H), 7.83-7.88 (m, 1H), 8.58-8.60 (m, 1H), 12.41 (brs, 1H) MS (EI) m/z 273 (M+).
19-2

1H NMR (δ, CDCl3) : 2. 16 (s, 3H), 5. 23 (s, 2H), 5. 63-5. 64 (m, 1H), 6. 44-6. 48 (m, 1H), 6.72-6.76 (m, 1H), 6.79-6.82 (m, 1H), 7.25-7.29 (m, 1H), 7. 42-7. 47 (m, 1H), 7.48-7.50 (m, 1H), 7.63 (d, 1H, J=16. 1 Hz), 7.72-7.77 (m, 1H), 8.60-8.63 (m, 1H) MS (EI) m/z 313 (M+).
19-3

1H NMR (δ, CDCl3): 3.74 (s, 3H), 5.23 (s, 2H), 5.72 (s, 1H), 6.19-6.22 (m, 1H), 6.37 (d, 1H, J=15. 4 Hz), 6.59 (d, 1H, J=16. 1 Hz), 6. 71-6. 77 (m, 2H), 6.77-6.79 (m, 1H), 6.81 (dd, 1H, J=2.5Hz, 8.8 Hz), 7.24-7.28 (m, 1H), 7.45-7.51 (m, 2H), 7.61 (d, 1H, J=15.4Hz), 7.67 (d, 1H, J=16.1 Hz), 7.72-7.76 (m, 1H), 8.61-8.63 (m, 1H), 16.10 (brs, 1H) MS (EI) m/z 404 (M+).
19-4

1H NMR (δ, CDCl3) : 3.70 (s, 3H), 5.21 (s, 2H), 6.14-6.16 (m, 1H), 6.50 (dd, 1H, J=1.4Hz, 3.8 Hz), 6.58 (s, 1H), 6.65 (t, 1H, J=2.1 Hz), 6.71-6.81 (m, 3H), 6.94-7.02 (m, 2H), 7.14 (d, 1H, J=16.7 Hz), 7.24-7.27 (m, 1H), 7.47 (t, 1H, J=8.7 Hz), 7.50 (d, 1H, J=8.0 Hz), 7.71-7.75 (m, 1H), 8.62 (d, 1H, J=4.9 Hz) MS (EI) m/z 400 (M+).
Table 14 (Compound 20-3 is a compound of the invention; Compounds 20-1 and 20-2 are Reference Examples)
20-1

1H NMR (δ, CDCl3) : 2.15 (s, 3H), 5.24 (s, 2H), 5.61 (s, 1H), 6.34 (d, 1H, J=16. 0 Hz), 6.99 (d, 2H, J=8.7 Hz), 7.23 (dd, 1H, J=5.0Hz, 7.6 Hz), 7.47 (d, 2H, J=8.7 Hz), 7.48-7.51 (m, 1H), 7.55 (d, 1H, J=16.0 Hz), 7.69-7.73 (m, 1H), 8.60-8.62 (m, 1H), 15.43 (brs, 1H)
20-2

1H NMR (δ, CDCl3) : 3.74 (s, 3H), 5.25 (s, 2H), 5.71 (s, 1H), 6.19-6.21 (m, 1H), 6.37 (d, 1H, J=15.4Hz), 6.48 (d, 1H, J=15.8Hz), 6.71-6.73 (m, 1H), 6.77-6.78 (m, 1H), 7.00 (d, 2H, J=8.8 Hz), 7.23-7.27 (m, 1H), 7.48-7.52 (m, 1H), 7.50 (d, 2H, J=8.8 Hz), 7.59 (d, 1H, J=15. 8 Hz), 7.60 (d, 1H, J=15.4 Hz), 7.71-7.75 (m. 1H), 8.61 (d, 1H, J=4.2 Hz) MS (EI) m/z 386 (M+).
20-3

1H NMR (δ, CDCl3) 3.69 (s, 3H), 5.23 (s, 2H), 6.15 (t, 1H, J=3.2 Hz), 6.49 (dd, 1H, J=1.5Hz, 3. 7 Hz), 6.54 (s, 1H), 6.64-6.66 (m, 1H), 6.76 (d, 1H, J=16. 2 Hz), 6.90 (d, 1H, J=16. 5 Hz), 6.96 (d, 1H, J=16.2 Hz), 6.98 (d, 2H, J=8.8 Hz), 7.02 (d, 1H, J=16.5 Hz), 7.22-7.26 (m, 1H), 7.42 (d, 2H, J=8. 8 Hz), 7.52 (d, 1H, J=7.9 Hz), 7.70-7.74 (m, 1H), 8.60-8.62 (m, 1H) MS (EI) m/z 382 (M+).
Table 15 (Compounds 21-2 and 22-2 are compounds of the invention; Compounds 21-1 and 22-1 are Reference Examples)
21-1

MS (EI) m/z 438 (M+).
21-2

1H NMR (δ, CDCl3) : 2.57-2.60 (m, 4H), 2.81 (t, 2H, J=5.7 Hz), 3.69 (s, 3H), 3. 73-3. 76 (m, 4H), 3.86 (s, 3H), 4.13 (t, 2H, J=5.7 Hz), 6. 13-6. 15 (m, 1H), 6.47-6.51 (m, 3H), 6.54 (s, 1H), 6.63-6.64 (m, 1H), 6.77 (d, 1H, J=16. 0 Hz), 6.95 (d, 1H, J=16. 5 Hz), 6.96 (d, 1H, J=16. 0 Hz), 7.29 (d, 1H, J=16.5 Hz), 7.43 (d, 1H, J=8.3 Hz) MS (EI) m/z 434 (M+).
22-1

MS (EI) m/z 416 (M+).
22-2

1H NMR (δ, CDCl3): 3.68 (s, 3H), 3.84 (s, 3H), 5.23 (s, 2H), 6.13-6.15 (m, 1H), 6.47 (dd, 1H, J=1.4, 3.4 Hz), 6.54 (s. 1H), 6.55-6.59 (m, 2H), 6.62-6.63 (m, 1H), 6.77 (d, 1H, J=16.0 Hz), 6.95 (d, 1H, J=16.5 Hz), 6.96 (d, 1H, J=16.0 Hz), 7.22-7.25 (m, 1H), 7.29 (d, 1H, J=16. 5 Hz), 7.42 (d, 1H, J=8.7 Hz), 7.51-7.53 (m, 1H), 7.70-7.74 (m, 1H), 8.60-8.62 (m, 1H) MS (EI) m/z 412 (M+).
Table 16 (Compounds 23-2 and 24-2 are compounds of the invention; Compounds 23-1 and 24-1 are Reference Examples)
23-1

1H NMR (δ, CDCl3): 2.28 (s, 3H), 3.59 (s, 3H), 3.86 (s, 3H), 5.25 (s, 2H), 5.69 (s, 1H), 5.99 (d, 1H, J=3.9 Hz), 6.32 (d, 1H, J=15.5 Hz), 6.56-6.61 (m, 3H), 6.66 (d, 1H, J=3.9 Hz), 7.24-7.28 (m, 1H), 7.46 (d, 1H, J=9.3Hz), 7.50-7.53 (m, 1H), 7.60 (d, 1H, J=15. 5 Hz), 7.71-7.76 (m, 1H), 7.85 (d, 1H, J=16.2 Hz), 8.60-8.63 (m, 1H) MS (EI) m/z 430 (M+).
23-2

1H NMR (δ, CDCl3): 2.25 (s, 3H), 3.56 (s, 3H), 3.85 (s, 3H), 5.23 (s, 2H), 5.90-5.95 (m, 1H), 6.40-6.45 (m, 1H), 6.52-6.61 (m, 3H), 6.71 (d, 1H, J=16.5Hz), 6.97 (d, 1H, J=17.0Hz), 7.03 (d, 1H, J=16.5Hz), 7.21-7.35 (m, 2H), 7.42 (d, 1H, J=8.5 Hz), 7.50-7.54 (m, 1H), 7.69-7.76 (m, 1H), 8.68-8.64 (m, 1H) MS (EI) m/z 426 (M+).
24-1

1H NMR (δ, CDCl3) : 2.24 (s, 3H), 2.27 (s, 3H), 3.58 (s, 3H), 3.86 (s. 3H), 5.24 (s, 2H), 5.70 (s, 1H), 5.86 (s, 1H), 6.18 (d, 1H, J=15. 5 Hz) , 6.56-6.60 (m, 3H), 7.23-7.27 (m, 1H), 7.46 (d, 1H, J=9.2 Hz), 7.49-7.53 (m, 1H), 7.69-7.75 (m, 2H), 7.84 (d, 1H, J=16. 1 Hz), 8.60-8.63 (m, 1H) MS (EI) m/z 444 (M+).
24-2

1H NMR (δ, CDCl3) : 2.22 (s, 3H), 2.23 (s, 3H), 3.54 (s, 3H), 3.86 (s, 3H), 5.24 (s, 2H), 5.80 (s, 1H), 6.52-6.60 (m, 4H), 6.97 (d, 1H, J=16.7 Hz), 7.01 (d, 1H, J=16.7 Hz), 7.23-7.27 (m, 1H), 7.30 (d, 1H, J=16.7 Hz), 7.44 (d, 1H, J=8.3 Hz), 7.51-7.54 (m, 1H), 7.71-7.75 (m, 1H), 8.61-8.63 (m, 1H) MS (EI) m/z 440 (M+).
Table 17 (Compounds 25-2 and 26-2 are compounds of the invention; Compounds 25-1 and 26-1 are Reference Examples)
25-1

1H NMR (δ, CDCl3) : 2.60 (s, 3H), 3.88 (s, 3H), 5.25 (s, 2H), 5.92 (s, 1H), 6.58-6.61 (m, 2H), 6.66 (d, 1H, J=16.0 Hz), 7.09-7.12 (m, 1H), 7.17 (d, 1H, J=15.5 Hz), 7.20-7.23 (m, 1H), 7.24-7.27 (m, 1H), 7.48-7.53 (m, 2H), 7.56-7.61 (m, 2H), 7.71-7.76 (m, 1H), 7.93 (d, 1H, J=16.0 Hz), 8.61-8.63 (m, 1H), 15.85 (brs, 1H) MS (EI) m/z 428 (M+).
25-2

1H NMR (δ, CDCl3) : 2.59 (s, 3H), 3.86 (s, 3H), 5.24 (s, 2H), 6. 56-6. 60 (m, 2H), 6.68 (s, 1H), 6.97 (d, 1H, J=16. 6 Hz), 7.02-7.04 (m, 1H), 7.16 (d, 1H, J=16. 2 Hz), 7.23-7.27 (m, 2H), 7.30 (d, 1H, J=16. 6 Hz), 7.44 (d, 1H, J=8.3 Hz), 7.51-7.58 (m, 3H), 7.71-7.75 (m, 1H), 8.61-8.63 (m, 1H) MS (EI) m/z 424 (M+).
26-1

MS (EI) m/z 428 (M+).
26-2

1H NMR (δ, CDCl3) : 2.67 (s, 3H), 3.86 (s, 3H), 5.24 (s, 2H), 6.56-6.61 (m, 2H), 6.65 (s, 1H), 6.95 (d, 1H, J=16. 4 Hz), 7.00 (d, 1H, J=16. 4 Hz), 7.15 (dd, 1H, J=4.7, 7.9 Hz), 7.23-7.28 (m, 2H), 7.31 (d, 1H, J=16. 4 Hz), 7.43 (d, 1H, J=8.6 Hz), 7.53 (d, 1H, J=7.9 Hz), 7.74 (dt, 1H, J=1.9, 7.6 Hz), 7.83 (dd, 1H, J=1.6, 7.9 Hz), 8.40 (dd, 1H, J=1.6, 4.7 Hz), 8.61-8.63 (m, 1H) MS (EI) m/z 424 (M+).
Table 18 (Compound 27-3 is a compound of the invention; Compounds 27-1 and 27-2 are Reference Examples)
27-1

1H NMR (δ, CDCl3) : 2.12 (s, 3H), 3.71 (s, 3H), 5.55 (s, 1H), 6.17-6.19 (m, 1H), 6.20 (d, 1H, J=15.3 Hz), 6.65-6.66 (m, 1H), 6.74-6.75 (m, 1H), 7.52 (d, 1H, J=15.3 Hz), 15.63 (brs, 1H)
27-2

1H NMR (δ, CDCl3) : 2.85 (s, 3H), 3.06 (t, 4H, J=5.0 Hz), 3.82 (t, 4H, J=5.0 Hz), 4.22 (s, 3H), 6.19 (s, 1H), 6.68-6.70 (m, 1H), 6.85 (d, 1H, J=15. 4 Hz), 6.94 (d, 1H, J=16. 0 Hz), 7.19 (dd, 1H, J=1.3 Hz, 3.9 Hz), 7.24-7.26 (m, 1H), 7.38 (d, 2H, J=8.9 Hz), 7.95 (d, 2H, J=8.9 Hz), 8.07 (d, 1H, J=16.0 Hz), 8.08 (d, 1H, J=15.4 Hz), 16.68 (brs, 1H) MS (EI) m/z 377 (M+).
27-3

1H NMR (δ, CDCl3) : 2.38 (s, 3H), 2.58-2.64 (m, 4H), 3.28 (t, 4H, J=4.8 Hz), 3.69 (s, 3H), 6.15 (dd, 1H, J=2.8 Hz, 3.6 Hz), 6.48 (dd, 1H, J=1.5 Hz, 3.6 Hz), 6.52 (s, 1H), 6.63-6.65 (m, 1H), 6.76 (d, 1H, J=16.2 Hz), 6.86 (d, 1H, J=16.4 Hz), 6.90 (d, 2H, J=8.8 Hz), 6.95 (d, 1H, J=16.2 Hz), 6.99 (d, 1H, J=16.4 Hz), 7.39 (d, 2H, J=8.8 Hz) MS (EI) m/z 373 (M+).
Table 19 (Compounds 28-2 and 29-2 are compounds of the invention; Compounds 28-1 and 29-1 are Reference Examples)
28-1

1H NMR (δ, CDCl3) : 2.02 (quint, 2H, J=5.9 Hz), 2.38 (s, 3H), 2.54-2.58 (m, 2H), 2.69-2.73 (m, 2H), 3.52-3. 56 (m, 2H), 3. 60-3. 64 (m, 2H), 3.73 (s, 3H), 5.68 (s, 1H), 6.18-6.21 (m, 1H), 6.36 (d, 1H, J=15.5 Hz), 6.40 (d, 1H, J=15.7 Hz), 6.66-6.70 (m, 3H), 6.75-6.77 (m, 1H), 7.42-7.45 (m, 2H), 7.57 (d, 1H, J=15.5 Hz), 7.59 (d, 1H, J=15.6 Hz) MS (EI) m/z 391 (M+).
28-2

1H NMR (δ, CDCl3): 2.00-2.06 (m, 2H), 2.39 (s, 3H), 2.55-2.60 (m, 2H), 2.70-2.74 (m, 2H), 3.50-3.54 (m, 2H), 3.59-3.62 (m, 2H), 3.69 (s, 3H), 6. 14-6. 16 (m, 1H), 6.47-6.53 (m, 2H), 6.63-6.69 (m, 3H), 6.78 (d, 1H, J=16. 1 Hz), 6.79 (d, 1H, J=16. 4 Hz), 6.96 (d, 1H, J=16. 1 Hz), 6.98 (d, 1H, J=16.4 Hz), 7.34-7.38 (m, 2H) MS (EI) m/z 387 (M+).
29-1

1H NMR (δ, CDCl3): 1.36 (d, 6H, J=5.8 Hz), 3.73 (s, 3H), 3.86 (s, 3H), 4. 57-4. 63 (m, 1H), 5.71 (s. 1H), 6.18-6.20 (m, 1H), 6.36 (d, 1H, J=15.4 Hz), 6.44 (d, 1H, J=2.4 Hz), 6.49 (dd, 1H, J=2.4 Hz, 8.5 Hz), 6.59 (d, 1H, J=15.9 Hz), 6.68-6.70 (m, 1H), 6.74-6.76 (m, 1H), 7.45 (d, 1H, J=8.5 Hz), 7.58 (d, 1H, J=15.4 Hz), 7.87 (d, 1H, J=15.9Hz) MS (EI) m/z 367 (M+).
29-2

1H NMR (δ, CDCl3) : 1.35 (d, 6H, J=6.1 Hz), 3.69 (s, 3H), 3.85 (s, 3H), 4.53-4.61 (m, 1H), 6.13-6.15 (m, 1H), 6.45 (d, 1H, J=2.3 Hz), 6.46-6.49 (m, 2H), 6.54 (s, 1H), 6.62-6.64 (m, 1H), 6.77 (d, 1H, J=16.2 Hz), 6.95 (d, 1H, J=16. 7 Hz), 7.01 (d, 1H, J=16.2 Hz), 7.30 (d, 1H, J=16. 7 Hz), 7.41 (d, 1H, J=8.5 Hz) MS (EI) m/z 363 (M+).
Table 20 (Compound 30-3 is a compound of the invention; Compounds 30-1 and 30-2 are Reference Examples)
30-1

1H NMR (δ, CDCl3) : 2.30 (s, 3H), 2.40-2.66 (m, 10H), 2.86-2.90 (m, 2H), 7.37 (d, 2H, J=8.0 Hz), 7.80 (d, 2H, J=8.0Hz), 9.98 (s, 1H)
30-2

MS (EI) m/z 405 (M+).
30-3

1H NMR (δ, CDCl3) : 2.30 (s, 3H), 2.43-2.66 (m, 10H), 2. 79-2. 83 (m, 2H), 3.70 (s, 3H), 6.14-6.16 (m, 1H), 6.49 (dd, 1H, J=1.4 Hz, 3.7 Hz), 6.55 (s, 1H), 6.64-6.65 (m, 1H), 6.75 (d, 1H, J=16. 3 Hz), 6.95 (d, 1H, J=16. 5 Hz), 6.98 (d, 1H, J=16. 3 Hz), 7.05 (d, 1H, J=16. 5 Hz), 7.20 (d, 2H, J=8. 1 Hz), 7.40 (d, 2H, J=8.1 Hz) MS (EI) m/z 401 (M+).
Table 21 (Compound 31-3 is a compound of the invention; Compounds 31-1 and 31-2 are Reference Examples)
31-1

MS (EI) m/z 257 (M+).
31-2

1H NMR (δ, CDCl3): 3.28 (t, 2H, J=6.6Hz), 3.73 (s, 3H), 3.86 (s, 3H), 4.42 (t, 2H, J=6. 6 Hz), 5.71 (s, 1H), 6.17-6.21 (m, 1H), 6.36 (d, 1H, J=15.4 Hz), 6.45 (d, 1H, J=2.0 Hz), 6.53 (dd, 1H, J=2.0Hz, 8.6 Hz), 6.59 (d, 1H, J=16.0 Hz), 6.90 (d, 1H, J=3.68 Hz), 6.75 (s, 1H), 7.13-7.18 (m, 1H), 7.24-7.28 (m, 1H), 7.45 (d, 1H, J=8.6 Hz), 7.58 (d, 1H, J=15.4 Hz), 7.60-7.66 (m, 1H), 7.87 (d, 1H, J=16. 0 Hz), 8.57 (d, 1H, J=4.7 Hz), 16.18 (brs, 1H) MS (EI) m/z 430 (M+).
31-3

1H NMR (δ, CDCl3): 3.28 (t, 2H, J=6.7Hz), 3.70 (s, 3H), 3.84 (s, 3H), 4.40 (t, 2H, J=6.7 Hz), 6.15 (t, 1H, J=3.3 Hz), 6.45 (d, 1H, J=2.2 Hz), 6.48-6.52 (m, 2H), 6.55 (s, 1H), 6.63-6.65 (m, 1H), 6.77 (d, 1H, J=16.5 Hz), 6.94 (d, 1H, J=16.9 Hz), 6.99 (d, 1H, J=16.5 Hz), 7.17 (dd, 1H, J=5.1Hz, 7.45 Hz), 7.25-7.32 (m, 2H), 7.41 (d, 1H, J=8.6 Hz), 7.62-7.66 (m, 1H), 8. 56-8. 58 (m, 1H) MS (EI) m/z 426 (M+).
Table 22 (Compounds 32-1 to 32-4 are Reference Examples)
32-1

1H NMR (δ, CDC l3) : 2. 59 (s, 3H), 3.85 (s, 3H), 5.24 (s, 2H), 6.82 (dd, 1H, J=2. 5Hz, 8.8 Hz), 6.85 (d, 1H, J=2.5 Hz), 7.22-7.26 (m, 1H), 7.48-7.51 (m, 1H), 7.70-7.74 (m, 1H), 7.93 (d, 1H, J=8.7 Hz), 8.60-8.62 (m, 1H) MS (EI) m/z 257 (M+).
32-2

1H NMR (δ, CDCl3,): 2.36 (s, 3H), 4.64 (s, 2H), 5.19 (s, 2H), 6.78 (dd, 1H, J=2.7Hz, 8.4Hz), 6.85 (d, 1H, J=2.6Hz), 7.21-7.25 (m, 2H), 7.50-7.53 (m, 1H), 7.69-7.73 (m, 1H), 8.58-8.61 (m, 1H) MS (EI) m/z 229 (M+).
32-3

1H NMR (δ, CDCl3,): 2.65 (s, 3H), 5.27 (s, 2H), 6.86 (d, 1H, J=2.4 Hz), 6.93 (dd, 1H, J=2.6Hz, 8.5 Hz), 7.24-7.27 (m, 1H), 7.48-7.50 (m, 1H), 7.71-7.77 (m, 2H), 8.61-8.63 (m, 1H), 10.12 (s, 1H) MS (EI) m/z 227 (M+).
32-4

1H NMR (δ, CDCl3): 2.44 (s, 3H), 3.74 (s, 3H), 5.23 (s, 2H), 5.70 (s, 1H), 6.19-6.21 (m, 1H), 6.37 (d, 1H, J=15.5Hz), 6.43 (d, 1H, J=15.5Hz), 6.71-6.73 (m, 1H). 6.77-6.78 (m, 1H), 6.83-6.86 (m, 2H), 7.22-7.27 (m, 1H), 7.49-7.52 (m, 1H), 7.55-7.58 (m, 1H), 7.60 (d, 1H, J=15.5 Hz), 7.70-7.74 (m, 1H), 7.86 (d, 1H, J=15.6 Hz), 8.60-8.62 (m, 1H) MS (EI) m/z 400 (M+).
Table 23 (Compound 32-5 is a compound of the invention)
32-5

1H NMR (δ, CDCl3): 2.40 (s, 3H), 3.70 (s, 3H), 5.22 (s, 2H), 6.15-6.16 (m, 1H), 6.49 (dd, 1H, J=1.6Hz, 3.8 Hz), 6.55 (s, 1H), 6. 64-6. 66 (m, 1H), 6.77 (d, 1H, J=16. 3 Hz), 6.80-6.85 (m, 3H), 6.96 (d, 1H, J=16. 3 Hz), 7.21-7.25 (m, 2H), 7.49-7.53 (m, 2H), 7.70-7.74 (m, 1H), 8.60-8.62 (m, 1H) MS (EI) m/z 396 (M+).
Table 24 (Compound 33-2 is a compound of the invention; Compound 33-1 is a Reference Example)
33-1

1H NMR (δ, CDCl3): 3.30 (t, 2H, J=6.6 Hz), 3.74 (s, 3H), 4.42 (t, 2H, J=6.6 Hz), 5.73 (s, 1H), 6.20-6.22 (m, 1H), 6.38 (d, 1H, J=15. 3 Hz), 6.56 (d, 1H, J=15. 9 Hz), 6. 72-6. 74 (m, 1H), 6. 78-6. 79 (m, 1H), 6. 90-6. 93 (m, 1H), 7.06-7.08 (m, 1H), 7.11-7.13 (m, 1H), 7.16-7.20 (m, 1H), 7.25-7.31 (m, 2H), 7.56 (d, 1H, J=15.8 Hz), 7.62 (d, 1H, J=15.6 Hz), 7.63-7.67 (m, 1H), 8.56-8.59 (m, 1H), 16.07 (brs, 1H), MS (EI) m/z 400 (M+).
33-2

1H NMR (δ, CDCl3) : 3.31 (t, 2H, J=6.6Hz), 3.70 (s, 3H), 4.40 (t, 2H, J=6.6 Hz), 6.14-6.17 (m, 1H), 6.49-6.52 (m, 1H), 6.57 (s, 1H), 6.64-6.67 (m, 1H), 6.73-6.78 (m, 1H), 6.81-6.85 (m, 1H), 6.98 (d, 1H, J=16.2 Hz), 7.01-7.08 (m, 4H), 7.17-7.21 (m, 1H), 7.22-7.28 (m, 1H), 7.30-7.34 (m, 1H), 7.64-7.69 (m, 1H), 8.56-8.59 (m, 1H) MS (EI) m/z 396 (M+).
Table 25 (Compound 34-3 is a compound of the invention; Compounds 34-1 and 34-2 are Reference Examples)
34-1

1H NMR (δ, CDCl3) : 3.85 (s, 6H), 50028 (s, 2H), 6.20 (s, 2H), 7.25-7.28 (m, 1H), 7.51 (d, 1H, J=7.8 Hz), 7.73-7.77 (m, 1H), 8.60-8.62 (m, 1H), 10.35 (s. 1H) MS (EI) m/z 273 (M+).
34-2

1H NMR (δ, CDCl3) : 3.72 (s, 3H), 3.85 (s, 6H), 5.25 (s, 2H), 5.71 (s, 1H), 6. 17-6. 19 (m, 1H), 6.23 (s, 2H), 6.36 (d, 1H, J=15. 4 Hz), 6. 66-6. 68 (m, 1H), 6.73-6.75 (m, 1H), 6.97 (d, 1H, J=16.0 Hz), 7.23-7.26 (m, 1H), 7.51 (d, 1H, J=7.7Hz), 7.56 (d, 1H, J=15.4Hz), 7.71-7.75 (m, 1H), 8.03 (d, 1H, J=16.0 Hz), 8.61 (d, 1H, J=4.5 Hz), 16.26 (brs, 1H) MS (EI) m/z 446 (M+).
34-3

1H NMR (δ, CDCl3) : 3.69 (s, 3H), 3.84 (s, 6H), 5.24 (s, 2H), 6. 13-6. 15 (m, 1H), 6.26 (s, 2H), 6.47 (dd, 1H, J=1.6Hz, 3.7 Hz), 6.54 (s, 1H), 6.61-6.63 (m, 1H), 6.80 (d, 1H, J=16.2 Hz), 6.97 (d, 1H, J=16.2 Hz), 7.22-7.26 (m, 1H), 7.34 (s, 2H), 7.53 (d, 1H, J=7.9 Hz), 7.71-7.75 (m, 1H), 8.61 (d, 1H, J=5.0 Hz) MS (EI) m/z 442 (M+).
Table 26 (Compounds 35-2 and 36-2 are compounds of the invention; Compounds 35-1 and 36-1 are Reference Examples)
35-1

1H NMR (δ, CDCl3): 3.76 (s, 3H), 3.87 (s, 3H), 5.25 (s, 2H). 5.81 (s, 1H), 6.58-6.61 (m, 2H), 6.64 (d, 1H, J=16. 0 Hz), 6.95-6.96 (m, 1H), 7.04 (d, 1H, J=15. 1 Hz), 7.16-7.17 (m, 1H), 7.22-7.26 (m, 1H), 7.44 (d, 1H, J=15.1 Hz), 7. 47-7. 52 (m, 2H), 7. 70-7. 74 (m, 1H), 7.93 (d, 1H, J=16. 0 Hz), 8.60-8.62 (m, 1H), 15.93 (brs, 1H) MS (EI) m/z 417 (M+).
35-2

1H NMR (δ, CDCl3) : 3.71 (s, 3H), 3.85 (s, 3H), 5.23 (s, 2H), 6.56-6.60 (m, 3H), 6.86-6.88 (m, 1H), 6.96 (d, 1H, J=16. 8 Hz), 6.97 (d, 1H, J=16. 1 Hz), 7.09-7.11 (m, 1H), 7.22-7.25 (m, 1H), 7.29 (d, 1H, J=16.8 Hz), 7.42 (d, 1H, J=8.3Hz), 7.46 (d, 1H, J=16. 1 Hz), 7.51-7.53 (m, 1H), 7.70-7.74 (m, 1H), 8. 59-8. 62 (m, 1H) MS (EI) m/z 413 (M+).
36-1

MS (EI) m/z 417 (M+).
36-2

1H NMR (δ, CDCl3) :3.70 (s, 3H), 3.86 (s, 3H), 5.24 (s, 2H), 6.56-6.60 (m, 3H), 6.89-6.90 (m, 2H), 6.93 (d, 1H, J=16.5 Hz), 7.23-7.31 (m, 3H), 7.42 (d, 1H, J=8.2 Hz), 7.43-7.44 (m, 1H), 7.51-7.54 (m, 1H), 7.71-7.75 (m, 1H), 8.61-8.63 (m, 1H) MS (EI) m/z 413 (M+).
Table 27 (Compounds 37-2 and 38-2 are compounds of the invention; Compounds 37-1 and 38-1 are Reference Examples)
37-1

MS (EI) m/z 414 (M+).
37-2

1H NMR (δ, CDCl3): 3.85 (s, 3H), 5.24 (s, 2H), 6.56-6.59 (m, 2H), 6.66 (s, 1H), 6.97 (d, 1H, J=16.5 Hz), 7.13-7.18 (m, 2H), 7.22-7.25 (m, 1H), 7.30 (d, 1H, J=16.5 Hz), 7.37-7.40 (m, 1H), 7.43 (d, 1H, J=8.3 Hz), 7.51-7.53 (m, 1H), 7.56 (d, 1H, J=16.1 Hz), 7.63-7.67 (m, 1H), 7.70-7.74 (m, 1H), 8.59-8.62 (m, 2H) MS (EI) m/z 410 (M+).
38-1

MS (EI) m/z 464 (M+).
38-2

1H NMR (δ, Acetone-d6) : 3.92 (s, 3H), 5.24 (s, 2H), 6.67 (dd, 1H, J=2.6, 8.4 Hz), 6.76 (d, 1H, J=2.6 Hz), 6.80 (s, 1H), 7.09 (d, 1H, J=16. 7 Hz), 7.30-7.33 (m, 1H), 7.37-7.49 (m, 4H), 7.54 (d, 1H, J=9.0Hz), 7.66-7.68 (d, 1H, J=8.4 Hz), 7.80-7.84 (m, 1H), 7.90-7.95 (m, 2H), 8.09-8.11 (m, 1H), 8.26-8.29 (m, 1H), 8.58-8.60 (m, 1H), 8.89 (dd, 1H, J=1.9, 3.9 Hz) MS (EI) m/z 460 (M+).
Table 28 (Compounds 39-2 and 40-2 are compounds of the invention; Compounds 39-1 and 40-1 are Reference Examples)
39-1

1H NMR (δ, CDCl3) : 3.87 (s, 3H), 5.25 (s, 2H), 5.78 (s, 1H), 6.46-6.48 (m, 1H), 6.51 (d, 1H, J=15. 4 Hz), 6.57-6.61 (m, 3H), 6.62 (d, 1H, J=15. 4 Hz), 7.22-7.26 (m, 1H), 7.39 (d, 1H, J=15.4 Hz), 7.45-7.49 (m, 2H), 7.50 (d, 1H, J=7.7 Hz), 7.70-7.74 (m, 1H), 7.90 (d, 1H, J=15. 4 Hz), 8.61 (d, 1H, J=4.5 Hz) MS (EI) m/z 403 (M+).
39-2

1H NMR (δ, CDCl3) : 3.85 (s, 3H), 5.24 (s, 2H), 6.37-6.39 (m, 1H), 6. 41-6. 42 (m, 1H), 6.55-6.59 (m, 3H), 6.93-6.98 (m, 3H), 7.23-7.26 (m, 1H), 7.32 (d, 1H, J=16. 6 Hz), 7.39 (d, 1H, J=1.6 Hz), 7.42 (d, 1H, J=8.4 Hz), 7.53 (d, 1H, J=7.9 Hz), 7.71-7.75 (m, 1H), 8.60-8.62 (m, 1H) MS (EI) m/z 399 (M+).
40-1

1H NMR (δ, CDCl3) : 2.36 (s, 3H), 3.86 (s, 3H), 5.24 (s, 2H), 5.75 (s, 1H), 6.07-6.09 (m, 1H), 6.43 (d, 1H, J=15.4 Hz), 6.49-6.50 (m, 1H), 6.57-6.62 (m, 3H), 7.22-7.26 (m, 1H), 7.33 (d, 1H, J=15.4 Hz), 7.45-7.48 (m, 1H), 7.50 (d, 1H, J=7.7 Hz), 7.70-7.74 (m, 1H), 7.88 (d, 1H, J=16.0 Hz), 8.60-8.62 (m, 1H), 16.02 (brs, 1H) MS (EI) m/z 417 (M+).
40-2

1H NMR (δ, CDCl3) : 2.33 (s, 3H), 3.85 (s, 3H), 5.23 (s, 2H), 5.99-6.01 (m, 1H), 6.24 (d, 1H, J=3. 15 Hz), 6.53 (s, 1H), 6. 56-6. 59 (m, 2H), 6.79 (d, 1H, J=16.2 Hz), 6.87 (d, 1H, J=16.2 Hz), 6.95 (d, 1H, J=16.6 Hz), 7.21-7.25 (m, 1H), 7.28 (d, 1H, J=16.6 Hz), 7.43 (d, 1H, J=8.3 Hz), 7.52 (d, 1H, J=7.9 Hz), 7.70-7.74 (m, 1H), 8.60-8.62 (m, 1H) MS (EI) m/z 413 (M+).
Table 29 (Compounds 41-2 and 42-2 are compounds of the invention; Compounds 41-1 and 42-1 are Reference Examples)
41-1

1H NMR (δ, CDCl3): 1.95 (s, 3H), 2.27 (s, 3H), 3.86 (s, 3H), 5.25 (s, 2H), 5.74 (s, 1H), 6.38-6.42 (m, 2H), 6.57-6.62 (m, 3H), 7.23-7.31 (m, 2H), 7.47 (d, 1H, J=9.3Hz), 7.49-7.53 (m, 1H), 7.71-7.75 (m, 1H), 7.88 (d, 1H, J=16.3 Hz), 8.59-8.63 (m, 1H) MS (EI) m/z 431 (M+).
41-2

1H NMR (δ, CDCl3,): 1.94 (s, 3H), 2.24 (s, 3H), 3.85 (s, 3H), 5.23 (s, 2H), 6.16 (s, 1H), 6.53-6.59 (m, 3H), 6.78 (d, 1H, J=16. 4 Hz), 6.83 (d, 1H, J=16. 4 Hz), 6.96 (d, 1H, J=16. 8 Hz), 7.22-7.26 (m, 1H), 7.30 (d, 1H, J=16. 8 Hz), 7.43 (d, 1H, J=8.5 Hz), 7.51-7.53 (m, 1H), 7.70-7.74 (m, 1H), 8.60-8.62 (m, 1H) MS (EI) m/z 427 (M+).
42-1

MS (EI) m/z 417 (M+).
42-2

1H NMR (δ, CDC3): 3.85 (s, 3H), 3.95 (s, 3H), 5.24 (s, 2H), 6.47 (d, 1H, J=2.0 Hz), 6.57 (dd, 1H, J=2.4, 8.6 Hz), 6.59 (d, 1H, J=2.4 Hz), 6.60 (s, 1H), 6.93 (d, 1H, J=16.7 Hz), 6.97 (d, 1H, J=16.3 Hz), 7.03 (d, 1H, J=16. 3 Hz), 7. 23-7. 27 (m, 1H), 7.31 (d, 1H, J=16. 7 Hz), 7.41 (d, 1H, J=8. 3 Hz), 7.44 (d, 1H, J=2.0 Hz), 7.51-7.54 (m, 1H), 7.71-7.75 (m, 1H), 8.61-8.63 (m, 1H) MS (EI) m/z 413 (M+).
Table 30 (Compounds 43-2 and 44-2 are compounds of the invention; Compounds 43-1 and 44-1 are Reference Examples)
43-1

1H NMR (δ, CDCl3): 2.00-2.06 (m, 2H), 2.39 (s, 3H), 2.55-2.59 (m, 2H), 2.71-2.74 (m, 2H), 3.53-3.56 (m, 2H), 3.61-3.64 (m, 2H), 3.83 (s, 3H), 5.79 (s, 1H), 6.43 (d, 1H, J=15. 5 Hz), 6. 48-6. 50 (m, 1H), 6.65 (d, 1H, J=15.8 Hz), 6.67-6.70 (m, 2H), 7.12 (d, 1H, J=3.1 Hz), 7.37-7.40 (m, 1H), 7.43-7.49 (m, 3H), 7.59-7.64 (m, 2H), 7.80 (d, 1H, J=15.7 Hz) MS (EI) m/z 441 (M+).
43-2

1H NMR (δ, CDCl3) : 2.01-2.07 (m, 2H), 2.39 (s, 3H), 2.56-2.61 (m, 2H), 2.71-2.75 (m, 2H), 3.51-3.54 (m, 2H), 3.60-3.63 (m, 2H), 3.81 (s, 3H), 6.46-6.48 (m, 1H), 6.59 (s, 1H), 6.66-6.69 (m, 2H), 6.82 (d, 1H, J=16.5 Hz), 7.00 (d, 1H, J=16.4 Hz), 7.05-7.10 (m, 2H), 7.23 (d, 1H, J=16.4 Hz), 7.31-7.34 (m, 1H), 7.36-7.39 (m, 2H), 7.41-7.43 (m, 1H), 7.59 (d, 1H, J=8. 1 Hz) MS (EI) m/z 437 (M+).
44-1

MS (EI) m/z 480 (M+).
44-2

1 H-NMR (CDCl3) : δ 3.28 (t, 2H, J=6.7 Hz), 3.79 (s, 3H), 3.84 (s, 3H), 4.40 (t, 2H, J=6. 7 Hz), 6.45-6.47 (m, 2H), 6.51 (dd, 1H, J=2.3Hz, 8.3 Hz), 6. 62 (s, 1H), 6.96 (d, 1H, J=16.5 Hz), 7.05 (d, 1H, J=3.0 Hz), 7.08 (d, 1H, J=16. 5 Hz), 7.14-7.18 (m, 1H), 7.23 (d, 1H, J=16. 5 Hz), 7.26-7.29 (m, 1H), 7.29-7.33 (m, 2H), 7.40 (brs, 1H), 7.42 (d, 1H, J=8.6 Hz), 7.58 (d, 1H, J=8.3 Hz), 7.61-7.65 (m, 1H), 8.56-8.58 (m, 1H) MS (EI) m/z 476 (M+).
Table 31 (Compounds 45-2 and 46-2 are compounds of the invention; Compounds 45-1 and 46-1 are Reference Examples)
45-1

1H NMR (δ, CDCl3) : 3.82 (s, 3H), 3.86 (s, 6H), 5.25 (s, 2H), 5.82 (s, 1H), 6.24 (s, 2H), 6.48 (d, 1H, J=3.2 Hz), 6.66 (d, 1H, J=15.4 Hz), 7.01 (d, 1H, J=16.0 Hz), 7.11 (d, 1H, J=3.2 Hz), 7.23-7.26 (m, 1H), 7.37 (d, 1H, J=1.3 Hz, 8.4 Hz), 7.47 (s, 1H), 7.52 (d, 1H, J=7.7 Hz), 7.60 (d, 1H, J=8.4Hz), 7.71-7.75 (m, 1H), 7.79 (d, 1H, J=15.4Hz), 8.07 (d, 1H, J=16.0 Hz), 8.60-8.62 (m, 1H), 16.23 (brs, 1H) MS (EI) m/z 496 (M+).
45-2

1H NMR (δ, CDCl3) : 3.79 (s, 3H), 3.85 (s, 6H), 5.24 (s, 2H), 6.26 (s, 2H), 6.45 (d, 1H, J=3.2 Hz), 6.63 (s, 1H), 7.04 (d, 1H, J=2.75 Hz), 7.11 (d, 1H, J=16.1 Hz), 7.22-7.27 (m, 2H), 7.31 (dd, 1H, J=1.4 Hz, 8.3 Hz), 7.37 (s, 2H), 7.41 (s, 1H), 7.53 (d, 1H, J=7.75 Hz), 7.57 (d, 1H, J=8.3 Hz), 7.71-7.75 (m, 1H), 8.60-8.62 (m, 1H) MS (EI) m/z 492 (M+).
46-1

MS (EI) m/z 454 (M+).
46-2

MS (EI) m/z 450 (M+).
Table 32 (Compounds 47-2 and 48-2 are compounds of the invention; Compounds 47-1 and 48-1 are Reference Examples)
47-1

1H NMR (δ, CDCl3): 2.44-2.48 (m, 4H), 3.52 (s, 2H), 3.70-3.74 (m, 4H), 5.84 (s, 1H), 6.57-6.59 (m, 1H), 6.62 (d, 1H, J=15. 9 Hz), 6.66 (d, 1H, J=15.8 Hz), 7.29-7.31 (m, 1H), 7.35-7.38 (m, 2H), 7.39-7.42 (m, 1H), 7.50-7.53 (m, 2H), 7.57-7.58 (m, 1H), 7.63-7.67 (m, 2H), 7.81 (d, 1H, J=15.7 Hz), 8.31 (brs, 1H), 16.03 (brs, 1H) MS (EI) m/z 414 (M+).
47-2

1H NMR (δ, CDCl3): 2.44-2.48 (m, 4H), 3.51 (s, 2H), 3.70-3.74 (m, 4H), 6.54-6.55 (m, 1H), 6.63 (s, 1H), 7.03 (d, 1H, J=16.3 Hz), 7.04 (d, 1H, J=16.5 Hz), 7.09 (d, 1H, J=16.5 Hz), 7.20 (d, 1H, J=16.2 Hz), 7.21-7.23 (m, 1H), 7.30-7.34 (m, 3H), 7.44-7.47 (m, 3H), 7.61 (d, 1H, J=8.3 Hz), 8.16 (brs, 1H) MS (EI) m/z 410 (M+).
48-1

1H NMR (δ, CDCl3): 2.49-2.56 (m, 4H), 2.59-2.64 (m, 2H), 2.81-2.86 (m, 2H), 3. 72-3. 76 (m, 4H), 5.83 (s, 1H), 6. 56-6. 58 (m, 1H), 6.59 (d, 1H, J=16.0 Hz), 6.65 (d, 1H, J=16.0 Hz), 7.24 (d, 2H, J=8.2 Hz), 7.28-7.30 (m, 1H), 7.39-7.41 (m, 1H), 7.48 (d, 2H, J=8.2 Hz), 7.57 (s, 1H), 7.61-7.65 (m, 2H), 7.80 (d, 1H, J=16.0 Hz), 8.30 (brs, 1H), 16.00 (brs, 1H) MS (EI) m/z 428 (M+).
48-2

1H NMR (δ, DMSO-d6) : 2.41-2.44 (m, 4H), 2.49-2.55 (m, 2H), 2.74 (t, 2H, J=7.7Hz), 3.56-3.60 (m, 4H), 6.42 (brs, 1H), 6.73 (s, 1H), 6.96-7.39 (m, 8H), 7.43-7.56 (m, 4H), 11.04-11.17 (m, 1H), 12.88 (s, 1H) MS (EI) m/z 424 (M+).
Table 33 (Compounds 49-2 and 50-2 are compounds of the invention; Compounds 49-1 and 50-1 are Reference Examples)
49-1

1H NMR (δ, CDCl3): 1.81-1.88 (m, 2H), 2.35-2.51 (m, 6H), 2.68 (t, 2H, J=7.7 Hz), 3.70-3.77 (m, 4H), 5.83 (s, 1H), 6.57-6.62 (m, 2H), 6.66 (d, 1H, J=15.9Hz), 7.20-7.23 (m, 2H), 7.30-7.31 (m, 1H), 7.41 (dd, 1H, J=1.4, 8.2 Hz), 7.47-7.50 (m, 2H), 7.57-7.58 (m, 1H), 7.62-7.66 (m, 2H), 7.81 (d, 1H, J=15.9 Hz), 8.32 (brs, 1H) MS (EI) m/z 442 (M+).
49-2

1H NMR (δ, CDCl3): 1.81-1.88 (m, 2H), 2.39 (t, 2H, J=7.6Hz), 2.44-2.49 (m, 4H), 2.65 (t, 2H, J=7.7 Hz), 3.72-3.76 (m, 4H), 6.54-6.56 (m, 1H), 6.64 (s, 1H), 7.00-7.06 (m, 2H), 7.08 (d, 1H, J=16.6 Hz), 7.16-7.23 (m, 4H), 7.33 (dd, 1H, J=1.2, 8.5 Hz), 7.40-7.43 (m, 2H), 7.47-7.48 (m, 1H), 7.61 (d, 1H, J=8.3 Hz), 8.19 (brs, 1H) MS (EI) m/z 438 (M+).
50-1

1H NMR (δ, CDCl3): 2.29 (s, 3H), 2.35-2.61 (m, 8H), 3.53 (s, 2H), 5.84 (s, 1H), 6.57-6.59 (m, 1H), 6.61 (d, 1H, J=15.9 Hz), 6.66 (d, 1H, J=15.8 Hz), 7.29-7.31 (m, 1H), 7.34-7.37 (m, 2H), 7.40-7.42 (m, 1H), 7.49-7.52 (m, 2H), 7.56-7.58 (m, 1H), 7.64 (d, 1H, J=8.3 Hz), 7.65 (d, 1H, J=15.9 Hz), 7.81 (d, 1H, J=15.7 Hz), 8.33 (brs, 1H), 16.03 (brs, 1H) MS (EI) m/z 427 (M+).
50-2

1H NMR (δ, CDCl3): 2.29 (s, 3H), 2.40-2.57 (m, 8H), 3.51 (s, 2H), 6.54-6.56 (m, 1H), 6.63 (s, 1H), 7.02 (d, 1H, J=16.4Hz), 7.03 (d, 1H, J=16. 5 Hz), 7.08 (d, 1H, J=16.5 Hz), 7.19 (d, 1H, J=16.5 Hz), 7.20-7.23 (m, 1H), 7.29-7.34 (m, 3H), 7.42-7.48 (m, 3H), 7.61 (d, 1H, J=8.3 Hz), 8.23 (brs, 1H) MS (EI) m/z 423 (M+).
Table 34 (Compounds 51-2 and 52-2 are compounds of the invention; Compounds 51-1 and 52-1 are Reference Examples)
51-1

1H NMR (δ, CDCl3): 2.30 (s, 3H), 2.40-2.65 (m, 10H), 2.80-2.85 (m, 2H), 5.83 (s, 1H), 6.56-6.61 (m, 2H), 6.65 (d, 1H, J=15.9 Hz), 7.23 (d, 2H, J=8.1 Hz), 7.28-7.30 (m, 1H), 7.40 (dd, 1H, J=1.5 Hz, 8.05 Hz), 7.47 (d, 2H, J=8.1 Hz), 7.56 (s, 1H), 7.61-7.65 (m, 2H), 7.80 (d, 1H, J=15. 9 Hz), 8.34 (brs, 1H), 15.96 (brs, 1H) MS (EI) m/z 441 (M+).
51-2

1H NMR (δ, CDCl3): 2.32 (s, 3H), 2.41-2.69 (m, 10H), 2.78-2.85 (m, 2H), 6.54-6.56 (m, 1H), 6.63 (s, 1H), 7.01 (d, 1H, J=16.5 Hz), 7.03 (d, 1H, J=16. 4 Hz), 7.07 (d, 1H, J=16.5Hz), 7.16-7.22 (m, 3H), 7.22-7.24 (m, 1H), 7.33 (dd, 1H, J=1.3 Hz, 8.2Hz), 7.42 (d, 2H, J=8.1 Hz), 7.48 (brs, 1H), 7.62 (d, 1H, J=8.2 Hz), 8.19 (brs, 1H) MS (EI) m/z 437 (M+).
52-1

1H NMR (δ, CDCl3): 2.63 (t, 4H, J=4.8 Hz), 2.89 (t, 2H, J=5.8 Hz), 3.76 (t, 4H, J=4.8 Hz), 4.20 (t, 2H, J=5.8 Hz), 5.83 (s, 1H), 6.56-6.58 (m, 1H), 6.65 (d, 1H, J=16. 0 Hz), 6.76 (d, 1H, J=16. 0 Hz), 6.93 (d, 1H, J=8. 0 Hz), 6.96-7.00 (m, 1H), 7.28-7.34 (m, 2H), 7.40 (dd, 1H, J=1.5 Hz, 8.0 Hz), 7.54-7.58 (m, 2H), 7.64 (d, 1H, J=8. 25 Hz), 7.80 (d, 1H, J=16. 0 Hz), 7.97 (d, 1H, J=16.0 Hz), 8.31 (brs, 1H), 16.01 (brs, 1H) MS (EI) m/z 444 (M+).
52-2

1H NMR (δ, CDCl3): 2.62 (t, 4H, J=4.6 Hz), 2.88 (t, 2H, J=5.8 Hz), 3.75 (t, 4H, J=4.6 Hz), 4.18 (t, 2H, J=5.8 Hz), 6.52-6.54 (m, 1H), 6.61 (s, 1H), 6.90 (d, 1H, J=7. 7 Hz), 6.94-6.98 (m, 1H), 7.06 (d, 1H, J=16. 3 Hz), 7.13 (d, 1H, J=16. 7 Hz), 7.18-7.25 (m, 3H), 7.31-7.34 (m, 1H), 7.42-7.47 (m, 2H), 7.55 (dd, 1H, J=1.6 Hz, 7.7 Hz), 7.60 (d, 1H, J=8.2 Hz), 8.15 (brs, 1H) MS (EI) m/z 440 (M+).
Table 35 (Compounds 53-2 and 54-2 are compounds of the invention; Compounds 53-1 and 54-1 are Reference Examples)
53-1

1H NMR (δ, CDCl3): 2.56-2.62 (m, 4H), 2.81-2.84 (m, 2H), 3.72-3.76 (m, 4H), 4. 13-4. 17 (m, 2H), 5.84 (s, 1H), 6.56-6.59 (m, 1H), 6.60 (d, 1H, J=15. 9 Hz), 6.65 (d, 1H, J=15. 9 Hz), 6.92 (dd, 1H, J=2. 45 Hz, 8.1 Hz), 7.08-7.10 (m, 1H), 7.15 (d, 1H, J=7.85 Hz), 7.27-7.32 (m, 2H), 7.39-7.42 (m, 1H), 7.57 (s, 1H), 7.60 (d, 1H, J=15.9 Hz), 7.64 (d, 1H, J=8.1 Hz), 7.81 (d, 1H, J=15.9 Hz), 8.32 (brs, 1H), 15.97 (brs, 1H) MS (EI) m/z 444 (M+).
53-2

1H NMR (δ, CDCl3): 2.68 (brs, 4H), 2.90 (brs, 2H), 3.79 (brs, 4H), 4.21 (brs, 2H), 6.54-6.56 (m, 1H), 6.63 (s, 1H), 6.82-6.85 (m, 1H), 7.02 (d, 1H, J=16.3 Hz), 7.04-7.06 (m, 3H), 7.10 (d, 1H, J=7.7 Hz), 7.19 (d, 1H, J=16.3 Hz), 7.22-7.24 (m, 1H), 7.25-7.29 (m, 1H), 7.33 (dd, 1H, J=1.4 Hz, 8.3 Hz), 7.48 (s, 1H), 7.62 (d, 1H, J=8.3Hz), 8.19 (brs, 1H) MS (EI) m/z 440 (M+).
54-1

MS (EI) m/z 466 (M+).
54-2

1H NMR (δ, CDCl3): 3.29 (t, 2H, J=6.7 Hz), 3.86 (s, 3H), 4.41 (t, 2H, J=6. 7 Hz), 6.47 (d, 1H, J=2. 3 Hz), 6. 52-6. 56 (m, 2H), 6.62 (s, 1H), 6.96 (d, 1H, J=16. 5 Hz), 7.05 (d, 1H, J=16. 5 Hz), 7.15-7.23 (m, 3H), 7.24-7.28 (m, 1H), 7.30 (d, 1H, J=16.5 Hz), 7.33 (dd, 1H, J=1.4Hz, 8.3 Hz), 7.43 (d, 1H, J=8.3 Hz), 7.49 (s, 1H), 7.61 (d, 1H, J=8.7 Hz), 7.61-7.66 (m, 1H), 8.17 (brs, 1H), 8.56-8.59 (m, 1H) MS (EI) m/z 462 (M+).
Table 36 (Compounds 55-2 and 56-2 are compounds of the invention; Compounds 55-1 and 56-1 are Reference Examples)
55-1

1H NMR (δ, CDCl3): 2.15 (s, 3H), 4.25-4.30 (m, 4H), 5.61 (s, 1H), 6.31 (d, 1H, J=15.7 Hz), 6.86 (d, 1H, J=8.3 Hz), 7.02 (dd, 1H, J=2.0Hz, 8.3 Hz), 7.05 (d, 1H, J=2.0 Hz), 7.48 (d, 1H, J=15.7 Hz), 15.41 (brs, 1H)
55-2

MS (EI) m/z 493 (M+).
55-3

1H NMR (δ, CDCl3): 2.58-2.61 (m, 4H), 2.81 (t, 2H, J=5. 7 Hz), 3. 73-3. 76 (m, 4H), 3.86 (s, 3H), 4.14 (t, 2H, J=5.7 Hz), 4.26-4.27 (m, 4H), 6.48-6.51 (m, 2H), 6.58 (s, 1H), 6.84 (d, 1H, J=8.3 Hz), 6.89 (d, 1H, J=16. 5 Hz), 6.93-6.99 (m, 3H), 7.01 (d, 1H, J=1.8Hz), 7.29 (d, 1H, J=16.5 Hz), 7.43 (d, 1H, J=8.7 Hz) MS (EI) m/z 489 (M+).
56-1

1H NMR (δ, CDCl3): 2. 56-2. 60 (m, 8H), 2.81 (t, 4H, J=5. 7 Hz), 3. 72-3. 75 (m, 8H), 3.88 (s, 6H), 4.15 (t, 4H, J=5.7 Hz), 5.79 (s, 1H), 6.48 (d, 2H, J=2.5 Hz), 6.51 (dd, 2H, J=2.5Hz, 8.5 Hz), 6.62 (d, 2H, J=16.0 Hz), 7.47 (d, 2H, J=8.5 Hz), 7.77 (d, 2H, J=16.0 Hz), 16.13 (brs, 1H), MS (EI) m/z 594 (M+).
56-2

1H NMR (δ, CDCl3): 2.57-2.61 (m, 8H), 2.81 (t, 4H, J=5.7 Hz), 3.73-3.76 (m, 8H), 3.86 (s, 6H), 4.14 (t, 4H, J=5.7 Hz), 6.47-6.52 (m, 4H), 6.61 (s, 1H), 6.97 (d, 2H, J=16.5 Hz), 7.30 (d, 2H, J=16.5 Hz), 7.44 (d, 2H, J=8. 3 Hz) MS (EI) m/z 590 (M+).
Table 37 (Compounds 57-2 and 57-2 are compounds of the invention; Compounds 58-1 and 58-1 are Reference Examples)
57-1

MS (EI) m/z 436 (M+).
57-2

1H NMR (δ, CDCl3): 3.81 (s, 3H), 5.23 (s, 2H), 6.62 (s, 1H), 6.80 (s, 1H), 6.89 (d, 1H, J=16.4 Hz), 6.97 (d, 2H, J=8.7 Hz), 7.04 (d, 1H, J=16.4 Hz), 7.07-7.12 (m, 1H), 7.10 (d, 1H, J=16. 3 Hz), 7.17 (d, 1H, J=16. 3 Hz), 7.18-7.30 (m, 3H), 7.41 (d, 2H, J=8.7 Hz), 7.52 (d, 1H, J=7.9 Hz), 7.58 (d, 1H, J=7.8 Hz), 7.70-7.74 (m, 1H), 8.61 (d, 1H, J=4.4 Hz) MS (EI) m/z 432 (M+).
58-1

MS (EI) m/z 466 (M+).
58-2

1H NMR (δ, Acetone-d6): 3.84 (s, 3H), 3.91 (s, 3H), 5.23 (s, 2H), 6.44-6.46 (m, 1H), 6.65-6.68 (m, 2H), 6.74 (d, 1H, J=2.3 Hz), 7.04-7.09 (m, 2H), 7.21 (d, 1H, J=3.2 Hz), 7.29-7.34 (m, 2H), 7.36-7.41 (m, 2H), 7.46 (dd, 1H, J=1.4, 8.7 Hz), 7.53 (d, 1H, J=8.3 Hz), 7.56-7.58 (m, 1H), 7.70-7.72 (m, 1H), 7.80-7.84 (m, 1H), 8.58-8.60 (m, 1H), 11.93 (brs, 1H) MS (EI) m/z 462 (M+).
Table 38 (Compound 59-2 is a compound of the invention; Compound 59-1 is a Reference Example)
59-1

1H NMR (δ, CDCl3): 2.45 (s, 3H), 3.83 (s, 3H), 5.23 (s, 2H), 5.82 (s, 1H), 6. 45-6. 50 (m, 2H), 6.67 (d, 1H, J=15. 8 Hz), 6. 84-6. 87 (m, 2H), 7.13 (d, 1H, J=3.0 Hz), 7.22-7.27 (m, 1H), 7.37-7.40 (m, 1H), 7.48-7.52 (m, 2H), 7.56-7.63 (m, 2H), 7.70-7.74 (m, 1H), 7.83 (d, 1H, J=15. 8 Hz), 7.89 (d, 1H, J=15. 8 Hz), 8. 60-8. 62 (m, 1H) MS (EI) m/z 450 (M+).
59-2

1H NMR (δ, CDCl3): 2.41 (s, 3H), 3.80 (s, 3H), 5.22 (s, 2H), 6.46-6.48 (m, 1H), 6.63 (s, 1H), 6.82-6.87 (m, 3H), 7.06 (d, 1H, J=3.1 Hz), 7.07 (d, 1H, J=16.5 Hz), 7.21-7.28 (m, 3H), 7.31-7.33 (m, 1H), 7.40-7.42 (m, 1H), 7.50-7.54 (m, 2H), 7.59 (d, 1H, J=8.2 Hz), 7.70-7.74 (m, 1H), 8.60-8.62 (m, 1H) MS (EI) m/z 446 (M+).

Pharmacological test example 1: Determination of tau aggregation inhibitory activity



[0208] Recombinant three-repeat microtubule-binding domain (3R-MBD) of tau protein was expressed in E. coli and was purified and used for the experiment. The purified tau solution was diluted with a 50 mM Tris-HCl buffer (pH 7.6) to a final concentration of 10 µM. The test compounds were prepared using dimethylsulfoxide (hereinafter also referred to as DMSO) at 20-fold of their final concentrations and added to the tau solution so that the DMSO concentration would be 5%. To this solution, heparin was added so that the final concentration would be 10 µM and the plate was left to stand at 37°C for 16 hours. Thioflavin T was added to the plate so that the concentration would be 10 µM and the fluorescence intensity was measured with a fluorescence plate reader (PerkinElmer, Inc.)(excitation wavelength: 440 nm; fluorescence wavelength: 486 nm).

[0209] The final concentration of each compound at the time of measurement was set at 0.1, 0.3, 1, 3, and 10 µM. The sample to which only DMSO was added was used as a negative control and its fluorescence intensity was taken as 0% inhibitory activity to determine the 50% inhibitory concentration (IC50) of each compound. The results are shown in Table 39. Table 39 also shows the calculated maximum inhibition (%) of the compounds that exhibited particularly high maximum inhibition (%) at the concentrations used for the determination of the tau aggregation inhibitory activity.
Table 39
Compound of the inventionIC50 (µM)Maximum inhibition (%)
Example 16-2 0.6 -
Example 17-2 0.65 -
Example 19-4 0.51 -
Example 20-3 0.55 89
Example 21-2 - 89
Example 22-2 0.66 90
Example 23-2 0.59 92
Example 25-2 0.84 -
Example 27-3 - 91
Example 28-2 - 93
Example 32-5 0.64 90
Example 42-2 0.83 -
Example 43-2 - 92
Example 53-2 0.84 -


[0210] As is apparent from Table 39, some of the compounds of the present invention exhibited a particularly high tau aggregation inhibitory activity. The compounds that exhibited high maximum inhibition are expected to have improved therapeutic effects and are thus more preferred.

Pharmacological test example 2: Determination of β-secretase inhibitory activity



[0211] The β-secretase inhibitory activity was measured with BACE-1 FRET assay Kit (Invitrogen). The test compounds were prepared using DMSO at 30-fold of their final concentrations and added to an assay buffer so that the DMSO concentration would be 10%. To each of the solutions, equal volumes of a human recombinant β-secretase (1 U/mL) and a fluorescent substrate peptide (750 nM), each dissolved in an assay buffer, were added and the solutions were left to stand for 1 hour. The fluorescence intensity was measured with a fluorescence plate reader (excitation wavelength: 545 nm; fluorescence wavelength: 590 nm).

[0212] The final concentration of each compound at the time of measurement was set at 0.1, 0.3, 1, 3, and 10 µM, at 0.3, 1, 3, 10, and 30 µM, or at 1, 3, 10, 30, and 100 µM. The sample to which only DMSO was added was used as a negative control and its fluorescence intensity was taken as 0% inhibitory activity to determine the 50% inhibitory concentration (IC50) of each compound for the evaluation of β-secretase inhibitory activity of each compound. The fluorescent substrate peptide had the amino acid sequence of Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Lys-Arg, in which the Ser residue at position 1 was labeled with a fluorescent donor (Cy3) and the Lys at position 9 was labeled with a fluorescence quencher (Cy5Q).

[0213] The compounds of the present invention exhibited a high β-secretase inhibitory activity.

Pharmacological test example 3: Determination of Aβ aggregation inhibitory activity



[0214] Aβ 1-42 (Peptide Institute, Inc.) was dissolved in 0.1% ammonia water so that the concentration would be 0.5 mM, and then diluted with PBS to 20 µM. The test compounds were prepared using DMSO at 100-fold of their final concentrations and then diluted with PBS so that the DMSO concentration would be 2%. The Aβ solution and each of the test compound solutions were mixed at an equivalent ratio and the mixtures were left to stand at 37°C for 24 hours. To each of the mixtures, an equal volume of a thioflavin T solution adjusted with a 100 mM Tris-glycine buffer (pH 8.5) to 6 µM was added. The fluorescence intensity was measured with a fluorescence plate reader (excitation wavelength: 440 nm; fluorescence wavelength: 486 nm).

[0215] The final concentration of each compound at the time of measurement was set at 0.1, 0.3, 1, 3, and 10 µM or at 2, 4, 8, and 16 µM. The sample to which only DMSO was added was used as a negative control and its fluorescence intensity was taken as 0% inhibitory activity. With the use of the fluorescence intensity of the control taken as 0% inhibitory activity, the 50% inhibitory concentration (IC50) of each compound was calculated to evaluate the Aβ aggregation inhibitory activity of each compound.

[0216] The compounds of the present invention exhibited a high Aβ aggregation inhibitory activity.

Pharmacological test example 4: Concentration measurement in brain after oral administration



[0217] Each of the compounds of the present invention shown in Tables 40 and 41 and the compound of Example 2 of JP 2012-229208 A was orally administered to ICR mice (male, 7 weeks old) in a single dose of 5 mg/kg. At 3, 6 and 9 hours after the administration, 1, 3, 6 and 9 hours after the administration, or 1, 3, 6, 9 and 24 hours after the administration, the mice were subjected to perfusion by infusing physiological saline into the heart under inhalational anesthesia with isoflurane, and the whole brain was harvested. Physiological saline was added to the harvested brain in an amount equal to twice the wet weight of the brain and the brain was homogenized. Then, an equal volume of methanol was added thereto, and the mixture was deproteinized. The concentration of each compound in the sample was measured by LC/MS/MS. The test results are shown in Tables 40 and 41.
Table 40
Compound of the inventionAUC0-9h in brain (ng·hr/g-tissue)
Example 22-2 458
Example 44-2 869
JP 2012-229208 A, Example 2 224
Table 41
Compound of the inventionAUC0-24h in brain (ng·hr/g-tissue)
Example 14-2 24639
Example 18-2 9781
Example 19-4 3499
Example 20-3 5306
Example 27-3 1302
JP 2012-229208 A, Example 2 645 to 677

Industrial Applicability



[0218] The compound of the present invention is useful as a prophylactic drug, a therapeutic drug, and the like for Alzheimer's disease and therefore the present invention can be applied to industrial fields such as pharmaceutical industry.


Claims

1. A compound represented by formula:

























































or

or a pharmaceutically acceptable salt thereof
 
2. The compound according to claim 1, represented by formula:





or

or a pharmaceutically acceptable salt thereof.
 
3. A pharmaceutical composition comprising the compound according to claim 1 or 2 or a salt thereof as an active ingredient.
 
4. The pharmaceutical composition according to claim 3 for use in the prevention or treatment of a disease selected from the group consisting of Alzheimer's disease, familial Alzheimer's disease, sporadic Alzheimer's disease, senile dementia, Down syndrome, Parkinson's disease, Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, diabetic neuropathy, Huntington's chorea, and multiple sclerosis.
 
5. The compound according to claim 1 or 2 or a salt thereof for use in the prevention or treatment of a disease selected from the group consisting of Alzheimer's disease, familial Alzheimer's disease, sporadic Alzheimer's disease, senile dementia, Down syndrome, Parkinson's disease, Creutzfeldt-Jakob disease, amyotrophic lateral sclerosis, diabetic neuropathy, Huntington's chorea, and multiple sclerosis.
 
6. The pharmaceutical composition for use according to claim 4 wherein the disease is Alzheimer's disease.
 
7. The compound according to claim 1 or 2 or a salt thereof for use in the prevention or treatment of Alzheimer's disease.
 
8. An oral or parenteral preparation comprising the compound according to claim 1 or 2 or a salt thereof and one or more pharmacologically acceptable carriers.
 


Ansprüche

1. Verbindung, dargestellt durch Formel:

























































oder

oder ein pharmazeutisch akzeptables Salz davon.
 
2. Verbindung nach Anspruch 1, dargestellt durch Formel:





oder

oder ein pharmazeutisch akzeptables Salz davon.
 
3. Pharmazeutische Zusammensetzung, umfassend die Verbindung nach Anspruch 1 oder 2 oder ein Salz davon als einen Wirkstoff.
 
4. Pharmazeutische Zusammensetzung nach Anspruch 3 zur Verwendung bei der Vorbeugung oder Behandlung einer Krankheit, ausgewählt aus der Gruppe, bestehend aus der Alzheimer-Krankheit, der familiären Alzheimer-Krankheit, sporadischer Alzheimer-Krankheit, seniler Demenz, dem Down-Syndrom, der Parkinson-Krankheit, der Creutzfeldt-Jakob-Krankheit, amyotropher Lateralsklerose, diabetischer Neuropathie, Huntington-Chorea und multipler Sklerose.
 
5. Verbindung nach Anspruch 1 oder 2 oder ein Salz davon zur Verwendung bei der Vorbeugung oder Behandlung einer Krankheit, ausgewählt aus der Gruppe, bestehend aus der Alzheimer-Krankheit, der familiären Alzheimer-Krankheit, sporadischer Alzheimer-Krankheit, seniler Demenz, dem Down-Syndrom, der Parkinson-Krankheit, der Creutzfeldt-Jakob-Krankheit, amyotropher Lateralsklerose, diabetischer Neuropathie, Huntington-Chorea und multipler Sklerose.
 
6. Pharmazeutische Zusammensetzung zur Verwendung nach Anspruch 4, wobei die Krankheit die Alzheimer-Krankheit ist.
 
7. Verbindung nach Anspruch 1 oder 2 oder ein Salz davon zur Verwendung bei der Vorbeugung oder Behandlung der Alzheimer-Krankheit.
 
8. Orales oder parenterales Präparat, umfassend die Verbindung nach Anspruch 1 oder 2 oder ein Salz davon und einen oder mehrere pharmakologisch akzeptable Träger.
 


Revendications

1. Composé représenté par la formule :

























































ou

ou un sel de celui-ci pharmaceutiquement acceptable.
 
2. Composé selon la revendication 1, représenté par la formule





ou

ou un sel de celui-ci pharmaceutiquement acceptable.
 
3. Composition pharmaceutique comprenant le composé selon la revendication 1 ou 2 ou un sel de celui-ci en tant que principe actif.
 
4. Composition pharmaceutique selon la revendication 3 pour utilisation dans la prévention ou le traitement d'une maladie choisie dans le groupe constitué de la maladie d'Alzheimer, la maladie d'Alzheimer familiale, la maladie d'Alzheimer sporadique, la démence sénile, le syndrome de Down, la maladie de Parkinson, la maladie de Creutzfeldt-Jakob, la sclérose latérale amyotrophique, la neuropathie diabétique, la chorée de Huntington et la sclérose en plaques.
 
5. Composé selon la revendication 1 ou 2 ou un sel de celui-ci pour utilisation dans la prévention ou le traitement d'une maladie choisie dans le groupe constitué de la maladie d'Alzheimer, la maladie d'Alzheimer familiale, la maladie d'Alzheimer sporadique, la démence sénile, le syndrome de Down, la maladie de Parkinson, la maladie de Creutzfeldt-Jakob, la sclérose latérale amyotrophique, la neuropathie diabétique, la chorée de Huntington et la sclérose en plaques.
 
6. Composition pharmaceutique pour utilisation selon la revendication 4, dans laquelle la maladie est la maladie d'Alzheimer.
 
7. Composé selon la revendication 1 ou 2 ou un sel de celui-ci pour utilisation dans la prévention ou le traitement de maladie d'Alzheimer.
 
8. Préparation orale ou parentérale comprenant le composé selon la revendication 1 ou 2 ou un sel de celui-ci et un ou plusieurs supports pharmacologiquement acceptables.
 






Cited references

REFERENCES CITED IN THE DESCRIPTION



This list of references cited by the applicant is for the reader's convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard.

Patent documents cited in the description




Non-patent literature cited in the description