(19)
(11)EP 3 135 762 B1

(12)EUROPEAN PATENT SPECIFICATION

(45)Mention of the grant of the patent:
10.07.2019 Bulletin 2019/28

(21)Application number: 15800046.3

(22)Date of filing:  22.05.2015
(51)International Patent Classification (IPC): 
C12N 9/90(2006.01)
C12N 15/61(2006.01)
(86)International application number:
PCT/KR2015/005208
(87)International publication number:
WO 2015/182937 (03.12.2015 Gazette  2015/48)

(54)

PSICOSE EPIMERASE AND PSICOSE PRODUCTION METHOD USING SAME

PSICOSE-EPIMERASE UND PSICOSEHERSTELLUNGSVERFAHREN DAMIT

PSICOSE ÉPIMÉRASE ET PROCÉDÉ DE PRODUCTION DU PSICOSE L'UTILISANT


(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30)Priority: 28.05.2014 KR 20140064651

(43)Date of publication of application:
01.03.2017 Bulletin 2017/09

(73)Proprietor: Daesang Corporation
Seoul 130-706 (KR)

(72)Inventors:
  • KIM, Tae Gyun
    Seoul 140-133 (KR)
  • KIM, Min Su
    Seoul 131-814 (KR)
  • KIM, Tae Yong
    Seoul 143-853 (KR)
  • SONG, Eun Bum
    Seoul 137-911 (KR)
  • OH, Deok Kun
    Gwacheon-si Gyeonggi-do 427-731 (KR)

(74)Representative: Ter Meer Steinmeister & Partner 
Patentanwälte mbB Nymphenburger Straße 4
80335 München
80335 München (DE)


(56)References cited: : 
WO-A1-2006/129954
WO-A2-2011/040708
KR-A- 20140 021 974
WO-A1-2014/049373
KR-A- 20110 035 805
  
  • WANMENG MU ET AL: "Recent advances on applications and biotechnological production of-psicose", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 94, no. 6, 10 May 2012 (2012-05-10), pages 1461-1467, XP035060669, ISSN: 1432-0614, DOI: 10.1007/S00253-012-4093-1
  • DATABASE NCBI [Online] 09 May 2013 ncbi: 'AP endonuclease [Clostridiales', XP055240988 Database accession no. WP_007494289
  
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).


Description

[Technical Field]



[0001] The present invention relates to a novel psicose epimerase capable of converting fructose into D-psicose, a method of producing the psicose epimerase from a recombinant strain, a method of producing D-psicose from fructose using the same, and the like.

[Background Art]



[0002] D-psicose as an epimer of a carbon-3 position of fructose has 70% sweetness as compared with sugar (Oshima 2006), but is functional monosaccharide applicable as a low-calorie sweetener of diet food in which energy is only 0.3% (Matsuo et al. 2002). Further, the D-psicose serves to inhibit glucose by suppressing absorption of glucose to be applied to food for diabetic patients, receiving food, and the like and serves to suppress the enzyme activity associated with lipid synthesis in the liver to suppress the accumulation of abdominal fat to be used in various functional foods such as health foods (Matsuo et al. 2001; Iida et al. 2008; Hayashi et al. 2010; Hossain et al. 2011).

[0003] As the above features, the psicose is a good source capable of replacing sugar, but the psicose belongs to a rare saccaride as a monosaccaride that is rarely present in the nature, and thus, a method for efficiently producing the psicose is required so as to be applied to food industry. The psicose is mostly produced through a chemical process as an existing method of producing the psicose. Bilik, et al. propose a method of converting fructose into psicose by using a catalytic action of molybdate ions. McDonald produced the psicose by a 3-step chemical treatment process from 1,2:4,5-di-δ-isopropylidene-beta-D-fructopyranose. Further, Doner produced the psicose by heating the fructose together with ethanol and trimethylamine. However, there are disadvantages in that a lot of costs are consumed in the chemical production methods, whereas the efficiencies thereof are low and a lot of byproducts are generated.

[0004] As a biological production method of psicose, a method of producing psicose from galactitol, D-tagatose, D-talitol, or the like by using a cell reaction of a microorganism is proposed (Ken Izumori). However, the method is difficult to be applied to the industrial production because the substrate is the rare saccaride. The most efficient method for industrialization is a method of finding an enzyme for converting fructose into psicose in a D-ketose 3-epimerase group. By the existing reported contents, the psicose was produced from the fructose by using a D-tagatose 3-epimerase which is expressed in E. coli transformed by inserting and transforming the D-tagatose 3-epimerase derived from Clostridium celluloticum H (10) (Mu et al. 2011), Agrobacterium tumefaciens (Kim et al. 2006), Pseudomonas cichorii (Itoh et al. 1994), Rhizobium spheroides (Zhang et al. 2009) in E. coli. With relation to a technique of producing psicose from fructose by using an enzyme, in Korea Patent Registration No. 10-0744479, a method of producing psicose by a psicose epimerase derived from Agrobacterium tumefaciens is disclosed, in Korea Patent Registration No. 10-0832339, Sinorhizobium YB-58 KCTC 10983BP having an activity of converting fructose into psicose and a method for converting fructose into psicose by using the same are disclosed, in Korea Patent Registration No. 10-1106253, E. coli including a polynucleotide coding a psicose 3-epimerase of Agrobacterium tumefaciens C58 having an activity of catalyzing conversion of fructose into psicose and a method of producing psicose from fructose by using the same are disclosed, in Korea Patent Registration No. 10-1339443 (Korea Patent Application Publication No. 10-2008-0071176), a ketose 3-epimerase derived from microorganisms belonging to genus Rhizobium and a method for converting fructose into psicose by using the same are disclosed, and in Korea Patent Registration No. 10-1318422, a D-psicose 3-epimerase derived from Clostridiuim scindens and a method for producing psicose from fructose by using the same are disclosed. WO 2014/049373 A1 relates to a protein which has ketose-3-epimerase activity and which comprises a polypeptide sequence having at least 70% sequence identity to SEQ ID NO:6, SEQ ID NO:2 or SEQ ID NO:4. WO 2006/129954 A1 relates to a method of producing D-psicose using a D-psicose epimerase derived from Agrobacterium tumefaciens. Wanmeng Mu et al., Applied Microbiology and Biotechnology, Springer, Berlin, DE, vol.94, no.6, 10 May 2012, pp. 1461-1467is a review about the production and use of D-psicose. WO 2011/040708 A2 relates to a method of successively producing D-psicose from D-fructose or D-glucose by using a psicose-epimerase derived from Agrobacterium tumefaciens which is expressed in a food safety form. NCBI database entry WP_007494289.1, "AP endonuclease Clostridiales" is a database entry relating to a sequence from Clostridiales.

[0005] However, according to an existing enzymatic method of which functions are known, a method of producing psicose is best at a medium temperature and pH of an alkaline condition. In the alkaline condition, the reaction induces non-specific reaction and browning of sugar, and thus is not suitable for the industrialization. Further, there is a problem in that the existing enzymes have factors of increasing producing costs for producing psicose which is applied to the industrialization due to deteriorated stability or a slow reaction rate at a high temperature. Therefore, development of a novel D-psicose 3-epimerase in which a product yield, a temperature, a pH and a reaction rate of the psicose are suitable for industrialization is required. With relation to this, in Korea Patent Application Publication No. 10-2014-0021974, a D-psicose 3-epimerase derived from Treponema primitia ZAS-1 having a rapid psicose conversion rate and stability at a high temperature by inducing mutation at a gene level is disclosed, and in Korea Patent Registration No. 10-1203856, psicose epimerase variants having improved thermal stability obtained by mutation of a wild-type psicose epimerase derived from Agrobacterium tumefaciens is disclosed.

[Disclosure]


[Technical Problem]



[0006] A first object of the present invention is to provide a novel D-psicose 3-epimerase having an activity of converting fructose into psicose, a maximum activity at a relatively high temperature or a pH less than or equal to neutral, and excellent thermal stability.

[0007] A second object of the present invention is to provide a method of producing a novel D-psicose 3-epimerase or various elements required for producing the novel D-psicose 3-epimerase.

[0008] A third object of the present invention is to provide a method of producing psicose from fructose or various elements required for producing the psicose from the fructose.

[Technical Solution]



[0009] The inventors of the present invention discovered that an enzyme derived from Flavonifractor plautii has a high activity for converting fructose into psicose, excellent thermal stability, and an optimal activity in a high temperature range and a pH range corresponding to neutral or weak acid, and thus, completed the present invention.

[0010] An aspect of the present disclosure provides a psicose epimerase consisting of an amino acid sequence of SEQ ID NO: 1.

[0011] Another aspect of the present disclosure provides a polynucleotide coding a psicose epimerase consisting of an amino acid sequence of SEQ ID NO: 1. Further, the present disclosure provides a primer pair for synthesizing the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1. Further, the present disclosure provides a recombinant vector including the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1. Further, the present disclosure provides a recombinant strain which is transformed by the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or the recombinant vector including the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1. Further, the present disclosure provides a method for producing psicose epimerase, in which the method includes steps of: expressing the psicose epimerase by culturing a recombinant strain which is transformed by a polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or a recombinant vector including the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1; and isolating the psicose epimerase from a lysate of the recombinant strain with the expressed psicose epimerase.

[0012] In order to solve the third object, yet another aspect of the present disclosure provides a composition for producing psicose including a psicose epimerase consisting of an amino acid sequence of SEQ ID NO: 1. Further, the present disclosure provides the composition for producing psicose including a recombinant strain which is transformed by a polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or a recombinant vector including the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1, a culture of the recombinant strain, or a lysate of the recombinant strain. Further, the present invention provides the method for producing psicose, in which the method includes reacting fructose with the psicose epimerase consisting of an amino acid sequence of SEQ ID NO: 1 or the composition including the psicose epimerase. Further, the present invention provides the method for producing psicose according to claim 1.

[Advantageous Effects]



[0013] The novel psicose epimerase, according to the present disclosure, possesses an activity producing psicose by epimerizing the carbon-3 position of fructose, and has maximal activity for the conversion of fructose into psicose at a relatively high temperature and a pH less than or equal to neutral, has excellent thermal stability, and can mass-produce psicose from fructose in a high yield for a short amount of time. Therefore, the psicose epimerase according to the present diclosure is advantageous in the industrial production of psicose, and the psicose produced thereby can be usefully utilized in the functional sugar industry and as materials for health food, medicine, cosmetics, and the like using the psicose.

[Description of Drawings]



[0014] 

FIG. 1 is a cleavage map of pET-FDPE which is a recombinant expression vector.

FIG. 2 is a result of performing SDS-PAGE with respect to a D-psicose 3-epimerase purified through a His tag affinity chromatography in Example 2 of the present disclosure.

FIG. 3 is a graph illustrating an activity of the D-psicose 3-epimerase of the present disclosure according to a kind of added metal ion. In FIG. 3, an enzyme activity in the case of treating each metal ion is relatively represented by setting an enzyme activity in a control group to 100.

FIG. 4 is a graph illustrating an activity of the D-psicose 3-epimerase of the present disclosure for each kind of added metal ion and each treated concentration.

FIG. 5 is a graph illustrating an activity of the D-psicose 3-epimerase of the present disclosure for each reaction pH. In FIG. 5, the enzyme activity is relatively represented by setting a maximum activity to 100.

FIG. 6 is a graph illustrating an activity of the D-psicose 3-epimerase of the present disclosure for each reaction temperature. In FIG. 6, the enzyme activity is relatively represented by setting a maximum activity to 100.

FIG. 7 is a graph illustrating an activity of the D-psicose 3-epimerase of the present disclosure for each substrate. In FIG. 7, the enzyme activity is relatively represented by setting an enzyme activity of a reaction using psicose as a substrate to 100.


[Detailed Description of Embodiment]



[0015] Hereinafter, the present invention will be described in detail.

[0016] The present disclosure relates to a novel D-psicose 3-epimerase (hereinafter, referred to as a psicose epimerase) capable of converting fructose into psicose. The psicose epimerase is derived from Flavonifractor plautii, and has maximal activity for the conversion of the fructose into the psicose at a relatively high temperature and a pH less than or equal to neutral, has excellent thermal stability, and can mass-produce the psicose from the fructose in a high yield for a short amount of time. The psicose epimerase may be obtained by a method of amplifying a specific DNA in many DNAs present in a Flavonifractor plautii genomic DNA by a polymerase reaction, preparing a recombinant expression vector by inserting the amplified specific DNA to an expression vector, producing a recombinant strain by transforming a host strain with the recombinant expression vector, and then culturing and expressing the recombinant strain. The psicose epimerase according to the present disclosure preferably has a molecular weight of 30 to 34 kDa, an optimal activity temperature in a range of 55 to 67°C, and an optimal activity pH in a range of 6.5 to 8. The psicose epimerase according to the present disclosure consists of an amino acid sequence of SEQ ID NO: 1, but an equivalent range of the psicose epimerase according to the present invention is not limited thereto. For example, in the equivalent range of the psicose epimerase according to the present disclosure, so long as the activity of converting the fructose into the psicose is maintained, some of amino acids of SEQ ID NO: 1 may be replaced, inserted, and/or deleted. Preferably, the replacement of the amino acid may be performed by conservative amino acid replacement in which a characteristic of a protein is not changed. Further, the modification of the amino acid may be performed by glycosylation, acetylation, phosphorylation, and the like. Further, the equivalent range of the psicose epimerase according to the present diclosure may include a protein with increased structural stability to heat, pH, and the like by mutation or modification of the amino acid sequence or increased activity for the conversion of fructose into psicose. Further, the equivalent range of the psicose epimerase according to the present disclosure may include amino acid sequences having homology of 70% or more, 80% or more, 90% or more, 95% or more, or 99% or more with the amino acid sequence of SEQ ID NO: 1. The following Table 1 lists amino acids which may replace amino acids in the protein by the conservative amino acid replacement.
[Table 1]
Amino acid residues in peptideConservative replacement group
Ala Ser
Arg Lys
Asn Gln, His
Asp Glu
Gln Asn
Cys Ser
Glu Asp
Gly Pro
His Asn, Gln
Ile Leu, Val
Leu Ile, Val
Lys Arg, Gln
Met Leu, Ile
Phe Met, Leu, Tyr
Ser Thr, Gly
Thr Ser, Val
Trp Tyr
Tyr Trp, Phe
Val Ile, Leu


[0017] Another exemplary embodiment of the present disclosure relates to a method of producing a novel psicose epimerase or various elements required for producing the novel psicose epimerase. The various elements required for producing the novel psicose epimerase include polynucleotide, a primer pair, a recombinant vector, a recombinant strain, and the like.

[0018] The polynucleotide is polynucleotide coding an epimerase consisting of an amino acid sequence of SEQ ID NO: 1 and preferably consists of a base sequence of SEQ ID NO: 2. In the present disclosure, the term "polynucleotide" means all of non-modified or modified polyribonucleotides (RNA) or polydeoxyribonucleotides (DNA). The polynucleotide includes single- or double-stranded DNA, DNA which is a mixture of single- and double-stranded regions, single- and double-stranded RNA, RNA which is a mixture of single- and double-stranded regions, or hybrid molecules thereof, but is not limited thereto. Further, the equivalent range of the polynucleotide coding the epimerase includes a sequence having the substantial identity with a base sequence of SEQ ID NO: 2. The substantial identity means that any other sequence is arranged to maximally correspond to the base sequence of SEQ ID NO: 2 and the any other sequence has the sequence homology of 70% or more, 90% or more, or 98% or more with the base sequence of SEQ ID NO: 2 by analyzing the sequence thereof. It can be easily understood to those skilled in the art that one or more bases of the base sequence of the polynucleotide are replaced, added, or deleted by using a gene recombination technique known in the art to produce polynucleotide coding an enzyme having the same activity in a range having the substantial homology. A comparison of the homologies may be performed by calculating homology between two or more sequences in percentage (%) by using a computer program on the market.

[0019] Further, the primer pair is to synthesize polynucleotide coding a psicose epimerase consisting of an amino acid sequence of SEQ ID NO: 1, and preferably constituted by a forward primer having a base sequence of SEQ ID NO: 3 and a reverse primer having a base sequence of SEQ ID NO: 4.

[0020] Further, the recombinant vector includes polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1. The recombinant vector may be provided in a form of inserting the polynucleotide coding the psicose epimerase into a cloning vector or an expression vector by using a known standard method. In the present disclosure, the term "cloning vector" is defined as a material which can transport a DNA fragment into a host cell and reproduce the DNA fragment. In the present disclosure, the cloning vector may further include a polyadenylation signal, a transcription termination sequence, and a multiple cloning site. In this case, the multiple cloning sites include at least one endonuclease restriction site. Further, the cloning vector may further include a promoter. As an example, in the present disclosure, the polynucleotide coding the psicose epimerase may be located at the upstream of the polyadenylation signal and the transcription termination sequence, and at least one endonuclease restriction site may be located at the upstream of the polyadenylation signal and the transcription termination sequence. Further, in the present disclosure, the term "expression vector" is defined as a DNA sequence required for transcription and translation of the cloned DNA in an appropriate host. Further, in the present disclosure, the term "expression vector" means a gene construct including a required regulation element which is operably connected to an insertion so as to express the insertion in cells of an object. The expression vector may be produced and purified by using a standard recombinant DNA technique. A kind of expression vector is not particularly limited as long as the expression vector expresses a desired gene in various host cells, such as, prokaryotic and eukaryotic cells and performs a function of producing a desired protein, but is preferably a vector which may mass-produce a foreign protein in a similar form to a natural state, while possessing a promoter representing a strong activity and strong expression force. The expression vector preferably includes at least a promoter, an initiation codon, a gene coding a desired protein, and a stop codon terminator. In addition, the expression vector may appropriately include a DNA coding a signal peptide, an additional expression regulating sequence, non-translation regions of 5'-terminal and 3'-terminal of a desired gene, a selection marker region, a replicable unit, or the like. The "promoter" means a minimal sequence enough to indicate the transcription. Further, a promoter configuration enough to express a regulatory promoter-dependent gene induced by a cell type-specific or external signal or agent may be included, and the configurations may be located at the 5' or 3' portion of the gene. Both a conservative promoter and an induction promoter are included. The promoter sequence may be derived from prokaryote, eukaryote, or virus. The term "operably connected" means that one function is regulated by another thing by association with a polynucleotide sequence on a single polynucleotide. For example, in the case where the promoter may control expression of the coding sequence (that is, the coding sequence is under the transcription regulation of the promoter), if the promoter is connected with the coding sequence to operate or a ribosome binding site is located to promote the translation, the ribosome binding site is connected to the coding sequence and operates. The coding sequence may be connected to a regulation sequence and operate in a sense direction or an antisense direction. In the expression vector according to the present disclosure, the recombinant vector is preferably an expression vector and the expression vector preferably has a cleavage map of FIG. 1.

[0021] Further, the recombinant strain is transformed by the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or transformed by a recombinant vector including the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1. In the present disclosure, the term "recombinant strain" means cells transformed by introducing a polynucleotide coding one or more target proteins or an expression vector having the polynucleotide to a host cell. A method for producing a transformant by introducing the expression vector to the host cell includes a chemical treating method including transient transfection, microinjection, transduction, cell fusion, calcium phosphate precipitation, liposemmediated transfection, DEAE Dextran-mediated transfection, polybrene-mediated transfection, electroporation, electroinjection, PEG, and the like, a method using a gene gun and the like, and the like, but is not limited thereto. In the present disclosure, as long as a host cell which may be transformed by the expression vector is known in the art, such as prokaryotic cells, plant cells, insect cells, animal cells, and the like, a kind thereof is largely not limited thereto. Preferably, a host having high introduction efficiency of DNA and high expression efficiency of the introduced DNA is generally used. For example, the host cell may be E. coli. The E. coli includes BL21, JM109, K-12, LE392, RR1, DH5α, W3110, or the like, but is not limited thereto. In addition, the host cell may be a strain selected from the group consisting of Bacillus strains such as Bacillus subtilis and Bacillus thuringiensis, Coryne bacterial strains such as Corynebacterium glutamicum, Salmonella strains such as Salmonella typhimurium, other Serratia marcescens, and Enterobacteriaceae strains such as various Pseudomonas species.

[0022] Further, a method of producing the psicose epimerase includes the steps of expressing psicose epimerase by culturing a recombinant strain which is transformed by a polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or a recombinant vector including the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1; and isolating the psicose epimerase from a lysate of the recombinant strain in which the psicose epimerase is expressed. The expression of the protein by the host cell may be induced by using isopropyl-1-thio-β-D-galactopyranoside (IPTG) as an inducing factor and an inducing time may be adjusted to maximize the amount of the protein. In the present disclosure, the psicose epimerase may be collected from a lysate of the recombinant strain. The cells used in protein expression may be lysed by various physical or chemical means such as freeze-thawing repetition, sonication, mechanical breakage, or a cell lytic agent and can be isolated or purified by a general biochemical isolation technique (Sambrook et al., Molecular Cloning: A laborarory Manual, 2nd Ed., Cold Spring Harbor Laboratory Press, 1989; Deuscher, M., Guide to Protein Purification Methods Enzymology, Vol. 182. Academic Press. Inc., San Diego, CA, 1990). For example, the method of isolating or purifying the protein expressed by the host cell includes electrophoresis, centrifugation, gel filtration, precipitation, dialysis, chromatography (ion exchange chromatography, affinity chromatography, immunosorbent affinity chromatography, reverse-phase HPLC, and gel permeation HPLC), isoelectricity focus, and various modified or complex methods thereof, but is not limited thereto. Meanwhile, in the present disclosure, the isolating of the psicose epimerase from the lysate of the recombinant strain may be preferably performed by affinity chromatography using a peptide tag. As the peptide tag, various known tags such as a HA tag, a FLAG tag, a His tag, a biotin carboxyl carrier protein (BCCP), a c-myc tag, a V5 tag, a glutathione-S-transferase (GST), or a maltose binding protein (MBP) may be used and among them, the His tag is preferably used. The His-tagging protein is specifically trapped on a column of a nickel-nitrilotriacetic acid (Ni-NTA) resin and may be released by EDTA or imidazole.

[0023] Yet another exemplary embodiment of the present invention relates to a method of producing psicose from fructose or various elements required for producing the psicose from the fructose. As various elements required for producing the psicose from the fructose, there is a composition for producing the psicose.

[0024] An example of the composition for producing the psicose includes a psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1. Further, another example of the composition for producing the psicose includes a recombinant strain which is transformed by the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or a recombinant vector including the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1, a culture of the recombinant strain, or a lysate of the recombinant strain. In this case, preferably, the composition for producing the psicose may further include one or more kinds selected from the group consisting of manganese ions, nickel ions, and cobalt ions, and more preferably, may further include nickel ions or cobalt ions. The novel psicose epimerase according to the present invention has a metalloenzyme characteristic in which activation is adjusted by a metal ion and performs the reaction by the enzyme in a presence of a specific metal ion such as nickel ions or cobalt ions to increase a production yield of the psicose.

[0025] Further, an example of the method of producing the psicose from the fructose includes reacting the fructose with the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or the composition including the psicose epimerase. Further, another example of the method of producing the psicose from the fructose includes reacting the fructose with a recombinant strain which is transformed by a polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or a recombinant vector including the polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1, a culture of the recombinant strain, a lysate of the recombinant strain, or a composition including one or more thereof. Further, the method of producing the psicose from the fructose may additionally include adding metal ions, and a kind of metal ion is as described above. As an example, the metal ion may be added to the fructose that is a substrate or added to a mixture of the enzyme and the fructose. Further, as another example, the metal ion may be added to a carrier immobilized with the enzyme (before adding the fructose), added to a mixture of the carrier immobilized with the enzyme and the fructose (after adding the fructose), or added in a form of the mixture with the fructose when the fructose is added. In the case of using the recombinant strain, the metal ion may be added in the culture or the culturing may be performed in a culture medium added with the metal ion. Further, in the method of producing the psicose from the fructose, the psicose epimerase or the recombinant strain is preferably immobilized in the carrier. The carrier may create an environment in which the activity of the immobilized enzyme may be maintained for a long amount of time, and may be selected from all known carriers which may be used for enzyme immobilization. For example, sodium alginate may be used as the carrier. The sodium alginate is a natural colloidal polysaccharide abundant in the cell wells of algae and consists of β-D-mannuronic acid and α-L-gluronic acid. In terms of the content thereof, the sodium alginate is formed by randomly forming a β-1,4 bond and the strain or the enzyme is stably immobilized, and thus it is advantageous to have excellent psicose yield. As an example, in order to further promote the yield of the psicose, a sodium alginate solution at a concentration of 1.5 to 4.0% (w/v) (for example, an aqueous sodium alginate solution), preferably a sodium alginate solution at a concentration of about 2.5% (w / v) may be used for immobilizing the recombinant strain. Further, in the method of producing the psicose from the fructose, the reaction temperature is in the range of 55 to 67°C, preferably 55 to 65°C, and more preferably 55 to 60°C when considering stability of the enzyme, and the reaction pH is in the range of 6.5 to 8, preferably 6.5 to 7.5, and more preferably 6.5 to 7. Further, in the method of producing the psicose from the fructose, the concentration of the fructose is not particularly limited thereto, but preferably 35 to 75% (w/w) and more preferably 40 to 70% (w/w) based on the entire reactant when considering productivity and economics. Further, in the method of producing the psicose from the fructose, the amount of used enzyme may be 0.001 to 0.1 mg/ml, preferably 0.01 to 0.1 mg/ml, and more preferably 0.02 to 0.05 mg/ml based on the entire reactant. Further, in the case of producing the psicose from the fructose by using the recombinant strain, the host strain of the recombinant strain is preferably a cytologically safe strain. The cytologically safe strain means a generally accepted as safe (GRAS) strain which is generally accepted as safe and for example, may be a corynebacterium strain. The corynebacterium strain is an industrial microorganism which produces chemical materials having various uses in fields of feed, medicines, and food, and the like including L-lysine, L-threonine, and various nucleic acids. The corynebacterium strain is the GRAS strain and has a strain characteristic which is easily used for gene manipulation and mass culture. Further, the corynebacterium strain is a strain having high stability in various process conditions and has a relatively hard cell membrane structure as compared with other bacteria to have a biological characteristic in which the strain is present in a stable state even under high osmotic pressure caused by high sugar concentration. A particular example of the corynebacterium strain includes Corynebacterium glutamicum and the like.

[0026] Hereinafter, the present invention will be described in more detail through Examples. However, the following Examples are just for clearly exemplifying the technical features of the present invention, and the scope of the present invention is not limited thereto.

Example 1: Preparation of recombinant strain producing D-psicose 3-epimerase



[0027] A genomic DNA was extracted from Flavonifractor plautii KCTC 5970 received from Korean Collection for Type Cultures and used as a template, and a polymerase chain reaction (PCR) was performed by using a primer and an Ex-Taq (TAKARA) polymerase for cloning a gene (a polynucleotide of SEQ ID NO: 2) coding D-psicose 3-epimerase. The following Table 2 lists a primer used for cloning the gene coding the D-psicose 3-epimerase from the genomic DNA of Flavonifractor plautii. The primers listed in the following Table 2 were prepared by Bioneer co., KR.
[Table 2]
SEQ ID NOPrimer typeBase sequence (5'→3')Restriction enzyme recognition site included in primer
3 Forward primer for cloning psicose epimerase

 
Nde I
4 Reverse primer for cloning psicose epimerase

 
Xho I


[0028] Thereafter, only a desired target DNA was isolated from a PCR product by using a gel extraction kit (Qiagen) and then bound to an easy T-vector (Promega). A base sequence analysis of the isolated target DNA was entrusted to Bioneer Co., KR. As a result, it was verified that the target DNA amplified through the PCR corresponded to the polynucleotide of SEQ ID NO: 2. Thereafter, pET-FDPE as a recombinant expression vector was prepared by inserting the target DNA amplified by the PCR reaction to the same restriction recognition site of a pET15b vector (Novagen) as the expression vector by using Nde I and Xho I which were restrictive enzymes. FIG. 1 is a cleavage map of pET-FDPE which is a recombinant expression vector. Thereafter, a recombinant strain was prepared by transforming BL21 (manufacturer: RBC, Taipei, Taiwan) E. coli as a competent cell to the pET-FDPE as the recombinant expression vector by using electroporation.

Example 2: Expression and purification of D-psicose 3-epimerase



[0029] A single colony of the transformed recombinant strain was inoculated in 15 ml of a LB-ampicillin medium (Difco) and then pre-cultured for about 6 hrs in a condition of 37°C and 200 rpm. Thereafter, the pre-culture solution was inoculated in 500 ml of a LB-ampicilline medium and shaking-cultured in a condition of 37°C and 200 rpm. Thereafter, when the absorbance (at 600 nm) of the culture solution was 0.5, IPTG was added to be a concentration of 0.1 mM to induce overexpression of a target enzyme. In this case, the culture from the overexpression inducing time was converted to a condition of 16°C and 150 rpm and maintained for about 16 hrs. Thereafter, the culture solution of the recombinant strain was centrifuged for 2 mins at 13,000 rpm and a supernatant was removed to collect strain cells of the recombinant strain.

[0030] The collected strain cells of the recombinant strain were suspended in a lysis buffer (50mM Tris_HCl 300 mM NaCl pH 8.0, 10 mM imidazole) and then lysed by ultrasonic treatment. The cell lysate was centrifuged for 10 mins at 13,000 rpm and only the supernatant was gathered and then applied to a Ni-NTA column (Bio-Rad, Profinia) pre-equilibrated with a lysis buffer, and buffer solutions including 20 mM imidazole and 200 mM imidazole were sequentially flowed in 50mM Tris_HCl 300 mM NaCl pH 8.0. Finally, 50mM Tris_HCl 300mM NaCl pH 8.0 and 200 mM imidazole were flowed to elute a target protein. It was verified from an experimental reult to be described below that eluted protein was the D-psicose 3-epimerase. Thereafter, the eluted protein was stored in a buffer solution (PIPES, pH 7.5) for measuring an enzyme activity so as to be used for a next experiment. Further, the SDS-PAGE for the eluted protein was performed, and thus, it was verified that the size of the eluted protein was 32 kDa. FIG. 2 is a result of performing SDS-PAGE for a D-psicose 3-epimerase purified through a His tag affinity chromatography in Example 2 of the present invention.

Example 3: Verification of characteristics of D-psicose 3-epimerase


(1) Analysis of metal ion requirement of D-psicose 3-epimerase



[0031] Whether the metal ion had an effect on the D-psicose 3-epimerase obtained in Example 2 was examined.

[0032] ZnSO4, CaCl2, MnSO4, NiSO4, CoCl2, MgSO4, CuSO4, CaCO3, and FeSO4 were put in the purified enzyme buffer solution (PIPES, pH 7.5) to be 1 mM, respectively and then the metal ions were bound to the enzymes for about 1 hr. Thereafter, the enzymes bound with the metal ions were mixed with a 100 mM aqueous fructose solution as a substrate at a weight ratio of 1:1 and the mixture in which the enzyme concentration was 0.025 ml/mg and the fructose concentration was 50 mM was made and reacted for 10 mins at 60°C. Then, a hydrochloric acid solution was added thereto to stop the reaction. Further, in a control group, the same experiment was performed by using an enzyme (None) without treating the metal ion. Thereafter, an amount (mM) of produced psicose was measured and divided into an enzyme amount and a reaction time to calculate an enzyme activity. The psicose amount was analyzed by HPLC. The HPLC analysis was performed while water 100% (v/v) as a mobile phase flowed at a flow velocity of 0.6 ml/min at 80°C by using a 87C (BIO-RAD) column and the psicose was detected with a refractive index detector (Agilent 1260 TID) to analyze the produced amount of psicose. FIG. 3 is a graph illustrating an activity of the D-psicose 3-epimerase of the present disclosure according to a kind of added metal ion. In FIG. 3, an enzyme activity in the case of treating each metal ion is relatively represented by setting an enzyme activity in a control group to 100. As illustrated in FIG. 3, in the D-psicose 3-epimerase obtained in Example 2, an activity of converting the fructose into the psicose was increased by adding manganese ions, nickel ions, and cobalt ions, and particularly, an increase in activity of the nickel ions and cobalt ions was significant.

[0033] Thereafter, MnSO4, NiSO4, and CoCl2 were treated at various concentrations in the purified D-psicose 3-epimerase solution and then the same experiments were performed. FIG. 4 is a graph illustrating an activity of the D-psicose 3-epimerase of the present invention for each kind of added metal ion and each treated concentration. As illustrated in FIG. 4, the manganese ions, the nickel ions, and the cobalt ions increased the activity of the D-psicose 3-epimerase in various concentration ranges.

(2) Analysis of activity of D-psicose 3-epimerase according to change in pH or temperature



[0034] In order to examine an optimal pH of the D-psicose 3-epimerase obtained in Example 2, test solutions of various pHs were made by using an MES buffer, a PIPES buffer, an EPPS buffer, and a CHES buffer. In detail, NiSO4 was treated at a concentration of 1 mM in a buffer solution of the purified enzyme and then mixed with a 100 mM aqueous fructose solution at a weight ratio of 1:1 to prepare the test solutions of various pHs having the buffer concentration of 50 mM, the enzyme concentration of 0.025 ml/mg, and the fructose concentration of 50 mM. Thereafter, the test solutions reacted for 10 mins at 60°C and a hydrochloric acid solution was added thereto to stop the reaction. Thereafter, the amount (mM) of produced psicose was analyzed and measured by the HPLC and divided into an enzyme amount and a reaction time to calculate an enzyme activity. FIG. 5 is a graph illustrating an activity of the D-psicose 3-epimerase of the present invention for each reaction pH. In FIG. 5, the enzyme activity is relatively represented by setting a maximum activity to 100. As illustrated in FIG. 5, the D-psicose 3-epimerase of the present invention had a high activity at pH of 6.5 to 8 and preferably pH of 6.5 to 7.5 and had a maximal activity at pH 7.

[0035] Further, NiSO4 was treated at a concentration of 1 mM in a buffer solution (PIPES, pH 7.5) of the purified enzyme and then mixed with a 100 mM aqueous fructose solution at a weight ratio of 1:1 to prepare a test solution having pH 7.0, the enzyme concentration of 0.025 ml/mg, and the fructose concentration of 50 mM. Thereafter, the test solution reacted for 10 mins at various temperatures and then a hydrochloric acid solution was added thereto to stop the reaction. Thereafter, the amount (mM) of produced psicose was analyzed and measured by the HPLC and divided into an enzyme amount and a reaction time to calculate an enzyme activity. FIG. 6 is a graph illustrating an activity of the D-psicose 3-epimerase of the present disclosure for each reaction temperature. In FIG. 6, the enzyme activity is relatively represented by setting a maximum activity to 100. As illustrated in FIG. 6, the D-psicose 3-epimerase of the present invention had a high activity at a temperature of 55 to 67°C and had a maximal activity at 65°C.

(3) Analysis of substrate specificity of D-psicose 3-epimerase



[0036] Reaction activities for various substrates such as psicose, fructose, and tagatose of the D-psicose 3-epimerase obtained in Example 2 were analyzed.

[0037] NiSO4 was treated at a concentration of 1 mM in a buffer solution (PIPES, pH 7.5) of the purified enzyme and then mixed with a 100 mM aqueous substrate solution at a weight ratio of 1:1 to prepare a test solution having pH 7.0, the enzyme concentration of 0.025 ml/mg, and the substrate concentration of 50 mM. Thereafter, the test solution reacted for 10 mins at 65°C and then a hydrochloric acid solution was added thereto to stop the reaction. Thereafter, an amount (mM) of epimer corresponding to the substrate was analyzed and measured by the HPLC and divided into an enzyme amount and a reaction time to calculate an enzyme activity. FIG. 7 is a graph illustrating an activity of the D-psicose 3-epimerase of the present invention for each substrate. In FIG. 7, the enzyme activity is relatively represented by setting an enzyme activity of a reaction of using psicose as a substrate to 100. As illustrated in FIG. 7, it was determined that the D-psicose 3-epimerase of the present invention had a high activity for psicose and fructose, and particularly, the activity of converting the fructose into the D-psicose was very high as compared with the existing D-psicose 3-epimerase. Therefore, the D-psicose can be produced from the fructose in a high yield by using the D-psicose 3-epimerase of the present invention.

(4) Analysis of thermal stability of D-psicose 3-epimerase



[0038] The D-psicose 3-epimerase obtained in Example 2 was prepared in a 50 mM PIPES buffer solution to be a concentration of 0.05 mg/ml and then, the obtained solutions were put and stored in water bathes set at temperatures of 55°C, 60°C, 65°C, and 70°C, respectively, to heat. A buffer solution of the purified enzyme was ejected for each stored time and treated with NiSO4 at a concentration of 1 mM, and then mixed with a 100 mM aqueous substrate solution at a weight ratio of 1:1 to prepare a test solution having pH 7.0, the enzyme concentration of 0.025 ml/mg, and the substrate concentration of 50 mM. Thereafter, the test solution reacted for 10 mins at 65°C and then a hydrochloric acid solution was added thereto to stop the reaction. Thereafter, the amount (mM) of produced psicose was analyzed and measured by the HPLC and divided into an enzyme amount and a reaction time to calculate an enzyme activity. As a result, even though the buffer solution of the purified enzyme was heated for 250 mins at 55°C, the enzyme activity was maintained as it is. Further, when the buffer solution of the purified enzyme was heated for about 200 mins at 60°C, the enzyme activity was reduced by about 80% as compared with before heat treatment. Generally, when it is considered that most of D-psicose 3-epimerase has a half-life (a heat treatment time when the enzyme activity is 50% as compared with before heat treatment) of approximately one hour at 50°C, the D-psicose 3-epimerase of the present invention has very excellent thermal stability.

(5) Analysis of activity of D-psicose 3-epimerase in high concentration of fructose



[0039] 700 µl of fructose at a concentration of 70 wt% was preheated at 60°C and added with 280 µl of a PIPES buffer solution at 0.1 mg/ml of a concentration of the D-psicose 3-epimerase obtained in Example 2 and then, the obtained reactant was reacted at 60°C. The reaction product was taken by 10 µl for each reaction time and diluted 25 times. A hydrochloric acid solution was added to the diluted product to stop the reaction. Thereafter, the amount (mM) of produced psicose was analyzed and measured by the HPLC and divided into an amount of the fructose used as the substrate to calculate a conversion rate. A conversion rate of fructose into psicose for each reaction time is listed in the following Table 3. As listed in Table 3, when the D-psicose 3-epimerase of the present invention reacted with the high-concentrated fructose, the conversion rate reached a maximum conversion rate exceeding 33% only for about 18 hrs.
[Table 3]
Reaction elapse time (hr)Conversion rate (%) of fructose into psicose
1 9.04
2 15.20
3 19.52
4 22.36
5 24.66
6 26.75
7 27.94
18 33.62
28 33.67


[0040] As described above, the present invention has been described through the Examples, but is not always limited thereto, and various modified embodiments can be made. Therefore, it should be interpreted that the scope of the present invention includes all embodiments belonging to claims accompanied in the present invention.

<110> DAESANG CORPORATION

<120> D-psicose 3-epimerase and manufacturing method of D-psicose using the same

<130> PCT-15-114

<150> 10-2014-0064651
<151> 2014-05-28

<160> 4

<170> KopatentIn 2.0

<210> 1
<211> 294
<212> PRT
<213> Artificial Sequence

<220>
<223> D-psicose 3-epimerase derived from Flavonifractor plautii

<400> 1



<210> 2
<211> 885
<212> DNA
<213> Artificial Sequence

<220>
<223> polynucleotide coding D-psicose 3-epimerase derived from Flavonifractor plautii

<400> 2

<210> 3
<211> 30
<212> DNA
<213> Artificial Sequence

<220>
<223> forward primer for cloning D-psicose 3-epimerase

<400> 3
cggcatatga acccgattgg aatgcactac   30

<210> 4
<211> 31
<212> DNA
<213> Artificial Sequence

<220>
<223> reverse primer for cloning D-psicose 3-epimerase

<400> 4
cggctcgagt tacgcggtca gctccttgag g   31




Claims

1. A method for producing psicose, the method comprising:

reacting fructose with a psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1, a recombinant strain, a culture of the recombinant strain, a lysate of the recombinant strain, or a composition including one or more thereof,

wherein the recombinant strain is transformed by a polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or a recombinant vector including the polynucleotide.


 
2. The method for producing psicose of claim 1, wherein the psicose epimerase is derived from Flavonifractor plautii.
 
3. The method for producing psicose of claim 1, wherein the psicose epimerase is produced by a method including the following steps of:

(a) expressing the psicose epimerase by culturing a recombinant strain which is transformed by a polynucleotide coding the psicose epimerase consisting of the amino acid sequence of SEQ ID NO: 1 or a recombinant vector including the polynucleotide; and

(b) isolating the psicose epimerase from a lysate of the recombinant strain with the expressed psicose epimerase.


 
4. The method for producing psicose of claim 3, wherein the polynucleotide consists of a base sequence of SEQ ID NO: 2.
 
5. The method for producing psicose of claim 3, wherein the step of isolating the psicose epimerase is performed by an affinity chromatography using a peptide tag.
 
6. The method for producing psicose of any one of claims 1 to 5, the composition further comprising: one or more kinds selected from the group consisting of manganese ions, nickel ions, and cobalt ions.
 
7. A method for producing psicose of any one of claims 1 to 5, wherein a temperature of the reaction is 55 to 67°C and a pH of the reaction is 6.5 to 8.
 
8. The method for producing psicose of any one of claims 1 to 5, wherein the concentration of the fructose is 35 to 75% (w/w).
 
9. The method for producing psicose of any one of claims 1 to 5, wherein the psicose epimerase or the recombinant strain is immobilized in a carrier.
 
10. The method for producing psicose of any one of claims 1 to 5, wherein the host strain of the recombinant strain is a cytologically safe strain.
 


Ansprüche

1. Verfahren zur Herstellung von Psicose, wobei das Verfahren umfasst:

Umsetzen von Fructose mit einer Psicose-Epimerase, bestehend aus der Aminosäuresequenz von SEQ ID NO: 1, einem rekombinanten Stamm, einer Kultur des rekombinanten Stammes, einem Lysat des rekombinanten Stammes oder einer Zusammensetzung, die eine oder mehrere davon enthält,

wobei der rekombinante Stamm durch ein Polynukleotid transformiert wird, das die Psicose-Epimerase kodiert, die aus der Aminosäuresequenz von SEQ ID NO: 1 oder einem rekombinanten Vektor besteht, der das Polynukleotid beinhaltet.


 
2. Verfahren zur Herstellung von Psicose nach Anspruch 1, wobei die Psicose-Epimerase von Flavonifraktor plautii abgeleitet ist.
 
3. Verfahren zur Herstellung von Psicose nach Anspruch 1, wobei die Psicose-Epimerase durch ein Verfahren hergestellt wird, das die folgenden Schritte umfasst:

(a) Exprimieren der Psicose-Epimerase durch Kultivieren eines rekombinanten Stammes, der durch ein Polynukleotid transformiert wird, das die Psicose-Epimerase kodiert, die aus der Aminosäuresequenz von SEQ ID NO: 1 oder einem rekombinanten Vektor einschließlich des Polynukleotids besteht, und

(b) Isolieren der Psicose-Epimerase aus einem Lysat des rekombinanten Stammes mit der exprimierten Psicose-Epimerase.


 
4. Verfahren zur Herstellung von Psicose nach Anspruch 3, wobei das Polynucleotid aus einer Basensequenz von SEQ ID NO: 2 besteht.
 
5. Verfahren zur Herstellung von Psicose nach Anspruch 3, wobei der Schritt der Isolierung der Psicose-Epimerase durch eine Affinitätschromatographie unter Verwendung eines Peptidtags durchgeführt wird.
 
6. Verfahren zur Herstellung von Psicose nach einem der Ansprüche 1 bis 5, wobei die Zusammensetzung ferner umfasst: eine oder mehrere Ionen, ausgewählt aus der Gruppe bestehend aus Manganionen, Nickelionen und Kobaltionen.
 
7. Verfahren zur Herstellung von Psicose nach einem der Ansprüche 1 bis 5, wobei eine Temperatur der Reaktion 55 bis 67°C und ein pH-Wert der Reaktion 6,5 bis 8 beträgt.
 
8. Verfahren zur Herstellung von Psicose nach einem der Ansprüche 1 bis 5, wobei die Konzentration der Fructose 35 bis 75 % (w/w) beträgt.
 
9. Verfahren zur Herstellung von Psicose nach einem der Ansprüche 1 bis 5, wobei die Psicose Epimerase oder der rekombinante Stamm in einem Träger immobilisiert wird.
 
10. Verfahren zur Herstellung von Psicose nach einem der Ansprüche 1 bis 5, wobei der Wirtsstamm des rekombinanten Stammes ein zytologisch sicherer Stamm ist.
 


Revendications

1. Procédé de production de psicose, le procédé comprenant :

la réaction du fructose avec une épimérase de psicose consistant en la séquence d'acides aminés de SEQ ID NO : 1, une souche recombinante, une culture de la souche recombinante, un lysat de la souche recombinante, ou une composition comprenant un ou plusieurs de ceux-ci,

la souche recombinante étant transformée par un polynucléotide codant pour l'épimérase du psicose consistant en la séquence d'acides aminés de SEQ ID NO : 1 ou un vecteur recombinant comprenant le polynucléotide.


 
2. Procédé de production de psicose selon la revendication 1, dans lequel l'épimérase de psicose est dérivée de Flavonifractor plautii.
 
3. Procédé de production de psicose selon la revendication 1, dans lequel l'épimérase de psicose est produite par un procédé comprenant les étapes suivantes de :

(a) exprimer l'épimérase du psicose en cultivant une souche recombinante qui est transformée par un polynucléotide codant pour l'épimérase du psicose consistant en la séquence d'acides aminés de SEQ ID NO : 1 ou un vecteur recombinant comprenant le polynucléotide, et

b) isolement de l'épimérase du psicose d'un lysat de la souche recombinante avec l'épimérase du psicose exprimée.


 
4. Procédé de production de psicose selon la revendication 3, dans lequel le polynucléotide consiste en une séquence de bases de SEQ ID NO : 2.
 
5. Procédé de production de psicose selon la revendication 3, dans lequel l'étape d'isolement de l'épimérase de psicose est réalisée par chromatographie d'affinité en utilisant un marqueur peptidique.
 
6. Procédé de production de psicose selon l'une quelconque des revendications 1 à 5, la composition comprenant en outre : un ou plusieurs ions choisis dans le groupe consistant en ions manganèse, ions nickel et ions cobalt.
 
7. Procédé de production de psicose selon l'une quelconque des revendications 1 à 5, dans lequel une température de la réaction est de 55 à 67°C et un pH de la réaction est de 6,5 à 8.
 
8. Procédé de production de psicose selon l'une quelconque des revendications 1 à 5, dans lequel la concentration du fructose est de 35 à 75% (p/p).
 
9. Procédé de production de psicose selon l'une quelconque des revendications 1 à 5, dans lequel l'épimérase de psicose ou la souche recombinante est immobilisée dans un support.
 
10. Procédé de production de psicose selon l'une quelconque des revendications 1 à 5, dans lequel la souche hôte de la souche recombinante est une souche cytologiquement sûre.
 




Drawing

















Cited references

REFERENCES CITED IN THE DESCRIPTION



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Patent documents cited in the description




Non-patent literature cited in the description