(19)
(11)EP 3 250 581 B1

(12)EUROPEAN PATENT SPECIFICATION

(45)Mention of the grant of the patent:
20.01.2021 Bulletin 2021/03

(21)Application number: 16701941.3

(22)Date of filing:  28.01.2016
(51)International Patent Classification (IPC): 
C07H 15/26(2006.01)
A61K 31/7056(2006.01)
C07H 13/08(2006.01)
C07H 19/056(2006.01)
A61P 11/00(2006.01)
C07H 13/10(2006.01)
(86)International application number:
PCT/EP2016/051836
(87)International publication number:
WO 2016/120403 (04.08.2016 Gazette  2016/31)

(54)

ALPHA-D-GALACTOSIDE INHIBITORS OF GALECTINS

ALPHA-D-GALACTOSIDINHIBITOREN VON GALECTINEN

INHIBITEURS DE ALPHA-D-GALACTOSIDE DE GALECTINES


(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30)Priority: 30.01.2015 EP 15153316
30.10.2015 EP 15192291

(43)Date of publication of application:
06.12.2017 Bulletin 2017/49

(73)Proprietor: Galecto Biotech AB
2200 Copenhagen (DK)

(72)Inventors:
  • BRIMERT, Thomas
    275 64 Blentarp (SE)
  • JOHNSSON, Richard
    245 41 Staffanstorp (SE)
  • LEFFLER, Hakon
    222 24 Lund (SE)
  • NILSSON, Ulf
    225 94 Lund (SE)
  • ZETTERBERG, Fredrik
    436 40 Askim (SE)

(74)Representative: Kjerrumgaard, Lars Bo 
LBKPATENT APS Cobis Ole Maaløes Vej 3
2200 Copenhagen
2200 Copenhagen (DK)


(56)References cited: : 
WO-A1-2005/113569
WO-A1-2010/126435
  
  • DENIS GIGURE ET AL: "Inhibitory potential of chemical substitutions at bioinspired sites of --galactopyranose on neoglycoprotein/cell surface binding of two classes of medically relevant lectins", BIOORGANIC & MEDICINAL CHEMISTRY, PERGAMON, GB, vol. 19, no. 10, 9 March 2011 (2011-03-09) , pages 3280-3287, XP028384652, ISSN: 0968-0896, DOI: 10.1016/J.BMC.2011.03.022 [retrieved on 2011-03-30]
  • RAJPUT VISHAL KUMAR ET AL: "Synthesis and evaluation of iminocoumaryl and coumaryl derivatized glycosides as galectin antagonists", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol. 24, no. 15, 9 June 2014 (2014-06-09), pages 3516-3520, XP028864112, ISSN: 0960-894X, DOI: 10.1016/J.BMCL.2014.05.063
 
Remarks:
The file contains technical information submitted after the application was filed and not included in this specification
 
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).


Description

Technical field



[0001] The present invention relates to novel compounds, the use of said compounds as medicament and for the manufacture of a medicament for the treatment of inflammation; fibrosis; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancers; autoimmune diseases; metabolic disorders; heart disease; heart failure; pathological angiogenesis; eye diseases; atherosclerosis; metabolic diseases; asthma and other interstitial lung diseases; and liver disorders in mammals. The invention also relates to pharmaceutical compositions comprising said novel compounds.

Background Art



[0002] Galectins are proteins with a characteristic carbohydrate recognition domain (CRD) (Barondes et al., 1994; Leffler et al., 2004). This is a tightly folded β-sandwich of about 130 amino acids (about 15 kDa) with the two defining features 1) a β -galactose binding site and 2) sufficient similarity in a sequence motif of about seven amino acids, most of which (about six residues) make up the β-galactose binding site. However, sites adjacent to the β -galactose site are required for tight binding of natural saccharides and different preferences of these give galectins different fine specificity for natural saccharides.

[0003] The recent completion of the human, mouse and rat genome sequences reveal about 15 galectins and galectin-like proteins in one mammalian genome with slight variation between species (Leffler et al., 2004)
Galectin subunits can contain either one or two CRDs within a single peptide chain. The first category, mono-CRDs galectins, can occur as monomers or dimers (two types) in vertebrates. The by far best studied galectins are the dimeric galectin-1, and galectin-3 that is a monomer in solution but may aggregate and become multimeric upon encounter with ligands (Leffler et al., 2004). These were the first discovered galectins and are abundant in many tissues.

[0004] There are now over 3500 publications on galectins in PubMed, with most, as mentioned above, about galectins-1 (>900) and -3 (>1600). Strong evidence suggests roles for galectins in e.g. inflammation and cancer, and development recently reviewed in a special issue (Leffler (editor), 2004b).

[0005] Galectins are synthesized as cytosolic proteins, without a signal peptide on free ribosomes. Their N-terminus is acetylated, a typical modification of cytosolic proteins, and they reside in the cytosol for a long time (not typical of secreted proteins). From there they can be targeted to the nucleus, specific cytososlic sites, or secreted (induced or constitutively) by a non-classical (non-ER-Golgi) pathway, as yet unknown, but possibly similar to the export of e.g. IL-1 (Leffler et al., 2004). They can also function in all these compartments; for galectin-3, solid evidence published in well respected journals support roles in RNA splicing in the nucleus, inhibition of apoptosis in the cytosol, and a variety of extracellular effects on cell signaling and adhesion (Leffler (editor), 2004b). Galectin-7 and -12 also act in the cytosol by enhancing apoptosis and regulating the cell cycle and differentiation in certain cells (Hsu and Liu in Leffler (editor), 2004b). Most galectins act also extracellularly by cross-linking glycoproteins (e.g. laminin, integrins, and IgE receptors) possibly forming supramolecular ordered arrays (Brewer et al., 2002) and may thereby modulate cell adhesion and induce intracellular signals. Related to this, recent years have seen the emergence of a molecular mechanism of these galectin functions involving a formation of microdomains (lattices) within membranes, (Dam et al., 2008; Garner et al., 2008) which in turn affects intracellular trafficking and cell surface presentation of glycoprotein receptors.(Delacour et al., 2007; Lau et al., 2007; Lau et al. 2008) This has been documented in cell culture, in null mutant mice,(Blois et al., 2007; Gedronneau et al., 2008; Thijssen et al., 2007; Toscano et al., 2007; Saegusa et al., 2009) and animals treated with galectin (Blois et al., 2007; Perone et al., 2009) or galectin inhibitors. (John et al., 2003; Pienta et al., 1995; Glinsky et al., 1996)

Potential therapeutic use of galectin-3 inhibitors



[0006] Galectin-3 has been implicated in diverse phenomena and, hence, inhibitors may have multiple uses. It is easy to perceive this as a lack of specificity or lack of scientific focus. Therefore, the analogy with aspirin and the cyclooxygenases (COX-I and II) is useful. The COXs produce the precursor of a wide variety of prostaglandins and, hence, are involved in a diverse array of biological mechanisms. Their inhibitors, aspirin and other NSAIDs (non-steroid anti-inflammatory drugs), also have broad and diverse effects. Despite this, these inhibitors are very useful medically, and they have several different specific utilities.

[0007] So if galectins, like COXs, are part of some basic biological regulatory mechanism (as yet unknown), they are likely to be 'used by nature' for different purpose in different contexts. Galectin inhibitors, like NSAIDs, are not expected to wipe out the whole system, but to tilt the balance a bit.

Inhibition of inflammation



[0008] A pro-inflammatory role of galectin-3 is indicated by its induction in cells at inflammatory sites, a variety of effects on immune cells (e.g. oxidative burst in neutrophils and chemotaxis in monocytes), and decrease of the inflammatory response, mainly in neutrophils and macrophages, in null mutant mice (in Leffler (editor), 2004b). Moreover, knock-out mice of Mac-2BP, a galectin-3 ligand, have increased inflammatory responses (Trahey et al., 1999). Importantly, recent studies have identified galectin-3 as a key rate-limiting factor in macrophage M2 differentiation and myofibroblast activation, which influences the development of fibrosis (Mackinnon et al., 2008; Mackinnon et al., 2012).

[0009] Inflammation is a protective response of the body to invading organisms and tissue injury. However, if unbalanced, frequently it is also destructive and occurs as part of the pathology in many diseases. Because of this, there is great medical interest in pharmacological modulation of inflammation. A galectin-3 inhibitor is expected to provide an important addition to the arsenal available for this.

Treatment of fibrosis-related conditions



[0010] The idea of a possible role of galectin-3 in fibrosis comes from cell and ex vivo studies on macrophage differentiation (Mackinnon et al., 2008), as well as from in vivo studies on macrophage differentiation and myofibroblast activation (Mackinnon et al., 2012). Briefly, the hypothesis is as follows: Galectin-3 has been shown to prolong cell surface residence and thus enhance responsiveness of the TGF-β receptor (Partridge et al., 2004), which in turn regulates alternative macrophage differentiation into M2 macrophages and myofibroblast activation.

[0011] Hence, as galectin-3 is a good candidate for being an endogenous enhancer of TGF-β signaling and alternative macrophage differentiation and myofibroblast activation, galectin-3 inhibitors may be very useful in treating fibrosis and adverse tissue remodeling.

Treatment of cancer



[0012] A large number of immunohistochemical studies show changed expression of certain galectins in cancer (van den Brule et. al. and Bidon et al. in Leffler (editor), 2004b) and for example galectin-3 is now an established histochemical marker of thyroid cancer. The direct evidence for a role of galectin-3 in cancer comes from mouse models, mainly by Raz et al, but also others (in Leffler (editor), 2004b). In paired tumor cell lines (with decreased or increased expression of galectin-3), the induction of galectin-3 gives more tumors and metastasis and suppression of galectin-3 gives less tumors and metastasis. Galectin-3 has been proposed to enhance tumor growth by being anti-apoptotic, promote angiogenesis, or to promote metastasis by affecting cell adhesion. Further, recent evidence have shown that galectin-3 plays a critical role in the tumor microenvironment - reviewed in Ruvolo, 2015. Galectin-3 is also believed to regulate the interaction between the tumor cells and immune cells, such as T-lymphocytes (T-cells), and inhibition of galectin-3 has been shown to restore T-cell activity (Demotte et al. 2010, Kouo et al. 2015, Menero et al. 2015). From the above it is clear that inhibitors of galectin-3 might have valuable anti-cancer effects. Indeed, saccharides claimed but not proven to inhibit galectin-3 have been reported to have anti-cancer effects. In our own study a fragment of galectin-3 containing the CRD inhibited breast cancer in a mouse model by acting as a dominant negative inhibitor (John et al., 2003). More recently, inhibition of galectin-3 with small molecules have been demonstrated to indeed greatly enhance tumor cell sensitivity towards radiation and standard pro-apoptotic drugs in cell assays and ex vivo (Lin et al., 2009), as well as in vivo (Glinsky et al., 2009).

[0013] Also galectin-1 is frequently over-expressed in low differentiated cancer cells, and galectin-9 or its relatives galectin-4 and galectin-8 may be induced in specific cancer types (Huflejt and Leffler, 2004; Leffler (editor), 2004b). Galectin-1 induces apoptosis in activated T-cells and has a remarkable immunosuppressive effect on autoimmune disease in vivo (Rabinovich et al; and Pace et al. in Leffler (editor), 2004b). Therefore, the over-expression of these galectins in cancers might help the tumor to defend itself against the T-cell response raised by the host.

[0014] Null mutant mice for galectins-1 and -3 have been established many years ago (Poirier, 2002). These are healthy and reproduce apparently normally in animal house conditions. However, recent studies have revealed subtle phenotypes in function of neutrophils and macrophages (as described above) and in bone formation for galectin-3 null mutants, and in nerve and muscle cell regeneration/differentiation for the galectin-1 null mutants (Leffler et al., 2004; Poirier, 2002; Watt in Leffler (editor), 2004b). Recently galectin-7 and galectin-9 null mutant mice have been generated and are also grossly healthy in animal house conditions, but have not yet been analyzed in detail. The differences in site of expression, specificity and other properties make it unlikely that different galectins can replace each other functionally. The observations in the null mutant mice would indicate that galectins are not essential for basic life supporting functions as can be observed in normal animal house conditions. Instead they may be optimizers of normal function and/or essential in stress conditions not found in animal house conditions. The lack of strong effect in null mutant mice may make galectin inhibitors more favorable as drugs. If galectin activity contributes to pathological conditions as suggested above but less to normal conditions, then inhibition of them will have less unwanted side effects.

Treatment of angiogenesis



[0015] Vascular endothelial growth factors (VEGFs) signaling through VEGF receptor-2 (VEGFR-2) is the primary angiogenic pathway. Studies have been published demonstrating that both galectin-1 and galectin-3 are important modulators for VEGF/VEGFR-2 signaling pathway. It has also been published that a galectin inhibitor, TDX, is expected have efficacy against pathological angiogenesis. (Chen 2012)

Known inhibitors


Natural ligands



[0016] Solid phase binding assays and inhibition assays have identified a number of saccharides and glycoconjugates with the ability to bind galectins (reviewed by Leffler, 2001 and Leffler et al., 2004). All galectins bind lactose with a Kd of 0.5 - 1 mM. The affinity of D-galactose is 50 - 100 times lower. N-Acetyllactosamine and related disaccharides bind about as well as lactose, but for certain galectins, they can bind either worse or up to 10 times better. The best small saccharide ligands for galectin-3 were those carrying blood group A-determinants attached to lactose or LacNAc-residues and were found to bind up to about 50 times better than lactose. Galectin-1 shows no preference for these saccharides.

[0017] Larger saccharides of the polylactosamine type have been proposed as preferred ligands for galectins. In solution, using polylactosamine-carrying glycopeptides, there was evidence for this for galectin-3, but not galectin-1 (Leffler and Barondes, 1986). A modified plant pectin polysaccharide has been reported to bind galectin-3 (Pienta et al., 1995).

[0018] The above-described natural saccharides that have been identified as galectin-3 ligands are not suitable for use as active components in pharmaceutical compositions, because they are susceptible to acidic hydrolysis in the stomach and to enzymatic degradation. In addition, natural saccharides are hydrophilic in nature, and are not readily absorbed from the gastrointestinal tract following oral administration.

Galectin specificity



[0019] The studies of galectin specificity using inhibition by small natural saccharides mentioned above indicated that all galectins bound lactose, LacNAc and related disaccharides, but that galectin-3 bound certain longer saccharides much better (Leffler and Barondes, 1986). These longer saccharides were characterized by having an additional sugar residue added to the C-3 position of galactose (in e.g. lactose or LacNAc) that bound an extended binding groove. The shape of this groove varies between galectins, suggesting that the same extensions would not be bound equally by the different galectins.

Synthetic inhibitors



[0020] Saccharides coupled to amino acids with anti-cancer activity were first identified as natural compounds in serum, but subsequently, synthetic analogues have been made (Glinsky et al., 1996). Among them, those with lactose or galactose coupled to the amino acid inhibit galectins, but only with about the same potency as the corresponding underivatized sugar. A chemically modified form of citrus pectin (Platt and Raz, 1992) that inhibits galectin-3 shows anti-tumor activity in vivo (Pienta et al., 1995; Nangia-Makker et al., 2002).

[0021] Cluster molecules having up to four lactose moieties showed a strong multivalency effect when binding to galectin-3, but not to galectin-1 and galectin-5 (Vrasidas et al., 2003). Cyclodextrin-based glycoclusters with seven galactose, lactose, or N-acetyllactosamine residues also showed a strong multivalency effect against galectin-3, but less so against galectins-1 and -7 (André et al., 2004). Starburst dendrimers (Andre et al., 1999) and glycopolymers (Pohl et al., 1999; David et al., 2004), made polyvalent in lactose-residues, have been described as galectin-3 inhibitors with marginally improved potency as compared to lactose. The aforementioned synthetic compounds that have been identified as galectin-3 ligands are not suitable for use as active components in pharmaceutical compositions, because they are hydrophilic in nature and are not readily absorbed from the gastrointestinal tract following oral administration.

[0022] Natural oligosaccharides, glycoclusters, glycodendrimers, and glycopolymers described above are too polar and too large to be absorbed and in some cases are large enough to produce immune responses in patients. Furthermore, they are susceptible to acidic hydrolysis in the stomach and to enzymatic hydrolysis. Thus, there is a need for small synthetic molecules.

[0023] Thiodigalactoside is known to be a synthetic and hydrolytically stable, yet polar inhibitor, approximately as efficient as N-acetyllactosamine (Leffler and Barondes, 1986). N-Acetyllactosamine derivatives carrying aromatic amides or substituted benzyl ethers at C-3' have been demonstrated to be highly efficient inhibitors of galectin-3, with unprecedented IC50 values as low as 4.8 µM, which is a 20-fold improvement in comparison with the natural N-acetyllactosamine disaccharide (Sörme et al., 2002; Sörme et al., 2003b). These derivatives are less polar overall, due to the presence of the aromatic amido moieties and are thus more suitable as agents for the inhibition of galectins in vivo. Furthermore, C3-triazolyl galactosides have been demonstrated to be as potent inhibitors as the corresponding C3-amides of some galectins. Hence, any properly structured galactose C3-substituent may confer enhanced galectin affinity.

[0024] However, the C3-amido- and C3-triazolyl-derivatised compounds are still susceptible to hydrolytic degradation in vivo, due to the presence of a glycosidic bond in the galactose and N-acetyllactosamine saccharide moiety and, although they are potent small molecule inhibitors of galectin-3, even further improved affinity and stability is desirable. Accordingly, inhibitors based on 3,3'-diamido- or 3,3'-ditriazolyl-derivatization of thiodigalactoside have been developed,(Cumpstey et al., 2005b; Cumpstey et al., 2008; Salameh et al., 2010; WO/2005/113569 and US2007185041; WO/2005/113568, US7,638,623 B2) which lack O-glycosidic hydrolytically and enzymatically labile linkages. These inhibitors also displayed superior affinity for several galectins (down to Kd in the low nM range). Nevertheless, although displaying high affinity for galectins, the 3,3'-derivatized thiodigalactosides still comprise a disadvantage in their multistep synthesis involving double inversion reaction to reach at 3-N-derivatized galactose building blocks. Furthermore, cyclohexane replacement of one galactose ring in thiodigalactoside has been evidenced to mimic the galactose ring and hence to provide galectin-1 and -3 inhibitors with efficiency approaching those of the diamido- and ditriazolyl-thiodigalactoside derivatives (WO/2010/126435). Replacement of a D-galactopyranose unit with a substituted cyclohexane decreases polarity and most likely also metabolic susceptibility, thus improving drug-like properties.

[0025] Some earlier described compounds have the following general formulas

as described in WO/2005/113568,
and

as described in WO/2005/113569, in which R1 can be a D-galactose.

[0026] In recently published US20140099319 and WO2014067986 are disclosed a compound of formula

having fluorine (F) in the meta position on both the phenyl rings in relation to the triazole rings. This compound has been shown to be a promising drug candidate for lung fibrosis, and in particular is very selective on galectin-3 with high affinity.

[0027] In Bioorganic & Medicinal Chemistry 19 (2011) 3280-3287 "Inhibitory potential of chemical substitutions at bioinspired sites of beta-D-galactopyranose on neoglycoprotein/cell surface binding of two classes of medically relevant lectins" are disclosed some beta-D-galactopyranosides with galectin-3 affinity in the same range or less lactose, which has a Kd of about 91 µM. There is no disclosure or mentioning of corresponding alpha-anomers having affinity towards galectin-3 better than lactose.

Summary of the invention



[0028] The compounds of the present invention are novel α-D-galactopyranose compounds that unexpectedly have shown very high affinity for galectin-3, and are considered novel potent drug candidates. Some of these compounds have very good PK properties for e.g. oral administration, such as low clearance and high bioavailability.

[0029] In broad aspect the present invention concerns a D-galactopyranose compound of formula (1)

wherein
the pyranose ring is α-D-galactopyranose,
A is selected from

wherein Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br; F; Cl; CN; NR19R20, wherein R19 and R20 are independently selected from H, C1-3 alkyl, cyclopropyl, iso-propyl, - C(=O)-R21, wherein R21 is selected from H and C1-3 alkyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; iso-propyl, optionally substituted with a F; O-cyclopropyl optionally substituted with a F; O-isopropyl optionally substituted with a F; and OC1-3 alkyl optionally substituted with a F;

wherein R1-R5 are independently selected from H, CN, NH2, F, methyl optionally substituted with a fluorine (F), and OCH3 optionally substituted with a F;

wherein R6 is selected from C1-6 alkyl optionally substituted with a halogen, branched C3-6 alkyl and C3-7 cycloalkyl;

X is selected from S, SO, SO2, O, C=O, and CR7R8 wherein R7 and R8 are independently selected from hydrogen, OH, or halogen (e.g. F, Cl, Br);

wherein R27 is selected from a C1-6 alkyl, branched C3-6 alkyl, C1-6 alkoxy and branched C3-6 alkoxy;

B is selected from a) a C1-6 alkyl or branched C3-6 alkyl substituted with a five or six membered heteroaromatic ring, optionally substituted with a substituent selected from CN, a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R14-CONH- wherein R14 is selected from C1-3 alkyl and cyclopropyl; or a C1-6 alkyl substituted with a phenyl, optionally substituted with a substituent selected from CN, a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R15-CONH- wherein R15 is selected from C1-3 alkyl and cyclopropyl; b) an aryl, such as phenyl or naphthyl, optionally substituted with a group selected from a halogen (e.g. Cl, F, Br, I); CN; -COOH; -CONR22R23, wherein R22 and R23 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; NR28R29, wherein R28 and R29 are independently selected from H, C1-3 alkyl and isopropyl; OH; and R16-CONH- wherein R16 is selected from C1-3 alkyl and cyclopropyl; c) a C5-7 cycloalkyl, optionally substituted with a substituent selected from a halogen (e.g. Cl, F, Br, I), CN, methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R17-CONH- wherein R17 is selected from C1-3 alkyl and cyclopropyl; and d) a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with a group selected from a halogen (e.g. Cl, F, Br, I); CN; -COOH; -CONR24R25, wherein R24 and R25 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; NR30R31, wherein R30 and R31 are independently selected from H, C1-3 alkyl and isopropyl; OH; and R18-CONH- wherein R18 is selected from C1-3 alkyl and cyclopropyl; e) a C1-6 alkyl or branched C3-6 alkyl; or a pharmaceutically acceptable salt or solvate thereof.



[0030] In a further aspect the present invention relates to a D-galactopyranose compound of formula (1)

wherein
the pyranose ring is α-D-galactopyranose,
A is selected from

wherein Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br; F; Cl; CN; NR19R20, wherein R19 and R20 are independently selected from H, C1-3 alkyl, cyclopropyl, iso-propyl, - C(=O)-R21, wherein R21 is selected from H and C1-3 alkyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; iso-propyl,optionally substituted with a F; O-cyclopropyl optionally substituted with a F;, O-isopropyl optionally substituted with a F; and OC1-3 alkyl optionally substituted with a F;

wherein R1-R5 are independently selected from H, CN, NH2, F, methyl optionally substituted with a fluorine (F), and OCH3 optionally substituted with a F;

wherein R6 is selected from C1-6 alkyl, branched C3-6 alkyl and C3-7 cycloalkyl;

B is selected from a) a C1-6 alkyl or branched C3-6 alkyl substituted with a five or six membered heteroaromatic ring, optionally substituted with a substituent selected from CN, a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R14-CONH- wherein R14 is selected from C1-3 alkyl and cyclopropyl or a C1-6 alkyl substituted with a phenyl, optionally substituted with a substituent selected from CN, a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R15-CONH- wherein R15 is selected from C1-3 alkyl and cyclopropyl; b) an aryl, such as phenyl or naphthyl, optionally substituted with a group selected from a halogen (e.g. Cl, F, Br, I); CN; -COOH; -CONR22R23, wherein R22 and R23 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; OH; and R16-CONH- wherein R16 is selected from C1-3 alkyl and cyclopropyl; c) a C5-7 cycloalkyl, optionally substituted with a substituent selected from a halogen (e.g. Cl, F, Br, I), CN, methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R17-CONH-wherein R17 is selected from C1-3 alkyl and cyclopropyl; and d) a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with a group selected from a halogen (e.g. Cl, F, Br, I), CN; -COOH; -CONR24R25, wherein R24 and R25 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; OH; and R18-CONH- wherein R18 is selected from C1-3 alkyl and cyclopropyl; or

a pharmaceutically acceptable salt or solvate thereof.

[0031] In a still further aspect the present invention relates to a D-galactopyranose compound of formula (1)

wherein
the pyranose ring is α-D-galactopyranose,
A is selected from

wherein Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F, and OCH3 optionally substituted with a F;

wherein R1-R5 are independently selected from H, CN, NH2, F, methyl optionally substituted with a F, and OCH3 optionally substituted with a F;

wherein R6 is selected from C1-6 alkyl, branched C3-6 alkyl and C3-7 cycloalkyl;

B is selected from a) a C1-6 alkyl or branched C3-6 alkyl substituted with a five or six membered heteroaromatic ring, optionally substituted with a substituent selected from CN, a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R14-CONH- wherein R14 is selected from C1-3 alkyl and cyclopropyl or a C1-6 alkyl substituted with a phenyl, optionally substituted with a substituent selected from CN, a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R15-CONH- wherein R15 is selected from C1-3 alkyl and cyclopropyl; b) an aryl, such as phenyl or naphthyl, optionally substituted with a substituent selected from a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R16-CONH-wherein R16 is selected from C1-3 alkyl and cyclopropyl; c) a C5-7 cycloalkyl, optionally substituted with a substituent selected from CN, a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R17-CONH- wherein R17 is selected from C1-3 alkyl and cyclopropyl; and d) a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with a substituent selected from a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R18-CONH- wherein R18 is selected from C1-3 alkyl and cyclopropyl; or

a pharmaceutically acceptable salt or solvate thereof.

[0032] In an embodiment of the present invention A is selected from formula 2 wherein R1-R5 are independently selected from H, F, methyl optionally substituted with a fluorine (F), and OCH3 optionally substituted with a F.

[0033] In another embodiment of the present invention A is selected from formula 2 wherein R1 and R5 are selected from H and R2-R4 are selected from F.

[0034] In a further embodiment of the present invention A is selected from formula 2 wherein R2 and R3 are F and R1, R4 and R5 are H, or wherein R2 and R4 are F and R1, R3 and R5 are H, or wherein R2 is F and R1, R3-R5 are H, or wherein R2 and R4 are F, R3 is OCH3, and R1 and R5 are H.

[0035] In a further embodiment of the present invention A is selected from formula 2 wherein R1-R5 are independently selected from H and F, provided that at least one of R1-R5 is F. Preferably from one to five, such as three or five, of R1-R5 are independently selected from F.

[0036] In a still further embodiment of the present invention A is selected from formula 3 wherein Het1 is selected from a six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, and Cl. Typically, Het1 is selected from a pyridinyl substituted with a F, such as 3 F.

[0037] In a further embodiment of the present invention A is selected from formula 4 wherein R6 is selected from C1-6 alkyl and branched C3-6 alkyl. Typically, R6 is C1-6 alkyl, such as CH3, CH2CH3, CH2CH2CH3 and isopropyl, e.g. CH3, or isopropyl.

[0038] In a further embodiment of the present invention A is selected from formula 4 wherein R6 is selected from C1-6 alkyl substituted with a halogen, such as one, two or three F, e.g. CH2CF3.

[0039] In a further embodiment of the present invention A is selected from formula 8 wherein R27 is selected from a C1-6 alkyl, branched C3-6 alkyl, C1-6 alkoxy and branched C3-6 alkoxy. Typically, R27 is selected from a C1-6 alkyl and C1-6 alkoxy.

[0040] In a further embodiment of the present invention X is selected from S, SO, SO2, and O. Preferably X is selected from S, SO, and SO2. In a further embodiment X is selected from S and SO2, such as S.

[0041] In a still further embodiment of the present invention B is selected from a C1-6 alkyl substituted with a phenyl, optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F, and OCH3 optionally substituted with a F.

[0042] In a further embodiment of the present invention B is selected from a C1-6 alkyl substituted with a phenyl, such as benzyl or CH2-CH2-phenyl.

[0043] In a still further embodiment of the present invention B is selected from a C1-6 alkyl substituted with a phenyl, said phenyl substituted with a group selected from Cl, such as benzyl substituted with one or two Cl, or -CH2-CH2-phenyl substituted with one Cl.

[0044] In a further embodiment of the present invention B is selected from a C1-6 alkyl.

[0045] In a still further embodiment of the present invention B is selected from an aryl, such as phenyl or naphthyl, optionally substituted with one or more substituents selected from a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R16-CONH- wherein R16 is selected from C1-3 alkyl and cyclopropyl.

[0046] In a further embodiment of the present invention B is selected from an aryl, such as phenyl or naphthyl, optionally substituted with one or more substituents selected from I, COOH and CONH2.

[0047] In a further embodiment of the present invention B is selected from an aryl, such as phenyl or naphthyl, optionally substituted with one or more substituents selected from NR28R29, wherein R28 and R29 are independently selected from H, C1-3 alkyl and isopropyl.

[0048] In a still further embodiment of the present invention B is selected from an aryl, such as phenyl or naphthyl, optionally substituted with one or more substituents selected from CN.

[0049] In a further embodiment of the present invention B is selected from an unsubstituted phenyl.

[0050] In a still further embodiment of the present invention B is selected from an unsubstituted naphthyl.

[0051] In a further embodiment of the present invention B is selected from a phenyl substituted with one, two or three substituents selected from Cl, F, Br, CN, methyl, OH, CF3, OCH2CH3, OCH3, OCF3, R16-CONH- wherein R16 is selected from C1-3 alkyl, such as methyl. Such as a phenyl substituted with two substituents selected from Cl, F, Br, CN. Such as a phenyl substituted with three substituents selected from Cl, F, and CN.

[0052] In a still further embodiment of the present invention B is selected from a phenyl substituted with one, two or three substituents selected from Cl, F, Br, I CN, methyl, OH, CF3, OCH2CH3, OCH3, OCF3, COOH, CONH2, and R16-CONH- wherein R16 is selected from C1-3 alkyl, such as methyl. Such as a phenyl substituted with one substituent selected from I, COOH, and CONH2.

[0053] In a further embodiment of the present invention B is selected from a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with one or more substituents selected from a halogen (e.g. Cl, F, Br, I); methyl optionally substituted with a F; OCH3 optionally substituted with a F; OCH2CH3 optionally substituted with a F; CONH2; OH; NR30R31, wherein R30 and R31 are independently selected from H, C1-3 alkyl and isopropyl; and R18-CONH- wherein R18 is selected from C1-3 alkyl and cyclopropyl.

[0054] In a still further embodiment of the present invention B is selected from a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with one or more substituents selected from a halogen (e.g. Cl, F, Br, I); methyl optionally substituted with a F; OCH3 optionally substituted with a F; OCH2CH3 optionally substituted with a F; OH; and R18-CONH- wherein R18 is selected from C1-3 alkyl and cyclopropyl.

[0055] In a further embodiment of the present invention B is selected from a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with one or more substituents selected from CONH2; OH; Cl; Br; and CF3.

[0056] In a still further embodiment of the present invention B is selected from a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with one or more substituents selected from CN.

[0057] In a further embodiment of the present invention B is selected from a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with one to three substituents selected from CONH2; OH; CN; Cl; Br; and CF3.

[0058] In a still further embodiment of the present invention B is selected from an unsubstituted pyridinyl.

[0059] In a further embodiment of the present invention B is selected from a pyridinyl substituted with one to three, such as two, substituents selected from Cl, Br, CF3 and CN.

[0060] In a still further embodiment of the present invention B is selected from a pyridinyl substituted with one to three, such as one or two, substituents selected from CONH2, and OH.

[0061] In a further embodiment of the present invention B is selected from a pyridinyl substituted with one to three, such as one, substituents selected from NR30R31, wherein R30 and R31 are independently selected from H, C1-3 alkyl and isopropyl.

[0062] In a further embodiment of the present invention B is selected from a pyridazinyl substituted with one to three, such as two, substituents selected from Cl, OH, OCH3, and CN.

[0063] In a still further embodiment of the present invention B is selected from a thiophenyl substituted with one to three, such as one, substituents selected from halogen, such as Cl.

[0064] In a further embodiment of the present invention B is selected from a C5-7 cycloalkyl, optionally substituted with one or more substituents selected from a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R17-CONH-wherein R17 is selected from C1-3 alkyl and cyclopropyl.

[0065] In a still further embodiment of the present invention B is a cyclohexyl optionally substituted with one or more substituents selected from a halogen.

[0066] In a further embodiment of the present invention B is a cyclohexyl.

[0067] In a still further embodiment of the present invention B is a cyclohexyl substituted with one or two substituents selected from a halogen, such as 2 F.

[0068] As mentioned above some of the compounds of the present invention have high galectin-3 affinity and very good PK properties, showing high oral bioavailability and are suitable for oral administration, and the data presented herein supports that at least the compounds of formula (1) of the present invention wherein A is selected from formula 2 wherein R1-R5 are independently selected from H, F, methyl optionally substituted with a F, and OCH3 optionally substituted with a F, typically R1-R5 are selected from H, F, Br, and Cl, such as R2-R4 are selected from F, Br, and Cl, and R1 and R5 are H or R1-R5 are all selected from F, Br, and Cl; preferably, R1-R5 are selected from H and F, such as R2-R4 are selected from F, and R1 and R5 are H or R1-R5 are all selected from F; wherein X is selected from S and SO2, and wherein B is selected from a phenyl substituted with one, two or three substituents selected from Cl, F, Br, methyl, OH, CN, CF3, OCH2CH3, OCH3, OCF3, R16-CONH- wherein R16 is selected from C1-3 alkyl, such as methyl, typically B is phenyl substituted with 1-3 substituents selected from Cl, F, CN, and Br, such as Cl in meta or para position or both, F in ortho, meta or para position or all three, CN in meta position, or Br in meta or para position or both, or two or three selected from Cl, F, CN, and Br in ortho, meta and para position; or wherein B is selected from a pyridinyl and pyridazinyl substituted with one, two or three substituents selected from Cl, F, Br, OH, CN, CF3, OCH2CH3, OCH3, OCF3, typically B is selected from a pyridinyl and pyridazinyl substituted in meta and para position with two substituents selected from Cl, Br, OH, CN, CF3, OCH3; have these very good PK properties and high galectin-3 affinity.

[0069] In a further embodiment of the present invention, the compound is selected from any one of examples 1-42:

3,4-Dimethylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Ethoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Pyridyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Acetanilidyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Methoxyphenyl 3 -deoxy- -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranoside,

2,3-Dichlorophenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Benzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Methoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Naphtyl3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Methylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-(Trifluoromethyl)phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,5-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2,6-Dimethylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

1-Naphthyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-(Trifluoromethoxy)phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Pyridyl 3-deoxy-3-[4-(3,4,5 -trifluorophenyl -1H-1,2,3-triazol-1-yl]-)-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

Phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Tolyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Trifluoromethoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

Cyclohexyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2,4,5-Trichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2,5-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Hydroxy-phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

2-Phenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, and

3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide.



[0070] In a further embodiment of the present invention the compound is selected from any one of examples 43-55:

5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-5-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-6-fluoro-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromo-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Bromo-6-trifluoromethyl-pyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-cyano-pyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-cyano-pyridazin-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

5-Methoxy-6-cyano-pyridazin-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, and

5-Hydroxy-6-cyano-pyridazin-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,



[0071] In a further embodiment of the present invention the compound is selected from any one of examples 56-106:

3-Chloro-2,4-difluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy -3-[4-(phenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,5-Dichloro-4-fluorophenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichloro-6-fluorophenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromo-4-fluorophenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-4-(trifluoromethyl)phenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3,4,5-Trichlorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

5-Chloro-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

5-Bromo-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

5-Chloro-2-methoxyphenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3-Iodophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

Picolinamide-5-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Cyanophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

2-Cyanopyridine-5-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Chloro-2-thienyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Carboxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Benzamide-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3-fluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,3'-difluoro-cyklohexyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

n-Butyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,5-difluoro-4-methoxyphenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Hydroxy-pyridin-4-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Chlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3,4-Dichlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Chlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

Propyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Aminopyridin-4-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-dimethylamino-naphatlen-2-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Ethyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

S-5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

R-5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

S-Chloropyiidin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

R-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

5-Dimethylamino-naphtalen-2-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

3,4-Dichlorophenyl 3-deoxy-3-[4-(ethoxycarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(ethylaminocarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(propyl-carbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-trifluoromethyl-pyridine-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-2-trifluoromethyl-pyridine-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-cyano-pyridine-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, and

3-Chloro-4-cyanophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside.



[0072] In a further aspect the present invention relates to a compound of formula (1) for use as a medicine.

[0073] In a still further aspect the present invention relates to a pharmaceutical composition comprising the compound of any one of the previous claims and optionally a pharmaceutically acceptable additive, such as a carrier and/or excipient.

[0074] In a further aspect the present invention relates to a compound of formula (1) of the present invention for use in a method for treating a disorder relating to the binding of a galectin-3 to a ligand in a mammal, such as a human. In a further embodiment the disorder is selected from the group consisting of inflammation; fibrosis, such as pulmonary fibrosis, liver fibrosis, kidney fibrosis, ophthalmological fibrosis and fibrosis of the skin and heart; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancer, such as carcinomas, sarcomas, leukemias and lymphomas, such as T-cell lymphomas; metastasising cancers; autoimmune diseases, such as psoriasis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, ankylosing spondylitis, systemic lupus erythematosus; metabolic disorders; heart disease; heart failure; pathological angiogenesis, such as ocular angiogenesis or a disease or condition associated with ocular angiogenesis, e.g. neovascularization related to cancer; and eye diseases, such as age-related macular degeneration and corneal neovascularization; atherosclerosis; metabolic diseases such as diabetes; asthma and other interstitial lung diseases, including Hermansky-Pudlak syndrome, mesothelioma; liver disorders, such as non-alcoholic steatohepatitis.

[0075] A compound of formula (1) of the present invention may be used in combination therapy together with a therapeutically active compound different from the compound of formula (1) (interchangeable with "a different therapeutically active compound"). In particular a combination of a compound of formula (1) and a different therapeutically active compound for use in treatment of a disorder relating to the binding of a galectin-3 to a ligand in a mammal. Such disorders are disclosed below.

[0076] A therapeutically effective amount of at least one compound of formula (1) of the present invention may be administered to a mammal in need thereof in combination with a different therapeutically active compound. Said combination of a compound of formula (1) together with a different therapeutically active compound is administered to a mammal suffering from a disorder selected from the group consisting of inflammation; fibrosis, such as pulmonary fibrosis, liver fibrosis, kidney fibrosis, ophthalmological fibrosis and fibrosis of the skin and heart; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancer, such as carcinomas, sarcomas, leukemias and lymphomas, such as T-cell lymphomas; metastasising cancers; autoimmune diseases, such as psoriasis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, ankylosing spondylitis, systemic lupus erythematosus; metabolic disorders; heart disease; heart failure; pathological angiogenesis, such as ocular angiogenesis or a disease or condition associated with ocular angiogenesis, e.g. neovascularization related to cancer; and eye diseases, such as age-related macular degeneration and corneal neovascularization; atherosclerosis; metabolic diseases such as diabetes; asthma and other interstitial lung diseases, including Hermansky-Pudlak syndrome, mesothelioma; liver disorders, such as non-alcoholic steatohepatitis.

[0077] A non-limiting group of cancers given as examples of cancers that may be treated, managed and/or prevented by administration of a compound of formula (1) in combination with a different therapeutically active compound is selected from: colon carcinoma, breast cancer, pancreatic cancer, ovarian cancer, prostate cancer, fibrosarcoma, myxosarcoma, liposarcoma, chondrosarcoma, osteogenic sarcoma, chordoma, angiosarcoma, endotheliosarcoma, lymphangeosarcoma, lymphangeoendothelia sarcoma, synovioma, mesothelioma, Ewing's sarcoma, leiomyosarcoma, rhabdomyosarcoma, squamous cell carcinoma, basal cell carcinoma, adenocarcinoma, sweat gland carcinoma, sebaceous gland carcinoma, papillary carcinoma, papillary adenocarcinomas, cystandeocarcinoma, medullary carcinoma, bronchogenic carcinoma, renal cell carcinoma, hepatoma, bile duct carcinoma, choriocarcinoma, seminoma, embryonal carcinoma, Wilms' tumor, cervical cancer, testicular tumor, lung carcinoma, small cell lung carcinoma, bladder carcinoma, epithelial carcinoma, glioblastomas, neuronomas, craniopharingiomas, schwannomas, glioma, astrocytoma, medulloblastoma, craniopharyngioma, ependymoma, pinealoma, hemangioblastoma, acoustic neuroama, oligodendroglioma, meningioma, melanoma, neuroblastoma, retinoblastoma, leukemias and lymphomas, acute lymphocytic leukemia and acute myelocytic polycythemia vera, multiple myeloma, Waldenstrom's macroglobulinemia, and heavy chain disease, acute nonlymphocytic leukemias, chronic lymphocytic leukemia, chronic myelogenous leukemia, Hodgkin's Disease, non-Hodgkin's lymphomas, rectum cancer, urinary cancers, uterine cancers, oral cancers, skin cancers, stomach cancer, brain tumors, liver cancer, laryngeal cancer, esophageal cancer, mammary tumors, childhood-null acute lymphoid leukemia (ALL), thymic ALL, B-cell ALL, acute myeloid leukemia, myelomonocytoid leukemia, acute megakaryocytoid leukemia, Burkitt's lymphoma, acute myeloid leukemia, chronic myeloid leukemia, and T cell leukemia, small and large non-small cell lung carcinoma, acute granulocytic leukemia, germ cell tumors, endometrial cancer, gastric cancer, cancer of the head and neck, chronic lymphoid leukemia, hairy cell leukemia and thyroid cancer.

[0078] Combination therapy may involve administering a compound of formula (1) of the present invention together with an anti-fibrotic compound different form the compound of formula (1) to a mammal in need thereof. Such anti-fibrotic compound may be selected from the following non-limiting group of anti-fibrotic compounds: pirfenidone, nintedanib, simtuzumab (GS-6624, AB0024), BG00011 (STX100), PRM-151, PRM-167, PEG-FGF21, BMS-986020, FG-3019, MN-001, IW001, SAR156597, GSK2126458, and PBI-4050.

[0079] Combination therapy may involve administering a compound of formula (1) in combination with a further conventional cancer treatment such as chemotherapy or radiotherapy, or treatment with immunostimulating substances, gene therapy, treatment with antibodies and treatment using dendritic cells, to a mammal in need thereof.

[0080] The compound of formula (1) may be administered together with at least one additional therapeutic agent selected from an antineoplastic chemotherapy agent. The antineoplastic chemotherapeutic agent is selected from: all-trans retinoic acid, Actimide, Azacitidine, Azathioprine, Bleomycin, Carboplatin, Capecitabine, Cisplatin, Chlorambucil, Cyclophosphamide, Cytarabine, Daunorubicin, Docetaxel, Doxifluridine, Doxorubicin, Epirubicin, Etoposide, Fludarabine, Fluorouracil, Gemcitabine, Hydroxyurea, Idarubicin, Irinotecan, Lenalidomide, Leucovorin, Mechlorethamine, Melphalan, Mercaptopurine, Methotrexate, Mitoxantrone, Oxaliplatin, Paclitaxel, Pemetrexed, Revlimid, Temozolomide, Teniposide, Thioguanine, Valrubicin, Vinblastine, Vincristine, Vindesine and Vinorelbine. In one embodiment, a chemotherapeutic agent for use in the combination of the present agent may, itself, be a combination of different chemotherapeutic agents. Suitable combinations include FOLFOX and IFL. FOLFOX is a combination which includes 5-fluorouracil (5-FU), leucovorin, and oxaliplatin. IFL treatment includes irinotecan, 5-FU, and leucovorin.

[0081] The further conventional cancer treatment includes radiation therapy.Radiation therapy includes localized radiation therapy delivered to the tumor.Radiation therapy includes total body irradiation.

[0082] Cancer treatment is selected from the group of immunostimulating substances e.g. cytokines and antibodies. Such cytokines may be selected from the group consisting of, but not limited to: GM-CSF, type I IFN, interleukin 21, interleukin 2, interleukin 12 and interleukin 15. The antibody is preferably an immunostimulating antibody such as anti-CD40 or anti-CTLA-4 antibodies. The immunostimulatory substance may also be a substance capable of depletion of immune inhibitory cells (e.g. regulatory T-cells) or factors, said substance may for example be E3 ubiquitin ligases. E3 ubiquitin ligases (the HECT, RING and U-box proteins) have emerged as key molecular regulators of immune cell function, and each may be involved in the regulation of immune responses during infection by targeting specific inhibitory molecules for proteolytic destruction. Several HECT and RING E3 proteins have now also been linked to the induction and maintenance of immune self-tolerance: c-Cbl, Cbl-b, GRAIL, Itch and Nedd4 each negatively regulate T cell growth factor production and proliferation.

[0083] The compound of formula (1) may be administered together with at least one additional therapeutic agent selected from a checkpoint inhibitor.The checkpoint inhibitor is acting on one or more of the following, non-limiting group of targets: CEACAM1, galectin-9, TIM3, CD80, CTLA4, PD-1, PD-L1, HVEM, BTLA, CD160, VISTA, B7-H4, B7-2, CD155, CD226, TIGIT, CD96, LAG3, GITF, OX40, CD137, CD40, IDO, and TDO. These are known targets and some of these targets are described in Melero et al., Nature Reviews Cancer (2015).

[0084] The compound of formula (1) may be administered together with at least one additional therapeutic agent selected from an inhibitor of indoleamine-2,3-dioxygenase (IDO).

[0085] The compound of formula (1) may be administered together with at least one additional therapeutic agent selected from one or more inhibitors of the CTLA4 pathway.The inhibitor of the CTLA4 pathway is selected from one or more antibodies against CTLA4.

[0086] The compound of formula (1) may be administered together with at least one additional therapeutic agent selected from one or more inhibitors of the PD-1/PD-L pathway.The one or more inhibitors of the PD-1/PD-L pathway are selected from one or more antibodies against PD-1, PD-L1, and/or PD-L2.

[0087] The compounds of the present invention may be prepared by a process of preparing a compound of formula III or a pharmaceutically acceptable salt or solvate thereof comprising the step a1;

W= Het1-,

R6NHC(O)- or COOP1
a1) Reacting the compound of formula I wherein X and B are as defined above under formula (1), with a compound of formula II in an inert solvent, such as DMF or acetonitrile, using a base, such as diisopropylethylamine, catalyzed by CuI to provide the compound of the formula III.

[0088] The compounds of the present invention may be prepared by a process of preparing a compound of formula V or a pharmaceutically acceptable salt or solvate thereof comprising the step a2;

a2) reacting I with IV, wherein P1 is selected from a C1-5 alkyl group, in an inert solvent, such as DMF or acetonitrile, using a base, such as diisopropyletylamine, catalyzed by CuI to give a compound which upon treatment with a aliphatic base, such as R6-NH2 yields a compound of formula V.

[0089] The compounds of the present invention may be prepared by a process of preparing a compound of formula VII and/or VIII or a pharmaceutically acceptable salt or solvate thereof comprising the step a3;

W= Het1-,

R6NHC(O)- or COOP1
a3) reacting a compound of formula VI with an oxidant such as hydrogen peroxide in a solvent such as acetic acid, alternatively 3-chloroperoxybenzoic acid in an inert solvent such as dichloromethane to give a compound of formula VII and/or VIII.

[0090] A reference compound of the present invention may be prepared by a process of preparing a compound of formula X wherein R7 is as defined above under formula (1), or a pharmaceutically acceptable salt or solvate thereof comprising the step a4;

a4) Reacting a compound of the formula IX with guanidine hydrochloride in the presence of potassium carbonate in an inert solvent such as tetrahydrofuran, to give a compound of formula X.

[0091] Other reference compounds of the present invention may be prepared by a process of preparing a compound of formula XII or a pharmaceutically acceptable salt or solvate thereof comprising the step a5;

a5) Reacting a compound of formula XI with a reagent such as sodium methoxide in methanol give a compound of formula XII.

[0092] The compounds of the present invention may be prepared by a process of preparing a compound of formula XV or a pharmaceutically acceptable salt or solvate thereof comprising the steps a6;

a6) Reacting a compound of the formula XIII with a compound of the formula XIV using a reagents such as HATU in the presence of a base such as didisopropylethylamine (DIPEA) in an inert solvent such as DMF to give a compound of formula XV.

[0093] The compounds of the present invention may be prepared by a process of preparing a compound of formula I wherein X is defined as oxygen or sulfur or a pharmaceutically acceptable salt or solvate thereof comprising the steps a7 and a8;

a7) Reacting a compound XVI with a chlorinating reagent such as dichloromethylmethylether or PCl5 in the presence of a lewis acid such as BF3 Et2O in an inert solvent as dichloromethane or chloroform to give a compound of formula XVII.

a8) Reacting a compound of the formula XVII with a nucleophile like B-XH, wherein X is defined as sulfur or oxygen, in the presence of a base like sodium hydride in an inert solvent such as DMF.



[0094] The compounds of the present invention may be prepared by a process of preparing a compound of formula I wherein X is defined as CH2 or a pharmaceutically acceptable salt or solvate thereof comprising the steps a9-a13;

a9) A Compound XVII which can be prepared using the methods described in Chem. Eur. J. 2009, 15, 2861-2873 can be reacted with a nucleophile such as the Grignard reagent B-MgBr in an inert solvent such as ether to give a compound of formula XVIII.

a10) A compound of the formula XVIII could be transformed into a compound of the formula XIX using a reagent such as (ClCH2O)2 in the presence of a base such as pyridine.

a11) A compound of the formula XIX could be transformed to a compound of formula XX using hydrogen gas in the presence of a catalyst such as palladium on carbon in an inert solvent such as methanol.

a12) A compound of the formula XX could be selectively alkylated at the 3-hydroxy position by treatment of dibutyltinoxide followed by benzylbromide. The reamining hydroxy groups could then be protected using other protective groups which are stable under conditions for removing the benzyl, these include acetoxy, silyl and orthesters. The benzyl group is then selectively removed and the free hydroxy group in the 3 position is inverted using e.g. triflic anhydride in the presence of pyridine followed by Bu2NNO2 in an inert solvent such as DMF to give compound XXI

a13) A compound of the formula XXI could be reacted with a reagent such as tosylchloride in the presence of a base such as pyridine to give a compound which could be reacted with sodium azide in an inert solvent such as DMF to give a compound of the formula XXII.



[0095] The compounds of the present invention may be prepared by a process of preparing a compound of formula XXV wherein X is as defined above under formula (1), or a pharmaceutically acceptable salt or solvate thereof comprising the steps a14-16;

a14) A compound of the formula XVIII could be treated with a halide formation reagent such as DAST(fluorine), PCl5(chlorine), PBrs (Bromine) to give a compound XXIII wherein W1 is the corresponing halide F, Cl or Br and W2 is hydrogen.
Alternatively XVIII could be treated with an oxidising reagent such as PCC to give a compound XXIII where W1 and W2 together form a bond to oxygen. This compound could be treated with a halide formation reagent such as DAST(fluorine), PCl5(chlorine), PBr5 (Bromine) to give a compound XXIII wherein W1 = W2 is the corresponing halide F, Cl or Br.

a15) A compound of the formula XXIII could be selectively alkylated at the 3-hydroxy position by treatment of dibutyltinoxide followed by benzylbromide. The reamining hydroxy groups could then be protected using other protective groups which are stable under conditions for removing the benzyl, these include acetoxy, silyl and orthesters. The benzyl group is then selectively removed and the free hydroxy group in the 3 position is inverted using e.g. triflic anhydride in the presence of pyridine followed by Bu2NNO2 in an inert solvent such as DMF to give compound XXIV.

a16) A compound of the formula XXIV could be reacted with a reagent such as tosylchloride in the presence of a base such as pyridine to give a compound which could be reacted with sodium azide in an inert solvent such as DMF to give a compound of the formula XXV.



[0096] Other reference compounds of the present invention may be prepared by a process of preparing a compound of formula XI wherein R8, R9, R10, R11, R12, X and B are defined as in formula (1) above, or a pharmaceutically acceptable salt or solvate thereof comprising the steps a17;

a17) A compound of the formula XXVII could be reacted with a compound of XXVII in the presence of toluenesulfoneazide, cupper iodide, triethyl amine in a solvent such as THF to give a compound of formula XI wherein R12 is defined as H.

[0097] The compounds of the present invention may be prepared by a process of preparing a compound of formula IX wherein R7, X and B are defined as in formula (1) above, or a pharmaceutically acceptable salt or solvate thereof comprising the step a18;

a18) A compound of formula XXVII could be reacted with a compound of formula R7-COCl in the presence of CuI, PdCl2(PPh3)2Cl2 and triethylamine in an inert solvent such as THF to give a compound of formula IX.

[0098] The compounds of the present invention may be prepared by a process of preparing a compound of formula XXVII, wherein X are defined as oxygen or sulfur and B is defined as above in formula (1), or a pharmaceutically acceptable salt or solvate thereof comprising the steps a19-a20;

a19) Reacting a compound of the formula XXV, prepared in according to Farkas, I.; Szabó, I. F.; Bognár, R.; Anderle, D. Carbohydr. Res. 1976, 48, 136-138 or Ibatullin, F. M.; Selivanov, S. I.. Tetrahedron Letters 2002, 43, 9577-9580, with a nucleophile such as B-XH, wherein X is defined as sulfur or oxygen, in the presence of a base like sodium hydride in an inert solvent such as DMF to give a compound of formula XXVI.

a20) Reacting a compound of the formula XXVI with dibutyltin oxide in an inert solvent such as methanol to give a compound which is further reacted with a 3-bromoprop-1-yne in the presence of tetrabutylammonium iodide in an inert solvent such as tetrahydrofuran to give a compound of formula XXVII.



[0099] The compounds of the present invention may be prepared by a process of preparing a compound of formula II comprising the step a21: W-L

XXVIII
W= Het1-,

a21) Reacting a compound of formula XXVIII wherein P1 is defined as a C1-5 alkyl group, such as methyl and L is defined as a leaving group such as bromine, with trimethylsilane-acetylene using a palladium catalyst such as bis(triphenylphosphine)palladium-(II)-chloride, copper iodide and a base like diisopropylethylamine in an inert solvent, such as THF, to give a compound of formula II.

[0100] The compounds of the present invention may be prepared by a process of preparing a compound of the formula XXIX comprising step a22)
a22)

W= Het1-,

R6NHC(O)- or COOP1 Reacting a compound of the formula XXIIX with a compound of the formula B-L, wherein L is defined as a leaving group such as Chlorine, in an inert solvent as DMF using a base such as sodium hydride to give a compound of the formula XXIX.

[0101] The compounds of the present invention may be prepared by a process of preparing a compound of the formula XXIIX comprising step a23-a25)

a23) Reacting the compound of formula XVI, with a compound of formula II in an inert solvent, such as DMF or acetonitrile, using a base, such as diisopropylethylamine, catalyzed by CuI to provide the compound of the formula XXX.

a24) Reacting a compound XXX with a chlorinating reagent such as dichloromethylmethylether or PCI5 in the presence of a lewis acid such as BF3 Et2O in an inert solvent as dichloromethane or chloroform to give a compound of formula XXXI.

a25) Reacting a compound of formula XXXI with a sulfurus nucleophile such as thioacetate followed by deprotection using a base such as sodium methoxide, to give XXIIX.



[0102] The compounds of the present invention may be prepared by a process of preparing a compound of the formula XXXV comprising step a26-a28

a26) Reacting a compound of the formula XXXII with an activated thioamide such as dimethylcarbamoyl chloride using a base such as sodium hydride in an inert solvent such as DMF to give a compound of formula XXXIII.

a27) Heating a compound of the formula XXXIII at elevated temperatures to form compound XXXIV.

a28) Reacting a compound of formula XXXIV with a base such as potassium hydroxide to give a compound of the formula XXXV.



[0103] The compounds of the present invention may be prepared by a process of preparing a compound of the formula XXXV comprising step a29-a30

a29) A compound of the formula XXXVI could upon treatment with sodium nitrite form the corresponding diazocompound. This compound could be further reacted with a sulfurus source such as potassium Ethyl xantogenate to form the compound XXXVII.

a30) Reacting a compound of formula XXXVII with a base such as potassium hydroxide to give a compound of formula XXXV.



[0104] The compounds of the present invention may be prepared by a process of preparing a compound of the formula XLI comprising step a31-a34

a31) Reacting a compound of formula XXXVIII wherein P2 is defined an C1-6 alkyl with a diazotizing agent such as N-(4-azidosulfonylphenyl)acetamide in an inert solvent such as acetonitrile, optionally in the prescence of a base such as triethyl amine to give XXXIIX.

a32) Reacting a Compound of the formula XXXIIX with triphenylphosphine, followed by subsequent reaction with acetic acid to give a compound of formula XXXIX.

a33) Reacting a compound of the formula XXXIX with a solution of ammonia in an alcohol such as methanol to give compound XL.

a34) Reacting a compound of formula XL with a reagent such a POCl3 or POBr3 to give a compound of the formula XLI, wherein L is defined as a halide such as chlorine or bromine.



[0105] The compounds of the present invention may be prepared by a process of preparing a compound of the formula XLIV comprising step a35-a36

W= Het1-,

R6NHC(O)- or COOP1

a35) Reacting a compound of the formula XXIIX alfabeta unsaturated ketone such as XLII, a so-called conjugate addition, to form a compound of the formula XLIII, in an inert solvent such as DCM using a base such as diethylamine.

a36) Reacting a compound of the formula XLIII to form a compound of the formula XLIV, using a fluorination reagens such as DAST in ann inerst solvent such as DCM.



[0106] The compounds of the present invention may be prepared by a process of preparing a compound of the formula XLVI comprising step a37

a37) Reacting a compound of formula XLV to form a compound of formula XLVI, using a lewis acid such as BF3·OEt2 in an inert solvent such as CHCl3.

[0107] The compounds of the present invention may be prepared by a process of preparing a compound of the formula XLVIII comprising step a38

a38) Reacting a compound of formula XLVII with ammonium chloride to form a compound of formula XLVIII, using a reagent such as HATU, a base such as TEA in an inert solvent such as DMF.

[0108] The compounds of the present invention may be prepared by a process of preparing a compound of the formula XLIX comprising step a39

a39) Reacting a compound of formula XLIX with hydrogen gas to form a compound of formula L, using a reagent such as Pd/C in an inert solvent such as methanol.

[0109] The compounds of the present invention may be prepared by a process of preparing a compound of the formula LIII comprising steps a40-a41

a40) Reacting a compound of formula LI with hydrogen gas to form a compound of formula LII, using a reagent such as Pd/C in an inert solvent such as methanol. Optionally with the addition of DCM. An alternative approach would be to react LI with triphenylphosphine to form LII in an inert solvent such as DCM.

a41) Reacting a compound of formula LII with a compound of the formula R13-COOH to give a compound of formula LIII, using a coupling reagent such as HATU in an inert solvent such as DMF. Optionally with the addition of an organic base such as diisopropylethylamine.



[0110] The compounds of the present invention may be prepared by a process of preparing a compound of the formula LVII comprising step a42-a44

a42) Reacting a compound of the formula LIV with ethyl propiolate to give a compound of formula LV using CuI in an inert solvent such as DMF or acetonitrile, using a base, such as diisopropylethylamine.

a43) Reacting a compound of formula LV with a base such as potassium hydroxide to give a compound of formula LVI.

a44) Reacting a compound of formula LVI with a ccompound of formula R6-NH2 Using a coupling reagent such as HATU in an inert solvent such as DMF. Optionally in the presence of an organic base such as diisopropylethyl amine.



[0111] The compounds of the present invention may be prepared by a process of preparing a compound of the formula LIX comprising step a45-a47

a45) Reacting a compound of formula LI with a compound of formula R27-CHOH-CC-H to give a compound of formula LVIII, using CuI in an inert solvent such as DMF or acetonitrile, using a base, such as diisopropylethylamine.

a46) Reacting a compound of formula LVIII with an oxidizing reagent such as Dess-Martin periodinane in an inert solvent such as DCM to give a compound of formula LIX.



[0112] The compounds of the present invention may be prepared by a process of preparing a compound of the formula LXI comprising step a47.

a47) Reacting a compound of the formula LX with a reducing agent such as SnCl2, in an inert solvent such as EtOAc to give a compound of formula LXI.

[0113] The compounds of the present invention may be prepared by a process of preparing a compound of the formula LXIII comprising step a48

a48) Reacting a compound of the formula LXII with Na2S●10H2O in the presence of a base such as NaOH in an inert solvent such as DMF to give a compound of formula LXIII.

[0114] The compounds of the present invention may be prepared by a process of preparing a compound of the formula LXV comprising step a49

a49) Reacting a compound of the formula LXIV with triethylamine in an in methanol and water to give a compound of formula LXV

[0115] The compounds of the present invention may be prepared by a process of preparing a compound of the formula LXVII comprising step a50

a50) Reacting a compound of formula LXVI with with a reducing agent such as triphenylphosphine in an inert solvent such as toluene to give a compound of formula LXIII

[0116] The compounds of the present invention may be prepared by a process of preparing a compound of the formula LXVII comprising step a51

a51) Reacting a compound of formula LXVII with with a reducing agent such as triphenylphosphine in an inert solvent such as toluene to give a compound of formula LXVIII

[0117] The compounds of the present invention may be prepared by a process of preparing a compound of the formula LXX comprising step a52

a52) Reacting a compound of formula LXIX with methyl iodide using a base such as potassium carbonate in an inert solvent such as acetone to give a compound of formula LXX.

[0118] The compounds of the present invention may be prepared by a process of preparing a compound of the formula XXXVI comprising step a53.

a53) Reacting a compound of formula LXXI with a reducing agent such as Fe, in the presence of ammonium chloride in a solvent mixture of ethenaol and water to give a compound of formula XXXVI.

[0119] The compounds of the present invention may be prepared by a process of preparing a compound of the formula LXXIV comprising step a54-a55

a54) Reacting a compound of formula XXII with sodium iodide, in the presence of trimethylsilyl chloride in an inert solvent such as acetonitrile to give a compound of formula LXXIII.

a55) Reacting a compound of formula LXXII with trimethyl(trifluoromethyl)silane in a solvent such as NMP the presence of a preheated mixture of CuI and potassium fluoride to give a compound of formula LXXIV.


Detailed Description of the invention



[0120] The present compounds of formula (1) differ from prior art compounds in particular in that the pyranose ring is α-D-galactopyranose. It is important to emphasize that alpha and beta anomers are very different isomers and it is by no means considered to be obvious to the skilled person to expect same or similar activity of both anomers. Consequently alpha and beta anomers do not in general posses the same activity, and this is common knowledge to the skilled person. In general the compounds of formula (1) are >10 fold better with respect to galectin-3 affinity compared to the corresponding beta-anomers.

[0121] In a broad aspect the present invention relates to a D-galactopyranose compound of formula (1)

wherein
the pyranose ring is α-D-galactopyranose,
A is selected from

wherein Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br; F; Cl; CN; NR19R20, wherein R19 and R20 are independently selected from H, C1-3 alkyl, cyclopropyl, iso-propyl, - C(=O)-R21, wherein R21 is selected from H and C1-3 alkyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; iso-propyl, optionally substituted with a F; O-cyclopropyl optionally substituted with a F; O-isopropyl optionally substituted with a F; and OC1-3 alkyl optionally substituted with a F;

wherein R1-R5 are independently selected from H, CN, NH2, F, methyl optionally substituted with a fluorine (F), and OCH3 optionally substituted with a F;

wherein R6 is selected from C1-6 alkyl optionally substituted with a halogen, branched C3-6 alkyl and C3-7 cycloalkyl;

X is selected from S, SO, SO2, O, C=O, and CR7R8 wherein R7 and R8 are independently selected from hydrogen, OH, or halogen (e.g. F, Cl, Br);

wherein R27 is selected from a C1-6 alkyl, branched C3-6 alkyl, C1-6 alkoxy and branched C3-6 alkoxy;

B is selected from a) a C1-6 alkyl or branched C3-6 alkyl substituted with a five or six membered heteroaromatic ring, optionally substituted with a substituent selected from CN, a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R14-CONH- wherein R14 is selected from C1-3 alkyl and cyclopropyl; or a C1-6 alkyl substituted with a phenyl, optionally substituted with a substituent selected from CN, a halogen (e.g. Cl, F, Br, I), methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R15-CONH- wherein R15 is selected from C1-3 alkyl and cyclopropyl; b) an aryl, such as phenyl or naphthyl, optionally substituted with a group selected from a halogen (e.g. Cl, F, Br, I); CN; -COOH; -CONR22R23, wherein R22 and R23 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; NR28R29, wherein R28 and R29 are independently selected from H, C1-3 alkyl and isopropyl; OH; and R16-CONH- wherein R16 is selected from C1-3 alkyl and cyclopropyl; c) a C5-7 cycloalkyl, optionally substituted with a substituent selected from a halogen (e.g. Cl, F, Br, I), CN, methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R17-CONH- wherein R17 is selected from C1-3 alkyl and cyclopropyl; and d) a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with a group selected from a halogen (e.g. Cl, F, Br, I); CN; -COOH; -CONR24R25, wherein R24 and R25 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; NR30R31, wherein R30 and R31 are independently selected from H, C1-3 alkyl and isopropyl; OH; and R18-CONH- wherein R18 is selected from C1-3 alkyl and cyclopropyl; e) a C1-6 alkyl or branched C3-6 alkyl; or a pharmaceutically acceptable salt or solvate thereof.



[0122] In one particular embodiment the compounds of formula (1) are 15-80 fold better with respect to galectin-3 affinity compared to the corresponding beta-anomers, wherein
A is selected from formula (2) wherein R1-R5 are independently selected from H and F, provided that at least one of R1-R5 is F, typically 2 or 3 F;
X is selected from S, SO or SO2; and
B is selected from
  1. i) phenyl substituted with one, two or three groups selected from halogen; CN; -COOH; -CONR22R23, wherein R22 and R23 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; NR28R29, wherein R28 and R29 are independently selected from H, C1-3 alkyl and isopropyl; OH; and R16-CONH-wherein R16 is selected from C1-3 alkyl and cyclopropyl; provided that the 3-position relative to X is substituted with one atom selected from Cl, Br and I;
  2. ii) pyridin substituted with one, two or three groups selected from halogen; CN; -COOH; -CONR22R23, wherein R22 and R23 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; NR28R29, wherein R28 and R29 are independently selected from H, C1-3 alkyl and isopropyl; OH; and R16-CONH-wherein R16 is selected from C1-3 alkyl and cyclopropyl; provided that the 3-position relative to X is substituted with one atom selected from Cl, Br and I;
  3. iii) pyridazin substituted with one, two or three groups selected from halogen; CN; -COOH; -CONR22R23, wherein R22 and R23 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; NR28R29, wherein R28 and R29 are independently selected from H, C1-3 alkyl and isopropyl; OH; and R16-CONH-wherein R16 is selected from C1-3 alkyl and cyclopropyl; provided that the 3-position relative to X is substituted with one atom selected from Cl, Br and I.


[0123] In an embodiment A is selected from formula (2) wherein R1-R5 are independently selected from H, CN, NH2, F, methyl optionally substituted with a fluorine (F), and OCH3 optionally substituted with a F. In a further embodiment R1 is selected from H, CN, NH2, F, methyl optionally substituted with a fluorine (F), and OCH3 optionally substituted with a F, such as H, CN, NH2, F, methyl, CF3, OCH3 and OCF3. In a further embodiment R1 is selected from H and F. In a further embodiment R2 is selected from H, CN, NH2, F, methyl optionally substituted with a fluorine (F), and OCH3 optionally substituted with a F, such as H, CN, NH2, F, methyl, CF3, OCH3 and OCF3. In a further embodiment R2 is selected from F. In a further embodiment R3 is selected from H, CN, NH2, F, methyl optionally substituted with a fluorine (F), and OCH3 optionally substituted with a F, such as H, CN, NH2, F, methyl, CF3, OCH3 and OCF3. In a further embodiment R3 is selected from H, OCH3 and F. In a further embodiment R4 is selected from H, CN, NH2, F, methyl optionally substituted with a fluorine (F), and OCH3 optionally substituted with a F, such as H, CN, NH2, F, methyl, CF3, OCH3 and OCF3. In a further embodiment R4 is selected from H and F. In a further embodiment R5 is selected from H, CN, NH2, F, methyl optionally substituted with a fluorine (F), and OCH3 optionally substituted with a F, such as H, CN, NH2, F, methyl, CF3, OCH3 and OCF3. In a further embodiment R5 is selected from H and F.

[0124] In another embodiment A is selected from formula (3) wherein Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br; F; Cl; CN; NR19R20, wherein R19 and R20 are independently selected from H, C1-3 alkyl, cyclopropyl, iso-propyl, -C(=O)-R21, wherein R21 is selected from H and C1-3 alkyl; methyl optionally substituted with a F; and OCH3 optionally substituted with a F. In a further embodiment A is selected from formula (3) wherein Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F, and OCH3 optionally substituted with a F. In a further embodiment Het1 is selected from a five membered heteroaromatic ring. In a further embodiment Het1 is selected from a five membered heteroaromatic ring substituted with a group selected from Br, F, Cl, methyl, CF3, OCH3 and OCF3.

[0125] In a further embodiment Het1 is selected from a six membered heteroaromatic ring.

[0126] In a further embodiment Het1 is selected from a six membered heteroaromatic ring substituted with a group selected from Br, F, Cl, methyl, CF3, OCH3 and OCF3. In a further embodiment Het1 is selected from a six membered heteroaromatic ring substituted with a group selected from Br, F, and Cl.

[0127] In a further embodiment Het1 is selected from pyridinyl, pyrimidinyl, pyrazinyl, pyridazyl, oxazoyl, thiazoyl, thiadiazoly, oxadiazoyl, thiophenyl, and imidazolyl optionally substituted with a group selected from Br, F, Cl, methyl, CF3, OCH3 and OCF3. In a further embodiment Het1 is selected from pyridinyl substituted with a group selected from Br, F, and Cl. In a further embodiment Het1 is selected from pyridinyl substituted with a group selected from F, such as one, two or three F, typically 3 F.

[0128] In a further embodiment A is selected from formula (4) wherein R6 is selected from C1-6 alkyl, branched C3-6 alkyl and C3-7 cycloalkyl. In an embodiment R6 is a C1-6 alkyl, such as methyl or ethyl. In another embodiment R6 is a cyclopropyl, cyclopentyl or cyclohexyl.

[0129] In a further embodiment A is selected from formula (4) wherein R6 is selected from C1-6 alkyl substituted with a halogen. In a further embodiment R6 is a C1-3 alkyl substituted with one, two or three halogens, such as one, two or three F, e.g. CH2CF3.

[0130] In a further embodiment A is selected from formula (8) wherein R27 is selected from a C1-6 alkyl, branched C3-6 alkyl, C1-6 alkoxy and branched C3-6 alkoxy. In a further embodiment R27 is selected from a C1-6 alkyl, such as C1-4 alkyl, e.g. methyl, ethyl, propyl. In a further embodiment R27 is selected from a C1-6 alkoxy, such as C1-4 alkoxy, e.g. methoxy, ethoxy, propoxy.

[0131] In a still further embodiment X is selected from S, SO, SO2, O, C=O, and CR7R8 wherein R7 and R8 are independently selected from hydrogen, OH, or halogen (e.g. F, Cl, Br). In a further embodiment X is selected from S. In a further embodiment X is selected from SO. In a further embodiment X is selected from SO2. In a further embodiment X is selected from O. In a further embodiment X is selected from C=O. In a further embodiment X is selected from CR7R8 wherein R7 and R8 are independently selected from hydrogen, OH, F, Cl, and Br, such as CH2, CHF, CHCl, CHBr, CHOH, CF2, CCl2 and CBr2.

[0132] In a still further embodiment B is selected from a C1-6 alkyl or branched C3-6 alkyl substituted with a five or six membered heteroaromatic ring, optionally substituted with a substituent selected from a halogen (e.g. Cl, F, Br, I), CN, methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R14-CONH- wherein R14 is selected from C1-3 alkyl and cyclopropyl. In a further embodiment B is selected from a C1-6 alkyl substituted with a five membered heteroaromatic ring. In a further embodiment B is selected from a branched C3-6 alkyl substituted with a five membered heteroaromatic ring. In a further embodiment B is selected from a C1-6 alkyl substituted with a five membered heteroaromatic ring substituted with a substituent selected from CN, Cl, F, Br, I, methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R14-CONH- wherein R14 is selected from C1-3 alkyl and cyclopropyl. In a further embodiment B is selected from a C1-6 alkyl substituted with a five membered heteroaromatic ring substituted with a substituent selected from CN, Cl, F, Br, I, methyl, CF3, OCH3, OCF3, OCH2CH3, OCF2CF3, OH, and R14-CONH- wherein R14 is selected from methyl. In a further embodiment B is selected from a branched C3-6 alkyl substituted with a five membered heteroaromatic ring substituted with a substituent selected from CN, Cl, F, Br, I, methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R14-CONH- wherein R14 is selected from C1-3 alkyl and cyclopropyl. In a further embodiment B is selected from a branched C3-6 alkyl substituted with a five membered heteroaromatic ring substituted with a substituent, such as 1, 2 or 3, selected from CN, Cl, F, Br, I, methyl, CF3, OCH3, OCF3, OCH2CH3, OCF2CF3, OH, and R14-CONH- wherein R14 is selected from methyl. In a further embodiment B is selected from a C1-6 alkyl substituted with a phenyl, such as benzyl or CH2CH2-phenyl. In a further embodiment B is selected from a C1-6 alkyl substituted with a phenyl substituted with a substituent, such as 1, 2 or 3, selected from CN, Cl, F, Br, I, methyl, CF3, OCH3, OCF3, OCH2CH3, OCF2CF3, OH, and R15-CONH- wherein R15 is selected from methyl. In a further embodiment B is selected from a C1-6 alkyl substituted with a phenyl substituted with a group selected from Cl, F, Br, methyl, CF3, OCH3, OCF3, such as benzyl substituted with one or two Cl, or CH2CH2-phenyl substituted with one Cl. In a still further embodiment B is selected from a phenyl. In a further embodiment B is selected from a naphthyl. In a further embodiment B is selected from a naphthyl substituted with a group selected from NR28R29, wherein R28 and R29 are independently selected from H and C1-3 alkyl. In a further embodiment B is selected from a naphthyl substituted with a group selected from NR28R29, wherein R28 and R29 are independently selected from H and CH3. In a further embodiment B is selected from a naphthyl substituted with a group selected from NR28R29, wherein R28 and R29 selected from CH3. In a further embodiment B is selected from a phenyl substituted with a group, such as 1, 2 or 3, selected from Cl, F, Br, I, methyl, CF3, OCH3, OCF3, OCH2CH3, OCF2CF3, OH, and R16-CONH- wherein R16 is selected from methyl. In a still further embodiment B is selected from a phenyl substituted with a group, such as 1, 2 or 3, selected from Cl, F, Br, I, CN, methyl, CF3, OCH3, OCF3, OCH2CH3, OCF2CF3, OH, and R16-CONH-wherein R16 is selected from methyl. In a further embodiment B is selected from a phenyl substituted with a group, such as 1, 2 or 3, selected from COOH and CONH2. In a still further embodiment B is selected from a naphthyl substituted with a group, such as 1, 2 or 3, selected from Cl, F, Br, I, methyl, CF3, OCH3, OCF3, OCH2CH3, OCF2CF3, OH, and R16-CONH- wherein R16 is selected from methyl. In a further embodiment B is selected from a naphthyl. In a still further embodiment B is selected from a naphthyl substituted with a NR28R29, wherein R28 and R29 are independently selected from H, C1-3 alkyl and isopropyl, such as naphthyl substituted with a N(CH3)2.

[0133] In a further embodiment B is selected from a C5-7 cycloalkyl, such as cyclopentyl or cyclohexyl. In a further embodiment B is selected from cyclohexyl. In a still further embodiment B is selected from a C5-7 cycloalkyl, such as cyclohexyl, substituted with a group, such as 1, 2 or 3, selected from CN, Cl, F, Br, I, methyl, CF3, OCH3, OCF3, OCH2CH3, OCF2CF3, OH, and R17-CONH- wherein R17 is selected from methyl. In a still further embodiment B is selected from a cyclohexyl, substituted with a halogen, such as one or two halogens, typically F.

[0134] In a further embodiment of the present invention B is selected from a C1-4 alkyl. Typically, B is selected from ethyl, propyl and butyl.

[0135] In a still further embodiment B is selected from a heterocycle, such as heteroaryl or heterocycloalkyl. In a still further embodiment B is selected from a heteroaryl substituted with a group, such as 1, 2 or 3, selected from Cl, F, Br, I, methyl CF3, OCH3, OCF3, OCH2CH3, OCF2CF3, OH, and R18-CONH- wherein R18 is selected from methyl. In a further embodiment B is selected from a heteroaryl substituted with a group, such as 1, 2 or 3, selected from Cl, F, Br, I, CN, methyl, CF3, OCH3, OCF3, OCH2CH3, OCF2CF3, OH, and R18-CONH- wherein R18 is selected from methyl. In a still further embodiment B is selected from a heterocycloalkyl substituted with a group, such as 1, 2 or 3, selected from Cl, F, Br, I, methyl CF3, OCH3, OCF3, OCH2CH3, OCF2CF3, OH, and R18-CONH- wherein R18 is selected from methyl. In a further embodiment B is selected from a heteroaryl substituted with a group, such as 1, 2 or 3, selected from NR30R31, wherein R30 and R31 are independently selected from H, C1-3 alkyl and isopropyl. Typically, B is selected from a pyridinyl substituted with a group, such as 1, 2 or 3, selected from NH2, such as one NH2. In another embodiment B is selected from a thiophenyl substituted with a halogen, such as one Cl. In a further embodiment B is selected from a pyridazinyl. In a further embodiment B is selected from a pyridazinyl substituted with one, two or three, substituents seleted from OCH3, CN, OH and Cl.

[0136] In a further aspect the present invention relates to a compound of formula (1) of the present invention for use as a medicine.

[0137] In a still further aspect the present invention relates to a pharmaceutical composition comprising a compound of formula (1) of the present invention and optionally a pharmaceutically acceptable additive, such as a carrier and/or excipient.

[0138] In a further aspect the present invention relates to a compound of formula (1) of the present invention for use in a method for treating a disorder relating to the binding of a galectin-3 to a ligand in a mammal, such as a human. In an embodiment the disorder is selected from the group consisting of inflammation; fibrosis, such as pulmonary fibrosis, liver fibrosis, kidney fibrosis, ophthalmological fibrosis and fibrosis of the skin and heart; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancer, such as carcinomas, sarcomas, leukemias and lymphomas, such as T-cell lymphomas; metastasising cancers; autoimmune diseases, such as psoriasis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, ankylosing spondylitis, systemic lupus erythematosus; metabolic disorders; heart disease; heart failure; pathological angiogenesis, such as ocular angiogenesis or a disease or condition associated with ocular angiogenesis, e.g. neovascularization related to cancer; and eye diseases, such as age-related macular degeneration and corneal neovascularization; atherosclerosis; metabolic diseases such as diabetes; asthma and other interstitial lung diseases, including Hermansky-Pudlak syndrome, mesothelioma; liver disorders, such as non-alcoholic steatohepatitis. A non-limiting group of cancers given as examples of cancers that may be treated, managed and/or prevented by administration of a compound of formula (1) include: colon carcinoma, breast cancer, pancreatic cancer, ovarian cancer, prostate cancer, fibrosarcoma, myxosarcoma, liposarcoma, chondrosarcoma, osteogenic sarcoma, chordoma, angiosarcoma, endotheliosarcoma, lymphangeosarcoma, lymphangeoendothelia sarcoma, synovioma, mesothelioma, Ewing's sarcoma, leiomyosarcoma, rhabdomyosarcoma, squamous cell carcinoma, basal cell carcinoma, adenocarcinoma, sweat gland carcinoma, sebaceous gland carcinoma, papillary carcinoma, papillary adenocarcinomas, cystandeocarcinoma, medullary carcinoma, bronchogenic carcinoma, renal cell carcinoma, hepatoma, bile duct carcinoma, choriocarcinoma, seminoma, embryonal carcinoma, Wilms' tumor, cervical cancer, testicular tumor, lung carcinoma, small cell lung carcinoma, bladder carcinoma, epithelial carcinoma, glioblastomas, neuronomas, craniopharingiomas, schwannomas, glioma, astrocytoma, medulloblastoma, craniopharyngioma, ependymoma, pinealoma, hemangioblastoma, acoustic neuroama, oligodendroglioma, meningioma, melanoma, neuroblastoma, retinoblastoma, leukemias and lymphomas, acute lymphocytic leukemia and acute myelocytic polycythemia vera, multiple myeloma, Waldenstrom's macroglobulinemia, and heavy chain disease, acute nonlymphocytic leukemias, chronic lymphocytic leukemia, chronic myelogenous leukemia, Hodgkin's Disease, non-Hodgkin's lymphomas, rectum cancer, urinary cancers, uterine cancers, oral cancers, skin cancers, stomach cancer, brain tumors, liver cancer, laryngeal cancer, esophageal cancer, mammary tumors, childhood-null acute lymphoid leukemia (ALL), thymic ALL, B-cell ALL, acute myeloid leukemia, myelomonocytoid leukemia, acute megakaryocytoid leukemia, Burkitt's lymphoma, acute myeloid leukemia, chronic myeloid leukemia, and T cell leukemia, small and large non-small cell lung carcinoma, acute granulocytic leukemia, germ cell tumors, endometrial cancer, gastric cancer, cancer of the head and neck, chronic lymphoid leukemia, hairy cell leukemia and thyroid cancer. Each of these disorders is considered a single embodiment and may be made the subject of a claim specifically to such disease or disorder.

[0139] The skilled person will understand that it may be necessary to adjust or change the order of steps in the processes a1 to a55, and such change of order is encompassed by the aspects of the process as described above in the reaction schemes and accompanying description of the process steps.

[0140] Furthermore the skilled person will understand that the processes described above and hereinafter the functional groups of intermediate compounds may need to be protected by protecting groups.

[0141] Functional groups that it is desirable to protect include hydroxy, amino and carboxylic acid. Suitable protecting groups for hydroxy include optionally substituted and/or unsaturated alkyl groups (e.g. methyl, allyl, benzyl or tert-butyl), trialkyl silyl or diarylalkylsilyl groups (e.g. t-butyldimethylsilyl, t-butyldipheylsilyl or trimethylsilyl), AcO(acetoxy), TBS(t-butyldimethylsilyl), TMS(trimethylsilyl), PMB (p-methoxybensyl), and tetrahydropyranyl. Suitable proteting groups for carboxylic acid include (C1-6)-alkyl or benzyl esters. Suitable protecting groups for amino include t-butyloxycarbonyl, benzyloxycarbonyl, 2-(trimethylsilyl)-ethoxy-methyl or 2-trimethylsilylethoxycarbonyl (Teoc). Suitable protecting groups for S include S-C(=N)NH2, TIPS.

[0142] The protection and deprotection of functional groups may take place before or after any reaction in the above mentioned processes.

[0143] Furthermore the skilled person will appreciate, that, in order to obtain compounds of the invention in an alternative, and on some occasions more convenient manner, the individual process steps mentioned hereinbefore may be performed in different order, and/or the individual reactions may be performed at a different stage in the overall route (i.e. substituents may be added to and/or chemical transformations performed upon, different intermediates to those mentioned hereinbefore in conjunction with a particular reaction). This may negate, or render necessary, the need for protecting groups.

[0144] In a still further embodiment the compound (1) is on free form. "On free form" as used herein means a compound of formula (1), either an acid form or base form, or as a neutral compound, depending on the substitutents. The free form does not have any acid salt or base salt in addition. In one embodiment the free form is an anhydrate. In another embodiment the free form is a solvate, such as a hydrate.

[0145] In a further embodiment the compound (1) is a crystalline form. The skilled person may carry out tests in order to find polymorphs, and such polymorphs are intended to be encompassed by the term "crystalline form" as used herein.

[0146] When the compounds and pharmaceutical compositions herein disclosed are used for the above treatment, a therapeutically effective amount of at least one compound is administered to a mammal in need of said treatment.

[0147] The term "C1-x alkyl" as used herein means an alkyl group containing 1-x carbon atoms, e.g. C1-5 or C1-6, such as methyl, ethyl, propyl, butyl, pentyl or hexyl.

[0148] The term "branched C3-6 alkyl" as used herein means a branched alkyl group containing 3-6 carbon atoms, such as isopropyl, isobutyl, tert-butyl, isopentyl, 3-methylbutyl, 2,2-dimethylpropyl, n-hexyl, 2-methylpentyl, 2,2-dimethylbutyl, 2,3-dimethylbutyl.

[0149] The term "C3-7 cycloalkyl" as used herein means a cyclic alkyl group containing 3-7 carbon atoms, such as cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, and 1-methylcyclopropyl.

[0150] The term "C5-7 cycloalkyl" as used herein means a cyclic alkyl group containing 5-7 carbon atoms, such as cyclopentyl, cyclohexyl, or cycloheptyl.

[0151] The term "Oxo" as used herein means an oxygen atom with double bonds, also indicated as =O.

[0152] The term "CN" as used herein means a nitril.

[0153] The term "a five or six membered heteroaromatic ring" as used herein means one five membered heteroaromatic ring or one six membered heteroaromatic ring. The five membered heteroaromatic ring contains 5 ring atoms of which one to four are heteroatoms selected from N, O, and S. The six membered heteroaromatic ring contains 6 ring atoms of which one to five are heteroatoms selected from N, O and S. Examples include thiophene, furan, pyran, pyrrole, imidazole, pyrazole, isothiazole, isooxazole, pyridine, pyrazine, pyrimidine and pyridazine. When such heteroaromatic rings are substituents they are termed thiophenyl, furanyl, pyranyl, pyrrolyl, imidazolyl, pyrazolyl, isothiazolyl, isooxazolyl, pyridinyl, pyrazinyl, pyrimidinyl and pyridazinyl. Also included are oxazoyl, thiazoyl, thiadiazoly, oxadiazoyl, and pyridonyl.

[0154] The term "a heterocycle, such as heteroaryl or heterocycloalkyl" as used herein means a heterocycle consisting of one or more 3-7 membered ring systems containing one or more heteroatoms and wherein such ring systems may optionally be aromatic. The term "a heteroaryl" as used herein means a mono or bicyclic aromatic ringsystem containing one or more heteroatoms, such as 1-10, e.g. 1-6, selected from O, S, and N, including but not limited to oxazolyl, oxadiazolyl, thiophenyl, thiadiazolyl, thiazolyl, pyridyl, pyrimidinyl, pyridonyl, pyrimidonyl, quinolinyl, azaquionolyl, isoquinolinyl, azaisoquinolyl, quinazolinyl, azaquinazolinyl, bensozazoyl, azabensoxazoyl, bensothiazoyl, or azabensothiazoyl. The term "a heterocycloalkyl" as used herein means a mono or bicyclic 3-7 membered alifatic heterocycle containing one or more heteroatoms, such as 1-7, e.g. 1-5, selected from O, S, and N, including but not limited to piperidinyl, tetrahydropyranyl, tetrahydrothipyranyl, or piperidonyl.

[0155] The term "treatment" and "treating" as used herein means the management and care of a patient for the purpose of combating a condition, such as a disease or a disorder. The term is intended to include the full spectrum of treatments for a given condition from which the patient is suffering, such as administration of the active compound to alleviate the symptoms or complications, to delay the progression of the disease, disorder or condition, to alleviate or relief the symptoms and complications, and/or to cure or eliminate the disease, disorder or condition as well as to prevent the condition, wherein prevention is to be understood as the management and care of a patient for the purpose of combating the disease, condition, or disorder and includes the administration of the active compounds to prevent the onset of the symptoms or complications. The treatment may either be performed in an acute or in a chronic way. The patient to be treated is preferably a mammal; in particular a human being, but it may also include animals, such as dogs, cats, cows, sheep and pigs.

[0156] The term "a therapeutically effective amount" of a compound of formula (1) of the present invention as used herein means an amount sufficient to cure, alleviate or partially arrest the clinical manifestations of a given disease and its complications. An amount adequate to accomplish this is defined as "therapeutically effective amount". Effective amounts for each purpose will depend on the severity of the disease or injury as well as the weight and general state of the subject. It will be understood that determining an appropriate dosage may be achieved using routine experimentation, by constructing a matrix of values and testing different points in the matrix, which is all within the ordinary skills of a trained physician or veterinary.

[0157] In a still further aspect the present invention relates to a pharmaceutical composition comprising the compound of formula (1) and optionally a pharmaceutically acceptable additive, such as a carrier or an excipient.

[0158] As used herein "pharmaceutically acceptable additive" is intended without limitation to include carriers, excipients, diluents, adjuvant, colorings, aroma, preservatives etc. that the skilled person would consider using when formulating a compound of the present invention in order to make a pharmaceutical composition.

[0159] The adjuvants, diluents, excipients and/or carriers that may be used in the composition of the invention must be pharmaceutically acceptable in the sense of being compatible with the compound of formula (1) and the other ingredients of the pharmaceutical composition, and not deleterious to the recipient thereof. It is preferred that the compositions shall not contain any material that may cause an adverse reaction, such as an allergic reaction. The adjuvants, diluents, excipients and carriers that may be used in the pharmaceutical composition of the invention are well known to a person skilled within the art.

[0160] As mentioned above, the compositions and particularly pharmaceutical compositions as herein disclosed may, in addition to the compounds herein disclosed, further comprise at least one pharmaceutically acceptable adjuvant, diluent, excipient and/or carrier. In some embodiments, the pharmaceutical compositions comprise from 1 to 99 weight % of said at least one pharmaceutically acceptable adjuvant, diluent, excipient and/or carrier and from 1 to 99 weight % of a compound as herein disclosed. The combined amount of the active ingredient and of the pharmaceutically acceptable adjuvant, diluent, excipient and/or carrier may not constitute more than 100% by weight of the composition, particularly the pharmaceutical composition.

[0161] In some embodiments, only one compound as herein disclosed is used for the purposes discussed above.

[0162] In some embodiments, two or more of the compounds as herein disclosed are used in combination for the purposes discussed above.

[0163] The composition, particularly pharmaceutical composition comprising a compound set forth herein may be adapted for oral, intravenous, topical, intraperitoneal, nasal, buccal, sublingual, or subcutaneous administration, or for administration via the respiratory tract in the form of, for example, an aerosol or an air-suspended fine powder. Therefore, the pharmaceutical composition may be in the form of, for example, tablets, capsules, powders, nanoparticles, crystals, amorphous substances, solutions, transdermal patches or suppositories.

[0164] Further embodiments of the process are described in the experimental section herein, and each individual process as well as each starting material constitutes embodiments that may form part of embodiments.

[0165] The above embodiments should be seen as referring to any one of the aspects (such as 'method for treatment', 'pharmaceutical composition', 'compound for use as a medicament', or 'compound for use in a method') described herein as well as any one of the embodiments described herein unless it is specified that an embodiment relates to a certain aspect or aspects of the present invention.

[0166] All headings and sub-headings are used herein for convenience only and should not be construed as limiting the invention in any way.

[0167] Any combination of the above-described elements in all possible variations thereof is encompassed by the invention unless otherwise indicated herein or otherwise clearly contradicted by context.

[0168] The terms "a" and "an" and "the" and similar referents as used in the context of describing the invention are to be construed to cover both the singular and the plural, unless otherwise indicated herein or clearly contradicted by context.

[0169] Recitation of ranges of values herein are merely intended to serve as a shorthand method of referring individually to each separate value falling within the range, unless other-wise indicated herein, and each separate value is incorporated into the specification as if it were individually recited herein. Unless otherwise stated, all exact values provided herein are representative of corresponding approximate values (e.g., all exact exemplary values provided with respect to a particular factor or measurement can be considered to also pro-vide a corresponding approximate measurement, modified by "about," where appropriate).

[0170] All methods described herein can be performed in any suitable order unless otherwise indicated herein or otherwise clearly contradicted by context.

[0171] The use of any and all examples, or exemplary language (e.g., "such as") provided herein, is intended merely to better illuminate the invention and does not pose a limitation on the scope of the invention unless otherwise indicated. No language in the specification should be construed as indicating any element is essential to the practice of the invention unless as much is explicitly stated.

[0172] The citation and incorporation of patent documents herein is done for convenience only and does not reflect any view of the validity, patentability and/or enforceability of such patent documents.

[0173] The description herein of any aspect or embodiment of the invention using terms such as "comprising", "having", "including" or "containing" with reference to an element or elements is intended to provide support for a similar aspect or embodiment of the invention that "consists of', "consists essentially of', or "substantially comprises" that particular element or elements, unless otherwise stated or clearly contradicted by context (e.g., a composition described herein as comprising a particular element should be understood as also describing a composition consisting of that element, unless otherwise stated or clearly contradicted by context).

[0174] The present invention is further illustrated by the following examples that, however, are not to be construed as limiting the scope of protection. The features disclosed in the foregoing description and in the following examples may, both separately and in any combination thereof, be material for realizing the invention in diverse forms thereof.

Experimental procedures



[0175] 
ExampleNamestructureGalectin-3 Kd (µM)
1 3,4-Dimethylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.150
2 3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.037
3 3-Ethoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.420
4 4-Pyridyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.190
5 2,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.650
6 4-Acetanilidyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.313
7 4-Methoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.410
8 2,3-Dichlorophenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranoside

0.530
9 3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranoside

0.017
10 Benzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

1.130
11 3-Methoxyphenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.270
12 2-Naphtyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.180
13 3-Methylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.240
14 3-(Trifluoromethyl)-phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.130
15 4-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.520
16 3,5-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranoside

0.080
17 2,6-Dimethylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

1.580
18 1-Naphthyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.960
19 3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.049
20 3-(Trifluoromethoxy)phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.500
21 2-Pyridyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl-1H-1,2,3-triazol-1-yl]-)-1-thio-α-D-galactopyranoside

0.510
22 3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.49
23 4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.200
24 3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide

0.010
25 3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone

0.062
26 4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide

0.770
27 Phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.400
28 3-Chloro-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.048
29 4-Tolyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.460
30 4-Fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.270
31 4-Trifluoromethoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.280
32 Phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

1.000
33 3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.250
34 4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.570
35 Cyclohexyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.440
36 2,4,5-Trichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.060
37 2,5-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.084
38 3-Hydroxy-phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.490
39 3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide

0.023
40 2-Phenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.480
41 3,4-Dichlorophenyl 3-O-[(2-amino-(4-chlorophenyl) pyrimidin-6-yl)methylene]-1-thio-α-D-galactopyranoside

1.300
42 3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide

0.002
43 5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.035
44 5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.023
45 3-Chloro-5-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.041
46 3-Chloro-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.031
47 3-Chloro-6-fluoro-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.029
48 3-Bromo-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.043
49 5-Bromo-6-trifluoromethyl- pyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.023
50 5-Chloro-6-cyanopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.039
51 5-Chloro-6-cyanopyridazin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.054
52 3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.037
53 3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone

0.013
54 5-Methoxy-6-cyanopyridazin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.25
55 5-Hydroxy-6-cyanopyridazin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.94
56 3-Chloro-2,4-difluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.15
57 3,4-Dichlorophenyl 3-deoxy -3-[4-(phenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

1.45
58 3,5-Dichloro-4-fluorophenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl )-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.018
59 3,4-Dichloro-6-fluorophenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl )-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.027
60 3-Bromo-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.024
61 3-Chloro-4-(trifluoromethyl)phenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.031
62 3,4,5-Trichlorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.034
63 5-Chloro-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.046
64 5-Bromo-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.047
65 5-Chloro-2-methoxyphenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.084
66 3-Iodophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.090
67 Picolinamide-5-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.180
68 3-Cyanophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.192
69 2-Cyanopyridine-5-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.26
70 4-Chloro-2-thienyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.33
71 3-Carboxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.35
72 Benzamide-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.38
73 3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.11
74 3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranoside

0.11
75 3,4-Dichlorophenyl 3-deoxy-3-[4-(3-fluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.14
76 3,3'-difluoro-cyklohexyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranoside

0.24
77 n-Butyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.45
78 3,4-Dichlorophenyl 3-deoxy-3-[4-(3,5-difluoro-4-methoxyphenyl)-1H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranoside

0.47
79 2-Hydroxy-pyridin-4-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranoside

0.51
80 2-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranoside

0.75
81 4-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranoside

0.76
82 2-Chlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.77
83 3,4-Dichlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.79
84 3-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranoside

0.82
85 4-Chlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

0.95
86 Propyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.98
87 2-Aminopyridin-4-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranoside

0.99
88 5-dimethylaminonaphatlen-2-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

1.06
89 Ethyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside

1.1
90 5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide, isomer 1

0.028
91 5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide, isomer 2

0.130
92 5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone

0.031
93 5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide, isomer 1

0.106
94 5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide, isomer 2

0.041
95 5-Dimethylaminonaphtalen-2-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone

0.96
96 3,4-dichlorophenyl-3-deoxy-3-(3,4,5-trifluorobenzamido)- 1-thio-α-D-galactopyranoside

3.5
97 3,4-Dichlorophenyl 3-deoxy-3-[4-(ethoxycarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

3.5
98 3,4-Dichlorophenyl 3-deoxy-3-[4-(ethylaminocarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.56
99 3,4-Dichlorophenyl 3-O-[(5,6-Difluoro-2-oxo-3-chromenyl)methyl] -1-thio-α-D-galactopyranoside

0.92
100 5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.100
101 5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.047
102 3,4-Dichlorophenyl 3-deoxy-3-[4-(propylcarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

1.01
103 5-Chloro-6-trifluoromethyl- pyridine-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.026
104 5-Chloro-2-trifluoromethyl- pyridine-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1 H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.093
105 5-Chloro-6-cyanopyridine-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.074
106 3 -Chloro-4-cyanophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside

0.083

Evaluation of Kd values



[0176] The affinity of Example 1-106 for galectins were determined by a fluorescence anisotropy assay where the compound was used as an inhibitor of the interaction between galectin and a fluorescein tagged saccharide probe as described Sörme, P., Kahl-Knutsson, B., Huflejt, M., Nilsson, U. J., and Leffler H. (2004) Fluorescence polarization as an analytical tool to evaluate galectin-ligand interactions. Anal. Biochem. 334: 36-47, (Sörme et al., 2004) and Monovalent interactions of Galectin-1 By Salomonsson, Emma; Larumbe, Amaia; Tejler, Johan; Tullberg, Erik; Rydberg, Hanna; Sundin, Anders; Khabut, Areej; Frejd, Torbjorn; Lobsanov, Yuri D.; Rini, James M.; et al, From Biochemistry (2010), 49(44), 9518-9532, (Salomonsson et al., 2010). The assay was also adapted to be able to measure the high affinity of compounds for galectin-3 by using the below probe constructed to have high affinity for galectin-3 which made it possible to use a low concentration of galectin-3 (50 nM). 100 nM albumin was included as a carrier to prevent protein loss at such low concentration of galectin.


PK experiments



[0177] Good in vitro PK properties were observed for some of the compounds of Formula 1 which translated into high oral bioavailability, low clearance, good halflife and high Cmax at 10 mg/kg in pharmacokinetic studies in mice. This is examplified by Example 9 3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside in the table below.
 Clearance (CL) (ml/min/kg)Halflife (t1/2) (h)Bioavailability (F) (%)Cmax p. o. Dose 10 mg/kg (µM)
Example 9 1.2 4.5 75 16

Synthesis of Examples and intermediates


General procedures



[0178] Nuclear Magnetic Resonance (NMR) spectra were recorded on a 400 MHz Varian or a 500 MHz Bruker AVANCE III 500 instrument, at 25°C. Chemical shifts are reported in ppm (δ) using the residual solvent as internal standard. Peak multiplicities are expressed as follow: s, singlet; d, doublet; dd, doublet of doublets; t, triplet; dt, doublet of triplet; q, quartet; m, multiplet; br s, broad singlet.

[0179] LC-MS were acquired on an Agilent 1100 or Agilent 1200 HPLC coupled with an Agilent MSD mass spectrometer operating in ES (+) ionization mode. Columns: Waters symmetry 2.1 x 30 mm C18, Chromolith RP-18 2 x 50 mm or XBridge C18 (4.6 × 50 mm, 3.5 µm) or SunFire C18 (4.6 × 50 mm, 3.5 µm). Solvent A water + 0.1% TFA and solvent B Acetonitrile + 0.1% TFA. Wavelength: 254 nM

[0180] Preparative HPLC was performed on a Gilson system. A) Flow: 10 ml/min Column: kromasil 100-5-C18 column. Wavelength: 254 nM. Solvent A water + 0.1% TFA and solvent B Acetonitrile + 0.1% TFA. B) on a Gilson 215. Flow: 25 ml/min Column: XBrige prep C18 10 µm OBD (19 × 250 mm) column. Wavelength: 254 nM. Solvent A water (10 mM Ammonium hydrogen carbonate) and solvent B Acetonitrile.

[0181] Flash chromatography was perfomed on a Biotage SP1 automated system, using Biotage Snap KP-Sil 25 g or 50 g cartridges.

[0182] The following abbreviations are used
EtOAc:
Ethylacetate
DIEA:
N,N-Diisopropylethylamine
PE:
petroleum ether
NEt3:
Triethylamine
DMF:
N,N-dimethylformamide
BF3•OEt2:
Borontrifuoride etherate
ESI-MS:
Electrospray ionization mass spectrometry
Calcd:
Calculated
TFA:
trifluoroacetic acid
Rt:
Room temperature
UV:
Ultraviolet
PMA:
polymolybdenic acid
DAST:
Diethylaminosulfur trifluoride
TBDMS:
tert-Butyldimethylsilyl
TBAF:
tetrabutylammonium Fluoride
MTBE:
tert-butyl methyl ether
HATU:
1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate

Example 1


3,4-Dimethylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-1-thio-D-galactopyranoside



[0183] 



[0184] 3,4-Dimethyl-phenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside, i1, (46 mg, 0.10 mmol) was dissolved in anhydrous acetonitrile (3 mL) and stirred at rt under N2. CuI (20 mg, 0.11 mmol) was added and after five minutes 3,4,5-trifluorophenylacetylene (0.028 mL, 0.21 mmol) was added. After another five minutes DIEA (0.018 mL, 0.10 mmol) was added and the mixture was stirred at rt for eighteen hours. The mixture was filtered through a short silica column, eluting with EtOAc and concentrated down. The residue was dissolved in methanolic NaOMe (20 mL, 0.05 M) and stirred at rt. After four hours acetic acid (2 mL) was added and the mixture was concentrated down. The crude product was purified by preparative HPLC and lyophilized to give 28 mg (57%) of 3,4-dimethylphenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside. 1H NMR (400 MHz, Methanol-d4) δ 8.53 (s, 1H), 7.70 - 7.61 (m, 2H), 7.40 (s, 1H), 7.33 (d, J = 7.7 Hz, 1H), 7.10 (d, J = 7.7 Hz, 1H), 5.66 (d, J = 5.3 Hz, 1H), 4.98 (dd, J = 11.4, 2.7 Hz, 1H), 4.88 (dd, J = 11.6, 5.3 Hz, 1H), 4.59 (t, J = 6.1 Hz, 1H), 4.20 (s, 1H), 3.71 (qd, J = 11.4, 6.2 Hz, 2H), 2.26 (s, 6H). ESI-MS m/z calcd for [C22H23F3N3O4S]+ (M+H)+: 482.1; found: 482.1.

Example 2-16 were made from their corresponding intermediate i2-i16 using a similar prodedure as Example 1.


Example 2


3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0185] 



[0186] Yield 53%. 1H NMR (400 MHz, Methanol-d4) δ 8.54 (s, 1H), 7.80 (s, 1H), 7.71 - 7.55 (m, 3H), 7.45 (d, J = 8.5 Hz, 1H), 7.25 (t, J = 7.9 Hz, 1H), 5.83 (d, J = 5.1 Hz, 1H), 4.98 (dd, J = 11.5, 2.6 Hz, 1H), 4.91 (dd, J = 11.4, 5.2 Hz, 1H), 4.51 (t, J = 6.1 Hz, 1H), 4.21 (d, J = 1.6 Hz, 1H), 3.72 (qd, J = 11.4, 6.2 Hz, 2H). ESI-MS m/z calcd for [C20H18BrF3N3O4S]+ (M+H)+: 532.0; found: 531.9.

Example 3


3-Ethoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0187] 



[0188] Yield 78%. 1H NMR (400 MHz, Methanol-d4) δ 8.54 (s, 1H), 7.71 - 7.60 (m, 2H), 7.27 - 7.12 (m, 3H), 6.84 (d, J = 7.9 Hz, 1H), 5.78 (d, J = 5.2 Hz, 1H), 4.97 (dd, J = 11.5, 2.7 Hz, 1H), 4.89 (dd, J = 11.5, 5.3 Hz, 1H), 4.56 (t, J = 6.1 Hz, 1H), 4.21 (d, J = 1.7 Hz, 1H), 4.05 (q, J = 7.0 Hz, 2H), 3.72 (qd, J = 11.3, 6.2 Hz, 2H), 1.39 (t, J = 7.0 Hz, 3H). ESI-MS m/z calcd for [C22H23F3N3O5S]+ (M+H)+: 498.1; found: 498.0.

Example 4


4-Pyridyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0189] 



[0190] Yield 73%. 1H NMR (400 MHz, Methanol-d4) δ 8.58 (s, 1H), 8.52 (d, J = 6.8 Hz, 2H), 8.07 (d, J = 6.8 Hz, 2H), 7.71 - 7.58 (m, 2H), 6.49 (bs, 1H), 5.06 (d, J = 1.4 Hz, 2H), 4.25 (t, J = 6.0 Hz, 1H), 4.20 (s, 1H), 3.72 (dd, J = 5.9, 2.5 Hz, 2H). ESI-MS m/z calcd for [C19H18F3N4O4S]+ (M+H)+: 455.1; found: 455.0.

Example 5


2,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trinuorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0191] 



[0192] Yield 58%. 1H NMR (400 MHz, Methanol-d4) δ 8.55 (s, 1H), 7.77 (d, J = 8.5 Hz, 1H), 7.69 - 7.59 (m, 2H), 7.51 (d, J = 2.1 Hz, 1H), 7.32 (dd, J = 8.5, 2.1 Hz, 1H), 5.99 (d, J = 5.3 Hz, 1H), 5.07 (dd, J = 11.4, 2.7 Hz, 1H), 4.96 (dd, J = 11.4, 5.4 Hz, 1H), 4.44 (t, J = 6.2 Hz, 1H), 4.22 (d, J = 1.7 Hz, 1H), 3.70 (dd, J = 11.4, 5.9 Hz, 1H), 3.61 (dd, J = 11.4, 6.4 Hz, 1H). ESI-MS m/z calcd for [C20H17Cl2F3N3O4S]+ (M+H)+: 522.0; found: 522.0.

Example 6


4-Acetanilidyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0193] 



[0194] Yield 36%. 1H NMR (400 MHz, Methanol-d4) δ 8.53 (s, 1H), 7.64 (dd, J = 8.6, 6.8 Hz, 2H), 7.58 - 7.51 (m, 4H), 5.69 (d, J = 5.2 Hz, 1H), 4.98 (dd, J = 11.4, 2.7 Hz, 1H), 4.88 (dd, J = 11.5, 5.3 Hz, 1H), 4.58 (t, J = 6.1 Hz, 1H), 4.21 (d, J = 2.0 Hz, 1H), 3.72 (qd, J = 11.4, 6.2 Hz, 2H), 2.13 (s, 3H). ESI-MS m/z calcd for [C22H22F3N4O5S]+ (M+H)+: 511.1; found: 511.0.

Example 7


4-Methoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0195] 



[0196] Yield 49%. 1H NMR (400 MHz, Methanol-d4) δ 8.53 (s, 1H), 7.70 - 7.60 (m, 2H), 7.54 (d, J = 8.6 Hz, 2H), 6.91 (d, J = 8.7 Hz, 2H), 5.59 (d, J = 5.3 Hz, 1H), 4.98 (dd, J = 11.4, 2.8 Hz, 1H), 4.88 (d, J = 5.4 Hz, 1H), 4.61 (t, J = 6.1 Hz, 1H), 4.21 (d, J = 1.9 Hz, 1H), 3.80 (s, 3H), 3.72 (qd, J = 11.3, 6.1 Hz, 2H). ESI-MS m/z calcd for [C21H21F3N3O5S]+ (M+H)+: 484.1; found: 484.0.

Example 8


2,3-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0197] 



[0198] Yield 33%. 1H NMR (400 MHz, Methanol-d4) δ 8.55 (s, 1H), 7.75 (d, J = 8.0 Hz, 1H), 7.65 (dd, J = 8.4, 6.9 Hz, 2H), 7.43 (d, J = 8.0 Hz, 1H), 7.28 (t, J = 8.0 Hz, 1H), 6.05 (d, J = 5.4 Hz, 1H), 5.08 (dd, J = 11.4, 2.7 Hz, 1H), 4.98 (dd, J = 11.4, 5.3 Hz, 1H), 4.43 (t, J = 6.2 Hz, 1H), 4.22 (d, J = 2.2 Hz, 1H), 3.70 (dd, J = 11.4, 6.0 Hz, 1H), 3.61 (dd, J = 11.4, 6.3 Hz, 1H). ESI-MS m/z calcd for [C20H17Cl2F3N3O4S]+ (M+H)+: 522.0; found: 522.0.

Example 9


3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0199] 



[0200] Yield 77%. 1H NMR (400 MHz, Chloroform-d) δ 8.13 (s, 1H), 7.66 (d, J = 2.1 Hz, 1H), 7.48 - 7.35 (m, 4H), 5.86 (d, J = 5.3 Hz, 1H), 4.91 (dd, J = 11.0, 5.3 Hz, 1H), 4.73 (dd, J = 11.0, 2.5 Hz, 1H), 4.63 (s, 1H), 4.46 (t, J = 4.0 Hz, 1H), 4.14 - 4.00 (m, 2H). ESI-MS m/z calcd for [C20H16Cl2F3N3O4S]+ (M+H)+: 522.0; found: 521.9.

Example 10


Benzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0201] 



[0202] Yield 74%. 1H NMR (400 MHz, Chloroform-d) δ 7.95 (s, 1H), 7.31 - 7.09 (m, 7H), 5.45 (d, J = 5.1 Hz, 1H), 4.66 (dd, J = 11.0, 5.2 Hz, 1H), 4.59 (dd, J = 11.0, 2.5 Hz, 1H), 4.42 (d, J = 2.3 Hz, 1H), 4.24 (t, J = 4.4 Hz, 1H), 3.86 - 3.73 (m, 4H). ESI-MS m/z calcd for [C21H20F3N3O4S]+ (M+H)+: 468.1; found: 468.0.

Example 11


3-Methoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0203] 



[0204] Yield 69%. 1H NMR (400 MHz, Chloroform-d) δ 8.12 (s, 1H), 7.39 (dd, J = 8.1, 6.3 Hz, 2H), 7.30 - 7.23 (m, 1H), 7.16 - 7.07 (m, 2H), 6.92 - 6.84 (m, 1H), 5.87 (d, J = 5.2 Hz, 1H), 4.86 (dd, J = 11.1, 5.3 Hz, 1H), 4.73 (dd, J = 11.2, 2.4 Hz, 1H), 4.61 (d, J = 2.5 Hz, 1H), 4.51 (d, J = 4.0 Hz, 1H), 4.11 - 3.98 (m, 2H), 3.82 (s, 3H). ESI-MS m/z calcd for [C21H20F3N3O5S]+ (M+H)+: 484.1; found: 484.1.

Example 12


2-Naphtyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0205] 



[0206] Yield 56%. 1H NMR (400 MHz, Chloroform-d) δ 8.16 (s, 1H), 8.07 (s, 1H), 7.88 - 7.78 (m, 3H), 7.60 (d, J = 8.6 Hz, 1H), 7.57 - 7.50 (m, 2H), 7.47 (d, J = 7.1 Hz, 2H), 5.96 (d, J = 5.2 Hz, 1H), 4.90 (dd, J = 11.2, 5.2 Hz, 1H), 4.79 (d, J = 11.3 Hz, 1H), 4.65 (s, 1H), 4.58 (s, 1H), 4.14 - 4.01 (m, 2H). ESI-MS m/z calcd for [C24H20F3N3O4S]+ (M+H)+: 504.1; found: 504.0.

Example 13


3-Methylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0207] 



[0208] Yield 58%. 1H NMR (400 MHz, Chloroform-d) δ 8.11 (s, 1H), 7.43 - 7.31 (m, 4H), 7.24 (d, J = 7.6 Hz, 1H), 7.16 (d, J = 7.6 Hz, 1H), 5.83 (d, J = 5.1 Hz, 1H), 4.90 - 4.81 (m, 1H), 4.72 (dd, J = 11.1, 2.4 Hz, 1H), 4.61 (d, J = 1.9 Hz, 1H), 4.51 (t, J = 4.2 Hz, 1H), 4.08 - 4.01 (m, 2H), 2.36 (s, 3H). ESI-MS m/z calcd for [C21H20F3N3O4S]+ (M+H)+: 468.1; found: 468.0.

Example 14


3-(Trifluoromethyl)phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0209] 



[0210] Yield 65%. 1H NMR (400 MHz, Methanol-d4) δ 8.55 (d, J = 2.2 Hz, 1H), 7.92 - 7.84 (m, 2H), 7.64 (ddd, J = 9.0, 6.6, 2.4 Hz, 2H), 7.59 (d, J = 8.2 Hz, 1H), 7.53 (td, J = 7.7, 2.2 Hz, 1H), 5.88 (dd, J = 5.3, 2.3 Hz, 1H), 5.01 (dt, J = 11.6, 2.6 Hz, 1H), 4.98 - 4.89 (m, 1H), 4.58 - 4.50 (m, 1H), 4.23 (s, 1H), 3.72 (dddd, J = 26.3, 9.2, 6.2, 3.3 Hz, 2H). ESI-MS m/z calcd for [C21H17F6N3O4S]+ (M+H)+: 522.1; found: 522.0.

Example 15


4-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0211] 



[0212] Yield 0.2%. 1H NMR (400 MHz, Chloroform-d) δ 8.14 (s, 1H), 7.54 - 7.39 (m, 6H), 5.83 (d, J = 5.3 Hz, 1H), 4.88 (dd, J = 11.0, 5.2 Hz, 1H), 4.73 (dd, J = 11.0, 2.5 Hz, 1H), 4.63 (s, 1H), 4.49 (d, J = 4.1 Hz, 1H), 4.13 - 3.99 (m, 2H). ESI-MS m/z calcd for [C20H17BrF3N3O4S]+ (M+H)+: 532.0; found: 532.0.

Example 16


3,5-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranoside



[0213] 



[0214] Yield 14%. 1H NMR (400 MHz, Chloroform-d) δ 8.09 (s, 1H), 7.37 (d, J = 1.7 Hz, 2H), 7.32 - 7.20 (m, 3H), 5.85 (d, J = 5.2 Hz, 1H), 4.90 (dd, J = 11.2, 5.2 Hz, 1H), 4.70 (d, J = 11.2 Hz, 1H), 4.56 (s, 1H), 4.40 (s, 1H), 4.03 - 3.97 (m, 2H). ESI-MS m/z calcd for [C20H16Cl2F3N3O4S]+ (M+H)+: 522.0; found: 521.9.

Example 17


2,6-Dimethylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0215] 



[0216] 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (123 mg, 0.35 mmol) dissolved in anhydrous DMF (1 mL) and Cs2CO3 (184 mg, 0.56 mmol) was added. After five minutes 2,6-dimethylbenzenethiol (0.052 mL, 0.39 mmol) was added and the mixture was heated to 50 °C. After two hours the reaction was allowed to cool to rt and MeCN (2 mL) was added followed by CuI (34 mg, 0.18 mmol). After five minutes 3,4,5-trifluorophenylacetylene (0.095 mL, 0.71 mmol) was added and after an additional five minutes DIEA (0.092 mL, 0.53 mmol) was added and the mixture was stirred at rt for eighteen hours. The mixture was filtered through a short silica column, eluting with EtOAc and concentrated down. The residue was dissolved in methanolic NaOMe (10 mL, 0.05 M) and stirred at rt After four hours acetic acid (2 mL) was added and the mixture was concentrated down. The crude product was purified by preparative HPLC and lyophilized to give 24 mg (11%) of 2,6-dimethylphenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside. 1H NMR (400 MHz, Methanol-d4) δ 8.53 (s, 1H), 7.84 - 7.58 (m, 2H), 7.14 (bs, 3H), 5.36 (d, J = 5.3 Hz, 1H), 5.03 (dd, J = 11.6, 2.6 Hz, 1H), 4.83 - 4.76 (m, 1H), 4.56 (t, J = 6.2 Hz, 1H), 4.24 (d, J = 1.4 Hz, 1H), 3.78 - 3.57 (m, 2H), 2.60 (s, 6H). ESI-MS m/z calcd for [C22H23F3N3O4S]- (M+H)+: 482.1; found: 482.1.

Example 18-21 were made from their corresponding thiol using a similar procedure as Example 17;


Example 18


1-Naphthyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0217] 



[0218] Yield 17%. 1H NMR (400 MHz, Methanol-d4) δ 8.63 - 8.58 (m 1H), 8.56 (s, 1H), 7.99 - 7.82 (m, 3H), 7.76 - 7.50 (m, 4H), 7.47 (t, J = 7.7 Hz, 1H), 5.73 (d, J = 5.3 Hz, 1H), 5.13 (dd, J = 11.5, 2.8 Hz, 1H), 4.93 (dd, J = 11.5, 5.4 Hz, 1H), 4.69 (t, J = 6.2 Hz, 1H), 4.27 (d, J = 2.0 Hz, 1H), 3.76 - 3.69 (m, 1H), 3.63 (dd, J = 11.3, 6.3 Hz, 1H). ESI-MS m/z calcd for [C24H21F3N3O4S]+ (M+H)+: 504.1, found: 504.0.

Example 19


3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0219] 



[0220] Yield 25%. 1H NMR (400 MHz, Chloroform-d) δ 8.10 (s, 1H), 7.52 (d, J = 1.9 Hz, 1H), 7.40 (dt, J = 7.2, 1.7 Hz, 1H), 7.35 - 7.22 (m, 4H), 5.85 (d, J = 5.2 Hz, 1H), 4.90 (dd, J = 11.1, 5.3 Hz, 1H), 4.73 (dd, J = 11.1, 2.5 Hz, 1H), 4.59 (d, J = 2.5 Hz, 1H), 4.46 (t, J = 4.3 Hz, 1H), 4.06 - 3.90 (m, 2H). ESI-MS m/z calcd for [C20H17ClF3N3O4S]+ (M+H)+: 488.1; found: 488.0.

Example 20


3-(Trifluoromethoxy)phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0221] 



[0222] Yield 4%. 1H NMR (400 MHz, Chloroform-d) δ 8.15 (s, 1H), 7.55 - 7.36 (m, 5H), 7.21 (d, J = 8.7 Hz, 1H), 5.92 (d, J = 5.3 Hz, 1H), 4.91 (dd, J = 11.0, 5.1 Hz, 1H), 4.75 (q, J = 14.4, 14.0 Hz, 1H), 4.64 (s, 1H), 4.50 - 4.45 (m, 1H), 4.14 - 4.00 (m, 2H). ESI-MS m/z calcd for [C21H17F6N3O5S]+ (M+H)+: 538.1; found: 538.1.

Example 21


2-Pyridyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl-1H-1,2,3-triazol-1-yl]-)-1-thio-α-D-galactopyranoside



[0223] 



[0224] Yield 4%. 1H NMR (400 MHz, Methanol-d4) δ 8.57 (s, 1H), 8.50 (d, J = 5.3 Hz, 1H), 7.90 (dd, J = 8.9, 7.1 Hz, 1H), 7.78 (d, J = 7.7 Hz, 1H), 7.66 (t, J = 7.6 Hz, 2H), 7.38 (t, J = 7.4 Hz, 1H), 6.50 (s, 1H), 5.01 (t, J = 1.9 Hz, 2H), 4.35 (t, J = 6.2 Hz, 1H), 4.20 (s, 1H), 3.75 - 3.62 (m, 2H). ESI-MS m/z calcd for [C19H17F3N4O4S]+ (M+H)+: 455.1; found: 455.0.

Examples 22-23 were made from their corresponding intermediate i22-i23 using a similar prodedure as Example 1;


Example 22


1-O-(3,4-Dichlorophenyl) 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0225] 



[0226] Yield 42%. 1H NMR (400 MHz, Methanol-d4) δ 8.59 (s, 1H), 7.78 - 7.58 (m, 2H), 7.53 - 7.40 (m, 2H), 7.21 (dd, J = 8.9, 2.7 Hz, 1H), 5.69 (d, J = 3.6 Hz, 1H), 5.27 (dd, J = 11.4, 2.8 Hz, 1H), 4.69 (dd, J = 11.4, 3.6 Hz, 1H), 4.21 (d, J = 2.0 Hz, 1H), 4.11 (t, J = 6.1 Hz, 1H), 3.72 (d, J = 6.1 Hz, 2H). ESI-MS m/z calcd for [C20H17Cl2F3N3O5]+ (M+H)+: 506.0; found: 506.0.

Example 23


4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0227] 



[0228] Yield 31%. 1H NMR (400 MHz, Chloroform-d) δ 8.12 (s, 1H), 7.49 (d, J = 8.5 Hz, 2H), 7.41 (t, J = 7.6 Hz, 2H), 7.34 (d, J = 8.4 Hz, 2H), 7.27 (d, J = 0.7 Hz, 2H), 5.82 (d, J = 5.2 Hz, 1H), 4.88 (dd, J = 11.1, 5.2 Hz, 1H), 4.73 (dd, J = 11.1, 2.3 Hz, 1H), 4.62 (s, 1H), 4.48 (t, J = 4.4 Hz, 1H), 4.11 - 3.97 (m, 2H). ESI-MS m/z calcd for [C20H17ClF3N3O4S]+ (M+H)+: 488.1; found: 488.0.

[0229] From the purification of the title compound, in addition product 4-chlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside was isolated (yield 6%).

Example 24


3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide



[0230] 



[0231] 3,4-Dichlorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside (30 mg, 0.06 mmol) was dissolved in acetic acid (4 ml). Aqueous 27% hydrogen peroxide solution (0.20 ml) was added. The mixture was heated to 45 °C. After 2 h the mixture was diluted with water (2 ml) and purified on HPLC column. The title compound was isolated as lyophilized white powder (20 mg, 65%).
1H NMR (400 MHz, Chloroform-d) δ 8.14 (s, 1H), 7.86 (d, J = 1.9 Hz, 1H), 7.71 (d, J = 8.3 Hz, 1H), 7.62 - 7.55 (m, 1H), 7.44 (dd, J = 8.0, 6.4 Hz, 2H), 5.32 (d, J = 10.9 Hz, 1H), 5.08 (d, J = 4.7 Hz, 1H), 5.03 - 4.95 (m, 1H), 4.91 (s, 1H), 4.52 (t, J = 4.9 Hz, 1H), 4.10 (dd, J = 12.1, 5.4 Hz, 1H), 4.02 (dd, J = 12.0, 4.4 Hz, 1H). ESI-MS m/z calcd for [C20H16Cl2F3N3O5S]+ (M+H)+: 538.0; found: 537.9.

Example 25


3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone



[0232] 



[0233] From the purification described for Example 24 was also isolated the title compound (1 mg, 3%).
1H NMR (400 MHz, Methanol-d4) δ 8.54 (s, 1H), 8.16 (d, J = 2.0 Hz, 1H), 7.92 (dd, J = 8.5, 2.0 Hz, 1H), 7.80 (d, J = 8.5 Hz, 1H), 7.65 (dd, J = 8.5, 6.7 Hz, 2H), 5.74 (dd, J = 11.5, 2.7 Hz, 1H), 5.34 (d, J = 6.4 Hz, 1H), 5.13 (dd, J = 11.5, 6.4 Hz, 1H), 4.52 (t, J = 6.1 Hz, 1H), 4.28 (d, J = 2.6 Hz, 1H), 3.60 (qd, J = 11.6, 5.9 Hz, 2H). ESI-MS m/z calcd for [C20H16Cl2F3N3O6S]+ (M+H)+: 554.0; found: 554.0.

Example 26


4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide



[0234] 



[0235] 4-Chlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside (10 mg, 0.02 mmol) was dissolved in acetic acid (3 ml). Hydrogen peroxide (100 µl) was added. The mixture was stirred at 45 °C 1 h and then diluted with water and freezedried. The residue was dissolved in MeOH (10 ml) and 1M sodium methoxide in methanol (1 ml) was added. After one night at rt the mixture was concentrated in vacuo and the residue purified by HPLC. The product was isolated after freezedrying as a white fluffy powder (3 mg, 37%).
1H NMR (400 MHz, Methanol-d4) δ 8.54 (s, 1H), 7.91 - 7.81 (m, 2H), 7.69 - 7.59 (m, 4H), 5.52 (dd, J = 11.4, 2.8 Hz, 1H), 5.13 (dd, J = 11.3, 5.6 Hz, 1H), 4.92 (d, J = 5.5 Hz, 1H), 4.26 (d, J = 2.8 Hz, 1H), 4.08 (t, J = 6.1 Hz, 1H), 3.53 (dd, J = 11.4, 5.8 Hz, 1H), 3.41 (dd, J = 11.4, 6.4 Hz, 1H). ESI-MS m/z calcd for [C20H17ClF3N3O5S]+ (M+H)+: 504.1; found: 504.0.

Example 27


Phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0236] 



[0237] To a solution of phenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (160 mg, α-anomer: β-anomer = 100:8, 0.38 mmol) in DMF (3 mL) were added trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (4) (170 mg, 0.74 mmol), copper(I) iodide (20 mg, 0.11 mmol) and triethylamine (0.26 mL, 1.85 mmol). The mixture was stirred at 100 °C for 1 h. Water (10 mL) was added and the mixture was filtrated. The filtrate was dilluted by DCM (40 mL), washed with brine. The organic layers were dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was dissolved in methanolic NaOMe (8 mL, pH = 9-10) and stirred at r.t for 2 h. Neutralization with H+ resin, filtered and concentrated to afford a syrup. The crude product was purified by prep-HPLC and then chiral-HPLC to afford the α-anomer product 40 mg as a white solid.
Chiral-HPLC conditions: instrument: SFC-80 (Thar, Waters), column: IC 20*250 mm,5 um (Decial), column temperature: 35 °C, mobile phase: CO2/ Methanol(0.1%NH4OH) = 65/35, flow rate: 80 g/min, back pressure: 100 bar, detection wavelength: 214 nm, cycle time: 4.2 min, sample solution: 60 mg dissolved in 15 mL methanol, injection volume: 5.0 mL.
1H NMR (500 MHz, Methanol-d4) δ 8.57 (s, 1H), 7.67 (d, J = 6.5 Hz, 1H), 7.65 (d, J = 7.0 Hz, 1H), 7.63-7.61 (m, 2H), 7.36-7.30 (m, 3H), 5.77 (d, J = 5.5 Hz, 1H), 5.00 (dd, J = 11.5, 2.5 Hz, 1H), 4.91 (d, J = 5.0 Hz, 1H), 4.57 (t, J = 6.5 Hz, 1H), 4.21 (d, J = 1.5 Hz, 1H), 3.74 (dd, J = 11.5, 6.0 Hz, 1H), 3.67 (dd, J = 11.5, 6.5 Hz, 1H). ESI-MS m/z calcd for [C20H19F3N3O4S]+ (M+H)+: 454.1; found: 454.0.

Example 28


3-Chloro-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0238] 



[0239] To a solution of 3-chloro-4-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (210 mg, 0.44 mmol) in DMF (3 mL) were added trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (200 mg, 0.88 mmol), copper(I) iodide (30 mg, 0.13 mmol) and triethylamine (0.30 mL, 2.2 mmol). The mixture was stirred at 100 °C for 1 h. Water (10 mL) was added and the mixture was filtrated. The filtrate was dilluted with DCM (40 mL), washed with brine. The organic layers were dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was dissolved in methanolic NaOMe (8 mL, pH = 9-10) and stirred at r.t for 2 h. Neutralization with H+ resin, filtered and concentrated to afford a syrup. The crude product was purified by prep-HPLC to afford the α-anomer product 120 mg as a white solid.
1H NMR (500 MHz, Methanol-d4) δ 8.57 (s, 1H), 7.80 (dd, J = 7.5, 2.5 Hz, 1H), 7.68 (dd, J = 9.0, 6.5 Hz, 2H), 7.60 (m, 1H), 7.25 (t, J = 9.0 Hz, 1H), 5.78 (d, J = 5.5 Hz, 1H), 5.00 (dd, J = 11.5, 3.0 Hz, 1H), 4.93 (t, J = 5.5 Hz, 1H), 4.55 (t, J = 6.0 Hz, 1H), 4.22 (d, J = 1.5 Hz, 1H), 3.78-3.70 (m, 2H). ESI-MS m/z calcd for [C20H17ClF4N3O4S]+ (M+H)+: 506.1; found: 506.0.

Example 29


4-Tolyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0240] 



[0241] To a solution of 4-tolyl-2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside (85 mg, 0.14 mmol) in MeOH (5 mL), methoxysodium solution (30%, 7.7 mg, 0.04 mmol) was added and the solution was stirred at room temperature for 1 h. TLC (silica gel, PE:EtOAc = 1:1) analysis indicated the total consumption of the starting material. After completion, the Dowex 50W×8 hydrogen form (100-200 mesh) exchange resin was added (pH = 7) and the mixture was filtered. The filtrate was concentrated and purified by prep-HPLC, lyophilized to afford product 14 mg as a white solid.
1H NMR (500 MHz, DMSO-d6) δ 8.79 (s, 1H), 7.87 - 7.83 (m, 2H), 7.45 (d, J= 8.0 Hz, 2H), 7.17 (d, J = 8.0 Hz, 2H), 5.77 (brs, 1H), 5.67 (d, J = 5.5 Hz, 1H), 5.50 (brs, 1H), 4.79 (dd, J = 11.5Hz, 3.0Hz, 1H), 4.73 - 4.70 (m, 2H), 4.34 (t, J = 6.0 Hz, 1H), 4.03 (s, 1H), 3.54 (brs, 1H), 3.42 - 3.38 (m, 1H), 2.30 (s, 3H). ESI-MS m/z calcd for [C21H20F3N3O4S]+ (M+H)]+: 468.1; found: 468.1.

Example 30


4-Fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0242] 



[0243] To a solution of 4-fluorophenyl-2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside (150 mg, 0.25 mmol) in MeOH (5 mL). Sodium methoxide solution (30%, 9 mg, 0.05 mmol) was added and the solution was stirred at room temperature for 1 h. TLC (silica gel, PE:EtOAc = 3:1, UV, PMA) analysis indicated the total consumption of the starting material. The Dowex 50W×8 hydrogen form (100-200 mesh) exchange resin was added (pH = 7) and the mixture was filtered. Removal of solvent gave a residue. The crude was purified by flash column chromatography (silica gel, DCM:MeOH = 20:1) to afford product 58 mg as a white solid.
1H NMR (500 MHz, DMSO-d6) δ 8.80 (s, 1H), 7.86 - 7.83 (m, 2H), 7.63 - 7.60 (m, 2H), 7.23-7.19 (m, 2H), 5.83 (s, 1H), 5.71 (d, J = 5.0 Hz, 1H), 5.51 (d, J = 6.0 Hz, 1H), 4.80 (dd, J = 11.5 Hz, 2.5 Hz, 1H), 4.75 - 4.71 (m, 2H), 4.33 (t, J = 6.0 Hz, 1H), 4.04 (s, 1H), 3.57 - 3.52 (m, 1H), 3.43 - 3.39 (m, 1H). ESI-MS m/z calcd for [C20H17F4N3O4S]+ (M+H)+: 472.1; found: 472.0.

Example 31


4-Trifluoromethoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0244] 



[0245] To a solution of 4-trifluoromethoxy (2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (150 mg, 0.23 mmol) in MeOH (5 mL), methoxysodium solution (30%, 8 mg, 0.05 mmol) was added and the solution was stirred at room temperature for another 1 h. TLC (silica gel, PE:EtOAc=3:1, UV, PMA) analysis indicated the total consumption of the starting material. The Dowex 50W×8 hydrogen form (100-200 mesh) exchange resin was added (pH = 7) and mixture was filtered. Removal of solvent gave a residue. The residue was purified by flash column chromatography (silica gel, DCM:MeOH = 20:1) to afford product 44 mg as a white solid.
1H NMR (500 MHz, DMSO-d6) δ 8.67 (s, 1H), 7.73 - 7.69 (m, 2H), 7.54 (d, J = 9.0 Hz, 2H), 7.21 (d, J = 8.0 Hz, 2H), 5.71 (dd, J = 8.5 Hz, 4.5Hz, 2H), 5.38 (d, J = 6.0 Hz, 1H), 4.67 (dd, J = 11.5 Hz, 2.5Hz, 1H), 4.62-4.60 (m, 2H), 4.15 (t, J = 6.5 Hz, 1H), 3.90 (dd, J = 6.0 Hz, 2.5Hz, 1H), 3.43 - 3.38 (m, 1H), 3.25 - 3.30 (m, 1H). ESI-MS m/z calcd for [C21H17F6N3O5S]+ (M+H)+: 538.1; found: 538.1.

Examples 32-38 were made using a similar procedure as Example 1 from the corresponding intermediate i32-i38


Example 32


Phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0246] 



[0247] Yield 70%. 1H NMR (400 MHz, Methanol-d4) δ 8.59 (s, 1H), 7.66 (dd, J = 8.6, 6.8 Hz, 2H), 7.32 (t, J = 7.9 Hz, 2H), 7.24 (d, J = 7.9 Hz, 2H), 7.05 (t, J = 7.2 Hz, 1H), 5.67 (d, J = 3.6 Hz, 1H), 5.31 (dd, J = 11.4, 2.8 Hz, 1H), 4.68 (dd, J = 11.4, 3.6 Hz, 1H), 4.23 (d, J = 2.4 Hz, 1H), 4.18 (t, J = 6.2 Hz, 1H), 3.72 (dd, J = 6.1, 3.4 Hz, 2H). ESI-MS m/z calcd for [C20H19F3N3O5]+ (M+H)+: 438.1; found: 438.1.

Example 33


3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0248] 



[0249] Yield 75%. 1H NMR (400 MHz, Methanol-d4) δ 8.59 (s, 1H), 7.70 - 7.63 (m, 2H), 7.34 - 7.27 (m, 2H), 7.20 (d, J = 8.5 Hz, 1H), 7.07 (d, J = 7.2 Hz, 1H), 5.69 (d, J = 3.6 Hz, 1H), 5.29 (dd, J = 11.5, 2.8 Hz, 1H), 4.69 (dd, J = 11.4, 3.6 Hz, 1H), 4.23 - 4.20 (m, 1H), 4.14 (t, J = 6.1 Hz, 1H), 3.72 (d, J = 6.0 Hz, 2H). ESI-MS m/z calcd for [C20H18ClF3N3O5]+ (M+H)+: 472.1; found: 472.0.

Example 34


3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0250] 



[0251] Yield 60%. 1H NMR (400 MHz, Methanol-d4) δ 8.59 (s, 1H), 7.69 - 7.62 (m, 2H), 7.31 (d, J = 9.0 Hz, 2H), 7.23 (d, J = 9.0 Hz, 2H), 5.65 (d, J = 3.4 Hz, 1H), 5.29 (dd, J = 11.4, 2.6 Hz, 1H), 4.68 (dd, J = 11.4, 3.5 Hz, 1H), 4.22 (d, J = 1.3 Hz, 1H), 4.14 (t, J = 6.0 Hz, 1H), 3.72 (d, J = 6.1 Hz, 2H). ESI-MS m/z calcd for [C20H18ClF3N3O5]+ (M+H)+: 472.1; found: 472.0.

Example 35


Cyclohexyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0252] 



[0253] Yield 99%. 1H NMR (400 MHz, Methanol-d4) δ 8.50 (s, 1H), 7.67 - 7.60 (m, 2H), 5.65 (d, J = 5.4 Hz, 1H), 4.91 (dd, J = 11.4, 2.8 Hz, 1H), 4.79 (dd, J = 11.4, 5.4 Hz, 1H), 4.43 (t, J = 6.1 Hz, 1H), 4.13 (s, 1H), 3.73 (d, J = 6.0 Hz, 2H), 2.96 (t, J = 10.0 Hz, 1H), 2.16 - 1.26 (m, 10H). ESI-MS m/z calcd for [C20H25F3N3O4S]+ (M+H)+: 460.2; found: 460.0.

Example 36


2,4,5-Trichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0254] 



[0255] Yield 65%. 1H NMR (400 MHz, Methanol-d4) δ 8.55 (s, 1H), 7.96 (s, 1H), 7.70 - 7.61 (m, 3H), 6.03 (d, J = 5.3 Hz, 1H), 5.06 (dd, J = 11.4, 2.3 Hz, 1H), 4.98 (dd, J = 11.4, 5.3 Hz, 1H), 4.40 (t, J = 6.0 Hz, 1H), 4.22 (s, 1H), 3.71 (dd, J = 11.3, 5.7 Hz, 1H), 3.64 (dd, J = 11.4, 6.5 Hz, 1H). ESI-MS m/z calcd for [C20H16Cl3F3N3O4S]+ (M+H)+: 556.0; found: 555.9.

Example 37


2,5-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0256] 



[0257] Yield 32%. 1H NMR (400 MHz, Methanol-d4) δ 8.55 (s, 1H), 7.81 (q, J = 1.7 Hz, 1H), 7.69 - 7.60 (m, 2H), 7.42 (dt, J = 8.5, 1.5 Hz, 1H), 7.25 (ddt, J = 8.7, 2.8, 1.5 Hz, 1H), 6.03 (d, J = 5.3 Hz, 1H), 5.11 - 5.02 (m, 1H), 4.98 (ddt, J = 11.5, 5.4, 1.5 Hz, 1H), 4.47 - 4.39 (m, 1H), 4.23 (q, J = 1.5 Hz, 1H), 3.73 (ddt, J = 11.4, 6.1, 1.5 Hz, 1H), 3.63 (ddt, J = 11.3, 6.4, 1.5 Hz, 1H). ESI-MS m/z calcd for [C20H17Cl2F3N3O4S]+ (M+H)+: 522.0; found: 522.1.

Example 38


3-Hydroxy-phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0258] 



[0259] Yield 14%. 1H NMR (400 MHz, Methanol-d4) δ 8.53 (s, 1H), 7.65 (dd, J = 8.6, 6.6 Hz, 2H), 7.14 (t, J = 7.9 Hz, 1H), 7.09 - 7.02 (m, 2H), 6.71 (dt, J = 7.9, 1.6 Hz, 1H), 5.78 (d, J = 5.2 Hz, 1H), 4.97 (dd, J = 11.5, 2.7 Hz, 1H), 4.94 - 4.87 (m, 1H), 4.54 (t, J = 6.3 Hz, 1H), 4.21 (d, J = 2.7 Hz, 1H), 3.73 (qd, J = 11.3, 6.1 Hz, 2H). ESI-MS m/z calcd for [C20H18F3N3O5S]+ (M+H)+: 470.1 ; found: 470.2.

Example 39


3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide



[0260] 



[0261] 3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (12 mg, 0.023 mmol) was dissolved in CH2Cl2 (3 mL) and stirred at rt. mCPBA (6.4 mg, 0.027 mmol) dissolved in CH2Cl2 (0.355 mL) was added. After 3 h the mixture was filter through a short silica column, eluting with EtOAc, and concentrated down. Purified on preparative HPLC and lyophilized to give 11 mg (89%) of the sulfoxide.
1H NMR (400 MHz, Methanol-d4) δ 8.54 (s, 1H), 8.03 (s, 1H), 7.78 (dd, J = 21.7, 7.9 Hz, 2H), 7.70 - 7.61 (m, 2H), 7.52 (t, J = 7.9 Hz, 1H), 5.55 (dd, J = 11.2, 2.7 Hz, 1H), 5.13 (dd, J = 11.3, 5.6 Hz, 1H), 4.94 (d, J = 5.7 Hz, 1H), 4.28 (s, 1H), 4.16 (t, J = 6.1 Hz, 1H), 3.55 (dd, J = 11.4, 5.8 Hz, 1H), 3.46 (dd, J = 11.5, 6.4 Hz, 1H). ESI-MS m/z calcd for [C20H18BrF3N3O5S]+ (M+H)+: 548.0; found: 548.0.

Example 40


2-Phenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0262] 



[0263] To a solution of 2-phenethyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (80 mg, 0.18 mmol) in DMF (3 mL) were added trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (80 mg, 0.35 mmol), copper(I) iodide (10 mg, 0.05 mmol) and triethylamine (0.12 mL, 0.89 mmol). The mixture was stirred at 100 °C for 1 h. Water (10 mL) was added and the mixture was filtrated through celite. The filtrate was dilluted by CH2Cl2 (40 mL), washed with sat. NaCl solution. The organic layers were dried over anhydrous Na2SO4 and concentrated in vacuo. The residue was dissolved in methanolic NaOMe (8 mL, pH = 9-10) and stirred at 15 °C for 2 h. Neutralization with H+ resin, filtered and concentrated to a syrup. The crude product was purified by prep-HPLC to afford 2-phenethyl 3-(4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazole)-3-deoxy-1-thio-α-D-galactopyranoside 35 mg as a white solid.
1H NMR (500 MHz, Methanol-d4) δ 8.50 (s, 1H), 7.64 (d, J = 7.0 Hz, 1H), 7.62 (d, J = 6.5 Hz, 1H), 7.28-7.26 (m, 4H), 7.20-7.18 (m, 1H), 5.62 (d, J = 5.5 Hz, 1H), 4.94(dd, J = 11.0, 2.5 Hz, 1H), 4.83 (dd, J = 11.5, 5.5 Hz, 1H), 4.40 (t, J = 6.0 Hz, 1H), 4.30 (d, J = 2.5 Hz, 1H), 3.77-3.75 (m, 2H), 2.99-2.95 (m, 3H), 2.89-2.87 (m, 1H). ESI-MS m/z calcd for [C22H23F3N3O4S]+ (M+H)+: 482.1; found: 482.0.

Example 41 (compound not according to the invention)


3,4-Dichlorophenyl 3-O-[(2-amino-(4-chlorophenyl) pyrimidin-6-yl) methylene] - 1-thio-α-D-galactopyranoside



[0264] 



[0265] To a solution of 3,4-dichlorophenyl 2,4,6-tri-O-acaetyl-3-O-[(4-chlorophenyl)-4-oxobut-2-ynyl]-1-thio-α-D-galactopyranoside (140 mg, 0.22 mmol) in tetrahydrofuran (10 mL) was added guanidine hydrochloride (52 mg, 0.54 mmol) and K2CO3 (90 mg, 0.65 mmol). The reaction vessel was purged 3 times with nitrogen. Then the mixture was heated to reflux with stirring for 20 h. The reaction mixture was concentrated by rotovapor. Dilute with MeOH (10 mL), concentrated by rotovapor. The residue was dilute with MeOH (5 mL), filtered and the flitrate was purified by prep-HPLC to yield 3, 4-dichlorophenyl-3-O-[(2-amino-(4-chlorophenyl) pyrimidin-6-yl) methylene] -1-thio-α-D-galactopyranoside 22 mg (18.1%) as a white solid.
1H NMR (500 MHz, Methanol-d4) δ 8.13 (d, J = 8.6 Hz, 2H), 7.76 (d, J = 2.0 Hz, 1H), 7.56 - 7.42 (m, 5H), 5.74 (d, J = 5.6 Hz, 1H), 4.65-4.75 (dd, 2H), 4.42-4.48(m, 1H), 4.33 - 4.22 (m, 2H), 3.76 (dd, J = 15.0, 6.1 Hz, 2H), 3.65 (dd, J = 10.1, 3.0 Hz, 1H). ESI-MS m/z calcd for [C23H23Cl3N3O5S]+ [M+H]+: 558.1; found: 558.1.

Synthesis of intermediates i1-i41


i1) 3,4-Dimethylphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0266] 



[0267] 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside was synthesized from 1,2,4,6-tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside according to a known literature procedure (Farkas et al, 1976). However, the product was used without purification in the next step.



[0268] Synthesized according to a known literature procedure (Ramos-Soriano et al, 2013). 3,4-Dimethylbenzenethiol (0.056 mL, 0.42 mmol) was dissolved in anhydrous DMF (1 mL) and stirred at rt. NaH (21 mg, 57-63%, 0.55 mmol) was added and the mixture was stirred at rt for one hour and then added to 3-azido-3-deoxy-β-D-galactopyranoside chloride (73 mg, 0.21 mmol) dissolved in anhydrous DMF (1 mL) and the mixture was stirred at 55 °C for one hour and then at rt for 18 hours. The reaction mixture was diluted with EtOAc and washed four times with brine, the combined water phase was extracted once with EtOAc and the combined organic phase was dried (Na2SO4) and concentrated. The residue was purified by flash chromatography, 7-75% EtOAc in petroleum ether to give 46 mg (49%) of 3,4-dimethylphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside as an amorphous white solid. 1H NMR (400 MHz, Chloroform-d) δ 7.31 - 7.22 (m, 2H), 7.11 (d, J = 7.8 Hz, 1H), 5.93 (d, J = 5.5 Hz, 1H), 5.52 (d, J = 2.9 Hz, 1H), 5.31 (dd, J = 10.9, 5.5 Hz, 1H), 4.75 (t, J = 6.4 Hz, 1H), 4.15 (dd, J = 11.5, 5.6 Hz, 1H), 4.11 - 3.98 (m, 2H), 2.28 (s, 6H), 2.23 (s, 3H), 2.20 (s, 3H), 2.04 (s, 3H). ESI-MS m/z calcd for [C20H25N3O7SNa]+ (M+Na)+: 474.1; found: 474.0.

Intermediate i2-i9 i11-i16 were made using a similar procedure as i1 using the corresponding arylthiol.


i2) 3-Bromophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0269] 



[0270] Yield 31%. 1H NMR (400 MHz, Chloroform-d) δ 7.52 (s, 1H), 7.35 - 7.24 (m, 2H), 7.08 (t, J = 7.9 Hz, 1H), 5.90 (d, J = 5.5 Hz, 1H), 5.38 (d, J = 3.1 Hz, 1H), 5.19 (dd, J = 10.9, 5.5 Hz, 1H), 4.58 - 4.49 (m, 1H), 4.03 (dd, J = 11.6, 5.0 Hz, 1H), 3.97 - 3.81 (m, 2H), 2.08 (d, J = 8.3 Hz, 6H), 1.90 (s, 3H). ESI-MS m/z calcd for [C18H20BrN3O7SNa]+ (M+Na)+: 524.0; found: 524.0.

i3) 3-Ethoxyphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0271] 



[0272] Yield 29%. 1H NMR (400 MHz, Chloroform-d) δ 7.36 - 7.22 (m, 1H), 7.08 (d, J = 6.7 Hz, 2H), 6.91 - 6.84 (m, 1H), 6.05 (d, J = 5.5 Hz, 1H), 5.54 (d, J = 3.0 Hz, 1H), 5.35 (dd, J = 11.0, 5.5 Hz, 1H), 4.74 (t, J = 6.3 Hz, 1H), 4.19 (dd, J = 11.5, 5.3 Hz, 1H), 4.13 - 3.99 (m, 4H), 2.25 (s, 3H), 2.23 (s, 3H), 2.05 (s, 3H), 1.47 (t, J = 7.0 Hz, 3H). ESI-MS m/z calcd for [C20H25N3O8SNa]+ (M+Na)+: 490.1; found: 490.0.

i4) 4-Pyridyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0273] 



[0274] Yield 29%. 1H NMR (400 MHz, Chloroform-d) δ 8.41 (d, J = 6.1 Hz, 2H), 7.31 - 7.24 (m, 2H), 6.20 (d, J = 5.6 Hz, 1H), 5.43 (d, J = 3.1 Hz, 1H), 5.29 (dd, J = 11.0, 5.6 Hz, 1H), 4.54 - 4.41 (m, 1H), 4.08 (dd, J = 11.6, 5.0 Hz, 1H), 4.00 - 3.87 (m, 2H), 2.13 (s, 3H), 2.12 (s, 3H), 1.82 (s, 3H). ESI-MS m/z calcd for [C17H21N4O7S]+ (M+H)+: 425.1; found: 425.0.

i5) 2,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-l-thio-α-D-galactopyranoside



[0275] 



[0276] Yield 39%. 1H NMR (400 MHz, Chloroform-d) δ 7.57 - 7.45 (m, 2H), 7.26 (dd, J = 8.4, 2.0 Hz, 1H), 6.07 (d, J = 5.5 Hz, 1H), 5.53 (d, J = 2.3 Hz, 1H), 5.35 (dd, J = 11.0, 5.5 Hz, 1H), 4.65 (t, J = 6.2 Hz, 1H), 4.21 - 3.96 (m, 3H), 2.24 (s, 3H), 2.20 (s, 3H), 2.01 (s, 3H). ESI-MS m/z calcd for [C18H19Cl2N3O7SNa]+ (M+Na)+: 514.0; found: 514.0.

i6) 4-Acetanilidyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0277] 



[0278] Yield 56%. 1H NMR (400 MHz, Chloroform-d) δ 7.51 - 7.38 (m, 4H), 5.86 (d, J = 5.5 Hz, 1H), 5.47 (d, J = 2.4 Hz, 1H), 5.25 (dd, J = 10.9, 5.5 Hz, 1H), 4.69 (t, J = 6.3 Hz, 1H), 4.15 - 4.06 (m, 1H), 4.04 - 3.93 (m, 2H), 2.19 (s, 3H), 2.17 (s, 3H), 2.15 (s, 3H), 2.02 (s, 3H). ESI-MS m/z calcd for [C20H24N4O8SNa]+ (M+Na)+: 503.1; found: 503.1.

i7) 4-Methoxyphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0279] 



[0280] Yield 52%. 1H NMR (400 MHz, Chloroform-d) δ 7.28 (d, J = 8.6 Hz, 2H), 6.74 (d, J = 8.7 Hz, 2H), 5.68 (d, J = 5.5 Hz, 1H), 5.37 (d, J = 2.6 Hz, 1H), 5.14 (dd, J = 10.9, 5.5 Hz, 1H), 4.61 (t, J = 6.3 Hz, 1H), 4.00 (dd, J = 11.5, 5.5 Hz, 1H), 3.95 - 3.83 (m, 2H), 3.69 (s, 3H), 2.09 (s, 3H), 2.04 (s, 3H), 1.92 (s, 3H). ESI-MS m/z calcd for [C19H23N3O8SNa]+ (M+Na)+: 476.1; found: 476.0.

i8) 2,3-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0281] 



[0282] Yield 29%. 1H NMR (400 MHz, Chloroform-d) δ 7.28 (dd, J = 7.9, 1.2 Hz, 1H), 7.20 (dd, J = 8.0, 1.3 Hz, 1H), 6.98 (t, J = 8.0 Hz, 1H), 5.91 (d, J = 5.6 Hz, 1H), 5.31 (d, J = 3.2 Hz, 1H), 5.14 (dd, J = 11.0, 5.6 Hz, 1H), 4.42 (t, J = 6.3 Hz, 1H), 3.97 - 3.75 (m, 3H), 2.01 (s, 3H), 1.98 (s, 3H), 1.76 (s, 3H). ESI-MS m/z calcd for [C18H19Cl2N3O7SNa]+ (M+Na)+: 514.0; found: 513.9.

i9) 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0283] 



[0284] Yield 57%. ESI-MS m/z calcd for [C18H19Cl2N3O7SNa]+ (M+Na)+: 514.0; found: 513.9.

i11) 3-Methoxyphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0285] 



[0286] Yield 22%. ESI-MS m/z calcd for [C19H23N3NaO8S]+ (M+Na)+: 476.1; found: 476.0.

i12) 2-Naphtyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0287] 



[0288] Yield 22%. ESI-MS m/z calcd for [C22H23N3NaO7S]+ (M+Na)+: 496.1; found: 496.1.

i13) 3-Methylphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0289] 



[0290] Yield 46%. ESI-MS m/z calcd for [C19H23N3NaO7S]+ (M+Na)+: 460.1, found: 460.1.

i14) 3-Trifluoromethylphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0291] 



[0292] Yield 19%. ESI-MS m/z calcd for [C19H20F3N3NaO7S]+ (M+Na)+: 514.1, found: 514.1.

i15) 4-Bromophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0293] 



[0294] Yield 19%. ESI-MS m/z calcd for [C18H20BrN3NaO7S]+ (M+Na)+: 524.0; found: 524.1.

i16) 3,5-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0295] 



[0296] Yield 15%. ESI-MS m/z calcd for [C18H23Cl2N4O7S]+ (M+NH4)+: 509.1; found: 509.1.

i22) 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-α-D-galactopyranoside



[0297] 



[0298] 1,2,4,6-tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (155 mg, 0.42 mmol) was dissolved in 1,2-dichloroethane (5 mL) and 3,4-dichlorophenol (131 mg, 0.80 mmol) was added followed by BF3·OEt2 (0.155 mL, 1.26 mmol). The mixture was heated to 60 °C and after eighteen hours 3,4-dichlorophenol (138 mg, 0.85 mmol) and BF3·OEt2 (0.155 mL, 1.26 mmol) was added. After an additional 72 h the mixture was allowed to cool to rt and NEt3 (2 mL) was added and the mixture was concentrated down. The residue was purified by flash chromatography, 6-75% EtOAc in petroleum ether to give 95 mg (48%) of 2,3-dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-α-D-galactopyranoside as an amorphous white solid. 1H NMR (400 MHz, Chloroform-d) δ 7.28 (d, J = 8.9 Hz, 1H), 7.15 (d, J = 2.8 Hz, 1H), 6.84 (dd, J = 8.9, 2.8 Hz, 1H), 5.62 (d, J = 3.5 Hz, 1H), 5.41 (d, J = 30 Hz, 1H), 5.11 (dd, J = 10.9, 3.5 Hz, 1H), 4.13 (dd, J = 10.8, 3.5 Hz, 2H), 4.03 (dd, J = 11.5, 5.2 Hz, 1H), 3.93 (dd, J= 11.4, 7.6 Hz, 1H), 2.10 (s, 3H), 2.07 (s, 3H), 1.90 (s, 3H).). ESI-MS m/z calcd for [C18H19Cl2N3O8Na]+ (M+Na)+: 498.0; found: 498.0.

Intermediate i10, i23 were made using a similar procedure as i22 using the corresponding arylthiol.


i10) Benzyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0299] 



[0300] Yield 20%. ESI-MS m/z calcd for [C19H23N3O7SNa]+ (M+Na)+: 460.1, found: 460.1.

i23) 4-Chlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0301] 



[0302] Yield 34%. 1H NMR (400 MHz, Chloroform-d) δ 7.43 - 7.30 (m, 2H), 7.30 - 7.21 (m, 2H), 5.91 (d, J = 5.5 Hz, 1H), 5.45 (d, J = 2.7 Hz, 1H), 5.25 (ddd, J = 11.0, 5.5, 1.3 Hz, 1H), 4.61 (t, J = 6.0 Hz, 1H), 4.08 (ddd, J = 11.5, 5.2, 1.4 Hz, 1H), 4.03 - 3.90 (m, 2H), 2.16 (s, 3H), 2.13 (s, 3H), 1.96 (s, 3H). ESI-MS m/z calcd for [C18H20ClN3O7SNa]+ (M+Na)+: 480.1; found: 480.0.

i27) phenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside


2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside



[0303] 



[0304] To a stirred suspension of 1,2,4,6-tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (1.0 g, 2.68 mmol) and PCl5 (610 mg, 2.93 mmol) in dry DCM (10 mL), BF3·Et2O (15 µL) was added. After stirring for 20 min, TLC analysis showed complete disappearance of the starting material. The reaction mixture was diluted with DCM (50 mL) and then washed with ice-cold water, sat. ice-cold NaHCO3 solution (2×30 mL), and again ice-cold water successively, dried over anhydrous Na2SO4 and evaporated under reduced pressure to afford the crude product 2,4,6-triO-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside 850 mg as a white solid.
1H NMR (500 MHz, CDCl3) δ 5.48 (dd, J = 3.0, 1.0 Hz, 1H), 5.34 (dd, J = 10.0, 9.0 Hz, 1H), 5.24 (d, J = 8.5 Hz, 1H), 4.18 (dd, J = 11.0, 5.5 Hz, 1H), 4.10 (dd, J = 11.5, 6.5 Hz, 1H), 3.98 (m, 1H), 3.60 (dd, J = 10.0, 3.5 Hz, 1H), 2.20 (s, 3H), 2.17 (s, 3H), 2.07 (s, 3H). ESI-MS m/z calcd for [C12H21N4O8]+ (M-Cl+OH+NH4)+: 349.1; found: 349.1.



[0305] To a stirred solution of benzenethiol (190 mg, 1.72 mmol) in dry DMF (4 mL) was added NaH (60 mg, 60% in mineral oil, 1.67 mmol) at 0 °C. The mixture was stirred at r.t for 30 min. Then 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (200 mg, 0.57 mmol) was added. The mixture was stirred at 50 °C for 10 h and then overnight at r.t. The solvent was removed and the resulting crude was diluted with DCM and washed with water and brine. The organic layer was dried over anhydrous Na2SO4, filtered and evaporated. The crude product was purified by chromatography column (PE:EtOAc = 2:1) to afford the product mixture 160 mg as white solid.[α-anomer : β-anomer = 100:8].
1H NMR (α-anomer, 500 MHz, CDCl3) δ 7.46-7.44 (m, 2H), 7.33-7.29 (m, 3H), 5.97 (d, J = 6.0 Hz, 1H), 5.48 (d, J = 2.0 Hz, 1H), 5.28 (dd, J = 11.0, 5.5 Hz, 1H), 4.69 (t, J = 6.5 Hz, 1H), 4.11 (dd, J = 11.0, 6.5 Hz, 1H), 4.03-3.97 (m, 2H), 2.19 (s, 3H), 2.16 (s, 3H), 1.97 (s, 3H). ESI-MS m/z calcd for [C18H25N4O7S]+ (M+NH4)+: 441.1; found: 441.1.

i28) 3-chloro-4-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0306] 



[0307] To a stirred solution of 3-chloro-4-fluoro-benzenethiol (280 mg, 1.72 mmol) in dry DMF (4 mL) was added NaH (60 mg, 60% in mineral oil, 1.67 mmol) at 0 °C. The mixture was stirred at r.t for 30 min. Then 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (200 mg, 0.57 mmol) was added. The mixture was stirred at 50 °C for 10 h and then overnight at r.t. Then, the solvent was removed and the resulting crude was diluted with DCM and washed with water and brine. The organic layer was dried over anhydrous Na2SO4, filtered and evaporated. The crude product was purified by chromatography column (PE:EtOAc = 3:1) to afford the α-anomer product 210 mg as a white solid.
1H NMR (500 MHz, CDCl3) δ 7.54 (dd, J = 6.5, 2.0 Hz, 1H), 7.33 (m, 1H), 7.10 (t, J = 9.0 Hz, 1H), 5.90 (d, J= 5.5 Hz, 1H), 5.48 (d, J = 2.0 Hz, 1H), 5.26 (dd, J = 11.5, 5.5 Hz, 1H), 4.64 (t, J = 6.0 Hz, 1H), 4.12 (dd, J = 12.0, 5.0 Hz, 1H), 4.02 (dd, J = 12.0, 8.0 Hz, 1H), 3.93 (dd, J = 11.0, 3.5 Hz, 1H), 2.20 (s, 3H), 2.16 (s, 3H), 2.03 (s, 3H). ESI-MS m/z calcd for [C18H23ClFN4O7S]+ (M+NH4)+: 493.1; found: 493.0.

i29) 4-tolyl-2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside


4-tolyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0308] 



[0309] To a solution of 1,2,4,6-tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (200 mg, 0.54 mmol) in 1,2-dichloroethane (10 mL) were added boron trifluoride etherate (0.4 mL, 3.2 mmol), 3A molecular sieve, 4-methylbenzenethiol (144 mg, 1.1 mmol) and the solution was stirred at 60 °C for 17 h. TLC (silica gel, PE:EtOAc=3:1, UV, PMA) analysis indicated the total consumption of the starting material. The mixture was filtered and the filtrate was poured on sat. NaHCO3 (20 mL) and DCM (50 mL). The organic phase was washed with water (20 mL) and brine (20 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue and the residue was purified by flash column chromatography (silica gel, PE:EtOAc = 3:1) to give 4-tolyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside 70 mg and as a side product the corresponding β-isomer 59 mg as white solid.
1H NMR (α-isomer,500 MHz, CDCl3) δ 7.34 (d, J = 8.5 Hz, 2H), 7.12 (d, J = 7.5 Hz, 2H), 5.88 (d, J = 5.5 Hz, 1H), 5.47 (d, J = 2.5 Hz, 1H), 5.26 (dd, J = 6.0, 5.5 Hz, 1H), 4.70 (t, J = 6.0 Hz, 1H), 4.10 (dd, J = 11.5 Hz, 5.0 Hz, 1H), 4.02-3.96 (m, 2H), 2.33 (s, 3H), 2.19 (s, 3H), 2.15 (s, 3H), 2.00 (s, 3H). ESI-MS m/z calcd for [C19H23N3O7S]+ (M+NH4)]+: 455.1; found: 455.1.

4-tolyl-2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0310] 



[0311] To a solution of 4-tolyl-2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (70 mg, 0.16 mmol) in dry DMF (5 mL) were added N,N-diethylethanamine (0.11 mL, 0. 8 mmol), copper(I) iodide (9.1 mg, 0.05 mmol), trimethyl-[2-(3,4,5 trifluorophenyl) ethynyl]silane (73 mg, 0.32 mmol) and the solution was stirred at 100 °C for 1 h. The reaction mixture was poured on water (50 mL) and filtered. The aqueous phase was extracted with ethyl acetate (2×50 mL), the combined organic phases were washed with water (4×50 mL) and brine (100 mL), dried over anhydrous sodium sulphate. Removal of solvent to gave a residue, which was purified by flash column chromatography (silica gel, PE:EtOAc = 3:1) to afford the title compound 85 mg as a white solid.
1H NMR (500 MHz, CDCl3) δ 7.77 (s, 1H), 7.44 (t, J = 7.0 Hz, 2H), 7.38 (d, J = 7.5 Hz, 2H), 7.16 (d, J = 8.0 Hz, 2H), 6.07 - 6.03 (m, 2H), 5.60 (dd, J = 3.5 Hz, 1.0 Hz, 1H), 5.27 - 5.23 (m, 1H), 4.93 (t, 6.5 Hz, 1H), 4.14 (dd, J = 11.5 Hz, 6.0 Hz, 1H), 4.06 (dd, J = 11.5 Hz, 7.50 Hz, 1H), 2.35 (s, 3H), 2.05 (s, 3H), 2.00 (s, 3H), 1.97 (s, 3H). ESI-MS m/z calcd for [C27H26F3N3O7S]+ (M+H)+: 594.1; found: 594.2.

i30) 4-fluorophenyl-2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside


4-fluorophenyl (2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0312] 



[0313] To a solution of 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (200 mg, 0.57 mmol) in dry DMF (5 mL) were added 4-fluorobenzenethiol (219 mg, 1.7 mmol), sodium hydride (60% in mineral oil, 68 mg, 1.7 mmol). The solution was stirred at 50°C for 17 h. TLC (silica gel, PE:EtOAc=3:1, UV, PMA) analysis indicated the total consumption of the starting material, the reaction mixture was poured on water (50 mL) and EtOAc (50 mL). The organic phase was washed with water (2×50 mL), brine (50 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue, which was purified by flash column chromatography (silica gel, PE:EtOAc = 3:1) to give 4-fluorophenyl (2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside 156 mg as a white solid.
1H NMR (500 MHz, CDCl3) δ 7.46 - 7.43 (m, 2H), 7.04-7.00 (m, 2H), 5.87 (d, J = 5.0 Hz, 1H), 5.48 (d, J = 2.5 Hz, 1H), 5.25 (dd, J = 11.5 Hz, 5.5 Hz, 1H), 4.67 (t, J = 6.0 Hz, 1H), 4.10 (dd, J = 11.0 Hz, 5.5 Hz, 1H), 4.03 - 3.95 (m, 2H), 2.20 (s, 3H), 2.16 (s, 3H), 2.01 (s, 3H). ESI-MS m/z calcd for [C18H20FN3O7S]+ (M+NH4)+: 459.1; found: 459.0.

4-fluorophenyl-2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0314] 



[0315] To a solution of 4-fluorophenyl (2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside 156 mg in DMF (10 mL) were added triethylamine (179 mg, 1.8 mmol), copper(I) iodide (20 mg, 0.11 mmol), trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (161 mg, 0.71 mmol). The solution was stirred at 100 °C for 1 h under N2. TLC (silica gel, PE:EtOAc=3:1, UV, PMA) analysis indicated the total consumption of the starting material. Water (50 mL) and EtOAc (50 mL) were added. The mixture was filtered and the filtrate was extracted with ethyl acetate (2×50 mL), the combined organic phases were washed with water (100 mL) and brine (100 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The crude was purified by flash column chromatography (silica gel, PE : EtOAc = 3:1) to afford product 150 mg as a white solid.
1H NMR (500 MHz, CDCl3) δ 7.77 (m, 1H), 7.51-7.42 (m, 4H), 7.07-7.04 (m, 2H), 6.07 - 6.02 (m, 2H), 5.60 (dd, J = 2.5 Hz, J = 1.0 Hz, 1H), 5.22 (dd, J = 10.5 Hz, 3.0 Hz, 1H), 4.90 (t, J = 7.0 Hz, 1H), 4.15 - 4.05 (m, 2H), 2.06 (s, 3H), 2.02 (s, 3H), 2.00 (s, 3H). ESI-MS m/z calcd for [C26H23F4N3O7S]+ (M+H)]+: 598.1; found: 598.0.

i31) 4-trifluoromethoxyphenyl-2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside


trifluoromethoxyphenyl (2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0316] 



[0317] To a solution of 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (200 mg, 0.57 mmol) in dry DMF (5 mL) were added 4-(trifluoromethoxy) benzenethiol (333 mg, 1.7 mmol), sodium hydride (60% in mineral oil, 68 mg, 1.7 mmol), the solution was stirred at 50 °C for 17 h. TLC (silica gel, PE:EtOAc = 3:1, UV, PMA) analysis indicated the total consumption of the starting material. The reaction mixture was poured on water (50 mL) and EtOAc (50 mL). The organic phase was washed with water (2×50 mL), brine (50 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue, which was purified by flash column chromatography (silica gel, PE:EtOAc = 3:1) to give 4-trifluoromethoxyphenyl (2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside 117 mg as a white solid.
1H NMR (500 MHz, CDCl3) δ 7.50 - 7.47 (m, 2H), 7.17-7.16 (m, 2H), 5.97 (d, J = 6.0 Hz, 1H), 5.48 (d, J = 2.0 Hz, 1H), 5.28 (dd, J = 11.5, 6.0 Hz, 1H), 4.65 (t, J = 6.5 Hz, 1H), 4.13 - 4.10 (m, 1H), 4.03 - 3.95 (m, 2H), 2.19 (s, 3H), 2.17 (s, 3H), 1.97 (s, 3H). ESI-MS m/z calcd for [C19H20F3N3O8S]+ (M+NH4)+: 525.1; found: 525.0.

4-trifluoromethoxyphenyl 2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0318] 



[0319] To a solution of 4-trifluoromethoxyphenyl (2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (117 mg, 0.23 mmol) in DMF (10 mL) were added N,N-diethylethanamine (117 mg, 1.1 mmol), copper(I) iodide (13 mg, 0.07 mmol), trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (105 mg, 0.46 mmol). The solution was stirred at 100 °C for 1 h under N2. TLC (silica gel, PE:EtOAc=3:1, UV, PMA) analysis indicated the total consumption of the starting material. Water (50 mL) and EtOAc (50 mL) were added. The mixture was filtered and the filtrate was extracted with ethyl acetate (2×50 mL). The combined organic phases were washed with water (100 mL) and brine (100 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The crude was purified by flash column chromatography (silica gel, PE:EtOAc = 3:1) to afford 4-trifluoromethoxyphenyl 2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside 150 mg as a white solid.
1H NMR (500 MHz, CDCl3) δ 7.77 (s, 1H), 7.55 - 7.52 (m, 2H), 7.45 - 7.42 (m, 2H), 7.20 (d, J = 8.0 Hz, 2H), 6.13 (d, J = 6.0 Hz, 1H), 6.08 (dd, J = 11.5 Hz, 5.5 Hz, 1H), 5.61 (d, J = 2.5 Hz, 1H), 5.23 (dd, J = 11.5 Hz, 3.0 Hz, 1H), 4.88 (t, J = 6.5 Hz, 1H), 4.17 - 4.06 (m, 2H), 2.06 (s, 3H), 1.98 (s, 3H), 1.97 (s, 3H). ESI-MS m/z calcd for [C27H23F6N3O8S]+ (M+H)+: 664.1; found: 664.0.

example i32-i34 were made using a similar procedure as i22 from the corresponding nucleophile


i32) Phenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-α-D-galactopyranoside



[0320] 



[0321] Yield 37%. 1H NMR (400 MHz, Chloroform-d) δ 7.32 (t, J = 8.0 Hz, 2H), 7.10 - 7.06 (m, 3H), 5.77 (d, J = 3.5 Hz, 1H), 5.51 (d, J = 2.9Hz, 1H), 5.22 (dd, J = 10.9, 3.5 Hz, 1H), 4.36 - 4.25 (m, 2H), 4.14 (dd, J = 11.4, 5.7 Hz, 1H), 4.01 (dd, J = 11.4, 7.2 Hz, 1H), 2.19 (s, 3H), 2.16 (s, 3H), 1.95 (s, 3H). ESI-MS m/z calcd for [C18H21N3O8Na]+
(M+Na)+: 430.1; found: 430.0.

i33) 3-Chlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-α-D-galactopyranoside



[0322] 



[0323] Yield 27%. 1H NMR (400 MHz, Chloroform-d) δ 7.31 - 7.23 (m, 1H), 7.22 - 7.14 (m, 1H), 7.10 (d, J = 8.0 Hz, 1H), 6.99 (dd, J = 8.3, 1.4 Hz, 1H), 5.77 (d, J = 3.4Hz, 1H), 5.56 - 5.50 (m, 1H), 5.24 (dd, J = 10.9, 3.5 Hz, 1H), 4.32 - 4.25 (m, 2H), 4.16 (dd, J = 11.4, 5.3 Hz, 1H), 4.06 (dd, J= 11.4, 7.6 Hz, 1H) 2.22 (s, 3H), 2.19 (s, 3H), 2.01 (s, 3H). ESI-MS m/z calcd for [C18H20ClN3O8Na]+ (M+Na)+: 464.1; found: 464.1.

i34) 4-Chlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-α-D-galactopyranoside



[0324] 



[0325] Yield 52%. 1H NMR (400 MHz, Chloroform-d) δ 7.28 (d, J= 9.0 Hz, 2H), 6.78 (d, J = 8.9 Hz, 2H), 5.73 (d, J = 3.5 Hz, 1H), 5.51 (d, J = 2.9 Hz, 1H), 5.21 (dd, J = 10.9, 3.5 Hz, 1H), 4.31 - 4.23 (m, 2H), 4.14 (dd, J = 11.4, 5.6 Hz, 1H), 4.02 (dd, J = 11.4, 7.3 Hz, 1H), 2.19 (s, 3H), 2.17 (s, 3H), 1.98 (s, 3H). ESI-MS m/z calcd for [C18H20ClN3O8Na]+ (M+Na)+: 464.1; found: 464.1.

example i35-i38 were made using a similar procedure as i1 from the corresponding nucleophile


i35) Cyclohexyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-α-D-galactopyranoside



[0326] 



[0327] Yield 6%. 1H NMR (400 MHz, Chloroform-d) δ 5.82 (d, J = 5.6 Hz, 1H), 5.41 (d, J = 2.4 Hz, 1H), 5.18 (dd, J = 10.9, 5.6 Hz, 1H), 4.57 (t, J = 6.3 Hz, 1H), 4.12 (dd, J = 11.5, 5.5 Hz, 1H), 4.03 (dd, J = 11.4, 7.3 Hz, 1H), 3.88 (dd, J = 10.9, 3.3 Hz, 1H), 2.83 - 2.73 (m, 1H), 2.15 (s, 3H), 2.14 (s, 3H), 2.05 (s, 3H), 2.03 - 1.23 (m, 10H). ESI-MS m/z calcd for [C18H27N3O7SNa]+ (M+Na)+: 452.1; found: 452.1.

i36) 2,4,5-Trichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-α-D-galactopyranoside



[0328] 



[0329] Yield 50%. 1H NMR (400 MHz, Chloroform-d) δ 7.66 (s, 1H), 7.53 (s, 1H), 6.06 (d, J = 5.6 Hz, 1H), 5.49 (d, J = 2.9 Hz, 1H), 5.31 (dd, J = 11.0, 5.6 Hz, 1H), 4.58 (dd, J = 7.3, 5.2 Hz, 1H), 4.11 (dd, J = 11.7, 4.9 Hz, 1H), 4.06 - 3.96 (m, 2H), 2.20 (s, 3H), 2.17 (s, 3H), 1.98 (s, 3H). ESI-MS m/z calcd for [C18H18N3O7SNa]+ (M+Na)+: 548.0; found: 547.8.

i37) 2,5-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0330] 



[0331] Yield 40%. ESI-MS m/z calcd for [C18H19Cl2N3O7SNa]+ (M+Na)+: 514.0; found: 513.9.

i38) 3-Hydroxyphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0332] 



[0333] Not isolated but used in the next step directly. ESI-MS m/z calcd for [C18H21N3NaO8S]+ (M+Na)+: 462.1; found: 462.1.

i40) 2-phenethyl 2, 4, 6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0334] 



[0335] To a stirred suspension of 1,2,4,6-tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (200 mg, 0.54 mmol) in dry CH2Cl2 (5 mL), 2-phenylethanethiol (220 mg, 1.59 mmol) and BF3·Et2O (0.4 mL, 3.24 mmol) were added at 0 °C. The mixture was stirred at 15 °C overnight. After dilluted by CH2Cl2 (40 mL), the mixture was washed with satd. NaHCO3 solution. The organic layers were dried over Na2SO4 and concentrated in vacuo. The residue was purified by chromatography (PE:EtOAc = 3:1) to yield 2-phenethyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-a-D-galactopyranoside 81 mg as a white solid.
1H NMR (500 MHz, CDCl3) δ 7.31-7.17 (m, 5H), 5.75 (d, J= 6.0 Hz, 1H), 5.41 (d, J = 2.5 Hz, 1H), 5.21 (dd, J = 11.0, 5.5 Hz, 1H), 4.49 (t, J = 6.0 Hz, 1H), 4.12 (dd, J = 11.0, 5.0 Hz, 1H), 4.03 (dd, J = 11.5, 7.5 Hz, 1H), 3.66 (dd, J = 11.0, 4.0 Hz, 1H), 2.92-2.74 (m, 4H), 2.17 (s, 3H), 2.16 (s, 3H), 2.13 (s, 3H). ESI-MS m/z calcd for [C20H25N3NaO7S]+ (M +Na)+: 474.1; found: 474.1.

i41) 3, 4-dichlorophenyl-2, 4, 6-tri-O-acaetyl-3-O-[(4-chlorophenyl)-4-oxobut-2-ynyl]-1-thio-α-D-galactopyranoside


1,2,3,4,6-penta-O-acetyl-β-D-galactopyranoside



[0336] 



[0337] A suspension of anhydrous sodium acetate (13.7g, 16.7 mmol) and acetic anhydride (20 mL) was refluxed for 5 minutes. After addition of D-(+)-galactose (3.0 g, 16.7 mmol) refluxing was continued for further 30 min. The hot solution was poured on ice water (300 mL) and the mixture was extracted with DCM (3 × 150 mL). Dried with MgSO4, filtered, and evaporated of the solvent under reduced pressure to afford the crude product. Then the crude product was re-crystallized from hexane/EtOAc (2:1) to yield 1, 2, 3, 4, 6-penta-O-acetyl-β-D-galactopyranoside 4.2 g (64.6%) as a white solid.
1H NMR (500 MHz, CDCl3) δ 5.70 (d, J= 8.3 Hz, 1H), 5.43 (d, J= 2.6 Hz, 1H), 5.34 (dd, J = 10.4, 8.4 Hz, 1H), 5.08 (dd, J = 10.4, 3.4 Hz, 1H), 2.17 (s, 3H), 2.13 (s, 3H), 2.08 - 1.98 (m, 9H).

1-chloro 2,3,4,6-tetra-O-acetyl-β-D-galactopyranoside



[0338] 



[0339] To a stirred suspension of 1, 2, 3, 4, 6-penta-O-acetyl-β-D-galactopyranoside (2.3 g, 5.9 mmol) and PCl5 (1.35 g, 6.5 mmol) in dry CH2Cl2 (20 mL), BF3·Et2O (34 mg, 0.29 mmol) was added. After stirring for 30 min, the reaction mixture was diluted with CH2Cl2 (120 mL) and then washed with ice-cold water (60 mL), saturated ice-cold NaHCO3 solution (2 × 50 mL), and again ice-cold water (2 × 30 mL), dried over anhydrous Na2SO4 and evaporated under reduced pressure. The residue was co-evaporated with toluene to yield 1-chloro-2, 3, 4, 6-tetra-O-acetyl-β-D-galactopyranoside 2.1 g (97.2%) as colorless oil.
1H NMR (500 MHz, CDCl3) δ 5.42 (ddd, J = 19.0, 6.7, 4.9 Hz, 2H), 5.33 - 5.23 (m, 1H), 5.02 (dd, J = 10.2, 3.4 Hz, 1H), 4.17 (d, J = 6.5 Hz, 2H), 4.06 - 3.97 (m, 1H), 2.19 (s, 3H), 2.10 (s, 3H), 2.06 (s, 3H), 2.00 (s, 3H).

3,4-dichlorophenyl 2,3,4,6-tetra-O-acetyl-1-thio-α-D-galactopyranoside



[0340] 



[0341] NaH (60% in mineral oil, 338 mg, and 14.7 mmol) was suspended in DMF (20 mL). 3, 4-dichlorobenzenethiol (2.9 g, 16.3 mmol) was added. After 30 min, 1-chloro 2, 3, 4, 6-tetra-O-acetyl-β-D-galactopyranoside(3.0 g, 8.2 mmol) The mixture was heated to 50 °C for 20 h. The mixture was diluted with CH2Cl2 (100 mL), citric acid (0.5 M, 20 ml) and water (20 mL). The organic phase was washed with water (3 × 30 mL) and concentrated. The residue was purified by column chromatography on silica gel (PE: EtOAc = 3:1) to yield 3, 4-dichloro-phenyl-2, 3, 4, 6-tetra-O-acetyl-1-thio-α-D-galactopyranoside 2.7 g (66%) as a white solid.
1H NMR (500 MHz, CDCl3) δ 7.58 (d, J = 2.1 Hz, 1H), 7.38 (d, J= 8.4 Hz, 1H), 7.32 (d, 1H), 5.99 (d, J = 5.6 Hz, 1H), 5.50 (d, J = 2.4 Hz, 1H), 5.35 (dd, J = 11.0, 5.6 Hz, 1H), 5.25 (dd, J = 11.0, 3.3 Hz, 1H), 4.67 (t, J = 6.2 Hz, 1H), 4.13 - 4.04 (m, 2H), 2.16 (s, 3H), 2.12 (s, 3H), 2.02(s, 3H), 2.00 (s, 3H).

3,4-dichloro-phenyl 1-thio-α-D-galactopyranoside



[0342] 



[0343] To a solution of 3, 4-dichlorophenyl-2, 3, 4, 6-tetra-O-acetyl-1-thio-α-D-galactopyranoside (2.7 g, 5.3 mmol) in methanol (30 ml) was added NaOMe (29 mg, 0.53 mmol) and the solution was stirred at room temperature for another 5 h. After completion, the mixture was neutralized (PH=7) with DOWEX 50w × 8-200 Ion exchange resin and filtered. The filtrate was concentrated to give a residue. The product was crystallized from CH2Cl2 (80 mL) to yield 3,4-dichloro-phenyl-1-thio-α-D-galactopyranoside 1.7 g (94%) as a white solid.
ESI-MS m/z calcd for [C12H18Cl2NO5S]+[M+NH4]+: 358.0; found: 358.0.

3,4-dichlorophenyl-3-O-propargyl-1-thio-α-D-galactopyranoside



[0344] 



[0345] 3,4-dichlorophenyl-1-thio-α-D-galactopyranoside (1.2 g, 3.5 mmol) in dry methanol (30 ml) was added dibutyl(oxo)tin (1.0 g, 4.2 mmol). The mixture was refluxed at 70°C for 6 h. The reaction mixture became clear. The solvent was evaporated and dried in vacuo. 1, 4-dioxane (50 mL), tetrabutylammonium iodide (1.3 g, 3.5 mmol), and methyl 2-bromoacetate (4.2 g, 35.2 mmol) was add to reaction above mixture and continued to heat at 105°C for overnight. The mixture was concentrated and purified by combiflash (EtOAc: PE = 4:1, ISCO 40g, 40ml/min, normal phase sillica, uv254) to yield 3, 4-dichlorophenyl 3-O-propargyl-1-thio-α-D-galactopyranoside 520 mg (39%) as a white solid.
1H NMR (500 MHz, DMSO-d6) 7.75 (d, J = 2.1 Hz, 1H), 7.55 (d, J = 8.5 Hz, 1H), 7.45 (dd, J = 8.4, 2.1 Hz, 1H), 5.69 (d, J = 5.5 Hz, 1H), 5.54 (d, J = 4.7 Hz, 1H), 4.80 (d, J = 5.3 Hz, 1H), 4.65 (t, J = 5.6 Hz, 1H), 4.31 (qd, J = 16.0, 2.4 Hz, 2H), 4.15 (dt, J = 10.1,5.1 Hz, 1H), 4.07-3.91 (m, 2H), 3.62-3.40 (m, 3H), 3.40-3.27 (m, 2H).

3,4-dichlorophenyl-2,4,6-tri-O-acetyl-3-O-propargyl-1-thio-α-D-galactopyranoside



[0346] 



[0347] Acetic anhydride (1.4 g, 13.7 mmol) was added dropwise to a solution of 3,4-dichlorophcnyl-3-O-propargyl-1-thio-α-D-galactopyranoside (520 mg, 1.37 mmol) in anhydrous pyridine (10 mL). The reaction was stirred under nitrogen for 20 h at 10 °C. The mixture was concentrated under reduced pressure, and the residue was co-evaporated with toluene. The residue was purified by column chromatography on silica gel (PE: EtOAc = 2: 1) to yield 3, 4-dichlorophenyl-2,4,6-tri-O-acetyl-3-O-propargyl-1-thio-D-galactopyranoside 530 mg (76.5%) as a yellow oil.
ESI-MS m/z calcd for [C21H26Cl2NO8S]+[M+NH4]+: 522.1; found: 522.0.

3, 4-dichlorophenyl 2, 4, 6-tri-O-acetyl-3-O-[(4-chlorophenyl)-4-oxobut-2-ynyl] - 1-thio-α-D-galactopyranoside



[0348] 



[0349] To a solution of 3,4-dichlorophenyl-2,4,6-tri-O-acetyl-3-O-[(4-chlorophenyl)-4-oxobut-2-ynyl]-1-thio-α-D-galactopyranoside (200 mg, 0.4 mmol) in THF (15 mL) was added benzoyl chloride (69 mg, 0.4 mmol), copper(I) iodide (15 mg, 0.08 mmol), PdCl2(PPh3)2Cl2 (29 mg, 0.04 mmol). The mixture was purged three times with N2. Then the mixture was stirred at 10°C for 20 min. Et3N (40 mg, 0.4 mmol) was added via syringe to the reaction. Then the mixture was continued to stir at 10 °C for 20 h. The reaction was quenched with water (20 mL), extracted with dichloromethane (3 × 50 mL) and the aqueous phase was discarded. The extract was washed with brine (20 mL), dried over Na2SO4, filtered, and concentrated. The crude product was purified by combiflash (EtOAc:PE = 1:20 to 1:5, ISCO, 40g, 40ml/min, normal phase, sillica, uv 254) to yield 3,4-dichlorophenyl-2,4,6-tri-O-acetyl-3-O-[(4-chlorophenyl)-4-oxobut-2-ynyl]-1-thio-α-D-galactopyranoside 160 mg (62.8%) as a yellow oil.
ESI-MS m/z calcd for [C28H29Cl3NO9S]+[M+NH4]+: 660.1; found: 660.0.

Example 42-53 were made from the intermediates i42-i53


Example 42


3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide



[0350] 



[0351] 3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, Example 52 (60 mg) was dissolved in CH2Cl2 (30 mL) and cooled with an ice/water bath. m-Chloroperbenzoic acid (30 mg) was dissolved in CH2Cl2 (5 mL) and added to the first solution. The cooling bath was removed. This mixture was stirred 30 min. Water 30 mL and sat. aq. NaHCO3 (5 mL) was added. The mixture was separated and the organic phase was concentrated in vacuo. The residue was purified by HPLC. Freezedrying afforded the titel compound 14 mg as a white solid. 1H NMR (400 MHz, Methanol-d4) δ 8.48 (s, 1H), 8.03 (t, J = 1.1 Hz, 1H), 7.76 (d, J= 1.1 Hz, 2H), 5.64 (dd, J= 11.2, 2.8 Hz, 1H), 5.15 (dd, J = 11.2, 5.6 Hz, 1H), 4.95 (d, J= 5.6 Hz, 1H), 4.32 - 4.27 (m, 1H), 4.17 (t, J= 6.0 Hz, 1H), 3.61 - 3.45 (m, 2H). ESI-MS m/z calcd for [C20H15Cl2F5N3O5S]+ (M+H)+: 574.00; found: 573.95.

Example 43


5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0352] 



[0353] 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, i43 (450 mg, 0.73 mmol) was dissolved in sodium methoxide/methanol (0.05 M, 20 mL). The reaction mixture was stirred at room temperature for 2h followed by neutralisation (pH 7) by addition of DOWEX 50wx8-200 Ion exchange resin. The reaction mixture was filtered and the filtrate was concentrated to give crude material which was purified by preparative HPLC. The appropriate fractions where combined and lyophilized to give the title compound (220 mg, 61.80%) as a white solid.
1H NMR (400 MHz, DMSO-d6) 8.67 (s, 1H), 8.50(d, J = 2 Hz, 1H), 8.39(d, J = 2 Hz, 1H), 8.05 (t, 1H), 7.73 - 7.69 (m, 2H), 5.84 (dd, J = 12.8, 4.8 Hz, 2H), 5.40 (d, J = 6.4 Hz, 1H), 4.73 - 4.70 (m, 1H), 4.61 (dt, J = 11.2, 5.3 Hz, 2H), 4.12 (t, J = 6.0 Hz, 1H), 3.90 (s, 1H), 3.37 (dd, J = 11.2, 5.6 Hz, 1H), 3.36-3.26 (m, 1H). m/z calcd for [C19H16ClF3N4O4S]+ [M+H]+: 489.0; found: 489.0.

Example 44


5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0354] 



[0355] 5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (910 mg) was dissolved in MeOH (10.0 mL) followed by addition of Sodium methoxide (7.46mg, 0.14 mmol). The mixture was stirred at room temperature for 1 h followed by acidification to pH5-6 using dowex 50 wx8 hydrogen form. The reaction mixture was filtered, washed with MeOH (20 mL) and concentrated in vacuo to afford crude product, which was purified by preparative-HPLC to give the title compound (445 mg, 60.5% yield). 1H NMR (400 MHz, DMSO) δ 8.82 (s, 1H), 8.65 (dd, J = 24.1, 1.7 Hz, 2H), 8.39 - 8.23 (m, 1H), 7.96 - 7.74 (m, 2H), 6.09 - 5.90 (m, 2H), 5.55 (d, J = 6.3 Hz, 1H), 4.91 - 4.80 (m, 1H), 4.80 - 4.67 (m, 2H), 4.27 (t, J = 6.1 Hz, 1H), 4.04 (d, J = 3.7 Hz, 1H), 3.61 - 3.48 (m, 1H), 3.47 - 3.38 (m, 2H).
ESI-MS m/z calcd for [C19H16BrF3N4O4S]+ [M+H] +: 532.0; found: 533.0.

Example 45


3-Chloro-5-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0356] 



[0357] To a solution of 3-Chloro-5-cyanophenyl-2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, i45 (120.00 mg, 47.48 mmol) in methanol (11 mL) and water (2.2 mL) were added TEA (6.6 mL). After completion the reaction mixture was evaporated to dryness and the residue was purified by preparative HPLC to obtain the title compound (20 mg, 15%) as a white solid.
1H NMR (400 MHz, DMSO) δ 8.82 (s, 1H), 8.05 - 7.95 (m, 2H), 7.95 - 7.91 (m, 1H), 7.91 - 7.77 (m, 2H), 6.10 (d, J = 5.1 Hz, 1H), 5.97 (d, J = 4.8 Hz, 1H), 5.55 (d, J = 6.3 Hz, 1H), 4.87-4.69 (m, 3H), 4.24 - 4.16 (m, 1H), 4.05 - 4.00 (m, 1H), 3.55 - 3.48 (m, 1H), 3.44 - 3.38 (m, 1H).
m/z calcd for [C21HI6ClF3N4O4S]+ [M+H]+: 513.0; found: 513.0.

Example 46


3-Chloro-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0358] 



[0359] 3-chloro-4-cyanophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside, i46 (98 mg, 0.20 mmol) was dissolved in acetonitrile (5 mL) and stirred at r.t. under argon. Copper(I) iodide (43 mg, 0.23 mmol) was added and after five minutes 1,2,3-trifluoro-5-[2-(trimethylsilyl)ethynyl]benzen (0.085 mL, 0.41 mmol). After an additional five minutes DIEA (0.040 mL, 0.23 mmol) was added and the mixture was heated to 70 °C. After 2.5 h the reaction mixture was filtered through a short column of silica, eluting with EtOAc and then concentrated down. The residue was dissolved in methanolic sodium methoxide (0.05M, 30 mL) and stirred at r.t. After 18 h acetic acid (2 mL) was added and the mixture was concentrated down. The crude product was purified by preparative HPLC and lyophilized to give 46 mg (44%) of the title compound. 1H NMR (400 MHz, Methanol-d4) δ 8.55 (s, 1H), 7.85 (s, 1H), 7.74 -7.61 (m, 4H), 6.11 (d, J = 4.8 Hz, 1H), 5.05 - 4.94 (m, 2H), 4.38 (t, J = 6.0 Hz, 1H), 4.20 (s, 1H), 3.77 - 3.66 (m, 2H). ESI-MS m/z calcd for [C22H16ClF3N4O4S]+ (M+H)+: 513.1; found: 513.0.

Example 47


3-Chloro-6-fluoro-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0360] 



[0361] To a stirred solution of 3-Chloro-6-fluoro-4-cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, i47 (150 mg, 0.23mmol) in MeOH (5 mL) was added triethylamine (3 mL) and H2O (1 mL) was added at 0 °C. The mixture was stirred at r.t. for 1 hour. The reaction mixture was concentrated and the residue was purified by Preparative HPLC to give 20 mg of the title compound.
1H NMR (400 MHz, DMSO) δ 8.83 (s, 1H), 8.11 - 8.03 (m, 2H), 7.92 -7.78 (m, 2H), 6.29 (d, J = 5.5 Hz, 1H), 6.19 - 6.09 (m, 1H), 5.69 - 5.51 (m, 1H), 4.95 - 4.88 (m, 1H), 4.86 - 4.77 (m, 1H), 4.73 - 4.60 (m, 1H), 4.13 - 3.95 (m, 2H), 3.52 - 3.43 (m, 2H).
ESI-MS m/z calcd for [C21H16ClF4N4O4S]+ (M+H)+: 531.1; found: 531.1.

Example 48


3-Bromo-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0362] 



[0363] 3-Bromo-4-cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-a-D-galactopyranoside (100 mg) was dissolved in MeOH (4 mL). TEA (2.4 ml) and H2O (0.8 ml) was added. The mixture was stirred at rt over night followed by purification using preparative HPLC to give 20 mg (24.5%) of the title compound as white solid.
1H NMR (400 MHz, DMSO) δ 8.82 (s, 1H), 8.03 (d, J = 1.6 Hz, 1H), 7.94 - 7.80 (m, 3H), 7.69 (dd, J = 8.3, 1.6 Hz, 1H), 6.20 (d, J = 5.0 Hz, 1H), 6.00 (d, J = 4.6 Hz, 1H), 5.58 (d, J = 6.3 Hz, 1H), 4.92 - 4.72 (m, 2H), 4.69 (t, J = 5.6 Hz, 1H), 4.14 (t, J = 6.3 Hz, 1H), 4.02 (d, J = 4.4 Hz, 1H), 3.59 - 3.50 (m, 1H), 3.43 - 3.37 (m, 2H).
ESI-MS m/z calcd for [C21H17BrF3N4O4S]+ (M+H)+: 557.0; found: 557.0.

Example 49


5-Bromo-6-trifluoromethyl-pyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0364] 



[0365] To a solution of 5-Bromo-6-trifluoromethyl-pyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)- 1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, i49 (90mg, 0.12 mmol) in MeOH (5mL) was added Sodium methoxide (0.67 mg, 0.01 mmol) at room temperature. The reaction mixture was stirred at room temperature for 1 h. The mixture was acidified with dowex 50 wx8 hydrogen form to pH 5∼6. The solution was filtered, washed with MeOH (20 mL) and concentrated in vacuo to afford crude product, which was purified by Preparative HPLC to afford the title compound (12.77 mg, 17 % yield).
1H NMR (400 MHz, MeOD) δ 8.65 (d, J = 1.8 Hz, 1H), 8.46 (s, 1H), 8.43 - 8.38 (m, 1H), 7.69 -7.46 (m, 2H), 6.03 (d, J = 5.1 Hz, 1H), 5.00 - 4.85 (m, 2H), 4.31 (t, J = 6.1 Hz, 1H), 4.10 (d, J= 1.8 Hz, 1H), 3.68 - 3.54 (m, 2H).
ESI-MS m/z calcd for [C20H15BrF6N4O4S]+ (M+H)+: 600.0; found: 601.0.

Example 50


5-Chloro-6-cyano-pyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0366] 



[0367] To a solution of 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-a-D-galactopyranoside, i50 (12.00 mg, 0.02 mmol) in methanol (0.5 mL) and water (0.1mL) were added TEA (0.3 mL). Upon completion the reaction mixture was evaporated to dryness and the residue was purified by preparative-HPLC to obtain the title compound (1.27 mg). m/z calcd for [C20H15ClF3N5O4S]+ [M+H]+: 514.0; found: 514.0.
1H NMR (400 MHz, MeOD) δ 8.62(d, J = 2 Hz, 1H), 8.46 (s, 1H), 8.40 (d, J = 2 Hz, 1H), 7.56 (m, 2H), 6.11 (d, J =1.2 Hz, 1H), 4.95(dd, J = 11.6, 2.8 Hz, 1H), 4.88 (dd, J =11.2, 5.2 Hz, 1H), 4.25 (t, J = 12, 6 Hz, 1H), 4.08 (d, J = 1.6 Hz, 1H), 3.60 (d, J = 6 Hz, 2H).

Example 51


5-Chloro-6-cyano-pyridazin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0368] 



[0369] To a solution of acetyl chloride (0.5 ml) in methanol (5 mL) was added 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, i51 (15 mg). The reaction was stirred for 4 h at room temperature. Followed by purification by preparative-TLC (DCM/MeOH=15/1) to obtain 0.95 mg of the title compound. m/z calcd for [C19H14ClF3N6O4S]+ [M+H]+: 515.0; found: 515.0.
1H NMR (400 MHz, MeOD) δ 8.60(s, 1H), 8.30 (s, 1H), 7.68 (dd, J = 8.8, 6.8 Hz, 2H), 7.56 (d, J = 4 Hz, 1H), 5.11 (d, J=3.6 Hz, 2H), 4.20(m, 2H), 3.71(m, 2H).

Example 52


3,4-Dichlorophenyl 3-deoxy-1-thio-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0370] 



[0371] 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (214 mg) was dissolved in MeCN (10 mL). Copper(I) iodide (15 mg) and pentafluorophenylacetylene (0.130 mL) were added and the mixture degassed (nitrogen). After 5 min, DIEA (0.300 mL) was added and the resulting mixture was stirred over night. The reaction mixture was filtered through a small plug of silica and concentrated in vacuo. The residue was suspended in MeOH (40 mL). 1M sodium methoxide in MeOH (1 mL) was added. After stirring at r.t. 2 h, acetic acid (1 mL) was added and the mixture concentrated in vacuo. The residue was dissolved in a small volume of MeCN/water, filtered and purified by HPLC (C18/H2O:MeCN:0.1% TFA). Freezedrying afforded the title compound as a white powder (174 mg). 1H NMR (400 MHz, Methanol-d4) 8.49 (d, J= 1.6 Hz, 1H), 7.80 (d, J= 2.0 Hz, 1H), 7.57-7.44 (m, 2H), 5.85 (d, J= 5.3 Hz, 1H), 5.06 (dd, J= 11.4, 2.8 Hz, 1H), 4.93 (dd, J = 11.4, 5.4 Hz, 1H), 4.50 (t, J= 6.0 Hz, 1H), 4.23 (s, 1H), 3.79-3.64 (m, 2H). ESI-MS m/z calcd for [C20H15Cl2F5N3O4S]+ (M+H)+: 558.00; found: 558.05.

Example 53


3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone



[0372] 



[0373] Following purification by HPLC in Example 42 7 mg of the title compound was isolated.
1H NMR (400 MHz, Methanol-d4) δ 8.50 (s, 1H), 8.16 (d, J = 2.1 Hz, 1H), 7.92 (dd, J = 8.4, 2.1 Hz, 1H), 7.80 (d, J = 8.4 Hz, 1H), 5.82 (dd, J = 11.4, 2.8 Hz, 1H), 5.34 (d, J = 6.4 Hz, 1H), 5.15 (dd, J = 11.5, 6.4 Hz, 1H), 4.53 (t, J = 6.0 Hz, 1H), 4.31 (s, 1H), 3.60 (qd, J = 11.5, 6.0 Hz, 2H). ESI-MS m/z calcd for [C20H15Cl2F5N3O6S]+ (M+H)+: 589.99; found: 590.00.

Example 54


5-Methoxy-6-cyano-pyridazin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0374] 



[0375] Following purification by preparative-TLC (DCM/MeOH=15/1) in Example 51, 0.5 mg of the title compound was isolated. m/z calcd for [C20H17F3N6O5S]+ [M+H]+: 511.0; found: 511.0.
1H NMR (400 MHz, DMSO-d6) 8.47 (s, 1H), 7.56(dd, J = 8.8, 6.8 Hz, 2H), 7.49(s, 1H), 6.32(d, J = 4 Hz, 1H), 4.95 (m, 2H), 4.14 (m, 1H), 4.08 (m, 4H), 3.59 (d, J = 6 Hz, 1H).

Example 55


5-Hydroxy-6-cyano-pyridazin-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0376] 



[0377] To a solution of acetyl chloride (2 ml) in methanol (20 mL) was added 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, i50
(40 mg). The reaction was held at room temperature with stirring on for 4 h. The reaction mixture was concentrated and purified by preparative HPLC to obtain 7 mg of the title compound.
m/z calcd for [C19H15F3N6O5S]+ [M+H]+: 497.0; found: 497.0.
1H NMR (400 MHz, DMSO-d6) 8.59 (s, 1H), 7.68(dd, J = 8.8, 6.4 Hz, 2H), 7.28(s, 1H), 6.35 (d, J = 4 Hz, 1H), 7.50 (d, J = 4 Hz, 2H), 4.27 (t, J = 12.4, 6 Hz, 1H), 4.22 (s, 1H), 3.75 - 3.73 (m, 2H).

Synthesis of intermediates i43-i51


i43) 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


O-[(5-chloro-3-pyridyl)] N,N-dimethylcarbamothioate



[0378] 



[0379] To a solution of 5-chloropyridin-3-ol (10 g, 0.10 mol) in N,N-dimethylformamide (200mL) was added NaH (1.90 g, 0.10 mol) at 0°C. The reaction mixture was stirred at 0°C 30 minutes followed by addition of Dimethylthiocarbamoyl chloride (10.50 g, 0. 10 mol) followed by stirring at room temperature 20h. The reaction was quenched with water (500 mL) and extracted with dichloromethane (500 mL X 3). The combined organics were washed with brine (400 mL X 3), dried over Na2SO4, filtered and the solvents where removed in vacuo. The crude product was purified by chromatography on a combiflash (EtOAc: PE=1:5) to give O-[(5-chloro-3-pyridyl)] N,N-dimethylcarbamothioate 6.2 g (28.7 %) as brown oil.
m/z calcd for [C8H9ClN2OS]+ [M+H]+: 217.0; found: 217.0.

S-[(5-chloro-3-pyridyl)] N,N-dimethylcarbamothioate



[0380] 



[0381] O-[(5-chloro-3-pyridyl)] N,N-dimethylcarbamothioate (6.2 g, 28.7 mmol) was taken up to in phenoxybenzene (30 mL) and added to 5 mL of refluxing phenoxybenzene. After 2h, the reaction mixture was cooled and passed through 200 g SiO2 to remove the phenoxybenzene, subsequent elution with PE:EtOAc=1:2 gave S-[(5-chloro-3-pyridyl)] N,N-dimethylcarbamothioate 4.5 g (73%) as yellow solid.
m/z calcd for [C8H9ClN2OS]+[M+H]+: 217.0; found: 217.0.

5-chloropyridine-3-thiol



[0382] 



[0383] S-[(5-chloro-3-pyridyl)] N,N-dimethylcarbamothioate (3.5 g, 16.20 mmol) and NaOH (3.24 g, 81 mmol) was taken up into 160 mL ethanol/water = 3:1 and heated at reflux for 2h. The reaction mixture was concentrated to about 100 mL. EtOAc (300 mL) was added and the pH was adjusted to about 6 by addition of HCl(2M). The organic layer was isolated and dried over Na2SO4, concentrated, purified by chromatography on a combiflash (EtOAc:PE =1:5 to 1:2, ISCO, 40g, 40 ml/min, normal phase sillica, uv254) to give 5-chloropyridine-3-thiol 2.0 g (85%) as yellow oil.
m/z calcd for [C5H4ClNS]-[M-H]-: 144.0; found: 144.0.

5-Chloropyridin-3-yl 2,4,6-tri-O-Acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0384] 



[0385] NaH (303.44 mg, 7.58 mmol) was suspended in DMF (25 mL). The 5-chloropyridine-3-thiol (1000 mg, 6.89 mmol) was added. After 30 min, the 3-azido-3-deoxy-2,4,6-tri-O-acetyl-1-chloro-β-D-galactopyranoside (1923.68 mg,5.51 mmol) was added. The mixture was heated to 50 °C 3h. The mixture was diluted with DCM (150 mL), 0.5M citric acid (150 mL) and water (150 mL). The organic phase was washed with water (100 mL) and concentrated. The residue was purified by column chromatography (PE:EtOAc = 3:1) to give the title compound 900 mg (28.52%) as a white solid.
m/z calcd for [C17H19ClN4O7S]+ [M+H]+: 459.0; found: 459.0.

1,3-dideoxy-2,4,6-tri-O-acetyl-1-(5-chloropyridine-3-yl-thio)-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0386] 



[0387] To a solution of 5-Chloropyridin-3-yl 2,4,6-tri-O-Acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (600 mg, 1.30 mmol) in N,N-dimethylformamide (16 mL) was added trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (597.00 mg, 2.62 mmol), and Copperiodide (74.70 mg, 0.4 mmol). The reaction vessel was purged 3 times with nitrogen. After completion the reaction was quenched with water (100 mL) and the reaction mixture was extracted with dichloromethane (100 mL X 3). The combined organics where washed with brine (50 mL X 1), dried over Na2SO4, filtered, and the solvents where evaporated in vacuo. The crude product was purified by combiflash (EtOAc:PE =1:10 to 1:2, ISCO 40g, 40ml/min, normal phase silica, UV254) to give the title compound 450 mg (56%) as a yellow solid.
m/z calcd for [C25H22ClF3N4O7S]+ [M+H]+: 615.0; found: 615.0.

i44) 5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


O-[(5-bromo-3-pyridyl)] N,N-dimethylcarbamothioate



[0388] 



[0389] To a solution of 5-bromopyridin-3-ol (17.4 g, 0.10 mol) in DMF (0.15 L) was added sodium hydride (2.64 g, 0.11 mol, 96% in mineral oil) at 0 °C, followed by stirring at 0 °C for 30 min. Dimethylthiocarbamoyl chloride (14.83 g, 0.12 mol) was added to the reaction mixture followed by stirring at room temperature over night. LC-MS analysis indicated formation of the target compound. The reaction mixture was quenched with water(100 mL) followed by extraction with EtOAc (100 mL X 3). The combined organic layers were washed with brine (100 mL), dried over Na2SO4 and concentrated in vacuo. The crude product was purified by flash chromatography on a Biotage (EtOAc/PE=5% ∼40%, ISCO 120g, 50 mL/min, normal phase silica gel,uv254) to afford the target compound O-[(5-bromo-3-pyridyl)] N,N-dimethylcarbamothioate(9.93 g, 36.5 % yield) as yellow oil.
ESI-MS m/z calcd for [C8H9BrN2OS]+[M+H]+: 261.0; found: 261.0

S-[(5-bromo-3-pyridyl)]N,N-dimethylcarbamothioate



[0390] 



[0391] O-[(5-bromo-3-pyridyl)] N,N-dimethylcarbamothioate (9.93 g, 0,04 mol) was dissolved in phenoxybenzene (100 mL). The mixture was heated at reflux for 2 h. The reaction mixture was cooled to room temperature and directly purified by flash chromatography on a Biotage (EtOAc/PE=5% ∼50%, ISCO 120 g, 50 mL/min, normal phase silica gel,uv 254) to afford the target compound S-[(5-bromo-3-pyridyl)] N,N-dimethylcarbamothioate (4.63 g, 43.76 % yield) as a yellow solid.
ESI-MS m/z calcd for [C8H9BrN2OS]+[M+H]+: 261.0; found: 261.0

3-bromo-5-methoxy-benzenethiol



[0392] 



[0393] S-(3-chloro-5-methoxy-phenyl) N,N-dimethylcarbamothioate (1.044 g, 4 mmol) and KOH (897.21 mg, 16 mmol) was taken up in in ethanol/water (40mL, 3/1). The reaction mixture was heated at reflux for 2h. LC-MS analysis indicated total consumption of the starting material. The mixture was concentrated followed by addition of 10% aq NaOH (30 mL). The reaction mixture was washed with ether (15 mL X 3). The aqueous layer was acidified with aq KHSO4 to adjust the pH ∼2, followed by extraction with EtOAc (20 mL X 5). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product, which was used for next step directly without further purification.
ESI-MS m/z calcd for [C5H4BrNS]- [M-H]-: 188.9; found: 188.0.

5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0394] 



[0395] NaH (82.99 mg, 3.47 mmol) was added to a solution of 5-bromopyridine-3-thiol (658.67 mg, 3.47 mmol) in DMF (10 mL) at 0 °C. The solution was stirred at rt for 30 min. Then 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (1.01 g, 2.89 mmol) was added. The reaction mixture was stirred at 50°C for 2 h followed by cooling to room temperature. Water (50 mL) was added and the reaction mixture was extracted with EtOAc (15 mL X 3). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product, which was purified by biotage (EtOAc/PE=5% ∼40%, ISCO 40 g, 30 mL/min, normal phase silica gel, uv 254) to afford the title compound (650 mg, 44.7 % yield) as a white solid.
ESI-MS m/z calcd for [C17H19BrN4O7S]+ [M+H] +: 503.0; found: 503.0

5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0396] 



[0397] To a solution of 5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (650 mg, 1.29 mmol) in DMF (15 mL) was added trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (442.22 mg, 194 mmol), iodocopper (73.78 mg, 0.39 mmol), CsF (294.26 mg, 1.94 mmol) and N,N-diethylethanamine (653.39 mg, 6.46 mmol). The reaction vessel was purged 3 times with nitrogen. The reaction mixture was stirred at room temperature for 2 h. The mixture was filtered and washed with EtOAc (50 mL), The filtrate was concentrated in vacuo to afford the title compound as crude product, which was used for next step directly without further purification.
ESI-MS m/z calcd for [C25H22BrF3N4O7S]+ [M+H] +: 658.0; found: 659.0.

i45) 3-Chloro-5-cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


O-3-chloro-5-cyanophenyl dimethylcarbamothioate



[0398] 



[0399] To a mixture of 3-chloro-5-hydroxy-benzonitrile (2.5 g, 16.28 mmol) in DMF (25 mL)) cooled to 0°C was added sodiumhydride (0.43 g, 17.91 mmol) followed by dimethylthiocarbamoyl chloride (2.21 g, 17.91 mmol). The reaction mixture was stirred at room temperature for a 20 h followed by addition of water (50 ml), followed by extraction with DCM (100 ml 2). The combined organics were washed with brine, dried over Na2SO4 and evaporated to dryness to obtain crude product. Purification on silica gel (PE/EtOAc=10/1) to gav the target compound 2.5 g (63.80%) as a white solid.

S-3-chloro-5-cyanophenyl dimethylcarbamothioate



[0400] 



[0401] A solution of O-(3-chloro-5-cyano-phenyl) N,N-dimethylcarbamothioate (2.50 g, 10.39 mmol) in phenyl ether (20 ml) was stirred at 280°C for 2 h. TLC analysis indicated the total consumption of the SM. The reaction mixture was cooled to room temperature and purified by silica gel chromatography to give product (2.20 g, 88%) as a solid.

3-chloro-5-mercaptobenzonitrile



[0402] 



[0403] To a solution of S-(3-chloro-5-cyano-phenyl) N,N-dimethylcarbamothioate (300 mg, 1.25 mmol) in methanol (15 mL) was added 2M NaOH (8 ml). The reaction was stirred at 70°C for 2 h. TLC analysis indicated the total consumption of the SM. Water (30 mL) and DCM (30 mL) were added. The phases where separated and the aqueous phase was further extrated with DCM (50 mL2). The combined organics were washed with brine, dried over Na2SO4 followed by removal of the solvents in vacuo. The crude material was immediately used in the next step.

3-Chloro-5-cyanophenyl-2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0404] 



[0405] To a solution of 3-chloro-5-sulfanyl-benzonitrile (212 mg, 3 mmol) in DMF (15 mL) was added NaH (22.99 mg, 1.00 mmol). After stirring for 0.5 h, 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (218.53 mg, 0.62 mmol) was added and the reaction mixture was held at 50°C with stirring for 2 h. TLC analysis indicated the total consumption of the SM. 10% citric acid aq. (30 mL) and DCM (30 mL) were added and the phases where separated. The aqueous phase was further extracted with DCM (50 mL2). The combined organic layers were washed with brine, dried over Na2SO4 and the solvents where removed in vacuo. The crude material was purified by flash column chromatography to give the target compound 120 mg (20%) as white oil.

3-Chloro-5-cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0406] 



[0407] To a solution of 3-azido-1,3-dideoxy-2,4,6-tri-O-acetyl-1-(3-chloro-5-cyanophenyl-thio)-α-D-galactopyranoside (120 mg, 0.25 mmol) in DMF (3 mL) were added TEA (125.73 mg, 1.24 mmol), Copper(I)Iodide (14.20 mg, 0.07 mmol), trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (113.46 mg, 0.50 mmol). The reaction mixture was stirred at 100°C for 2 h. Water (80 mL) and DCM (80 mL) were added and the phases where separated. The aqueous phase was extrated with DCM (10 mL2) and the combined organic phases were washed with water (20 mL) and brine (20 mL), dried over anhydrous sodium sulphate and removal of solvent in vacuo. The crude material was purified by column chromatography(PE/EtOAc=2/1) to obtain the title compound (100 mg, 45%).

i46) 3-chloro-4-cyanophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0408] 



[0409] 3-Chloro-4-cyanothiophenol 196 mg, 1.16 mmol) was dissolved in DMF (2 mL) followed by addition of NaH (58-62%, 50 mg, 1.30 mmol). The reaction mixture was stirred at r.t. for one hour followed by addition to a stirred solution of 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (280 mg, 0.80 mmol) in DMF (2 mL). The resulting mixture was heated to 55 °C for 100 minutes. EtOAc was added and the organics were washed four times with brine. The aqueous phase was extracted once with EtOAc and the combined organic phase was dried over Na2SO4 and the solvents where removed in vacuo. The residue was purified by flash chromatography (SiO2, 0-60% EtOAc in petroleum ether to give the title compound, 98 mg as a white solid. 1H NMR (400 MHz, Chloroform-d) δ 7.61 - 7.55 (m, 2H), 7.40 (dd, J = 8.3, 1.6 Hz, 1H), 6.15 (d, J = 5.5 Hz, 1H), 5.50 - 5.46 (m, 1H), 5.30 (dd, J = 11.0, 5.6 Hz, 1H), 4.55 - 4.50 (m, 1H), 4.13 (dd, J = 11.6, 4.9 Hz, 1H), 4.01 (dd, J = 11.6, 7.7 Hz, 1H), 3.94 (dd, J = 11.0, 3.2 Hz, 1H), 2.17 (s, 6H), 1.94 (s, 3H). ESI-MS m/z calcd for [C19H19CN4O7SNa]+ (M+Na)+: 505.1; found: 505.0.

i47) 3-Chloro-6-fluoro-4-cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


S-5-chloro-4-cyano-2-fluorophenyl O-ethyl carbonodithioate



[0410] 



[0411] 4-amino-2-chloro-5-fluoro-benzonitrile (2.0 g, 11.73 mmol) was dissolved in 20 ml HCl at 0°C. Followed by slow addition of a water solution of NaNO2 (810 mg, 11.73mmol). The reaction mixture was stirred at 0°C until the material was dissolved. The reaction mixture was filtered and slowly added to a 50°C solution of Potassium ethyl xanthogenate (5.64 g, 35.18 mmol) in water (15 mL). The reaction mixture was stirred at 70°C for 2 hours followed by extraction with EtOAc (100 mL3). The combined organics were washed with brine, dried over Na2SO4, filtered and concentrated in vacuo to give 3.0 g of crude material which was used in the next step. ESI-MS m/z calcd for [C10H8ClFNOS2]+ (M+H)+: 276.0; found: 276.0.

2-chloro-5-fluoro-4-mercaptobenzonitrile



[0412] 



[0413] O-ethyl (5-chloro-4-cyano-2-fluoro-phenyl)sulfanylmethanethioate (1.0 g, 3.63 mmol) was dissolved in 20 mL ethanol and heated to 85°C. KOH (0.41 g, 7.25 mmol) was added slowly followed by stirring at 85 °C for 2 hours. After cooling to room temperature the reaction mixture was acidified to pH=4 using HCl(1M) followed by extraction with ethylacetate EtOAc (50 mL3). The combined organic phases were washed with brine, dried over Na2SO4, filtered and concentrated in vacuo to give the title compound as a crude material, 1.0 g, which was used as is in the next step.

3-Chloro-6-fluoro-4-cyanophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0414] 



[0415] To a stirred solution of 2-chloro-5-fluoro-4-sulfanyl-benzonitrile (400 mg, 2.13 mmol) in DMF (5 ml), NaH (49 mg, 2.13 mmol) was added at 0°C. The reaction mixture was stirred at rt for 30 min followed by addition of 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (1.1 g, 3.41 mmol). The reaction mixture was stirred at 50 °C for 1 hour followed by removal of solvent in vacuo. The resulting crude was diluted with CH2Cl2 and washed with water and brine. The organic layer was dried over Na2SO4, filtered and the solvents where removed in vacuo. The crude product was purified by chromatography column (PE:EtOAc=3:1) to afford the titel compound (130 mg, as white solid).

3-Chloro-6-fluoro-4-cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0416] 



[0417] 3-Chloro-6-fluoro-4-cyanophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (130 mg, 0.26 mmol), trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (120 mg, 0.52 mmol), Copper(I)Iodide (15 mg, 0.08 mmo) and Triethylamine (131 mg, 1.3 mmol) were added to a flask. DMF (3 mL) was added. The mixture was stirred at 100 °C for 1 hour under N2 protection. Then the mixture was filtered and concentrated in vacuo. The residue was purified by flash chromatography to give title compound 160 mg. ESI-MS m/z calcd for [C27H22ClF4N4O7S]+ (M+H)+: 657.1; found: 657.1.

i48) 3-Bromo-4-cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


2-bromo-4-mercaptobenzonitrile



[0418] 



[0419] 2-bromo-4-fluoro-benzonitrile (2 g, 10 mmol) was dissolved in DMF (5 mL). Na2S•10H2O (2.58 g, 10 mmol) was added. The mixture was stirred at rt 1 h. Then 1M NaOH (100 mL) was added. The mixture was extracted by DCM (100 mL2). Then the water phase was acided by 6 M HCl to pH=2. The mixture was extracted by DCM (200 mL2). The organic phase was washed by brine, dried over Na2SO4, filtered and concentrated. The crude material was used for next step without further purification.

3-Bromo-4-cyanophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0420] 



[0421] NaH (60%, 168.72 mg, 4.4 mmol) and 2-bromo-4-sulfanyl-benzonitrile (1g crude) were added to a flask. DMF (5 mL) was added under N2 protection at 0 °C. The reaction mixture was stirred at 0 °C for 15 min. Then 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (700 mg, 2 mmol) dissolved in DMF (5 ml) was added to the reaction mixture. The reaction was stirred at rt for 2 hours. EtOAc (200 ml) was added. The mixture was washed by water (100 mL), brine (100 mL), dried over Na2SO4, filtered and concentrated in vacuo. The residue was purified on silica gel column to give the title compound 240 mg (22.7%).
ESI-MS m/z calcd for [C19H19BrN4NaO7S]+ (M+Na)+: 549.0; found: 549.0.

3-Bromo-4-cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0422] 



[0423] 2,4,6-tri-o-acetyl-1-(3-bromo-4-cyanophenylthio)-3-azido-1,3-dideoxy-α-D-galactopyranoside (240 mg, 0.46 mmol), 2-(3,5-difluoro-4-methoxy-phenyl)ethynyl-trimethyl-silane (328.12 mg, 1.37 mmol), Copper(I)Iodide (26 mg, 0.14 mmol) and triethylamine (230.26 mg, 2.28 mmol) was dissolved in DMF (5 mL). The mixture was stirred at 100°C for 1 hour. EtOAc (200 ml) was added. The mixture was filtered, washed by water (100 mL), dried by Na2SO4, filtered and concentrated. The residue was purified on silica gel column. 220 mg (70.7%) of the title compound was obtained.
ESI-MS m/z calcd for [C27H23BrF3N4O7S]+ (M+H)+: 683.0; found: 683.1.

5-Bromo-6-trifluoromethyl-pyridin-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0424] 



[0425] NaH (15.59 mg, 0.65 mmol) was added to a solution of 5-bromo-6-(trifluoromethyl)pyridine-3-thiol (140 mg, 0.54 mmol) in DMF (10 mL) at 0 °C. The solution was stirred at rt for 30 min. Then 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (189.73 mg, 0.54 mmol) was added to mixture. The reaction was stirred at 50°C for 2 h. The mixture was cooled to room temperature and water (20 mL) was added followed by extraction with EtOAc (15 mL X 3). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product, which was purified by flash chromatography on a biotage (EtOAc/PE=5% ∼ 40%, ISCO 20 g, 15 mL/min, normal phase silica gel,uv 254) to afford crude 3-azido-1,3-dideoxy-2,4,6-tri-O-acetyl-1-(3-chloro-2-trifluoromethylpyridine-5-yl-thio)-α-D-galactopyranoside (70 mg, 22.58 % yield).
ESI-MS m/z calcd for [C18H18BrF3N4O7S]+ [M+H] +: 570.0; found: 571.0.

5-Bromo-6-trifluoromethyl-pyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0426] 



[0427] 5-Bromo-6-trifluoromethyl-pyridin-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (70 mg, 0.12 mmol), trimethyl((3,4,5-trifluorophenyl)ethynyl)silane (27.97 mg, 0.12 mmol), CuI (7.0 mg, 0.04 mmol) and Et3N (18.60 mg, 0.18 mmol) were dissolved in DMF (10 mL) and the mixture was stirred at 50 °C for 2 h. Then the solvent was removed in vacuo to afford crude product (90 mg, >100% yield), which was used for next step without further purification.
ESI-MS m/z calcd for [C26H21BrF6N4O7S]+ (M+H)+: 726.0; found: 727.0.

i50) 5-Chloro-6-cyano-pyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside 3-chloro-5-mercaptopicolinonitrile



[0428] 



[0429] To a solution of 3,5-dichloropyridine-2-carbonitrile (50 mg, 0.29 mmol) in DMF (2 mL) was added Na2S (33.83 mg, 0.43 mmol). After completion of the reaction the mixture was added NaHSO4 (aq) to adjust pH 4-5 followed by DCM(10 mL). The organic phase was washed with water (10 mL) and brine (10 mL), dried over anhydrous sodium sulphate. Removal of solvent gave the desired product (30 mg, crude).
m/z calcd for [C6H3CN2S]-[M-H]-: 169.0; found: 169.0.

5-Chloro-6-cyano-pyridin-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0430] 



[0431] To a solution of 3-chloro-5-sulfanyl-pyridine-2-carbonitrile(30 mg, 0.17 mmol) in DMF (3 mL) was added NaH (3.94 mg, 0.17 mmol) at 0°C. After 10 min, 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (40 mg, 0.11mmol) was added. The reaction mixture was stirred at at room temperature for 4 h. Water (20 mL) and DCM (10 mL) were added. The aqueous phase was extracted with DCM (10 mL2), and the combined organic phase was washed with water (20 mL), brine (20 mL), dried over anhydrous sodium sulphate and the solvents were removed in vacuo. The crude material was purified by flash chromatography (Petroleum Ether/EtOAc=3/1) to obtain the title compoundt (20 mg, 36.14%).
m/z calcd for [C18H18ClN5O7S]+ [M+H]+: 484.0; found: 484.0.

5-Chloro-6-cyano-pyridin-3-yl-2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0432] 



[0433] To a solution of 3-azido-1,3-dideoxy-2,4,6-tri-O-acetyl-1-(3-chloro-2-cyanopyridine-5-yl-thio)-α-D-galactopyranoside (20 mg, 0.04 mmol) in DMF (3 mL) were added TEA (20.91 mg, 0.21 mmol), Copper(I)Iodide(2.36 mg, 0.01 mmol), CsF(9.42 mg, 0.06 mmol), trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane(14.15 mg, 0.06 mmol). The reaction was stirred at room temperature for 2 h under N2. Water (80 mL) and DCM (80 mL) were added. The aqueous phase was extrated with DCM (10 mL2), the combined organic phase were washed with water (20 mL) and brine (20 mL), dried over anhydrous sodium sulphate followed by removbal of the solvent in vacuo. The crude material was purified by column chromatography(Petroleum ether/Ethyl acetate =2/1) to obtain the title compound (12 mg, 45.36%).m/z calcd for [C26H21ClF3N5O7S]+ [M+H]+: 640.0; found: 640.0.

i51) 5-Hydroxy-6-cyano-pyridazin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


Diethyl 2-diazo-3-oxopentanedioate



[0434] 



[0435] To a mixture of diethyl 3-oxopentanedioate (5 g, 24.73 mmol) and TEA (3.8 ml) in CH3CN (100 ml) at 0°C was added N-(4-azidosulfonylphenyl)acetamide (5.9 g, 24.73 mmol) in several portions. The reaction was stirred at room temperature for 1h followed by filtration. The filter was washed with 1:1 hexane: ether and the filtrate was stirred on an ice bath for 30 min upon formation of additional precipitate. This was filtered off and the filter and washed with 1:1 hexane:ether. The filtrates where combined and the solvents where removed in vacuo to give the title compound as a yellow oil. (5.5 g, 97.47%)
m/z calcd for [C9H12N2O5]+[M-N2]+: 200.0; found: 200.0.

ethyl 4,6-dihydroxypyridazine-3-carboxylate



[0436] 



[0437] A mixture of diethyl 2-diazo-3-oxo-pentanedioate(1 g, 4.38 mmol) and PPh3 (1.15 g, 4.38 mmol) in ether (50 ml) was stirred at room temperature for 48 h. Ether was removed in vacuo and HOAc (50 ml) and water (5 ml) was added to the residue. The mixture was refluxed for 10 h under N2. The solvent was removed in vacuo to dryness. The residue was purified by column chromatography(DCM/MEOH=10/1) to obtain the desired product (2.1 g, 47%).
m/z calcd for [C7H8N2O4]+ [M+H]+: 185.0; found: 185.0.

4,6-dihydroxypyridazine-3-carboxamide



[0438] 



[0439] To a solution of ethyl 4,6-dihydroxypyridazine-3-carboxylate (2.1 g, 11.40 mmol) in 40 ml NH3-CH3OH was held at room temperature with stirring on for 20h under N2. The solvents were removed in vacuo, and the residue was used to next step directly.
m/z calcd for[C5H5N3O3]+ [M+H]+: 156.0; found: 156.0.

4,6-dichloropyridazine-3-carbonitrile



[0440] 



[0441] To a solution of 4,6-dihydroxypyridazine-3-carboxamide (800 mg, 5.16 mmol) in POCl3 (10 mL) was stirred at 100°C for 3 h under N2. The reaction mixture was poured into ice water and extracted with DCM (40 ml2). The combined organic phase was washed with brine (50 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The residue was purified by column chromatography(PE/EtOAc =10/1) to obtain 4,6-dichloropyridazine-3-carbonitrile (280mg).
m/z calcd for[C5HCl2N3]+ [M+H]+: 174.0; found: 174.0.

1,2,4,6-tetra-O-acetyl-1-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-β-D-galactopyranoside



[0442] 



[0443] To a solution of 1,2,4,6-tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (2.00 g, 5.36 mmol) in DMF (20 mL) were added TEA (2.71 g, 26.80 mmol), Copper(I)Iodide (306.16 mg, 1.61 mmol), trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane(1.83 g, 8.04 mmol). The reaction was held at 100°C with stirring on for 2 h under N2. Water (80 mL) and DCM (80 mL) were added. The aqueous phase was extrated with DCM (10 mL2), the combined organic phase was washed with water (20 mL) and brine (20 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The residue was purified by column chromatography(PE/EtOAc=2/1) to obtain title compound (2 g, 71%).

2,4,6-tri-O-acetyl-1-chloro-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-β-D-galactopyranoside



[0444] 



[0445] To a stirred suspension of 1,2,4,6-tetra-O-acetyl-1-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-β-D-galactopyranoside (2.00 g, 3.78 mmol) and PCl5 (1.56 g, 7.56 mmol) in dry DCM (20 mL), BF3·Et2O (2 mL) was added. After stirring for 2 h, TLC analysis showed complete disappearance of the starting material. The reaction mixture was diluted with DCM (50 mL) and then washed with ice-cold water, sat. ice-cold NaHCO3 solution (2×50 mL), and again ice-cold water successively, dried over anhydrous Na2SO4 and evaporated under reduced pressure to afford the crude product 1 g as a white solid. Which was used directly in the next step.

Acetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0446] 



[0447] To a solution of 2,4,6-tri-O-acetyl-1-chloro-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-β-D-galactopyranoside (1.00 g, 1.98 mmol) in DMF (10 mL) were added Potassium thioacetate (452.31 mg, 3.96 mmol). The reaction mixture was stirred at room temperature for 20 h. Water (40 mL) and DCM (40 mL) were added. The aqueous phase was extrated with DCM (40 mL2), and the combined organic phase was washed with water (50 mL) and brine (50 mL), dried over anhydrous sodium sulphate and evaporated to dryness. The crude products were purified by flash column chromatography (PE/EtOAc=3/2) to obtain title compound (450 mg, 42%).

2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0448] 



[0449] To a solution of Acetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (200 mg, 0.36 mmol) in DCM/methanol (30 mL) was cooled to 0 °C under N2. Then sodium thiomethoxide (25.68 mg, 0.36 mmol) were added. The reaction mixture was stirred at 0°C for 30 min. The pH was adjusted to 5-6 with d 0.3M HCl aq. The organic layer was washed with water (60 mL) and brine (60 mL), dried over anhydrous sodium sulphate. Removal of solvent gave the crude product (160 mg).
m/z calcd for [C20H20F3N3O7S]+ [M+H]+: 504.0; found: 504.0.

5-Hydroxy-6-cyano-pyridazin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0450] 



[0451] To a solution of 1,3-dideoxy-2,4,6-tri-O-acetyl-1-mercapto-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside (160 mg, 0.32 mmol) in DMF (20 mL) was added 4,6-dichloropyridazine-3-carbonitrile (82.96 mg, 0.48 mmol) at ice bath under N2. Then NaH(10.96 mg, 0.48 mmol) was added. The mixture was held at room temperature with stirring for 4 h. Water (40 mL) and DCM (20 mL) were added. The aqueous phase was extrated with DCM (20 mL2), the combined organic phase was washed with water (40 mL) and brine (40 mL), dried over anhydrous sodium sulphate and the solvents were removed in vacuo. The crude material was purified by purified by column chromatography(PE/EtOAc=2/1) to obtain the desired product (42 mg, 20%).
m/z calcd for [C25H20ClF3N6O7S]+ [M+H]+: 641.0; found: 641.0.

Example 56


3-Chloro-2,4-difluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0452] 



[0453] 3-Chloro-2,4-difluoro-benzenethiol (110 mg) was dissolved in DMF (1 mL). Sodium hydride (54 mg, ca 60% in mineral oil) was added followed by 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranosid (135 mg) dissolved in DMF (2 mL).

[0454] The mixture was heated to 50 °C 1 h and then stirred at r.t. over night. It was partitioned between Et2O (100 mL) and water (3x150 mL). The organic phase was purified by flash chromatography (SiO2, 5-95% EtOAc in heptane). This afforded the intermediate thioglycoside as an oil (96 mg). Copper(I) iodide (10 mg), 3,4,5-triflourophenylacetylene (0.080 mL) and DIEA (0.200 mL) were added and the resulting mixture stirred 2.5 h at r.t. The mixture was concentrated in vacuo. The residue was dissolved/suspended in EtOAc (5 mL) and filtered through a short column of silica. The solvent was removed in vacuo and the residue was dissolved in MeOH (20 mL). 1M sodium methoxide in MeOH (2 mL) was added and the mixture left at r.t. over night. The mixture was concentrated in vacuo and the residue was dissolved in small volume MeCN/water, filtered and purified by HPLC (C18/H2O:MeCN:0.1% TFA). Freezedrying afforded the product as a white powder (63 mg). 1H NMR (400 MHz, Methanol-d4) δ 8.54 (s, 1H), 7.74 - 7.58 (m, 3H), 7.14 (td, J = 8.7, 1.8 Hz, 1H), 5.88 (d, J = 5.4 Hz, 1H), 5.07 (dd, J = 11.3, 2.8 Hz, 1H), 4.92 (dd, J = 11.3, 5.4 Hz, 1H), 4.44 (t, J = 6.2 Hz, 1H), 4.22 (d, J = 2.8 Hz, 1H), 3.66 (dd, J = 11.4, 5.9 Hz, 1H), 3.55 (dd, J= 11.4, 6.4 Hz, 1H). ESI-MS m/z calcd for [C20H16ClF5N3O4S]+ (M+H)+: 524.04; found: 524.00.

Example 57


3,4-Dichlorophenyl 3-deoxy -3-[4-(phenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0455] 



[0456] To a stirred solution of 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(phenyl)-1-thio-α-D-galactopyranoside (100 mg) in MeOH (5 mL), NaOMe (9 mg) was added. The mixture was stirred at r.t. for 2 hours. Then the mixture was purified by reverse-phase chromatography to give 40 mg (50.8%) of 3,4-Dichlorophenyl 3-deoxy-3-[4-(phenyl )-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside.
1H NMR (400 MHz, DMSO) δ 8.58 (s, 1H), 7.92 - 7.86 (m, 2H), 7.84 (d, J = 2.1 Hz, 1H), 7.60 (d, J = 8.4 Hz, 1H), 7.53 (dd, J = 8.4, 2.1 Hz, 1H), 7.45 (t, J = 7.7 Hz, 2H), 7.33 (t, J = 7.4 Hz, 1H), 5.89 (dd, J = 19.3, 4.3 Hz, 2H), 5.48 (d, J = 6.5 Hz, 1H), 4.87 - 4.75 (m, 2H), 4.71 (t, J = 5.6 Hz, 1H), 4.25 (t, J = 6.1 Hz, 1H), 4.04 (d, J = 6.4 Hz, 1H), 3.60 - 3.50 (m, 1H), 3.48 - 3.37 (m, 1H). ESI-MS m/z calcd for [C20H20Cl2N3O4S]+ (M+H)+: 468.1; found: 468.0.

Example 58


3,5-Dichloro-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0457] 



[0458] 3,5-Dichloro-4-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (50 mg, 0.1 mmol), trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (43.87 mg, 0.2 mmol), Copper(I)Iodide (6 mg, 0.03 mmo) and triethylamine (49 mg, 0.48 mmol) were added to a flask. DMF (2.5 mL) was added. The mixture was stirred at 50°C for 1 h under N2 protection. LCMS showed that the target product was formed and no SM left. The mixture was filtered and concentrated in vacuum to afford crude intermediate. This was dissolved in MeOH (5 mL) and cooled to 0°C followed by addition of sodium methoxide (5.0 mg, 0.09 mmol). The mixture was stirred at room temperature for 30 min. LCMS showed that the target product was formed and no SM left. Then the mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered and concentrated in vacuo to afford crude product, which was purified by Prep-HPLC to afford give the title compound (40 mg).
1H NMR (400 MHz, CDCl3) δ 8.12 (s, 1H), 7.53 (d, J = 6.1 Hz, 2H), 7.49 - 7.40 (m, 2H), 5.83 (d, J = 5.3 Hz, 1H), 4.97 - 4.86 (m, 1H), 4.74 - 4.67 (m, 1H), 4.66 - 4.59 (m, 1H), 4.49 - 4.42 (m, 1H), 4.42 - 4.31 (m, 1H), 4.06 (tt, J = 12.2, 5.9 Hz, 2H). ESI-MS m/z calcd for [C20H15Cl2F4N3O4S]+ (M+H)+: 539.0; found: 540.0.

Example 59


3,4-Dichloro-6-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0459] 



[0460] Sodium methoxide (4 mg, 0.07 mmol) was added to a stirred solution of 3,4-Dichloro-6-fluorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (150 mg, 0.23mmol) in MeOH (5 mL) at 0°C. The mixture was stirred at room temperature for 1h. LCMS showed no SM. The mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered and concentrated in vacuo to afford crude product, which was purified by preparative HPLC to afford the title compound (50 mg).
1H NMR (400 MHz, DMSO) δ 8.82 (s, 1H), 7.99 (d, J = 7.1 Hz, 1H), 7.97 - 7.82 (m, 2H), 7.77 (d, J = 9.0 Hz, 1H), 6.14 - 5.96 (m, 2H), 5.53 (d, J = 6.3 Hz, 1H), 4.89 (dd, J = 11.3, 2.7 Hz, 1H), 4.81 - 4.72 (m, 1H), 4.67 (t, J = 5.5 Hz, 1H), 4.18 (t, J = 6.3 Hz, 1H), 4.07 - 4.00 (m, 1H), 3.55 - 3.42 (m, 1H), 3.42 - 3.34 (m, 1H) ESI-MS m/z calcd for [C20H15Cl2F4N3O4S]+ (M+H)+: 539.0; found: 540.0.

Example 60


3-Bromo-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0461] 



[0462] To a solution of 3-Bromo-4-fluorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (250 mg, 0.37 mmol) in methanol (10 mL) were added sodium methoxide (6.79 mg, 0.13 mol). The reaction was held at room temperature with stirring on for 2 h. The reaction mixture was evaporated to dryness. The crude product was purified by preparative HPLC to afford 3-Bromo-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside
(40 mg, 36.15%) as a white solid.
1H NMR (400 MHz, DMSO) δ 8.80 (s, 1H), 8.10 - 7.73 (m, 3H), 7.72 - 7.54 (m, 1H), 7.38 (t, J = 8.7 Hz, 1H), 5.99 - 5.71 (m, 2H), 5.52 (d, J = 6.3 Hz, 1H), 4.84 - 4.69 (m, 2H), 4.30 (t, J = 6.0 Hz, 1H), 4.03 (d, J = 4.2 Hz, 1H), 3.63 - 3.50 (m, 1H), 3.50 - 3.38 (m, 1H).
m/z calcd for [C20H16BrF4N3O4S]+ [M+H]+: 550.0; found: 550.0.

Example 61


3-Chloro-4-(trifluoromethyl)phenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0463] 



[0464] 3-Chloro-4-(trifluoromethyl)phenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (32 mg) was dissolved in MeCN (3 mL) and stirred at r.t. under argon. Copper(I) iodide (20 mg) was added and after five minutes 1,2,3-trifluoro-5-[2-(trimethylsilyl)ethynyl]benzen (0.026 mL) was added. After an additional five minutes DIEA (0.011 mL) was added and the mixture was heated to 70 °C. After 5 h the reaction mixture was filtered through a short column of silica, eluting with EtOAc and then concentrated in vacuo. The crude product was dissolved in methanolic NaOMe (0.05M, 10 mL) and stirred at r.t. After 18 h acetic acid (2 mL) was added and the mixture was concentrated in vacuo. The crude product was purified by preparative HPLC (C18/H2O:MeCN:0.1% TFA) and lyophilized to give 10 mg of 3-chloro-4-(trifluoromethyl)phenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside. 1H NMR (400 MHz, Methanol-d4) δ 8.55 (s, 1H), 7.84 (s, 1H), 7.74 - 7.61 (m, 4H), 6.05 (d, J = 5.0 Hz, 1H), 4.98 (qd, J = 11.4, 3.7 Hz, 2H), 4.43 (t, J = 6.1 Hz, 1H), 4.20 (s, 1H), 3.71 (tt, J= 11.4, 6.3 Hz, 2H). ESI-MS m/z calcd for [C21H17ClF3N3O4S]+ (M+H)+: 556.1; found: 556.0.

Example 62


3,4,5-Trichlorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0465] 



[0466] 3,4,5-Trichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (64 mg) was dissolved in MeCN (5 mL) and stirred at r.t. under argon. Copper(I) iodide (18 mg) was added and after five minutes 1,2,3-trifluoro-5-[2-(trimethylsilyl)ethynyl]benzen (0.051 mL) was added. After an additional five minutes DIEA (0.022 mL) was added and the mixture was heated to 70 °C. After 2 h 1,2,3-trifluoro-5-[2-(trimethylsilyl)ethynyl]benzen (0.051 mL) was added and the temperature decreased to 50 °C. After 16 h the reaction mixture was filtered through a short column of silica, eluting with EtOAc and then concentrated in vacuo. The crude product was dissolved in methanolic NaOMe (0.05M, 20 mL) and stirred at r.t. After 2.5 h acetic acid (2 mL) was added and the mixture was concentrated in vacuo. The crude product was purified by preparative HPLC (C18/H2O:MeCN:0.1% TFA) and lyophilized to give 43 mg (64%) of 3,4,5-trichlorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside. 1H NMR (400 MHz, Methanol-d4) 8.54 (s, 1H), 7.76 (s, 2H), 7.69 - 7.60 (m, 2H), 5.92 (d, J = 4.9 Hz, 1H), 5.03 - 4.89 (m, 2H), 4.46 (t, J = 6.0 Hz, 1H), 4.20 (s, 1H), 3.79 - 3.65 (m, 2H). ESI-MS m/z calcd for [C20H16Cl3F3N3O4S]+ (M+H)+: 556.0; found: 556.0.

Example 63


5-Chloro-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0467] 



[0468] 5-Chloro-2-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (87 mg) was dissolved in MeCN (6 mL) and stirred at r.t. under N2. Copper(I) iodide (13 mg) was added and after five minutes 1,2,3-trifluoro-5-[2-(trimethylsilyl)ethynyl]benzen (0.080 mL) was added. After an additional five minutes DIEA (0.035 mL) was added and the mixture was stirred at r.t. After 18 h the mixture was heated to 70 °C. After 6 h the reaction mixture was filtered through a short column of silica, eluting with EtOAc and then concentrated in vacuo. The crude product was dissolved in methanolic NaOMe (0.05M, 20 mL) and stirred at r.t. After 18 h acetic acid (2 mL) was added and the mixture was concentrated in vacuo. The crude product was purified by preparative HPLC (C18/H2O:MeCN:0.1% TFA) and lyophilized to give 71 mg of 5-chloro-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside. 1H NMR (400 MHz, Methanol-d4) 8.55 (s, 1H), 7.73 (d, J = 6.1 Hz, 1H), 7.65 (t, J = 7.2 Hz, 2H), 7.39 - 7.29 (m, 1H), 7.15 (t, J = 8.8 Hz, 1H), 5.95 (d, J = 5.3 Hz, 1H), 5.06 (d, J = 11.4 Hz, 1H), 4.94 (dd, J = 11.2, 5.2 Hz, 1H), 4.44 (t, J = 6.0 Hz, 1H), 4.23 (s, 1H), 3.68 (dd, J = 11.1, 6.2 Hz, 1H), 3.57 (dd, J = 11.2, 6.2 Hz, 1H). 13C NMR (101 MHz, Methanol-d4) δ 163.6, 154.1, 151.7, 141.7, 139.3, 135.3, 130.6, 130.5, 123.6, 123.0, 118.1, 117.8, 110.9, 110.7, 89.6, 73.2, 69.5, 66.7, 65.4, 61.8. ESI-MS m/z calcd for [C20H17ClF4N3O4S]+ (M+H)+: 506.1; found: 506.0.

Example 64


5-Bromo-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0469] 



[0470] To a solution of 5-Bromo-2-fluorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (170 mg, 0.25 mol) in methanol (10 mL) were added sodium methoxide (6.79 mg, 0.13 mol). The reaction was held at room temperature with stirring on for 2 h. The reaction mixture was evaporated to dryness. The crude product was purified by preparative HPLC to afford 5-Bromo-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside (50 mg, 36.15%) as a white solid.
1H NMR (400 MHz, MeOD) δ 8.57 (s, 1H), 7.89 (dd, J = 6.4, 2.4 Hz, 1H), 7.72 - 7.63 (m, 2H), 7.58 - 7.46 (m, 1H), 7.12 (t, J = 8.9 Hz, 1H), 5.95 (d, J = 5.4 Hz, 1H), 5.16 - 5.02 (m, 1H), 4.98 - 4.93 (m, 1H), 4.46 (t, J = 6.1 Hz, 1H), 4.23 (d, J= 2.0 Hz, 1H), 3.79 - 3.64 (m, 1H), 3.64 - 3.54 (m, 1H).
m/z calcd for [C20H16BrF4N3O4S]+ [M+H]+: 550.0; found: 550.0.

Example 65


5-Chloro-2-methoxyphenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0471] 



[0472] 5-Chloro-2-methoxyphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (59 mg) was dissolved in MeCN (5 mL) and stirred at r.t. under N2. Copper(I) iodide (13 mg) was added and after five minutes 3,4,5-trifluorophenylacetylene (0.040 mL) was added. After an additional five minutes DIEA (0.022 mL) was added and the mixture was stirred at r.t. After 18 h the reaction mixture was filtered through a short column of silica, eluting with EtOAc and then concentrated in vacuo. The crude product was dissolved in methanolic NaOMe (0.05M, 20 mL) and stirred at r.t. After 5.5 h acetic acid (2 mL) was added and the mixture was concentrated in vacuo. The crude product was purified by preparative HPLC (C18/H2O:MeCN:0.1% TFA) and lyophilized to give 31 mg of 5-chloro-2-methoxyphenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside. 1H NMR (400 MHz, Methanol-d4) δ 8.54 (s, 1H), 7.70 - 7.58 (m, 3H), 7.27 (dd, J = 8.8, 2.5 Hz, 1H), 6.98 (d, J = 8.8 Hz, 1H), 5.96 (d, J = 5.4 Hz, 1H), 5.07 (dd, J = 11.3, 2.8 Hz, 1H), 4.92 (dd, J = 11.4, 5.4 Hz, 1H), 4.45 (t, J = 6.2 Hz, 1H), 4.22 (d, J = 2.1 Hz, 1H), 3.89 (s, 3H), 3.68 (dd, J = 11.1, 6.6 Hz, 1H), 3.54 (dd, J = 11.2, 6.0 Hz, 1H). 13C NMR (101 MHz, Methanol-d4) δ 159.1, 133.8, 129.4, 126.7, 124.6, 123.0, 113.2, 110.9, 110.7, 88.4, 72.9, 69.5, 66.8, 65.5, 61.7, 56.7. ESI-MS m/z calcd for [C21H20ClF3N3O5S]+ (M+H)+: 518.1; found: 518.0.

Example 66


3-Iodophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0473] 



[0474] 3-Iodophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (50 mg) was dissolved in MeCN (10 mL). Copper(I) iodide (5 mg) and 3,4,5-trifluorophenylacetylene (0.100 mL) were added and the mixture degassed (N2). Cesium fluoride (21 mg) was added and after 5 min, DIEA (0.200 mL) was added and the resulting mixture stirred 4 h. It was then filtered through a small plug of silica and concentrated in vacuo. The residue was suspended in MeOH (40 mL). 1M sodium methoxide in MeOH (1 mL) was added. After stirring at r.t. 2 h, acetic acid (1 mL) was added and the mixture concentrated in vacuo. The residue was dissolved in a small volume MeCN/water, filtered and purified by HPLC (C18/H2O:MeCN:0.1% TFA). Freezedrying afforded the title compound as a white powder (38 mg). 1H NMR (400 MHz, Methanol-d4) δ 8.54 (s, 1H), 7.98 (t, J = 1.6 Hz, 1H), 7.71 - 7.58 (m, 3H), 7.10 (t, J = 7.9 Hz, 1H), 5.80 (d, J = 5.2 Hz, 1H), 4.98 (dd, J = 11.4, 2.8 Hz, 1H), 4.91 (dd, J = 11.4,5.1 Hz, 1H), 4.52 (t, J = 6.2 Hz, 1H), 4.21 (d, J = 2.7 Hz, 1H), 3.72 (qd, J = 11.4, 6.1 Hz, 2H). ESI-MS m/z calcd for [C20H18F3IN3O4S]+ (M+H)+: 579.99; found: 579.95.

Example 67


Picolinamide-5-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0475] 



[0476] To a solution of 2-cyanopyridine-5-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (1.2 g, 1.98 mmol) in i-MeOH/Et3N/H2O (10/3/3)(20 mL) was held at room temperature with stirring on for 4 d. The mixture was evaporated to dryness. And the residue was triturated with ether and filtered. The reaction mixture was purified by preparative HPLC to give Picolinamide-5-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (20 mg, 1.87%).
m/z calcd for [C20H18F3N5O5S]+ [M+H]+: 498.0; found: 498.0.
1H NMR (400 MHz, MeOD) δ 8.78 (d, J = 1.8 Hz, 1H), 8.59 (s, 1H), 8.21 (dd, J = 8.2, 2.2 Hz, 1H), 8.07 (d, J = 8.3 Hz, 1H), 7.68 (dd, J = 8.8, 6.7 Hz, 2H), 6.02 (d, J = 5.2 Hz, 1H), 5.06 (dd, J = 11.4, 2.7 Hz, 1H), 4.97 (dd, J= 11.4, 5.3 Hz, 1H), 4.49 (dt, J= 6.0 Hz, 1H), 4.22 (d, 1H), 3.77 - 3.66 (m, 2H).

Example 68


3-Cyanophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0477] 



[0478] 3-Cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside (72 mg) was dissolved in methanolic NaOMe (0.05M, 10 mL) and stirred at r.t. After 2 h acetic acid (2 mL) was added and the mixture was concentrated in vacuo. The crude product was purified by preparative HPLC (C18/H2O:MeCN:0.1% TFA) then (Xterra/25 mM NH3 in H2O:MeCN) and lyophilized to give 15 mg of 3-cyanophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside.
1H NMR (400 MHz, Methanol-d4) δ 8.55 (s, 1H), 7.99 (s, 1H), 7.91 (d, J= 8.0 Hz, 1H), 7.70-7.60 (m, 3H), 7.51 (t, J = 7.9 Hz, 1H), 5.91 (d, J = 5.2 Hz, 1H), 5.01 (dd, J= 11.4, 2.5 Hz, 1H), 4.93 (dd, J= 11.4, 5.2 Hz, 1H), 4.50 (t, J= 6.0 Hz, 1H), 4.20 (s, 1H), 3.8-3.3(m, 2H)
ESI-MS m/z calcd for [C21H17F3N4O4S]+ (M+H)+: 479.1; found: 479.0.

Example 69


2-cyanopyridine-5-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0479] 



[0480] To a solution of 2-cyanopyridine-5-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (1.2 g, 1.98 mmol) in i-PrOH/Et3N/H2O (10/3/3)(20 mL) was stirred at room temperature for 4 days. The reaction mixture was evaporated to dryness. The residue was triturated with ether and filtered to afford a white solid 2-cyanopyridine-5-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (600 mg, 63.26%). m/z calcd for [C20H16F3N5O4S]+ [M+H]+: 480.0; found: 480.0.
1H NMR (400 MHz, MeOD) δ 8.83 (d, J = 1.9 Hz, 1H), 8.58 (s, 1H), 8.22 (dd, J = 8.2, 2.3 Hz, 1H), 7.81 (d, J= 8.2 Hz, 1H), 7.67 (dt, J= 11.4, 5.7 Hz, 2H), 6.15 (d, J = 5.1 Hz, 1H), 5.07 (dd, J = 11.4, 2.7 Hz, 1H), 4.99 (dd, J = 11.4, 5.2 Hz, 1H), 4.40 (t, J = 6.0 Hz, 1H), 4.21 (d, J= 1.8 Hz, 1H), 3.75 - 3.65 (m, 2H).

Example 70


4-Chloro-2-thienyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0481] 



[0482] Sodium methoxide (0.53 mg, 0.01 mmol) was added to a solution of crude 4-chloro-2-thienyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (61 mg, 0.10 mmol) in MeOH (5.0 mL) at room temperature. The mixture was stirred at room temperature for 1 h. The mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered, washed with MeOH (50 mL) and concentrated in vacuo to afford crude product, which was purified by preparative-HPLC to afford 4-chloro-2-thienyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (6.64 mg, 13.7% yield).
1H NMR (400 MHz, MeOD) δ 8.56 (s, 1H), 7.67 (dd, J= 8.9, 6.6 Hz, 2H), 7.41 (d, J = 1.2 Hz, 1H), 7.27 (d, J = 1.2 Hz, 1H), 5.65 (d, J = 5.2 Hz, 1H), 4.98 (dt, J = 13.6, 6.8 Hz, 2H), 4.94 - 4.91 (m, 2H), 4.55 (t, J = 5.9 Hz, 1H), 4.24 (d, J = 1.6 Hz, 1H), 3.77 (qd, J = 11.3, 6.2 Hz, 2H).
ESI-MS m/z calcd for [C18H15ClF3N3O4S2]+ [M+H]+: 493.0; found: 494.0.

Example 71


3-Carboxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0483] 



[0484] To a solution of (methyl 1-benzoate)-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (30 mg, 0.05 mmol) in NH4OH (2 mL) was held at room temperature with stirring on for 20 h. The crude product was purified by pre-HPLC to obtain 3-Carboxyphenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (10 mg, 42.7%).
1H NMR (400 MHz, MeOD) δ 8.46 (s, 1H), 8.13 (s, 1H), 7.85 (d, J = 7.7 Hz, 1H), 7.76 (d, J= 7.5 Hz, 1H), 7.70 - 7.46 (m, 2H), 7.36 (t, J= 7.7 Hz, 1H), 5.75 (d, J= 5.3 Hz, 1H), 4.95 - 4.82 (m, 2H), 4.45 (t, J = 6.3 Hz, 1H), 4.13 (s, 1H), 3.83 - 3.45 (m, 2H).
m/z calcd for [C21H18F3N3O6S]+ [M+H]+: 498.0; found: 498.0.

Example 72


Benzamide-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0485] 



[0486] To a solution of 3-Carboxyphenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (4.00 mg, 0.01 mmol) in DMF (1 mL) were added NH4Cl (4.30 mg, 0.08 mmol), HATU (6.11 mg, 0.02 mmol), TEA (8.14 mg, 0.08 mmol). The reaction mixture was held at room temperature with stirring on for 20 h. The crude prouct was purified by pre-HPLC to obtain Benzamide-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside
(1.42 mg, 35.57%).
1H NMR (400 MHz, MeOD) δ 8.46 (s, 1H), 8.02 (s, 1H), 7.70 (d, J = 7.9 Hz, 2H), 7.67 (t, J = 22.8 Hz, 2H), 7.56 (dd, J = 8.7, 6.8 Hz, 1H), 7.56 (dd, J = 8.7, 6.8 Hz, 1H), 7.35 (t, J = 7.8 Hz, 1H), 5.75 (d, J = 5.2 Hz, 1H), 4.92 (dd, J = 11.4, 2.8 Hz, 1H), 4.83 (d, J= 5.3 Hz, 1H), 4.48 (t, J= 5.9 Hz, 1H), 4.11 (d, J= 1.9 Hz, 1H), 3.77 - 3.52 (m, 2H).
m/z calcd for [C21H19F3N4O5S]+ [M+H]+: 497.0; found: 497.0.

Example 73


3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0487] 



[0488] To a solution of 3,4-dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (400 mg, 0.63 mmol) in methanol (10 ml). Sodium methanolate solution (30%, 5 mg) was added and the solution was stirred at room temperature for 2 h. LC-MS analysis indicated the total consumption of the starting material. After completion, DOWEX 50wx8-200 Ion exchange resin was added(PH=7) and the reactionmixture was filtered. The filtrate was concentrated purified by combiflash (EtOAc:PE:=1:20 to 1:5 ISCO 40 g 40 ml/min normal phase sillica,uv254) to afford the target compound 55 mg as a white solid.
1H NMR (500 MHz, DMSO) δ 7.99-7.95 (m, 1H), 7.85 (s, 1H), 7.79-7.75 (m, 1H), 7.60 (d, J= 8.4 Hz, 1H), 7.54-7.52 (m, 2H), 5.94 (dd, J= 20.4, 3.5 Hz, 2H), 5.50 (d, J = 6.7 Hz, 1H), 4.81-4.75 (m, 3H), 4.27 (t, J = 5.9 Hz, 1H), 4.06 (d, J = 5.5 Hz, 1H), 3.50-3.48 (m, 1H), 3.37-3.33 (m, 1H).
ESI-MS m/z calcd for [C20H17Cl2F2N3O4S]+ [M+H]+: 504.0; found: 504.0.

Example 74


3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0489] 



[0490] To a stirred solution of 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (160 mg, 0.25 mmol) in MeOH (5 mL), Sodium methoxide (4 mg, 0.07 mmol) was added at 0 °C. The mixture was stirred at rt for 0.5 hours. LCMS showed that the target product was formed and no SM left. The mixture was concentrated and the residue was purified by Prep-HPLC to give 50 mg of the title compound.
1H NMR (400 MHz, DMSO) δ 8.83 (s, 1H), 7.84 (d, J = 2.1 Hz, 1H), 7.66 - 7.59 (m, 3H), 7.53 (dd, J = 8.4, 2.1 Hz, 1H), 7.21 (t, J = 9.4 Hz, 1H), 5.91 (dd, J = 13.4, 4.6 Hz, 2H), 5.52 (d, J = 6.3 Hz, 1H), 4.88 - 4.67 (m, 3H), 4.25 (t, J = 6.3 Hz, 1H), 4.03 (d, J= 4.8 Hz, 1H), 3.58 - 3.51 (m, 1H), 3.47 - 3.38 (m, 2H).
ESI-MS m/z calcd for [C20H18Cl2F2N3O4S]+ (M+H)+: 504.1; found: 504.0.

Example 75


3,4-Dichlorophenyl 3-deoxy-3-[4-(3-fluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0491] 



[0492] To a stirred solution of 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3-fluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (140 mg) in MeOH (5 mL), NaOMe (12.35 mg) was added. The mixture was stirred at rt for 2 hours. Then the mixture was purified by reverse-phase chromatography to give 50 mg (45%) of the title compound.
1H NMR (400 MHz, DMSO) δ 8.72 (s, 1H), 7.85 (d, J= 1.8 Hz, 1H), 7.83 - 7.66 (m, 2H), 7.60 (d, J= 8.4 Hz, 1H), 7.59 - 7.47 (m, 2H), 7.16 (td, J= 8.4, 2.1 Hz, 1H), 6.04 - 5.82 (m, 2H), 5.50 (d, J = 6.4 Hz, 1H), 4.80 (s, 2H), 4.72 (t, J = 5.6 Hz, 1H), 4.25 (t, J= 6.2 Hz, 1H), 4.03 (d, J= 6.6 Hz, 1H), 3.64 - 3.50 (m, 1H), 3.46 - 3.38 (m, 1H).
ESI-MS m/z calcd for [C20H19Cl2FN3O4S]+ (M+H)+: 486.1; found: 486.2.

Example 76


3,3'-difluoro-cyklohexyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0493] 



[0494] To a solution of 3,3'-difluoro-cyklohexyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (10.00 mg, 0.01 mmol) in methanol (0.5 mL) and water (0.1mL) were added TEA (0.3 mL). The reaction was evaporated to dryness and the residue was purified by pre-HPLC to obtain the title compound (1 mg).
1H NMR (400 MHz, DMSO-d6) δ 8.53 (s, 1H), 7.66(dd, J =8.8, 6.4 Hz, 2H), 5.72(d, J = 2.8 Hz, 1H), 4.85-4.81 (m, 1H), 4.42 (t, J = 6.8 Hz, 1H), 4.14 (d, J = 2 Hz, 1H), 3.75(d, J = 6.4 Hz, 1H), 3.09 - 3.07 (m, 1H), 2.52(m, 1H), 4.12 (t, J = 6.0 Hz, 1H), 2.17-1.39 (m, 8H).
m/z calcd for [C20H22F5N3O4S]+ [M+H]+: 496.0; found: 496.0.

Example 77


n-Butyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0495] 



[0496] Sodium methoxide (2.7 mg, 0.05 mmol) was added to a solution of n-Butyl 2,4,6-triO-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (300 mg, 0.54 mmol) in MeOH (5.0 mL) at room temperature. The mixture was stirred at room temperature for 1h. The mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered and concentrated in vacuo to afford crude product, which was purified by preparative-HPLC to afford the title compound (163.6 mg, 70% yield).
1H NMR (400 MHz, MeOD) δ 8.36 (s, 1H), 7.58 - 7.42 (m, 2H), 5.42 (d, J= 5.4 Hz, 1H), 4.79 (dd, J = 11.3, 2.8 Hz, 1H), 4.66 (dd, J = 11.3, 5.4 Hz, 1H), 4.25 (t, J = 6.1 Hz, 1H), 3.97 (d, J = 2.1 Hz, 1H), 3.59 (d, J = 6.1 Hz, 2H), 2.53 (dtd, J = 20.2, 12.8, 7.5 Hz, 2H), 1.60 - 1.45 (m, 2H), 1.40 - 1.27 (m, 2H), 0.81 (t, J = 7.4 Hz, 3H).
ESI-MS m/z calcd for [C18H22F3N3O4S]+ (M+H)+: 433.1; found: 434.2.

Example 78


3,4-Dichlorophenyl 3-deoxy-3-[4-(3,5-difluoro-4-methoxyphenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0497] 



[0498] To a stirred solution of 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,5-difluoro-4-methoxyphenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (150 mg) in MeOH (5 mL), NaOMe (12.35 mg) was added. The mixture was stirred at rt for 2 hours. The reaction mixture was Neutralized with H+ resin, filtered and concentrated to afford product as a syrup. The crude product was purified by prep-HPLC to afford the title compound (50 mg, 45% yield).
1H NMR (400 MHz, DMSO) δ 8.73 (s, 1H), 7.84 (d, J= 2.1 Hz, 1H), 7.74 - 7.47 (m, 4H), 5.90 (dd, J = 15.5, 4.5 Hz, 2H), 5.51 (d, J = 6.4 Hz, 1H), 4.90 - 4.64 (m, 3H), 4.25 (t, J = 6.1 Hz, 1H), 4.02 (d, J = 4.8 Hz, 1H), 3.96 (s, 3H), 3.60 - 3.50 (m, 1H), 3.48-3.37 (m, 1H).
ESI-MS m/z calcd for [C21H19Cl2F2N3O5S]+ [M+H]+: 533.0; found: 534.2.

Example 79


2-hydroxy-pyridin-4-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0499] 



[0500] 2-hydroxy-pyridin-4-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (100 mg) was dissolved in MeOH (10 mL). NaOMe (0.05 g) was added. The mixture was stirred at rt for 30 min. The reaction mixture was concentracted and the residue was purified by preparative HPLC to give 20 mg (28%) of of the title compound.
1H NMR (400 MHz, DMSO) δ 11.39 (s, 1H), 8.80 (s, 1H), 7.84 (dd, J = 9.0, 6.8 Hz, 2H), 7.27 (d, J = 6.9 Hz, 1H), 6.43 (d, J = 1.7 Hz, 1H), 6.23 (dd, J = 7.0, 1.8 Hz, 1H), 6.07 (d, J = 2.6 Hz, 1H), 5.91 (s, 1H), 5.56 (s, 1H), 4.78 (s, 2H), 4.71 (s, 1H), 4.13 (t, J = 6.4 Hz, 1H), 4.02 (s, 1H), 3.65 - 3.50 (m, 1H), 3.49 - 3.38 (m, 1H).
ESI-MS m/z calcd for [C19H18F3N4O5S]+ (M+H)+: 471.1; found: 471.2.

Example 80


2-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0501] 



[0502] 2-chlorophenetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (100 mg, 0.16 mmol) was dissolved in MeOH (12 mL). NaOMe (8.5mg, 0.16 mmol) was added. The mixture was stirred at room temperature for 16 hours. Then the mixture was concentrated and the residue was purified on reverse phase column using a gradient of acetonitrile/0.01M NH4HCO3 from 0-52% to give 30 mg (37%) of the title compound.
1H NMR (400 MHz, DMSO) δ 8.76 (s, 1H), 7.84 (dd, J= 9.0, 6.8 Hz, 2H), 7.44 (dd, J = 7.7, 1.5 Hz, 2H), 7.34 - 7.16 (m, 2H), 5.60 (d, J= 5.2 Hz, 2H), 5.43 (d, J= 6.1 Hz, 1H), 4.77 - 4.69 (m, 2H), 4.69 - 4.52 (m, 1H), 4.16 (t, J = 6.2 Hz, 1H), 4.05 - 3.94 (m, 1H), 3.67 - 3.39 (m, 2H), 3.04 (t, J= 7.7 Hz, 2H), 2.93 - 2.70 (m, 2H).
ESI-MS m/z calcd for [C22H22ClF3N3O4S]+
(M+H)+: 516.1; found: 516.1.

Example 81


4-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0503] 



[0504] 4-Chlorophenetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (100 mg, 0.16 mmol) was dissolved in MeOH (12 mL). NaOMe (8.5mg, 0.16 mmol) was added. The mixture was stirred at rt for 16 hours. Then the mixture was concentrated and the residue was purified on C18 column to give 30 mg (37.3%) of the title compound as a white solid.
1H NMR (400 MHz, DMSO) δ 8.75 (s, 1H), 7.83 (dd, J = 9.0, 6.8 Hz, 2H), 7.45 - 7.20 (m, 4H), 5.57 (d, J = 5.2 Hz, 2H), 5.43 (s, 1H), 4.87 - 4.72 (m, 2H), 4.72 - 4.60 (m, 1H), 4.14 (t, J = 6.2 Hz, 1H), 3.96 (s, 1H), 3.65 - 3.43 (m, 2H), 2.96 - 2.71 (m, 4H).
ESI-MS m/z calcd for [C22H22ClF3N3O4S]+ (M+H)+: 516.1; found: 516.1

Example 82


2-Chlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0505] 



[0506] 2-Chlorobenzyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (132 mg) was dissolved in MeCN (10 mL). Copper(I) iodide (5 mg) and 3,4,5-trifluorophenylacetylene (0.100 mL) were added and the mixture degassed (N2). After 5 min, DIEA (0.200 mL) was added and the resulting mixture stirred over night. It was then filtered through a small plug of silica and concentrated in vacuo. The residue was suspended in MeOH (40 mL). 1M sodium methoxide in MeOH (1 mL) was added. After stirring at r.t. 2 h, acetic acid (1 mL) was added and the mixture concentrated in vacuo. The residue was dissolved in small volume MeCN/water, filtered and purified by HPLC (C18/H2O:MeCN:0.1% TFA). Freezedrying afforded the title compound as a white powder (123 mg). 1H NMR (400 MHz, Methanol-d4) δ 8.49 (s, 1H), 7.61 (dd, J = 8.6, 6.6 Hz, 2H), 7.52 (dd, J = 6.4, 2.9 Hz, 1H), 7.40 (dd, J = 6.5, 2.8 Hz, 1H), 7.31 -7.20 (m, 2H), 5.50 (d, J = 5.4 Hz, 1H), 4.97 (dd, J = 11.3, 2.8 Hz, 1H), 4.82 (dd, J = 11.3, 5.5 Hz, 1H), 4.46 (t, J = 6.1 Hz, 1H), 4.17 (d, J = 2.8 Hz, 1H), 4.03 - 3.90 (m, 2H), 3.77 (d, J = 6.1 Hz, 2H). ESI-MS m/z calcd for [C21H20ClF3N3O4S]+ (M+H)+: 502.07; found: 502.10.

Example 83


3,4-Dichlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0507] 



[0508] 3,4-Dichlorobenzyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (123 mg) was dissolved in MeCN (10 mL). Copper(I) iodide (10 mg) and 3,4,5-trifluorophenylacetylene (0.100 mL) were added and the mixture degassed (N2). After 5 min, DIEA (0.200 mL) was added and the resulting mixture stirred over night. It was then filtered through a small plug of silica and concentrated in vacuo. The residue was suspended in MeOH (40 mL). 1M sodium methoxide in MeOH (1 mL) was added. After stirring at r.t. 2 h, acetic acid (1 mL) was added and the mixture concentrated in vacuo. The residue was dissolved in a small volume of MeCN/water, filtered and purified by HPLC (C18/H2O:MeCN:0.1% TFA). Freezedrying afforded the title compound as a white powder (84 mg). 1H NMR (400 MHz, Methanol-d4) δ 8.49 (s, 1H), 7.68 - 7.57 (m, 3H), 7.46 (d, J = 8.2 Hz, 1H), 7.36 (dd, J = 8.3, 1.9 Hz, 1H), 5.36 (d, J = 5.5 Hz, 1H), 4.97 (dd, J = 11.3, 2.9 Hz, 1H), 4.80 (dd, J = 11.3, 5.5 Hz, 1H), 4.41 (t, J = 6.1 Hz, 1H), 4.15 (d, J = 2.8 Hz, 1H), 3.91 - 3.83 (m, 1H), 3.83 - 3.71 (m, 3H). ESI-MS m/z calcd for [C21H19Cl2F3N3O4S]+ (M+H)+: 536.03; found: 536.00.

Example 84


3-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0509] 



[0510] 3-Chlorophenetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (120 mg, 0.19 mmol) was dissolved in MeOH (12 mL). NaOMe (10.1 mg, 0.19 mmol) was added. The mixture was stirred at rt for 16 hours. Then the mixture was concentrated and the residue was purified on C18 column using a gradient of aectonitrile/ 0.01M NH4HCO3 from 0-52% to give 33 mg (34.2%) of the title compound as a white solid.
1H NMR (400 MHz, DMSO) δ 8.76 (s, 1H), 7.84 (dd, J = 9.0, 6.8 Hz, 2H), 7.39 - 7.23 (m, 4H), 5.58 (t, J = 5.7 Hz, 2H), 5.42 (d, J = 6.4 Hz, 1H), 4.81 - 4.70 (m, 2H), 4.70 - 4.60 (m, 1H), 4.15 (t, J = 6.1 Hz, 1H), 3.96 (dd, J = 6.1, 2.5 Hz, 1H), 3.64 - 3.45 (m, 2H), 3.01 - 2.71 (m, 4H).
ESI-MS m/z calcd for [C22H22ClF3N3O4S]+ (M+H)+: 516.1; found: 516.1.

Example 85


4-Chlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0511] 



[0512] 4-Chlorobenzyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (98 mg) was dissolved in MeCN (10 mL). Copper(I) iodide (5 mg) and 3,4,5-trifluorophenylacetylene (0.100 mL) were added and the mixture degassed (nitrogen). After 5 min, DIEA (0.200 mL) was added and the resulting mixture stirred over night. It was then filtered through a small plug of silica and concentrated in vacuo. The residue was suspended in MeOH (40 mL). 1M sodium methoxide in MeOH (1 mL) was added. After stirring at r.t. 2 h, acetic acid (1 mL) was added and the mixture concentrated in vacuo. The residue was dissolved in small volume MeCN/water, filtered and purified by HPLC (C18/H2O:MeCN:0.1% TFA). Freezedrying afforded the title compound as a white powder (85 mg). 1H NMR (400 MHz, Methanol-d4) δ 8.48 (s, 1H), 7.62 (dd, J = 8.5, 6.7 Hz, 2H), 7.41 (d, J = 8.1 Hz, 2H), 7.35 - 7.27 (m, 2H), 5.38 (d, J = 5.4 Hz, 1H), 4.97 (dd, J = 11.4, 2.9 Hz, 1H), 4.80 (dd, J = 11.3, 5.6 Hz, 1H), 4.43 (t, J= 6.0 Hz, 1H), 4.15 (d, J= 2.9 Hz, 1H), 3.92 - 3.83 (m, 1H), 3.82 - 3.72 (m, 3H). ESI-MS m/z calcd for [C21H20ClF3N3O4S]+ (M+H)+: 502.07; found: 502.10.

Example 86


Propyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0513] 



[0514] MeONa (1.08 mg, 0.02 mmol) was added to a solution of propyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (100 mg, 0.18 mmol) in MeOH (5.0 mL) at room temperature. The mixture was stirred at room temperature for 1 h. The mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered and concentrated in vacuo to afford crude product, which was purified by Preparative HPLC to afford the title compound (68.7 mg, 91% yield).
1H NMR (400 MHz, MeOD) δ 8.36 (s, 1H), 7.49 (dd, J = 8.8, 6.7 Hz, 2H), 5.42 (d, J = 5.4 Hz, 1H), 4.79 (dd, J = 11.3, 2.9 Hz, 1H), 4.66 (dd, J = 11.3, 5.4 Hz, 1H), 4.25 (t, J = 6.0 Hz, 1H), 3.97 (d, J = 2.3 Hz, 1H), 3.59 (d, J = 6.1 Hz, 2H), 2.50 (dtd, J = 20.3, 12.9, 7.5 Hz, 2H), 0.90 (t, J = 7.3 Hz, 3H).
ESI-MS m/z calcd for [C17H20F3N3O4S]+ (M+H)+: 419.1; found: 420.2.

Example 87


2-Aminopyridin-4-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0515] 



[0516] Sodium methoxide (3.37 mg, 0.06 mmol) was added to a solution of 2-Aminopyridin-4-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (124 mg, 0.21 mmol) in MeOH (5.0 mL) at room temperature. The mixture was stirred at room temperature for 1 h. The mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered and concentrated in vacuo to afford crude product, which was purified by Prep-HPLC to afford the title compound (4.95 mg, 5.06% yield).
1H NMR (400 MHz, MeOD) δ 8.46 (s, 1H), 7.64 (d, J = 5.5 Hz, 1H), 7.56 (dd, J = 8.9, 6.7 Hz, 2H), 6.73 (s, 1H), 6.66 (dd, J = 5.8, 1.6 Hz, 1H), 6.00 (d, J = 4.4 Hz, 1H), 4.91 - 4.82 (m, 2H), 4.29 (t, J = 6.1 Hz, 1H), 4.09 (d, J = 2.4 Hz, 1H), 3.69 - 3.58 (m, 2H).
ESI-MS m/z calcd for [C19H18F3N5O4S]+ (M+H)+: 469.1; found: 470.2.

Example 88


5-dimethylamino-naphatlen-2-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0517] 



[0518] To a solution of 5-dimethylamino-naphatlen-2-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (50 mg, 0.07 mol) in MeOH/H2O (0.6 mL) was added TEA (0.3 ml). The reaction was held at room temperature with stirring on for 2 h. The solvent was evaporated to dryness. The residue was purified by preparative-HPLC to obtain the desired product.
1H NMR (400 MHz, MeOD) δ 8.41 (s, 1H), 8.01 (d, J = 8.9 Hz, 1H), 7.94 (d, J = 1.7 Hz, 1H), 7.58 - 7.43 (m, 3H), 7.33 (d, J = 8.2 Hz, 1H), 7.24 (t, J = 7.8 Hz, 1H), 6.97 (d, J = 6.9 Hz, 1H), 5.74 (d, J = 5.3 Hz, 1H), 4.89 (dd, J = 11.4, 2.8 Hz, 1H), 4.81 - 4.76 (m, 1H), 4.48 (t, J = 6.2 Hz, 1H), 4.07 (d, J = 2.0 Hz, 1H), 3.68 - 3.43 (m, 2H), 2.71 (s, 6H).
m/z calcd for [C26H25F3N4O4S]+ [M+H]+: 547.0; found: 547.0.

Example 89


Ethyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0519] 



[0520] Ethyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (43 mg) was dissolved in MeCN (5 mL) and stirred at r.t. under N2. Copper(I) iodide (9 mg) was added and after five minutes 1,2,3-trifluoro-5-[2-(trimethylsilyl)ethynyl]benzen (0.050 mL) was added. After an additional five minutes DIEA (0.020 mL) was added and the mixture was heated to 45 °C. After 2 h the heat was increased to 80 °C for 70 min and then decreased to 30 °C. After three days the reaction mixture was filtered through a short column of silica, eluting with EtOAc and then concentrated in vacuo. The crude product was dissolved in methanolic NaOMe (0.05M, 20 mL) and stirred at r.t. After 100 min acetic acid (2 mL) was added and the mixture was concentrated in vacuo. The crude product was purified by preparative HPLC (C18/H2O:MeCN:0.1% TFA) and lyophilized to give 42 mg of ethyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside. 1H NMR (400 MHz, Methanol-d4) δ 8.50 (s, 1H), 7.72 - 7.58 (m, 2H), 5.61 (d, J = 5.4 Hz, 1H), 4.94 (dd, J = 11.3, 2.3 Hz, 1H), 4.85 - 4.77 (m, 1H), 4.40 (t, J = 6.0 Hz, 1H), 4.13 (s, 1H), 3.74 (d, J = 6.1 Hz, 2H), 2.80 - 2.59 (m, 2H), 1.34 (t, J = 7.4 Hz, 3H). 13C NMR (101 MHz, Methanol-d4) δ 123.0, 110.9, 110.6, 86.9, 72.5, 69.8, 66.6, 65.6, 62.4, 24.5, 15.2. ESI-MS m/z calcd for [C16H19F3N3O4S]+ (M+H)+: 406.1; found: 406.1.

Example 90


5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide, isomer 1



[0521] 



[0522] NaOMe (2.32 mg, 0.04 mmol) was added in to a mixture of 5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide , isomer 1 (145 mg, 0.21 mmol) in MeOH (10 mL). Then it was stirred at room temperature for 1 h. The mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered, washed with MeOH (20 mL) and concentrated in vacuo to afford crude product, which was purified by trituration in CH2Cl2 and Et2O to afford 1,3-dideoxy-1-(5-bromopyridine-3-yl-thio)-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galacto pyranoside (69.41 mg, 58.86% yield). The chirality of the stereocenter located at the sulfurus atom marked * was not determined.
1H NMR (400 MHz, MeOD) δ 8.93 (d, J = 1.8 Hz, 1H), 8.85 (d, J = 2.1 Hz, 1H), 8.56 (s, 1H), 8.50 (t, J = 1.9 Hz, 1H), 7.79-7.51 (m, 1H), 5.61 (dd, J = 11.1, 2.6 Hz, 1H), 5.18 (dd, J = 11.0, 5.6 Hz, 1H), 5.09 (d, J = 5.6 Hz, 1H), 4.27 (d, J = 2.3 Hz, 1H), 4.21 (t, J= 5.9 Hz, 1H), 3.64 - 3.46 (m, 1H).
ESI-MS m/z calcd for [C19H16BrF3N4O5S]+ [M+H] +: 548.0; found: 549.0

Example 91


5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide, isomer 2



[0523] 



[0524] NaOMe (0.64 mg, 0.01 mmol) was added in to a mixture of 5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide, isomer 2 (40 mg, 0.06 mmol) in MeOH (5 mL). Then it was stirred at room temperature for 1 h. The mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered, washed with MeOH (20 mL) and concentrated in vacuo to afford crude product, which was purified by trituration in CH2Cl2 and Et2O to afford the title compound (3.32 mg, 10.21 % yield). The chirality of the stereocenter located at the sulfurus atom marked * was not determined.
1H NMR(400 MHz, MeOD) δ 8.84 (dd, J = 13.6, 1.9 Hz, 1H), 8.62 (s, 1H), 8.49 (t, J = 2.0 Hz, 1H), 7.69 (dd, J = 8.8, 6.6 Hz, 1H), 5.77 (dd, J = 11.2, 2.7 Hz, 1H), 5.24 (dd, J = 11.3, 6.6 Hz, 1H), 5.02 (t, J = 6.0 Hz, 1H), 4.95 (d, J = 6.5 Hz, 1H), 4.26 (s, 1H), 3.56 (s, 1H), 3.56 - 3.50 (m, 1H).
ESI-MS m/z calcd for [C19H16BrF3N4O5S]+ [M+H] +: 548.0; found: 549.0.

Example 92


5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone



[0525] 



[0526] 5-Bromo-3-pyridyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (9 mg) was dissolved/suspended in CH2Cl2 (3 mL) at r.t. and m-chloroperbenzoic acid (4.6 mg in 0.460 mL CH2Cl2) was added. After 20 h more m-chloroperbenzoic acid (8.4 mg in 0.840 mL CH2Cl2) was added. After 4 h the temperature was increased to 30 °C for an additional 20 h. MeCN (1 mL) was added followed by H2O2 (27%, 0.5 mL) and the mixture was stirred for 6.5 h. The mixture was filtered through a short silica column, eluting with 15% MeOH in EtOAc, concentrated in vacuo. The crude product was purified by preparative HPLC (C18/H2O:MeCN:0.1% TFA) and lyophilized to give 3 mg of the title compound.
1H NMR (400 MHz, Methanol-d4) δ 9.07 (s, 1H), 8.97 (s, 1H), 8.56 (d, J = 9.3 Hz, 2H), 7.70-7.61 (m, 2H), 5.73 (d, J = 11.5 Hz, 1H), 5.43 (d, J = 6.4 Hz, 1H), 5.15 (dd, J = 11.4, 6.5 Hz, 1H), 4.55 (s, 1H), 4.28 (s, 1H), 3.62 (d, J = 6.2 Hz, 2H). 13C NMR (101 MHz, Methanol-d4) δ 156.3, 148.9, 140.9, 122.8, 121.9, 110.9, 93.8, 78.1, 69.2, 66.2, 64.1, 62.3. ESI-MS m/z calcd for [C19H17BrF3N4O6S]+ (M+H)+: 565.0; found: 565.0.

Example 93


5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide, isomer 1



[0527] 



[0528] To a solution of 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide , isomer 1 (18 mg, 0.03 mmol) in MeOH/Et3N/H2O (5/3/1)(0.5 mL) was stirred at room temperature for 4 h. The mixture was evaporated to dryness, and the residue was triturated with ether and filtered to afford the title compound as a white solid (5mg, 34.72%). The chirality of the stereocenter located at the sulfurus atom marked * was not determined.
1H NMR (400 MHz, MeOD) δ 8.79 (d, J = 1.7 Hz, 1H), 8.76 (d, J = 2.3 Hz, 1H), 8.62 (s, 1H), 8.36 (t, J = 2.0 Hz, 1H), 7.69 (dd, J = 8.8, 6.6 Hz, 2H), 5.77 (dd, J = 11.3, 2.7 Hz, 1H), 5.24 (dd, J = 11.3, 6.6 Hz, 1H), 5.02 (t, J = 5.9 Hz, 1H), 4.96 (d, J = 6.6 Hz, 1H), 4.26 (d, 1H), 3.60 - 3.47 (m, 2H).
m/z calcd for [C19H16ClF3N4O5S]+ [M+H]+: 505.0; found: 505.0.

Example 94


5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide, isomer 2



[0529] 



[0530] To a solution of 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide, isomer 2 (100 mg, 0.16 mmol) in MeOH/Et3N/H2O (5/3/1)(2 mL) was stirred at temperature for 4 h. The mixture was evaporated to dryness, and the residue was triturated with ether and filtered to afford the title compound as a white solid (47 mg, 59%). The chirality of the stereocenter located at the sulfurus atom marked * was not determined.
1H NMR (400 MHz, MeOD) δ 8.73 (d, J = 1.8 Hz, 1H), 8.58 (d, J = 2.2 Hz, 1H), 8.39 (s, 1H), 8.20 (t, J = 20 Hz, 1H), 7.50 (dd, J = 8.8, 6.7 Hz, 2H), 5.44 (dd, J = 11.1, 2.8 Hz, 1H), 5.01 (dd, J = 11.1, 5.6 Hz, 1H), 4.92 (d, J = 5.6 Hz, 1H), 4.10 (d, J = 2.0 Hz, 1H), 4.03 (t, J = 5.8 Hz, 1H), 3.39 (qd, J = 11.6, 5.9 Hz, 2H).
m/z calcd for [C19H16ClF3N4O5S]+ [M+H]+: 505.0; found: 505.0.

Example 95


5-Dimethylamino-naphtalen-2-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-l-yl]-α-D-galactopyranosyl sulfone



[0531] 



[0532] To a solution of 5-Dimethylamino-naphtalen-2-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (20 mg, 0.03 mmol) was added TEA (0.3 ml) in MeOH/H2O (0.5 ml/0.1 ml). The reaction was stirred at room temperature for 1 h. The mixture was purified by pre-HPLC to obtain the title compound (6 mg).
1H NMR (400 MHz, MeOD) δ 8.58 (s, 2H), 8.45 (d, J = 9.0 Hz, 1H), 7.99 (dd, J = 9.0, 1.8 Hz, 1H), 7.77 (d, J = 8.2 Hz, 1H), 7.73 - 7.63 (m, 2H), 7.59 (t, J = 7.9 Hz, 1H), 7.36 (d, J = 7.3 Hz, 1H), 5.87 (dd, J = 11.5, 2.8 Hz, 1H), 5.36 (d, J = 6.3 Hz, 1H), 5.20 - 5.11 (m, 1H), 4.65 (t, J = 6.1 Hz, 1H), 4.33 (d, J = 1.8 Hz, 1H), 3.62 - 3.56 (m, 1H), 3.53 - 3.46 (m, 1H), 2.92 (s, 6H).
m/z calcd for [C26H25F3N4O6S]+ [M+H]+: 578.0; found: 578.0

Example 96 (compound not according to the invention) 3,4-dichlorophenyl-3-deoxy-3-(3,4,5-trifluorobenzamido)-1-thio-α-D-galactopyranoside



[0533] 



[0534] To a stirred solution of 3,4-dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-(3,4,5-trifluorobenzamido)-1-thio-α-D-galactopyranoside (50 mg, 0.08 mmol) in MeOH (2 mL) and DCM (0.5 mL) at 0 °C was added sodium methoxide (13 mg, 0.37 mmol). The mixture was stirred at rt for 2 hours. The was adjusted with 2M HCl to pH=7. The mixture was concentrated and the residue was purified on reversed phase column. To give the title compound(18 mg, 45% yield).
1H NMR (400 MHz, DMSO) δ 8.52 - 8.39 (m, 1H), 7.96 - 7.84 (m, 2H), 7.79 (d, J = 2.0 Hz, 1H), 7.57 (d, J= 8.4 Hz, 1H), 7.48 (dd, J = 8.4, 2.1 Hz, 1H), 5.81 (d, J= 5.3 Hz, 1H), 5.40 (d, J = 4.4 Hz, 1H), 5.14 (d, J = 5.4 Hz, 1H), 4.64 (t, J = 5.7 Hz, 1H), 4.38 - 4.26 (m, 1H), 4.14 - 3.95 (m, 2H), 3.92 - 3.87 (m, 1H), 3.60 - 3.47 (m, 1H), 3.36 (s, 1H).ESI-MS m/z calcd for [C19H17Cl2F3NO5S]+ (M+H)+: 498.0; found: 498.1

Example 97


3,4-Dichlorophenyl 3-deoxy-3-[4-(ethoxycarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0535] 



[0536] 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3 -[4-ethoxycarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (117 mg, 0.20 mmol) was dissolved in EtOH (10 mL). TEA (200 mg) and water (1 ml) were added. The mixture was stirred at 90°C for 48 hours. The mixture was concentrated in vacuo and the residue was purified by prep-HPLC to give 22 mg of the title compound.
1H NMR (400 MHz, DMSO) δ 8.66 (s, 1H), 7.83 (d, J = 2.0 Hz, 1H), 7.60 (d, J = 8.4 Hz, 1H), 7.52 (dd, J = 8.5, 2.0 Hz, 1H), 5.97 - 5.83 (m, 2H), 5.45 (d, J = 6.8 Hz, 1H), 4.90 - 4.78 (m, 2H), 4.74 (t, J= 5.6 Hz, 1H), 4.32 (q, J= 7.1 Hz, 2H), 4.24 (t, J= 6.2 Hz, 1H), 4.01 (d, J = 6.2 Hz, 1H), 3.60 - 3.49 (m, 1H), 3.44 - 3.36 (m, 1H), 1.31 (t, J = 7.1 Hz, 3H).
ESI-MS m/z calcd for [C17H20Cl2N3O6S]+ (M+H)+: 464.0; found: 464.0.

Example 98


3,4-Dichlorophenyl 3-deoxy-3-[4-(ethylaminocarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0537] 



[0538] 3,4-Dichlorophenyl 3 -deoxy-3 -[4-carboxy)-1H-1,2,3 -triazol-1-yl]-1-thio-α-D-galactopyranoside (80 mg, 0.18 mmol), 2,2,2-trifluoroethanamine (36 mg, 0.37 mmol) was dissolved in DMF (2 mL). HATU (140 mg, 0.37 mmol ), N-ethyl-N-isopropyl-propan-2-amine (120 mg, 0.92 mmol) were added. The reaction mixture was stirred at rt for 2hours followed by purification by preparative-HPLC to give 40 mg of the title compound.
1H NMR (400 MHz, DMSO) δ 9.18 (t, 1H), 8.58 (s, 1H), 7.83 (d, J = 2.0 Hz, 1H), 7.66 - 7.45 (m, 2H), 5.89 (d, J = 5.0 Hz, 2H), 5.56 - 5.38 (m, 1H), 4.93 - 4.68 (m, 2H), 4.24 (t, J= 6.2 Hz, 1H), 4.15 - 3.90 (m, 3H), 3.58 - 3.44 (m, 2H).
ESI-MS m/z calcd for [C17H18Cl2F3N4O5S]+ (M+H)+: 517.0; found: 517.0.

Example 99 (compound not according to the invention)


3,4-Dichlorophenyl 3-O-[(5,6-Difluoro-2-oxo-3-chromenyl)methyl] -1-thio-α-D-galactopyranoside



[0539] 



[0540] To a solution of 3,4-dichlorophenyl-2,4,6-tri-O-acaetyl-3-O-propargyl-1-thio-α-D-galactopyranoside (300 mg, 0.59 mmol) in tetrahydrofuran (15 mL) was added 2,3-difluoro-6-hydroxybenzaldehyde (93.85 mg, 0.59 mmol), N-diazo-4-methyl-benzenesulfonamide (117,07 mg, 0,5mmol), copperiodide (11.31 mg, 0.06 mmol). The reaction mixture was purged three times with N2. The reaction mixture was stirred at room temperature for 1h. N,N-diethylethanamine (120.14 mg, 1,19 mmol) was added via addition funnel to the mixture. Then the mixture was stirred at room temperature for 20h. Solvents were evaporated in vacuo and the residue was dissolved in DCM (150 mL) and washed successively with aqueous NH4Cl (2 X 30mL) and brine (30X 2 mL). The organic layer was separated, dried over Na2SO4 and evaporated in vacuo. The residue was purified by flash chromatography (PE:EtOAc=2:3) to give the corresponding tosyl sulfoninimid intemediate 290 mg ( 59.97 %) as yellow oil. ESI-MS m/z calcd for [C35H31Cl2F2NO11S2]+ [M+H]+: 814.0; found: 814.0.

[0541] The intermediate tosyl sulfonamide intermediate (55 mg, 0.07 mmol) was dissolved in NaOCH3/methanol (5 mL,0.05 M). Then the mixture was stirred at room temperature for 20 h. After completion, DOWEX50wx8-200 Ion exchange resin was added (PH=7) and the mixture was filtered. The filtrate was concentrated in vacuo followed by purification by preparative HPLC to give the title compound 5 mg (14,2%) as a white solid.
1HNMR (500 MHz, DMSO-d6) δ 8.34 (s, 1H), 7.81 -7.67 (m, 2H), 7.55 (d, J = 8.4 Hz, 1H), 7.46 (dd, J = 8.5, 2.1 Hz, 1H), 7.36 (d, J = 6.9 Hz, 1H), 5.73 (t, J = 5.3 Hz, 2H), 4.92 (d, J = 4.9 Hz, 1H), 4.74 - 4.47 (m, 3H), 4.32 - 4.19 (m, 1H), 4.11 (m, 1H), 4.01 (t, J = 6.1 Hz, 1H), 3.58 (dt, J = 11.6, 5.9 Hz, 1H), 3.49 (dd, J = 10.1, 2.9 Hz, 1H), 3.45 - 3.38 (m, 1H). ESI-MS m/z calcd for [C22H18Cl2F2O7S]+ [M+H]+: 534.0; found: 534.0.

Example 100


5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0542] 



[0543] Sodium Methoxide (1.09 mg, 0.02 mmol) was added to a solution of 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (120 mg, 0.20 mmol) in MeOH (5.0 mL) at room temperature. The mixture was stirred at room temperature for 1h. The mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered, washed with MeOH (20 mL) and concentrated in vacuo to afford crude product, which was purified by preparative-HPLC to afford the title compound (82.5 mg, 87% yield).
1H NMR (400 MHz, MeOD) δ 8.66 (d, J= 1.8 Hz, 1H), 8.51 (s, 1H), 8.48 (t, J= 4.6 Hz, 1H), 8.21 (t, J = 2.1 Hz, 1H), 7.80 (ddd, J = 11.7, 7.7, 2.1 Hz, 1H), 7.72 - 7.64 (m, 1H), 7.37 (dt, J = 10.4, 8.5 Hz, 1H), 5.94 (d, J = 5.1 Hz, 1H), 5.04 (dd, J = 11.4, 2.7 Hz, 1H), 4.99 - 4.92 (m, 1H), 4.51 (t, J = 6.1 Hz, 1H), 4.22 (d, J = 1.7 Hz, 1H), 3.78 - 3.66 (m, 2H).
ESI-MS m/z calcd for [C19H17ClF2N4O4S]+ [M+H] +: 470.1; found: 471.0.

Example 101


5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0544] 



[0545] Sodium methoxide (0.84 mg, 0.02 mmol) was added to a solution of Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (100 mg, 0.16 mmol) in MeOH (5.0 mL) at room temperature. The mixture was stirred at room temperature for 1 h. The mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered, washed with MeOH (20 mL) and concentrated in vacuo to afford crude product, which was purified by Prep-HPLC to afford 1,3-dideoxy-1-(5-bromopyridine-3-yl-thio)-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galacto pyranoside (10.96 mg, 13.64% yield).
1H NMR (400 MHz, MeOD) δ 8.70 (d, J = 1.9 Hz, 1H), 8.58 (d, J= 2.1 Hz, 1H), 8.51 (s, 1H), 8.35 (t, J = 2.0 Hz, 1H), 7.80 (ddd, J = 11.7, 7.7, 2.1 Hz, 1H), 7.73 - 7.63 (m, 1H), 7.37 (dt, J = 10.4, 8.4 Hz, 1H), 5.93 (d, J = 5.1 Hz, 1H), 5.03 (dd, J = 11.4, 2.7 Hz, 1H), 4.96 (dd, J = 11.4, 5.2 Hz, 1H), 4.51 (t, J = 6.0 Hz, 1H), 4.22 (d, J = 1.7 Hz, 1H), 3.77-3.63 (m, 2H).
ESI-MS m/z calcd for [C19H17BrF2N4O4S]+ [M+H]+: 514.0; found: 515.1.

Example 102


3,4-Dichlorophenyl 3-deoxy-3-[4-(propyl-carbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0546] 



[0547] To a stirred solution of 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(propyl-carbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (300 mg, 0.51mmol) in MeOH (5 mL), Sodium methoxide (8 mg, 0.15mmol) was added at 0 °C. The mixture was stirred at rt for 0.5 hours. LCMS showed that the target product was formed and no SM left. The reaction was purified by prep-HPLC to give the title compound 25 mg.
1H NMR (400 MHz, DMSO) δ 8.66 (s, 1H), 7.83 (d, J = 2.0 Hz, 1H), 7.60 (d, J = 8.4 Hz, 1H), 7.52 (dd, J = 8.5, 2.1 Hz, 1H), 5.89 (t, J = 4.2 Hz, 2H), 5.44 (d, J = 6.8 Hz, 1H), 4.89 - 4.76 (m, 2H), 4.73 (t, J = 5.6 Hz, 1H), 4.24 (t, J = 6.4 Hz, 1H), 4.00 (d, J = 6.1 Hz, 1H), 3.58 - 3.47 (m, 1H), 3.46 - 3.36 (m, 1H), 2.99 (t, J = 7.2 Hz, 2H), 1.86 - 1.52 (m, 2H), 0.93 (t, J = 7.4 Hz, 3H).
ESI-MS m/z calcd for [C18H22Cl2N3O5S]+ (M+H)+: 462.1; found: 462.0.

Example 103


5-Chloro-6-trifluoromethyl-pyridine-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0548] 



[0549] To a solution of 5-chloro-6-trifluoromethyl-pyridine-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (110 mg, 0.21 mmol) in MeCN (15 mL) was added 3,4,5-trifluorophenylacetylene (71.49 mg, 0.32 mmol), iodocopper (11.93 mg, 0.06 mmol), CsF (47.57 mg, 0.31 mmol) and N,N-diethylethanamine (134.92 mg, 1.04 mmol). The reaction vessel was purged 3 times with nitrogen. Then the mixture was stirred at room temperature for 2 h. The mixture was filtered and washed with EtOAc (50 mL), The filtrated was concentrated in vacuo to afford crude intermediate, which was used for next step directly without further purification. MeONa (1.13 mg, 0.02 mmol) was added to a solution of crude 1,3-dideoxy-2,4,6-tri-O-acetyl-1-(5-chloro-6-(trifluoromethyl)pyridin-3-ylthio)-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside in MeOH (5 mL) at room temperature. The mixture was stirred at room temperature for 1 h. The mixture was acidified with dowex 50 wx8 hydrogen form to PH= 5∼6. The solution was filtered, washed with MeOH (20 mL) and concentrated in vacuo to afford crude product, which was purified by Prep-HPLC, Prep-TLC and chromatography to afford the title compound (15 mg, 13% yield)
1H NMR (400 MHz, MeOD) δ 8.73 (d, J = 1.8 Hz, 1H), 8.58 (s, 1H), 8.36 (d, J = 1.3 Hz, 1H), 7.67 (dt, J = 11.3, 5.6 Hz, 2H), 6.16 (d, J = 5.2 Hz, 1H), 5.07 (dd, J = 11.4, 2.7 Hz, 1H), 4.99 (dd, J = 11.4,5.2 Hz, 1H), 4.42 (t, J = 6.1 Hz, 1H), 4.21 (d, J = 1.7 Hz, 1H), 3.72 (d, J = 6.0 Hz, 2H).
ESI-MS m/z calcd for [C20H15ClF6N4O4S]+ (M+H)+: 556.0; found: 557.2.

Example 104


5-Chloro-2-trifluoromethyl-pyridine-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0550] 



[0551] Following purification by Prep-HPLC, Prep-TLC and chromatography in Example 103, 2.69 mg(2.31% yield) of the title compound was isolated.
1H NMR (400 MHz, MeOD) δ 8.46 (s, 1H), 8.42 (d, J = 2.0 Hz, 1H), 8.40 (s, 1H), 7.56 (dd, J = 8.9, 6.6 Hz, 2H), 5.99 (d, J = 4.7 Hz, 1H), 4.95 - 4.86 (m, 2H), 4.30 (t, J = 6.3 Hz, 1H), 4.11 (s, 1H), 3.60 (dd, J = 6.0, 2.4 Hz, 2H).
ESI-MS m/z calcd for [C20H15ClF6N4O4S]+ (M+H)+: 556.0; found: 557.2.

Example 105


5-Chloro-6-cyano-pyridine-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0552] 



[0553] To a solution of 5-Chloro-6-cyano-pyridine-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (60 mg) in methanol/TEA/H2O (0.5/0.3/0.1 mL) was stirred at room temperature for 4 h. The mixture was evaporated to dryness and the crude material was purified by preparative-HPLC (on C-18 column using a gradient of CH3CN/10mM NH4HCO3 from 0-40%) to obtained the title compound (20 mg, 41.8%). m/z calcd for [C20H16ClF2N5O4S]+ [M+H]+: 496.0; found: 496.0.
1H NMR (400 MHz, MeOD) δ 8.74 (d, J = 1.8 Hz, 1H), 8.52 (s, 1H), 8.36 (d, J = 1.8 Hz, 1H), 7.84 - 7.76 (m, 1H), 7.69 (d, J = 6.9 Hz, 1H), 7.37 (dd, J = 18.9, 8.5 Hz, 1H), 6.23 (d, J = 5.0 Hz, 1H), 5.06 (dd, J = 11.2, 2.6 Hz, 1H), 5.01 (dd, J = 11.4, 5.0 Hz, 1H), 4.37 (t, J = 6.0 Hz, 1H), 4.20 (d, J = 1.2 Hz, 1H), 3.72 (d, J = 6.0 Hz, 2H).

Example 106


3-Chloro-4-cyanophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0554] 

5-Chloro-6-cyano-pyridine-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (70 mg) in methanol/TEA/H2O (2.5/1.5/0.5 mL) was stirred at room temperature for 4 h. The mixture was evaporated to dryness and the crude was purified by prep-HPLC (on C-18 column using a gradient of CH3CN/10mM NH4HCO3 from 0-43%) to obtained the title compound (50 mg, 34.86%).
m/z calcd for [C21H17ClF2N4O4S]+ [M+H]+: 495.0; found: 495.0.
1H NMR (400 MHz, MeOD) δ 8.51 (s, 1H), 7.88 (d, J = 1.5 Hz, 1H), 7.80 (ddd, J = 11.5, 7.6, 2.0 Hz, 1H), 7.70 (dt, J = 8.2, 4.9 Hz, 3H), 7.37 (dt, J = 10.3, 8.5 Hz, 1H), 6.13 (d, J = 4.6 Hz, 1H), 5.03 (dd, J = 11.5, 2.4 Hz, 1H), 4.99 (dd, J = 11.5, 4.6 Hz, 1H), 4.40 (t, J = 6.1 Hz, 1H), 4.22 (d, 1H), 3.79 - 3.67 (m, 2H).

Synthesis of intermediates i57-i106


i57) 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0555] 



[0556] 3,4-dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (300 mg, 0.61 mmol), ethynylbenzene (186.71 mg, 1.83 mmol), copper(I)Iodide (34.81 mg, 0.18 mmol) and triethylamine (308.3 mg, 3.05 mmol) was dissolved in DMF (5 mL). The mixture was stirred at 100°C for 1 hour. EtOAc (200 ml) was added. The reaction mixture was filtered, washed with water (100 mL), dried over sodium sulphate, filtered and the solvents were removed in vacuo. The residue was purified on silica gel column. 250 mg (69%) of 3,4-dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(phenyl)-1-thio-α-D-galactopyranoside was obtained. ESI-MS m/z calcd for [C26H26Cl2N3O7S]+ (M+H)+: 594.1; found: 594.1.

i58) 3,5-Dichloro-4-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0557] 



[0558] To a stirred solution of 3,5-dichloro-4-fluoro-benzenethiol (500 mg, 2.54 mmol) in dry DMF (4 mL) was added NaH (61 mg, 2.54 mmol) at 0°C. The mixture was stirred at room temperature for 30 min. Then 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (591 mg, 1.69 mmol) was added. The mixture was stirred at 50°C overnight. The solvents were removed in vacuo and the resulting crude was diluted with CH2Cl2 and washed with water and brine. The organic layer was dried over Na2SO4, filtered and evaporated. The crude product was purified by chromatography column (PE:EtOAc=3:1) to afford the title compound (120 mg).

i59) 3,4-Dichloro-6-fluorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


4,5-Dichloro-2-fluoroaniline



[0559] 



[0560] 1,2-dichloro-3-fluoro-5-nitro-benzene (5 g, 23.8 mmol), NH4Cl (14 g, 261.9 mmol) was dissolved in EtOH/H2O (160 mL, 15/1). The solution heated to 85°C followed by addition of Fe in portions. After additon the reaction mixture was heated(85°C) for 1h. The reaction mixture was extracted with EtOAc (10 mL x 3). The combined organic layers were washed with brine (15 mL), dried over Na2SO4, and concentrated in vacuum to give crude product. Purification by flash chromatography gave 3,4-dichloro-5-fluoro-aniline (4 g).

S-4,5-dichloro-2-fluorophenyl O-ethyl carbonodithioate



[0561] 



[0562] 4,5-dichloro-2-fluoro-aniline (2.0 g, 11.1 mmol) was dissolved in con HCl (20 mL) at 0°C, Then NaNO2 (770 mg, 11.1mmol) in water (2 mL) was added slowly. The solution was stirred at 0°C until the solution was clear. Potassium ethyl xanthogenate (2.67 g, 16.7 mmol) in water (15 mL) was added to mixture. The solution was stirred at 50°C for 2 hs. The resulting solution was extracted with EtOAc (10 mL x 3). The combined organic layers were washed with brine (15 mL), dried over Na2SO4, and concentrated in vacuo to give crude product, which was purified by flash chromatography to afford S-4,5-dichloro-2-fluorophenyl O-ethyl carbonodithioate (3.0 g)

4,5-dichloro-2-fluorobenzenethiol



[0563] 



[0564] KOH (1.77 g, 32 mmol) was added to a mixture of O-ethyl (4,5-dichloro-2-fluorophenyl)sulfanylmethanethioate (3 g, 10.5 mmol) in EtOH (20 mL). The solution was stirred at 85°C for 2h. The mixture was acified with conc. HCl to pH = 4∼5. The resulting solution was extracted with EtOAc (50 mL x 3). The combined organic layers were washed with brine (50 mL), dried over Na2SO4, and concentrated in vacuum to give crude product, which was purification by flash chromatography to afford 3,5-dichloro-4-fluoro-benzenethio (2 g).

3,4-Dichloro-6-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0565] 



[0566] To a stirred solution of 4, 5-dichloro-2-fluoro-benzenethiol (2.0 g, 10.15 mmol) in dry DMF (4 mL) was added NaH (233.33 mg, 10.15 mmol) at 0°C. The mixture was stirred at rt for 30 min. Then 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (360 mg, 2.66 mmol) was added to the mixture. The mixture was stirred at 50°C overnight. The resulting solution was extracted with CH2Cl2 (10 mL x 3). The combined organic layers were washed with brine (15 mL), dried over Na2SO4, and concentrated in vacuum to give crude product, which was purification by flash chromatgraphy (PE: EtOAc=3:1) to afford the title compound(300 mg, white solid).

3,4-Dichloro-6-fluorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0567] 



[0568] 3,4-Dichloro-6-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (360 mg, 0.71 mmol), trimethyl-[2-(3,4,5-trifluorophenyl)ethynyl]silane (320 mg, 1.41 mmol), Copper(I)Iodide (30 mg, 0.16 mmo) and triethylamine (220 mg, 2.1 mmol) were added to a flask. DMF (3 mL) was added. The mixture was stirred at 50°C for 1 h under a N2 atmosphere. LCMS showed no SM. The resulting solution was extracted with EtOAc (10 mL x 3). The combined organic layers were washed with brine (15 mL), dried over Na2SO4, and concentrated in vacuum to give crude product, which was purification by flash chromatography to give the title compound (160 mg).

i60) 3-Bromo-4-fluorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


O-3-bromo-4-fluorophenyl dimethylcarbamothioate



[0569] 



[0570] To a solution of 3-bromo-4-fluoro-phenol (3000 mg, 15.79 mmol) in DMF (20 mL) were added NaH (180.55 mg, 17.36 mmol). After 10 mins, N,N-dimethylcarbamothioyl chloride (694.73 mg, 7.85 mmol) was added. The reaction mixture was held at room temperature with stirring on for 20h. Water (80 mL) and DCM (80 mL) were added. The organic layer was washed with brine, dried (Na2SO4) and evaporated to dryness. The crude products were purified by flash column chromatography to obtain O-3-bromo-4-fluorophenyl dimethylcarbamothioate (3100 mg, 70.87%) as a white solid.

S-3-bromo-4-fluorophenyl dimethylcarbamothioate



[0571] 



[0572] To a solution of 0-3-bromo-4-fluorophenyl dimethylcarbamothioate (3100 mg, 11.19 mmol) in phenoxybenzene (20 mL) was held at 280 °C with stirring on for 2 h. The reaction mixture was cooled to room temperature. The crude product was purified by flash chromatography to obtain S-3-bromo-4-fluorophenyl dimethylcarbamothioate (2800 mg, 90.32%) as a yellow solid.

2.3 3-bromo-4-fluorobenzenethiol



[0573] 



[0574] To a solution of S-3-bromo-4-fluorophenyl dimethylcarbamothioate (1200 mg, 4.33 mmol) in methanol (15 mL) was added 10% NaOH aq. (8 ml). The reaction was held at 50 °C with stirring on for 4 h. TLC analysis indicated the total consumption of the SM. The reaction mixture was cooled to room temperature and adjusted pH to 3, then DCM (15 ml) was added. The organic layer was evaporated to dryness and used in the next step.

3-Bromo-4-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0575] 



[0576] To a solution of 3-bromo-4-fluorobenzenethiol (891.98 mg, 4.33 mmol) in DMF (10 mL) was added NaH (138.56 mg, 3.46 mmol) at 0 °C under a N2 atmosphere. After 10 min, 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (755.59 mg, 2.17 mmol) was added. The reaction mixture was stirred at 50 °C for 4 h. The mixture was diluted with DCM (30 mL) and washed with 10% citric acid (20 mL). The organic layer was evaporated to dryness. The crude product was purified by flash column chromatography to obtain 3-Bromo-4-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (300 mg, 26.64%) as a white solid.

3-Bromo-4-fluorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0577] 



[0578] To a solution of 3-Bromo-4-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (300 mg, 0.57 mmol) in DMF (6 mL) was added TEA (194.47 mg, 1.92 mmol), Copper(I)Iodide (21.96 mg, 0.12 mmol), 3,4,5-trifluorophenylacetylene (175.49 mg, 0.77 mmol). The reaction was stirred at 100 °C for 2 h. TLC analysis indicated the total consumption of the SM. Water (10 mL) and DCM (20 mL) were added. The aqueous phase was extrated with DCM (20 mL2), and the combined organic phase was washed with brine, dried (Na2SO4) and evaporated to dryness. The crude products were purified by flash column chromatography to give the target compound (250 mg, 64.97%) as a brown solid.

i61) 3-Chloro-4-(trifluoromethyl)phenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside


3-Chloro-4-(trifluoromethyl)-thiophenol



[0579] 



[0580] Sodium sulfide (206 mg) was dissolved in DMF (2 mL) and 2-chloro-4-fluoro-1-(trifluoromethyl)benzene (0.250 mL) was added. After 24 h of stirring, Water was added and the mixture was extracted twice with petroleum ether/EtOAc 1:1, dried Na2SO4 and concentrated to give 382 mg 3-chloro-4-(trifluoromethyl)-thiophenol. The crude product was used in the next step without further purification.

3-Chloro-4-(trifluoromethyl)phenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0581] 



[0582] 3-Chloro-4-(trifluoromethyl)thiophenol (382 mg) was dissolved in DMF (3 mL) and NaH (78 mg, ca 60% in mineral oil) was added and the mixture was stirred at r.t. After 1 h the mixture was added to 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranosid (545 mg) dissolved in DMF (3.5 mL) and the resulting mixture was heated to 55 °C. After 95 min the reaction was removed from heat and allow to reach r.t. slowly over night. The mixture was diluted with EtOAc and washed four times with brine. The aqueous phase was extracted twice with EtOAc and the combined organic phase was concentrated in vacuo. The residue was purified by flash chromatography (SiO2, 0-75% EtOAc in petroleum ether) to give 32 mg 3-chloro-4-(trifluoromethyl)phenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside. ESI-MS m/z calcd for [C19H19ClF3N3O7SNa]+ (M+Na)+: 548.0; found: 548.0.

i62) 3,4,5-Trichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside


3,4,5-Trichlorothiophenol



[0583] 



[0584] Potassium ethyl xanthate (285 mg) was dissolved in water (3 mL) and heated to 70 °C and 3,4,5-trichlorobenzenediazonium tetrafluoroborate (251 mg) was added in portions. After 100 min the mixture was diluted with a small amount of water and extracted three times with Et2O, dried through a phase separator and concentrated in vacuo. The residue was dissolved in methanolic NaOH (0.05 M, 9 mL) and heated to reflux. After 18 h the mixture was allowed to reach r.t. and concentrated in vacuo. The residue was suspended in water (5 mL) and HCl (conc., aq., 5 mL) was added followed by Zn powder (415 mg) and the mixture was heated to reflux for 2 h. Extracted twice with Et2O, dried Na2SO4 and concentrated to give 158 mg of 3,4,5-trichlorothiophenol. 1H NMR (500 MHz, DMSO-d6) δ 7.63 (s, 2H), 6.15 (bs, 1H).

3,4,5-Trichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0585] 



[0586] 3,4,5-Trichlorothiophenol (158 mg) was dissolved in DMF (2 mL) and NaH (88 mg, ca 60% in mineral oil) was added and the mixture was stirred at r.t. After 80 min the mixture was added to 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranosid (130 mg) dissolved in DMF (1.5 mL) and the resulting mixture was heated to 55 °C. After 100 min the reaction was removed from heat and allow to reach r.t. slowly over 3.75 h. The mixture was diluted with EtOAc and washed four times with brine. The aqueous phase was extracted once with EtOAc and the combined organic phase was concentrated in vacuo. The residue was purified by flash chromatography (SiO2, 0-60% EtOAc in petroleum ether) to give 64 mg 3,4,5-trichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside. 1H NMR (400 MHz, Chloroform-d) δ 7.49 (s, 2H), 6.01 (d, J = 5.5 Hz, 1H), 5.48 (d, J = 3.0 Hz, 1H), 5.28 (dd, J = 11.0, 5.5 Hz, 1H), 4.58 (dd, J = 7.6, 4.8 Hz, 1H), 4.14 (dd, J = 11.7, 4.6 Hz, 1H), 4.03 (dd, J = 11.7, 7.9 Hz, 1H), 3.92 (dd, J = 10.9, 3.2 Hz, 1H), 2.19 (s, 3H), 2.17 (s, 3H), 2.01 (s, 3H). ESI-MS m/z calcd for [C18H18Cl3N3O7SNa]+ (M+Na)+: 548.0; found: 548.0.

i63) 5-Chloro-2-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside


5-Chloro-2-fluorothiophenol



[0587] 



[0588] 5-Chloro-2-fluorobenzenesulfonyl chloride (233 mg) was dissolved in CH2Cl2 (20 mL). Triphenylphosphine (841 mg) was added and the mixture was stirred at r.t. After 18 h the mixture was extracted with NaOH (2M). The organic phase was concentrated in vacuo, redissolved in Et2O and extracted once more with NaOH (2M). The combined aqueous phases was acidified to pH 1 with HCl (conc., aq.) and extracted three times with CH2Cl2. The combined organic phase was dried through a phase separator and concentrated to give 141 mg of 5-chloro-2-fluorothiophenol. 1H NMR (400 MHz, DMSO-d6) δ 7.62 - 7.55 (m, 1H), 7.32 - 7.19 (m, 2H), 5.86 (s, 1H).

5-Chloro-2-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0589] 



[0590] 5-Chloro-2-fluorothiophenol (141 mg) was dissolved in DMF (3 mL) and NaH (39 mg, ca 60% in mineral oil) was added and the mixture was stirred at r.t. After 45 min the mixture was added to 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranosid (187 mg) dissolved in DMF (2 mL) and the resulting mixture was heated to 55 °C. After 60 min the reaction was removed from the heat and allowed to reach r.t. slowly over night. The mixture was diluted with EtOAc and washed four times with brine. The aqueous phase was extracted twice with EtOAc and the combined organic phase was concentrated in vacuo. The residue was purified by flash chromatography (SiO2, 5-70% EtOAc in petroleum ether) to give 87 mg 5-chloro-2-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside. 1H NMR (400 MHz, Chloroform-d) δ 7.50 (dd, J = 6.1, 2.5 Hz, 1H), 7.32 - 7.22 (m, 1H), 7.04 (t, J = 8.7 Hz, 1H), 5.99 (d, J = 5.5 Hz, 1H), 5.53 - 5.45 (m, 1H), 5.29 (dd, J = 10.9, 5.5 Hz, 1H), 4.66 - 4.57 (m, 1H), 4.10 (dd, J = 11.6, 5.0 Hz, 1H), 4.06 - 3.92 (m, 2H), 2.20 (s, 3H), 2.15 (s, 3H), 1.99 (s, 3H).

i64) 5-Bromo-2-fluorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


O-5-bromo-2-fluorophenyl dimethylcarbamothioate



[0591] 



[0592] To a solution of 5-bromo-2-fluoro-phenol (1000 mg, 5.24 mmol) in DMF (10 mL) were added NaH (180.55 mg, 7.85 mmol). After 10 mins, N,N-dimethylcarbamothioyl chloride (970.73 mg, 7.85 mmol) was added. The reaction mixture was stirred at room temperature for 20h. Water (30 mL) and DCM (30 mL) were added. The organic layer was washed with brine, dried (Na2SO4) and evaporated to dryness. The crude products were purified by flash column chromatography to obtain O-(5-bromo-2-fluoro-phenyl) N,N-dimethylcarbamothioate (900 mg, 61.80%) as a white solid.

S-5-bromo-2-fluorophenyl dimethylcarbamothioate



[0593] 



[0594] To a solution of 0-(5-bromo-2-fluoro-phenyl) N,N-dimethylcarbamothioate (900 mg, 3.24 mmol) in phenoxybenzene (8 mL) was held at 280 °C with stirring on for 2 h. The reaction mixture was cooled to room temperature. The crude product was purified by flash column chromatography to obtain S-(5-bromo-2-fluoro-phenyl) N,N-dimethylcarbamothioate (730 mg, 81.11%) as a yellowy solid.

5-bromo-2-fluorobenzenethiol



[0595] 



[0596] To a solution of S-(5-bromo-2-fluoro-phenyl) N,N-dimethylcarbamothioate (730 mg, 2.62 mmol) in methanol (10 mL) was added 10% NaOH aq. (8 ml). The reaction was held at 50 °C with stirring on for 4 h. TLC analysis indicated the total consumption of the SM. The reaction mixture was cooled to room temperature and adjusted pH to 3, then DCM (15 ml) was added. The organic layer was evaporated to dryness and used directly in the next step.

5-bromo-2-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0597] 



[0598] To a solution of 5-bromo-2-fluoro-benzenethiol (664.7 mg, 3.21 mmol) in DMF (10 mL) were added NaH (59.04 mg, 2.57 mmol) at 0 °C under N2. After 10 min, 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (561.31 mg, 1.60 mmol) was added. The reaction mixture was stirred at 50 °C stirring for 4 h. The mixture was diluted with DCM (20 ml) and washed with 10% citric acid (15 ml). The organic layer was evaporated to dryness. The crude product was purified by flash column chromatography to obtain 5-Bromo-2-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (400 mg, 47.90%) as a white solid.

5-Bromo-2-fluorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0599] 



[0600] To a solution of 5-bromo-2-fluorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (200 mg, 0.25 mmol) in DMF (6 mL) were added TEA (194.47 mg, 1.92 mmol), Copper(I)Iodide (21.96 mg, 0.12 mmol), 3,4,5-trifluorophenylacetylene (175.49 mg, 0.77 mmol). The reaction was stirred at 100°C for 2 h. TLC analysis indicated the total consumption of the SM. Water (10 mL) and DCM (20 mL) were added. The aqueous phase was extrated with DCM (20 mL2), and the combined organic phase was washed with brine, dried (Na2SO4) and evaporated to dryness. The crude products were purified by flash column chromatography to give the target compound of 5-Bromo-2-fluorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (170 mg, 65%) as a brown solid.

i65) 5-Chloro-2-methoxyphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside


5-Chloro-2-mehoxythiophenol



[0601] 



[0602] 5-Chloro-2-methoxybenzenesulfonyl chloride (260 mg) was dissolved in THF (2.5 mL) and stirred at r.t. Triphenylphosphine (990 mg) was added followed by water (0.340 mL). After 2 h the mixture was diluted with Et2O and extracted with NaOH (2M). The aqueous phase was washed once with Et2O. The aqueous phase was then acidified to pH 1 with HCl (conc., aq.) and extracted twice with CH2Cl2. The combined organic phase was dried through a phase separator and concentrated to give 175 mg of 5-chloro-2-mehoxythiophenol. 1H NMR (400 MHz, DMSO-d6) δ 7.43 (d, J = 2.5 Hz, 1H), 7.14 (dd, J = 8.7, 2.5 Hz, 1H), 6.97 (d, J = 8.8 Hz, 1H), 5.14 (s, 1H), 3.81 (s, 3H).

5-Chloro-2-methoxyphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0603] 



[0604] 5-Chloro-2-mehoxythiophenol (144 mg) was dissolved in DMF (3 mL) and NaH (36 mg, ca 60% in mineral oil) was added and the mixture was stirred at r.t. After 3 h the mixture was added to 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranosid (130 mg) dissolved in DMF (2 mL) and the resulting mixture was heated to 55 °C. After 75 min the reaction was removed from heat and allow to reach r.t. slowly over night. The mixture was diluted with EtOAc and washed four times with brine. The aqueous phase was extracted twice with EtOAc and the combined organic phase was concentrated in vacuo. The residue was purified by flash chromatography (SiO2, 5-70% EtOAc in petroleum ether) to give 65 mg 5-chloro-2-methoxyphenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside. 1H NMR (400 MHz, Chloroform-d) δ 7.44 (d, J = 2.5 Hz, 1H), 7.23 (dd, J = 8.7, 2.5 Hz, 1H), 6.80 (d, J = 8.8 Hz, 1H), 6.12 (d, J = 5.5 Hz, 1H), 5.46 (d, J = 3.0 Hz, 1H), 5.31 (dd, J = 10.9, 5.5 Hz, 1H), 4.66 - 4.58 (m, 1H), 4.11 - 4.01 (m, 2H), 3.96 (dd, J = 11.5, 7.7 Hz, 1H), 3.86 (s, 3H), 2.19 (s, 3H), 2.16 (s, 3H), 1.96 (s, 3H). ESI-MS m/z calcd for [C19H22ClN3O8SNa]+ (M+Na)+: 510.1; found: 510.0.

i66) 3-Iodophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside


O-(3-Iodophenyl) N,N-dimethylcarbamothioate



[0605] 



[0606] NaH (102 mg, ca 60% in mineral oil) was suspended in DMF (7 mL). 3-Iodophenol (502 mg) was added and the mixture stirred 10 min. N,N-Dimethylthiocarbamoyl chloride (420 mg) was added and the mixture heated to 80 °C 1.5 h. The mixture was allowed to cool, then partitioned between diethyl ether (150 mL) and water (150 mL). The organic phase was washed with water (2x150 mL), aq. sat. NaHCO3 (150 mL) and brine (150 mL), dried (MgSO4), filtered and concentrated in vacuo. The residue was purified by flash chromatography (SiO2, 5-75% EtOAc in heptane). This gave as a white solid (523 mg).

S-(3-Iodophenyl) N,N-dimethylcarbamothioate



[0607] 



[0608] O-(3-iodophenyl) N,N-dimethylcarbamothioate (401 mg) under a argon atmosphere was heated to 260 °C for 1 h. The residue was purified by flash chromatography (SiO2, 2-75% EtOAc in petroleum ether). This afforded a yellow liquid (185 mg).

3-Iodobenzenethiophenol



[0609] 



[0610] S-(3-Iodophenyl) N,N-dimethylcarbamothioate (170 mg) was dissolved in MeOH (20 mL). 2M aq. sodium hydroxide (2.0 mL) was added and the mixture stirred at reflux 3 h. 1M aq. HCl (4 mL) was added and the mixture partitioned between ether (150 mL) and water (150 mL). The organic phase was washed with brine (100 mL), dried (MgSO4) and concentrated in vacuo. This afforded a yellow oil (122 mg). 1H NMR (400 MHz, Chloroform-d) δ 7.69 (t, J = 1.6 Hz, 1H), 7.54 (dt, J = 7.9, 1.2 Hz, 1H), 7.36 - 7.24 (m, 1H), 7.01 (t, J = 7.9 Hz, 1H), 3.51 (s, 1H).

3-Iodophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0611] 



[0612] NaH (50 mg, ca 60% in mineral oil) was suspended in DMF (2 mL). 3-Iodobenzenethiophenol (122 mg) in DMF (4 mL) was added. After 5 min, the thiolate was added to 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (197 mg) in a vial. The mixture was heated to 50 °C 1 h, then stirred at r.t. over night. The mixture was diluted with CH2Cl2 (15 mL) and extracted with water (2x10 mL). The organic phase was concentrated. The residue was purified by flash chromatography (SiO2, 1-50% EtOAc in heptane). This afforded a white solid (103 mg). 1H NMR (400 MHz, Chloroform-d) δ 7.80 (s, 1H), 7.61 (dt, J = 8.3, 1.2 Hz, 1H), 7.44 - 7.38 (m, 1H), 7.03 (dd, J = 8.4, 7.2 Hz, 1H), 5.98 (d, J = 5.5 Hz, 1H), 5.47 (s, 1H), 5.28 (dd, J = 11.0, 5.6 Hz, 1H), 4.62 (dd, J = 7.4, 5.4 Hz, 1H), 4.12 (dd, J = 11.5, 5.2 Hz, 1H), 4.05 - 3.91 (m, 2H), 2.18 (s, 3H), 2.16 (s, 3H), 2.00 (s, 3H).

i68) 3-Cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside


O-3-Cyanophenyl N,N-dimethylthiocarbamate



[0613] 



[0614] 3-Hydroxybenzonitrile (277 mg) was dissolved in DMF (2 mL) and stirred at r.t. NaH (93 mg, ca 60% in mineral oil) was added in portion and the mixture was cooled to 0 °C. After stirring for 10 min, dimethylthiocarbamoyl chloride (390 mg) was added. After 5 min the mixture was removed from cold and heated to 80 °C. After 2 h the mixture was poured into NaHCO3 (sat. aq.) and extracted with EtOAc. The organic phase was washed twice with brine, and the aqueous phase was extracted once with EtOAc. The combined organic phase was dried (Na2SO4) and concentrated. The residue was purified by flash chromatography (SiO2, 5-95% EtOAc in petroleum ether) to give 392 mg O-3-cyanophenyl N,N-dimethylthiocarbamate. 1H NMR (400 MHz, Chloroform-d) δ 7.58 - 7.53 (m, 1H), 7.50 (t, J = 7.9 Hz, 1H), 7.38 (s, 1H), 7.34 (ddt, J = 7.8, 3.1, 1.5 Hz, 1H), 3.47 (s, 3H), 3.37 (s, 3H).

S-3-Cyanophenyl N,N-dimethylthiocarbamate



[0615] 



[0616] O-3-Cyanophenyl N,N-dimethylthiocarbamate. (345 mg) was heated to 220 °C under N2 for 1 h and then 240 °C for 5.5 h. The residue was purified by flash chromatography (SiO2, 5-70% EtOAc in petroleum ether) to give 149 mg S-3-cyanophenyl N,N-dimethylthiocarbamate.

3-Sulfanylbenzonitrile



[0617] 



[0618] S-3-Cyanophenyl N,N-dimethylthiocarbamate (149 mg) was dissolved in MeOH (20 mL) and NaOH (2M, 2.0 mL) was added and the mixture was heated to reflux. After 3.75 h the mixture was neutralized with HCl (1M, aq.), diluted with brine and extracted three times with CH2Cl2. The organic phase was dried through a phase separator and concentrated to give 93 mg 3-sulfanylbenzonitrile. 1H NMR (400 MHz, Chloroform-d) δ 7.55 (s, 1H), 7.49 (d, J = 7.9 Hz, 1H), 7.44 (d, J = 7.7 Hz, 1H), 7.34 (t, J = 7.9 Hz, 1H).

3-Cyanophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0619] 



[0620] 3-Sulfanylbenzonitrile (93 mg) was dissolved in DMF (3 mL) and NaH (29 mg, ca 60% in mineral oil) was added and the mixture was stirred at r.t. After 80 min the mixture was added to 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (125 mg) dissolved in DMF (2 mL) and the resulting mixture was heated to 55 °C. After 65 min the reaction was removed from the heat and allowed to reach r.t. slowly over night. The mixture was diluted with EtOAc and washed four times with brine. The aqueous phase was extracted twice with EtOAc and the combined organic phase was concentrated in vacuo. The residue was purified by flash chromatography (SiO2, 5-75% EtOAc in petroleum ether) to give 79 mg 3-cyanophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside. 1H NMR (400 MHz, Chloroform-d) δ 7.76 (s, 1H), 7.68 (d, J = 8.0 Hz, 1H), 7.57 (d, J = 7.7 Hz, 1H), 7.43 (t, J = 7.8 Hz, 1H), 6.02 (d, J = 5.5 Hz, 1H), 5.49 (s, 1H), 5.28 (dd, J = 10.9, 5.5 Hz, 1H), 4.66 - 4.57 (m, 1H), 4.14 (dd, J = 11.6, 4.9 Hz, 1H), 4.06 - 3.91 (m, 2H), 2.19 (s, 3H), 2.17 (s, 3H), 2.00 (s, 3H).

3-Cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside



[0621] 



[0622] 3-Cyanophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (79 mg) was dissolved in MeCN (5 mL) and stirred at r.t. under N2. Copper(I) iodide (25 mg) was added and after five minutes 3,4,5-trifluorophenylacetylene (0.050 mL) was added. After an additional five minutes DIEA (0.035 mL) was added and the mixture was stirred at r.t. After 18 h the reaction mixture was concentrated in vacuo. The crude product was purified by flash chromatography (SiO2, 5-70% EtOAc in petroleum ether) to give 72 mg 3-cyanophenyl 2,4,6-tri-O-acetyl-3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside. 1H NMR (400 MHz, Chloroform-d) δ 7.81 (d, J = 2.0 Hz, 2H), 7.74 (d, J = 8.0 Hz, 1H), 7.62 (d, J = 7.8 Hz, 1H), 7.46 (dt, J = 12.5, 7.9 Hz, 3H), 6.20 (d, J = 5.6 Hz, 1H), 6.10 (dd, J = 11.6, 5.6 Hz, 1H), 5.63 (d, J = 2.4 Hz, 1H), 5.22 (dd, J = 11.6, 3.1 Hz, 1H), 4.85 (t, J = 6.3 Hz, 1H), 4.17 (dd, J = 11.6, 5.2 Hz, 1H), 4.07 (dd, J = 11.6, 7.4 Hz, 1H), 2.07 (s, 3H), 2.01 (s, 3H), 1.98 (s, 3H). ESI-MS m/z calcd for [C27H24F3N4O7S]+ (M+H)+: 605.1; found: 605.1.

i69) 2-cyanopyridine-5-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


5-mercaptopicolinonitrile



[0623] 



[0624] To a solution of 5-fluoropicolinonitrile (5 g, 40.95 mmol) in DMF (200 mL) was added Na2S•9H2O (14.8 g, 61.43 mmol). The reaction was stirred at room temperature for 20 h. The mixture was added NaHSO4 aq to adjust pH 4-5 and MTBE (100 ml) was added. The organic phase was washed with water (100 mL) and brine (100 mL), dried over anhydrous sodium sulphate. Removal of solvent gave the desired product (4.5 g,crude).
m/z calcd for [C6H4N2S]- [M-H]-: 135.0; found: 135.0.

2-cyanopyridine-5-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0625] 



[0626] To a solution of 5-mercaptopicolinonitrile (4.5 g, 33.09 mmol) in DMF (200 mL) was added NaH (1.1 g, 36.40 mmol) at 0 °C. After 10 min, 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (5.7 g, 16.55 mmol) was added. The mixture was stirred at room temperature for 4 h. Water (200 mL) and DCM (100 mL) were added. The aqueous phase was extrated with DCM (100 mL X 2), the combined organic phase was washed with water (200 mL) and brine (200 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The residue was purified by column chromatography (PE/EtOAc=3/1) to obtain the desired product (2.1 g, 28 %).
m/z calcd for [C18H19N5O7S]+ [M+H]+: 450.0; found: 450.0.

2-cyanopyridine-5-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0627] 



[0628] To a solution of 2-cyanopyridine-5-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (2.1 g, 4.68 mmol) in DMF (20 mL) were added TEA (3.2 mL), Copper(I)Iodide(268 mg, 1.40 mmol), CsF(1.1 g, 7.02 mmol), 3,4,5-trifluorophenylacetylene (1.6 g, 7.02 mmol). The reaction was stirred at room temperature for 20 h under inert atmosphere(N2). Water (30 mL) and DCM (30 mL) were added. The aqueous phase was extrated with DCM (20 mL X 2), the combined organic phases were washed with water (40 mL) and brine (40 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The residue was purified by column chromatography (PE/EtOAc=2/1) to obtain the desired product (1.2 g, 42.85%).
m/z calcd for [C26H22F3N5O7S]+ [M+H]+: 606.0; found: 606.0.
1H NMR (400 MHz, CDCl3) δ 8.77 (d, J = 1.8 Hz, 1H), 7.96 (dd, J = 8.2, 2.3 Hz, 1H), 7.78 (s, 1H), 7.67 (d, J = 8.2 Hz, 1H), 7.44 (dd, J = 8.1, 6.5 Hz, 2H), 6.30 (d, J = 5.6 Hz, 1H), 6.15 (dd, J = 11.6, 5.6 Hz, 1H), 5.62 (d, J = 2.3 Hz, 1H), 5.21 (dd, J = 11.7, 3.1 Hz, 1H), 4.81 - 4.76 (m, 1H), 4.11 (ddd, J = 19.1, 11.7, 6.3 Hz, 2H), 2.08 (s, 3H), 1.98 (d, J = 1.7 Hz, 6H).

i70) 4-Chloro-2-thienyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


4-chlorothiophene-2-thiol



[0629] 



[0630] To a solution of 4,5-dichlorothiophene-2-sulfonyl chloride (2500 mg, 9.94 mmol) in dry toluene (30 mL) in a three-neck round-bottom flask with a nitrogen inlet, reflux condenser, and calcium chloride guard tube, was added Ph3P (7820.5 mg, 29.82 mmol) in portions (CAUTION: reaction is highly exothermic and may start refluxing). The reaction was stirred for 10 min and allowed to cool to below 50 °C.

[0631] Water (10 mL) was added and the mixture was stirred for 10 min. The aqueous layer was discarded and the organic layer was extracted with 10% NaOH (2 × 30 mL). The alkaline aqueous extract was washed with toluene (2 × 20 mL), acidified with dilute HCl and extracted with DCM (2 × 15 mL). The organic layer was dried over Na2SO4 and the solvent was evaporated under reduced pressure to afford 4-chlorothiophene-2-thiol (50 mg, 3.34% yield).

4-Chloro-2-thienyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0632] 



[0633] NaH (7.95 mg, 0.33 mmol, 96% in mineral) was added to a solution of 4-chlorothiophene-2-thiol (50 mg, 0.33 mmol) in DMF (4 mL) at 0 °C. Then it was stirred at 50 °C for 30 min. 2,4,6-tri-O-Acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (94.47 mg, 0.27 mmol) in DMF (1 mL) was added to mixture. The mixture was stirred at 50 °C for 2 h. Then it was cooled to room temperature, water (10 mL) was added to mixture. The resulting solution was extracted with EtOAc (10 mL x 3). The combined organic layers were washed with brine (15 mL), dried over Na2SO4, concentrated in vacuum to give crude product. Purification by flash chromatography afforded the crude product 4-Chloro-2-thienyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (50 mg, 32.47%).
ESI-MS m/z calcd for [C16H18ClN3O7S2]+ (M+H)+: 464.0; found: 464.0.

4-Chloro-2-thienyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0634] 



[0635] 4-Chloro-2-thienyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (50 mg, 0.11 mmol), 3,4,5-trifluorophenylacetylene (36.91 mg, 0.16 mmol), CuI (6.16 mg, 0.03 mmol) and Et3N (54.53 mg, 0.54 mmol) were dissolved in DMF (5 mL) and the mixture was stirred at 50 °C for 2 h. Then it was cooled to room temperature. The mixture was filtered, the filtrated was concentrated in vacuo to afford crude product, which was used for next step without further purification.
ESI-MS m/z calcd for [C24H21ClF3N3O7S2]+ [M+H]+: 619.0; found: 620.0.

i71) (Methyl-1-benzoate)-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


Methyl 3-mercaptobenzoate



[0636] 



[0637] To a solution of 3-sulfanylbenzoic acid (200 mg, 1.30 mmol) in methanol (10 mL) were added H2SO4 (1 ml). The reaction mixture was stirred at 85 °C for 1 h. Water (20 mL) and EtOAc (30 mL) were added. The aqueous phase was extrated with Ethyl acetate (20 mL2), the combined organic phases were washed with brine, dried over Na2SO4, filtered and evaporated to dryness to obtain methyl 3-sulfanylbenzoate.

(Methyl 1-benzoate)-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0638] 



[0639] To a solution of methyl 3-sulfanylbenzoate (200 mg, 1.19 mmol) in DMF (10 mL) were added NaH (21.87 mg, 0.95 mmol). After 0.5h, 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (207.9 mg, 0.59 mmol) was added. The mixture was stirred at room temperature for 2 h. Water (20 mL) and DCM (20 mL) were added. The aqueous phase was extrated with DCM (20 mL2), the combined organic phases were washed with water (30 mL), brine (30 mL), dried over anhydrous sodium sulphate. Solvents were evaporated and the residue was purified by flash column chromatography(PE/EtOAc=3/1) to obtain (Methyl 1-benzoate)-3-yl 2,4,6-triO-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (60 mg, 11%).

(Methyl 1-benzoate)-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0640] 



[0641] To a solution of (Methyl 1-benzoate)-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (60 mg, 0.12 mmol) in DMF (5 mL) were added CuI (7.12 mg, 0.04 mmol), 3,4,5-trifluorophenylacetylene (42.67 mg, 0.19 mmol) and TEA(63.05 mg, 0.62 mmol). The reaction mixture was stirred at 100 °C for 2 h. Water (20 mL) and DCM (20 mL) were added. The aqueous phase was extrated with DCM (20 mL2), the combined organic phase was washed with water (30 mL) and brine (30 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The residue was purified by flash column chromatography(PE/EtOAc=3/1) to obtain (methyl 1-benzoate)-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (30 mg, 38%).

i73) 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


[(3,4-difluorophenyl)-ethynyl]-trimethylsilane



[0642] 



[0643] To a solution of 1,2-difluoro-4-iodo-benzene (3 g, 1 mol) in THF (50 ml) was added ethynyl(trimethyl)silane (2.46 g, 2 mol), iodocopper (475 mg, 0.2 mol), Palladium chloride; triphenylphosphine (878 mg, 0.1mol), N-ethyl-N-isopropyl-propan-2-amine (2.55 g, 0.2 mol). The mixture was purged three times with N2. The mixture was stirred at RT overnight. LC-MS analysis indicated the target compound was formed. The reaction was quenched with water (50 ml). The mixture was extracted with dichloromethane and the aqueous phase was discarded. The mixture was washed with brine (50 ml) and the aqueous phase was discarded. The material was dried over Na2SO4, filtered, and concentrated to dryness. The crude product was purified by combiflash to afford the target compound 350 mg crude of 3.8 g (72.1%). HNMR (500 MHz, CDCl3) δ 7.06-7.07(m, 1H), 6.95-6.70 (m, 1H), 6.87-6.89 (m, 1H), 0.69 (s, 9H).

3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0644] 



[0645] To a solution of 3,4-dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (200 mg, 4.31 mmol) in N,N-dimethylformamide (10 ml) was added [(3,4-difluorophenyl)-ethynyl]-trimethylsilane (246.67 mg, 8.62 mmol), N,N-diethylethanamine (40 mg, 4.31 mmol), copper iodide (22 mg, 1.29 mmol). The mixture was purged three times with N2. The mixture was stirred at 100°C for 30 min. LC-MS analysis indicated the target compound was formed. The reaction was quenched with water(50 ml). The mixture was extracted with dichloromethane and the aqueous phase was discarded. The mixture was washed with brine (50 ml) and the aqueous phase was discarded. The material was dried over Na2SO4, filtered, and concentrated to dryness. The crude product was purified by combiflash (EtOAc:PE=1:3 to 3:1 ISCO 40 g 40 ml/min normal phase sillica, uv254) to afford the target compound 250 mg.
ESI-MS m/z calcd for [C26H23Cl2F2N3O7S]+ [M+H]+: 630.0; found: 630.0.

i74) 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0646] 



[0647] 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (150 mg, 0.3 mmol), 3,5-difluorophenylacetylene (90 mg, 0.61 mmol), Copper(I)Iodide (20 mg, 0.09 mmo) and Triethylamine (154 mg, 1.52 mmol) were added to a flask. DMF (3 mL) was added. The mixture was stirred at 100 °C for 1 hour under inert atmosphere (N2). The mixture was filtered and concentracted in vacuum to give 160 mg of the title compound.
ESI-MS m/z calcd for [C26H24Cl2F2N3O7S]+ (M+H)+: 630.1; found: 630.0.

i75) 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3-fluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0648] 



[0649] 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (150 mg, 0.3 mmol), 1-ethynyl-3-fluoro-benzene (109.8 mg, 0.91 mmol), Copper(I)Iodide (17.41 mg, 0.09 mmol) and Triethylamine (154.15 mg, 1.52 mmol) was dissolved in DMF (5 mL). The mixture was stirred at 100 °C for 1 hour. LCMS showed that the product was formed and no SM left. EtOAc (200 ml) was added. The mixture was filtered, washed by water (100 mL), dried by Na2SO4, filtered and concentrated. The residue was purified on silica gel column to give 140 mg (75%) of the title compound.
ESI-MS m/z calcd for [C26H25Cl2FN3O7S]+ (M+H)+: 612.1; found: 612.1.

i76) 3,3'-difluoro-cyclohexyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


1,2,4,6-tetra-O-acetyl-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-3-deoxy-β-D-galactopyranoside



[0650] 



[0651] To a solution of 1,2,4,6-tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (2.00 g, 5.36 mmol) in DMF (20 mL) were added TEA (2.71 g, 26.80 mmol), Copper(I)Iodide (306.2 mg, 1.61 mmol), 3,4,5-trifluorophenylacetylene (1.83 g, 8.04 mmol). The reaction was held at 100 °C with stirring on for 2 h under N2. Water (80 mL) and DCM (80 mL) were added. The aqueous phase was extracted with DCM (10 mL2), the combined organic phase was washed with water (20 mL) and brine (20 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The residue was purified by column chromatography(PE/EtOAc=2/1) to obtain the title compound (2 g, 71%).

2,4,6-tri-O-acetyl-1-chloro-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-β-D-galactopyranoside



[0652] 



[0653] To a stirred suspension of 1.9 g, 3.78 mmol) and PCl5 (1.56 g, 7.56 mmol) in dry DCM (20 mL), BF3·Et2O (2 mL) was added. After stirring for 2 h, TLC analysis showed complete disappearance of the starting material. The reaction mixture was diluted with DCM (50 mL) and then washed with ice-cold water, sat. ice-cold NaHCO3 solution (2×50 mL), and again ice-cold water successively, dried over anhydrous Na2SO4 and evaporated under reduced pressure to afford the crude product 1 g as a white solid. This was used directly in the next step.

Acetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0654] 



[0655] To a solution of 2,4,6-tri-O-acetyl-1-chloro-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-β-D-galactopyranoside (1.00 g, 1.98 mmol) in DMF (10 mL) was added potassium thioacetate (452.3 mg, 3.96 mmol). The reaction mixture was stirred at room temperature for 20 h. Water (40 mL) and DCM (40 mL) were added. The aqueous phase was extrated with DCM (40 mL2), the combined organic phase was washed with water (50 mL) and brine (50 mL), dried over anhydrous sodium sulphate and evaporated to dryness. The crude products were purified by flash column chromatography (PE/EtOAc=3/2) to obtain the title compound (450 mg, 42%).

3-oxo-cyclohexyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0656] 



[0657] To a solution of acetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (450 mg, 0.83 mmol) in DCM (10 mL) were added cyclohex-2-en-1-one (158.7 mg, 1.66 mmol) and diethylamine(1.21 g, 16.6 mmol). The reaction mixture was stirred at room temperature for 2h. Water (30 mL) and DCM (30 mL) were added.The aqueous phase was extrated with DCM (30 mL2), the combined organic phase was washed with brine, dried (Na2SO4) and evaporated to dryness to obtain the title compound (200 mg, 40 %).

3,3'-difluoro-cyclohexyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0658] 



[0659] To a solution of 3-oxo-cyclohexyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (60 mg, 0.10 mmol) in DCM (5 mL) were added DAST (80.7 mg, 0.50 mmol). The reaction was stirred at 35 °C for 2 h. The crude product was purified by preparpative-TLC to obtain the title compound (30 mg, 48%).

i77) n-Butyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


n-Butyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0660] 



[0661] To a solution of 1,2,4,6-tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (373 mg, 1.0 mmol) in CHCl3 (10 mL) were added butane-1-thiol (79.38 mg, 0.88 mmol), followed by BF3·OEt2 (1419.3 mg, 10.0 mmol). Reaction was allowed to proceed at room temperature in dark room for 1 h. Excess BF3·OEt2 was decomposed by the addition of cold water (10 mL), and the mixture was then extracted with EtOAc (10 mL X 3). The combined organiclayerss were dried over Na2SO4 and concentracted in vacuo to afford crude product afford the title compound (380 mg, 94% yield).
ESI-MS m/z calcd for [C16H25N3O7S]+ [M+H]+: 403.1; found: 404.2.

n-Butyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0662] 



[0663] To a solution of n-Butyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (380 mg, 0.94 mmol) in MeCN (10 mL) was added 3,4,5-trifluorophenylacetylene (321.5 mg, 1.41 mmol), iodocopper (53.3 mg, 0.28 mmol), CsF (214.2 mg, 1.41 mmol) and Et3N (475.6 mg, 4.7 mmol). The reaction vessel was purged 3 times with nitrogen. Then the mixture was stirred at room temperature for 2 h. The mixture was extracted with EtOAc (10 mL X 3). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product, which was purified by flash chromatography to afford the title compound (300 mg, 57.0% yield). 1H NMR (400 MHz, MeOD) δ 8.36 (s, 1H), 7.46 (dd, J = 8.7, 6.7 Hz, 2H), 5.80 (dt, J = 8.1, 5.6 Hz, 2H), 5.42 (d, J = 2.3 Hz, 1H), 5.23 (d, J = 3.0 Hz, 1H), 5.21 (d, J = 3.2 Hz, 1H), 4.68 (t, J = 6.4 Hz, 1H), 4.01 (qd, J = 11.4, 6.4 Hz, 2H), 2.51 (dtd, J = 20.2, 12.9, 7.3 Hz, 2H), 1.88 (s, 6H), 1.73 (s, 3H), 1.55 - 1.40 (m, 2H), 1.39 - 1.20 (m, 2H), 0.81 (t, J = 7.3 Hz, 3H).
ESI-MS m/z calcd for [C24H28F3N3O7S]+ (M+H)+: 559.2; found: 560.2.

i78) 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,5-difluoro-4-methoxyphenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


((3,5-difluoro-4-methoxyphenyl)ethynyl)trimethylsilane



[0664] 



[0665] The mixture of 5-bromo-1,3-difluoro-2-methoxy-benzene (2.6 g, 11.7 mmol), ethynyl (trimethyl)silane (1.145g, 11.7 mmol), N,N-Diisopropylethylamine (1.205 g, 9.33 mmol), Copper(I)Iodide (177.6 mg, 0.93 mmol) and Bis(Triphenylphosphine)palladium (II) chloride (340.4 mg, 0.47 mmol) in THF (20 mL) were heated at 60 °C under a nitrogen atmosphere for 16 hours. Added water (50 mL) and extracted by EtOAc (60 mL), washed EtOAc phase with water (50 mL), brine (50 mL), dried over Na2SO4, filtered and concentrated to give a residue. The residue was purified by slica gel column (pure PE) to afford the title compound as an oil.

3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,5-difluoro-4-methoxyphenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0666] 



[0667] 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (150 mg, 0.3 mmol), 2-(3,5-difluoro-4-methoxy-phenyl)ethynyl-trimethyl-silane (219.66 mg, 0.91 mmol), Copper(I)Iodide (17.41 mg, 0.09 mmol) and triethylamine (154.15 mg, 1.52 mmol) was dissolved in DMF (5 mL). The mixture was stirred at 100°C for 1 hour. LCMS showed that the product was formed and no SM left. EtOAc (50 mL) was added. The mixture was filtered, washed by water (100 mL), dried by Na2SO4, filtered and concentrated. The residue was purified on silica gel column to afford the title compound (120 mg, 59.6% yield).
ESI-MS m/z calcd for [C27H25C12F2N3O8S]+ (M+H)+: 659.1; found: 660.1.

i79) 2-hydroxy-pyridin-4-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


O-2-hydroxypyridin-4-yl dimethylcarbamothioate



[0668] 



[0669] To a solution of pyridine-2,4-diol (2.2 g, 0.02 mol) in THF (20 mL), Dabco (2.22 g, 0.02 mol) and N,N-dimethylcarbamothioyl chloride (2.45 g, 0.02 mol) were added.

[0670] The mixture was stirred at rt for 16 h. The solvents were removed in vacuo and the resulting crude was diluted with CH2Cl2 and washed with water and brine. The organic layer was dried over Na2SO4, filtered and evaporated. The crude product was purified by silica gel column to give 0.9 g (22.9%) of the title compound.
ESI-MS m/z calcd for [C8H11N2O2S]+ (M+H)+: 199.1; found: 199.0.

S-1-(4-methoxybenzyl)-2-oxo-1,2-dihydropyridin-4-yl dimethylcarbamothioate



[0671] 



[0672] To a solution of O-[(2-hydroxy-4-pyridyl)] N,N-dimethylcarbamothioate (400 mg) in DMF (6 mL), NaH (60%, 0.15 g) was added at 0°C. The mixture was stirred at 0°C for 15 min. 1-(bromomethyl)-4-methoxy-benzene (1.22 g, 0.01 mol) was added. The mixture was stirred at rt for 2 hours. EtOAc (100 ml) was added. the mixture was washed by water (40 mL), brine (40 mL), dried over Na2SO4, filtered and concentrated. The residue was purified on silica gel column. 0.4 g (62.3%) of the title compound was obtained.
ESI-MS m/z calcd for [C16H19N2O3S]+ (M+H)+: 319.1; found: 319.1.

S-1-(4-methoxybenzyl)-2-oxo-1,2-dihydropyridin-4-yl dimethylcarbamothioate



[0673] 



[0674] S-[[1-[(4-methoxyphenyl)methyl]-2-oxo-4-pyridyl]] N,N-dimethylcarbamothioate (0.3 g) was dissloved in phenoxybenzene (3 mL). The mixture was stirred at 280°C for 2 hours. LCMS showed that the desired product was formed and no SM left. The mixture was purified by flash chromatography on silica to give 0.25 g (83.3%) of the title compound.
ESI-MS m/z calcd for [C16H19N2O3S]+ (M+H)+: 319.1; found: 319.1.

4-mercapto-1-(4-methoxybenzyl)pyridin-2(1H)-one



[0675] 



[0676] To a solution of S-[[1-[(4-methoxyphenyl)methyl]-2-oxo-4-pyridyl]] N,N-dimethylcarbamothioate (0.25 g) in EtOH (10 mL), KOH (0.09 g) was added. The mixture was stirred at 85°C for 2 hours. The mixture was cooled to rt. Neutralization with H+ resin, filtered and concentrated to afford product as an oil. The residue was used for next step.
ESI-MS m/z calcd for [C13H14NO2S]+ (M+H)+: 248.1; found: 248.0.

1-(4-methoxybenzyl)-2-oxo-1,2-dihydropyridin-4-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0677] 



[0678] To a solution of 1-[(4-methoxyphenyl)methyl]-4-sulfanyl-pyridin-2-one (0.25 g) in DMF (3 mL), NaH (60%, 0.12 g) was added. The mixture was stirred at rt for 15 min. 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (350 mg) was added. the mixture was stirred at rt for 2 hours. EtOAc (100 mL) was added. The mixture was washed by water (50 mL), brine (50 mL), dried over Na2SO4, filtered and concentrated. The residue was purified on silica gel column. 0.3 g (53.5%) of title compound was obtained.
ESI-MS m/z calcd for [C25H29N4O9S]+ (M+H)+: 561.2; found: 561.2.

1-(4-methoxybenzyl)-2-oxo-1,2-dihydropyridin-4-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0679] 



[0680] 1-(4-methoxybenzyl)-2-oxo-1,2-dihydropyridin-4-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (0.32 g) was dissolved in CH3CN (6 ml). Then CuI (0.03 g), 3,4,5-trifluorophenylacetylene (0.13 g), and DIEA (0.22 g) were added. The mixture was stirred at rt for 5 min. CsF (0.09 g) was added. The mixture was stirred at rt over night. 0.12 g (29.6%) of the title compound was obtained.
ESI-MS m/z calcd for [C33H32F3N4O9S]+ (M+H)+: 717.2; found: 717.2.

2-hydroxy-pyridin-4-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0681] 



[0682] 1-(4-methoxybenzyl)-2-oxo-1,2-dihydropyridin-4-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (100 mg, 0.14 mmol) was dissloved in TFA (5 mL). The mixture was stirred at in a single node microwave oven at 110 °C for 2 hours. The TFA was removed in vacuum. The residue was used for next step.
ESI-MS m/z calcd for [C25H24F3N4O8S]+ (M+H)+: 597.1; found: 597.1

i80) 2-chlorophenetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


2-chlorophenetyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0683] 



[0684] Acetyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (0.5 g) was dissolved in DMF (4 mL). 1-(2-bromoethyl)-2-chloro-benzene (0.33 g) was added. diethylamine (2 mL) was added at 0 °C. The mixture was stirred at 0 °C for 2 hours. EtOAc (200 mL) was added. The mixture was washed by water (100 mL). The organic phase was dried over Na2SO4, filtered and concentrated. The residue was purified on silica gel column to give 0.3 g (48 %) of the title compound.
1H NMR (400 MHz, CDCl3) δ 7.39 - 7.31 (m, 1H), 7.24 - 7.12 (m, 3H), 5.76 (d, J = 5.6 Hz, 1H), 5.42 (d, J = 2.5 Hz, 1H), 5.22 (dd, J = 10.9, 5.6 Hz, 1H), 4.51 (t, J = 6.7 Hz, 1H), 4.05 - 3.88 (m, 2H), 3.05 - 2.94 (m, 2H), 2.91 - 2.71 (m, 2H), 2.16 (s, 3H), 2.14 (s, 3H), 1.98 (s, 3H). ESI-MS m/z calcd for [C20H24CN3O7SNa]+ (M+Na)+: 508.1; found: 508.0.

2-chlorophenetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0685] 



[0686] 2-chlorophenetyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (350 mg, 0.72 mmol) and 3,4,5-trifluorophenylacetylene (164.4 mg, 0.72 mmol) were dissolved in CH3CN (20 mL). Then Copper(I)Iodide (137.2 mg, 0.72 mmol), TEA (72.9 mg, 0.72 mmol) and CsF (109.4 mg, 0.72 mmol) were added. The mixture was stirred at rt over night. The mixture was concentrated and the residue was purified on silica gel using a gradient of EtOAc/PE from 0-30% to give 120 mg (25.9%) of the title compound.
ESI-MS m/z calcd for [C28H28ClF3N3O7S]+ (M+H)+: 642.1; found: 642.1.

i81) 4-Chlorophenetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


Acetyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0687] 



[0688] 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (3 g, 8.58 mmol) was dissolved in DMF (8 mL). Potassium thioacetate (1469.5 mg, 12.87 mmol) was added. The mixture was stirred at rt for 16 hours. EtOAc (200 mL) was added. The mixture was washed with water (100 mL) and brine. The organic phase was dried over Na2SO4, filtered and concentrated. The residue was purified on silica gel column using a gradient of EtOAc/PE from 0-35%. 1.2 g of the title compound was obtained.
ESI-MS m/z calcd for [C14H23N4O8S]+ (M+NH4)+: 407.1; found: 407.2.

4-Chlorophenetyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0689] 



[0690] Acetyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (400 mg, 1.03 mmol) was dissolved in DMF (4 mL). 1-(2-bromoethyl)-4-chloro-benzene (225.5 mg, 1.03 mmol) was added. Diethylamine (2 ml) was added at 0 °C. The mixture was stirred at 0 °C for 2 hours. EtOAc (200 mL) was added. The mixture was washed by water (100 mL). The organic phase was dried over Na2SO4, filtered and concentrated. The residue was purified on silica gel column to give 300 mg (60.10 %) of the title compound.
1H NMR (400 MHz, CDCl3) δ 7.35-7.2 (m, 2H), 7.15 - 7.06 (m, 2H), 5.75 (d, J = 5.6 Hz, 1H), 5.41 (d, J = 2.5 Hz, 1H), 5.19 (dd, J = 10.8, 5.6 Hz, 1H), 4.46 (t, J = 6.3 Hz, 1H), 4.11 (dd, J = 11.5, 5.6 Hz, 1H), 4.02 (dd, J = 11.5, 7.2 Hz, 1H), 3.90 (dd, J = 10.9, 3.3 Hz, 1H), 2.96 - 2.76 (m, 3H), 2.76 - 2.63 (m, 1H).
ESI-MS m/z calcd for [C20H24ClN3O7SNa]+ (M+Na)+: 508.1; found: 508.0.

4-Chlorophenetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0691] 



[0692] 4-Chlorophenetyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (350 mg, 0.72 mmol) and 3,4,5-trifluorophenylacetylene (164.42 mg, 0.72 mmol) were dissolved in CH3CN (20 mL). Then Copper(I)Iodide (137.17 mg, 0.72 mmol), TEA (72.88 mg, 0.72 mmol) and CsF (109.41 mg, 0.72 mmol) were added. The mixture was stirred at rt over night. The mixture was concentrated and the residue was purified on silica gel using a gradient of EtOAc/PE from 0-30% to give 200 mg (43.2%) of the title compound.
ESI-MS m/z calcd for [C28H28ClF3N3O7S]+ (M+H)+: 642.1; found: 642.1.

i82) 2-Chlorobenzyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0693] 



[0694] 1,2,4,6-Tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (287 mg) was dissolved in dichloroethane (10 mL). 2-Chlorobenzylmercaptane (0.200 mL) and BF3•OEt2 (0.400 mL) were added. The vial was closed and heated to 65 °C over night. It was allowed to cool, then washed with water (15 mL). The organic phase was purified by flash chromatography (SiO2, 1-50% EtOAc in heptane). This afforded 2-chlorobenzyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (132 mg). 1H NMR (400 MHz, Chloroform-d) δ 7.41 - 7.34 (m, 1H), 7.30 (dt, J = 8.1, 3.9 Hz, 1H), 7.28 - 7.16 (m, 2H), 5.63 (d, J = 5.6 Hz, 1H), 5.42 (d, J = 3.3 Hz, 1H), 5.19 (dd, J = 10.9, 5.6 Hz, 1H), 4.53 (t, J = 6.5 Hz, 1H), 4.11 (dd, J = 11.4, 5.9 Hz, 1H), 4.02 - 3.76 (m, 5H), 2.13 (d, J = 1.0 Hz, 3H), 2.06 (s, 3H), 2.05 (d, J = 1.2 Hz, 3H).

i83) 3,4-Dichlorobenzyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0695] 



[0696] 1,2,4,6-Tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (229 mg) was dissolved in dichloroethane (10 mL). 3,4-Dichlorobenzylmercaptane (0.200 mL) and BF3•OEt2 (0.400 mL) were added. The vial was closed and heated to 65 °C over night. The mixture was allowed to cool, then washed with water (15 mL). The organic phase was collected and purified by flash chromatography (SiO2, 1-50% EtOAc in heptane). This afforded 3,4-dichlorobenzyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (123 mg) as a clear oil. 1H NMR (400 MHz, Chloroform-d) δ 7.41 - 7.32 (m, 2H), 7.11 (dd, J = 8.3, 2.1 Hz, 1H), 5.51 (d, J = 5.6 Hz, 1H), 5.46-5.40 (m, 1H), 5.18 (dd, J = 10.8, 5.6 Hz, 1H), 4.47 (t, J = 6.4 Hz, 1H), 4.12 (dd, J = 11.5, 5.7 Hz, 1H), 3.98 (dd, J = 11.5, 7.0 Hz, 1H), 3.91 (dd, J = 10.9, 3.3 Hz, 1H), 3.70 - 3.56 (m, 2H), 2.14 (d, J= 0.9 Hz, 3H), 2.08 (s, 3H), 2.06 (s, 3H).

i84) 3-Chlorophenetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


3-Chlorophenetyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0697] 



[0698] 2,4,6-tri-o-acetyl-1-(acetylthio)-3-azido-1,3-dideoxy-α-D-galactopyranoside (0.5 g mol) was dissolved in DMF (4 mL). 2-(3-chlorophenyl)ethanethiol (0.33 g) was added diethylamine (2 ml) was added at 0 °C. The mixture was stirred at 0 °C for 2 hours. EtOAc (200 mL) was added. The mixture was washed by water (100 mL). The organic phase was dried over Na2SO4, filtered and concentrated. The residue was purified on silica gel column to give 0.3 g (48.1%) of the title compound.
1H NMR (400 MHz, CDCl3) δ 7.26 - 7.16 (m, 3H), 7.10 - 7.04 (m, 1H), 5.75 (d, J = 5.6 Hz, 1H), 5.41 (d, J = 2.6 Hz, 1H), 5.20 (dd, J = 10.8, 5.6 Hz, 1H), 4.48 (t, J = 6.4 Hz, 1H), 4.22 - 4.08 (m, 2H), 4.05 (d, J = 7.3 Hz, 1H), 3.91 (dd, J = 10.9, 3.4 Hz, 1H), 2.97 - 2.78 (m, 3H), 2.78 - 2.68 (m, 1H), 2.16 (s, 3H), 2.14 (s, 3H), 2.00 (s, 3H). ESI-MS m/z calcd for [C20H24ClN3O7SNa]+ (M+Na)+: 508.1; found: 508.0.

3-Chlorophenetyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0699] 



[0700] 3-Chlorophenetyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (350 mg, 0.72 mmol) and 3,4,5-trifluorophenylacetylene (164.42 mg, 0.72 mmol) were dissolved in CH3CN (20 mL). Then Copper(I)Iodide (137.17 mg, 0.72 mmol), TEA (72.88 mg, 0.72 mmol) and CsF (109.41 mg, 0.72 mmol) were added. The mixture was stirred at rt over night. The mixture was concentrated and the residue was purified on silica gel using a gradient of EtOAc/PE from 0-30% to give 130 mg (28.1%) of the title compound.
ESI-MS m/z calcd for [C28H28ClF3N3O7S]+ (M+H)+: 642.1; found: 642.1.

i85 4-Chlorobenzyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0701] 



[0702] 1,2,4,6-Tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (181 mg) was dissolved in dichloroethane (10 mL). 4-Chlorobenzylmercaptane (0.150 mL) and BF3•OEt2 (0.200 mL) were added. The vial was closed and heated to 65 °C over night. Allowed to cool, then concentrated in vacuo. Pyridine (10 mL) and acetic anhydride (1 mL) were added, and the mixture then stirred 1 h. It was again concentrated in vacuo. The residue was purified by flash chromatography (SiO2, 1-50% EtOAc in heptane). 4-Chlorobenzyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside was isolated as a clear oil (58 mg). 1H NMR (400 MHz, Chloroform-d) δ 7.28 (d, J = 8.3 Hz, 2H), 7.21 (d, J = 8.3 Hz, 2H), 5.52 (d, J = 5.6 Hz, 1H), 5.43 (d, J = 3.3 Hz, 1H), 5.19 (dd, J = 10.8, 5.6 Hz, 1H), 4.50 (t, J = 6.4 Hz, 1H), 4.12 (dd, J = 11.4, 5.7 Hz, 1H), 3.99 (dd, J = 11.5, 7.1 Hz, 1H), 3.92 (dd, J = 10.8, 3.4 Hz, 1H), 3.73 - 3.60 (m, 2H), 2.14 (s, 3H), 2.08 (s, 3H), 2.07 (s, 3H).

186) Propyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


Propyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0703] 



[0704] To a solution of 1,2,4,6-tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranoside (150 mg, 0.40 mmol) in CHCl3 (10 mL) were added propane-1-thiol (28 mg, 0.35 mmol), followed by BF3·OEt2 (568 mg, 4.0 mmol). Reaction was allowed to proceed at room temperature in a dark room for 1 h. Excess BF3·OEt2 was decomposed by the addition of cold water (10 mL), and the mixture was then extracted with EtOAc (10 mL X 3).

[0705] The combined organic layers were dried over anhyd Na2SO4 and concentracted in vacuo to afford crude product. (150 mg, 96% yield).
ESI-MS m/z calcd for [C15H23N3O7S]+ [M+NH4]+: 389.1; found: 407.2.

Propyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0706] 



[0707] To a solution of propyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (150 mg, 0.39 mmol) in MeCN (10 mL) was added 3,4,5-trifluorophenylacetylene (135 mg, 0.59 mmol), iodocopper (23 mg, 0.12 mmol), CsF (89 mg, 0.59 mmol) and Et3N (197 mg, 1.95 mmol). The reaction vessel was purged 3 times with nitrogen. Then the mixture was stirred at room temperature for 2 h. The mixture was extracted with EtOAc (10 mL X 3). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product, which was purified by flash chromatoigraphy to afford the title compound (100 mg, 47% yield).
ESI-MS m/z calcd for [C23H26F3N3O7S]+ (M+H)+: 545.5; found: 546.2.

i87) 2-Aminopyridin-4-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


2-nitropyridine-4-thiol



[0708] 



[0709] 4-chloro-2-nitro-pyridine (5000 mg, 31.54 mmol) and NaHS (2122 mg, 37.84 mmol) were dissolvent in DMF (50 mL). Then it was stirred at 50°C overnight. Aq NaOH (50 mL) was added to the mixture. The mixture was extracted with EtOAc (10 mL X 3). The aqueous was acidified with aq NaHSO4 to PH ∼ 4. Then it was extracted with EtOAc (15 mL X 3). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product, which was purified by washed with EtOAc (10 mL) to afford 2-nitropyridine-4-thiol(1700 mg, 35% yield). ESI-MS m/z calcd for [C5H4N2O2S]- [M-H]-: 156.0; found: 155.0.

2-Nitropyridin-4-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0710] 



[0711] K2CO3 (708 mg, 5.12 mmol) was added to a solution of 2-nitropyridine-4-thiol (800 mg, 5.12 mmol) in DMF (15 mL) at 0 °C. The solution was stirred at rt for 30 min. Then 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (1433 mg, 4.1 mmol) was added to the mixture. The reaction was stirred at 50 °C overnight. The mixture was extracted with EtOAc (10 mL X 3). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product, which was purified by FLASH CHROMATOGRAPHY to afford the title compound(450 mg, 19 % yield).
ESI-MS m/z calcd for [C17H19N5O9S]+ [M+H] +: 469.1; found: 470.1.

2-nitropyridin-4-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0712] 



[0713] To a solution of 2-nitropyridin-4-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (450 mg, 0.96 mmol) in MeCN (10 mL) was added 3,4,5-trifluorophenylacetylene (328 mg, 1.44 mmol), Copper iodide (54.8 mg, 0.29 mmol), CsF (218 mg, 1.44 mmol) and Et3N (485 mg, 4.79 mmol). The reaction vessel was purged thrice with nitrogen. Then the mixture was stirred at room temperature for 3 h. The mixture was extracted with EtOAc (10 mL X 3). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product, which was purified by flash chromatography to afford the title compound (175 mg, 29% yield).
ESI-MS m/z calcd for [C25H22F3N5O9S]+ (M+H)+: 625.1.0; found: 626.2.

2-Aminopyridin-4-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0714] 



[0715] SnCl2·2H2O (189.4 mg, 0.84 mmol) was added into a solution of 2-nitropyridin-4-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (175 mg, 0.28 mmol) in EtOAc (10 mL). The solution was stirred at 80 °C for 3 h. Water (20 mL) was added to the mixture, then it was basified with aq NaHCO3. The mixture was extracted with EtOAc (10 mL X 3). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product, which was purified by flash chromatography to afford 1,3-dideoxy-2,4,6-tri-O-acetyl-1-(2-aminopyridin-4-ylthio)-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside (124 mg, 74% yield).
ESI-MS m/z calcd for [C25H24F3N5O7S]+ (M+H)+: 595.1.0; found: 596.2.

i88) 5-Dimethylamino-naphatlen-2-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


6-(tert-butyldimethylsilyloxy)naphthalen-1-amine



[0716] 



[0717] A solution of 5-aminonaphthalen-2-ol (500 mg, 3.14 mmol) and imidazole (213.8 mg, 3.14 mmol) in DCM (10 ml) was added dropwise a solution of TBDMS-Chloride (710.1 mg, 4.71 mmol) in DCM (10 mL) with stirring at 10 °C. The reaction mixture was stirred at r.t over 2h followed by addition of water (30ml). The phases were separated and the organic layer was washed with brine, dried over (Na2SO4) and the solvents where evaporated to give crude material.
ESI-MS m/z calcd for [C16H23NOSi]+ (M+H)+: 274.0; found: 274.0.

6-(tert-butyldimethylsilyloxy)-N,N-dimethylnaphthalen-1-amine



[0718] 



[0719] A solution of 6-[tert-butyl(dimethyl)silyl]oxynaphthalen-1-amine (800 mg, 2.93 mmol) and K2CO3 (2021.7 mg, 2.93 mmol) in acetone (20 ml) was added methyl iodide (1245.7 mg, 2.93 mmol) dropwise at 10 °C. The reaction mixture was stirred at reflux for 24 h, followed by additon of water (30ml). The phases where separated and the organic layer was washed with brine, dried over (Na2SO4) and the solvents where evaporated.
ESI-MS m/z calcd for [C18H27NOSi]+ (M+H)+: 302.0; found: 302.0.

5-(dimethylamino)naphthalen-2-ol



[0720] 



[0721] To a stirred solution of 6-[tert-butyl(dimethyl)silyl]oxy-N,N-dimethyl-naphthalen-1-amine (750 mg, 2.49 mmol) in tetrahydrofuran (20 ml) was added TBAF (2355 mg, 7.46 mmol) at rt. The reaction mixture was stirred at r.t over 24 h followed by evaporation of the solvents to give crude material.
ESI-MS m/z calcd for [C12H13NO]+ (M+H)+: 188.0; found: 188.0.

O-5-(dimethylamino)naphthalen-2-yl dimethylcarbamothioate



[0722] 



[0723] To a stirred solution of 5-(dimethylamino)naphthalen-2-ol (1000 mg, 5.34 mmol) and 1,4-diazabicyclooctane (1795 mg, 16.02 mmol) in tetrahydrofuran (30 ml) was added N,N-dimethylcarbamothioyl chloride (1320 mg, 10.68 mmol) at rt. The reaction mixture was stirred at r.t over 16 h. The reaction mixture was evaporated to dryness and purified by biotage flash (PE : EtOAc =10% - 50%) to give 800 mg (54.6%) of the title compound .
ESI-MS m/z calcd for [C15H18N2OS]+ (M+H)+: 275.0; found: 275.0.

S-5-(dimethylamino)naphthalen-2-yl dimethylcarbamothioate



[0724] 



[0725] A solution of O-[[5-(dimethylamino)-2-naphthyl]] N,N-dimethylcarbamothioate (700 mg, 2.55 mmol)) in Phenyl ether (5 ml) was stirred at 250 °C over 2 h. The reaction mixture was purified by column chromatography on silica gel eluted with PE/EtOAc (100:0∼70:30) to give 600 mg (85.7%) of the title compound.
ESI-MS m/z calcd for [C15H18N2OS]+ (M+H)+: 275.0; found: 275.0.

5-(dimethylamino)naphthalene-2-thiol



[0726] 



[0727] S-[[5-(dimethylamino)-2-naphthyl]] N,N-dimethylcarbamothioate (700 mg, 2.55 mmol) and NaOH (408.16 mg, 10.2 mmol) was taken up in 16 mL THF/MeOH/water = 1:1:1 and heated at reflux for 2h. TLC analysis indicated the total consumption of the starting material. The mixture was concentrated to about 10 mL, taken up to 50mL EtOAc, added with 2M hydrochloric acid to adjust the pH to about 6. The organic layer was dried over Na2SO4, concentrated to afford the target compound 500 mg (96%) of the title compound as a yellow oil which was used immediately in the next step.

5-Dimethylamino-naphatlen-2-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0728] 



[0729] NaH (56.5 mg, 2.46 mmol) was suspended in DMF (20 ml) followed by addition of 5-(dimethylamino)naphthalene-2-thiol (500 mg, 2.46 mmol). Then 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (860.1 mg, 2.46 mmol) was added. The mixture was stirred at rt over 2h. The reaction mixture was diluted with CH2Cl2 (DCM) (50 ml), 0.5M citric acid (50 ml) and water (50 ml). The phases were separated and the organic phase was washed with water (10 ml) and concentrated in vacuo. The residue was purified by column chromatography (SiO2/PE:EtOAc 10:1 = 3:1) to give 910 mg (72%) of the title compound as a white solid.
ESI-MS m/z calcd for [C24H28N4O7S]+ (M+H)+: 517.0; found: 517.0.

5-Dimethylamino-naphatlen-2-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0730] 



[0731] A mixture of 5-Dimethylamino-naphatlen-2-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (0.91 g) and 3,4,5-trifluorophenylacetylene (0.4 g) were dissloved in CH3CN (20 ml). Then CsF (0.09 g), and DIEA (0.5 g) were added. The mixture was stirred at rt for 5 min followed by addition of Copper iodide (0.07 g) was added. The mixture was stirred at rt over night. Then the mixture was concentrated and purified by column chromatography (SiO2/PE:EtOAc 10:1 = 3:1) to give 810 mg (68.3%) of the title compound.
1H NMR (400 MHz, CDC13) δ 8.20 (d, J = 8.9 Hz, 1H), 7.96 (d, J = 1.6 Hz, 1H), 7.80 (s, 1H), 7.60 - 7.35 (m, 5H), 7.26 (s, 1H), 7.13 - 7.03 (m, 1H), 6.25 (d, J = 5.5 Hz, 1H), 6.11 (dd, J = 11.6, 5.5 Hz, 1H), 5.63 (d, J = 2.3 Hz, 1H), 5.32 (dd, J = 11.6, 3.1 Hz, 1H), 4.96 (t, J = 6.4 Hz, 1H), 4.23 - 3.99 (m, 2H), 2.89 (s, 6H), 2.06 (s, 3H), 1.99 (s, 3H), 1.81 (s, 3H)
m/z calcd for [C32H31F3N4O7S]+ [M+H]+: 673.0; found: 673.0.

i89) Ethyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0732] 



[0733] 1,2,4,6-Tetra-O-acetyl-3-azido-3-deoxy-β-D-galactopyranose (208 mg) was dissolved in CH2Cl2 and molecular sieves (MS3Å) was added. The mixture was cooled to 0 °C under N2 and EtSH (0.080 mL) was added followed by BF3•OEt2 (0.070 mL). After 2 h the reaction mixture was allowed to reach r.t. After four days NEt3 was added and the mixture was filtered to remove MS3Å and concentrated in vacuo. The residue was purified by flash chromatography (SiO2, 1-50% EtOAc in petroleum ether) to give 97 mg ethyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-β-D-galactopyranoside. This was redissolved in CH2Cl2 (3 mL) and BF3•OEt2 (0.070 mL) was added. After four days NEt3 was added and the mixture was concentrated in vacuo. The residue was purified by flash chromatography (SiO2, 5-50% EtOAc in petroleum ether) to give 43 mg ethyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside. 1H NMR (400 MHz, Chloroform-d) δ 5.74 (d, J = 5.5 Hz, 1H), 5.42 (s, 1H), 5.20 (dd, J = 10.8, 5.5 Hz, 1H), 4.53 (t, J = 6.3 Hz, 1H), 4.13 (dd, J = 11.4, 5.6 Hz, 1H), 4.04 (dd, J = 11.3, 7.2 Hz, 1H), 3.96 - 3.86 (m, 1H), 2.66 - 2.47 (m, 2H), 2.15 (s, 3H), 2.14 (s, 3H), 2.05 (s, 3H), 1.28 (t, J = 7.4 Hz, 3H).

i90) (5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide , isomer 1 and


i91) (5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide , isomer 2



[0734] 



[0735] mCPBA (242.91 mg, 1.20 mmol) was added it to a solution of 5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)- 1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (526 mg, 0.80 mmol) in CH2Cl2 (15 mL) at 0 °C. After stirring at 0 °C for 2 h, 10% aq NaOH was added it to mixture. Then it was extracted with CH2Cl2 (15 mL X 3). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product which was separated by preparative TLC (EtOAc/petroleum ether/CH2Cl2) to give isomer 1 (145 mg) and isomer 2 (40 mg). The exact chirality of the stereocenter located at the sulfurus atom marked * was not determined for isomer 1 and isomer 2.

i90) (5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide , isomer 1



[0736] 1H NMR (400 MHz, MeOD) δ 8.84 (d, J = 1.8 Hz, 1H), 8.82 (d, J = 2.0 Hz, 1H), 8.49 (s, 1H), 8.40 (t, J = 2.0 Hz, 1H), 7.58 - 7.49 (m, 2H), 6.24 (dd, J = 11.6, 5.6 Hz, 1H), 5.97 (dd, J = 11.6, 3.0 Hz, 1H), 5.29 (d, J = 5.6 Hz, 1H), 4.57 (t, J = 6.4 Hz, 1H), 3.89 (ddd, J = 18.8, 11.8, 6.1 Hz, 2H), 1.93 (s, 3H), 1.90 (s, 3H), 1.66 (s, 3H).
ESI-MS m/z calcd for [C25H22BrF3N4O8S]+ [M+H] +: 674.0; found: 675.1.

i91) (5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide, isomer 2



[0737] 1H NMR (400 MHz, MeOD) δ 8.82 (d, J = 1.8 Hz, 1H), 8.81 (d, J = 2.1 Hz, 1H), 8.50 (s, 1H), 8.34 (t, J = 2.0 Hz, 1H), 7.63 - 7.43 (m, 1H), 6.46 (dd, J = 11.7, 6.6 Hz, 1H), 6.12 (dd, J = 11.7, 2.9 Hz, 1H), 5.63 (d, J = 1.8 Hz, 1H), 5.37 - 5.33 (m, 1H), 5.19 (d, J = 6.6 Hz, 1H), 3.81 (ddd, J = 19.4, 12.0, 6.4 Hz, 2H), 1.96 (s, 3H), 1.93 (s, 3H), 1.89 (s, 3H).
ESI-MS m/z calcd for [C25H22BrF3N4O8S]+ [M+H]+: 674.0; found: 675.1.

i93) 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide, isomer 1 and


i94) 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide, isomer 2



[0738] 



[0739] To a solution of 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (400 mg, 0.65 mmol) in DCM (20 mL) were added mCPBA (171 mg, 0.98 mmol). The reaction was stirred at room temperature for 4 h. The mixture was concentrated and the crude product was purified by flash chromatography(EtOAc:PE =1:10 to 1:2, Silica 40 g, 40 ml/min, normal phase sillica, uv254) to obtain the title compound as isomer 1(18 mg) and isomer 2(100 mg).

i93) 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide, isomer 1



[0740] 1H NMR (400 MHz, CDCl3) δ 8.75 (dd, J = 23.8, 2.0 Hz, 2H), 8.04 (t, J = 2.1 Hz, 1H), 7.87 (s, 1H), 7.54 - 7.42 (m, 2H), 6.49 (dd, J = 11.5, 6.6 Hz, 1H), 6.06 (dd, J = 11.6, 2.9 Hz, 1H), 5.62 (d, J = 1.5 Hz, 1H), 5.53 (t, J = 6.2 Hz, 1H), 5.03 (d, J = 6.6 Hz, 1H), 3.91 (dd, J = 6.1, 3.1 Hz, 2H), 2.11 (s, 3H), 2.05 (d, J = 5.7 Hz, 6H).
m/z calcd for [C25H22ClF3N4O8S]+ [M+H]+: 631.0; found: 631.0.

i94) 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-α-D-galactopyranoside sulfoxide, isomer 2



[0741] 1H NMR (400 MHz, CDCl3) δ 8.70 (dd, J = 6.9, 2.0 Hz, 2H), 8.10 (t, J = 2.1 Hz, 1H), 7.82 (s, 1H), 7.43 (dd, J = 8.1, 6.5 Hz, 2H), 6.35 (dd, J = 11.5, 5.6 Hz, 1H), 6.05 (dd, J = 11.5, 3.1 Hz, 1H), 5.70 (d, J = 1.9 Hz, 1H), 5.23 (d, J = 5.6 Hz, 1H), 4.87 (t, J = 6.2 Hz, 1H), 4.12 (dd, J = 12.7, 6.8 Hz, 2H), 2.10 (s, 3H), 2.04 (s, 3H), 1.61 (s, 3H).
m/z calcd for [C25H22ClF3N4O8S]+ [M+H]+: 631.0; found: 631.0.

i95) (N,N-Dimethylnaphtalen-5-amine N-Oxide)-2-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosyl sulfone



[0742] 



[0743] To a solution of 5-Dimethylamino-naphatlen-2-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (200 mg, 0.30 mmol) in DCM (10 mL) was added mCPBA (256.5 mg, 1.49 mmol). The reaction mixture was stirred at room temperature for 1 h, followed by addition of DCM (20 ml). The reaction mixture was washed with sat. Na2S2O3 aq.(230 ml), sat. aq. NaHCO3 (230 mL). The organic layer was dried over anhydrous sodium sulphate. Removal of solvent gave crude N-oxide (200 mg). m/z calcd for [C32H31F3N4O10S]+ [M+H]+: 721.0; found: 721.0. The material was dissolved in methanol (3 mL) was added Pd/C (5 mg). The reaction was stirred at at room temperature for 4h under a hydrogen atmosphere. The mixture was filtered and the filtrate was evaporated to dryness. The residue was purified by preparative-TLC to obtain the desired product (10 mg) m/z calcd for [C32H31F3N4O9S]+ [M+H]+: 705.0; found: 705.0.

i96) 3,4-dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-(3,4,5-trifluorobenzamido)-1-thio-α-D-galactopyranoside


3,4-dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0744] 



[0745] To a stirred solution of 3,4-dichlorobenzenethiol (0.95 g, 5.32 mmol) in dry DMF (4 ml) was added NaH (0.12 g, 5.32 mmol) at 0 °C. The mixture was stirred at rt for 30 minutes followed by addition of 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (0.93 g, 2.66 mmol). The reaction mixture was stirred at 50 °C overnight. Solvents were removed in vacuo and the resulting crude was diluted with CH2Cl2 and washed with water and brine. The organic layer was dried over Na2SO4, filtered and evaporated. The crude product was purified by silica gel column to give the tite compound as a white solid (300 mg).
ESI-MS m/z calcd for [C18H20Cl2N3O7S]+ (M+H)+: 492.0; found: 492.0.

2,4,6-Tri-O-acetyl-3-amino-3-deoxy-1-thio-α-D-galactopyranoside



[0746] 



[0747] 3,4-dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (300 mg, 0.61 mmol) was dissolved in MeOH (15 mL) and DCM (5 mL). Pd/C (10%Pd, 650 mg). The mixture was stirred at rt under H2 pressure for 16 hours. TLC indicated complete reaction. Pd/C was filtered and the filter was concentracted. The crude residue (220 mg) was used for next step.
ESI-MS m/z calcd for [C18H22Cl2NO7S]+ (M+H)+: 466.0; found: 466.0

3,4-dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-(3,4,5-trifluorobenzamido)-1-thio-α-D-galactopyranoside



[0748] 



[0749] 2,4,6-Tri-O-acetyl-3-deoxy-3-amino-1-thio-α-D-galactopyranoside (115 mg, 0.25 mol), HATU (187 mg, 0.49 mmol) and 3,4,5-trifluorobenzoic acid (52 mg, 0.30 mmol) were dissloved in DMF (2 ml). N,N-Diisopropylethylamine (159 mg, 1.23 mmol) was added. The mixture was stirred at rt for 3 hours. Then water (50 mL) was added. The mixture was extracted by EtOAc (120 ml). The EtOAc phase was washed by brine (100 mL), dried over Na2SO4, filtered and concentrated. The residue was purified by silca gel column (PE:EtOAc=1:2) to obtain the title compound (50 mg, yield 33%).
ESI-MS m/z calcd for [C25H23Cl2F3NO8S]+ (M+H)+: 624.0; found: 624.1.

i98) 3,4-Dichlorophenyl 3-deoxy-3-[4-carboxy)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(ethoxycarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0750] 



[0751] 3,4-dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (1.3 g, 2.64 mmol), Ethyl propiolate (777 mg, 7.92 mmol), Copper(I)Iodide (151 mg, 0.79 mmol) and Triethylamine (1336 mg, 13.2 mmol) was dissolved in DMF (15 mL). The mixture was stirred at 100°C for 1 hour. EtOAc (200 ml) was added. The mixture was filetred, washed by water (100 mL), dried by Na2SO4, filtered and concentrated. The residue was purified by flash chromatography on silica to give 860 mg (55%) of the title compound.
ESI-MS m/z calcd for [C23H26Cl2N3O9S]+ (M+H)+: 590.1; found: 590.1.

3,4-Dichlorophenyl 3-deoxy-3-[4-carboxy)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0752] 



[0753] 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(ethoxycarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (400 mg, 0.68 mmol) was dissolved in MeOH (10 mL). KOH (152.04 mg, 2.71 mmol) in water (3 ml) was added. The mixture was stirred at rt for 6 hours. Then the mixture was acidified using 2M HCl to PH=6. The mixture was purified by reverse-phase column chromatography to give 260 mg (88%) of the title compound.
ESI-MS m/z calcd for [C15H16Cl2N3O6S]+ (M+H)+: 436.0; found: 436.0.

i100) 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0754] 



[0755] To a solution of 5-Chloropyridin-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (310 mg, 0.68 mmol) in MeCN (15 mL) was added [(3,4-difluorophenyl)-ethynyl]-trimethylsilane (170.5 mg, 0.81 mmol), Copper iodide (38.6 mg, 0.20 mmol), CsF (153.93 mg, 1.01 mmol) and N,N-diethylethanamine (436.6 mg, 3.38 mmol). The reaction vessel was purged twice with nitrogen. The reaction mixture was stirred at room temperature overnight. The mixture was filtered and washed with EtOAc (50 mL), The filtrate was concentrated in vacuo to afford crude product, which was purified by FLASH CHROMATOGRAPHY to afford the title compound (120 mg, 30% yield).
ESI-MS m/z calcd for [C25H23ClF2N4O7S]+ [M+H]+: 596.1; found: 597.1.

i101) 5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0756] 



[0757] To a solution of 5-Bromopyridin-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (280 mg, 0.56 mmol) in MeCN (15 mL) was added [(3,4-difluorophenyl)-ethynyl]-trimethylsilane (140.39 mg, 0.67 mmol), Copper iodide (31.78 mg, 0.17 mmol), CsF (126.76 mg, 0.83 mmol) and N,N-diethylethanamine (215.69 mg, 1.67 mmol). The reaction vessel was purged twice with nitrogen. Then the mixture was stirred at room temperature overnight. The mixture was filtered and washed with EtOAc (50 mL), The filtrate was concentrated in vacuo to afford crude product, which was purified by FLASH CHROMATOGRAPHY to give the title compound(100 mg, 28.02% yield).
ESI-MS m/z calcd for [C25H23BrF2N4O7S]+ [M+H]+: 640.0; found: 640.1.

i102) 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(propyl-carbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(1-hydroxybutyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0758] 



[0759] 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (300 mg, 0.61 mmol), hex-1-yne-3-ol (120 mg, 1.22 mmol), Copper(I)Iodide (34.81 mg, 0.18 mmo) and Triethylamine (308mg,3.05 mmol) were added to a flask. DMF (3 mL) was added. The mixture was stirred at 100 °C for 1 hour under a nitrogen atmosphere. The mixture was filtered and concentracted in vacuum. Water (50 mL) and EtOAc (120 ml) was added and the phases were separated. The organic phase was washed by brine (100 mL), dried over Na2SO4, filtered and concentrated. The residue was purified by silica gel column to give the title compound.
ESI-MS m/z calcd for [C24H30Cl2N3O8S]+ (M+H)+: 590.1; found: 590.2.

2,4,6-tri-O-acetyl-3-deoxy-3-[4-(propyl-carbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0760] 



[0761] Dess-Martin periodinane (387 mg, 0.91 mmol, 1.2 eq.) was added to a solution of 3,4-Dichlorophenyl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(1-hydroxybutyl-carbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (450 mg, 0.76 mmol, 1.0 eq.) in DCM (5 ml) and the mixture was stirred overnight at r.t. Diethyl ether (50 ml) was added and the organic layer was washed with aq. Na2S2O3 (2 M, 50 ml), aq. K2CO3 (2 M, 50 ml), and water (50ml). The combined organic layers were dried (Na2SO4), concentrated in vacuo and dried in vacuum to give the crude target product (357 mg).
ESI-MS m/z calcd for [C24H28Cl2N3O8S]+ (M+H)+: 588.1; found: 588.1.

i103) 5-Chloro-6-trifluoromethyl-pyridine-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside


3,5-dichloro-2-iodopyridine



[0762] 



[0763] A mixture of 2-bromo-3,5-dichloropyridine (5.672 g, 25 mmol), sodium iodide (11241.7 mg, 75 mmol) and chlorotrimethylsilane (2716 mg, 25 mmol) in MeCN (50 mL) was heated under reflux for 45 min. The reaction mixture was then poured into a 2.0 M aqueous solution of sodium hydroxide (10 mL) and extracted with diethyl ether (20 mL x 3). The combined organic layers were washed with brine and evaporated to afford crude product, which was purified by biotage (EtOAc/PE=1% ∼ 10%, ISCO 40 g, 25 mL/min, normal phase silica gel, uv 254) to afford the target compound 3,5-dichloro-2-iodopyridine (3800 mg, 55.5 % yield) as a white solid.
1H NMR (400 MHz, MeOD) δ 8.35 (t, J = 4.5 Hz, 1H), 8.01 (d, J = 2.3 Hz, 1H). GC-MS m/z calcd for [C5H2Cl2IN]: 272.9; found: 273.0.

3,5-dichloro-2-(trifluoromethyl)pyridine



[0764] 



[0765] KF (87.18mg, 1.5 mmol) and CuI (285.80 mg, 1.5 mmol) were thoroughly mixed before being heated under vacuum (1 mm Hg) with the flame of a Bunsen burner with gentle shaking until an homogeneous greenish color was obtained. NMP (25 mL), trimethyl(trifluoromethyl)silane (213.88 mg, 1.5 mmol). The mixture was stirred at 50 °C for 45 min. 3,5-dichloro-2-iodopyridine (2250 mg, 7.45 mmol) was added. The mixture was stirred at 50°C overnight. The reaction was followed by GC-MS, which indicated formed product. Water (50 mL) was added to mixture and extracted with Ethyl acetate (5 mL x 3). The combined organic layers were washed with brine and evaporated to afford crude product, which was purified by biotage (EtOAc/PE=1% ∼ 50%, ISCO 12 g, 10 mL/min, normal phase silica gel,uv 254) to afford compound 3,5-dichloro-2-(trifluoromethyl)pyridine (198 mg, 91.63% yield) as brown oil.
1H NMR (400 MHz, MeOD) δ 8.66 (d, J = 2.0 Hz, 1H), 8.34 - 8.24 (m, 1H). GC-MS m/z calcd for [C6H2Cl2F3N]: 215.0; found: 215.0.

2.3 5-chloro-6-(trifluoromethyl)pyridine-3-thiol



[0766] 



[0767] 3,5-dichloro-2-(trifluoromethyl)pyridine (1080 mg, 5.0 mmol) and NaHS (336.4 mg, 6.0 mmol) were dissolved in DMF (15 mL). The reaction mixture was stirred at room temperature for 3 h followed by adjustment to pH ∼ 9 by addition of 10% aq NaOH. The reaction mixture was extracted with Et2O (10 mL x 3) and the aqueous layer was acidified with 2 M NaHSO4 to PH ∼ 3. The mixture was extracted with ethyl acetate (15 mL x 3). The combined organic layers were washed with brine and evaporated to afford crude product, which was purified by biotage (EtOAc/PE=1% ∼ 50%, ISCO 20 g, 15 mL/min, normal phase silica gel, uv 254) to afford 5-chloro-6-(trifluoromethyl)pyridine-3-thiol (650 mg, 61% yield) as brown oil, which was used for next without further purification.
1H NMR (400 MHz, MeOD) δ 8.48 (d, J = 1.4 Hz, 1H), 8.02 (d, J = 17.1 Hz, 2H). ESI-MS m/z calcd for [C6H3ClF3NS]+ (M-H)-: 213.0; found: 211.9.

5-Chloro-6-trifluoromethyl-pyridine-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0768] 



[0769] NaH (20.5 mg, 0.86 mmol) was added to a solution of 5-chloro-6-(trifluoromethyl)pyridine-3-thiol (183.2 mg, 0.86 mmol) in DMF (10 mL) at 0 °C. The solution was stirred at room temperature for 30 min. Then 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (250 mg, 0.71 mmol) was added to mixture. The reaction was stirred at 50 °C for 2 h. The mixture was cooled to room temperature and water (50 mL) was added. Then it was extracted with EtOAc (10 mL X 3). The combined organic layers were washed with brine, dried over Na2SO4 and concentrated in vacuo to afford crude product, which was purified by biotage (EtOAc/PE=5% ∼ 40%, ISCO 12 g, 10 mL/min, normal phase silica gel,uv 254) to afford the target compound 3-azido-1,3-dideoxy-2,4,6-tri-O-acetyl-1-(5-chloro-6-(trifluoromethyl)pyridin-3-ylthio)-α-D-galactopyranoside (110 mg, 29% yield). ESI-MS m/z calcd for [C18H18ClF3N4O7S]+ [M+H]+: 526.1; found: 527.2

i105) 5-Chloro-6-cyano-pyridine-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


3-chloro-5-mercaptopicolinonitrile



[0770] 



[0771] To a solution of 3,5-dichloropicolinonitrile (300 mg, 1.74 mmol) in DMF (8 mL) was added Na2S•9H2O (487 mg, 1.74 mmol). The reaction was stirred at room temperature for 20 h. The pH was adjusted to pH 4-5 using NaHSO4 aq followed by addition of MTBE (15 mL). THe phases were separated and the organic phase was washed with water (20 mL) and brine (20 mL), dried over anhydrous sodium sulphate and the solvents were evaporated to give the desired product.
m/z calcd for [C6H3ClN2S]- [M-H]-: 169.0; found: 169.0.

5-Chloro-6-cyano-pyridine-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0772] 



[0773] To a solution of 3-chloro-5-mercaptopicolinonitrile (250 mg, 1.47 mmol) in DMF (4 mL) was added NaH (64 mg, 1.62 mmol) at 0°C . After 10 min, 5-Chloro-6-cyanopyridine-3-yl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (256 mg, 0.73 mmol) was added. The reaction mixture was stirred at room temperature for 4 h. Water (20 mL) and DCM (10 mL) were added. The aqueous phase was extracted with DCM (10 mL X 2) and the combined organic phases were washed with water (20 mL) and brine (20 mL), dried over anhydrous sodium sulphate. The solvents were evaporated followed by purification by column chromatography (PE/EtOAc=3/1) to obtain the desired product (80 mg, 23%).
m/z calcd for [C18H18ClN5O7S]+ [M+H]+: 484.0; found: 484.0.

5-Chloro-6-cyano-pyridine-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0774] 



[0775] To a solution of 5-Chloro-6-cyano-pyridine-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside (100 mg, 0.21 mmol) in DMF (5 mL) were added TEA (104.6 mg, 1.03 mmol), Copper(I)Iodide(11.8 mg, 0.06 mmol), CsF(47.1 mg, 0.31 mmol), [(3,4-difluorophenyl)-ethynyl]-trimethylsilane (65.2 mg, 0.31 mmol). The reaction was stirred at room temperature for 2 h under N2. Water (20 mL) and DCM (20 mL) were added. The aqueous phase was extracted with DCM (10 mL X 2), the combined organic phases were washed with water (20 mL) and brine (20 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The residue was purified by column chromatography(PE/EtOAc=3/1) to obtain the desired product (60 mg, 46.68%).
m/z calcd for [C26H22ClF2N5O7S]+ [M+H]+: 622.0; found: 622.0.

i106) 5-Chloro-6-cyano-pyridine-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside


2-chloro-4-mercaptobenzonitrile



[0776] 



[0777] To a solution of 2-chloro-4-fluoro-benzonitrile (4 g, 25.71 mmol) in DMF (20 mL) were added Na2S (3.01 g, 38.57 mmol). The mixture was held at room temperature with stirring on for 20 h. Then the mixture was added 1 M HCl to adjust to pH 6-7. MTBE (20 ml) and water (20 ml) was added. The aqueous phase was extracted with MTBE (20 mL X 2), the combined organic phases were washed with sat. NaHCO3 (50 mL) and brine (50 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a yellow solid (3.7 g). The residue was used to next step directly.
m/z calcd for [C7H4ClNS]- [M-H]-: 168.0; found: 168.0.

3-Chloro-4-cyanophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside



[0778] 



[0779] To a solution of 2-chloro-4-sulfanyl-benzonitrile (3.4 g, 20 mmol) in DMF (40 mL) was added NaH (529 mg, 22 mmol) at 0°C. After 10 min, 2,4,6-tri-O-acetyl-3-azido-1-chloro-3-deoxy-β-D-galactopyranoside (3.5 g, 10 mmol) was added. The mixture was stirred at room temperature for 4 h. Water (80 mL) and DCM (80 mL) were added. The aqueous phase was extracted with DCM (40 mL X 2), the combined organic phases were washed with water (100 mL) and brine (100 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The residue was purified by column chromatography (PE/EtOAc=3/1) to obtain the desired product (2 g, 21%).
m/z calcd for [C19H19ClN4O7S]+ [M+H]+: 483.0; found: 483.0.

5-Chloro-6-cyano-pyridine-3-yl 2,4,6-tri-O-acetyl-3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside



[0780] 



[0781] To a solution of 3-Chloro-4-cyanophenyl 2,4,6-tri-O-acetyl-3-azido-3-deoxy-1-thio-α-D-galactopyranoside (200 mg, 0.41 mmol) in DMF (5 mL) were added DIPEA (267.6, 2.07 mmol), Copper(I)Iodide(23.6 mg, 0.12 mmol), CsF(94.4mg, 0.62 mmol), [(3,4-difluorophenyl)-ethynyl]-trimethylsilane (130.6 mg, 0.62 mmol). The reaction was stirred at room temperature for 2 h under N2. Water (20 mL) and DCM (20 mL) were added. The aqueous phase was extracted with DCM (10 mL X 2), the combined organic phases were washed with water (50 mL) and brine (50 mL), dried over anhydrous sodium sulphate. Removal of solvent gave a residue. The residue was purified by column chromatography (PE/EtOAc=2/1) to obtain the desired product. (180 mg, 69.9%).
m/z calcd for [C27H23ClF2N4O7S]+ [M+H]+: 621.0; found: 621.0.
1H NMR (400 MHz, CDCl3) δ 7.77 (s, 1H), 7.68 - 7.59 (m, 2H), 7.54 - 7.48 (m, 1H), 7.46 (dd, J = 8.2, 1.7 Hz, 1H), 7.22 (dd, J = 13.3, 5.0 Hz, 1H), 6.33 (d, J = 5.6 Hz, 1H), 6.13 (dd, J = 11.7, 5.6 Hz, 1H), 5.62 (d, J = 2.3 Hz, 1H), 5.20 (dd, J = 11.7, 3.1 Hz, 1H), 4.78 - 4.73 (m, 1H), 4.21 - 4.03 (m, 2H), 2.08 (s, 3H), 1.96 (d, J = 1.3 Hz, 6H).

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Claims

1. A D-galactopyranose compound of formula (1)

wherein
the pyranose ring is α-D-galactopyranose,
A is selected from

wherein Het1 is selected from a five or six membered heteroaromatic ring, optionally substituted with a group selected from Br; F; Cl; CN; NR19R20, wherein R19 and R20 are independently selected from H, C1-3 alkyl, cyclopropyl, iso-propyl, - C(=O)-R21, wherein R21 is selected from H and C1-3 alkyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; iso-propyl, optionally substituted with a F; O-cyclopropyl optionally substituted with a F; O-isopropyl optionally substituted with a F; and OC1-3 alkyl optionally substituted with a F;

wherein R1-R5 are independently selected from H, CN, NH2, F, methyl optionally substituted with a F, and OCH3 optionally substituted with a F;

wherein R6 is selected from C1-6 alkyl optionally substituted with a halogen, branched C3-6 alkyl and C3-7 cycloalkyl;

X is selected from S, SO, SO2, O, C=O, and CR7R8 wherein R7 and R8 are independently selected from hydrogen, OH, or halogen;

wherein R27 is selected from a C1-6 alkyl, branched C3-6 alkyl, C1-6 alkoxy and branched C3-6 alkoxy;

B is selected from a) a C1-6 alkyl or branched C3-6 alkyl substituted with a five or six membered heteroaromatic ring, optionally substituted with a substituent selected from CN, a halogen, methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R14-CONH- wherein R14 is selected from C1-3 alkyl and cyclopropyl; or a C1-6 alkyl substituted with a phenyl, optionally substituted with a substituent selected from CN, a halogen, methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R15-CONH- wherein R15 is selected from C1-3 alkyl and cyclopropyl; b) an aryl, such as phenyl or naphthyl, optionally substituted with a group selected from a halogen; CN; -COOH;-CONR22R23, wherein R22 and R23 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; NR28R29, wherein R28 and R29 are independently selected from H, C1-3 alkyl and isopropyl; OH; and R16-CONH- wherein R16 is selected from C1-3 alkyl and cyclopropyl; c) a C5-7 cycloalkyl, optionally substituted with a substituent selected from a halogen, CN, methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R17-CONH- wherein R17 is selected from C1-3 alkyl and cyclopropyl; and d) a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with a group selected from a halogen; CN; -COOH;-CONR24R25, wherein R24 and R25 are independently selected from H, C1-3 alkyl, cyclopropyl, and iso-propyl; C1-3 alkyl, optionally substituted with a F; cyclopropyl, optionally substituted with a F; isopropyl, optionally substituted with a F; OC1-3 alkyl, optionally substituted with a F; O-cyclopropyl, optionally substituted with a F; O-isopropyl, optionally substituted with a F; NR30R31, wherein R30 and R31 are independently selected from H, C1-3 alkyl and isopropyl; OH; and R18-CONH- wherein R18 is selected from C1-3 alkyl and cyclopropyl; e) a C1-6 alkyl or branched C3-6 alkyl; or

a pharmaceutically acceptable salt or solvate thereof.
 
2. The compound of claim 1 wherein A is selected from formula 2 wherein R1-R5 are independently selected from H, F, methyl optionally substituted with a F, and OCH3 optionally substituted with a F.
 
3. The compound of claim 1 wherein A is selected from formula 3 wherein Het1 is selected from a six membered heteroaromatic ring, optionally substituted with a group selected from Br, F, and Cl.
 
4. The compound of claim 1 wherein A is selected from formula 4 wherein R6 is selected from C1-6 alkyl optionally substituted with a halogen, and branched C3-6 alkyl, such as CH3, CH2CH3, CH2CH2CH3, isopropyl and CH2CF3.
 
5. The compound of any one of claims 1-4 wherein A is selected from formula 8 wherein R27 is selected from a C1-6 alkyl, branched C3-6 alkyl, C1-6 alkoxy and branched C3-6 alkoxy.
 
6. The compound of any one of claims 1-5 wherein X is selected from S, SO, SO2, and O, such as S, SO, and SO2.
 
7. The compound of any one of claims 1-6 wherein B is selected from a C1-6 alkyl substituted with a phenyl, said phenyl optionally substituted with a group selected from Br, F, Cl, methyl optionally substituted with a F, and OCH3 optionally substituted with a F.
 
8. The compound of any one of claims 1-6 wherein B is selected from a C1-6 alkyl, such as C1-4 alkyl, e.g. ethyl, propyl and butyl.
 
9. The compound of any one of claims 1-6 wherein B is selected from an aryl, such as phenyl or naphthyl, optionally substituted with a group selected from a halogen; CN; methyl optionally substituted with a F; OCH3 optionally substituted with a F; OCH2CH3 optionally substituted with a F; OH; R16-CONH- wherein R16 is selected from C1-3 alkyl and cyclopropyl; -COOH; NR28R29, wherein R28 and R29 are independently selected from H, C1-3 alkyl and isopropyl; and -CONH2.
 
10. The compound of any one of claims 1-6 wherein B is selected from a heterocycle, such as heteroaryl or heterocycloalkyl, optionally substituted with a group selected from a halogen; CN; methyl optionally substituted with a F; OCH3 optionally substituted with a F; OCH2CH3 optionally substituted with a F; OH; CONH2; NR30R31, wherein R30 and R31 are independently selected from H, C1-3 alkyl and isopropyl; and R18-CONH- wherein R18 is selected from C1-3 alkyl and cyclopropyl.
 
11. The compound of any one of claims 1-6 wherein B is selected from a C5-7 cycloalkyl, optionally substituted with a substituent selected from a halogen, methyl optionally substituted with a F, OCH3 optionally substituted with a F, OCH2CH3 optionally substituted with a F, OH, and R17-CONH- wherein R17 is selected from C1-3 alkyl and cyclopropyl.
 
12. The compound of any one of claims 1-11 selected from:

3,4-Dimethylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Ethoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-a-D-galactopyranoside,

4-Pyridyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Acetanilidyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Methoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranoside,

2,3-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranoside,

Benzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Methoxyphenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Naphtyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Methylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-(Trifluoromethyl)phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,5-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranoside,

2,6-Dimethylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

1-Naphthyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-(Trifluoromethoxy)phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Pyridyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl-1H-1,2,3-triazol-1-yl]-)-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-2-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

Phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Tolyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Trifluoromethoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

Cyclohexyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2,4,5-Trichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2,5-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Hydroxy-phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

2-Phenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-5-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-6-fluoro-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromo-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Bromo-6-trifluoromethyl-pyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-cyano-pyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-cyano-pyridazin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

5-Methoxy-6-cyano-pyridazin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Hydroxy-6-cyano-pyridazin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-2,4-difluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(phenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,5-Dichloro-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichloro-6-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromo-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-4-(trifluoromethyl)phenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3,4,5-Trichlorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

5-Chloro-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

5-Bromo-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

5-Chloro-2-methoxyphenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3-Iodophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

Picolinamide-5-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Cyanophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

2-Cyanopyridine-5-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Chloro-2-thienyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Carboxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Benzamide-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3-fluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,3'-difluoro-cyklohexyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

n-Butyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,5-difluoro-4-methoxyphenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Hydroxy-pyridin-4-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Chlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3,4-Dichlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Chlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

Propyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Aminopyridin-4-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-dimethylamino-naphatlen-2-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Ethyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

S-5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

R-5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

S-5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

R-5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxide,

5-Dimethylamino-naphtalen-2-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

3,4-Dichlorophenyl 3-deoxy-3-[4-(ethoxycarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(ethylaminocarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophenyl 3-deoxy-3-[4-(propyl-carbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-trifluoromethyl-pyridine-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-2-trifluoromethyl-pyridine-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-cyano-pyridine-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, and

3-Chloro-4-cyanophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside.


 
13. A pharmaceutical composition comprising the compound of any one of the previous claims and optionally a pharmaceutically acceptable additive, such as carrier or excipient.
 
14. The compound of any one of the claims 1- 12, or a pharmaceutical composition according to claim 13 for use in a method for treating a disorder relating to the binding of a galectin-3 to a ligand in a mammal, wherein said disorder is selected from the group consisting of inflammation; fibrosis, such as pulmonary fibrosis, liver fibrosis, kidney fibrosis, ophthalmological fibrosis and fibrosis of the skin and heart; scarring; keloid formation; aberrant scar formation; surgical adhesions; septic shock; cancer, such as carcinomas, sarcomas, leukemias and lymphomas, such as T-cell lymphomas; metastasising cancers; autoimmune diseases, such as psoriasis, rheumatoid arthritis, Crohn's disease, ulcerative colitis, ankylosing spondylitis, systemic lupus erythematosus; metabolic disorders; heart disease; heart failure; pathological angiogenesis, such as ocular angiogenesis or a disease or condition associated with ocular angiogenesis, e.g. neovascularization related to cancer; and eye diseases, such as age-related macular degeneration and corneal neovascularization; atherosclerosis; metabolic diseases such as diabetes; asthma and other interstitial lung diseases, including Hermansky-Pudlak syndrome, mesothelioma; liver disorders, such as non-alcoholic steatohepatitis.
 


Ansprüche

1. Eine D-Galactopyranose-Verbindung der Formel (1)

wobei
der Pyranosering α-D-Galactopyranose ist,
A ausgewählt ist aus

wobei Het1 ausgewählt ist aus einem fünf- oder sechsgliedrigen heteroaromatischen Ring, wahlweise substituiert durch eine Gruppe ausgewählt aus Br; F; Cl; CN; NR19R20, wobei R19 und R20 unabhängig voneinander aus H, (C1-3)-Alkyl, Cyclopropyl, Isopropyl, -C(=O)-R21 ausgewählt sind, wobei R21 ausgewählt ist aus H und (C1-3)-Alkyl; (C1-3)-Alkyl, wahlweise substituiert durch ein F; Cyclopropyl, wahlweise substituiert durch ein F; Isopropyl, wahlweise substituiert durch ein F; O-Cyclopropyl, wahlweise substituiert durch ein F; O-Isopropyl, wahlweise substituiert durch ein F; und O(C1-3)-Alkyl, wahlweise substituiert durch ein F;

wobei R1-R5 unabhängig voneinander aus H, CN, NH2, F, Methyl, wahlweise substituiert durch ein F, und OCH3, wahlweise substituiert durch ein F, ausgewählt sind;

wobei R6 ausgewählt ist aus (C1-6)-Alkyl, wahlweise substituiert durch ein Halogen, verzweigtem (C3-6)-Alkyl und (C3-7)-Cycloalkyl;

X ausgewählt aus S, SO, SO2, O, C=O, und CR7R8 ist, wobei R7 und R8 unabhängig voneinander aus Wasserstoff, OH, oder Halogen ausgewählt sind;

wobei R27 ausgewählt aus einem (C1-6)-Alkyl, verzweigtem (C3-6)-Alkyl, (C1-6)-Alkoxy und verzweigtem (C3-6)-Alkoxy ist;

B ausgewählt ist aus a) einem (C1-6)-Alkyl oder verzweigten (C3-6)-Alkyl, das durch einen fünf- oder sechsgliedrigen heteroaromatischen Ring substituiert ist, wahlweise substituiert durch einen Substituenten, der ausgewählt ist aus CN, einem Halogen, Methyl, wahlweise substituiert durch ein F, OCH3, wahlweise substituiert durch ein F, OCH2CH3, wahlweise substituiert durch ein F, OH, und R14-CONH-, wobei R14 ausgewählt aus (C1-3)-Alkyl und Cyclopropyl ist; oder einem (C1-6)-Alkyl, substituiert durch ein Phenyl, wahlweise substituiert durch einen Substituenten ausgewählt aus CN, einem Halogen, Methyl, wahlweise substituiert durch ein F, OCH3, wahlweise substituiert durch ein F, OCH2CH3, wahlweise substituiert durch ein F, OH, und R15-CONH-, wobei R15 ausgewählt aus (C1-3)-Alkyl und Cyclopropyl ist; b) einem Aryl, wie etwa Phenyl oder Naphthyl, wahlweise substituiert durch eine Gruppe ausgewählt aus einem Halogen; CN; -COOH; -CONR22R23, wobei R22 und R23 unabhängig voneinander aus H, (C1-3)-Alkyl, Cyclopropyl, und Isopropyl ausgewählt sind; (C1-3)-Alkyl, wahlweise substituiert durch ein F; Cyclopropyl, wahlweise substituiert durch ein F; Isopropyl, wahlweise substituiert durch ein F; O(C1-3)-Alkyl, wahlweise substituiert durch ein F; O-Cyclopropyl, wahlweise substituiert durch ein F; O-Isopropyl, wahlweise substituiert durch ein F; NR28R29, wobei R28 und R29 unabhängig voneinander aus H, (C1-3)-Alkyl und Isopropyl ausgewählt sind; OH; und R16-CONH-, wobei R16 ausgewählt aus (C1-3)-Alkyl und Cyclopropyl ist; c) einem)(C5-7)-Cycloalkyl, wahlweise substituiert durch einen Substituenten ausgewählt aus einem Halogen, CN, Methyl, wahlweise substituiert durch ein F, OCH3, wahlweise substituiert durch ein F, OCH2CH3, wahlweise substituiert durch ein F, OH, und R17-CONH-, wobei R17 ausgewählt aus (C1-3)-Alkyl und Cyclopropyl ist; und d) einem Heterocyclus, wie etwa Heteroaryl oder Heterocycloalkyl, wahlweise substituiert durch eine Gruppe ausgewählt aus einem Halogen; CN; -COOH; -CONR24R25, wobei R24 und R25 unabhängig voneinander ausgewählt sind aus H, (C1-3)-Alkyl, Cyclopropyl, und Isopropyl; (C1-3)-Alkyl, wahlweise substituiert durch ein F; Cyclopropyl, wahlweise substituiert durch ein F; Isopropyl, wahlweise substituiert durch ein F; O(C1-3)-Alkyl, wahlweise substituiert durch ein F; O-Cyclopropyl, wahlweise substituiert durch ein F; O-Isopropyl, wahlweise substituiert durch ein F; NR30R31, wobei R30 und R31 unabhängig voneinander ausgewählt sind aus H, (C1-3)-Alkyl und Isopropyl; OH; und R18-CONH-, wobei R18 ausgewählt ist aus (C1-3)-Alkyl und Cyclopropyl; e) einem (C1-6)-Alkyl oder verzweigtem (C3-6)-Alkyl; oder

ein pharmazeutisch akzeptables Salz oder Solvat davon.
 
2. Die Verbindung des Anspruchs 1, wobei A aus der Formel 2 ausgewählt ist, wobei R1-R5 unabhängig voneinander aus H, F, Methyl, wahlweise substituiert durch ein F, und OCH3, wahlweise substituiert durch ein F, ausgewählt sind.
 
3. Die Verbindung des Anspruchs 1, wobei A aus der Formel 3 ausgewählt ist, wobei Het1 ausgewählt ist aus einem sechsgliedrigen heteroaromatischen Ring, wahlweise substituiert durch eine Gruppe ausgewählt aus Br, F, und Cl.
 
4. Die Verbindung des Anspruchs , wobei A aus der Formel 4 ausgewählt ist, wobei R6 ausgewählt ist aus (C1-6)-Alkyl, wahlweise substituiert durch ein Halogen, und verzweigtem (C3-6)-Alkyl, wie etwa CH3, CH2CH3, CH2CH2CH3, Isopropyl und CH2CF3.
 
5. Die Verbindung von einem der Ansprüche 1-4, wobei A ausgewählt aus der Formel 8 ist, wobei R27 ausgewählt aus einem (C1-6)-Alkyl, verzweigten (C3-6)-Alkyl, (C1-6)-Alkoxy und verzweigten (C3-6)-Alkoxy ist.
 
6. Die Verbindung von einem der Ansprüche 1-5, wobei X ausgewählt aus S, SO, SO2, und O, wie etwa S, SO, und SO2 ist.
 
7. Die Verbindung von einem der Ansprüche 1-6, wobei B ausgewählt ist aus einem durch ein Phenyl substituierten (C1-6)-Alkyl, wobei das Phenyl wahlweise substituiert ist durch eine Gruppe ausgewählt aus Br, F, Cl, Methyl, wahlweise substituiert durch ein F, und OCH3, wahlweise substituiert durch ein F.
 
8. Die Verbindung von einem der Ansprüche 1-6, wobei B ausgewählt aus einem (C1-6)-Alkyl, wie etwa (C1-4)-Alkyl, z. B. Ethyl, Propyl und Butyl ist.
 
9. Die Verbindung von einem der Ansprüche 1-6, wobei B ausgewählt ist aus einem Aryl, wie etwa Phenyl oder Naphthyl, wahlweise substituiert durch eine Gruppe ausgewählt aus einem Halogen; CN; Methyl, wahlweise substituiert durch ein F; OCH3, wahlweise substituiert durch ein F; OCH2CH3, wahlweise substituiert durch ein F; OH; R16-CONH-, wobei R16 ausgewählt ist aus (C1-3)-Alkyl und Cyclopropyl;-COOH; NR28R29, wobei R28 und R29 unabhängig voneinander aus H, (C1-3)-Alkyl und Isopropyl; und -CONH2 ausgewählt sind.
 
10. Die Verbindung von einem der Ansprüche 1-6, wobei B ausgewählt ist aus einem Heterocyclus, wie etwa Heteroaryl oder Heterocycloalkyl, wahlweise substituiert durch eine Gruppe ausgewählt aus einem Halogen; CN; Methyl, wahlweise substituiert durch ein F; OCH3, wahlweise substituiert durch ein F; OCH2CH3, wahlweise substituiert durch ein F; OH; CONH2; NR30R31, wobei R30 und R31 unabhängig voneinander ausgewählt sind aus H, (C1-3)Alkyl und Isopropyl; und R18-CONH-, wobei R18 ausgewählt ist aus (C1-3)-Alkyl und Cyclopropyl.
 
11. Die Verbindung von einem der Ansprüche 1-6, wobei B ausgewählt ist aus einem (C5-7)-Cycloalkyl, wahlweise substituiert durch einen Substituenten ausgewählt aus einem Halogen; Methyl, wahlweise substituiert durch ein F; OCH3, wahlweise substituiert durch ein F; OCH2CH3, wahlweise substituiert durch ein F; OH und R17-CONH-, wobei R17 ausgewählt ist aus (C1-3)-Alkyl und Cyclopropyl.
 
12. Die Verbindung von einem der Ansprüche 1-11, die ausgewählt ist aus:

3,4-Dimethylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Ethoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

4-Pyridyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

2,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

4-Acetanilidyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

4-Methoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranosid,

2,3-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio -α-D-galactopyranosid,

Benzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Methoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

2-Naphtyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Methylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-(Trifluoromethyl)phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

4-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,5-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranosid,

2,6-Dimethylphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

1-Naphthyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-(Trifluoromethoxy)phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

2-Pyridyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl-1H-1,2,3-triazol-1-yl]-)-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfoxid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfon,

4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfoxid,

Phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Chloro-4-fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

4-Tolyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

4-Fluorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

4-Trifluoromethoxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

Phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

3-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

4-Chlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

Cyclohexyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

2,4,5-Trichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

2,5-Dichlorophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Hydroxy-phenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Bromophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfoxid,

2-Phenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfoxid,

5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Chloro-5-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Chloro-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Chloro-6-fluoro-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Bromo-4-cyanophenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Bromo-6-trifluoromethyl-pyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Chloro-6-cyano-pyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Chloro-6-cyano-pyridazin-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(2,3,4,5,6-pentafluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfon,

5-Methoxy-6-cyano-pyridazin-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Hydroxy-6-cyano-pyridazin-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Chloro-2,4-difluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy -3-[4-(phenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,5-Dichloro-4-fluorophenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichloro-6-fluorophenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Bromo-4-fluorophenyl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Chloro-4-(trifluoromethyl)phenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

3,4,5-Trichlorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-l-yl]-α-D-galactopyranosid,

5-Chloro-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

5-Bromo-2-fluorophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

5-Chloro-2-methoxyphenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

3-Iodophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

Picolinamid-5-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Cyanophenyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

2-Cyanopyridine-5-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

4-Chloro-2-thienyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3-Carboxyphenyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

Benzamid-3-yl 3-deoxy -3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3-fluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,3'-Difluoro-cyclohexyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

n-Butyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(3,5-difluoro-4-methoxyphenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

2-Hydroxy-pyridin-4-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

2-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

4-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

2-Chlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

3,4-Dichlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

3-Chlorophenetyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

4-Chlorobenzyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

Propyl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

2-Aminopyridin-4-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Dimethylamino-naphatlen-2-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

Ethyl 3-deoxy-1-thio-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosid,

S-5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfoxid,

R-5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfoxid,

5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfon,

S-5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfoxid,

R-5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfoxid,

5-Dimethylamino-naphtalen-2-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosylsulfon,

3,4-Dichlorophenyl 3-deoxy-3-[4-(ethoxycarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(ethylaminocarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Chloropyridin-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Bromopyridin-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

3,4-Dichlorophenyl 3-deoxy-3-[4-(propyl-carbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Chloro-6-trifluoromethyl-pyridine-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Chloro-2-trifluoromethyl-pyridine-3-yl 3-deoxy-3-[4-(3,4,5-trifluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid,

5-Chloro-6-cyano-pyridine-3-yl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid, und

3-Chloro-4-cyanophenyl 3-deoxy-3-[4-(3,4-difluorophenyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranosid.


 
13. Eine pharmazeutische Zusammensetzung umfassend die Verbindung von einem der vorhergehenden Ansprüche und wahlweise einen pharmazeutisch akzeptablen Zusatzstoff, wie etwa eine Trägersubstanz oder einen Hilfsstoff.
 
14. Die Verbindung von einem der Ansprüche 1-12, oder eine pharmazeutische Zusammensetzung nach Anspruch 13 zur Verwendung in einem Verfahren zur Behandlung von einer Erkrankung, die bezogen auf die Bindung von Galectin-3 an einen Liganden in einem Säugetier ist, wobei die Erkrankung ausgewählt ist aus der Gruppe bestehend aus Entzündung; Fibrose, wie etwa Lungenfibrose, Leberfibrose, Nierenfibrose, ophthalmologischer Fibrose und Fibrose der Haut und des Herzens; Narbenbildung; Keloidbildung; aberranter Narbenbildung; chirurgischen Adhäsionen; septischem Schock; Krebs, wie etwa Karzinomen, Sarkomen, Leukämien und Lymphomen, wie etwa T-Zell-Lymphomen; metastasierenden Krebsarten; Autoimmunerkrankungen, wie etwa Psoriasis, rheumatoider Arthritis, Morbus Crohn, Colitis ulcerosa, Spondylitis ankylosans, systemischem Lupus erythematodes; Stoffwechselstörungen; Herzkrankheit; Herzinsuffizienz; pathologischer Angiogenese, wie etwa Augenangiogenese oder einer Krankheit oder einem Zustand, die bzw. der mit Augenangiogenese verbunden ist, z. B. Neovaskularisation im Zusammenhang mit Krebs; und Augenkrankheiten, wie etwa altersbedingter Makuladegeneration und Hornhautneovaskularisation; Atherosklerose; Stoffwechselerkrankungen, wie etwa Diabetes; Asthma und anderen interstitiellen Lungenerkrankungen, einschließlich Hermansky-Pudlak-Syndrom, Mesotheliom; Lebererkrankungen, wie etwa nichtalkoholischer Steatohepatits.
 


Revendications

1. Un composé de type D-galactopyranose de formule (1)


l'anneau de pyranose est la α-D-galactopyranose,
A est choisi de

où Het1 est choisi parmi un anneau hétéroaromatique à cinq ou six chaînons, facultativement substitué par un groupe choisi parmi Br ; F ; Cl ; CN ; NR19R20, où R19 et R20 sont indépendamment choisis parmi H, alkyle en C1-3, cyclopropyle, iso-propyle, -C(=O)-R21, où R21 est choisi parmi H et alkyle en C1-3 ; alkyle en C1-3, facultativement substitué par un F ; cyclopropyle, facultativement substitué par un F ; iso-propyle, facultativement substitué par un F ; O-cyclopropyle facultativement substitué par un F ; O-isopropyle facultativement substitué par un F ; et alkyle en OC1-3 facultativement substitué par un F ;

où R1-R5 sont indépendamment choisis parmi H, CN, NH2, F, méthyle, facultativement substitué par un F, et OCH3, facultativement substitué par un F ;

où R6 est choisi parmi alkyle en C1-6, facultativement substitué par un halogène, alkyle en C3-6 branché et cycloalkyle en C3-7 ;

X est choisi parmi S, SO, SO2, O, C=O, et CR7R8, où R7 et R8 sont indépendamment choisis parmi hydrogène, OH, ou halogène ;

où R27 est choisi parmi un alkyle en C1-6, alkyle en C3-6 branché, alcoxyle en C1-6 et alcoxyle en C3-6 branché ;

B est choisi parmi a) un alkyle en C1-6 ou alkyle en C3-6 branché substitué par un anneau hétéroaromatique à cinq ou six chaînons, facultativement substitué par un substituant choisi parmi CN, un halogène, méthyle facultativement substitué par un F, OCH3 facultativement substitué par un F, OCH2CH3 facultativement substitué par un F, OH, et R14-CONH-, où R14 est choisi parmi alkyle en C1-3 et cyclopropyle ; ou un alkyle en C1-6 substitué par un phényle, facultativement substitué par un substituant choisi parmi CN, un halogène, méthyle facultativement substitué par un F, OCH3 facultativement substitué par un F, OCH2CH3 facultativement substitué par un F, OH, et R15-CONH-, où R15 est choisi parmi alkyle en C1-3 et cyclopropyle ; b) un aryle, tel que phényle ou naphtyle, facultativement substitué par un groupe choisi parmi un halogène ; CN ; -COOH ; -CONR22R23, où R22 et R23 sont indépendamment choisis parmi H, alkyle en C1-3, cyclopropyle, et iso-propyle ; alkyle en C1-3, facultativement substitué par un F ; cyclopropyle, facultativement substitué par un F ; isopropyle, facultativement substitué par un F ; alkyle en OC1-3, facultativement substitué par un F ; O-cyclopropyle, facultativement substitué par un F ; O-isopropyle, facultativement substitué par un F ; NR28R29, où R28 et R29 sont indépendamment choisis parmi H, alkyle en C1-3 et isopropyle ; OH ; et R16-CONH-, où R16 est choisi parmi alkyle en C1-3 et cyclopropyle ; c) un cycloalkyle en C5-7, facultativement substitué par un substituant choisi parmi un halogène, CN, méthyle facultativement substitué par un F, OCH3 facultativement substitué par un F, OCH2CH3 facultativement substitué par un F, OH, et R17-CONH-, où R17 est choisi parmi alkyle en C1-3 et cyclopropyle ; et d) un hétérocycle, tel qu'un hétéroaryle ou un hétérocycloalkyle, facultativement substitué par un groupe choisi parmi un halogène ; CN ; -COOH ; -CONR24R25, où R24 et R25 sont indépendamment choisis parmi H, alkyle en C1-3, cyclopropyle, et iso-propyle ; alkyle en C1-3, facultativement substitué par un F ; cyclopropyle, facultativement substitué par un F ; isopropyle, facultativement substitué par un F ; alkyle en OC1-3, facultativement substitué par un F ; O-cyclopropyle, facultativement substitué par un F ; O-isopropyle, facultativement substitué par un F ; NR30R31, où R30 et R31 sont indépendamment choisis parmi H, alkyle en C1-3 et isopropyle ; OH ; et R18-CONH-, où R18 est choisi parmi alkyle en C1-3 et cyclopropyle ; e) un alkyle en C1-6 ou un alkyle en C3-6 branché ; ou

un sel ou un solvat pharmaceutiquement acceptable de celui-ci.
 
2. Le composé de la revendication 1, dans lequel A est choisi de la formule 2, où R1-R5 sont indépendamment choisis parmi H, F, méthyle, facultativement substitué par un F, et OCH3, facultativement substitué par un F.
 
3. Le composé de la revendication 1, dans lequel A est choisi de la formule 3, où Het1 est choisi parmi un anneau hétéroaromatique à six chaînons, facultativement substitué par un groupe choisi parmi Br, F, et Cl.
 
4. Le composé de la revendication 1, dans lequel A est choisi de la formule 4, où R6 est choisi parmi alkyle en C1-6, facultativement substitué par un halogène, et alkyle en C3-6 branché, tel que CH3, CH2CH3, CH2CH2CH3, isopropyle et CH2CF3.
 
5. Le composé de l'une quelconque des revendications 1-4, dans lequel A est choisi de la formule 8, où R27 est choisi parmi un alkyle en C1-6, alkyle en C3-6 branché, alcoxyle en C1-6 et alcoxyle en C3-6 branché.
 
6. Le composé de l'une quelconque des revendications 1-5, dans lequel X est choisi parmi S, SO, SO2, et O, tel que S, SO, et SO2.
 
7. Le composé de l'une quelconque des revendications 1-6, dans lequel B est choisi parmi un alkyle en C1-6 substitué par un phényle, ledit phényle étant facultativement substitué par un groupe choisi parmi Br, F, Cl, méthyle facultativement substitué par un F, et OCH3 facultativement substitué par un F.
 
8. Le composé de l'une quelconque des revendications 1-6, dans lequel B est choisi parmi un alkyle en C1-6, tel qu'alkyle en C1-4, par. ex., éthyle, propyle et butyle.
 
9. Le composé de l'une quelconque des revendications 1-6, dans lequel B est choisi parmi un aryle, tel que phényle ou naphtyle, facultativement substitué par un groupe choisi parmi un halogène ; CN ; méthyle, facultativement substitué par un F ; OCH3, facultativement substitué par un F ; OCH2CH3, facultativement substitué par un F ; OH ; R16-CONH-, où R16 est choisi parmi alkyle en C1-3 et cyclopropyle ; -COOH ; NR28R29, où R28 et R29 sont indépendamment choisis parmi H, alkyle en C1-3 et isopropyle ; et -CONH2.
 
10. Le composé de l'une quelconque des revendications 1-6, dans lequel B est choisi parmi un hétérocycle, tel qu'hétéroaryle ou hétérocycloalkyle, facultativement substitué par un groupe choisi parmi un halogène ; CN ; méthyle, facultativement substitué par un F ; OCH3, facultativement substitué par un F ; OCH2CH3, facultativement substitué par un F ; OH ; CONH2 ; NR30R31, où R30 et R31 sont indépendamment choisis parmi H, alkyle en C1-3 et isopropyle ; et R18-CONH-, où R18 est choisi parmi alkyle en C1-3 et cyclopropyle.
 
11. Le composé de l'une quelconque des revendications 1-6, dans lequel B est choisi parmi un cycloalkyle en C5-7, facultativement substitué par un substituant choisi parmi un halogène, méthyle, facultativement substitué par un F ; OCH3, facultativement substitué par un F ; OCH2CH3, facultativement substitué par un F ; OH, et R17-CONH-, où R17 est choisi parmi alkyle en C1-3 et cyclopropyle.
 
12. Le composé de l'une quelconque des revendications 1-11 choisi parmi :

3,4-Diméthylphényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-thio-α-D-galactopyranoside,

3-Éthoxyphényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Pyridyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2,4-Dichlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Acétanilidyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Méthoxyphényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranoside,

2,3-Dichlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Benzyl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-thio-α-D-galactopyranoside,

3-Méthoxyphényl 3-désoxy -3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Naphtyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Méthylphényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-(Trifluorométhyl)phényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Bromophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-thio-α-D-galactopyranoside,

3,5-Dichlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio α-D-galactopyranoside,

2,6-Diméthylphényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

1-Naphthyl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-(Trifluorométhoxy)phényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Pyridyl 3-désoxy-3-[4-(3,4,5-trifluorophényl-1H-1,2,3-triazol-1-yl]-)-1-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

4-Chlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxyde,

3,4-Dichlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

4-Chlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxyde,

Phényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-4-fluorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Tolyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Fluorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Trifluorométhoxyphényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Phényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3-Chlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

4-Chlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

Cyclohexyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2,4,5-Trichlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2,5-Dichlorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Hydroxy-phényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxyde,

2-Phénetyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(2,3,4,5,6-pentafluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxyde,

5-Chloropyridin-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Bromopyridin-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-5-cyanophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-4-cyanophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-6-fluoro-4-cyanophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromo-4-cyanophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Bromo-6-trifluorométhyl-pyridin-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-cyano-pyridin-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-cyano-pyridazin-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(2,3,4,5,6-pentafluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(2,3,4,5,6-pentafluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

5-Méthoxy-6-cyano-pyridazin-3-yl 3-désoxy -3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Hydroxy-6-cyano-pyridazin-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-2,4-difluorophényl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(phényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,5-Dichloro-4-fluorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichloro-6-fluorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Bromo-4-fluorophényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Chloro-4-(trifluorométhyl)phényl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3,4,5-Trichlorophényl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

5-Chloro-2-fluorophényl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

5-Bromo-2-fluorophényl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

5-Chloro-2-méthoxyphényl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3-Iodophényl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

Picolinamide-5-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Cyanophényl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

2-Cyanopyridine-5-yl 3-désoxy -3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Chloro-2-thienyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3-Carboxyphényl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Benzamide-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(3,4-difluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(3,4-difluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(3-fluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,3'-difluoro-cyclohexyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

n-Butyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(3,5-difluoro-4-méthoxyphényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Hydroxy-pyridin-4-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Chlorophénetyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Chlorophénetyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

2-Chlorobenzyl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl D-galactopyranoside,

3,4-Dichlorobenzyl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

3-Chlorophénetyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

4-Chlorobenzyl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

Propyl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-thio-α-D-galactopyranoside,

2-Aminopyridin-4-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-diméthylamino-naphatlen-2-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

Éthyl 3-désoxy-1-thio-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranoside,

S-5-Bromopyridin-3-yl 3-désoxy-3-[4-(3,4,5-tiifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxyde,

R-5-Bromopyridin-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxyde,

5-Bromopyridin-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

S-5-Chloropyridin-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxyde,

R-5-Chloropyridin-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfoxyde,

5-Diméthylamino-naphtalen-2-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-α-D-galactopyranosyl sulfone,

3,4-Dichlorophényl 3-désoxy-3-[4-(éthoxycarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(éthylaminocarbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloropyridin-3-yl 3-désoxy-3-[4-(3,4-difluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Bromopyridin-3-yl 3-désoxy-3-[4-(3,4-difluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

3,4-Dichlorophényl 3-désoxy-3-[4-(propyl-carbonyl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-trifluorométhyl-pyridine-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-2-trifluorométhyl-pyridine-3-yl 3-désoxy-3-[4-(3,4,5-trifluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside,

5-Chloro-6-cyano-pyridine-3-yl 3-désoxy-3-[4-(3,4-difluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside, et

3-Chloro-4-cyanophényl 3-désoxy-3-[4-(3,4-difluorophényl)-1H-1,2,3-triazol-1-yl]-1-thio-α-D-galactopyranoside.


 
13. Une composition pharmaceutique comprenant le composé de l'une quelconque des revendications précédentes et facultativement un additif pharmaceutiquement acceptable, tel qu'un véhicule ou un excipient.
 
14. Le composé selon l'une quelconque des revendications 1-12, ou une composition pharmaceutique selon la revendication 13 pour l'utilisation dans un procédé de traitement d'un trouble lié à la liaison d'une galectine-3 à un ligand chez un mammifère, dans lequel ledit trouble est choisi dans le groupe constitué d'inflammation ; fibrose, telle que la fibrose pulmonaire, la fibrose hépatique, la fibrose rénale, la fibrose ophtalmologique et la fibrose de la peau et du cœur ; les cicatrices ; la formation chéloïde ; la formation de cicatrices aberrantes ; les adhérences chirurgicales ; le choc septique ; le cancer, tel que des carcinomes, des sarcomes, des leucémies et des lymphomes, tels que des lymphomes à cellules T ; des cancers métastasants ; des maladies auto-immunes, telles que le psoriasis, la polyarthrite rhumatoïde, la maladie de Crohn, la colite ulcéreuse, la spondylarthrite ankylosante, le lupus érythémateux disséminé ; des troubles métaboliques ; les cardiopathies ; l'insuffisance cardiaque ; l'angiogenèse pathologique, telle qu'une angiogenèse oculaire ou une maladie ou un état associé à une angiogenèse oculaire, par ex. néovascularisation liée au cancer ; et des maladies oculaires, telles que la dégénérescence maculaire liée à l'âge et la néovascularisation cornéenne; l'athérosclérose ; des maladies métaboliques, telles que le diabète ; l'asthme et d'autres maladies pulmonaires interstitielles, y compris le syndrome de Hermansky-Pudlak, le mésothéliome ; des troubles hépatiques, tels que la stéatohépatite non alcoolique.
 






Cited references

REFERENCES CITED IN THE DESCRIPTION



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Patent documents cited in the description




Non-patent literature cited in the description