(19)
(11)EP 2 941 418 B1

(12)EUROPEAN PATENT SPECIFICATION

(45)Mention of the grant of the patent:
29.04.2020 Bulletin 2020/18

(21)Application number: 14748929.8

(22)Date of filing:  06.02.2014
(51)International Patent Classification (IPC): 
C07C 279/26(2006.01)
A61K 31/155(2006.01)
C07D 211/14(2006.01)
C07D 211/70(2006.01)
C07C 277/02(2006.01)
C07D 207/20(2006.01)
C07D 211/16(2006.01)
(86)International application number:
PCT/KR2014/001006
(87)International publication number:
WO 2014/123364 (14.08.2014 Gazette  2014/33)

(54)

N1-CYCLIC AMINE-N5-SUBSTITUTED BIGUANIDE DERIVATIVES, METHODS OF PREPARING THE SAME AND PHARMACEUTICAL COMPOSITION COMPRISING THE SAME

N1-CYCLISCHE AMIN-N5-SUBSTITUIERTE BIGUANIDDERIVATE, VERFAHREN ZUR HERSTELLUNG DAVON UND PHARMAZEUTISCHE ZUSAMMENSETZUNG DAMIT

DÉRIVÉS DE BIGUANIDE À SUBSTITUTION N1-AMINE CYCLIQUE-N5, LEURS PROCÉDÉS DE PRÉPARATION ET COMPOSITION PHARMACEUTIQUE LES COMPRENANT


(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30)Priority: 07.02.2013 KR 20130014175
07.02.2013 KR 20130014176

(43)Date of publication of application:
11.11.2015 Bulletin 2015/46

(73)Proprietor: Immunomet Therapeutics Inc.
Houston, TX 77021 (US)

(72)Inventors:
  • MIN, Chang Hee
    Seoul 133-765 (KR)
  • KIM, Yong Eun
    Daejeon 301-819 (KR)
  • OH, Byung Kyu
    Gyeryong-si Chungcheongnam-do 321-900 (KR)
  • LEE, Ji Sun
    Daejeon 305-741 (KR)
  • HEO, Hye Jin
    Daejeon 305-509 (KR)
  • OH, Ju Hoon
    Gangneung-si Gangwon-do 210-932 (KR)
  • CHO, Woong
    Daejeon 305-509 (KR)

(74)Representative: Vossius & Partner Patentanwälte Rechtsanwälte mbB 
Siebertstrasse 3
81675 München
81675 München (DE)


(56)References cited: : 
WO-A1-92/07560
GB-A- 599 714
US-A- 2 467 371
US-A- 4 080 460
WO-A2-2013/022278
KR-A- 20110 081 093
US-A- 3 960 949
  
      
    Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).


    Description

    Technical Field



    [0001] The present invention relates to an N1-cyclic amine-N5-substituted biguanide derivative that inhibits cancer cell proliferation, cancer metastasis and cancer recurrence by activation of AMPK, even when administered in a small dose compared with conventional drugs, and that exhibits excellent therapeutic effects, a method of preparing the same, and a pharmaceutical composition including the N1-cyclic amine-N5-substituted biguanide derivative as an active ingredient.

    Background Art



    [0002] AMPK (AMP-activated protein kinases) is an enzyme that functions to regulate a metabolic pathway so as to maintain balance between supply of nutrients and demand for energy, and thus maintain energy homeostasis in cells and the whole body. AMPK is activated as a ratio of AMP/ATP in the cells increases due to a hypoxemic state or glucose deficiency. The activated AMPK induces fatty acid oxidation to produce a larger amount of ATP and inhibits anabolisms requiring the use of ATP. AMPK inhibits proliferation of cancer cells and kills the cancer cells by regulating energy metabolism in the cancer cells as well as in normal cells. AMPK activated in the cancer cells shows anticancer activities by phosphorylating mTORC1, p53, fatty acid synthase and the like to regulate the cell cycle, cell polarity, autophagy, apoptosis, etc.

    [0003] Metformin has been used to treat insulin-independent diabetes mellitus (i.e., type II diabetes mellitus) since, among oral therapeutic agents for treating diabetes mellitus, it is most effective at lowering blood glucose, does not cause hypoglycemia or hyperin-sulinemia and can prevent complications. In recent years, metformin has been extensively researched. Also, it was reported that metformin activates AMP-activated protein kinase (AMPK) by inhibiting the action of complex 1 of the electron transport system in mitochondria to obstruct intracellular generation of energy, and inhibits activation of the mTOR/S6K1 signaling pathway in which proteins essential for survival are produced to obstruct proliferation of cancer cells and tumor growth (Mol. Cancer Ther. 9(5): 1092-1099 (2010)). Consequently, metformin has received considerable attention as an anticancer agent for regulating cancer cell metabolism. Also, an epidemiological survey confirmed that the incidence of cancer and mortality by cancer were lower for patients treated with metformin (BMJ.330: 1304-1305 (2005)).

    [0004] Meanwhile, there is increasing clinical evidence indicating that cancer stem cells take part in recurrence and metastasis of cancer. The cancer stem cells refer to cancer cells that have self-regeneration or differentiation activities which are characteristically innate to stem cells. The cancer stem cells are present in the cancer tissues at a content of 0.2% or less, and are characterized by their slow proliferation. Since many anticancer agents developed so far target cancer cells that proliferate rapidly, the cancer stem cells are resistant to conventional anticancer therapy when cancer stem cells are treated with the anticancer agents, thereby causing poor prognoses. On the other hand, it was reported that metformin prevents the recurrence of cancer as it selectively acts only on cancer stem cells among breast cancer cells and removes the cancer stem cells (Cancer Res. 69(19): 7507-11 (2009)). Also, it was found that metformin prevents the metastasis of cancer by interfering with the motility and invasion of the cancer since it inhibits the expression of Snail1, Slug, Twist, ZEB1/2 and TGF-b, which are transcription factors associated with the epithelial-to-mesenchymal transition (EMT) and promotes the expression of E-cadherin to prevent cancer cells from leading to the EMT (Cell Cycle 10: 7, 1144-1151 (2011), Cell Cycle 9: 18, 3807-3814 (2010), Cell Cycle 9: 22, 4461-4468 (2010)).

    [0005] For these reasons, there is need for a biguanide-based substance that exhibits better pharmacological action than conventional metformin and has improved physio-chemical properties.

    [0006] GB-A-599,714 discloses biguanidine derivatives and their application as antimalarial agents.

    Disclosure of Invention


    Technical Problem



    [0007] The present invention is directed to providing a novel biguanide derivative that is highly effective at inhibiting proliferation of cancer cells, cancer metastasis and cancer recurrence, even when administered in a small dose compared with conventional drugs, or a pharmaceutically acceptable salt thereof, and a method of preparing the same.

    Solution to Problem



    [0008] One aspect of the present invention provides an N1-cyclic amine-N5-substituted biguanide derivative compound represented by the following Formula 1, or a pharmaceutically acceptable salt thereof:



    [0009] In Formula 1, R1 and R2 are taken together with nitrogen to which they are attached to form C3-6 heterocycloalkene having 4 to 7 ring atoms; or piperidinyl, which is substituted with C1-4 alkyl in at least one position thereof; and
    R3 is hydrogen; C1-6 alkyl; phenyl; or C1-4 alkyl substituted with phenyl, wherein the C3-6 heterocycloalkene is independently unsubstituted or substituted with at least one substituent selected from the group consisting of halogen, hydroxy and C1-6 alkyl, and the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, and C1-6 haloalkoxy.

    [0010] In this specification, a substituted group refers to a group in which at least one hydrogen atom is replaced with at least one non-hydrogen atom group, provided that the group satisfies the valence electron requirements and forms a chemically stable compound from the substitution. Unless explicitly described as unsubstituted in this specification, it should be understood that all substituents will be unsubstituted or substituted with another substituent. The substituents R1 to R3 on the biguanide derivative according to the present invention may each be re-substituted with at least one of the above-defined substituents.

    [0011] The term halogen or halo- refers to fluoro, chloro, bromo, and iodo.

    [0012] The term hydroxy refers to -OH.

    [0013] The term alkyl refers to a linear and branched saturated hydrocarbon group generally having a specified number of carbon atoms (for example, 1 to 12 carbon atoms). Examples of the alkyl group include, without limitation, methyl, ethyl, n-propyl, i-propyl, n-butyl, s-butyl, i-butyl, t-butyl, pent-1-yl, pent-2-yl, pent-3-yl, 3-methylbut-1-yl, 3-methylbut-2-yl, 2-methylbut-2-yl, 2,2,2-trimethyleth-1-yl, n-hexyl, n-heptyl, and n-octyl, etc. The alkyl may be attached to a parent group or a substrate at any ring atom, unless the attachment would violate the valence electron requirements. Likewise, the alkyl group may include at least one non-hydrogen substituent unless the substitution would violate the valence electron requirements. For example, the term haloalkyl refers to an alkyl group in which at least one hydrogen atom of the alkyl group is substituted with halogen. For example, when at least one hydrogen atom of methyl group is substituted with halogen, group such as -CH2(halo) -CH(halo)2 or C(halo)3 is formed. Examples of the term haloalkyl group include, without limitation, trifluoromethyl, trichloromethyl, tribromomethyl, and triiodomethyl.

    [0014] The term alkoxy refers to alkyl-O-, provided that the alkyl is the same as defined above. Examples of the alkoxy group include, without limitation, methoxy, ethoxy, n-propoxy, i-propoxy, n-butoxy, s-butoxy, t-butoxy, n-pentoxy, s-pentoxy, etc. The alkoxy may be attached to a parent group or a substrate at any ring atom, unless the attachment would violate the valence electron requirements. Likewise, the alkoxy group may include at least one non-hydrogen substituent unless the attachment would violate the valence electron requirements. For example, the term haloalkoxy refers to an alkoxy group in which at least one hydrogen atom of the alkoxy group is substituted with halogen. For example, when at least one hydrogen atom of methoxy group is substituted with halogen, group such as -O-CH2(halo), -O-CH(halo)2 or -O-C(halo)3 is formed. Examples of the term haloalkoxy group include, without limitation, trifluo-romethoxy, trichloromethoxy, tribromomethoxy, and triiodomethoxy, etc.

    [0015] The term cycloalkene refers to a saturated monocyclic and polycyclic hydrocarbon ring generally having the specified number of carbon atoms (for example, C3-8 cycloalkene refers to cycloalkene group having 3, 4, 5, 6, 7 or 8 carbon atoms as ring members). The cycloalkene may be attached to a parent or substrate at any ring atom, unless the attachment would violate the valence electron requirements. Likewise, the cycloalkene group may include at least one non-hydrogen substituent unless the substitution would violate the valence electron requirements.

    [0016] The term heterocycloalkene refers to an unsaturated non-aromatic monocyclic and polycyclic hydrocarbon ring in which at least one of ring members in the cycloalkene is composed of elements rather than carbon, including heteroatoms, for example, nitrogen, oxygen or sulfur. The heterocycloalkene may be attached to a parent or substrate at any ring atom, unless the attachment would violate the valence electron requirements. Likewise, the heterocycloalkene group may include at least one non-hydrogen substituent unless the attachment would violate the valence electron requirements. Examples of the heterocycloalkene group include, without limitation, dihydroazetine, dihydropyrrole, dihydropyridine, tetrahydropyridine, dihydroazepine, tetrahydroazepine, etc.

    [0017] The term cycloalkyl refers to a saturated monocyclic and dicyclic hydrocarbon ring generally having the specified number of carbon atoms included in a ring (that is, C3-8 cycloalkyl refers to cycloalkyl group having 3, 4, 5, 6, 7 or 8 carbon atoms as ring members). The cycloalkyl may be attached to a parent or substrate at any ring atom, unless the attachment would violate the valence electron requirements. Likewise, the cycloalkyl group may include at least one non-hydrogen substituent unless the substitution would violate the valence electron requirements.

    [0018] The term heterocycloalkyl refers to a monocyclic and dicyclic hydrocarbon ring in which at least one of ring system atoms in the cycloalkyl is composed of elements rather than carbon, including heteroatoms, that is, nitrogen, oxygen or sulfur. The heterocycloalkyl may be attached to a parent or substrate at any ring atom, unless the attachment would violate the valence electron requirements. Likewise, the heterocycloalkyl group may include at least one non-hydrogen substituent unless the substitution would violate valence electron requirements. Examples of the heterocycloalkyl group include, without limitation, aziridine, azetidine, imidazolyl, pyrrolyl, pyrrolidinyl, piperidyl, morpholinyl, piperazinyl, azepanyl, indolyl, indolinyl, etc.

    [0019] According to one exemplary embodiment, R1 and R2 may be taken together with nitrogen to which they are attached to form C4-5 heterocycloalkene having 5 to 6 ring atoms or C4-5 heterocycloalkyl having 5 to 6 ring atoms or piperidinyl, which is substituted with C1-4 alkyl in at least one position thereof; and
    R3 may be hydrogen; C1-6 alkyl; phenyl; or C1-4 alkyl substituted with a phenyl,
    wherein the C4-5 heterocycloalkene is unsubstituted or substituted with C1-6 alkyl,
    the C4-5 heterocycloalkyl is substituted with 1 to 4 C1-6 alkyl groups, and
    the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, and C1-6 haloalkoxy.

    [0020] According to another exemplary embodiment, R1 and R2 may be taken together with nitrogen to which they are attached to form C4-5 heterocycloalkene having 5 to 6 ring atoms or piperidinyl, which is substituted with one or two C1-2 alkyl groups.

    [0021] R3 may be hydrogen; C1-6 alkyl; phenyl; or C1-2 alkyl substituted with phenyl,
    wherein the C4-5 heterocycloalkene is unsubstituted or substituted with C1-2 alkyl and
    the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, C1-2 alkoxy, C1-2 haloalkyl, and C1-2 haloalkoxy.

    [0022] According to still another exemplary embodiment, R1 and R2 may be taken together with nitrogen to which they are attached to form C3-6 heterocycloalkene selected from the group consisting of dihydroazetinyl; dihydropyrrolinyl; dihydropyridinyl; and tetrahydropyridinyl, or R1 and R2 may be taken together with nitrogen to which they are attached to form piperidinyl, which is substituted with C1-4 alkyl in at least one position thereof;
    and
    R3 may be hydrogen; C1-6 alkyl; phenyl; or C1-4 alkyl substituted with phenyl,
    wherein the C3-6 heterocycloalkene is unsubstituted or substituted with C1-6 alkyl,
    , and
    the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, and C1-6 haloalkoxy.

    [0023] According to still another exemplary embodiment, R1 and R2 may be taken together with nitrogen to which they are attached to form dihydropyrrolinyl or tetrahydropyridinyl which is unsubstituted or substituted with C1-4 alkyl, or R1 and R2 may be taken together with nitrogen to which they are attached to form piperidinyl which is substituted with C1-4 alkyl in at least one position thereof, and
    R3 may be hydrogen; C1-6 alkyl; phenyl; or C1-4 alkyl substituted with phenyl,
    wherein the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, C1-4 alkoxy, C1-4 haloalkyl, and C1-4 haloalkoxy.

    [0024] According to still another exemplary embodiment, R1 and R2 may be taken together with nitrogen to which they are attached to form dihydropyrrolinyl or tetrahydropyridinyl which is unsubstituted or substituted with C1-2 alkyl, or R1 and R2 may be taken together with nitrogen to which they are attached to form piperidinyl or pyrrolidinyl substituted with one or two C1-2 alkyl groups, and
    R3 may be hydrogen; C3-6 alkyl; phenyl; or C1-2 alkyl substituted with phenyl,
    wherein the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, C1-2 alkoxy, C1-2 haloalkyl, and C1-2 haloalkoxy.

    [0025] According to yet another exemplary embodiment, R1 and R2 may be taken together with nitrogen to which they are attached to form dihydropyrrolinyl or tetrahydropyridinyl which is unsubstituted or substituted with methyl, or R1 and R2 may be taken together with nitrogen to which they are attached to form piperidine, and
    R3 may be hydrogen; butyl; propyl; hexyl; phenyl; or methyl substituted with phenyl,
    wherein the piperidine is substituted with one or two methyl groups in at least one of positions 2, 3, 5 and 6, and
    the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, methoxy, trihalomethyl, and tri-halomethoxy.

    [0026] According to one exemplary embodiment, the compound of Formula 1 may include

    N1-1,2-dihydropyrrole-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-trifluoromethyl)phenyl biguanide;

    N1 -1,2-dihydropyrrole-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-fluoro)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-chloro)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-bromo)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-chloro,4-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-chloro,4-trifluoromethoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-trifluoromethyl)benzyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-trifluoromethyl)benzyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-trifluoromethoxy)benzyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-chloro)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide;

    N 1-(3-methyl)-1,2-dihydropyrrole-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-fluoro)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-bromo)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-methoxy)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(3,4-dimethoxy)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethoxy)benzyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethyl)benzyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-chloro,3-trifluoromethyl)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-chloro, 3-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-fluoro,4-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-chloro)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-bromo)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-fluoro)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3,5-dimethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-NS-(4-methoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-methoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-methoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-methoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3,5-dimethoxy)phenyl biguanide;

    N1 -1,2-dihydropyrrole-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1 -1,2-dihydropyrrole-N5-(3-fluoro, 4-trifluoromethyl)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-methyl)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-methyl)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-methyl)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-methyl)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-hexyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethoxy)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethoxy)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethyl)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethyl)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-chloro, 3-trifluoromethyl)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-butyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-propyl biguanide; N1-1,2,3,6-tetrahydropyridine biguanide; N1-(3-methyl)piperidine-N5-(3-trifluoromethyl)benzyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-chloro)benzyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-fluoro)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-bromo)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(3-fluoro, 4-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(3-fluoro, 4-trifluoromethoxy)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(3-fluoro, 4-trifluoromethoxy)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(4-chloro)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-trifluoromethoxy)benzyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-trifluoromethyl)benzyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-chloro)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(3-fluoro, 4-trifluoromethoxy)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-chloro)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-bromo)phenyl biguanide; or

    N1-(2,6-dimethyl)piperidine-N5-(4-fluoro)phenyl biguanide.



    [0027] Meanwhile, a pharmaceutically acceptable salt of the compound of Formula 1 according to the present invention may be an acid addition salt formed using an organic acid or an inorganic acid. For example, the organic acid may include formic acid, acetic acid, propionic acid, lactic acid, butyric acid, isobutyric acid, trifluoroacetic acid, malic acid, maleic acid, malonic acid, fumaric acid, succinic acid, succinic acid monoamide, glutamic acid, tartaric acid, oxalic acid, citric acid, glycolic acid, glucuronic acid, ascorbic acid, benzoic acid, phthalic acid, salicylic acid, anthranilic acid, dichloroacetic acid, aminooxy acetic acid, benzensulfonic acid, 4-toluenesulfonic acid and methanesulfonic acid; and the inorganic acid may include, for example, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, carbonic acid and boric acid. For example, the above-described acid addition salt may be prepared by a typical method of preparing a salt, including a) directly mixing the compound of Formula 1 and an acid, b) dissolving one of the compound and an acid in a solvent or a hydrated solvent and mixing the resulting solution, or c) mixing the compound of Formula 1 and the acid in the presence of a solvent or a hydrated solvent.

    [0028] According to one exemplary embodiment, the pharmaceutically acceptable salt of the compound represented by Formula 1 may be a salt of an acid selected from the group consisting of formic acid, acetic acid, propionic acid, lactic acid, butyric acid, isobutyric acid, trifluoroacetic acid, malic acid, maleic acid, malonic acid, fumaric acid, succinic acid, succinic acid monoamide, glutamic acid, tartaric acid, oxalic acid, citric acid, glycolic acid, glucuronic acid, ascorbic acid, benzoic acid, phthalic acid, salicylic acid, anthranilic acid, benzensulfonic acid, p-toluenesulfonic acid, methanesulfonic acid, dichloroacetic acid, aminooxy acetic acid, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, carbonic acid, and boric acid.

    [0029] The compound of Formula 1 according to the present invention may be prepared by several methods.

    [0030] According to one exemplary embodiment, there is provided a method of preparing a compound represented by the following Formula 1, which includes reacting a compound of the following Formula 2 with a dicyanamide in an organic solvent to obtain a compound of the following Formula 3; and reacting the compound of the following Formula 3 with a compound of the following Formula 4 in an organic solvent to obtain the compound of the following Formula 1:











    [0031] In Formulas 1 to 4, R1, R2 and R3 are the same as defined in Formula 1.

    [0032] For example, the preparation method may be illustrated in the following Scheme 1, and will be described by operations, as follows.



    [0033] In the method of preparing the compound of Formula 1, the cyanoguanidine compound of Formula 3 used as an intermediate may be obtained by reacting the cyclic amine of Formula 2 with a dicyanamide such as sodium or potassium dicyanamide in an organic solvent in the presence of an acid. Then, the compound of Formula 1 may be obtained by refluxing the obtained cyanoguanidine compound of Formula 3 with the compound of Formula 4 in an organic solvent.

    [0034] An amount of the dicyanamide used for preparation of the cyanoguanidine compound of Formula 3 is equivalent to approximately 1 to 3 moles with respect to the compound of Formula 2, and an amount of the acid used is equivalent to approximately 1 to 2 moles with respect to the compound of Formula 2. Upon preparation of the compound of Formula 2, methanol, ethanol, propanol, butanol, pentanol, acetonitrile, benzene, toluene, 1,4-dioxane, N,N-dimethylamide, and the like may be, for example, used as the organic solvent, and hydrochloric acid, sulfuric acid, nitric acid, hydrobromic acid, 4-toluenesulfonic acid, and the like may be, used as the acid. The reaction temperature of the compound of Formula 2 and the dicyanamide may be in a range of 60 to 140°C, and the reaction time may be in a range of 3 to 24 hours.

    [0035] After the cyanoguanidine compound of Formula 3 obtained above is dissolved in an organic solvent, the compound of Formula 4 and an acid are added, and then stirred under reflux. In this case, an amount of the compound of Formula 4 is equivalent to approximately 1 to 2 moles with respect to the compound of Formula 3, and an amount of the acid is equivalent to approximately 1 to 2 moles with respect to the compound of Formula 3. For example, the organic solvent used in reaction of the compound of Formula 3 and the compound of Formula 4 may include methanol, ethanol, propanol, butanol, pentanol, acetonitrile, benzene, toluene, 1,4-dioxane, N,N-dimethylamide, and the like, and the acid may, for example, include hydrochloric acid, sulfuric acid, nitric acid, hydrobromic acid, 4-toluenesulfonic acid, and the like. In this case, the reaction temperature may be in a range of a reflux temperature of the solvent used (i.e., 120 to 140°C for butanol), and the reaction time may be in a range of 6 to 24 hours. When the reaction is completed, the resulting reaction solution is filtered. Thereafter, a pH of the filtered reaction solution may be controlled to approximately 4 to 5 using an acid such as, hydrochloric acid. Then, the resulting reaction solution may be concentrated and purified to yield the compound of Formula 1 or a pharmaceutically acceptable salt thereof according to the present invention.

    [0036] The compound of Formula 1 or the pharmaceutically acceptable salt thereof produced in this way may be useful in performing anticancer treatment including inhibition of cancer metastasis and cancer recurrence by AMPK activation, even when administered in a small dose compared with conventional drugs, as will be confirmed in the following Examples.

    [0037] Therefore, the present invention provides a medicine including the compound of Formula 1 or the pharmaceutically acceptable salt thereof as an active ingredient.

    [0038] Another aspect of the present invention provides a pharmaceutical composition for preventing or treating a cancer, which includes the compound of Formula 1 or the pharmaceutically acceptable salt thereof as an active ingredient, the use of the compound of Formula 1 or the pharmaceutically acceptable salt thereof to prepare a medicine for preventing or treating the disease, and a method of preventing or treating the disease including administering a therapeutically effective amount of the compound of Formula 1 or the pharmaceutically acceptable salt thereof to a subject.

    [0039] The pharmaceutical composition of the present invention includes at least one pharmaceutically acceptable carrier in addition to the active ingredient. As used in this specification, the term pharmaceutically acceptable carrier refers to a known pharmaceutically acceptable excipient, which is useful to formulate a pharmaceutically active compound for administration, and is substantially non-toxic and non-sensitive under the conditions used. An exact ratio of the excipient is determined by standard pharmaceutical practice, as well as solubility, chemical characteristics and selected route for administration of the active compound.

    [0040] The pharmaceutical composition of the present invention may be formulated in a form suitable for a desired administration method using a suitable and physiologically available adjuvant such as an excipient, a disintegrating agent, a sweetening agent, a binder, a coating agent, a swelling agent, a lubricating agent, a glossing agent, a flavoring agent, or the like.

    [0041] The pharmaceutical composition may be formulated as a tablet, a capsule, a pill, a granule, a powder, an injection or a liquid, but the present invention is not limited thereto.

    [0042] The formulation and the pharmaceutically acceptable carrier of the pharmaceutical composition may be properly selected according to the techniques known in the related art, and, for example, may be selected with reference to the following documents: (Urquhart et al., Lancet, 16:367, 1980); (Lieberman et al., PHARMACEUTICAL DOSAGE FORMS-DISPERSE SYSTEMS, 2nd ed., vol. 3, 1998); (Ansel et al., PHARMACEUTICAL DOSAGE FORMS & DRUG DELIVERY SYSTEMS, 7th ed., 2000); (Martindale, THE EXTRA PHARMACOPEIA, 31st ed.); (Remington's PHARMACEUTICAL SCIENCES, 16th-20th editions); (THE PHARMACOLOGICAL BASIS OF THERAPEUTICS, Goodman and Gilman, eds., 9th ed., 1996); and (Wilson and Gisvolds' TEXTBOOK OF ORGANIC MEDICINAL AND PHARMACEUTICAL CHEMISTRY, Delgado and Remers, eds., 10th ed., 1998). Also, principals of formulating a pharmaceutical composition may be described, for example, with reference to the following documents: (Platt, Clin Lab Med, 7:289-99, 1987); (Aulton, PHARMACEUTICS: THE SCIENCE OF DOSAGE FORM DESIGN, Churchill Livingstone, NY, 1988); (EXTEMPORANEOUS ORAL LIQUID DOSAGE PREPARATIONS, CSHP, 1998); and (Drug Dosage, J Kans Med Soc, 70(1):30-32, 1969).

    [0043] According to one exemplary embodiment, the pharmaceutical composition may be used together with a second drug.

    [0044] According to the present invention, the term second drug refers to another pharmaceutically active ingredient in addition to the biguanide derivative according to the present invention. The compound of Formula 1 or the pharmaceutically acceptable salt thereof according to the present invention may be used to treat a variety of diseases, as described above. As a result, the compound of Formula 1 or the pharmaceutically acceptable salt thereof according to the present invention may be used together with a second drug for effectively treating respective diseases. For example, the second drug may be an anticancer agent, an anti-hyperglycemic agent, an anti-obesity agent, etc., which includes an active ingredient different from the compound of Formula 1 or the pharmaceutically acceptable salt thereof.

    [0045] When the compound of Formula 1 or the pharmaceutically acceptable salt thereof according to the present invention and the second drug are able to be administered in the same manner, the compound of Formula 1 or the pharmaceutically acceptable salt thereof may be formulated together with the second drug to be provided in the form of a composite preparation.

    [0046] Meanwhile, according to the present invention, the term subject refers to a warm-blooded animal such as a mammal with a specific condition, disorder or disease. For example, the subject may be a human, an orangutan, a chimpanzee, a mouse, a rat, a dog, a cow, a chicken, a pig, a goat, a sheep, etc., but the present invention is not limited thereto.

    [0047] Also, the term treating includes relieving a symptom, temporarily or permanently eliminating causes of the symptom, and preventing or hindering occurrence of the symptom or progression of the above-described condition, disorder or disease, but the present invention is not limited thereto.

    [0048] An effective amount of the active ingredient of the pharmaceutical composition according to the present invention refers to an amount required to treat a disease. Therefore, the effective amount of the active ingredient may be adjusted according to various factors such as kinds and severity of a disease, kinds and contents of an active ingredient and other ingredients included in the composition, kinds of a formulation, age, body weight, general medical conditions, sex and diet of a patient, duration and route of administration, a release rate of the composition, treatment duration, and the number of drugs used together. In the case of adults, for example, the compound of Formula 1 may be administered in a total dose of 50 to 3,000 mg/kg when administered once to several times a day.

    Advantageous Effects of Invention



    [0049] The N1-cyclic amine-N5-substituted biguanide derivative of Formula 1 according to the present invention can be highly useful in treating a cancer since the N1-cyclic amine-N5-substituted biguanide derivative of Formula 1 has an effect of inhibiting cancer cell proliferation, cancer metastasis and recurrence even when administered in a small dose compared with conventional drugs.

    Mode for the Invention



    [0050] The advantages and features of the present invention and the method of revealing them will be explicit from the following examples described in detail. However, it is to be distinctly understood that the present invention is not limited thereto but may be otherwise variously embodied and practiced. It is obvious that the following examples are to complete the disclosure of the invention and to indicate the scope of the present invention to a skilled artisan completely, and the present invention will be defined only by the scope of the claims.

    [EXAMPLES]


    [Example 1: Synthesis of N1-2,5-dihydropyrrole cyanoguanidine



    [0051] 



    [0052] 2,5-Dihydropyrrole hydrochloride (0.75 g, 7.104 mmol) and sodium dicyanamide (0.63 g, 7.104 mmol) were dissolved in a butanol (20 mL) solution, and then stirred for 3 hours under reflux. After completion of the reaction was confirmed, sodium chloride formed by filtering the reaction mixture was removed, and the filtrate was then concentrated at a reduced pressure. The concentrate was dissolved in methanol (2 mL), and ethyl acetate (5 mL) was then added thereto, and stirred at room temperature for an hour. The formed solid was filtered and the filtrate was washed with ethyl acetate (2x20 mL). The filtrate was dried at a reduced pressure to obtain a white solid target compound (0.90 g, 93%).
    1H NMR (600 MHz, CD3OD) δ 5.89 (m, 2H), 4.16 (m, 4H); LC-MS m/z 137.2 [M+1]+

    Example 2: Synthesis of N1-1,2,3,6-tetrahydropyridine cyanoguanidine



    [0053] 



    [0054] A white solid target compound (2.23 g, 59.2%) was prepared in the same manner as in Example 1, except that 1,2,3,6-tetrahydropyridine was used instead of the 2,5-dihydropyrrole hydrochloride used in Example 1.
    1H NMR (600 MHz, CD3OD) δ 5.90 (d, 1H), 5.69 (d, 1H), 3.93 (t, 2H), 3.59(t, 2H), 2.18 (m, 2H); LC-MS m/z 151.2 [M+1]+

    Example 3: Synthesis of N1-3-methyl-2,5-dihydropyrrole cyanoguanidine



    [0055] 



    [0056] A white solid target compound (3.12 g, 83%) was prepared in the same manner as in Example 1, except that 3-methyl-2,5-dihydropyrrole was used instead of the 2,5-dihydropyrrole hydrochloride used in Example 1.
    1H NMR (600 MHz, CD3OD) δ 5.45 (br s, 1H), 4.80 (m, 2H), 4.10 (m, 2H) 7.16 (s, 3H); LC-MS m/z 151.2 [M+1]+

    Example 4: Preparation of N1-1,2-dihydropyrrole-N5-(4-trifluoromethoxy)phenyl biguanide hydrochloride



    [0057] 



    [0058] (4-Trifluoromethoxy)phenylamine (390 mg, 2.20 mmol) was dissolved in a butanol (10 mL) solution at room temperature, and concentrated hydrochloric acid (0.18 mL, 2.20 mmol) was added to the resulting solution, and then stirred for 30 minutes. The N1-2,5-dihydropyrrole cyanoguanidine (300 mg, 2.20 mmol) obtained in Example 1 was added to the reaction mixture, and then stirred for an hour under reflux. The reaction mixture was stirred at room temperature for an hour, and the formed solid was filtered. Then, the filtrate was dried at a reduced pressure to obtain a white solid target compound (265 mg, 34%).
    1H NMR (600 MHz, CD3OD) δ 3.94 (m, 2H), 3.71 (m, 2H), 3.47 (m, 1H), 3.34 (s, 3H), 2.90 (m, 1H), 2.57 (m, 1H), 1.71 (m, 10H), 1.18 (m, 1H), 0.89 (s, 3H); LC-MS m/ z 282.2 [M+1]+; mp 172-174°C

    [0059] Target compounds of the following Examples 5 to 61 were prepared in the same manner as in Example 4, except that the cyanoguanidine and amine compounds synthesized in Examples 2 and 3, which corresponded to the target compounds, were used respectively instead of the N1-2,5-dihydropyrrole cyanoguanidine synthesized in Example 1 and the (4-trifluoromethoxy)phenylamine used in Example 4.

    Example 5: Preparation of N1-1,2-dihydropyrrole-N5-(4-trifluoromethyl)phenyl biguanide hydrochloride



    [0060] 



    [0061] 1H NMR (600 MHz, CD3OD) δ 7.58 (m, 4H), 5.91 (m, 2H), 4.27 (m, 4H); LC-MS m/z 298.2 [M+1]+; mp 254-256°C

    Example 6: Preparation of N1-1,2-dihydropyrrole-N5-(3-trifluoromethyl)phenyl biguanide hydrochloride



    [0062] 



    [0063] 1H NMR (600 MHz, CD3OD) δ 7.79 (m, 1H), 7.56 (m, 1H), 7.46 (m, 1H), 7.34 (m, 1H), 5.91 (s, 2H), 4.20 (m, 4H); LC-MS m/z 298.2 [M+1]+; mp 276-278°C

    Example 7: Preparation of N1-1,2-dihydropyrrole-N5-(4-fluoro)phenyl biguanide hydrochloride



    [0064] 



    [0065] 1H NMR (600 MHz, CD3OD) δ 7.33 (m, 2H), 7.04 (m, 2H), 5.90 (m, 2H), 4.17 (m, 4H); LC-MS m/z 248.2 [M+1]+; mp 263-265°C

    Example 8: N-1,2-dihydropyrrole-N5-(4-chloro)phenyl biguanide hydrochloride



    [0066] 



    [0067] 1H NMR (600 MHz, CD3OD) δ 7.29 (m, 4H), 5.90 (m, 2H), 4.20 (m, 4H); LC-MS m/z 264.2 [M+1]+; mp 264-266°C

    Example 9: N1-1,2-dihydropyrrole-N5-(4-bromo)phenyl biguanide hydrochloride



    [0068] 



    [0069] 1H NMR (600 MHz, CD3OD) δ 7.42 (m, 2H), 7.30 (m, 2H), 5.90 (m, 2H), 4.24 (m, 4H); LC-MS m/z 309.0 [M+1]+; mp 263-265°C

    Example 10: N1-1,2-dihydropyrrole-N5-(3-chloro,4-trifluoromethoxy)phenyl biguanide hydrochloride



    [0070] 



    [0071] 1H NMR (600 MHz, CD3OD) δ 7.79 (m, 1H), 7.42 (m, 2H), 5.99 (m, 2H), 4.30 (m, 4H); LC-MS m/z 348.2 [M+1]+; mp 270-272°C

    Example 11: N1-1,2,3,6-tetrahydropyridine-N5-(3-chloro,4-trifluoromethoxy) phenyl biguanide hydrochloride



    [0072] 



    [0073] 1H NMR (600 MHz, CD3OD) δ 7.70 (m, 1H), 7.33 (m, 2H), 5.94 (m, 1H), 5.73 (m, 1H), 3.99 (m, 2H), 3.63 (m, 2H), 2.23 (m, 2H); LC-MS m/z 362.2 [M+1]+; mp 250-252°C

    Example 12: N1-1,2-dihydropyrrole-N5-(3-trifluoromethyl)benzyl biguanide hydrochloride



    [0074] 



    [0075] 1H NMR (600 MHz, CD3OD) δ 7.70 (m, 4H), 5.89 (m, 2H), 4.52 (m, 2H), 4.23 (m, 4H); LC-MS m/z 312.2 [M+1]+; mp 156-158°C

    Example 13: N1-1,2-dihydropyrrole-N5-(4-trifluoromethyl)benzyl biguanide hydrochloride



    [0076] 



    [0077] 1H NMR (600 MHz, CD3OD) δ 7.45 (m, 2H), 7.62 (m, 2H), 5.86 (m, 2H), 4.19 (m, 2H), 4.13 (m, 4H); LC-MS m/z 312.2 [M+1]+; mp 268-270°C

    Example 14: N1-1,2-dihydropyrrole-N5-(3-trifluoromethoxy)benzyl biguanide hydrochloride



    [0078] 



    [0079] 1H NMR (600 MHz, CD3OD) δ 7.33 (m, 4H), 5.88 (m, 2H), 4.47 (s, 2H), 4.18 (m, 4H); LC-MS m/z 328.2 [M+1]+; mp 218-220°C

    Example 15: N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethoxy)phenyl biguanide hydrochloride



    [0080] 



    [0081] 1H NMR (600 MHz, CD3OD)δ 7.41 (m, 2H), 7.16 (m, 2H), 5.46 (m, 1H), 4.10 (m, 3H), 1.77 (m, 3H); LC-MS m/z 328.2 [M+1]+; mp 279-281°C

    Example 16: N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethyl)phenyl biguanide hydrochloride



    [0082] 



    [0083] 1H NMR (600 MHz, CD3OD) δ 7.62 (m, 4H), 5.56 (m, 1H), 4.21 (m, 4H), 1.87 (m, 3H); LC-MS m/z 312.2 [M+1]+; mp 272-274°C

    Example 17: N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-chloro)phenyl biguanide hydrochloride



    [0084] 



    [0085] 1H NMR (600 MHz, CD3OD) δ 7.36 (m, 2H), 7.29 (m, 2H), 5.50 (m, 1H), 4.13 (m, 4H), 1.80 (m, 3H); LC-MS m/z 278.2 [M+1]+; mp 264-268°C

    Example 18: N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0086] 



    [0087] 1H NMR (600 MHz, CD3OD) δ 7.92 (m, 1H), 7.58 (m, 1H), 7.50 (m, 1H), 5.51 (s, 1H), 4.17 (m, 4H), 1.82 (m, 3H); LC-MS m/z 346.2 [M+1]+; mp 274-276°C

    Example 19: N1-(3-methyl)-1,2-dihydropyrrole-N5-(3-trifluoromethyl)phenyl biguanide hydrochloride



    [0088] 



    [0089] 1H NMR (600 MHz, CD3OD) δ 7.80 (m, 1H), 7.57 (m, 1H), 7.42 (m, 1H), 7.34 (m, 1H), 5.51 (m, 1H), 4.15 (m, 4H), 1.81 (m, 3H); LC-MS m/z 312.2 [M+1]+; mp 282-284°C

    Example 20: N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-fluoro)phenyl biguanide hydrochloride



    [0090] 



    [0091] 1H NMR (600 MHz, CD3OD) δ 7.34 (m, 2H), 7.04 (m, 2H), 5.50 (m, 1H), 4.12 (m, 4H), 1.80 (m, 3H); LC-MS m/z 262.1 [M+1]+; mp 270-272°C

    Example 21: N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-bromo)phenyl biguanide hydrochloride



    [0092] 



    [0093] 1H NMR (600 MHz, CD3OD) δ 7.45 (m, 2H), 7.33 (m, 2H), 5.52 (m, 1H), 4.16 (m, 4H), 1.83 (m, 3H); LC-MS m/z 323.0 [M+1]+; mp 272-274°C

    Example 22: N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-methoxy)phenyl biguanide hydrochloride



    [0094] 



    [0095] 1H NMR (600 MHz, CD3OD) δ 7.24 (m, 2H), 6.87 (m, 2H), 5.48 (s, 1H), 3.32 (s, 3H), 1.78 (m, 3H); LC-MS m/z 274.2 [M+1]+; mp 263-265°C

    Example 23: N1-(3-methyl)-1,2-dihydropyrrole-N5-(3,4-dimethoxy)phenyl bisuanide hydrochloride



    [0096] 



    [0097] 1H NMR (600 MHz, CD3OD) δ 6.58 (m, 2H), 6.26 (m, 1H), 5.52 (m, 1H), 4.18 (m, 4H), 3.30 (s, 3H), 1.83 (m, 3H); LC-MS m/z 304.2 [M+1]+; mp 261-263°C

    Example 24: N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethoxy)benzyl biguanide hydrochloride



    [0098] 



    [0099] 1H NMR (600 MHz, CD3OD) δ 7.59 (m, 1H), 7.43 (m, 2H), 7.35 (m, 1H), 7.25 (m, 1H), 5.46 (m, 1H), 4.43 (m, 2H), 4.15 (m, 2H), 1.81 (m, 3H); LC-MS m/z 342.2 [M+1] +; mp 184-186°C

    Example 25: N1-(3-methyl)-1,2-dihydropyrrole-N5-(3-trifluoromethoxy)phenyl biguanide hydrochloride



    [0100] 



    [0101] 1H NMR (600 MHz, CD3OD) δ 7.59 (m, 1H), 7.46 (m, 2H), 7.23 (m, 1H), 5.88 (s, 1H), 4.45 (m, 2H), 4.16 (m, 2H), 1.83 (m, 3H); LC-MS m/z 328.2 [M+1]+; mp 263-265°C

    Example 26: N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethyl)benzyl biguanide hydrochloride



    [0102] 



    [0103] 1H NMR (600 MHz, CD3OD) δ 7.74 (m, 1H), 7.64 (m, 2H), 7.51 (m, 1H), 5.56 (s, 1H), 4.48 (s, 2H), 4.19 (m, 4H), 1.83 (m, 3H); LC-MS m/z 326.2 [M+1]+; mp 267-269°C

    Example 27: N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-chloro, 3-trifluoromethyl)benzyl biguanide hydrochloride



    [0104] 



    [0105] 1H NMR (600 MHz, CD3OD) δ 7.61 (m, 1H), 7.59 (m, 2H), 7.51 (m, 1H), 5.59 (s, 1H), 4.48 (s, 2H), 4.26 (m, 4H), 1.85 (s, 3H); LC-MS m/z 326.2 [M+1]+; mp 230-232°C

    Example 28: N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethoxy)phenyl biguanide hydrochloride



    [0106] 



    [0107] 1H NMR (600 MHz, CD3OD) δ 7.46 (d, 2H), 7.24 (d, 2H), 5.95 (m, 1H), 5.74 (m, 1H), 4.00 (s, 2H), 3.64 (t, 2H), 2.23 (s, 2H); LC-MS m/z 328.1 [M+1]+

    Example 29: N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethyl)phenyl biguanide hydrochloride



    [0108] 



    [0109] 1H NMR (600 MHz, CD3OD) δ 7.59 (m, 4H), 5.96 (m, 1H), 5.75 (m, 1H), 4.01 (s, 2H), 3.65 (t, 2H), 2.25 (s, 2H); LC-MS m/z 312.2 [M+1]+

    Example 30: N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethoxy)phenyl biguanide hydrochloride



    [0110] 



    [0111] 1H NMR (600 MHz, CD3OD) δ 7.82 (s, 1H), 7.57 (d, 1H), 7.49 (t, 1H), 7.38 (d, 1H), 5.97 (m, 1H), 5.74 (m, 1H), 4.01 (s, 2H), 3.65 (t, 2H), 2.24 (s, 2H)

    Example 31: N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethyl)phenyl biguanide hydrochloride



    [0112] 



    [0113] 1H NMR (600 MHz, CD3OD) δ 7.82 (s, 1H), 7.57 (d, 1H), 7.49 (t, 1H), 7.37 (d, 1H), 5.95 (m, 1H), 5.74 (m, 1H), 4.01 (s, 2H), 3.65 (t, 2H), 2.24 (s, 2H); LC-MS m/z 312.2 [M+1]+

    Example 32: N1-1,2,3,6-tetrahydropyridine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0114] 



    [0115] 1H NMR (600 MHz, CD3OD) δ 7.80 (m, 1H), 7.60 (m, 1H), 7.28 (t, 1H), 5.95 (m, 1H), 5.73 (m, 1H), 4.00 (s, 2H), 3.64 (t, 2H), 2.23 (s, 2H); LC-MS m/z 330.2 [M+1]+

    Example 33: N1-1,2,3,6-tetrahydropyridine-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0116] 



    [0117] 1H NMR (600 MHz, CD3OD) δ 7.93 (m, 1H), 7.59 (m, 1H), 7.53 (d, 1H), 5.96 (m, 1H), 5.74 (m, 1H), 4.01 (s, 2H), 3.65 (t, 2H), 2.24 (s, 2H); LC-MS m/z 346.0 [M+1]+

    Example 34: N1-1,2,3,6-tetrahydropyridine-N5-(3-fluoro, 4-trifluoromethoxy)phenyl biguanide hydrochloride



    [0118] 



    [0119] 1H NMR (600 MHz, CD3OD) δ 7.56 (m, 1H), 7.34 (t, 1H), 7.16 (m, 1H), 5.97 (m, 1H), 5.74 (m, 1H), 4.01 (s, 2H), 3.65 (t, 2H), 2.25 (s, 2H); LC-MS m/z 346.2 [M+1]+

    Example 35: N1-1,2,3,6-tetrahydropyridine-N5-(4-chloro)phenyl biguanide hydrochloride



    [0120] 



    [0121] 1H NMR (600 MHz, CD3OD) δ 7.37 (d, 2H), 7.31 (d, 2H), 5.97 (m, 1H), 5.74 (m, 1H), 3.99 (s, 2H), 3.63 (t, 2H), 2.23 (s, 2H); LC-MS m/z 278.2 [M+1]+

    Example 36: N1-1,2,3,6-tetrahydropyridine-N5-(4-bromo)phenyl biguanide hydrochloride



    [0122] 



    [0123] 1H NMR (600 MHz, CD3OD) δ 7.45 (d, 2H), 7.30 (d, 2H), 5.97 (m, 1H), 5.74 (m, 1H), 3.99 (s, 2H), 3.63 (t, 2H), 2.23 (s, 2H); LC-MS m/z 323.0 [M+1]+

    Example 37: N1-1,2,3,6-tetrahydropyridine-N5-(4-fluoro)phenyl biguanide hydrochloride



    [0124] 



    [0125] 1H NMR (600 MHz, CD3OD) δ 7.35 (d, 2H), 7.07 (d, 2H), 5.94 (m, 1H), 5.72 (m, 1H), 3.97 (s, 2H), 3.62 (t, 2H), 2.23 (s, 2H); LC-MS m/z 262.2 [M+1]+

    Example 38: N1-1,2,3,6-tetrahydropyridine-N5-(3,5-dimethoxy)phenyl biguanide hydrochloride



    [0126] 



    [0127] 1H NMR (600 MHz, CD3OD) δ 6.56 (d, 2H), 6.27 (t, 1H), 5.96 (m, 1H), 5.74 (m, 1H), 4.00 (s, 2H), 3.76 (s, 6H), 3.64 (t, 2H), 2.23 (s, 2H); LC-MS m/z 304.2 [M+1]+

    Example 39: N1-1,2,3,6-tetrahydropyridine-N5-phenyl biguanide hydrochloride



    [0128] 



    [0129] 1H NMR (600 MHz, CD3OD) δ 7.33 (m, 4H), 7.13 (m, 1H), 5.94 (m, 1H), 5.73 (m, 1H), 3.98 (s, 2H), 3.63 (t, 2H), 2.22 (s, 2H); LC-MS m/z 244.2 [M+1]+

    Example 40: N1-1,2,3,6-tetrahydropyridine-N5-(4-methoxy)phenyl biguanide hydrochloride



    [0130] 



    [0131] 1H NMR (600 MHz, CD3OD) δ 7.23 (d, 2H), 6.91 (d, 2H) 5.93 (m, 1H), 5.72 (m, 1H), 3.96 (s, 2H), 3.78 (s, 3H), 3.61 (t, 2H), 2.21 (s, 2H); LC-MS m/z 274.2 [M+1]+

    Example 41: N1-1,2,3,6-tetrahydropyridine-N5-(3-methoxy)phenyl biguanide hydrochloride



    [0132] 



    [0133] 1H NMR (600 MHz, CD3OD) δ 7.22 (t, 1H), 7.00 (t, 1H), 6.88 (m, 1H), 6.71 (m, 1H), 5.95 (m, 1H), 5.74 (m, 1H), 3.99 (s, 2H), 3.77 (s, 3H), 3.64 (t, 2H), 2.23 (s, 2H); LC-MS m/z 274.2 [M+1]+

    Example 42: N1-1,2-dihydropyrrole-N5-(4-methoxy)phenyl biguanide hydrochloride



    [0134] 



    [0135] 1H NMR (600 MHz, CD3OD) δ 7.25 (d, 2H), 6.91 (d, 2H), 5.92 (s, 2H), 4.22 (d, 4H), 3.78 (s, 3H); LC-MS m/z 260.2 [M+1]+

    Example 43: N1-1,2-dihydropyrrole-N5-(3-methoxy)phenyl biguanide hydrochloride



    [0136] 



    [0137] 1H NMR (600 MHz, CD3OD) δ 7.22 (t, 1H), 7.03 (t, 1H), 6.91 (m, 1H), 6.71 (m, 1H), 5.93 (s, 2H), 4.29 (d, 4H), 3.77 (s, 3H); LC-MS m/z 260.2 [M+1]+

    Example 44: N1-1,2-dihydropyrrole-N5-phenyl biguanide hydrochloride



    [0138] 



    [0139] 1H NMR (600 MHz, CD3OD) δ 7.37 (m, 4H), 7.12 (t, 1H), 5.93 (s, 2H), 4.27 (d, 4H); LC-MS m/z 230.2 [M+1]+

    Example 45: N1-1,2-dihydropyrrole-N5-(3,5-dimethoxy)phenyl biguanide hydrochloride



    [0140] 



    [0141] 1H NMR (600 MHz, CD3OD) δ 6.58 (d, 2H), 6.27 (t, 1H), 5.93 (m, 1H), 4.30 (d, 4H), 3.75 (s, 6H); LC-MS m/z 290.2 [M+1]+

    Example 46: N1-1,2-dihydropyrrole-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0142] 



    [0143] 1H NMR (600 MHz, CD3OD) δ 7.82 (m, 1H), 7.64 (m, 1H), 7.29 (t, 1H), 5.94 (s, 2H), 4.26 (d, 4H); LC-MS m/z 316.2 [M+1]+

    Example 47: N1-1,2-dihydropyrrole-N5-(3-fluoro, 4-trifluoromethyl)phenyl biguanide hydrochloride



    [0144] 



    [0145] 1H NMR (600 MHz, CD3OD) δ 7.21 (m, 2H), 7.15 (d, 2H), 5.94 (m, 1H), 5.73 (m, 1H), 3.97 (s, 2H), 3.62 (t, 2H), 2.30 (s, 3H), 2.22 (s, 2H); LC-MS m/z 316.2 [M+1]+

    Example 48: N1-1,2,3,6-tetrahydropyridine-N5-(4-methyl)phenyl biguanide hydrochloride



    [0146] 



    [0147] 1H NMR (600 MHz, CD3OD) δ 7.21 (m, 2H), 7.15 (d, 2H), 5.94 (m, 1H), 5.73 (m, 1H), 3.97 (s, 2H), 3.62 (t, 2H), 2.30 (s, 3H), 2.22 (s, 2H); LC-MS m/z 258.2 [M+1]+

    Example 49: N1-1,2,3,6-tetrahydropyridine-N5-(3-methyl)phenyl biguanide hydrochloride



    [0148] 



    [0149] 1H NMR (600 MHz, CD3OD) δ 7.21 (m, 3H), 6.96 (d, 2H), 5.95 (m, 1H), 5.73 (m, 1H), 3.98 (s, 2H), 3.63 (t, 2H), 2.32 (s, 3H), 2.22 (s, 2H); LC-MS m/z 258.2 [M+1]+

    Example 50: N1-1,2-dihydropyrrole-N5-(4-methyl)phenyl biguanide hydrochloride



    [0150] 



    [0151] 1H NMR (600 MHz, CD3OD) δ 7.24 (d, 2H), 7.15 (d, 2H), 5.92 (s, 2H), 4.26 (d, 4H), 2.31 (s, 3H); LC-MS m/z 244.2 [M+1]+

    Example 51: N1-1,2-dihydropyrrole-N5-(3-methyl)phenyl biguanide hydrochloride



    [0152] 



    [0153] 1H NMR (600 MHz, CD3OD) δ 7.17 (m, 3H), 6.96 (d, 1H), 5.93 (s, 2H), 4.27 (d, 4H), 2.32 (s, 3H); LC-MS m/z 244.2 [M+1]+

    Example 52: N1-1,2-dihydropyrrole-N5-(3-trifluoromethoxy)phenyl biguanide hydrochloride



    [0154] 



    [0155] 1H NMR (600 MHz, CD3OD) δ 7.83 (s, 1H), 7.61 (d, 1H), 7.49 (t, 1H), 7.38 (d, 1H), 5.94 (s, 2H), 4.28 (d, 4H)

    Example 53: N1-1,2,3,6-tetrahydropyridine-N5-hexyl biguanide hydrochloride



    [0156] 



    [0157] 1H NMR (600 MHz, CD3OD) δ 5.93 (m, 1H), 5.72 (m, 1H), 3.98 (m, 2H), 3.63 (t, 2H), 3.20 (t, 2H), 2.23 (t, 2H), 1.70-1.33 (m, 8H), 0.91 (t, 3H); LC-MS m/z 252.4 [M+1]+

    Example 54: N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethoxy)benzyl biguanide hydrochloride



    [0158] 



    [0159] 1H NMR (600 MHz, CD3OD) δ 7.43 (d, 2H), 7.25 (d, 2H), 5.91 (m, 1H), 5.68 (m, 1H), 4.43 (s, 2H), 3.90 (m, 2H), 3.52 (t, 2H), 2.16 (s, 2H); LC-MS m/z 342.2 [M+1]+

    Example 55: N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethoxy)benzyl biguanide hydrochloride



    [0160] 



    [0161] 1H NMR (600 MHz, CD3OD) δ 7.43 (t, 1H), 7.33 (d, 1H), 7.25 (s, 1H), 7.18 (d, 1H), 5.91 (m, 1H), 5.68 (m, 1H), 4.45 (s, 2H), 3.90 (t, 2H), 3.52 (t, 2H), 2.15 (s, 2H); LC-MS m/z 342.2 [M+1]+

    Example 56: N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethyl)benzyl biguanide hydrochloride



    [0162] 



    [0163] 1H NMR (600 MHz, CD3OD) δ 7.64 (d, 2H), 7.52 (d, 2H), 5.91 (m, 1H), 5.67 (m, 1H), 4.49 (s, 2H), 3.89 (t, 2H), 3.51 (t, 2H), 2.14 (s, 2H); LC-MS m/z 326.2 [M+1]+

    Example 57: N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethyl)benzyl biguanide hydrochloride



    [0164] 



    [0165] 1H NMR (600 MHz, CD3OD) δ 7.64 (s, 1H), 7.56 (m, 3H), 5.91 (m, 1H), 5.67 (m, 1H), 4.48 (s, 2H), 3.89 (t, 2H), 3.51 (t, 2H), 2.15 (s, 2H); LC-MS m/z 326.2 [M+1]+

    Example 58: N1-1,2,3,6-tetrahydropyridine-N5-(4-chloro, 3-trifluoromethlbenzl biguanide hydrochloride



    [0166] 



    [0167] 1H NMR (600 MHz, CD3OD) δ 7.74 (s, 1H), 7.56 (m, 2H), 5.91 (m, 1H), 5.68 (m, 1H), 4.45 (s, 2H), 3.89 (t, 2H), 3.51 (t, 2H), 2.15 (s, 2H); LC-MS m/z 360.2 [M+1]+

    Example 59: N1-1,2,3,6-tetrahydropyridine-N5-butyl biguanide hydrochloride



    [0168] 



    [0169] 1H NMR (600 MHz, CD3OD) δ 5.94 (m, 1H), 5.73 (m, 1H), 4.19 (t, 1H), 3.98 (m, 2H), 3.64 (t, 2H), 3.20 (t, 1H), 2.23 (t, 2H), 1.70-1.37 (m, 4H), 0.95 (m, 3H); LC-MS m/z 224.2 [M+1]+

    Example 60: N1-1,2,3,6-tetrahydropyridine-N5-propyl biguanide hydrochloride



    [0170] 



    [0171] 1H NMR (600 MHz, CD3OD) δ 5.94 (m, 1H), 5.73 (m, 1H), 3.97 (m, 2H), 3.62 (t, 2H), 3.16 (t, 2H), 2.23 (t, 2H), 1.58(m, 2H), 0.95 (m, 3H); LC-MS m/z 210.2 [M+1]+

    Example 61: N1-1,2,3,6-tetrahydropyridine biguanide hydrochloride



    [0172] 



    [0173] 1H NMR (600 MHz, CD3OD) δ 5.97 (m, 1H), 5.77 (m, 1H), 3.98 (m, 2H), 3.64 (m, 2H), 2.40 (m, 1H), 2.23 (m, 1H); LC-MS m/z 168.2 [M+1]+

    Example 62: Synthesis of N1-(3-methyl)piperidine cyanoguanidine



    [0174] 



    [0175] Concentrated hydrochloric acid (0.9 mL, 0.010 mol) was added to a butanol (10 mL) solution containing 3-methylpiperidine (1.00 g, 0.010 mol), and stirred at 0°C for 30 minutes. Sodium dicyanamide (0.99 g, 0.011 mol) was added to the mixed solution, and the resulting reaction mixture was stirred for 24 hours under reflux.

    [0176] After completion of the reaction was confirmed, sodium chloride formed by filtering the reaction mixture was removed, and the filtrate was then concentrated at a reduced pressure. Ethyl acetate (10 mL) was then added to the concentrate, and stirred at room temperature for an hour. The formed solid was filtered, and the filtrate was washed with ethyl acetate (2x20 mL). The filtrate was dried at a reduced pressure to obtain a white solid target compound (1.34 g, 80.0%).
    1H NMR (600 MHz, CD3OD) δ 3.97 (m, 2H), 2.85 (m, 1H), 2.52 (m, 1H), 1.84 -1.45 (m, 4H), 1.17 (m, 1H), 0.92 (d, 3H)

    Example 63: Synthesis of N1-(2-methl)ieridine cyanoguanidine



    [0177] 



    [0178] A white solid target compound (1.0 g, 37%) was prepared in the same manner as in Example 62, except that N1-(2-methyl)piperidine was used instead of the N1-(3-methyl)piperidine used in Example 62.
    1H NMR (600 MHz, CD3OD) δ 3.98 (m, 2H), 2.85 (m, 1H), 2.53 (m, 1H), 1.82 (m, 1H), 1.71 (m, 1H), 1.59 (m, 1H), 1.48 (m, 1H), 1.91 (s, 3H); LC-MS m/z 167.2 [M+1]+

    Example 64: Synthesis of N1-(2,6-dimethyl)piperidine cyanoguanidine



    [0179] 



    [0180] A white solid target compound (0.75 g, 47.2%) was prepared in the same manner as in Example 62, except that N1-(2,6-dimethyl)piperidine was used instead of the N1-(3-methyl)piperidine used in Example 62.
    1H NMR (600 MHz, CD3OD) δ 4.31 (s, 2H), 1.85 (m, 1H), 1.70 (m, 4H), 1.50 (m, 1H), 1.48 (d, 6H); LC-MS m/z 181.2 [M+1]+

    Example 65: N1-(3-methyl)piperidine-N5-(3-trifluoromethyl)benzyl biguanide hydrochloride



    [0181] 



    [0182] Concentrated hydrochloric acid (0.24 mL, 2.71 mmol) was added to a butanol (10 mL) solution containing (3-trifluoromethyl)benzylamine (0.28 mL, 1.99 mmol) at room temperature, and then stirred for 30 minutes. The N1-(3-methyl)piperidine cyanoguanidine (300 mg, 1.81 mmol) obtained in Example 1 was added to the reaction mixture, and then stirred for 2 hours under reflux. The reaction mixture was concentrated at a reduced pressure, and ethyl acetate (3 mL) was added to the concentrated reaction mixture. The formed solid was filtered, and the filtrate was then dried at a reduced pressure to obtain a white solid target compound (0.37 g, 54.7%).
    1H NMR (600 MHz, CD3OD) δ 7.63 (m, 1H), 7.55 (m, 3H), 4.47 (s, 2H), 3.85 (dd, 2H), 2.86 (t, 1H), 2.54 (m, 1H), 1.80 (m, 1H), 1.67 (m, 1H), 1.56 (m, 1H), 1.47 (m, 1H), 1.17 (q, 1H), 0.85 (d, 3H); LC-MS m/z 342.2 [M+1]+

    [0183] Target compounds of the following Examples 66 to 98 were prepared in the same manner as in Example 65, except that the cyanoguanidine and amine compounds synthesized in Examples 63 and 64, which corresponded to the target compounds, were used respectively instead of the N1-(3-methyl)piperidine cyanoguanidine synthesized in Example 62 and the (3-trifluoromethyl)benzylamine used in Example 65.

    Example 66: N1-(3-methyl)piperidine-N5-(4-chloro)benzyl biguanide hydrochloride



    [0184] 



    [0185] 1H NMR (600 MHz, CD3OD) δ 7.28 (m, 4H), 4.29 (m, 2H), 3.82 (m, 2H), 2.81 (m, 1H), 2.51 (m, 1H), 1.57 (m, 4H), 1.15 (m, 1H) 0.84 (m, 3H); LC-MS m/z 308.2 [M+1] +; mp 256-258°C

    Example 67: N1-(3-methyl)piperidine-N5-(4-fluoro)phenyl biguanide hydrochloride



    [0186] 



    [0187] 1H NMR (600 MHz, CD3OD) δ 7.40 (m, 4H), 4.29 (m, 2H), 3.89 (m, 2H), 2.94 (m, 1H), 2.67 (m, 1H), 1.70 (m, 4H), 1.18 (m, 1H) 0.87 (m, 3H); LC-MS m/z 278.2 [M+1] +; mp 265-267°C

    Example 68: N1-(3-methyl)piperidine-N5-(4-bromo)phenyl biguanide hydrochloride



    [0188] 



    [0189] 1H NMR (600 MHz, CD3OD) δ 7.36 (m, 2H), 7.06 (m, 2H), 3.89 (m, 2H), 2.89 (m, 1H), 2.61 (m, 1H), 1.66 (m, 4H), 1.17 (m, 1H), 0.86 (m, 3H); LC-MS m/z 339.2 [M+1] +; mp 252-254°C

    Example 69: N1-(3-methyl)piperidine-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0190] 



    [0191] 1H NMR (600 MHz, CD3OD) δ 7.93 (m, 1H), 7.51 (m, 2H), 3.92 (m, 2H), 2.99 (m, 1H), 2.69 (m, 1H), 1.86 (m, 1H), 1.73 (m, 2H), 1.56 (m, 1H), 1.20 (m, 1H), 0.91 (m, 3H); LC-MS m/z 362.2 [M+1]+; mp 230-232°C

    Example 70: N1-(3-methyl)piperidine-N5-(3-fluoro, 4-trifluoromethyl)phenyl biguanide hydrochloride



    [0192] 



    [0193] 1H NMR (600 MHz, CD3OD) δ 7.57 (m, 2H), 7.21 (m, 1H), 3.92 (m, 2H), 3.00 (m, 1H), 2.72 (m, 1H), 1.88 (m, 1H), 1.71 (m, 3H), 1.24 (m, 1H), 0.91 (m, 3H); LC-MS m/ z 346.2 [M+1]+; mp 228-230°C

    Example 71: N1-(3-methyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0194] 



    [0195] 1H NMR (600 MHz, CD3OD) δ 7.85 (m, 2H), 7.60 (m, 1H), 7.29 (t, J = 9.6Hz, 1H), 3.93 (m, 2H), 3.02 (m, 1H), 2.70 (m, 1H), 1.88 (m, 1H), 1.77 (m, 2H), 1.58 (m, 1H), 1.25 (m, 1H), 0.95 (m, 3H); LC-MS m/z 346.2 [M+1]+; mp 243-245°C

    Example 72: N1-(2-methyl)piperidine-N5-(4-trifluoromethoxy)phenyl biguanide hydrochloride



    [0196] 



    [0197] 1H NMR (600 MHz, CD3OD) δ 7.42 (m, 2H), 7.20 (m, 2H), 4.35 (m, 1H), 3.88 (m, 1H), 3.06 (m, 1H), 1.71 (m, 3H), 1.59 (m, 2H), 1.48 (m, 1H), 1.22 (s, 3H); LC-MS m/z 344.2 [M+1]+; mp 250-252°C

    Example 73: N1-(2-methyl)piperidine-N5-(3-trifluoromethoxy)phenyl biguanide hydrochloride



    [0198] 



    [0199] 1H NMR (600 MHz, CD3OD) δ 7.52 (m, 1H), 7.37 (m, 1H), 7.25 (m, 1H), 6.96 (m, 1H), 4.41 (m, 1H), 3.91 (m, 1H), 3.13 (m, 1H), 1.74 (m, 3H), 1.64 (m, 2H), 1.51 (m, 1H) 1.26 (m, 3H); LC-MS m/z 344.2 [M+1]+; mp 246-248°C

    Example 74: N1-(2-methyl)piperidine-N5-(4-trifluoromethyl)phenyl biguanide hydrochloride



    [0200] 



    [0201] 1H NMR (600 MHz, CD3OD) δ 7.56 (m, 4H), 4.37 (m, 1H), 3.91 (m, 1H), 3.12 (m, 1H), 1.73 (m, 3H), 1.61 (m, 2H), 1.52 (m, 1H) 1.24 (m, 3H); LC-MS m/z 329.2 [M+1] +; mp 252-254°C

    Example 75: N1-(3-methyl)piperidine-N5-(3-fluoro, 4-trifluoromethoxy)phenyl biguanide hydrochloride



    [0202] 



    [0203] 1H NMR (600 MHz, CD3OD) δ 7.50 (m, 1H), 7.23 (m, 1H), 7.08 (m, 1H), 2.85 (m, 2H), 2.63 (m, 1H), 1.79 (m, 1H), 1.67 (m, 3H), 1.58 (m, 1H), 1.17 (m, 1H), 0.86 (m, 3H); LC-MS m/z 362.2 [M+1]+; mp 243-245°C

    Example 76: N1-(2-methyl)piperidine-N5-(3-fluoro, 4-trifluoromethoxy)phenyl biguanide hydrochloride



    [0204] 



    [0205] 1H NMR (600 MHz, CD3OD) δ 7.58 (m, 1H), 7.36 (m, 1H), 7.16 (m, 1H), 4.41 (m, 1H), 3.92 (m, 1H), 3.16 (m, 1H), 1.70 (m, 6H) 1.29 (m, 3H); LC-MS m/z 362.0 [M+1] +; mp 241-243°C

    Example 77: N1-(2-methyl)piperidine-N5-(4-chloro)phenyl biguanide hydrochloride



    [0206] 



    [0207] 1H NMR (600 MHz, CD3OD) δ 7.30 (m, 4H), 4.36 (m, 1H), 3.89 (m, 1H), 3.10 (m, 1H), 1.61 (m, 5H), 1.49 (m, 1H), 1.25 (m, 3H); LC-MS m/z 294.0 [M+1]+; mp 251-253°C

    Example 78: N1-(2-methyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0208] 



    [0209] 1H NMR (600 MHz, CD3OD) δ 7.84 (m, 1H), 7.56 (m, 1H), 7.28 (m, 1H), 4.39 (m, 1H), 3.90 (m, 1H), 3.13 (m, 1H), 1.73 (m, 5H), 1.51 (m, 1H), 1.25 (m, 3H); LC-MS m/ z 346.0 [M+1]+; mp 251-253°C

    Example 79: N1-(2-methyl)piperidine-N5-(3-trifluoromethyl)phenyl biguanide hydrochloride



    [0210] 



    [0211] 1H NMR (600 MHz, CD3OD) δ 7.83 (m, 1H), 7.48 (m, 2H), 7.32 (m, 1H), 4.39 (m, 1H), 3.92 (m, 1H), 3.13 (m, 1H), 1.73 (m, 6H), 1.20 (m, 3H); LC-MS m/z 328.2 [M+1] +; mp 249-251°C

    Example 80: N1-(2-methyl)piperidine-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0212] 



    [0213] 1H NMR (600 MHz, CD3OD) δ 7.83 (m, 1H), 7.95 (m, 1H), 7.51 (m, 2H), 4.38 (m, 1H), 3.91 (m, 1H), 3.12 (m, 1H), 1.60 (m, 6H), 1.20 (m, 3H); LC-MS m/z 362.3 [M+1] +; mp 316-318°C

    Example 81: N1-(3-methyl)piperidine-N5-(4-trifluoromethyl)phenyl biguanide hydrochloride



    [0214] 



    [0215] 1H NMR (600 MHz, CD3OD) δ 7.61 (s, 4H), 3.98 (d, 2H), 3.04 (t, 1H), 2.74 (t, 1H), 1.91 (m, 1H), 1.81-1.73 (m, 2H), 1.63 (m, 1H), 1.27 (q, 1H), 0.98 (d, 3H); LC-MS m/z 328.2 [M+1]+

    Example 82: N1-(3-methyl)piperidine-N5-(4-trifluoromethoxy)phenyl biguanide hydrochloride



    [0216] 



    [0217] 1H NMR (600 MHz, CD3OD) δ 7.46 (m, 2H), 7.22 (m, 2H), 3.94 (d, 2H), 2.97 (t, 1H), 2.67 (t, 1H), 1.86 (m, 1H), 1.76-1.66 (m, 2H), 1.55 (m, 1H), 1.23 (q, 1H), 0.93 (d, 3H); LC-MS m/z 344.2 [M+1]+

    Example 83: N1-(3-methyl)piperidine-N5-(3-trifluoromethoxy)phenyl biguanide hydrochloride



    [0218] 



    [0219] 1H NMR (600 MHz, CD3OD) δ 7.54 (s, 1H), 7.38 (t, 1H), 7.26 (d, 1H), 6.98 (d, 1H), 3.97 (s, 2H), 3.00 (s, 1H), 2.70 (t, 1H), 1.88 (m, 1H), 1.78-1.71 (m, 2H), 1.59 (m, 1H), 1.24 (q, 1H), 0.91 (d, 3H); LC-MS m/z 344.2 [M+1]+

    Example 84: N1-(3-methyl)piperidine-N5-(4-trifluoromethoxy)benzyl biguanide hydrochloride



    [0220] 



    [0221] 1H NMR (600 MHz, CD3OD) δ 7.42 (d, 2H), 7.25 (d, 2H), 4.41 (s, 2H), 3.84 (dd, 2H), 2.85 (t, 1H), 2.52 (t, 1H), 1.82 (m, 1H), 1.66-1.55 (m, 2H), 1.47 (m, 1H), 1.16 (q, 1H), 0.84 (d, 3H); LC-MS m/z 358.2 [M+1]+

    Example 85: N1-(3-methyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0222] 



    [0223] 1H NMR (600 MHz, CD3OD) δ 7.84 (dd, 1H), 7.58 (dt, 1H), 7.29 (t, 1H), 3.95 (s, 2H), 2.99 (s, 1H), 2.96 (t, 1H), 1.88 (m, 1H), 1.77 (m, 1H), 1.69 (m, 1H), 1.57 (m, 1H), 1.24 (q, 1H), 0.94 (d, 3H); LC-MS m/z 346.2 [M+1]+

    Example 86: N1-(3-methyl)piperidine-N5-(4-trifluoromethyl)benzyl biguanide hydrochloride



    [0224] 



    [0225] 1H NMR (600 MHz, CD3OD) δ 7.64 (d, 2H), 7.51 (d, 2H), 4.47 (s, 2H), 3.84 (dd, 2H), 2.85 (t, 1H), 2.52 (t, 1H), 1.82 (m, 1H), 1.66 (m, 1H), 1.55 (m, 1H), 1.47 (m, 1H), 1.17 (q, 1H), 0.84 (d, 3H); LC-MS m/z 342.2 [M+1]+

    Example 87: N1-(3-methyl)piperidine-N5-(4-chloro)phenyl biguanide hydrochloride



    [0226] 



    [0227] 1H NMR (600 MHz, CD3OD) δ 7.35 (m, 4H), 3.96 (m, 2H), 3.00 (m, 1H), 2.67 (m, 1H), 1.89 (m, 1H), 1.68 (m, 3H) 1.24 (m, 1H), 0.94 (m, 3H); LC-MS m/z 294.2 [M+1] +; mp 252-254°C

    Example 88: N1-(3-methyl)piperidine-N5-(3-trifluoromethyl)phenyl biguanide hydrochloride



    [0228] 



    [0229] 1H NMR (600 MHz, CD3OD) δ 7.86 (m, 1H), 7.55 (d, 1H), 7.48 t, 1H), 7.37 (d, 1H), 3.97 (s, 2H), 3.01 (t, 1H), 2.71 (t, 1H), 1.89 (m, 1H), 1.77(m, 1H), 1.70 (m, 1H), 1.59 (m, 1H), 1.25 (q, 1H), 0.94 (d, 3H); LC-MS m/z 328.2 [M+1]+

    Example 89: N1-(2,6-dimethyl)piperidine-N5-(4-trifluoromethoxy)phenyl biguanide hydrochloride



    [0230] 



    [0231] 1H NMR (600 MHz, CD3OD) δ 7.46 (d, 2H), 7.23 (d, 2H), 4.60-4.10 (br s, 2H), 1.90-1.51 (m, 6H), 1.30 (s, 6H); LC-MS m/z 358.4 [M+1]+

    Example 90: N1-(2,6-dimethyl)piperidine-N5-(3-trifluoromethoxy)phenyl biguanide hydrochloride



    [0232] 



    [0233] 1H NMR (600 MHz, CD3OD) δ 7.54 (s, 1H), 7.37 (t, 1H), 7.25 (d, 1H), 6.97 (d, 1H), 4.60-4.10 (br s, 2H), 1.91-1.52 (m, 6H), 1.31 (s, 6H); LC-MS m/z 358.4 [M+1]+

    Example 91: N1-(2,6-dimethyl)piperidine-N5-(4-trifluoromethyl)phenyl biguanide hydrochloride



    [0234] 



    [0235] 1H NMR (600 MHz, CD3OD) δ 7.58 (s, 4H), 4.60-4.10 (br s, 2H), 1.93-1.52 (m, 6H), 1.32 (s, 6H); LC-MS m/z 342.4 [M+1]+

    Example 92: N1-(2,6-dimethyl)piperidine-N5-(3-trifluoromethyl)phenyl biguanide hydrochloride



    [0236] 



    [0237] 1H NMR (600 MHz, CD3OD) δ 7.87 (s, 1H), 7.54 (d, 1H), 7.48 (t, 1H), 7.36 (d, 1H), 4.60-4.10 (br s, 2H), 1.93-1.52 (m, 6H), 1.37 (s, 6H); LC-MS m/z 342.4 [M+1]+

    Example 93: N1-(2,6-dimethyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0238] 



    [0239] 1H NMR (600 MHz, CD3OD) δ 7.83 (, d, 1H), 7.57 (m, 1H), 7.28 (t, 1H), 4.60-4.10 (br s, 2H), 1.93-1.51 (m, 6H), 1.30 (s, 6H); LC-MS m/z 360.4 [M+1]+

    Example 94: N1-(2,6-dimethyl)piperidine-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide hydrochloride



    [0240] 



    [0241] 1H NMR (600 MHz, CD3OD) δ 7.96 (s, 1H), 7.54 (m, 2H), 4.60-4.10 (br s, 2H), 1.93-1.52 (m, 6H), 1.31 (s, 6H); LC-MS m/z 376.4 [M+1]+

    Example 95: N1-(2,6-dimethyl)piperidine-N5-(3-fluoro, 4-trifluoromethoxy)phenyl biguanide



    [0242] 



    [0243] 1H NMR (600 MHz, CD3OD) δ 7.56 (d, 1H), 7.33 (t, 1H), 7.15 (d, 1H), 4.60-4.10 (br s, 2H), 1.93-1.52 (m, 6H), 1.32 (s, 6H); LC-MS m/z 376.4 [M+1]+

    Example 96: N1-(2,6-dimethyl)piperidine-N5-(4-chloro)phenyl biguanide hydrochloride



    [0244] 



    [0245] 1H NMR (600 MHz, CD3OD) δ 7.36 (m, 2H), 7.30 (m, 2H), 4.60-4.10 (br s, 2H), 1.92-1.51 (m, 6H), 1.30 (s, 6H); LC-MS m/z 308.2, 310.2 [M+1]+

    Example 97: N1-(2,6-dimethyl)piperidine-N5-(4-bromo)phenyl biguanide hydrochloride



    [0246] 



    [0247] 1H NMR (600 MHz, CD3OD) δ 7.45 (d, 2H), 7.31 (d, 2H), 4.60-4.10 (br s, 2H), 1.93-1.51 (m, 6H), 1.30 (s, 6H); LC-MS m/z 353.2 [M+1]+

    Example 98: N1-(2,6-dimethyl)piperidine-N5-(4-fluoro)phenyl biguanide hydrochloride



    [0248] 



    [0249] 1H NMR (600 MHz, CD3OD) δ 7.37 (m, 2H), 7.05 (m, 2H), 4.60-4.10 (br s, 2H), 1.88-1.50 (m, 6H), 1.29 (s, 6H); LC-MS m/z 292.1 [M+1]+

    [EXPERIMENTAL EXAMPLES]



    [0250] The compounds synthesized by the methods described in the examples of the present invention were evaluated for effects of AMPK activation and inhibition of cancer cell proliferation according to methods described in the following Experimental Examples.

    Experimental Example 1: Measurement of AMPK activation effect



    [0251] MCF7 cells derived from human breast cancer cells (purchased from the Korean Cell Line Bank (KCLB)) were used, and AMPK activation effects of the biguanide derivatives were confirmed using an AMPK [pT172] ELISA kit (Invitrogen, Catalog No. KHO0651).

    [0252] MCF7 cells were cultured in a DMEM medium supplemented with 10% fatal bovine serum (commercially available from Gibco Life Technologies (US)). Thereafter, the cultured MCF7 cells were put into a 6-well plate with approximately 5x105 cells per well, and cultured in an incubator supplied with 5% CO2. Culture media were treated with the derivatives synthesized in the Examples at concentrations of 5, 10 and 50 u M, and then cultured for 24 hours. Metformin was used as the control, and the culture media were treated with 0.05, 0.5, 1, 2, 5 and 10 mM metformin and then tested in the same manner as described for the derivatives synthesized in the Examples. Subsequently, the cells were lysed according to a method presented in the operation manual of the AMPK [pT172] ELISA kit, and 20µg of a cell lysate was then yielded through protein assay. Thereafter, the AMPK activation effect was obtained by determining a degree of phosphorylation of a 172nd threonine residue (Thr172) of the AMPKα from the cell lysate according to the method presented in the operation manual of the AMPK [pT172] ELISA kit. A degree of AMPK activation by the biguanide derivatives was exhibited as a degree of AMPKα phosphorylation in cells cultured in the presence of the compounds synthesized in the Examples with respect to a degree of AMPKα phosphorylation in cells cultured without treatment with the biguanide derivatives. A curve graph showing AMPK activation according to the concentration of the treated compounds was plotted based on the obtained AMPK activation results, and a concentration (activation concentration 150, AC150) value of a compound whose AMPK activation reached 150% was calculated using a GraphPad Prism 5.0 program. Similarly, degrees of AMPK activation when the concentrations of the treated biguanide derivatives of Examples 4 to 61 were 10µM and 50µM are listed in the following Table 1, and degrees of AMPK activation when the concentrations of the treated biguanide derivatives of Examples 65 to 98 were 5µM to 10µM are listed in the following Table 2. Some of the compounds were not measurable for AMPK activation due to cytotoxicity when the concentrations of the treated compounds were 50µM.

    Table 1



    [0253] 
    [Table 1]
    ExampleAMPK activation effect (%)
    AC150 (µM)10 µM50 µM
    Metformin 188.3 ND 130
    4 5.0 201 584
    5 2.8 235 223
    6 >50 124 136
    7 >50 94 109
    8 8.3 139 515
    9 9.0 169 371
    10 1.4 408 -
    11 1.4 637 -
    12 13.8 97 357
    13 9.0 135 462
    14 8.4 1.51 507
    15 1.6 554 -
    16 1-7 398 -
    17 4-7 208 -
    18 0.81 - -
    19 1.5 306 -
    20 38.3 83 172
    21 11.9 156 -
    22 24.5 116 209
    23 213 57 279
    24 53 200 421
    25 2.2 376 -
    26 0.68 443 358
    27 13.5 119 316
    28 0.15 732 -
    29 1.3 551 -
    30 0.53 703 -
    31 1.8 391 -
    32 0.62 837 -
    33 0.79 992 -
    34 0.29 925 -
    35 2.4 314 -
    36 0.87 568 -
    37 4.2 187 522
    38 >50 97 117
    39 67.2 96 138
    40 37.8 122 163
    41 22.4 125 201
    42 14.7 157 210
    43 4.7 200 466
    44 1.7 289 577
    45 4.8 202 299
    46 1.6 448 -
    47 1.0 758 -
    48 4.9 178 691
    49 1.9 319 917
    50 1.4 317 440
    51 3.1 241 409
    52 2.7 352 -
    53 2.3 311 589
    54 0.87 545 650
    55 2.1 389 608
    56 2.1 389 671
    57 2.7 279 579
    58 1.8 500 -
    59 16.6 104 283
    60 12.4 108 338
    61 >50 123 132

    Table 2



    [0254] 
    [Table 2]
    ExampleAMPK activation effect (%)
     AC150 (µM)5 µM10 µM
    Metformin 188.3   130 (at 50 µM)
    65 2.8 147 274
    66 6.2 101 204
    67 1.6 258 427
    68 13.7 52 136
    69 1.2 307 650
    70 0.9 365 602
    71 1.6 259 430
    72 2.0 210 386
    73 2.0 215 384
    74 2.0 199 410
    75 3.9 133 259
    76 1.7 242 375
    77 4.5 154 212
    78 0.5 425 565
    79 1.9 177 515
    80 1.1 334 590
    81 1.9 224 396
    82 0.5 401 474
    83 0.5 352 398
    84 1.1 317 555
    85 0.7 345 436
    86 2.3 147 330
    87 1.9 219 297
    88 0.7 327 399
    89 4.7 153 198
    90 0.8 281 317
    91 5.6 108 214
    92 0.8 324 417
    93 2.0 190 422
    94 1.1 328 643
    95 3.1 130 326
    96 1.8 240 395
    97 4.2 143 233
    98 2.8 239 244

    Experimental Example 2: Measurement of effect of inhibiting cancer cell proliferation



    [0255] HCT116 cells derived from human colorectal cancer were used, and an effect of inhibiting cancer cell proliferation of the biguanide derivative was confirmed by measuring a concentration value (cell growth inhibition concentration, GIC50) at which cell growth was inhibited by 50% using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) reagent.

    [0256] First, HCT116 cells (purchased from the KCLB) were put on a 96-well plate and cultured in a DMEM medium supplemented with 10% fatal bovine serum (commercially available from Gibco Life Technologies (US)) for 16 hours so that approximately 5,000 cells were counted in each well. Subsequently, to obtain the GIC50 value of each compound, the culture media were treated with 100µM, 25µM, 6.25µM, 1.56µM or 0.39µM of the compound, and then cultured for 48 hours. In order to determine whether the cells survived after treatment with the compounds, MTT (commercially available from AMRESCO (US)) was added to the culture media which were then cultured for another 3 hours. Formed formazane crystals were dissolved using dimethyl sulfoxide (DMSO) and the absorbance of the resulting solution was measured at 560 nm. After the 48-hour culture, a ratio of a living cell count on a well plate treated with the compounds synthesized in the Examples to a cell count cultured on a well plate not treated with the compound was indicated as cell viability (%) according to the concentration of each treated compound. The cell viability (%) was used to plot a cell viability curve graph and calculate a concentration (GIC50) value of the compound at which the growth was inhibited by 50%, thereby confirming the inhibition effect of cancer cell proliferation. Also, the cell growth inhibitions (%) when the concentration of the treated biguanide derivative and metformin as the control was 100µM (Examples 4 to 61) is listed in the following Table 3, and the cell growth inhibitions (%) when the concentration of the treated biguanide derivative and metformin as the control was 25µM (Examples 65 to 98) are listed in the following Table 4.

    Table 3



    [0257] 
    [Table 3]
    ExamplesEffect of inhibition on cancer cell growth
    GI50 (µM)Cell growth inhibition (%) at 100 µM
    Metformin 2172 6.5 (at 100 µM)
    4 19.1 99.7
    5 18.0 99.7
    6 12.9 99.6
    7 >100 19.8
    8 42.8 99.1
    9 28.8 99.5
    10 8.3 97.9
    11 3.0 98.0
    12 84.8 53.8
    13 77.1 57.8
    14 17.5 98.8
    15 10.1 99.2
    16 10.8 99.3
    17 13.9 99.7
    18 2.6 99.5
    19 9.1 99.6
    20 54.8 93.1
    21 10.4 99.4
    22 18.6 37.1
    23 89.9 29.5
    24 33.9 99.0
    25 4.9 96.6
    26 8.2 96.7
    27 33.5 96.6
    28 8.4 100.5
    29 9.5 100.4
    30 7.5 99.7
    31 7.8 99.5
    32 7.9 99.4
    33 3.7 99.4
    34 6.6 99.4
    35 18.4 99.1
    36 11.2 99.0
    37 135.0 45.3
    38 108.5 47.8
    39 123.7 39.8
    40 >100 37.4
    41 88.2 55.9
    42 >100 14.0
    43 114.4 44.9
    43 114.4 44.9
    44 >100 27.8
    45 107.2 47.2
    46 12.0 100.1
    47 10.8 100.0
    48 42.5 97 6
    49 57.0 91.8
    50 116.8 46.0
    51 102.5 48.7
    52 15.4 100.1
    53 43.7 97.9
    54 14.6 98.1
    55 22.8 97.9
    56 26.9 97.7
    57 26.9 97.8
    58 7.9 98.4
    59 141.4 44.5
    60 99.8 50.1
    61 >100 10.5

    Table 4



    [0258] 
    [Table 4]
    ExamplesEffect of inhibition on cancer cell growth
    GI50 (µM)Cell growth inhibition (%) at 25 µM
    Metformin 2172 6.5 (at 100 µM)
    65 18.3 66.7
    66 18.5 67.4
    67 9.2 100.2
    68 45.3 29.4
    69 1.9 100.5
    70 3.9 100.5
    71 5.7 100.4
    72 8.0 100.3
    73 6.7 100.3
    74 8.7 100.4
    75 9.3 100.3
    76 5.8 100.4
    77 11.3 100.2
    78 7.0 100.3
    79 9.4 98.9
    80 6.8 99.7
    81 7.7 100.3
    82 7.0 100.3
    83 6.8 100.2
    84 10.4 97.0
    85 7.1 100.2
    86 27.6 49.0
    87 10.0 100.1
    88 6.6 100.4
    89 6.2 100.4
    90 3.2 100.4
    91 11.4 100.4
    92 7.5 100.4
    93 3.0 100.3
    94 2.8 100.5
    95 2.9 100.6
    96 10.4 100.6
    97 9.1 100.5
    98 45.4 28.2



    Claims

    1. A compound of following Formula 1 or a pharmaceutically acceptable salt thereof:

    wherein R1 and R2 are taken together with nitrogen to which they are attached to form C3-6 heterocycloalkene having 4 to 7 ring atoms; or piperidinyl, which is substituted with C1-4 alkyl in at least one position thereof; and

    R3 is hydrogen; C1-6 alkyl; phenyl; or C1-4 alkyl substituted with phenyl,

    wherein the C3-6 heterocycloalkene is unsubstituted or substituted with at least one substituent selected from the group consisting of halogen, hydroxy and C1-6 alkyl,

    the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, and C1-6 haloalkoxy.


     
    2. The compound of Formula 1 or the pharmaceutically acceptable salt thereof of claim 1,
    wherein R1 and R2 are taken together with nitrogen to which they are attached to form C4-5 heterocycloalkene having 5 to 6 ring atoms or piperidinyl, which is substituted with C1-4 alkyl in at least one position thereof; and
    R3 is hydrogen; C1-6 alkyl; phenyl; or C1-2 alkyl substituted with phenyl,
    wherein the C4-5 heterocycloalkene is unsubstituted or substituted with C1-6 alkyl, and
    the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, and C1-6 haloalkoxy.
     
    3. The compound of Formula 1 or the pharmaceutically acceptable salt thereof of claim 1,
    wherein R1 and R2 are taken together with nitrogen to which they are attached to form C4-5 heterocycloalkene having 5 to 6 ring atoms or piperidinyl, which is substituted with one or two C1-2 alkyl groups; and
    R3 is hydrogen; C1-6 alkyl; phenyl; or C1-2 alkyl substituted with phenyl,
    wherein the C4-5 heterocycloalkene is unsubstituted or substituted with C1-2 alkyl, and
    the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, C1-2 alkoxy, C1-2 haloalkyl, and C1-2 haloalkoxy.
     
    4. The compound of Formula 1 or the pharmaceutically acceptable salt thereof of
    claim 1,
    wherein R1 and R2 are taken together with nitrogen to which they are attached to form C3-6 heterocycloalkene selected from the group consisting of dihydroazetinyl; dihydropyrrolinyl; dihydropyridinyl; and tetrahydropyridinyl, or R1 and R2 are taken together with nitrogen to which they are attached to form piperidinyl, which is substituted with C1-4 alkyl in at least one position thereof; and
    R3 is hydrogen; C1-6 alkyl; phenyl; or C1-4 alkyl substituted with phenyl,
    wherein the C3-6 heterocycloalkene is unsubstituted or substituted with C1-6 alkyl, and
    the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, and C1-6 haloalkoxy.
     
    5. The compound of Formula 1 or the pharmaceutically acceptable salt thereof of
    claim 1,
    wherein R1 and R2 are taken together with nitrogen to which they are attached to form dihydropyrrolinyl or tetrahydropyridinyl which is unsubstituted or substituted with C1-4 alkyl, or R1 and R2 are taken together with nitrogen to which they are attached to form piperidinyl which is substituted with C1-4 alkyl in at least one position thereof; and
    R3 is hydrogen; C1-6 alkyl; phenyl; or C1-4 alkyl substituted with phenyl,
    wherein the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, C1-4 alkoxy, C1-4 haloalkyl, and C1-4 haloalkoxy.
     
    6. The compound of Formula 1 or the pharmaceutically acceptable salt thereof of
    claim 1,
    wherein R1 and R2 are taken together with nitrogen to which they are attached to form dihydropyrrolinyl or tetrahydropyridinyl which is unsubstituted or substituted with C1-2 alkyl, or R1 and R2 are taken together with nitrogen to which they are attached to form piperidinyl which is substituted with one or two C1-2 alkyl groups; and
    R3 is hydrogen; C3-6 alkyl; phenyl; or C1-2 alkyl substituted with phenyl,
    wherein the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, C1-2 alkoxy, C1-2 haloalkyl, and C1-2 haloalkoxy.
     
    7. The compound of Formula 1 or the pharmaceutically acceptable salt thereof of
    claim 1,
    wherein R1 and R2 are taken together with nitrogen to which they are attached to form dihydropyrrolinyl or tetrahydropyridinyl which is unsubstituted or substituted with methyl, or R1 and R2 are taken together with nitrogen to which they are attached to form piperidine; and
    R3 is hydrogen; butyl; propyl; hexyl; phenyl; or methyl substituted with phenyl,
    wherein the piperidine is substituted with one or two methyl groups in at least one of positions 2, 3, 5 and 6, and
    the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, methoxy, trihalomethyl, and trihalomethoxy.
     
    8. The compound of Formula 1 or the pharmaceutically acceptable salt thereof of
    claim 1, wherein the compound of Formula 1 is

    N1-1,2-dihydropyrrole-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-fluoro)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-chloro)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-bromo)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-chloro, 4-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3 ,6-tetrahydropyridine-N5-(3-chloro, 4-trifluoromethoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-trifluoromethyl)benzyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-trifluoromethyl)benzyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-trifluoromethoxy)benzyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-chloro)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-fluoro)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-bromo)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-methoxy)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(3,4-dimethoxy)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethoxy)benzyl biguanide;

    Nl-(3-methyl)-1,2-dihydropyrrole-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-trifluoromethyl)benzyl biguanide;

    N1-(3-methyl)-1,2-dihydropyrrole-N5-(4-chloro, 3-trifluoromethyl)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-chloro, 3-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-fluoro,4-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-chloro)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-bromo)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-fluoro)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3,5-dimethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-methoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-methoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-methoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-methoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3,5-dimethoxy)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-fluoro, 4-trifluoromethyl)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-methyl)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-methyl)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(4-methyl)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-methyl)phenyl biguanide;

    N1-1,2-dihydropyrrole-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-hexyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethoxy)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethoxy)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-trifluoromethyl)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(3-trifluoromethyl)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-(4-chloro, 3-trifluoromethyl)benzyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-butyl biguanide;

    N1-1,2,3,6-tetrahydropyridine-N5-propyl biguanide;

    N1-1,2,3,6-tetrahydropyridine biguanide;

    N1-(3-methyl)piperidine-N5-(3-trifluoromethyl)benzyl biguanide;

    Nl-(3-methyl)piperidine-N5-(4-chloro)benzyl biguanide;

    Nl-(3-methyl)piperidine-N5-(4-fluoro)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-bromo)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(3-fluoro, 4-trifluoromethyl)phenyl biguanide;

    Nl-(3-methyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(3-fluoro, 4-trifluoromethoxy)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(3-fluoro, 4-trifluoromethoxy)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(4-chloro)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-(2-methyl)piperidine-N5-(4-chloro, 3-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-trifluoromethoxy)phenyl biguanide;

    Nl-(3-methyl)piperidine-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-trifluoromethoxy)benzyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-trifluoromethyl)benzyl biguanide;

    N1-(3-methyl)piperidine-N5-(4-chloro)phenyl biguanide;

    N1-(3-methyl)piperidine-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-trifluoromethoxy)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(3-trifluoromethoxy)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-trifluoromethyl)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(3-trifluoromethyl)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-fluoro, 3-trifluoromethyl)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-chloro, 3 -trifluoromethyl)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(3-fluoro, 4-trifluoromethoxy)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-chloro)phenyl biguanide;

    N1-(2,6-dimethyl)piperidine-N5-(4-bromo)phenyl biguanide; and

    N1-(2,6-dimethyl)-N5-(4-fluoro)phenyl biguanide.


     
    9. The compound of Formula 1 or the pharmaceutically acceptable salt thereof of claim 1,
    wherein the pharmaceutically acceptable salt is a salt with an acid selected from the group consisting of formic acid, acetic acid, propionic acid, lactic acid, butyric acid, isobutyric acid, trifluoroacetic acid, malic acid, maleic acid, malonic acid, fumaric acid, succinic acid, succinic acid monoamide, glutamic acid, tartaric acid, oxalic acid, citric acid, glycolic acid, glucuronic acid, ascorbic acid, benzoic acid, phthalic acid, salicylic acid, anthranilic acid, benzensulfonic acid, p-toluenesulfonic acid, methanesulfonic acid, dichloroacetic acid, aminooxy acetic acid, hydrochloric acid, hydrobromic acid, sulfuric acid, phosphoric acid, nitric acid, carbonic acid, and boric acid.
     
    10. A method of preparing a compound of following Formula 1, comprising:

    reacting a compound of the following Formula 2 with a dicyanamide in an organic solvent to obtain a compound of the following Formula 3; and

    reacting the compound of the following Formula 3 with a compound of Formula 4 in an organic solvent to obtain the compound of the following Formula 1:







    wherein R1 and R2 are taken together with nitrogen to which they are attached to form C3-6 heterocycloalkene having 4 to 7 ring atoms or piperidinyl, which is substituted with C1-4 alkyl in at least one position thereof; and

    R3 is hydrogen; C1-6 alkyl; phenyl; or C1-4 alkyl substituted with phenyl,

    wherein the C3-6 heterocycloalkene is unsubstituted or substituted with at least one substituent selected from the group consisting of halogen, hydroxyl, and C1-6 alkyl, and

    the phenyl is unsubstituted or substituted with at least one non-hydrogen substituent selected from the group consisting of halogen, hydroxy, C1-6 alkyl, C1-6 alkoxy, C1-6 haloalkyl, and C1-6 haloalkoxy.


     
    11. A pharmaceutical composition for use in preventing or treating a cancer, comprising the compound of Formula 1 or the pharmaceutically acceptable salt thereof defined in any one of claims 1 to 9 as an active ingredient.
     
    12. The pharmaceutical composition for use of claim 11, wherein the treatment of the cancer includes inhibition of recurrence or metastasis of cancer.
     
    13. The pharmaceutical composition for use of claim 11, wherein the cancer is a disease selected from the group consisting of uterine cancer, breast cancer, gastric cancer, brain cancer, rectal cancer, colorectal cancer, lung cancer, skin cancer, blood cancer, pancreatic cancer, prostate cancer, and liver cancer.
     
    14. The pharmaceutical composition for use of claim 11, wherein the pharmaceutical composition is formulated as a tablet, a capsule, a pill, a granule, a powder, an injection or a liquid.
     


    Ansprüche

    1. Eine Verbindung der nachstehenden Formel 1 oder ein pharmazeutisch verträgliches Salz davon:

    wobei R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um C3-6-Heterocycloalken mit 4 bis 7 Ringatomen oder Piperidinyl, das mit C1-4-Alkyl an mindestens einer Position davon substituiert ist, zu bilden; und

    R3 Wasserstoff; C1-6-Alkyl; Phenyl oder mit Phenyl substituiertes C1-4-Alkyl ist, wobei das C3-6-Heterocycloalken unsubstituiert oder mit mindestens einem Substituenten, der ausgewählt ist aus der Gruppe bestehend aus Halogen, Hydroxy und C1-6-Alkyl substituiert ist,

    das Phenyl unsubstituiert oder mit mindestens einem Nichtwasserstoffsubstituenten, der ausgewählt ist aus der Gruppe bestehend aus Halogen, Hydroxy, C1-6-Alkyl, C1-6-Alkoxy, C1-6-Halogenalkyl und C1-6-Halogenalkoxy, substituiert ist.


     
    2. Die Verbindung der Formel 1 oder das pharmazeutisch verträgliche Salz davon nach Anspruch 1,
    wobei R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um C4-5-Heterocycloalken mit 5 bis 6 Ringatomen oder Piperidinyl, das mit C1-4-Alkyl an mindestens einer Position davon substituiert ist, zu bilden; und
    R3 Wasserstoff; C1-6-Alkyl; Phenyl oder mit Phenyl substituiertes C1-2-Alkyl ist, wobei das C4-5-Heterocycloalken unsubstituiert oder mit C1-6-Alkyl substituiert ist und
    das Phenyl unsubstituiert oder mit mindestens einem Nichtwasserstoffsubstituenten, der ausgewählt ist aus der Gruppe bestehend aus Halogen, Hydroxy, C1-6-Alkyl, C1-6-Alkoxy, C1-6-Halogenalkyl und C1-6-Halogenalkoxy, substituiert ist.
     
    3. Die Verbindung der Formel 1 oder das pharmazeutisch verträgliche Salz davon nach Anspruch 1,
    wobei R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um C4-5-Heterocycloalken mit 5 bis 6 Ringatomen oder Piperidinyl, das mit einer oder zwei C1-2-Alkylgruppen substituiert ist, zu bilden; und
    R3 Wasserstoff; C1-6-Alkyl; Phenyl oder mit Phenyl substituiertes C1-2-Alkyl ist, wobei das C4-5-Heterocycloalken unsubstituiert oder mit C1-2-Alkyl substituiert ist und
    das Phenyl unsubstituiert oder mit mindestens einem Nichtwasserstoffsubstituenten, der ausgewählt ist aus der Gruppe bestehend aus Halogen, C1-2-Alkoxy, C1-2-Halogenalkyl und C1-2-Halogenalkoxy, substituiert ist.
     
    4. Die Verbindung der Formel 1 oder das pharmazeutisch verträgliche Salz davon nach Anspruch 1,
    wobei R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um C3-6-Heterocycloalken zu bilden, das ausgewählt ist aus der Gruppe bestehend aus Dihydroazetinyl; Dihydropyrrolinyl; Dihydropyridinyl und Tetrahydropyridinyl, oder R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um Piperidinyl zu bilden, das mit C1-4-Alkyl an mindestens einer Position davon substituiert ist; und
    R3 Wasserstoff; C1-6-Alkyl; Phenyl oder mit Phenyl substituiertes C1-4-Alkyl ist, wobei das C3-6-Heterocycloalken unsubstituiert oder mit C1-6-Alkyl substituiert ist und
    das Phenyl unsubstituiert oder mit mindestens einem Nichtwasserstoffsubstituenten, der ausgewählt ist aus der Gruppe bestehend aus Halogen, Hydroxy, C1-6-Alkyl, C1-6-Alkoxy, C1-6-Halogenalkyl und C1-6-Halogenalkoxy, substituiert ist.
     
    5. Die Verbindung der Formel 1 oder das pharmazeutisch verträgliche Salz davon nach Anspruch 1,
    wobei R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um Dihydropyrrolinyl oder Tetrahydropyridinyl, das unsubstituiert oder mit C1-4-Alkyl substituiert ist, zu bilden, oder R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um Piperidinyl, das mit C1-4-Alkyl an mindestens einer Position davon substituiert ist, zu bilden; und
    R3 Wasserstoff; C1-6-Alkyl; Phenyl oder mit Phenyl substituiertes C1-4-Alkyl ist, wobei das Phenyl unsubstituiert oder mit mindestens einem Nichtwasserstoffsubstituenten, der ausgewählt ist aus der Gruppe bestehend aus Halogen, C1-4-Alkoxy, C1-4-Halogenalkyl und C1-4-Halogenalkoxy, substituiert ist.
     
    6. Die Verbindung der Formel 1 oder das pharmazeutisch verträgliche Salz davon nach Anspruch 1,
    wobei R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um Dihydropyrrolinyl oder Tetrahydropyridinyl, das unsubstituiert oder mit C1-2-Alkyl substituiert ist, zu bilden, oder R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um Piperidinyl, das mit einer oder zwei C1-2-Alkylgruppen substituiert ist, zu bilden; und
    R3 Wasserstoff; C3-6-Alkyl; Phenyl oder mit Phenyl substituiertes C1-2-Alkyl ist, wobei das Phenyl unsubstituiert oder mit mindestens einem Nichtwasserstoffsubstituenten, der ausgewählt ist aus der Gruppe bestehend aus Halogen, C1-2-Alkoxy, C1-2-Halogenalkyl und C1-2-Halogenalkoxy, substituiert ist.
     
    7. Die Verbindung der Formel 1 oder das pharmazeutisch verträgliche Salz davon nach Anspruch 1,
    wobei R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um Dihydropyrrolinyl oder Tetrahydropyridinyl, das unsubstituiert oder mit Methyl substituiert ist, zu bilden, oder R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um Piperidin zu bilden; und
    R3 Wasserstoff; Butyl; Propyl; Hexyl; Phenyl oder mit Phenyl substituiertes Methyl ist,
    wobei das Piperidin mit einer oder zwei Methylgruppen an mindestens einer der Positionen 2, 3, 5 und 6 substituiert ist und
    das Phenyl unsubstituiert oder mit mindestens einem Nichtwasserstoffsubstituenten, der ausgewählt ist aus der Gruppe bestehend aus Halogen, Methoxy, Trihalogenmethyl und Trihalogenmethoxy, substituiert ist.
     
    8. Die Verbindung der Formel 1 oder das pharmazeutisch verträgliche Salz davon nach Anspruch 1, wobei die Verbindung der Formel 1

    N1-1,2-Dihydropyrrol-N5-(4-trifluormethoxy)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(4-trifluormethyl)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(3-trifluormethyl)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(4-fluor)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(4-chlor)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(4-brom)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(3-chlor, 4-trifluormethoxy)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(3-chlor, 4-trifluormethoxy)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(3-trifluormethyl)benzylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(4-trifluormethyl)benzylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(3-trifluormethoxy)benzylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(4-trifluormethoxy)phenylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(4-trifluormethyl)phenylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(4-chlor)phenylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(4-chlor, 3-trifluormethyl)phenylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(3-trifluormethyl)phenylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(4-fluor)phenylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(4-brom)phenylbiguanid;

    Nl-(3-Methyl)-1,2-dihydropyrrol-N5-(4-methoxy)phenylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(3,4-dimethoxy)phenylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(4-trifluormethoxy)benzylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(3-trifluormethoxy)phenylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(4-trifluormethyl)benzylbiguanid;

    N1-(3-Methyl)-1,2-dihydropyrrol-N5-(4-chlor, 3-trifluormethyl)benzylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-trifluormethoxy)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-trifluormethyl)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(3-trifluormethoxy)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(3-trifluormethyl)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-fluor, 3-trifluormethyl)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-chlor, 3-trifluormethoxy)phenylbiguanid;

    N1-1,2,3,6- Tetrahydropyridin-N5-(3-fluor,4-trifluormethoxy)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-chlor)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-brom)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-fluor)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(3,5-dimethoxy)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-methoxy)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(3-methoxy)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(4-methoxy)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(3-methoxy)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(3,5-dimethoxy)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(4-fluor, 3-trifluormethyl)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(3-fluor, 4-trifluormethyl)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-methyl)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(3 -methyl)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(4-methyl)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(3-methyl)phenylbiguanid;

    N1-1,2-Dihydropyrrol-N5-(3-trifluormethoxy)phenylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-hexylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-trifluormethoxy)benzylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(3-trifluormethoxy)benzylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-trifluormethyl)benzylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(3-trifluormethyl)benzylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-N5-(4-chlor, 3-trifluormethyl)benzylbiguanid;

    N1-1,2,3,6-Tetrahydropyridin-NS-butyl biguanide;

    N1-1,2,3,6-Tetrahydropyridin-N5-propyl biguanide;

    N1-1,2,3,6- Tetrahydropyridinbiguanid;

    N1-(3-Methyl)piperidin-N5-(3-trifluormethyl)benzylbiguanid;

    N1-(3-Methyl)piperidin-N5-(4-chlor)benzylbiguanid;

    N1-(3-Methyl)piperidin-N5-(4-fluor)phenylbiguanid;

    N1-(3-Methyl)piperidin-N5-(4-brom)phenylbiguanid;

    N1-(3-Methyl)piperidin-N5-(4-chlor, 3-trifluormethyl)phenylbiguanid;

    N1-(3-Methyl)piperidin-N5-(3-fluor, 4-trifluormethyl)phenylbiguanid;

    N1-(3-Methyl)piperidin-N5-(4-fluor, 3-trifluormethyl)phenylbiguanid;

    N1-(2-Methyl)piperidin-N5-(4-trifluormethoxy)phenylbiguanid;

    N1-(2-Methyl)piperidin-N5-(3-trifluormethoxy)phenylbiguanid;

    N1-(2-Methyl)piperidin-N5-(4-trifluormethyl)phenylbiguanid,

    Nl-(3-Methyl)piperidin-N5-(3-fluor, 4-trifluormethoxy)phenylbiguanid;

    N1-(2-Methyl)piperidin-N5-(3-fluor, 4-trifluormethoxy)phenylbiguanid;

    N1-(2-Methyl)piperidin-N5-(4-chlor)phenylbiguanid;

    N1-(2-Methyl)piperidin-N5-(4-fluor, 3-trifluormethyl)phenylbiguanid;

    N1-(2-Methyl)piperidin-N5-(3-trifluormethyl)phenylbiguanid;

    N1-(2-Methyl)piperidin-N5-(4-chlor, 3-trifluormethyl)phenylbiguanid;

    N1-(3-Methyl)piperidin-N5-(4-trifluormethyl)phenylbiguanid;

    N1-(3-Methyl)piperidin-N5-(4-trifluormethoxy)phenylbiguanid;

    N1-(3-Methyl)piperidin-N5-(3-trifluormethoxy)phenylbiguanid;

    N1-(3-Methyl)piperidin-N5-(4-trifluormethoxy)benzylbiguanid;

    N1-(3-Methyl)piperidin-N5-(4-fluor, 3-trifluormethyl)phenylbiguanid;

    Nl-(3-Methyl)piperidin-N5-(4-trifluormethyl)benzylbiguanid;

    N1-(3-Methyl)piperidin-N5-(4-chlor)phenylbiguanid;

    N1-(3-Methyl)piperidin-N5-(3-trifluormethyl)phenylbiguanid;

    N1-(2,6-Dimethyl)piperidin-N5-(4-trifluormethoxy)phenylbiguanid;

    N1-(2,6-Dimethyl)piperidin-N5-(3-trifluormethoxy)phenylbiguanid,

    N1-(2,6-Dimethyl)piperidin-N5-(4-trifluormethyl)phenylbiguanid;

    N1-(2,6-Dimethyl)piperidin-N5-(3-trifluormethyl)phenylbiguanid,

    N1-(2,6-Dimethyl)piperidin-N5-(4-fluor, 3-trifluormethyl)phenylbiguanid;

    N1-(2,6-Dimethyl)piperidin-N5-(4-chlor, 3-trifluormethyl)phenylbiguanid;

    N1-(2,6-Dimethyl)piperidin-N5-(3-fluor, 4-trifluormethoxy)phenylbiguanid;

    N1-(2,6-Dimethyl)piperidin-N5-(4-chlor)phenylbiguanid;

    N1-(2,6-Dimethyl)piperidin-N5-(4-brom)phenylbiguanid; und

    N1-(2,6-Dimethyl)-N5-(4-fluor)phenylbiguanid.


     
    9. Die Verbindung der Formel 1 oder das pharmazeutisch verträgliche Salz davon nach Anspruch 1,
    wobei das pharmazeutisch verträgliche Salz ein Salz mit einer Säure ist, die ausgewählt ist aus der Gruppe bestehend aus Ameisensäure, Essigsäure, Propionsäure, Milchsäure, Buttersäure, Isobuttersäure, Trifluoressigsäure, Äpfelsäure, Maleinsäure, Malonsäure, Fumarsäure, Bernsteinsäure, Bernsteinsäuremonoamid, Glutaminsäure, Weinsäure, Oxalsäure, Zitronensäure, Glycolsäure, Glucuronsäure, Ascorbinsäure, Benzoesäure, Phthalsäure, Salicylsäure, Anthranilsäure, Benzensulfonsäure, p-Toluolsulfonsäure, Methansulfonsäure, Dichloressigsäure, Aminooxyessigsäure, Salzsäure, Bromwasserstoffsäure, Schwefelsäure, Phosphorsäure, Salpetersäure, Kohlensäure und Borsäure.
     
    10. Ein Verfahren zur Herstellung einer Verbindung der nachstehenden Formel 1, umfassend:

    Umsetzen einer Verbindung der nachstehenden Formel 2 mit einem Dicyanamid in einem organischen Lösungsmittel, um eine Verbindung der nachstehenden Formel 3 zu erhalten; und

    Umsetzen einer Verbindung der nachstehenden Formel 3 mit einer Verbindung von Formel 4 in einem organischen Lösungsmittel, um eine Verbindung der nachstehenden Formel 1 zu erhalten:







    wobei R1 und R2 mit dem Stickstoff, an das sie gebunden sind, zusammengenommen werden, um C3-6-Heterocycloalken mit 4 bis 7 Ringatomen oder Piperidinyl, das mit C1-4-Alkyl an mindestens einer Position davon substituiert ist, zu bilden; und

    R3 Wasserstoff; C1-6-Alkyl; Phenyl oder mit Phenyl substituiertes C1-4-Alkyl ist, wobei das C3-6-Heterocycyloalken unsubstituiert oder mit mindestens einem Substituenten, der ausgewählt ist aus der Gruppe bestehend aus Halogen, Hydroxyl und C1-6-Alkyl, substituiert ist und

    das Phenyl unsubstituiert oder mit mindestens einem Nichtwasserstoffsubstituenten, der ausgewählt ist aus der Gruppe bestehend aus Halogen, Hydroxy, C1-6-Alkyl, C1-6-Alkoxy, C1-6-Halogenalkyl und C1-6-Halogenalkoxy, substituiert ist.


     
    11. Ein Arzneimittel zur Verwendung bei der Vorbeugung oder Behandlung von Krebs, umfassend die Verbindung der Formel 1 oder das pharmazeutisch verträgliche Salz davon, wie in einem der Ansprüche 1 bis 9 definiert, als Wirkstoff.
     
    12. Das Arzneimittel zur Verwendung nach Anspruch 11, wobei die Behandlung von Krebs die Hemmung des Wiederauftretens oder der Metastasierung von Krebs umfasst.
     
    13. Das Arzneimittel zur Verwendung nach Anspruch 11, wobei der Krebs eine Erkrankung ist, die ausgewählt ist aus der Gruppe bestehend aus Gebärmutterkrebs, Brustkrebs, Magenkrebs, Hirnkrebs, Rektumkrebs, Kolorektalkrebs, Lungenkrebs, Hautkrebs, Blutkrebs, Bauchspeicheldrüsenkrebs, Prostatakrebs und Leberkrebs.
     
    14. Das Arzneimittel zur Verwendung nach Anspruch 11, wobei das Arzneimittel als eine Tablette, eine Kapsel, eine Pille, ein Granulat, ein Pulver, eine Injektion oder eine Flüssigkeit formuliert ist.
     


    Revendications

    1. Composé de formule 1 qui suit ou un sel pharmaceutiquement acceptable de celui-ci :

    formule dans laquelle R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former un hétérocycloalcène en C3 à C6 ayant 4 à 7 atomes de cycle ; ou pipéridinyle, qui est substitué par alkyle en C1 à C4 sur au moins une position de celui-ci ; et

    R3 est un hydrogène ; alkyle en C1 à C6 ; phényle ; ou alkyle en C1 à C4 substitué par phényle,

    où l'hétérocycloalcène en C3 à C6 est non substitué ou substitué par au moins un substituant choisi dans le groupe constitué par un halogène, hydroxy et alkyle en C1 à C6,

    le phényle est non substitué ou substitué par au moins un substituant non-hydrogène choisi dans le groupe constitué par un halogène, hydroxy, alkyle en C1 à C6, alcoxy en C1 à C6, halogénoalkyle en C1 à C6, et halogénoalcoxy en C1 à C6.


     
    2. Composé de formule 1 ou son sel pharmaceutiquement acceptable selon la revendication 1, dans lequel
    R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former un hétérocycloalcène en C4 à C5 ayant 5 ou 6 atomes de cycle ou pipéridinyle, qui est substitué par alkyle en C1 à C4 sur au moins une position de celui-ci ; et
    R3 est un hydrogène ; alkyle en C1 à C6 ; phényle ; ou alkyle en C1 à C2 substitué par phényle,
    où l'hétérocycloalcène en C4 à C5 est non substitué ou substitué par alkyle en C1 à C6, et
    le phényle est non substitué ou substitué par au moins un substituant non-hydrogène choisi dans le groupe constitué par un halogène, hydroxy, alkyle en C1 à C6, alcoxy en C1 à C6, halogénoalkyle en C1 à C6, et halogénoalcoxy en C1 à C6.
     
    3. Composé de formule 1 ou son sel pharmaceutiquement acceptable selon la revendication 1, dans lequel
    R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former un hétérocycloalcène en C4 à C5 ayant 5 ou 6 atomes de cycle ou pipéridinyle, qui est substitué par un ou deux groupes alkyle en C1 à C2 ; et
    R3 est un hydrogène ; alkyle en C1 à C6 ; phényle ; ou alkyle en C1 à C2 substitué par phényle,
    où l'hétérocycloalcène en C4 à C5 est non substitué ou substitué par alkyle en C1 à C2, et
    le phényle est non substitué ou substitué par au moins un substituant non-hydrogène choisi dans le groupe constitué par un halogène, alcoxy en C1 à C2, halogénoalkyle en C1 à C2, et halogénoalcoxy en C1 à C2.
     
    4. Composé de formule 1 ou son sel pharmaceutiquement acceptable selon la revendication 1, dans lequel
    R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former un hétérocycloalcène en C3 à C6 choisi dans le groupe constitué par dihydroazétinyle ; dihydropyrrolinyle ; dihydropyridinyle ; et tétrahydropyridinyle, ou bien R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former pipéridinyle, qui est substitué par alkyle en C1 à C4 sur au moins une position de celui-ci ; et
    R3 est un hydrogène ; alkyle en C1 à C6 ; phényle ; ou alkyle en C1 à C4 substitué par phényle,
    où l'hétérocycloalcène en C3 à C6 est non substitué ou substitué par alkyle en C1 à C6, et
    le phényle est non substitué ou substitué par au moins un substituant non-hydrogène choisi dans le groupe constitué par un halogène, hydroxy, alkyle en C1 à C6, alcoxy en C1 à C6, halogénoalkyle en C1 à C6, et halogénoalcoxy en C1 à C6.
     
    5. Composé de formule 1 ou son sel pharmaceutiquement acceptable selon la revendication 1, dans lequel
    R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former un dihydropyrrolinyle ou tétrahydropyridinyle qui est non substitué ou substitué par alkyle en C1 à C4, ou bien R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former pipéridinyle, qui est substitué par alkyle en C1 à C4 sur au moins une position de celui-ci ; et
    R3 est un hydrogène ; alkyle en C1 à C6 ; phényle ; ou alkyle en C1 à C4 substitué par phényle,
    où le phényle est non substitué ou substitué par au moins un substituant non-hydrogène choisi dans le groupe constitué par un halogène, alcoxy en C1 à C4, halogénoalkyle en C1 à C4, et halogénoalcoxy en C1 à C4.
     
    6. Composé de formule 1 ou son sel pharmaceutiquement acceptable selon la revendication 1, dans lequel
    R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former un dihydropyrrolinyle ou tétrahydropyridinyle qui est non substitué ou substitué par alkyle en C1 à C2, ou bien R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former pipéridinyle, qui est substitué par un ou deux groupes alkyle en C1 à C2 ; et
    R3 est un hydrogène ; alkyle en C3 à C6 ; phényle ; ou alkyle en C1 à C2 substitué par phényle,
    où le phényle est non substitué ou substitué par au moins un substituant non-hydrogène choisi dans le groupe constitué par un halogène, alcoxy en C1 à C2, halogénoalkyle en C1 à C2, et halogénoalcoxy en C1 à C2.
     
    7. Composé de formule 1 ou son sel pharmaceutiquement acceptable selon la revendication 1, dans lequel
    R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former un dihydropyrrolinyle ou tétrahydropyridinyle qui est non substitué ou substitué par méthyle, ou bien R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former une pipéridine ; et
    R3 est un hydrogène ; butyle ; propyle ; hexyle ; phényle ; ou méthyle substitué par phényle,
    où la pipéridine est substituée par un ou deux groupes méthyle sur au moins l'une des positions 2, 3, 5 et 6, et
    le phényle est non substitué ou substitué par au moins un substituant non-hydrogène choisi dans le groupe constitué par un halogène, méthoxy, trihalogénométhyle, et trihalogénométhoxy.
     
    8. Composé de formule 1 ou son sel pharmaceutiquement acceptable selon la revendication 1, lequel composé de formule 1 est l'un des suivants :

    N1-1,2-dihydropyrrole-N5-(4-trifluorométhoxy)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(4-trifluorométhyl)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(3-trifluorométhyl)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(4-fluoro)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(4-chloro)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(4-bromo)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(3-chloro,4-trifluorométhoxy)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(3-chloro,4-trifluorométhoxy)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(3-trifluorométhyl)benzylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(4-trifluorométhyl)benzylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(3-trifluorométhoxy)benzylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(4-trifluorométhoxy)phénylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(4-trifluorométhyl)phénylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(4-chloro)phénylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(4-chloro,3-trifluorométhyl)phénylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(3-trifluorométhyl)phénylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(4-fluoro)phénylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(4-bromo)phénylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(4-méthoxy)phénylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(3,4-diméthoxy)phénylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(4-trifluorométhoxy)benzylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(3-trifluorométhoxy)phénylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(4-trifluorométhyl)benzylbiguanide ;

    N1-(3-méthyl)-1,2-dihydropyrrole-N5-(4-chloro,3-trifluorométhyl)benzylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-trifluorométhoxy)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-trifluorométhyl)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(3-trifluorométhoxy)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(3-trifluorométhyl)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-fluoro,3-trifluorométhyl)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-chloro,3-trifluorométhoxy)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(3-fluoro,4-trifluorométhoxy)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-chloro)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-bromo)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-fluoro)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(3,5-diméthoxy)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-méthoxy)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(3-méthoxy)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(4-méthoxy)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(3-méthoxy)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(3,5-diméthoxy)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(4-fluoro,3-trifluorométhyl)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(3-fluoro,4-trifluorométhyl)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-méthyl)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(3-méthyl)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(4-méthyl)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(3-méthyl)phénylbiguanide ;

    N1-1,2-dihydropyrrole-N5-(3-trifluorométhoxy)phénylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-hexylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-trifluorométhoxy)benzylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(3-trifluorométhoxy)benzylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-trifluorométhyl)benzylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(3-trifluorométhyl)benzylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-(4-chloro,3-trifluorométhyl)benzylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-butylbiguanide ;

    N1-1,2,3,6-tétrahydropyridine-N5-propylbiguanide ;

    N1-1,2,3,6-tétrahydropyridinebiguanide ;

    N1-(3-méthyl)pipéridine-N5-(3-trifluorométhyl)benzylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-chloro)benzylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-fluoro)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-bromo)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-chloro,3-trifluorométhyl)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(3-fluoro,4-trifluorométhyl)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-fluoro,3-trifluorométhyl)phénylbiguanide ;

    N1-(2-méthyl)pipéridine-N5-(4-trifluorométhoxy)phénylbiguanide ;

    N1-(2-méthyl)pipéridine-N5-(3-trifluorométhoxy)phénylbiguanide ;

    N1-(2-méthyl)pipéridine-N5-(4-trifluorométhyl)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(3-fluoro,4-trifluorométhoxy)phénylbiguanide ;

    N1-(2-méthyl)pipéridine-N5-(3-fluoro,4-trifluorométhoxy)phénylbiguanide ;

    N1-(2-méthyl)pipéridine-N5-(4-chloro)phénylbiguanide ;

    N1-(2-méthyl)pipéridine-N5-(4-fluoro,3-trifluorométhyl)phénylbiguanide ;

    N1-(2-méthyl)pipéridine-N5-(3-trifluorométhyl)phénylbiguanide ;

    N1-(2-méthyl)pipéridine-N5-(4-chloro,3-trifluorométhyl)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-trifluorométhyl)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-trifluorométhoxy)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(3-trifluorométhoxy)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-trifluorométhoxy)benzylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-fluoro,3-trifluorométhyl)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-trifluorométhyl)benzylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(4-chloro)phénylbiguanide ;

    N1-(3-méthyl)pipéridine-N5-(3-trifluorométhyl)phénylbiguanide ;

    N1-(2,6-diméthyl)pipéridine-N5-(4-trifluorométhoxy)phénylbiguanide ;

    N1-(2,6-diméthyl)pipéridine-N5-(3-trifluorométhoxy)phénylbiguanide ;

    N1-(2,6-diméthyl)pipéridine-N5-(4-trifluorométhyl)phénylbiguanide ;

    N1-(2,6-diméthyl)pipéridine-N5-(3-trifluorométhyl)phénylbiguanide ;

    N1-(2,6-diméthyl)pipéridine-N5-(4-fluoro,3-trifluorométhyl)phénylbiguanide ;

    N1-(2,6-diméthyl)pipéridine-N5-(4-chloro,3-trifluorométhyl)phénylbiguanide ;

    N1-(2,6-diméthyl)pipéridine-N5-(3-fluoro,4-trifluorométhoxy)phénylbiguanide ;

    N1-(2,6-diméthyl)pipéridine-N5-(4-chloro)phénylbiguanide ;

    N1-(2,6-diméthyl)pipéridine-N5-(4-bromo)phénylbiguanide ; et

    N1-(2,6-diméthyl)-N5-(4-fluoro)phénylbiguanide.


     
    9. Composé de formule 1 ou son sel pharmaceutiquement acceptable selon la revendication 1,
    dans lequel le sel pharmaceutiquement acceptable est un sel avec un acide choisi dans le groupe constitué par l'acide formique, l'acide acétique, l'acide propionique, l'acide lactique, l'acide butyrique, l'acide isobutyrique, l'acide trifluoroacétique, l'acide malique, l'acide maléique, l'acide malonique, l'acide fumarique, l'acide succinique, le monoamide d'acide succinique, l'acide glutamique, l'acide tartrique, l'acide oxalique, l'acide citrique, l'acide glycolique, l'acide glucuronique, l'acide ascorbique, l'acide benzoïque, l'acide phtalique, l'acide salicylique, l'acide anthranilique, l'acide benzènesulfonique, l'acide p-toluènesulfonique, l'acide méthanesulfonique, l'acide dichloroacétique, l'acide aminoxyacétique, l'acide chlorhydrique, l'acide bromhydrique, l'acide sulfurique, l'acide phosphorique, l'acide nitrique, l'acide carbonique, et l'acide borique.
     
    10. Procédé de préparation d'un composé de formule 1 qui suit, comprenant :

    la réaction d'un composé de formule 2 qui suit avec un dicyanamide dans un solvant organique pour que soit obtenu un composé de formule 3 qui suit ; et

    la réaction du composé de formule 3 qui suit avec un composé de formule 4 dans un solvant organique pour que soit obtenu le composé de formule 1 qui suit :







    formules dans lesquelles R1 et R2 sont pris conjointement avec l'azote auquel ils sont rattachés pour former un hétérocycloalcène en C3 à C6 ayant 4 à 7 atomes de cycle ou pipéridinyle, qui est substitué par alkyle en C1 à C4 sur au moins une position de celui-ci ; et

    R3 est un hydrogène ; alkyle en C1 à C6 ; phényle ; ou alkyle en C1 à C4 substitué par phényle,

    où l'hétérocycloalcène en C3 à C6 est non substitué ou substitué par au moins un substituant choisi dans le groupe constitué par un halogène, hydroxy et alkyle en C1 à C6,

    le phényle est non substitué ou substitué par au moins un substituant non-hydrogène choisi dans le groupe constitué par un halogène, hydroxy, alkyle en C1 à C6, alcoxy en C1 à C6, halogénoalkyle en C1 à C6, et halogénoalcoxy en C1 à C6.


     
    11. Composition pharmaceutique pour une utilisation dans la prévention ou le traitement d'un cancer, comprenant, en tant que principe actif, le composé de formule 1 ou son sel pharmaceutiquement acceptable défini dans l'une quelconque des revendications 1 à 9.
     
    12. Composition pharmaceutique pour une utilisation selon la revendication 11, dans laquelle le traitement du cancer comprend une inhibition de la récurrence ou de la métastase du cancer.
     
    13. Composition pharmaceutique pour une utilisation selon la revendication 11, dans laquelle le cancer est une maladie choisie dans le groupe constitué par un cancer utérin, un cancer du sein, un cancer gastrique, un cancer du cerveau, un cancer rectal, un cancer colorectal, un cancer du poumon, un cancer de la peau, un cancer du sang, un cancer pancréatique, un cancer de la prostate, et un cancer du foie.
     
    14. Composition pharmaceutique pour une utilisation selon la revendication 11, laquelle composition pharmaceutique est formulée sous la forme d'un comprimé, d'une capsule, d'une pilule, d'un granule, d'une poudre, d'une composition injectable ou d'un liquide.
     






    Cited references

    REFERENCES CITED IN THE DESCRIPTION



    This list of references cited by the applicant is for the reader's convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard.

    Patent documents cited in the description




    Non-patent literature cited in the description