(19)
(11)EP 3 252 058 B1

(12)EUROPEAN PATENT SPECIFICATION

(45)Mention of the grant of the patent:
20.01.2021 Bulletin 2021/03

(21)Application number: 17177433.4

(22)Date of filing:  11.07.2014
(51)International Patent Classification (IPC): 
C07D 498/14(2006.01)
A61K 31/553(2006.01)
A61P 31/18(2006.01)
C07D 471/14(2006.01)
A61K 31/4995(2006.01)

(54)

POLYCYCLIC-CARBAMOYLPYRIDONE COMPOUNDS AND THEIR USE FOR THE TREATMENT OF HIV INFECTIONS

POLYCYCLISCHE CARBAMOYLPYRIDONVERBINDUNGEN UND DEREN VERWENDUNG ZUR BEHANDLUNG VON HIV-INFEKTIONEN

COMPOSÉS POLYCYCLIQUES-CARBAMOYLPYRIDONE ET LEUR UTILISATION POUR LE TRAITEMENT DES INFECTIONS PAR VIH


(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30)Priority: 12.07.2013 US 201361845806 P

(43)Date of publication of application:
06.12.2017 Bulletin 2017/49

(62)Application number of the earlier application in accordance with Art. 76 EPC:
14745052.2 / 3019503

(73)Proprietor: Gilead Sciences, Inc.
Foster City, CA 94404 (US)

(72)Inventors:
  • JI, Mingzhe
    Union City, CA 94587 (US)
  • LAZERWITH, Scott E.
    Foster City, CA 94404 (US)
  • PYUN, Hyung-Jung
    Foster City, CA 94404 (US)

(74)Representative: Carpmaels & Ransford LLP 
One Southampton Row
London WC1B 5HA
London WC1B 5HA (GB)


(56)References cited: : 
WO-A1-2006/116764
WO-A1-2014/100323
  
  • TAKASHI KAWASUJI ET AL: "Carbamoyl Pyridone HIV-1 Integrase Inhibitors. 2. Bi- and Tricyclic Derivatives Result in Superior Antiviral and Pharmacokinetic Profiles", JOURNAL OF MEDICINAL CHEMISTRY, vol. 56, no. 3, 14 January 2013 (2013-01-14), pages 1124-1135, XP055138745, ISSN: 0022-2623, DOI: 10.1021/jm301550c
  
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).


Description

BACKGROUND


Field



[0001] Compounds, compositions, and methods for the treatment of human immunodeficiency virus (HIV) infection are disclosed. In particular, novel polycyclic carbamoylpyridone compounds and methods for their preparation and use as therapeutic or prophylactic agents are disclosed.

Description of the Related Art



[0002] Human immunodeficiency virus infection and related diseases are a major public health problem worldwide. Human immunodeficiency virus type 1 (HIV-1) encodes three enzymes which are required for viral replication: reverse transcriptase, protease, and integrase. Although drugs targeting reverse transcriptase and protease are in wide use and have shown effectiveness, particularly when employed in combination, toxicity and development of resistant strains have limited their usefulness (Palella, et al. N. Engl. J Med. (1998) 338:853-860; Richman, D. D. Nature (2001) 410:995-1001).

[0003] A goal of antiretroviral therapy is to achieve viral suppression in the HIV infected patient. Current treatment guidelines published by the United States Department of Health and Human Services provide that achievement of viral suppression requires the use of combination therapies, i.e., several drugs from at least two or more drug classes. (Panel on Antiretroviral Guidelines for Adults and Adolescents. Guidelines for the use of antiretroviral agents in HIV-1-infected adults and adolescents. Department of Health and Human Services. Available at http://aidsinfo.nih.gov/ContentFiles/AdultandAdolescentGL.pdf. Section accessed March 14, 2013.) In addition, decisions regarding the treatment of HIV infected patients are complicated when the patient requires treatment for other medical conditions (Id. at E-12). Because the standard of care requires the use of multiple different drugs to suppress HIV, as well as to treat other conditions the patient may be experiencing, the potential for drug interaction is a criterion for selection of a drug regimen. As such, there is a need for antiretroviral therapies having a decreased potential for drug interactions.

[0004] Accordingly, there is a need for new agents that inhibit the replication of HIV and that minimize the potential for drug-drug interactions when co-administered with other drugs.

BRIEF SUMMARY



[0005] The present invention is directed to pharmaceutical compositions comprising novel polycyclic carbamoylpyridone compounds, having antiviral activity, including stereoisomers and pharmaceutically acceptable salts thereof, and one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148, and such compositions for use in the treatment of HIV infections. The compositions of the invention may be used to inhibit the activity of HIV integrase and may be used to reduce HIV replication.

[0006] In one embodiment of the present invention, pharmaceutical compositions comprising a compound having the following Formula (I) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof, and
one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogens;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl;

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.



[0007] In one embodiment of the present invention, pharmaceutical compositions comprising a compound having the following Formula (Ia) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof, and
one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148,wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.



[0008] In one embodiment of the present invention, pharmaceutical compositions comprising a compound having the following Formula (Ib) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof, and
one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.

[0009] In one embodiment of the present invention, pharmaceutical compositions comprising a compound having the following Formula (Ic) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof, and
one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.

[0010] In one embodiment of the present invention, pharmaceutical compositions comprising a compound having the following Formula (Id) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof, and
one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.

[0011] In one embodiment of the present invention, pharmaceutical compositions comprising a compound having the following Formula (Ie) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof, and
one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.

[0012] The invention also provides a pharmaceutical composition as described hereinabove for use in the treatment of an HIV infection in a human being having or at risk of having the infection.

[0013] In yet another embodiment pharmaceutical compositions comprising compounds having the following structures are provided:























or



[0014] In another embodiment, a pharmaceutical composition comprising a compound of Formula (I), (Ia), (Ib), (Ic), (Id), or (Ie) as described herein, or a pharmaceutically acceptable salt thereof, and one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148, is disclosed for use in the treatment of an HIV infection in a human being having or at risk of having the infection.

DETAILED DESCRIPTION


Definitions



[0015] Unless the context requires otherwise, throughout the present specification and claims, the word "comprise" and variations thereof, such as, "comprises" and "comprising" are to be construed in an open, inclusive sense, that is as "including, but not limited to".

[0016] Reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, structure or characteristic described in connection with the embodiment is included in at least one embodiment of the present invention. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

[0017] "Hydroxy" or "hydroxyl" refers to the -OH radical.

[0018] "Oxo" refers to the =O substituent.

[0019] "Alkyl" refers to a straight or branched hydrocarbon chain radical consisting solely of carbon and hydrogen atoms, which is saturated having from one to twelve carbon atoms (C1-C12 alkyl), preferably one to eight carbon atoms (C1-C8 alkyl) or one to six carbon atoms (C1-C6 alkyl), and which is attached to the rest of the molecule by a single bond, e.g., methyl, ethyl, n-propyl, 1-methylethyl (iso-propyl), n-butyl, 1,1-dimethylethyl (t-butyl), and the like.

[0020] "Halo" or "halogen" refers to bromo, chloro, fluoro or iodo.

[0021] "Haloalkyl" refers to an alkyl radical, as defined above, that is substituted by one or more halo radicals, as defined above, e.g., trifluoromethyl, difluoromethyl, trichloromethyl, 2,2,2-trifluoroethyl, 1,2-difluoroethyl, 3-bromo-2-fluoropropyl, 1,2-dibromoethyl, and the like.

[0022] The invention disclosed herein is also meant to encompass pharmaceutical compositions comprising all pharmaceutically acceptable compounds of Formulas (I), (Ia), (Ib), (Ic), (Id), and (Ie), being isotopically-labeled by having one or more atoms replaced by an atom having a different atomic mass or mass number. Examples of isotopes that can be incorporated into the disclosed compounds include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine, chlorine, and iodine, such as 2H, 3H, 11C, 13C, 14C, 13N, 15N, 15O, 17O, 18O, 31P, 32P, 35S, 18F, 36Cl, 123I, and 125I, respectively. These radiolabeled compounds could be useful to help determine or measure the effectiveness of the compounds, by characterizing, for example, the site or mode of action, or binding affinity to pharmacologically important site of action. Certain isotopically-labeled compounds of Formulas (I), (Ia), (Ib), (Ic), (Id), and (Ie), for example, those incorporating a radioactive isotope, are useful in drug and/or substrate tissue distribution studies. The radioactive isotopes tritium, i.e. 3H, and carbon-14, i.e. 14C, are particularly useful for this purpose in view of their ease of incorporation and ready means of detection.

[0023] Substitution with heavier isotopes such as deuterium, i.e. 2H, may afford certain therapeutic advantages resulting from greater metabolic stability. For example, in vivo half-life may increase or dosage requirements may be reduced. Thus, heavier isotopes may be preferred in some circumstances.

[0024] Substitution with positron emitting isotopes, such as 11C, 18F, 15O and 13N, can be useful in Positron Emission Topography (PET) studies for examining substrate receptor occupancy. Isotopically-labeled compounds of Formulas (I), (Ia), (Ib), (Ic), (Id), and (Ie), can generally be prepared by conventional techniques known to those skilled in the art or by processes analogous to those described in the Examples as set out below using an appropriate isotopically-labeled reagent in place of the non-labeled reagent previously employed.

[0025] Also disclosed herein are the in vivo metabolic products of the disclosed compounds. Such products may result from, for example, the oxidation, reduction, hydrolysis, amidation, esterification, and the like of the administered compound, primarily due to enzymatic processes. Accordingly, the disclosure includes compounds produced by a process comprising administering a compound found in a pharmaceutical composition of this invention to a mammal for a period of time sufficient to yield a metabolic product thereof. Such products are typically identified by administering a radiolabeled compound of the invention in a detectable dose to an animal, such as rat, mouse, guinea pig, monkey, or to human, allowing sufficient time for metabolism to occur, and isolating its conversion products from the urine, blood or other biological samples.

[0026] "Stable compound" and "stable structure" are meant to indicate a compound that is sufficiently robust to survive isolation to a useful degree of purity from a reaction mixture, and formulation into an efficacious therapeutic agent.

[0027] "Mammal" includes humans and both domestic animals such as laboratory animals and household pets (e.g., cats, dogs, swine, cattle, sheep, goats, horses, rabbits), and non-domestic animals such as wildlife and the like.

[0028] "Optional" or "optionally" means that the subsequently described event of circumstances may or may not occur, and that the description includes instances where said event or circumstance occurs and instances in which it does not. For example, "optionally substituted aryl" means that the aryl radical may or may not be substituted and that the description includes both substituted aryl radicals and aryl radicals having no substitution.

[0029] "Pharmaceutically acceptable carrier, diluent or excipient" includes without limitation any adjuvant, carrier, excipient, glidant, sweetening agent, diluent, preservative, dye/colorant, flavor enhancer, surfactant, wetting agent, dispersing agent, suspending agent, stabilizer, isotonic agent, solvent, or emulsifier which has been approved by the United States Food and Drug Administration as being acceptable for use in humans or domestic animals.

[0030] Examples of "pharmaceutically acceptable salts" of the compounds disclosed herein include salts derived from an appropriate base, such as an alkali metal (for example, sodium), an alkaline earth metal (for example, magnesium), ammonium and NX4+ (wherein X is C1-C4 alkyl). Pharmaceutically acceptable salts of a nitrogen atom or an amino group include for example salts of organic carboxylic acids such as acetic, benzoic, lactic, fumaric, tartaric, maleic, malonic, malic, isethionic, lactobionic and succinic acids; organic sulfonic acids, such as methanesulfonic, ethanesulfonic, benzenesulfonic and p-toluenesulfonic acids; and inorganic acids, such as hydrochloric, hydrobromic, sulfuric, phosphoric and sulfamic acids. Pharmaceutically acceptable salts of a compound of a hydroxy group include the anion of said compound in combination with a suitable cation such as Na+ and NX4+ (wherein X is independently selected from H or a C1-C4 alkyl group).

[0031] For therapeutic use, salts of active ingredients of the compounds disclosed herein will typically be pharmaceutically acceptable, i.e. they will be salts derived from a physiologically acceptable acid or base. However, salts of acids or bases which are not pharmaceutically acceptable may also find use, for example, in the preparation or purification of a compound of Formula (I), (Ia), (Ib), (Ic), (Id), (Ie), or another compound found in the pharmaceutical compositions of the invention.

[0032] Metal salts typically are prepared by reacting the metal hydroxide with a compound of this invention. Examples of metal salts which are prepared in this way are salts containing Li+, Na+, and K+. A less soluble metal salt can be precipitated from the solution of a more soluble salt by addition of the suitable metal compound.

[0033] In addition, salts may be formed from acid addition of certain organic and inorganic acids, e.g., HCl, HBr, H2SO4, H3PO4 or organic sulfonic acids, to basic centers, typically amines. Finally, it is to be understood that the compositions herein comprise compounds disclosed herein in their un-ionized, as well as zwitterionic form, and combinations with stoichiometric amounts of water as in hydrates.

[0034] Often crystallizations produce a solvate of the compound of the invention. As used herein, the term "solvate" refers to an aggregate that comprises one or more molecules of a compound of the invention with one or more molecules of solvent. The solvent may be water, in which case the solvate may be a hydrate. Alternatively, the solvent may be an organic solvent. Thus, the compounds found in the pharmaceutical compositions of the present invention may exist as a hydrate, including a monohydrate, dihydrate, hemihydrate, sesquihydrate, trihydrate, tetrahydrate and the like, as well as the corresponding solvated forms. The compound found in the pharmaceutical composition of the invention may be a true solvate, while in other cases, the compound found in the pharmaceutical composition of the invention may merely retain adventitious water or be a mixture of water plus some adventitious solvent.

[0035] A "pharmaceutical composition" refers to a formulation of a compound of the invention and a medium generally accepted in the art for the delivery of the biologically active compound to mammals, e.g., humans. Such a medium includes all pharmaceutically acceptable carriers, diluents or excipients therefor.

[0036] "Effective amount" or "therapeutically effective amount" refers to an amount of a compound found in the pharmaceutical composition according to the invention, which when administered to a patient in need thereof, is sufficient to effect treatment for disease-states, conditions, or disorders for which the compounds have utility. Such an amount would be sufficient to elicit the biological or medical response of a tissue system, or patient that is sought by a researcher or clinician. The amount of a compound found in the pharmaceutical composition according to the invention which constitutes a therapeutically effective amount will vary depending on such factors as the compound and its biological activity, the composition used for administration, the time of administration, the route of administration, the rate of excretion of the compound, the duration of the treatment, the type of disease-state or disorder being treated and its severity, drugs used in combination with or coincidentally with the compound in the pharmaceutical composition of the invention, and the age, body weight, general health, sex and diet of the patient. Such a therapeutically effective amount can be determined routinely by one of ordinary skill in the art having regard to their own knowledge, the state of the art, and this disclosure.

[0037] The term "treatment" as used herein is intended to mean the administration of a composition according to the present invention to alleviate or eliminate symptoms of HIV infection and/or to reduce viral load in a patient. The term "treatment" also encompasses the administration of a composition according to the present invention post-exposure of the individual to the virus but before the appearance of symptoms of the disease, and/or prior to the detection of the virus in the blood, to prevent the appearance of symptoms of the disease and/or to prevent the virus from reaching detectible levels in the blood, and the administration of a composition according to the present invention to prevent perinatal transmission of HIV from mother to baby, by administration to the mother before giving birth and to the child within the first days of life.

[0038] The term "antiviral agent" as used herein is intended to mean an agent (compound or biological) that is effective to inhibit the formation and/or replication of a virus in a human being, including but not limited to agents that interfere with either host or viral mechanisms necessary for the formation and/or replication of a virus in a human being.

[0039] The term "inhibitor of HIV replication" as used herein is intended to mean an agent capable of reducing or eliminating the ability of HIV to replicate in a host cell, whether in vitro, ex vivo or in vivo.

[0040] The compounds of the invention, or their pharmaceutically acceptable salts may contain one or more asymmetric centers and may thus give rise to enantiomers, diastereomers, and other stereoisomeric forms that may be defined, in terms of absolute stereochemistry, as (R)- or (S)- or, as (D)- or (L)- for amino acids. The present invention is meant to include all such possible isomers, as well as their racemic and optically pure forms. Optically active (+) and (-), (R)- and (S)-, or (D)- and (L)- isomers may be prepared using chiral synthons or chiral reagents, or resolved using conventional techniques, for example, chromatography and fractional crystallization. Conventional techniques for the preparation/isolation of individual enantiomers include chiral synthesis from a suitable optically pure precursor or resolution of the racemate (or the racemate of a salt or derivative) using, for example, chiral high pressure liquid chromatography (HPLC). When the compounds described herein contain olefinic double bonds or other centres of geometric asymmetry, and unless specified otherwise, it is intended that the compounds include both E and Z geometric isomers. Likewise, all tautomeric forms are also intended to be included.

[0041] A "stereoisomer" refers to a compound made up of the same atoms bonded by the same bonds but having different three-dimensional structures, which are not interchangeable. The present invention contemplates various stereoisomers and mixtures thereof and includes "enantiomers", which refers to two stereoisomers whose molecules are nonsuperimposeable mirror images of one another.

[0042] A "tautomer" refers to a proton shift from one atom of a molecule to another atom of the same molecule. The pharmaceutical composition of the present invention includes tautomers of any said compounds.

Compounds



[0043] As noted above, in the present invention, pharmaceutical compositions comprising compounds having antiviral activity are provided, the compounds having the following Formula (I)

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogens;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl;

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.

[0044] In one embodiment of the present invention, pharmaceutical compositions comprising compounds having the following Formula (Ia) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.



[0045] In one embodiment of the present invention, pharmaceutical compositions comprising compounds having the following Formula (Ib) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.

[0046] In one embodiment of the present invention, pharmaceutical compositions comprising compounds having the following Formula (Ic) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.

[0047] In one embodiment of the present invention, pharmaceutical compositions comprising compounds having the following Formula (Id) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.

[0048] In one embodiment of the present invention, pharmaceutical compositions comprising compounds having the following Formula (Ie) are provided:

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.

[0049] In another embodiment, X is -O-. In another embodiment, X is -NH-. In another embodiment, X is -CH2-. In another embodiment, X is a bond.

[0050] In another embodiment, Y1 is C1-3alkyl and Y2 is hydrogen. In another embodiment, Y1 is methyl and Y2 is hydrogen. In another embodiment, Y1 is C1-3haloalkyl and Y2 is hydrogen. In another embodiment, Y1 is CF3 and Y2 is hydrogen. In another embodiment, Y1 is hydrogen, methyl or CF3 and Y2 is hydrogen. In another embodiment, Y1 and Y2 are both hydrogen.

[0051] In another embodiment, R1 is substituted with one halogen. In a further embodiment, R1 is 4-fluorophenyl or 2-fluorophenyl.

[0052] In another embodiment, R1 is substituted with two halogens. In a further embodiment, R1 is 2,4-difluorophenyl, 2,3-difluorophenyl, 2,6-difluorophenyl, 3-fluoro-4-chlorophenyl, 3,4-difluorophenyl, 2-fluoro-4-chlorophenyl, or 3,5-difluorophenyl. In still a further embodiment, R1 is 2,4-difluorophenyl.

[0053] In another embodiment, R1 is substituted with three halogens. In a further embodiment, R1 is 2,4,6-trifluorophenyl or 2,3,4-trifluorophenyl. In still a further embodiment, R1 is 2,4,6-trifluorophenyl.

[0054] In another embodiment, each Rb is independently hydrogen. In another embodiment, each Rb is independently halogen. In a further embodiment, each Rb is fluoro.

[0055] Another embodiment is provided comprising a pharmaceutical composition comprising a compound of Formula (I), (Ia), (Ib), (Ic), (Id), or (Ie) or a stereoisomer or pharmaceutically acceptable salt thereof, and one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148, for use in a method of treating or preventing an HIV infection in a human having or at risk of having the infection by administering to the human a therapeutically effective amount of said pharmaceutical composition.

[0056] In another embodiment, a pharmaceutical composition comprising a compound of Formula (I), (Ia), (Ib), (Ic), (Id), (Ie), or a stereoisomer or pharmaceutically acceptable salt thereof, and one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148, is provided for use in the treatment or prevention of an HIV infection in a human having or at risk of having the infection.

Pharmaceutical Compositions



[0057] For the purposes of administration, the compounds described herein are administered are formulated as pharmaceutical compositions. Pharmaceutical compositions of the present invention comprise a compound of Formulas (I), (Ia), (Ib), (Ic), (Id), (Ie), and a pharmaceutically acceptable carrier, diluent or excipient. The compound of Formulas (I), (Ia), (Ib), (Ic), (Id), (Ie), is present in the composition in an amount which is effective to treat a particular disease or condition of interest. The activity of compounds of Formulas (I), (Ia), (Ib), (Ic), (Id), (Ie), can be determined by one skilled in the art, for example, as described in the Examples below. Appropriate concentrations and dosages can be readily determined by one skilled in the art.

[0058] Administration of the pharmaceutical compositions of the invention, can be carried out via any of the accepted modes of administration of agents for serving similar utilities. The pharmaceutical compositions of the invention can be prepared by combining a compound found in the pharmaceutical composition of the invention as defined in the claims with an appropriate pharmaceutically acceptable carrier, diluent or excipient, and may be formulated into preparations in solid, semi-solid, liquid or gaseous forms, such as tablets, capsules, powders, granules, ointments, solutions, suppositories, injections, inhalants, gels, microspheres, and aerosols. Typical routes of administering such pharmaceutical compositions include, without limitation, oral, topical, transdermal, inhalation, parenteral, sublingual, buccal, rectal, vaginal, and intranasal. Pharmaceutical compositions of the invention are formulated so as to allow the active ingredients contained therein to be bioavailable upon administration of the composition to a patient. Compositions that will be administered to a subject or patient take the form of one or more dosage units, where for example, a tablet may be a single dosage unit, and a container of a compound of the invention in aerosol form may hold a plurality of dosage units. Actual methods of preparing such dosage forms are known, or will be apparent, to those skilled in this art; for example, see Remington: The Science and Practice of Pharmacy, 20th Edition (Philadelphia College of Pharmacy and Science, 2000). The composition to be administered will, in any event, contain a therapeutically effective amount of a compound found in the pharmaceutical composition of the invention, or a pharmaceutically acceptable salt thereof, for treatment of a disease or condition of interest in accordance with the teachings of this invention.

[0059] The pharmaceutical compositions of the invention may be prepared by methodology well known in the pharmaceutical art. For example, a pharmaceutical composition intended to be administered by injection can be prepared by combining a compound of the invention with sterile, distilled water so as to form a solution. A surfactant may be added to facilitate the formation of a homogeneous solution or suspension. Surfactants are compounds that non-covalently interact with the compound of the invention so as to facilitate dissolution or homogeneous suspension of the compound in the aqueous delivery system.

[0060] The compounds found in the pharmaceutical compositions of the invention, as defined in the claims, or their pharmaceutically acceptable salts, are administered in a therapeutically effective amount, which will vary depending upon a variety of factors including the activity of the specific compound employed; the metabolic stability and length of action of the compound; the age, body weight, general health, sex, and diet of the patient; the mode and time of administration; the rate of excretion; the drug combination; the severity of the particular disorder or condition; and the subject undergoing therapy.

Combination Therapy



[0061] In one embodiment, a pharmaceutical composition comprising a compound disclosed herein in combination with a therapeutically effective amount of one or more (e.g. one or two) additional therapeutic agents is provided for use in a method for treating or preventing an HIV infection in a human having or at risk of having the infection, wherein said method comprises administering to the human a therapeutically effective amount of said composition.

[0062] In one embodiment, a pharmaceutical composition comprising a compound defined herein in combination with a therapeutically effective amount of one or more (e.g. one or two) additional therapeutic agents is provided for use in a method for treating an HIV infection in a human having or at risk of having the infection, wherein said method comprises administering to the human a therapeutically effective amount of said composition.

[0063] Pharmaceutical compositions comprising a compound disclosed herein, or a pharmaceutically acceptable salt thereof, in combination with one or more (e.g. one or two) additional therapeutic agents, and a pharmaceutically acceptable carrier, diluent or excipient are provided.

[0064] The additional therapeutic agents are selected from the group consisting of GS-9131 and GS-9148.

[0065] In certain embodiments, a compound disclosed herein, or a pharmaceutically acceptable salt thereof, is combined with one or more of said additional therapeutic agents. In certain embodiments, a compound disclosed herein, or a pharmaceutically acceptable salt thereof, is combined with two of said additional therapeutic agents.

[0066] In certain embodiments, a compound disclosed herein is combined with one or more additional therapeutic agents in a unitary dosage form for simultaneous administration to a patient, for example as a solid dosage form for oral administration.

[0067] The following Examples illustrate various methods of making compounds found in the pharmaceutical compositions of this invention, i.e., compound of Formula (I):

wherein R1, X, W, Y1, Y2, or L are as defined above. It is understood that one skilled in the art may be able to make these compounds by similar methods or by combining other methods known to one skilled in the art. It is also understood that one skilled in the art would be able to make, in a similar manner as described below, other compounds of Formulas (I), (Ia), (Ib), (Ic), (Id), (Ie), not specifically illustrated below by using the appropriate starting components and modifying the parameters of the synthesis as needed. In general, starting components may be obtained from sources such as Sigma Aldrich, Lancaster Synthesis, Inc., Maybridge, Matrix Scientific, TCI, and Fluorochem USA, etc. or synthesized according to sources known to those skilled in the art (see, for example, Advanced Organic Chemistry: Reactions, Mechanisms, and Structure, 5th edition (Wiley, December 2000)) or prepared as described herein.

[0068] The following examples are provided for purposes of illustration, not limitation.

EXAMPLES


REPRESENTATIVE COMPOUNDS


Example 1


Preparation of Compound 1


(1aS,2S,3aR,12R,12aR)-N-((S)-1-(2,4-difluorophenyl)-2,2,2-trifluoroethyl)-9-hydroxy-8,10-dioxo-1a,2,3a,4,8,10,12,12a-octahydro-1H-2,12-methanocyclopropa[e]pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazepine-7-carboxamide



[0069] 


Step 1



[0070] A 50-mL 1-neck round bottom flask was charged with reactant 1-A (0.11 g, 0.22 mmol) in acetonitrile (1.5 mL) and acetic acid (0.2 mL) was treated with methanesulfonic acid (0.05 mL), sealed with a yellow cap, and heated to 70 °C. After 16 hours, the mixture was cooled to afford a crude solution of intermediate 1-B. LCMS-ESI+ (m/z): [M+H]+calculated for C18H19F2N2O7: 481; found: 481.

Step 2



[0071] The crude mixture from the previous step contains reactant 1-B in acetonitrile (1.5 mL) and acetic acid (0.2 mL). 1-C (WO2013090929A1, 0.032 g, 0.22 mmol) and K2CO3 (0.15 g, 1.1 mmol) were added to the reaction mixture. The reaction mixture was sealed and heated to 70 °C. After 3 hours, the reaction mixture was diluted with EtOAc (50 mL), washed with saturated NaHCO3 and dried over Na2SO4. After concentration, the crude was purified by column chromatography on silica gel with hexane-EtOAc to obtain 1-D. LCMS-ESI+ (m/z): [M+H]+ calculated for C21H20F2N3O5: 526; found: 526.

Step 3



[0072] A 50-mL 1-neck round bottom flask was charged with reactant 1-D (0.02 g, 0.038 mmol) and magnesium bromide (0.02 g, 0.10mmol) in acetonitrile (2 mL). The reaction mixture was heated to 50 °C. After 10 minutes, the reaction mixture was cooled to 0 °C and 1 N hydrochloric acid (0.5 mL) was added in. There were some solids formed and stuck on the flask wall. Add more water (∼ 5 mL). The solid was filtrated and washed with water. Then the solid was transferred to the barcode vial and under lyophilization overnight to afford compound 1. 1H NMR (400 MHz, Chloroform-d) δ 12.38 (s, 1H), 11.25 (d, J = 9.3 Hz, 1H), 8.24 (s, 1H), 7.48 (q, J = 7.8 Hz, 1H), 7.06 - 6.69 (m, 2H), 6.30 - 5.98 (m, 1H), 5.85 (s, 1H), 4.18 (s, 1H), 3.93 (d, J = 34.6 Hz, 2H), 2.04 - 1.35 (m, 5H), 0.80 (d, J = 7.3 Hz, 1H), 0.63 - 0.43 (m, 1H). 19F NMR (377 MHz, Chloroform-d) δ - 75.29 (t, J = 7.5 Hz, 3 F), -107.18 - -109.52 (m, IF), -113.01 (m, IF). LCMS-ESI+ (m/z): [M+H]+calculated for C21H20F2N3O5: 512.; found: 512.

Example 2


Preparation of Compound 2


(1aR,2R,3aS,12S,12aS)-N-((S)-1-(2,4-difluorophenyl)-2,2,2-trifluoroethyl)-9-hydroxy-8,10-dioxo-1a,2,3a,4,8,10,12,12a-octahydro-1H-2,12-methanocyclopropa[e]pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazepine-7-carboxamide



[0073] 


Step 1



[0074] A 50-mL 1-neck round bottom flask was charged with reactant 1-A (0.11 g, 0.22 mmol) in acetonitrile (1.5 mL) and acetic acid (0.2 mL) was treated with methanesulfonic acid (0.05 mL), sealed with a yellow cap, and heated to 70 °C. After 16 hours, the mixture was cooled to afford a crude solution of intermediate 1-B. LCMS-ESI+ (m/z): [M+H]+calculated for C18H19F2N2O7: 481; found: 481.

Step 2



[0075] The crude mixture from the previous step contains reactant 1-B in acetonitrile (1.5 mL) and acetic acid (0.2 mL). 2-A (0.032 g, 0.22 mmol) and K2CO3 (0.15 g, 1.1 mmol) were added to the reaction mixture. The reaction mixture was sealed and heated to 70 °C. After 3 hours, the reaction mixture was diluted with EtOAc (50 mL), washed with saturated NaHCO3 and dried over Na2SO4. After concentration, the crude was purified by column chromatography on silica gel with hexane-EtOAc to obtain 2-B. LCMS-ESI+ (m/z): [M+H]+calculated for C21H20F2N3O5: 526; found: 526.

Step 3



[0076] A 50-mL 1-neck round bottom flask was charged with reactant 2-B (0.03 g, 0.058 mmol) and magnesium bromide (0.03 g, 0.15 mmol) in acetonitrile (2 mL). The reaction mixture was heated to 50 °C. After 10 minutes, the reaction mixture was cooled to 0 °C and 1 N hydrochloric acid (0.5 mL) was added in. There were some solid formed and stuck on the flask wall. Add more water (∼ 5 mL). The solid was filtrated and washed with water. Then the solid was transferred to the barcode vial and under lyophilization overnight to afford compound 2. 1H NMR (400 MHz, Chloroform-d) δ 12.44 (s, 1H), 11.32 (d, J = 9.4 Hz, 1H), 8.29 (s, 1H), 7.81 - 7.39 (m, 1H), 7.19 - 6.67 (m, 2H), 6.42 - 6.04 (m, 1H), 5.94 (d, J = 9.3 Hz, 1H), 4.84 - 4.43 (m, 1H), 4.26 (d, J = 12.6 Hz, 1H), 4.02 (t, J = 10.5 Hz, 1H), 2.08 - 1.38 (m, 5H), 0.88 (q, J = 7.2 Hz, 1H), 0.60 (dd, J = 6.3, 3.3 Hz, 1H). 19F NMR (377 MHz, Chloroform-d) δ -75.25 (t, J = 6.5 Hz, 3 F), -106.94--109.63 (m, IF), -112.11 (m, IF). LCMS-ESI+ (m/z): [M+H]+ calculated for C21H20F2N3O5: 512.; found: 512.

Example 3


Preparation of Compound 3


(1aS,2S,3aR,12R,12aR)-N-((R)-1-(2,4-difluorophenyl)ethyl)-9-hydroxy-8,10-dioxo-1a,2,3a,4,8,10,12,12a-octahydro-1H-2,12-methanocyclopropa[e]pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazepine-7-carboxamide



[0077] 




Step 1



[0078] A 50-mL 1-neck round bottom flask was charged with reactant 3-A (0.11 g, 0.24 mmol) in acetonitrile (1.5 mL) and acetic acid (0.2 mL) was treated with methanesulfonic acid (0.05 mL), sealed with a yellow cap, and heated to 70 °C. After 16 hours, the mixture was cooled to afford a crude solution of intermediate 3-B. LCMS-ESI+ (m/z): [M+H]+calculated for C18H19F2N2O7: 427; found: 427.

Step 2



[0079] The crude mixture from the previous step contains reactant 3-B in acetonitrile (1.5 mL) and acetic acid (0.2 mL). 1-C (0.036 g, 0.24 mmol) and K2CO3 (0.167 g, 1.2 mmol) were added to the reaction mixture. The reaction mixture was sealed and heated to 70 °C. After 3 hours, the reaction mixture was diluted with EtOAc (50 mL), washed with saturated NaHCO3 and dried over Na2SO4. After concentration, the crude was purified by column chromatography on silica gel with hexane-EtOAc to obtain 3-C. LCMS-ESI+ (m/z): [M+H]+calculated for C21H20F2N3O5: 472; found: 472.

Step 3



[0080] A 50-mL 1-neck round bottom flask was charged with reactant 3-C (0.03 g, 0.058 mmol) and magnesium bromide (0.03 g, 0.15 mmol) in acetonitrile (2 mL). The reaction mixture was heated to 50 °C. After 10 minutes, the reaction mixture was cooled to 0 °C and 1 N hydrochloric acid (0.5 mL) was added in. There were some solid formed and stuck on the flask wall. Add more water (∼ 5 mL). The solid was filtrated and washed with water. Then the solid was transferred to the barcode vial and under lyophilization overnight to afford compound 3. 1H NMR (400 MHz, Chloroform-d) δ 12.35 (s, 1H), 10.57 (s, 1H), 8.26 (s, 1H), 7.61 - 7.28 (m, 1H), 7.00 - 6.65 (m, 2H), 5.89 (s, 1H), 5.45 (d, J = 10.2 Hz, 1H), 5.34 - 5.13 (m, 1H), 4.58 (d, J = 2.0 Hz, 1H), 4.20 (s, 1H), 4.02 (d, J = 7.2 Hz, 2H), 2.12 - 1.43 (m, 6H), 0.87 (d, J = 7.5 Hz, 1H), 0.60 (s, 1H).. 19F NMR (376 MHz, Methanol-d4) δ -113.03 (m, IF), -114.92 (m, IF). LCMS-ESI+ (m/z): [M+H]+ calculated for C21H20F2N3O5: 458.; found: 458.

Example 4


Preparation of Compound 4


(1aR,2R,3aS,12S,12aS)-N-((R)-1-(2,4-difluorophenyl)ethyl)-9-hydroxy-8,10-dioxo-1a,2,3a,4,8,10,12,12a-octahydro-1H-2,12-methanocyclopropa[e]pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazepine-7-carboxamide



[0081] 


Step 1



[0082] A 50-mL 1-neck round bottom flask was charged with reactant 3-A (0.11 g, 0.24 mmol) in acetonitrile (1.5 mL) and acetic acid (0.2 mL) was treated with methanesulfonic acid (0.05 mL), sealed with a yellow cap, and heated to 70 °C. After 16 hours, the mixture was cooled to afford a crude solution of intermediate 3-B. LCMS-ESI+ (m/z): [M+H]+calculated for C18H19F2N2O7: 427; found: 427.

Steps 2



[0083] The crude mixture from the previous step contains reactant 3-B in acetonitrile (1.5 mL) and acetic acid (0.2 mL). 2-A (0.036 g, 0.24 mmol) and K2CO3 (0.167 g, 1.2 mmol) were added to the reaction mixture. The reaction mixture was sealed and heated to 70 °C. After 3 hours, the reaction mixture was diluted with EtOAc (50 mL), washed with sat NaHCO3 and dried over Na2SO4. After concentration, the crude was purified by column chromatography on silica gel with hexane-EtOAc to obtain 4-A. LCMS-ESI+ (m/z): [M+H]+calculated for C21H20F2N3O5: 472; found: 472.

Steps 3



[0084] A 50-mL 1-neck round bottom flask was charged with reactant 4-A (0.03 g, 0.058 mmol) and magnesium bromide (0.03 g, 0.15mmol) in acetonitrile (2 mL). The reaction mixture was heated to 50 °C. After 10 minutes, the reaction mixture was cooled to 0 °C and 1 N hydrochloric acid (0.5 mL) was added in. There were some solid formed and stuck on the flask wall. Add more water (∼ 5 mL). The solid was filtrated and washed with water. Then the solid was transferred to the barcode vial and under lyophilization overnight to afford compound 4. 1H NMR (400 MHz, Chloroform-d) δ 12.35 (s, 1H), 10.56 (s, 1H), 8.26 (s, 1H), 7.37 (s, 1H), 7.00 - 6.63 (m, 2H), 5.89 (s, 1H), 5.45 (d, J = 11.0 Hz, 1H), 5.34 - 5.00 (m, 1H), 4.58 (d, J = 2.4 Hz, 1H), 4.21 (s, 1H), 4.03 (s, 2H), 2.10 - 1.43 (m, 6H), 0.87 (d, J = 7.5 Hz, 1H), 0.60 (s, 1H). 19F NMR (376 MHz, Chloroform-d) δ -113.00 (m, IF), -115.00 (m, IF). LCMS-ESI+ (m/z): [M+H]+ calculated for C21H20F2N3O5: 458.; found: 458.

Example 5


Preparation of Compound 5


(1aR,2R,12S,12aS)-N-(2,4-difluorobenzyl)-1,1-difluoro-9-hydroxy-8,10-dioxo-1a,2,3a,4,8,10,12,12a-octahydro-1H-2,12-methanocyclopropa[e]pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazepine-7-carboxamide



[0085] 


Step 1



[0086] A mixture of compound 5-A (1252 mg, 6.796 mmol), phthalimide (1631 mg, 11.09 mmol), and PPh3 (3939 mg, 15.02 mmol) in THF (75 mL) was stirred at 0 °C bath as DIAD (3.0 mL, 15.24 mmol) was added. After addition, the mixture was stirred at room temperature. After 3 hours, the mixture was concentrated and the residue was triturated with ethyl ether (∼100 mL) at 0 °C bath for 10 minutes before filtration. After the filtrate was concentrated, the residue was dissolved in ethyl ether (∼50 ml) again and the insoluble material was filtered off. The filtrate was concentrated, and the residue was purified by CombiFlash using hexanes-ethyl acetate as eluents to obtain compound 5-B. 1H NMR (400 MHz, CDCl3) δ 7.91 - 7.75 (m, 2H), 7.75 - 7.64 (m, 2H), 6.06 (dt, J = 5.8, 2.0 Hz, 1H), 5.99 (dt, J = 5.7, 1.7 Hz, 1H), 5.64 (ddq, J = 7.4, 5.5, 1.6 Hz, 1H), 5.25 (tq, J = 6.7, 2.1 Hz, 1H), 2.90 (dt, J = 13.6, 8.1 Hz, 1H), 2.22 - 2.00 (m, 1H), 1.21 (d, J = 1.3 Hz, 9H).

Step 2



[0087] A mixture of compound 5-B (750 mg, 2.394 mmol) and NaF (1.0 mg, 0.024 mmol) in toluene (1 mL) was stirred at 110 °C as FSO2CF2COOTMS (0.95 mL, 4.821 mmol) was added using syringe drive over 5 hours. The reaction mixture was treated with NaHCO3 solution and the organic soluble material was extracted with CH2Cl2 (x 2). After the combined extracts were dried (Na2SO4) and concentrated, the residue was separated with Combiflash using hexanes-ethyl acetate as eluents to get partially pure compound 5-C and the reactant.

[0088] The recovered reactant (577 mg) with NaF (1.0 mg, 0.024 mmol) in toluene (1 mL) was again stirred at 110 °C as FSO2CF2COOTMS (3 mL, 15.22 mmol) was added using syringe drive over 15 hours. The reaction mixture was worked up as described previously and the residue was purified by Combiflash using hexanes-ethyl acetate as eluents to get partially pure compound 5-C (205 mg). Two partially pure compound 5-C were combined and purified again by CombiFlash using hexanes- ethyl acetate as eluents to get compound 5-C. 1H NMR (400 MHz, CDCl3) δ 7.95 - 7.81 (m, 2H), 7.82 - 7.67 (m, 2H), 5.26 (d, J = 6.5 Hz, 1H), 4.99 - 4.84 (m, 1H), 2.67 (ddd, J = 35.4, 14.5, 8.0 Hz, 3H), 2.13 - 1.91 (m, 1H), 1.15 (d, J= 0.9 Hz, 9H). 19F NMR (376 MHz, Chloroform-d) δ -126.81 (dt, J = 170.9, 14.5 Hz, 1 F), -129.43 - -130.54 (m, 0.15F), -137.42 - -138.86 (m, 0.15F), -148.12 (dt, J = 171.0, 4.2 Hz, 1 F), -150.85 ∼ -152.25 (m, 0.15F).

Step 3



[0089] A solution of compound 5-C (330 mg, 0.908 mmol) and hydrazine hydrate (0.18 mL, 3.7 mmol) in ethanol (10 mL) was stirred at 70 °C bath for 2 hours. After being cooled to room temperature, the mixture was diluted with ethyl ether (30 mL) and the solids filtered off. After the filtrate was concentrated, the residue was triturated with CH2Cl2, and filtered off some solids present. After the filtrate was concentrated, compound 5-D was obtained. 1H NMR (400 MHz, CDCl3) δ 5.29 - 5.24 (m, 1H), 3.65 - 3.51 (m, 1H), 2.41 - 2.09 (m, 4H), 1.93 - 1.52 (m, 2H), 1.21 (s, 9H). 19F NMR (376.1 MHz, CDCl3) δ -124.67 (dt, J = 172.1, 15.1 Hz, IF), -126.97 (d, J = 14.8 Hz, 0.1F), - 129.38 (dt, J = 150.2, 11.8 Hz, 0.1F), -147.22 (dt, J = 172.2, 4.6 Hz, IF), -155.11 (dd, J = 149.9, 2.6 Hz, 0.1F). LCMS-ESI+ (m/z): [M+H]+ calculated for C11H18F2NO2: 234.13; found: 233.9.

Step 4



[0090] A solution of compound 5-D (205 mg, 0.879 mmol) in 1 N KOH (3 mL), THF (3 mL), and water (3 mL) was stirred at 50 °C for 16 hours before concentration to ∼1/3 volume. The resulting solution was cooled to 0 °C and neutralized with IN HCl (∼3.2 mL). After the solution was diluted with saturated NaHCO3 (3 mL) and THF (5 mL), the solution was stirred at 0 °C and as Boc2O (613 mg, 2.809 mmol) was added. After 2 hours, additional Boc2O (450 mg, 2.062 mmol) was added. After 1.5 hours more at 0 °C, the mixture was diluted with water and extracted with ethyl acetate (x 2). The extracts were washed with water (x 1), combined, dried (Na2SO4), and concentrated. The residue was purified by CombiFlash using hexanes- ethyl acetate as eluents to get compound 5-E. 1H NMR (400 MHz, CDCl3) δ 5.27 (br, 1H), 4.51 - 4.38 (d, J = 7.4 Hz, 1H), 4.17 (d, J = 7.4 Hz, 1H), 2.88 (br, 1H), 2.24 (m, 3H), 1.84 - 1.70 (m, 1H), 1.44 (s, 9H). 19F NMR (376.1 MHz, CDCl3) δ -125.18 (d, J = 172.5 Hz, IF), -147.57 (d, J = 171.8 Hz, IF). LCMS-ESI+ (m/z): [M+H]+ calculated for C11H18F2NO2: 234.13; found: 233.9.

Step 5



[0091] A solution of compound 5-E (173 mg, 0.694 mmol) in CH2Cl2 (2 mL) was stirred at room temperature as 4 N HCl in dioxane (2 mL) was added. After 1 hour, additional 4 N HCl in dioxane (2 mL) was added and the resulting mixture was stirred at room temperature for 1 hour. The mixture was concentrated and the residue was co-evaporated with toluene (x 1) before drying in high vacuum for 1 hour.

[0092] A mixture of the resulting residue, compound 5-F (285 mg, 0.691 mmol), and K2CO3 (191 mg, 1.382 mmol) in MeCN (2.7 mL) and AcOH (0.3 mL) was stirred at 90 °C bath. After 2 hours, the reaction mixture was stirred at 0 °C, quenched with 1 N HCl (∼ 4 mL), and diluted with water before extraction with CH2Cl2 (x 3). The combined extracts were dried (Na2SO4), and concentrated. The residue was purified by preparative HPLC to get 67 mg of the partially purified cyclic product.

[0093] To a solution of the partially purified cyclic product in MeCN (3 mL) was added MgBr2 (65 mg, 0.353 mmol) and the resulting mixture was stirred at 50 °C for 1 hour, and cooled to 0 °C before addition of 1 N HCl. After the mixture was diluted with water, the product was extracted with CH2Cl2 (x 3) and the combined extracts were dried (Na2SO4), and concentrated. The residue was purified by preparative HPLC and the product containing fraction was freeze-dried to get compound 5 as a 1:1 mixture with TFA. 1H NMR (400 MHz, CDCl3) δ 10.48 (t, J = 6.0 Hz, 1H), 8.49 (s, 1H), 7.43 - 7.29 (m, 1H), 6.91 - 6.73 (m, 2H), 5.80 (dd, J = 9.8, 4.0 Hz, 1H), 5.47 (t, J = 3.6 Hz, 1H), 4.80 (s, 1H), 4.72 - 4.52 (m, 2H), 4.35 (dd, J = 13.0, 4.1 Hz, 1H), 4.09 (dd, J = 12.9, 9.8 Hz, 1H), 2.50 (dd, J = 14.7, 7.3 Hz, 1H), 2.40 - 2.29 (m, 1H), 2.11 (dq, J = 13.9, 3.4 Hz, 1H), 2.03 - 1.89 (m, 1H). 19F NMR (376.1 MHz, CDCl3) δ -76.38 (s, 3F), -111.32 (p, J = 7.8 Hz, IF), -114.54 (q, J = 8.6 Hz, IF), -117.70 (dt, J = 174.1, 14.5 Hz, IF), -139.72 ∼ - 142.02 (m, IF). LCMS-ESI+ (m/z): [M+H]+calculated for C22H18F4N3O5: 480.12; found: 480.2.

Example 6


Preparation of Compound 6


(1aR,2R,3aS,12S,12aS)-N-(2,4-difluorobenzyl)-9-hydroxy-8,10-dioxo-1a,2,3a,4,8,10,12,12a-octahydro-1H-2,12-methanocyclopropa[e]pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazepine-7-carboxamide



[0094] 


Step 1



[0095] To a solution of compound 6-A (4.272 g, 30.053 mmol), PPh3 (15.785 g, 60.18 mmol), and pivaloic acid (3.5 mL, 30.446 mmol) in THF (200 mL) was stirred at 0 °C as diisopropyl azodicarboxylate (11.9 mL, 60.439 mmol) was added over 5 min. After 10 min, the mixture was warmed to room temperature and stirred for 30 min. The mixture was concentrated and resulting syrup was dissolved in ethyl ether. After the mixture was filtered and the filtrate was concentrated, the residue was purified by CombiFlash to obtain compound 6-B. 1H NMR (400 MHz, CDCl3) δ 6.11 (s, 2H), 5.80 (m, 2H), 2.29 - 2.11 (m, 2H), 2.04 (s, 3H), 1.17 (s, 9H).

Step 2



[0096] A suspension of compound 6-B (4.875 g, 21.545 mmol) and K2CO3 (3.265 g, 23.623 mmol) in methanol (100 mL) was stirred at room temperature for 2 hours. After the reaction mixture was diluted with CH2Cl2 (∼150 mL), the mixture was filtered and the filtrate was concentrated. The residue was triturated with CH2Cl2 and the supernatant was purified by CombiFlash to obtain compound 6-C. 1H NMR (400 MHz, CDCl3) δ 6.11 (br d, J = 5.7Hz, 1H), 6.02 (br d, J = 5.7 Hz, 1H), 5.82 - 5.72 (m, 1H), 5.14 - 4.98 (m, 1H), 2.28 - 2.06 (m, 2H), 1.57 (s, 1H), 1.17 (s, 9H).

Step 3



[0097] A solution of compound 6-C (1.503 g, 8.158 mmol) in CH2Cl2 (50 mL) was stirred at 0 °C as 1 M solution of ZnEt2 in toluene (9 mL) was added. After 15 min, CH2I2 (1.45 mL, 18 mmol) followed by 1 M solution of ZnEt2 in toluene (9 mL) were added. After the mixture was stirred for 30 min, additional CH2I2 (1.45 mL, 18 mmol) was added. After 30 min, the mixture was warmed to room temperature and stirred for 2 hours before additional 1 M solution of ZnEt2 in toluene (9 mL) and CH2I2 (1.45 mL, 18 mmol) were added. The resulting mixture was stirred at room temperature for 18 hours. The reaction mixture was poured into 0 °C cold saturated NH4Cl and the product was extracted with ethyl acetate (x 2). The combined extracts were dried (Na2SO4), and concentrated before purification by CombiFlash to get compound 6-D. 1H NMR (400 MHz, CDCl3) δ 5.12 (d, J = 5.2 Hz, 1H), 4.78 (td, J = 8.3, 4.6 Hz, 1H), 2.00 - 1.87 (m, 1H), 1.75 - 1.65 (m, 1H), 1.56 (s, 1H), 1.51 (m, 1H), 1.38 (m, 1H), 1.19 (s, 9H), 0.58 (m, 2H).

Step 4



[0098] A mixture of compound 6-D (1291 mg, 6.512 mmol) and PPh3 (3794 mg, 14.47 mmol) in THF (70 mL) was stirred at 0 °C bath as DIAD (2.9 mL, 14.73 mmol) was added. After addition, the mixture was stirred at 0 °C for 30 min and then at room temperature overnight. The mixture was concentrated to syrup and dissolved in ether (∼70 mL) and stirred at 0 °C bath for ∼1 hour before filtration. After the filtrate was concentrated, the residue was purified by CombiFlash using hexanes-ethyl acetate as eluents to obtain compound 6-E. 1H NMR (400 MHz, Chloroform-d) δ 7.81 (dd, J = 5.4, 3.0 Hz, 2H), 7.70 (dd, J = 5.5, 3.0 Hz, 2H), 5.15 (d, J = 5.8 Hz, 1H), 4.70 - 4.57 (m, 1H), 2.24 ∼ 2.08 (m, 2H), 1.92 (dt, J = 8.6, 4.4 Hz, 1H), 1.84 (d, J = 16.3 Hz, 1H), 1.05 (s, 9H), 0.81 (tdd, J = 8.6, 5.9, 1.3 Hz, 1H), 0.11 (dt, J = 6.0, 4.0 Hz, 1H).

Step 5



[0099] A solution of compound 6-E (890 mg, 2.719 mmol) and hydrazine hydrate (0.53 mL, 10.89 mmol) in ethanol (15 mL) was stirred at 70 °C bath for 2 hours. After cooled to room temperature, the mixture was diluted with ethyl ether (50 mL) and the resulting mixture was stirred at 0 °C bath for 1 hour before filter the solids. After the filtrate was concentrated, the residue was triturated with CH2Cl2, and filtered off some solids present. After the filtrate was concentrated, compound 6-F was obtained. 1H NMR (400 MHz, CDCl3) δ 5.15 (d, J = 5.2 Hz, 1H), 3.33 (d, J = 6.2 Hz, 1H), 1.90 (br, 2H), 1.77 (dt, J = 15.8, 5.7 Hz, 1H), 1.64 - 1.55 (m, 2H), 1.55 - 1.47 (m, 1H), 1.20 (s, 9H), 0.60 - 0.48 (m, 1H), -0.01 (dt, J = 5.9, 3.8 Hz, 1H).

Step 6



[0100] A solution of compound 6-F (522 mg, 2.646 mmol) in 1 N KOH (9.1 mL), THF (9 mL), and water (9 mL) was stirred at 50 °C for 15 hours and at 70 °C for 7 hours before concentration to ∼1/3 volume. The resulting solution was cooled to 0 °C and neutralized with IN HCl (∼9.2 mL). After the solution was diluted with saturated NaHCO3 (10 mL) and THF (10 mL), the solution was stirred at 0 °C and as Boc2O (1846 mg, 8.412 mmol) was added. After 1 hour, the mixture was warmed to room temperature and stirred for 15 hours before addition of Boc2O (1846 mg, 8.412 mmol). After 6 hours, the mixture was diluted with water and extracted with ethyl acetate (x 2). The extracts were washed with water (x 1), combined, dried (Na2SO4), and concentrated. The residue was purified by CombiFlash using hexanes- ethyl acetate as eluents to get compound 6-G. 1H NMR (400 MHz, CDCl3) δ 5.3 (br, 1H), 4.26 (d, J = 4.4 Hz, 1H), 4.03 (d, J = 6.3 Hz, 1H), 2.5 (br, 1H), 1.64 (ddd, J = 15.5, 6.4, 4.6 Hz, 1H), 1.59 - 1.49 (m, 3H), 1.42 (s, 9H), 0.60 - 0.37 (m, 1H), -0.08 (dt, J = 5.9, 3.7 Hz, 1H).

Step 7



[0101] A solution of compound 6-G (457 mg, 2.143 mmol) in CH2Cl2 (5.5 mL) was stirred at room temperature as 4 N HCl in dioxane (5.5 mL) was added. After 1 hour, additional 4 N HCl in dioxane (5.5 mL) was added and the resulting mixture was stirred at room temperature for 1 hour. The mixture was concentrated and the residue was dried in high vacuum overnight.

[0102] A mixture of the resulting residue (320 mg), compound 5-F (881 mg, 2.137 mmol), and K2CO3 (592 mg, 4.183 mmol) in MeCN (10 mL) and AcOH (1 mL) was stirred at 65 °C bath. After 3 hours, the reaction mixture was stirred at 0 °C, quenched with 1 N HCl (∼ 2 mL), and diluted with water before extraction with CH2Cl2 (x 3). The combined extracts were dried (Na2SO4), and concentrated. The residue was purified by CombiFlash (40 g column) using hexanes - ethyl acetate - 20% MeOH/ ethyl acetate as eluents to get 433 mg of the partially purified cyclic product.

[0103] To a solution of the partially purified cyclic product in MeCN (5 mL) was added MgBr2 (453 mg, 2.46 mmol) and MeCN (2 mL) at room temperature. The resulting mixture was stirred at 50 °C for 20 min, and cooled to 0 °C before addition of 1 N HCl. After the mixture was diluted with water, the product was extracted with CH2Cl2 (x 3) and the combined extracts were dried (Na2SO4), and concentrated. The residue was purified by CombiFlash using CH2Cl2-20%MeOH/CH2Cl2 as eluents. After the combined product containing fractions were concentrated, the residue was triturated with MeCN (5 mL) for 15 min, filtered, and the solids collected were dried in vacuum to obtain compound 6. 1H NMR (400 MHz, CDCl3) δ 10.51 (t, J = 6.0 Hz, 1H), 8.47 (s, 1H), 7.40 - 7.29 (m, 1H), ∼7 (br, 1H), 6.90 - 6.76 (m, 2H), 5.94 (dd, J = 9.8, 4.0 Hz, 1H), 5.21 (d, J = 3.8 Hz, 1H), 4.63 (dd, J = 5.9, 2.7 Hz, 2H), 4.61 - 4.53 (m, 1H), 4.32 (dd, J = 13.0, 4.1 Hz, 1H), 4.04 (dd, J = 12.9, 9.9 Hz, 1H), 1.86 - 1.64 (m, 2H), 1.61 (p, J = 4.0 Hz, 1H), 1.52 (dt, J = 13.6, 3.4 Hz, 1H), 0.87 (q, J = 7.5 Hz, 1H), 0.60 (dt, J = 6.7, 3.4 Hz, 1H). 19F NMR (376.1 MHz, CDCl3) δ -76.43 (s, 3F), -111.61 (p, J = 7.7 Hz, IF), -114.58 (q, J = 8.5 Hz, IF). LCMS-ESI+ (m/z): [M+H]+ calculated for C22H20F2N3O5: 444.14; found: 444.2.

Example 7


Preparation of Compound 7


(1aS,2S,3aR,12R,12aR)-N-(2,4-difluorobenzyl)-9-hydroxy-8,10-dioxo-1a,2,3a,4,8,10,12,12a-octahydro-1H-2,12-methanocyclopropa[e]pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazepine-7-carboxamide



[0104] 




Step 1



[0105] A 500-mL 1-neck round bottom flask was charged with reactant 7-A (5.0 g, 35 mmol) and DCM (100 ml). The reaction mixture was cooled to 0 °C with stirring. 1 N diethylzinc in hexane (39 ml) was added to the reaction mixture slowly. The reaction mixture was stirred at 0 °C for 15 minutes. Diiodomethane (4.25 mL) was added followed by IN diethylzinc in hexane (39 mL). After stirring another 15 minutes, additional diiodomethane (4.25 ml) was added to the reaction mixture. Then the reaction mixture was warmed to room temperature and stirred for overnight. The reaction mixture was poured onto a cold aqueous solution of NH4Cl and extracted with ethyl acetate. The organic layer was dried and evaporated in vacuo. The residue was purified by column chromatography on silica gel with hexane-EtOAc to afford 7-B. 1H NMR (400 MHz, Chloroform-d) δ 5.34 - 5.02 (m, 1H), 4.45 (ddd, J = 8.7, 7.7, 4.7 Hz, 1H), 2.31 (dt, J = 13.4, 7.8 Hz, 1H), 2.02 (s, 3H), 1.84 - 1.59 (m, 2H), 1.25 - 1.07 (m, 2H), 0.92 (dt, J = 5.4, 3.9 Hz, 1H), 0.54 (td, J = 7.7, 5.5 Hz, 1H).

Step 2



[0106] A 500-mL 1-neck round bottom flask was charged with reactant 7-B (5.5 g, 35 mmol), triphenylphosphine (20.3 g, 77 mmol), phthalimide (8.3 g, 56 mmol) and THF (200 ml). The reaction mixture was cooled to 0 °C with stirring. Diisopropyl azodicarboxylate (DIAD) (15.3 ml, 77 mmol) was added to the reaction mixture slowly. The reaction mixture was stirred at room temperature overnight. The reaction mixture was concentrated down, re-dissolved in ether and stirred at 0 °C for 10 minutes. Solid (triphenylphosphine oxide) was filtered away. The filtrate was concentrated and purified by column chromatography on silica gel with hexane-EtOAc to obtain 7-C. 1H NMR (400 MHz, Acetonitrile-d3) δ 8.06 - 7.61 (m, 4H), 5.84 (d, J = 5.1 Hz, 1H), 4.88 - 4.50 (m, 1H), 2.29 (dd, J = 15.1, 8.7 Hz, 1H), 2.01(s, 3 H), 1.98 (m, 1H), 1.94 (d, J = 2.4 Hz, 1H), 1.49 (m, 1H), 0.68 (td, J = 8.2, 5.5 Hz, 1H), 0.56 (s, 1H).

Steps 3 and 4



[0107] A 250-mL 1-neck round bottom flask was charged with reactant 7-C (1.0 g, 3.5 mmol), hydrazine monohydrate (∼2ml) and EtOH (20 ml). The reaction mixture was stirred at 70 °C for 30 minutes. The reaction mixture was concentrated under high vacuum for 1 hour to afford 7-D. The crude reaction mixture was re-dissolved in THF (20 mL). Saturated NaHCO3 (20 mL) and di-tert-butyl dicarbonate (8g, 36.7 mmol) were added and the reaction mixture was stirred over 24 hours. The reaction mixture was extracted with EtOAc (2 x 100 mL) and dried over Na2SO4. After concentration, the crude was purified by column chromatography on silica gel with hexane-EtOAc to obtain 7-E. 1H NMR (400 MHz, Chloroform-d) δ 4.63 (td, J = 8.3, 4.6 Hz, 1H), 4.01 - 3.81 (m, 1H), 2.17 (s, 1H), 1.81 (dd, J = 14.4, 7.8 Hz, 1H), 1.55 (ddt, J = 8.0, 5.6, 4.2 Hz, 1H), 1.39 (s, 9H), 1.38 - 1.31 (m, 2H), 0.68 - 0.33 (m, 2H).

Step 5



[0108] A 100-mL 1-neck round bottom flask was charged with reactant 7-E (0.5 g, 2.34 mmol), triphenylphosphine (1.35g, 5.1mmol), benzoic acid (0.46g, 3.8m mol) and THF (20 ml). The reaction mixture was cooled to 0 °C with stirring. DIAD (1.01 ml, 5.1 mmol) was added to the reaction mixture slowly. The reaction mixture was stirred at room temperature for overnight. The reaction mixture was concentrated down, re-dissolved in ether and stirred at 0 °C for 10 minutes. Solid (triphenylphosphine oxide) was filtered away. The crude was purified was purified by column chromatography on silica gel with hexane-EtOAc to obtain 7-F. 1H NMR (400 MHz, Chloroform-d) δ 8.16 - 7.77 (m, 2H), 7.58 (dd, J = 7.1, 1.6 Hz, 1H), 7.54 - 7.36 (m, 2H), 4.99 - 4.76 (m, 1H), 4.02 (dt, J = 8.7, 3.2 Hz, 1H), 1.70 (d, J = 3.3 Hz, 1H), 1.62 (ddd, J = 8.7, 5.1, 3.6 Hz, 1H), 1.53 - 1.44 (m, 1H), 1.30 (s, 9H), 0.96 (dd, J = 6.3, 2.6 Hz, 2H), 0.63 - 0.47 (m, 1H), 0.00 (dd, J = 6.3, 3.4 Hz, 1H).

Step 6



[0109] A 100-mL 1-neck round bottom flask was charged with 7-F (0.7 g, 2.2 mmol), THF (10 mL) and MeOH (5 mL). 1 N KOH (4.4 mL) was added to the reaction mixture. The reaction mixture was stirred at room temperature for 30 minutes. After acidification with 1 N HCl to pH = 4, the reaction mixture was extracted with EtOAc (2x 50 ml). The combined organic layers were dried by Na2SO4. After concentration, the crude was purified by column chromatography on silica gel with hexane-EtOAc to obtain the Boc protected product. The Boc protected product in DCM was stirred at room temperature as 5.5 mL of 4 N HCl in dioxane was added in. After stirred at room temperature for 2 hours, the reaction mixture was concentrated and the residue was dried under high vacuum for overnight. The resulting 7-G was used for the next reaction without further purification.

Step 7



[0110] A 100-mL 1-neck round bottom flask was charged with 7-G (0.22 g, 1.47 mmol), H (0.60 g, 1.47 mmol), potassium carbonate (0.40 g, 2.90 mmol), acetic acid (0.71 g, 11.83 mmol) and acetonitrile (10 mL). The reaction mixture was stirred at 65 °C bath for 2 hours. After cooling to room temperature, the reaction mixture was diluted with EtOAc (100 mL), washed with saturated NaHCO3 and dried over Na2SO4. After concentration, the crude was purified by column chromatography on silica gel with hexane-EtOAc to obtain 7-1. [M+H]+calculated for C21H20F2N3O5: 458.; found: 458.

Step 8



[0111] A 50-mL 1-neck round bottom flask was charged with 7-1 (0.20 g, 0.44 mmol), magnesium bromide (0.21 g, 1.14 mmol) and acetonitrile (5 mL). The resulting mixture was stirred at 50 °C for 10 minutes. Then the mixture was stirred at 0 °C bath and 1 N HCl (∼4 mL) was added, followed by addition of water (∼ 5 mL). The solid was filtered and washed with water. After drying under high vacuum overnight, compound 7 was obtained. 1H NMR (400 MHz, Chloroform-d) δ 12.33 (s, 1H), 10.36 (s, 1H), 8.29 (s, 1H), 7.44 - 7.30 (m, 1H), 6.89 - 6.66 (m, 2H), 5.89 (d, J = 10.0 Hz, 1H), 5.25 - 5.13 (m, 1H), 4.75 - 4.43 (m, 3H), 4.20 (s, 1H), 4.10 - 3.84 (m, 1H), 1.90 - 1.30 (m, 4 H), 0.86 (t, J = 7.5 Hz, 1H), 0.58 (dd, J = 6.6, 3.3 Hz, 1H). 19F NMR (376 MHz, Chloroform-d) δ -112.30 , -114.74 . [M+H]+calculated for C21H20F2N3O5: 444; found: 444.

Example 8


Preparation of Compound 8


(1aS,2S,10aS,11R,11aR)-N-(2,4-difluorobenzyl)-5-hydroxy-4,6-dioxo-1a,2,4,6,10,10a,11,11a-octahydro-1H-2,11-methanocyclopropa[4,5]pyrido[1,2-a]pyrido[1,2-d]pyrazine-7-carboxamide



[0112] 


Step 1



[0113] A mixture of the compound 8-A (1.002 g, 3.894 mmol) and Pd(OAc)2 (15.0 mg, 0.067 mmol) in ether (15 mL) was stirred at 0 °C as diazomethane in ether (10 mL) was added over ∼3 min. After 30 min at 0 °C, additional diazomethane in ether (10 mL) was added and the mixture was stirred at 0 °C for 30 min. The mixture was filtered through celite pad and concentrated. The residue was purified by Combiflash (40 g column) using hexanes-ethyl acetate as eluents to provide compound 8-B. 1H NMR (400 MHz, Chloroform-d) δ 7.35 - 7.28 (m, 2H), 7.26 - 7.21 (m, 2H), 7.21 - 7.14 (m, 1H), 3.82 (d, J = 1.8 Hz, 1H), 3.78 (q, J = 6.7 Hz, 1H), 3.28 (s, 3H), 2.64 (s, 1H), 2.44 (d, J = 2.0 Hz, 1H), 1.63 (d, J = 11.1 Hz, 1H), 1.48 (m, 1H), 1.46 (d, J = 6.5 Hz, 3H), 1.08 (d, J = 11.1 Hz, 1H), 1.01 (t, J = 6.9 Hz, 1H), 0.54 (dt, J = 6.1, 3.0 Hz, 1H), 0.18 (q, J = 7.0 Hz, 1H). LCMS-ESI+ (m/z): [M+H]- calculated for C17H22NO2: 272.17; found: 272.1.

Step 2



[0114] A mixture of compound 8-B (3720 mg, 11.7 mmol) and 10% Pd/C (711 mg) in EtOH (60 mL) was stirred under H2 atmosphere. After 20 hours, the mixture was filtered through celite and the filtrate was concentrated, and the residue was used for the Boc protection. LCMS-ESI+ (m/z): [M+H]+ calculated for C9H14NO2: 168.10; found: 168.0.

[0115] The residue was stirred in THF (50 mL) at room temperature as Boc2O (6.00 g, 27.49 mmol) and DIEA (6 mL, 34.45 mmol) were added. After ∼30 min, the reaction mixture was concentrated to ∼1/3 volume, diluted with ethyl acetate, and washed with water (twice). After the aqueous fractions were extracted with ethyl acetate, the organic fractions were combined, dried (Na2SO4) and concentrated. The residue was purified by CombiFlash (120 g column) using hexanes - ethyl acetate as eluents to obtain compound 8-C. 1H NMR (400 MHz, Chloroform-d) δ 4.39 (s, 0.5H), 4.25 (s, 0.5H), 3.86 (s, 0.5H), 3.77 (s, 0.5H), 3.73 (s, 1.5H), 3.71 (s, 1.5H), 2.74 (m, 1H), 1.48 (s, 4.5H), 1.44 - 1.42 (m, 1H), 1.41 (s, 4.5H), 1.36 - 1.21 (m, 1H), 1.06 (m, 2H), 0.50 (dt, J = 5.9, 3.0 Hz, 1H), 0.27 (qd, J = 7.2, 2.8 Hz, 1H). LCMS-ESI+ (m/z): [M+H]+ calculated for C14H22NO4: 268.15; found: 267.7.

Step 3



[0116] A solution of compound 8-C (400 mg, 1.496 mmol) in THF (3 mL) was stirred at 0 °C as 2.0 M LiBH4 in THF (1.5 mL) was added. After 5 min, the mixture was stirred at room temperature. After 66 hours, the reaction mixture was diluted with ethyl acetate and added water slowly. After two phases were separated, the aqueous fraction was extracted with ethyl acetate and the two organic fractions were washed with water, combined, dried (Na2SO4), and concentrated. The residue was purified by CombiFlash (40 g column) using hexanes - ethyl acetate as eluents to yield compound 8-D. 1H NMR (400 MHz, Chloroform-d) δ 4.14 (dd, J = 2.3, 1.3 Hz, 1H), 3.68 - 3.53 (m, 2H), 3.50 - 3.41 (m, 1H), 2.61 (s, 1H), 2.39 (d, J = 2.1 Hz, 1H), 1.49 (s, 9H), 1.30 (td, J = 6.8, 6.2, 2.3 Hz, 1H), 1.16 (dt, J = 11.1, 1.8 Hz, 1H), 1.10 - 1.03 (m, 1H), 0.99 (td, J = 7.0, 3.0 Hz, 1H), 0.46 (dt, J = 6.5, 3.2 Hz, 1H), 0.22 (q, J = 7.1 Hz, 1H). LCMS-ESI+ (m/z): [M+H]+ calculated for C13H22NO3: 240.16; found: 239.7.

Step 4



[0117] A solution of compound 8-D (345 mg, 1.442 mmol), phthalimide (351 mg, 2.386 mmol), and PPh3 (852 mg, 3.248 mmol) in THF (20 mL) was stirred at 0 °C as DIAD (0.65 mL, 3.301 mmol) was added. After addition, the mixture was stirred at 0 °C for 30 min and then at rt. After 16 hours, the solution was concentrated to syrup and the residue was stirred in ether (50 mL) at 0 °C for 1.5 hours before filtration. The filtrate was concentrated, and the residue was purified using CombiFlash (40 g column) with hexane- ethyl acetate as eluents to obtain compound 8-E. 1H NMR (400 MHz, Chloroform-d) δ 7.84 (ddt, J = 10.3, 7.8, 3.8 Hz, 2H), 7.78 - 7.61 (m, 2H), 4.23 (s, 0.5H), 4.11 (m, 0.5H), 3.99 (dd, J = 13.1, 4.1 Hz, 0.5H), 3.88 (dd, J = 12.7, 6.7 Hz, 0.5H), 3.73 - 3.43 (m, 2H), 2.41 (d, J = 2.1 Hz, 1H), 1.49 (s, 4.5H), 1.49 - 1.2 (m, 2H), 1.31 (s, 4.5H), 1.09 (d, J = 11.5 Hz, 1H), 0.94 - 0.86 (m, 0.5H), 0.85 - 0.77 (m, 0.5H), 0.44 (m, 1H), 0.17 (m, 1H). LCMS-ESI+ (m/z): [M+H]+calculated for C21H25N2O4: 369.18; found: 368.9.

Step 5 and Step 6



[0118] To a solution of compound 8-E (516 mg, 1.401 mmol) in EtOH (30 mL) was added hydrazine hydrate (0.29 mL) at rt and the resulting solution was stirred at 70 °C. After 4.5 hours, the mixture was cooled to room temperature and diluted with ethyl ether (30 mL) and stirred at 0 °C for 30 min before filtration. The filtrate was concentrated and the residue was dissolved in CH2Cl2 before filtration to remove some insoluble material. The resulting filtrate was concentrated to obtain crude compound 8-F. LCMS-ESI+ (m/z): [M+H]+calculated for C13H23N2O2: 239.18; found: 238.9.

[0119] The mixture of crude compound 8-F, compound 8-G (341 mg, 1.408 mmol), and NaHCO3 (240 mg, 2.857 mmol) in water (4 mL) and EtOH (4 mL) was stirred at rt. After 15 hours, the mixture was diluted with water and extracted with ethyl acetate (twice). The extracts were washed with water, combined, dried (Na2SO4), concentrated. To the crude residue in CH2Cl2 (5 mL) was added 4 N HCl in dioxane (10 mL) at room temperature and the resulting mixture was stirred at room temperature for 2 hours. The mixture was concentrated, co-evaporated with toluene, and dried under vacuum for 30 min.

[0120] A suspension of the residue and DBU (1.06 mL, 7.088 mmol) in toluene (10 mL) was stirred at 110 °C bath. After 30 min, the mixture was concentrated and the residue was dissolved in CH2Cl2 (∼50 mL) and washed with aqueous NH4Cl (twice). After the aqueous fractions were extracted with CH2Cl2 (twice), the three organic fractions were combined, dried (Na2SO4), and concentrated. The residue was purified by CombiFlash (24 g column) using ethyl acetate - 20% MeOH/ethyl acetate as eluents to obtain compound 8-H. 1H NMR (400 MHz, Chloroform-d) δ 8.17 (s, 1H), 5.04 (s, 1H), 4.09 (s, 1H), 4.08 (s, 3H), 3.91 (s, 3H), 3.86 - 3.71 (m, 2H), 2.73 (d, J = 1.8 Hz, 1H), 1.43 - 1.21 (m, 2H), 1.13 (d, J = 12.1 Hz, 2H), 0.60 (dt, J = 6.7, 3.1 Hz, 1H), 0.40 (q, J = 7.3 Hz, 1H). LCMS-ESI+ (m/z): [M+H]+calculated for C17H19N2O5: 331.13; found: 331.2.

Step 7



[0121] A mixture of compound 8-H (40 mg, 0.121 mmol) in THF (1 mL) and MeOH (1 mL) was stirred at room temperature as 1 N KOH (1 mL) was added. After 30 min, the reaction mixture was acidified with 1 N HCl (∼1.1 mL), concentrated to ∼ 2 mL, and diluted with brine before extraction with CH2Cl2 (x 3). The combined extracts was dried (Na2SO4) and concentrated.

[0122] To the solution of crude acid were added 2,4-difluorobenzylamine (26 mg, 0.182 mmol), and HATU (56 mg, 0.147 mmol) at room temperature followed by DIEA (0.32 mL, 1.835 mmol). After 1 hour, additional 2,4-difluorobenzylamine (26 mg, 0.182 mmol) and HATU (56 mg, 0.147 mmol) were added. After 1 hour, the reaction mixture was diluted with water and the product was extracted with CH2Cl2 (x 2). The extracts were washed with water, combined, dried (Na2SO4) and concentrated.

[0123] The residue was purified by CombiFlash (24 g column) using ethyl acetate-20%MeOH/ethyl acetate as eluents to obtain compound 8-1. 1H NMR (400 MHz, Chloroform-d) δ 10.44 (t, J = 6.0 Hz, 1H), 8.36 (s, 1H), 7.34 (td, J = 8.6, 6.8 Hz, 1H), 6.87 - 6.69 (m, 2H), 5.02 (s, 1H), 4.60 (qd, J = 15.2, 5.9 Hz, 2H), 4.16 - 4.07 (m, 1H), 4.04 (s, 3H), 3.83 (t, J = 12.0 Hz, 1H), 3.76 (dd, J = 12.2, 2.7 Hz, 1H), 2.72 (d, J = 1.7 Hz, 1H), 1.39 - 1.21 (m, 2H), 1.18 - 1.07 (m, 2H), 0.59 (dt, J = 6.6, 3.2 Hz, 1H), 0.39 (q, J = 7.3 Hz, 1H). 19F NMR (376 MHz, Chloroform-d) δ -112.08 (p, J = 7.7 Hz), -114.77 (q, J = 8.6 Hz). LCMS-ESI+ (m/z): [M+H]+ calculated for C23H22F2N3O4: 442.16; found: 442.3.

Step 8



[0124] A suspension of compound 8-1 (47 mg, 0.106 mmol) in MeCN (2 mL) was stirred at 50 °C as MgBr2 (49 mg, 0.266 mmol) was added. After 30 min, the reaction mixture was stirred at 0 °C and added 1 N HCl to make the mixture a solution (∼2 mL). After the mixture was diluted with CH2Cl2 and water, two fractions were separated and the aqueous fraction was extracted with CH2Cl2 (twice). The combined organic fractions were dried (Na2SO4) and concentrated. The residue was purified by CombiFlash (24 g column) using CH2Cl2 and 20% MeOH in CH2Cl2 as eluents to obtain compound 8. The residue was triturated in MeCN at 0 °C for 30 min and filtered. The collected solids were dried in vacuum to obtain additional compound 8. 1H NMR (400 MHz, Chloroform-d) δ 11.68 (s, 1H), 10.43 (s, 1H), 8.28 (s, 1H), 7.36 (td, J = 8.6, 6.4 Hz, 1H), 6.86 - 6.75 (m, 2H), 4.96 (s, 1H), 4.64 (d, J = 6.0 Hz, 2H), 4.12 (d, J = 7.9 Hz, 1H), 3.81 (d, J = 7.6 Hz, 2H), 2.79 (d, J = 1.7 Hz, 1H), 1.42 (d, J = 11.0 Hz, 2H), 1.17 (d, J = 12.3 Hz, 2H), 0.65 (dt, J = 6.7, 3.2 Hz, 1H), 0.46 (q, J = 7.3 Hz, 1H). 19F NMR (376 MHz, Chloroform-d) δ -112.36 (p, J = 7.5 Hz), -114.76 (q, J = 8.6 Hz). LCMS-ESI+ (m/z): [M+H]+ calculated for C22H20F2N3O4: 428.14; found: 428.3.

Example 9


Preparation of Compound 9


(1aS,2S,10aS,11R,11aR)-5-hydroxy-4,6-dioxo-N-(2,4,6-trifluorobenzyl)-1a,2,4,6,10,10a,11,11a-octahydro-1H-2,11-methanocyclopropa[4,5]pyrido[1,2-a]pyrido[1,2-d]pyrazine-7-carboxamide



[0125] 


Step 1



[0126] A mixture of compound 8-H (84 mg, 0.254 mmol) in THF (2 mL) and MeOH (2 mL) was stirred at room temperature as 1 N KOH (1 mL) was added. After 30 min, the reaction mixture was concentrated to ∼2 mL, acidified with 1 N HCl (∼1.1 mL), concentrated to ∼2 mL, and diluted with brine before extraction with CH2Cl2 (thrice). The combined extracts was dried (Na2SO4) and the solution was used for the next reaction.

[0127] To the crude acid solution were added 2,4,6-trifluorobenzylamine (57 mg, 0.354 mmol), and HATU (157 mg, 0.413 mmol) at room temperature followed by DIEA (0.31 mL, 1.780 mmol). After ∼30 min, additional DIEA (0.31 mL, 1.78 mmol) was added. After 1 hour, the reaction mixture was washed with saturated NH4Cl and water. After the aqueous fractions were extracted with CH2Cl2, the two organic fractions were combined, dried (Na2SO4) and concentrated. The residue was purified by CombiFlash (24 g column) using ethyl acetate - 20%MeOH/ethyl acetate as eluents to obtain compound 9-A. 1H NMR (400 MHz, Chloroform-d) δ 10.37 (t, J = 5.7 Hz, 1H), 8.36 (s, 1H), 6.74 - 6.57 (m, 2H), 5.03 (s, 1H), 4.64 (qd, J = 14.5, 5.7 Hz, 2H), 4.11 - 4.06 (m, 1H), 4.04 (s, 3H), 3.83 (t, J = 12.0 Hz, 1H), 3.76 (dd, J = 12.2, 2.8 Hz, 1H), 2.74 (d, J = 1.8 Hz, 1H), 1.33 (dd, J = 13.7, 2.8 Hz, 2H), 1.19 - 1.08 (m, 2H), 0.60 (dt, J = 6.6, 3.1 Hz, 1H), 0.40 (q, J = 7.2 Hz, 1H). 19F NMR (376 MHz, Chloroform-d) δ -108.39 - - 109.90 (m, IF), -111.99 (t, J = 6.9 Hz, 2F). LCMS-ESI+ (m/z): [M+H]+ calculated for C23H21F3N3O4: 460.15; found: 460.3.

Step 2



[0128] A suspension of compound 9-A (106 mg, 0.231 mmol) in MeCN (4 mL) was stirred at 50 °C and MgBr2 (107 mg, 0.581 mmol) was added. After 30 min, the reaction mixture was stirred at 0 °C and 1 N HCl was added to obtain a solution (∼2 mL) After the mixture was diluted with CH2Cl2 and water, two fractions were separated and the aqueous fraction was extracted with CH2Cl2 (twice). The combined organic fractions were dried (Na2SO4) and concentrated. The residue was purified by CombiFlash (12 g column) using CH2Cl2 and 20% MeOH in CH2Cl2 as eluents to get 85 mg of compound 12. The residue was triturated in MeCN at 0 °C for 30 min and filtered. The collected solids were dried in vacuum to obtain compound 9. 1H NMR (400 MHz, Chloroform-d) δ 10.35 (s, 1H), 8.27 (s, 1H), 6.65 (dd, J = 8.7, 7.6 Hz, 2H), 4.96 (s, 1H), 4.66 (dd, J = 5.7, 4.0 Hz, 2H), 4.09 (d, J = 8.3 Hz, 1H), 3.80 (d, J = 8.1 Hz, 2H), 2.79 (s, 1H), 1.41 (d, J = 11.1 Hz, 2H), 1.18 (t, J = 10.6 Hz, 2H), 0.65 (dt, J = 6.8, 3.2 Hz, 1H), 0.46 (q, J = 7.3 Hz, 1H). 19F NMR (376 MHz, Chloroform-d) δ -109.13 - -109.32 (m, IF), -111.99 (t, J = 7.0 Hz, 2F). LCMS-ESI+ (m/z): [M+H]+ calculated for C22H19F3N3O4: 446.13; found: 446.3.

Example 10


Preparation of Compound 10


(1aS,2S,3aR,12R,12aR)-9-hydroxy-8,10-dioxo-N-(2,4,6-trifluorobenzyl)-1a,2,3a,4,8,10,12,12a-octahydro-1H-2,12-methanocyclopropa[e]pyrido[1',2':4,5]pyrazino[2,1-b][1,3]oxazepine-7-carboxamide



[0129] 


Step 1



[0130] A 100-mL 1-neck round bottom flask was charged with 7-G (0.26 g, 1.74 mmol), 10-A (0.8 g, 1.74 mmol), potassium carbonate (0.97 g, 7.03 mmol), acetic acid (2.55 g, 42.5 mmol) and acetonitrile (30 mL). The reaction mixture was stirred at 65 °C for 2 hours. After cooled back to room temperature, the reaction mixture was diluted with EtOAc (100 mL), washed with sat NaHCO3 and dried over Na2SO4. After concentration, the crude was purified by column chromatography on silica gel with hexane-EtOAc to obtain 10-B. [M+H]+ calculated for C21H20F2N3O5: 476.; found: 476.

Step 2



[0131] A 50-mL 1-neck round bottom flask was charged with 10-B (0.30 g, 0.63 mmol), magnesium bromide (0.30 g, 1.63 mmol) and acetonitrile (5 mL). The resulting mixture was stirred at 50 °C for 10 minutes. Then the mixture was stirred at 0 °C while 1 N HCl (∼4 mL) was added. Additional water (∼5 mL) was added to wash down solids forming on the flask walls. The resulting solid was filtrated and washed with water. After drying under high vacuum overnight, Compound 10 was obtained. 1H NMR (400 MHz, Chloroform-d) δ 12.31 (s, 1H), 10.32 (s, 1H), 8.31 (s, 1H), 6.83 - 6.54 (m, 2H), 5.90 (d, J = 9.2 Hz, 1H), 5.22 (d, J = 3.9 Hz, 1H), 4.79 - 4.47 (m, 4H), 4.22 (s, 1H), 4.08 - 3.86 (m, 1H), 1.92 - 1.64 (m, 2H), 1.65 - 1.43 (m, 2H), 0.86 (q, J = 7.4 Hz, 1H), 0.59 (dt, J = 6.6, 3.2 Hz, 1H). 19F NMR (376 MHz, Chloroform-d) δ -109.17 , -111.95 . [M+H]+ calculated for C21H20F2N3O5: 462; found: 462.

Example 11


Preparation of Compound 11


(1aR,2R,10aR,11S,11aS)-5-hydroxy-4,6-dioxo-N-(2,4,6-trifluorobenzyl)-1a,2,4,6,10,10a,11,11a-octahydro-1H-2,11-methanocyclopropa[4,5]pyrido[1,2-a]pyrido[1,2-d]pyrazine-7-carboxamide



[0132] 


Step 1



[0133] A suspension of compound 11-A (965 mg, 3.061 mmol), 2,4,6-trifluorobenzylamine (493 mg, 3.06 mmol), and HATU (1402 mg, 3.688 mmol) in CH2Cl2 (15 mL) was stirred in 0 °C as DIEA (2 mL, 11.48 mmol) was added.

[0134] After 1.5 hours at 0 °C, the reaction mixture was diluted with ethyl acetate, and washed with water (twice). After the aqueous fractions were extracted with ethyl acetate, the organic fractions were combined, dried (Na2SO4), and concentrated. The residue was purified by CombiFlash (40 g column) using hexanes-ethyl acetate as eluents to obtain compound 11-B. 1H NMR (400 MHz, Chloroform-d) δ 10.30 (t, J = 5.9 Hz, 1H), 8.40 (s, 1H), 6.79 - 6.51 (m, 2H), 4.65 (d, J = 5.6 Hz, 2H), 4.48 (t, J = 4.8 Hz, 1H), 4.01 (d, J = 4.8 Hz, 2H), 3.97 (s, 3H), 3.94 (s, 3H), 3.38 (s, 6H). 19F NMR (376 MHz, Chloroform-d) δ -109.07 - -109.35 (m, IF), -111.93 (t, J = 6.9 Hz, 2F). LCMS-ESI+ (m/z): [M+H]+ calculated for C20H22F3N2O7: 459.14; found: 459.2.

Step 2



[0135] A mixture of the compound 11-B (300 mg, 0.654 mmol) and methanesulfonic acid (63 mg, 0.655 mmol) in MeCN (3 mL) and acetic acid (0.3 mL) was heated to 75 °C for 2 hours. After cooling the solution, aminoalcohol 11-D (98 mg, 0.655 mmol), and K2CO3 (272 mg, 1.968 mmol) were added and the mixture was diluted with MeCN (10 mL) and stirred at 65 °C for 21 hours. The reaction mixture was concentrated to remove most of MeCN, diluted with water (∼30 mL) and extracted with ethyl acetate (∼30 mL, twice). The extracts were washed with water, combined, dried (Na2SO4), and concentrated. The residue was purified by CombiFlash (40 g column) using hexanes - ethyl acetate as eluents to obtain compound 11-E. 1H NMR (400 MHz, Chloroform-d) δ 10.27 (t, J = 5.7 Hz, 1H), 8.37 (s, 1H), 6.73 - 6.56 (m, 2H), 5.81 (dd, J = 9.9, 3.8 Hz, 1H), 5.25 (d, J = 3.9 Hz, 1H), 4.71 - 4.57 (m, 2H), 4.53 - 4.48 (m, 1H), 4.21 (dd, J = 12.8, 3.8 Hz, 1H), 4.02 (s, 3H), 3.98 (dd, J = 12.7, 9.9 Hz, 1H), 1.67 (dt, J = 13.5, 1.1 Hz, 2H), 1.54 (ddd, J = 7.5, 5.4, 3.6 Hz, 1H), 1.48 (dt, J = 13.5, 3.4 Hz, 1H), 0.79 (td, J = 8.0, 6.7 Hz, 1H), 0.54 (dt, J = 6.7, 3.4 Hz, 1H). 19F NMR (376 MHz, Chloroform-d) δ -108.89 - -109.13 (m, IF), -111.96 (t, J = 7.0 Hz, 2F). LCMS-ESI+ (m/z): [M+H]+ calculated for C23H21F3N3O5: 476.14; found: 476.3.

Step 3



[0136] A suspension of compound 11-E (151 mg, 0.318 mmol) in MeCN (4 mL) was stirred at 50 °C and MgBr2 (146 mg, 0.793 mmol) was added. After 30 min, the reaction mixture was stirred at 0 °C 1 N HCl and was added to obtain a solution (∼2 mL). The solution was diluted with water, and the product was extracted with CH2Cl2 (thrice). The combined organic extracts were dried (Na2SO4) and concentrated. The residue was purified by CombiFlash (24 g column) using CH2Cl2 and 20% MeOH in CH2Cl2 as eluents, then further purified by trituration in MeOH (∼2 mL). After the mixture was stored in the freezer, the solids were filtered and washed with MeOH. The collected solids were dried in vacuum to obtain compound 11. 1H NMR (400 MHz, Chloroform-d) δ 12.28 (s, 1H), 10.29 (t, J = 5.7 Hz, 1H), 8.27 (s, 1H), 6.72 - 6.58 (m, 2H), 5.89 (dd, J = 9.8, 4.1 Hz, 1H), 5.22 (d, J = 3.9 Hz, 1H), 4.73 - 4.59 (m, 2H), 4.59 - 4.54 (m, 1H), 4.19 (dd, J = 12.8, 4.1 Hz, 1H), 3.99 (ddd, J = 12.7, 9.9, 0.7 Hz, 1H), 1.79 - 1.74 (m, 1H), 1.72 (d, J = 13.5 Hz, 1H), 1.63 - 1.59 (m, 1H), 1.51 (dt, J = 13.6, 3.5 Hz, 1H), 0.90 - 0.81 (m, 1H), 0.59 (dt, J = 6.7, 3.4 Hz, 1H). 19F NMR (376 MHz, Chloroform-d) δ -109.21 (tt, J = 8.8, 6.3 Hz, IF), -111.98 (t, J = 6.9 Hz, 2F). LCMS-ESI+ (m/z): [M+H]+ calculated for C22H19F3N3O5: 462.13; found: 462.3.

Example 12


Preparation of Compound 12


(1aR,2R,10aR,11S,11aS)-5-hydroxy-4,6-dioxo-N-(2,4,6-trifluorobenzyl)-1a,2,4,6,10,10a,11,11a-octahydro-1H-2,11-methanocyclopropa[4,5]pyrido[1,2-a]pyrido[1,2-d]pyrazine-7-carboxamide



[0137] 




Step 1



[0138] 2,2-dihydroxyacetic acid (12-A) in MeOH was refluxed for 24h. After the reaction was cooled to room temperature and concentrated under vacuum, it was diluted with DCM (25 mL) and concentrated again to provide methyl 2-hydroxy-2-methoxyacetate (12-B).

[0139] 1H NMR (400 MHz, Chloroform-d) δ 4.86 (s, 1H), 3.82 (s, 3H), 3.48 (s, 3H).

Step 2



[0140] A solution of Compound 12-B in toluene was cooled to 0°C under N2. L(-)-alpha-Methylbenzylamine, 99+%, (99% ee) was added via syringe slowly. The reaction was warmed to room temperature and stirred for 1.5 hours. The presence of the starting material was monitored by thin layer chromatography (TLC).

[0141] The reaction was quenched with water and the aqueous and organic layers separated. The aqueous layer was extracted with EtOAc. The organic layers were combined and washed with brine, dried (Na2SO4), and concentrated to provide compound 12-C.

Step 3



[0142] A solution of compound 12-C in N, N-dimethylformamide was cooled to -15°C under N2. TFA was added via syringe slowly over 15 min. After stirring for 10 min, freshly cracked cyclopentadiene (6.76g, 0.102 mol) was added via syringe over 10 min. The reaction was stirred for 1.5 hours at -15 to -10°C and monitored via TLC and LCMS.

[0143] The reaction mixture was diluted with heptane (100mL), quenched with saturated aqueous Na2CO3, and stirred for 10 min. The layers were separated, the organic layer was washed with brine, dried (MgSO4), and concentrated. The crude mixture was purified from the organic layer by CombiFlash on silica gel with 0-50% EtOAc/Hexane to obtain compound 12-D.

[0144] 1H NMR (400 MHz, Chloroform-d) δ 7.32 - 7.11 (m, 5H), 6.42 (ddd, J = 5.6, 3.1, 1.3 Hz, 1H), 6.27 (dd, J = 5.6, 1.9 Hz, 1H), 4.31 (q, J = 1.6 Hz, 1H), 3.35 (s, 3H), 3.03 (q, J = 6.5 Hz, 1H), 2.93 - 2.88 (m, 1H), 2.22 (s, 1H), 1.42 (t, J = 5.8 Hz, 4H).

Step 4



[0145] The mixture of 12-D (1.77 g, 6.878 mmol) and Pd(OAc)2(31 mg, 0.138 mmol) in ether (30 mL) was stirred at 0°C as diazomethane in ether (freshly made) (10 mL) was added slowly. After the addition, the mixture was stirred for ∼30 min and TLC indicated a mixture of the starting material and the product. Additional diazomethane was added every 30 min until no starting material was detected via TLC. The reaction was quenched with AcOH (5mL) at 0°C and stirred for about 20 min, concentrated, and purified by Combiflash using silica gel column with Hexanes-EtOAc as eluent to obtain compound 12-E.

[0146] 1H NMR (400 MHz, Chloroform-d) δ 7.36 - 7.10 (m, 5H), 3.88 - 3.67 (m, 2H), 3.26 (s, 3H), 2.63 (s, 1H), 2.47 - 2.36 (m, 1H), 1.68 - 1.54 (m, 1H), 1.45 (d, J = 6.5 Hz, 4H), 1.13 - 0.94 (m, 2H), 0.54 (dt, J = 6.2, 3.1 Hz, 1H), 0.17 (q, J = 7.1 Hz, 1H).

Step 5



[0147] The mixture of compound 12-E (1 g, 3.7 mmol) and 10% Pd/C (1 g) in EtOH (150 mL) was stirred under H2 atmosphere for 36 hours. The reaction mixture was filtered through Celite and the filtrate was concentrated.

[0148] The residue obtained from the above hydrogenation was stirred in THF (20mL) at room temperature as Boc2O (1.7 g, 7.7 mmol) and DIPEA (2 mL, 11.6 mmol) were added and allowed to continue for one hour. The reaction mixture was concentrated, and the resulting residue was purified by CombiFlash on silica gel column using hexanes - EtOAc as eluents to obtain compound 12-F. LCMS: m/z=267.6.

[0149] LCMS-ESI+ (m/z): [M+H]+ calculated for Chemical Formula: C14H21NO4, Molecular Weight: 267.32; found: 267.67.

[0150] 1H NMR (400 MHz, Chloroform-d) δ 4.32 (dd, J = 55.7, 2.2 Hz, 1H), 3.82 (d, J = 43.3 Hz, 1H), 3.72 (d, J = 3.6 Hz, 3H), 2.78 - 2.68 (m, 1H), 1.52 - 1.36 (m, 11H), 1.29 (dq, J = 20.6, 7.0, 6.5 Hz, 1H), 1.13 - 0.97 (m, 1H), 0.50 (dt, J = 5.5, 3.0 Hz, 1H), 0.33 - 0.21 (m, 1H).

Step 6



[0151] Compound 12-F (850 mg, 3.18 mmol) in THF (6 mL) was stirred at 0°C as 2.0 M LiBH4 in THF (3.2 mL, 6.4 mmol) was added. After 5 min, the temperature was raised to room temperature and the reaction was allowed to proceed for 7 hours. The reaction was quenched with ice and diluted with EtOAc and saturated NH4Cl. The aqueous and organic phases were separated. The aqueous fraction was extracted with EtOAc and the two organic fractions were washed with water, combined, dried (Na2SO4), and concentrated. The crude product alcohol was used as is for the next step.

[0152] LCMS-ESI+ (m/z): [M+H]+ calculated for Chemical Formula: C13H21NO3
Molecular Weight: 239.31; found: 239.72.

[0153] A solution of the above alcohol (760mg, 3.18 mmol), phthalimide (701 mg, 4.77 mmol), and PPh3 (1.67 g, 6.36 mmol) in THF (20 mL) was stirred at 0°C as DIAD (1.3 mL, 6.36 mmol) was added. After addition, the mixture was stirred at 0°C for 30 min and then at room temperature overnight. The reaction was diluted with EtOAc and washed with saturated NH4Cl twice. After the aqueous and organic phases were separated, the aqueous fraction was extracted with EtOAcand the two organic fractions were combined, dried (Na2SO4), and concentrated. The crude product was purified using CombiFlash (silica gel column) with 0-50%EtOAc/Hexane as eluents to provide compound 12-G. 1H NMR indicated a mixture of two rotamers.

[0154] LCMS-ESI+ (m/z): [M+H]+ calculated for Chemical Formula: C21H24N2O4, Molecular Weight: 368.43; found: 368.81.

[0155] 1H NMR (400 MHz, Chloroform-d) δ 7.95 - 7.58 (m, 4H), 4.32 - 4.05 (m, 1H), 4.07 - 3.79 (m, 1H), 3.66 (s, 2H), 2.42 (d, J = 2.2 Hz, 1H), 1.65 - 1.15 (m, 11H), 1.10 (d, J = 11.5 Hz, 1H), 0.87 (d, J = 40.7 Hz, 1H), 0.45 (dt, J = 6.5, 3.2 Hz, 1H), 0.18 (q, J = 7.0 Hz, 1H).

Step 7



[0156] Hydrazine hydrate was added to a solution of the compound 12-G in EtOH at room temperature; the reaction was stirred at 75°C for ∼3 hours. The resulting mixture was cooled to room temperature and diluted with ethyl ether (30 mL) and stirred at 0°C for 60 min before filtration. The resulting filtrate was concentrated to provide compound 12-H. LCMS-ESI+ (m/z): [M+H]+ calculated for Chemical Formula: C13H22N2O2, Molecular Weight: 238.33 found: 238.87.

Step 8



[0157] A mixture of 12-H 2.85mmol), 12-1 (913mg, 2.87mmol), and NaHCO3 (482 mg, 5.74mmol) in water (10 mL) and EtOH (20 mL) was stirred at room temperature overnight. The mixture was diluted with brine and extracted with EtOAc (twice). The extracts were combined, dried (MgSO4), concentrated, and dried under vacuum for 30 min.

[0158] To a solution of the above crude reactant (1.6g) in CH2Cl2 (10 mL) was added 4 N HCl in dioxane (10 mL). The reaction was stirred for about 2 hours, concentrated to dryness, co-evaporated with toluene, and dried under vacuum for 30 min.

[0159] The mixture of the above crude reactant (2.87 mmol) and DBU (4.3 mL, 28.7mmol) in MeOH (30 mL) was stirred at 60°C bath for 120 min. The mixture was concentrated and the residue was purified by CombiFlash on silica gel using 0-20% MeOH/EtOAc as eluents to provide 12-J.

[0160] 1H NMR (400 MHz, Chloroform-d) δ 8.05 (s, 1H), 7.65 (s, 1H), 7.34 (s, 2H), 5.56 (d, J = 9.7 Hz, 1H), 5.14 (d, J = 9.9 Hz, 1H), 5.02 (s, 1H), 4.39 (d, J = 7.2 Hz, 2H), 3.96 (d, J = 11.6 Hz, 1H), 3.74 (d, J = 29.1 Hz, 1H), 2.68 (s, 1H), 2.04 (s, 1H), 1.56 (d, J = 4.6 Hz, 2H), 1.45 - 1.20 (m, 4H), 1.11 (d, J = 14.1 Hz, 2H), 0.58 (s, 1H), 0.38 (s, 1H).

[0161] LCMS-ESI+ (m/z): [M+H]+ calculated for Chemical Formula: C24H24N2O5, Molecular Weight: 420.46; found: 421.29.

Step 9



[0162] A mixture of 12-J (752 mg, 1.841 mmol) in THF (4 mL) and MeOH (4 mL) was stirred at room temperature as IN KOH (3.75 mL) was added. After 1 hour, the reaction mixture was acidified with 3N HCl (1mL), and diluted with brine before extraction with EtOAc. The combined extracts was dried (MgSO4) and concentrated.

[0163] To the mixture of the above crude reactant (158mg, 0.403 mmol) in DCM (4mL) were added 2,4,6-trifluorobenzyl amine (85 mg, 0.52 mmol), and HATU (230 mg, 0.604 mmol) at room temperature followed by DIPEA (0.3 mL, 1.6 mmol). After about 60 min, the reaction mixture was diluted with DCM, washed with saturated NaHCO3, dried (MgSO4), and concentrated. The residue was purified by CombiFlash on silica gel using 0-20%MeOH/EtOAc to provide compound 12-H.

Step 10



[0164] Compound 12-H (174mg, 0.325 mmol) was dissolved in TFA (2 mL) at room temperature and stirred for 30 min. The solution was concentrated and the residue was purified by CombiFlash (silica gel column) using 0-20% MeOH in CH2Cl2 to provide compound 12.

[0165] 1H NMR (400 MHz, Chloroform-d) δ 10.37 (t, J = 5.5 Hz, 1H), 8.29 (s, 1H), 6.65 (dd, J = 8.8, 7.4 Hz, 2H), 4.94 (s, 1H), 4.65 (d, J = 5.7 Hz, 2H), 4.13 (s, 1H), 3.81 (d, J = 5.2 Hz, 2H), 2.79 (s, 1H), 1.40 (d, J = 10.9 Hz, 2H), 1.18 (d, J = 12.5 Hz, 2H), 0.64 (dt, J = 6.7, 3.1 Hz, 1H), 0.45 (q, J = 7.3 Hz, 1H).

[0166] 19F NMR (376 MHz, cdcl3) δ -109.13 to -109.21 (1F), -111.98 to -112.02 (2F).

[0167] LCMS-ESI+ (m/z): [M+H]+ calculated for Chemical Formula: C22H18F3N304, Molecular Weight: 445.39.; found: 446.26.

Example 13


Preparation of Compound 13


(1aR,2R,10aR,11S,11aS)-5-hydroxy-4,6-dioxo-N-(2,4,5-trifluorobenzyl)-1a,2,4,6,10,10a,11,11a-octahydro-1H-2,11-methanocyclopropa[4,5]pyrido[1,2-a]pyrido[1,2-d]pyrazine-7-carboxamide



[0168] 


Step 1



[0169] A mixture of 12-J (752 mg, 1.841 mmol) in THF (4 mL) and MeOH (4 mL) was stirred at room temperature as IN KOH (3.75 mL) was added. After 1 hour, the reaction mixture was acidified with 3N HCl (1mL), and diluted with brine before extraction with EtOAc. The combined extracts was dried (MgSO4) and concentrated.

[0170] To the mixture of the above crude reactant (158mg, 0.403 mmol) in DCM (4mL) were added the benzyl amine (85 mg, 0.52 mmol), and HATU (230 mg, 0.604 mmol) at room temperature followed by DIPEA (0.3 mL, 1.6 mmol). After about 60 min, the reaction mixture was diluted with DCM, washed with saturated NaHCO3, dried (MgSO4), and concentrated. The residue was purified by CombiFlash on silica gel using 0-20%MeOH/EtOAc to provide compound 13-A.

Step 2



[0171] Compound 13-A (118mg, 0.22 mmol) was dissolved in TFA (2 mL) at room temperature and stirred for 30 min. The solution was concentrated and the residue was purified by CombiFlash (silica gel column) using 0-20% MeOH in CH2Cl2 to provide compound 13.

[0172] 1H NMR (400 MHz, Chloroform-d) δ 11.74 (s, 1H), 10.45 (s, 1H), 8.29 (s, 1H), 7.25 - 7.16 (m, 1H), 6.90 (td, J = 9.5, 6.4 Hz, 1H), 4.95 (s, 1H), 4.60 (d, J = 6.0 Hz, 2H), 4.23 - 4.05 (m, 1H), 3.84 (dd, J = 4.2, 2.4 Hz, 2H), 2.80 (d, J = 3.2 Hz, 2H), 1.42 (d, J = 10.9 Hz, 2H), 1.29 - 1.12 (m, 2H), 0.65 (dt, J = 6.8, 3.2 Hz, 1H), 0.46 (q, J = 7.3 Hz, 1H).

[0173] 19F NMR (376 MHz, cdcl3) δ -120.67, -136.05, -143.22 to -143.36.

[0174] LCMS-ESI+ (m/z): [M+H]+ calculated for Chemical Formula: C22H18F3N304, Molecular Weight: 445.39.; found: 446.29.

ANTIVIRAL ASSAY


Example 14


Antiviral Assays in MT4 Cells



[0175] For the antiviral assay utilizing MT4 cells, 0.4 µL of 189X test concentration of 3-fold serially diluted compound in DMSO was added to 40 µL of cell growth medium (RPMI 1640, 10% FBS, 1% penicillin/streptomycin, 1% L-Glutamine, 1% HEPES) in each well of 384-well assay plates (10 concentrations) in quidruplicate.

[0176] 1 mL aliquots of 2 × 106 MT4 cells are pre-infected for 1 and 3 hours respectively at 37 °C with 25 µL (MT4) or of either cell growth medium (mock-infected) or a fresh 1:250 dilution of an HIV-IIIb concentrated ABI stock (0.004 m.o.i. for MT4 cells). Infected and uninfected cells are diluted in cell growth medium and 35 µL of 2000 (for MT4) cells is added to each well of the assay plates.

[0177] Assay plates were then incubated in a 37 °C incubator. After 5 days of incubation, 25 µL of 2X concentrated CellTiter-Glo™ Reagent (catalog # G7573, Promega Biosciences, Inc., Madison, WI) was added to each well of the assay plate. Cell lysis was carried out by incubating at room temperature for 2-3 minutes, and then chemiluminescence was read using the Envision reader (PerkinElmer).

[0178] Compounds of the present invention demonstrate antiviral activity in this assay as depicted in Table 1 below. Accordingly, the compounds of the invention may be useful for treating the proliferation of the HIV virus, treating AIDS, or delaying the onset of AIDS or ARC symptoms.
Table 1
Compound NumbernM in MT-4
EC50CC50
1 9.6 14113
2 10.7 7804
3 9.9 4099
4 8.4 12829
5 1.6 50481
6 1.5 14062
7 2.7 4826
8 1.4 8843
9 1.4 10677
10 1.7 7587
11 1.5 10977
12 2.9 22792
13 2.3 7051


[0179] The data in Table 1 represent an average over time for each compound. For certain compounds, multiple assays have been conducted over the life of the project.


Claims

1. A pharmaceutical composition comprising a compound having the following Formula (I):

or a stereoisomer or pharmaceutically acceptable salt thereof, and
one or more additional therapeutic agents selected from the group consisting of GS-9131 and GS-9148,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.
 
2. A pharmaceutical composition of claim 1 wherein the compound of Formula I has the following Formula (Ia):

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.
 
3. A pharmaceutical composition of claim 1 wherein the compound of Formula I has the following Formula (Ib):

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.
 
4. A pharmaceutical composition of claim 1 wherein the compound of Formula I has the following Formula (Ic):

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.
 
5. A pharmaceutical composition of claim 1 wherein the compound of Formula I has the following Formula (Id):

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.
 
6. A pharmaceutical composition of claim 1 wherein the compound of Formula I has the following Formula (Ie):

or a stereoisomer or pharmaceutically acceptable salt thereof,
wherein:

Y1 and Y2 are each, independently, hydrogen, C1-3alkyl or C1-3haloalkyl;

R1 is phenyl substituted with one to three halogen atoms;

X is -O-, -NR2-, -CHR3- or a bond;

R2 and R3 are each, independently, hydrogen or C1-3alkyl; and

L is -C(Ra)2C(Ra)2-; and

each Ra is, independently, hydrogen, halo, hydroxyl, or C1-4alkyl, and

wherein two Ra groups on adjacent carbon atoms, together with the carbon atoms to which they are attached, form a carbocyclic ring having the following structure:

wherein each Rb is, independently, hydrogen or halo.
 
7. A pharmaceutical composition of any one of claims 1-6 wherein X is -O-, or -NH-, or -CH2-, or a bond..
 
8. A pharmaceutical composition of any one of claims 1-7 wherein

i) Y1 is C1-3alkyl and Y2 is hydrogen; or

ii) Y1 is hydrogen, methyl or CF3 and Y2 is hydrogen.


 
9. A pharmaceutical composition of any one of claims 1-5 wherein Y1 is C1-3 haloalkyl and Y2 is hydrogen.
 
10. A pharmaceutical composition of any one of claims 1-9 wherein

i) R1 is substituted with one halogen, optionally wherein R1 is 4-fluorophenyl or 2-fluorophenyl; or

ii) R1 is substituted with two halogens, optionally wherein R1 is 2,4-difluorophenyl, 2,3-difluorophenyl, 2,6-difluorophenyl, 3-fluoro-4-chlorophenyl, 3,4-difluorophenyl, 2-fluoro-4-chlorophenyl, or 3,5-difluorophenyl, optionally wherein R1 is 2,4-difluorophenyl.; or

iii) R1 is substituted with three halogens, optionally wherein R1 is 2,4,6-trifluorophenyl, 2,3,4-trifluorophenyl, or 2,4,5-trifluorophenyl, optionally wherein R1 is 2,4,6-trifluorophenyl or 2,3,4-trifluorophenyl, optionally wherein R1 is 2,4,6-trifluorophenyl.


 
11. A pharmaceutical composition of any one of claims 1-10 wherein each Rb is

i) independently hydrogen; or

ii) independently halogen, optionally wherein each Rb is fluoro.


 
12. A pharmaceutical composition of claim 1 wherein the compound of Formula I is:























or


 
13. A pharmaceutical composition of any one of claims 1-12 for use in a method of treating an HIV infection in a human having or at risk of having the infection, said method comprising administering to the human a pharmaceutical composition of any one of claims 1-12.
 
14. A pharmaceutical composition of any one of claims 1-12 for use in medical therapy or the prophylactic or therapeutic treatment of an HIV infection.
 


Ansprüche

1. Pharmazeutische Zusammensetzung umfassend eine Verbindung mit der folgenden Formel (I):

oder ein Stereoisomer oder pharmazeutisch verträgliches Salz davon,
und ein oder mehrere zusätzliche therapeutische Mittel ausgewählt aus der Gruppe bestehend aus GS-9131 und GS-9148,
wobei:

Y1 und Y2 jeweils unabhängig Wasserstoff, C1-3-Alkyl oder C1-3-Halogenalkyl sind;

R1 Phenyl, substituiert mit einem bis drei Halogenatomen, ist;

X -O-, -NR2-, -CHR3- oder eine Bindung ist;

R2 und R3 jeweils unabhängig Wasserstoff oder C1-3-Alkyl sind; und

L -C(Ra)2C(Ra)2- ist; und

jedes Ra unabhängig Wasserstoff, Halogen, Hydroxy oder C1-4-Alkyl ist, und

wobei zwei Ra-Gruppen an benachbarten Kohlenstoffatomen zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, einen carbocyclischen Ring mit der folgenden Struktur bilden:

wobei jedes Rb unabhängig Wasserstoff oder Halogen ist.
 
2. Pharmazeutische Zusammensetzung nach Anspruch 1, wobei die Verbindung der Formel I die folgende Formel (Ia) aufweist:

oder ein Stereoisomer oder pharmazeutisch verträgliches Salz davon,
wobei:

Y1 und Y2 jeweils unabhängig Wasserstoff, C1-3-Alkyl oder C1-3-Halogenalkyl sind;

R1 Phenyl, substituiert mit einem bis drei Halogenatomen, ist;

X -O-, -NR2-, -CHR3- oder eine Bindung ist;

R2 und R3 jeweils unabhängig Wasserstoff oder C1-3-Alkyl sind; und

L -C(Ra)2C(Ra)2- ist; und

jedes Ra unabhängig Wasserstoff, Halogen, Hydroxy oder C1-4-Alkyl ist, und

wobei zwei Ra-Gruppen an benachbarten Kohlenstoffatomen zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, einen carbocyclischen Ring mit der folgenden Struktur bilden:

wobei jedes Rb unabhängig Wasserstoff oder Halogen ist.
 
3. Pharmazeutische Zusammensetzung nach Anspruch 1, wobei die Verbindung der Formel I die folgende Formel (Ib) aufweist:

oder ein Stereoisomer oder pharmazeutisch verträgliches Salz davon,
wobei:

Y1 und Y2 jeweils unabhängig Wasserstoff, C1-3-Alkyl oder C1-3-Halogenalkyl sind;

R1 Phenyl, substituiert mit einem bis drei Halogenatomen, ist;

X -O-, -NR2-, -CHR3- oder eine Bindung ist;

R2 und R3 jeweils unabhängig Wasserstoff oder C1-3-Alkyl sind; und

L -C(Ra)2C(Ra)2- ist; und

jedes Ra unabhängig Wasserstoff, Halogen, Hydroxy oder C1-4-Alkyl ist, und

wobei zwei Ra-Gruppen an benachbarten Kohlenstoffatomen zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, einen carbocyclischen Ring mit der folgenden Struktur bilden:

wobei jedes Rb unabhängig Wasserstoff oder Halogen ist.
 
4. Pharmazeutische Zusammensetzung nach Anspruch 1, wobei die Verbindung der Formel I die folgende Formel (Ic) aufweist:

oder ein Stereoisomer oder pharmazeutisch verträgliches Salz davon,
wobei:

Y1 und Y2 jeweils unabhängig Wasserstoff, C1-3-Alkyl oder C1-3-Halogenalkyl sind;

R1 Phenyl, substituiert mit einem bis drei Halogenatomen, ist;

X -O-, -NR2-, -CHR3- oder eine Bindung ist;

R2 und R3 jeweils unabhängig Wasserstoff oder C1-3-Alkyl sind; und

L -C(Ra)2C(Ra)2- ist; und

jedes Ra unabhängig Wasserstoff, Halogen, Hydroxy oder C1-4-Alkyl ist, und

wobei zwei Ra-Gruppen an benachbarten Kohlenstoffatomen zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, einen carbocyclischen Ring mit der folgenden Struktur bilden:

wobei jedes Rb unabhängig Wasserstoff oder Halogen ist.
 
5. Pharmazeutische Zusammensetzung nach Anspruch 1, wobei die Verbindung der Formel I die folgende Formel (Id) aufweist:

oder ein Stereoisomer oder pharmazeutisch verträgliches Salz davon,
wobei:

Y1 und Y2 jeweils unabhängig Wasserstoff, C1-3-Alkyl oder C1-3-Halogenalkyl sind;

R1 Phenyl, substituiert mit einem bis drei Halogenatomen, ist;

X -O-, -NR2-, -CHR3- oder eine Bindung ist;

R2 und R3 jeweils unabhängig Wasserstoff oder C1-3-Alkyl sind; und

L -C(Ra)2C(Ra)2- ist; und

jedes Ra unabhängig Wasserstoff, Halogen, Hydroxy oder C1-4-Alkyl ist, und

wobei zwei Ra-Gruppen an benachbarten Kohlenstoffatomen zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, einen carbocyclischen Ring mit der folgenden Struktur bilden:

wobei jedes Rb unabhängig Wasserstoff oder Halogen ist.
 
6. Pharmazeutische Zusammensetzung nach Anspruch 1, wobei die Verbindung der Formel I die folgende Formel (Ie)aufweist:

oder ein Stereoisomer oder pharmazeutisch verträgliches Salz davon,
wobei:

Y1 und Y2 jeweils unabhängig Wasserstoff, C1-3-Alkyl oder C1-3-Halogenalkyl sind;

R1 Phenyl, substituiert mit einem bis drei Halogenatomen, ist;

X -O-, -NR2-, -CHR3- oder eine Bindung ist;

R2 und R3 jeweils unabhängig Wasserstoff oder C1-3-Alkyl sind; und

L -C(Ra)2C(Ra)2- ist; und

jedes Ra unabhängig Wasserstoff, Halogen, Hydroxy oder C1-4-Alkyl ist, und

wobei zwei Ra-Gruppen an benachbarten Kohlenstoffatomen zusammen mit den Kohlenstoffatomen, an die sie gebunden sind, einen carbocyclischen Ring mit der folgenden Struktur bilden:

wobei jedes Rb unabhängig Wasserstoff oder Halogen ist.
 
7. Pharmazeutische Zusammensetzung nach einem der Ansprüche 1-6, wobei X -O- oder -NH- oder -CH2- oder eine Bindung ist.
 
8. Pharmazeutische Zusammensetzung nach einem der Ansprüche 1-7, wobei

i) Y1 C1-3-Alkyl ist und Y2 Wasserstoff ist; oder

ii) Y1 Wasserstoff, Methyl oder CF3 ist und Y2 Wasserstoff ist.


 
9. Pharmazeutische Zusammensetzung nach einem der Ansprüche 1-5, wobei Y1 C1-3-Halogenalkyl ist und Y2 Wasserstoff ist.
 
10. Pharmazeutische Zusammensetzung nach einem der Ansprüche 1-9, wobei

i) R1 mit einem Halogen substituiert ist, gegebenenfalls wobei R1 4-Fluorphenyl oder 2-Fluorphenyl ist; oder

ii) R1 mit zwei Halogenen substituiert ist, gegebenenfalls wobei R1 2,4-Difluorphenyl, 2,3-Difluorphenyl, 2,6-Difluorphenyl, 3-Fluor-4-chlorphenyl, 3,4-Difluorphenyl, 2-Fluor-4-chlorphenyl oder 3,5-Difluorphenyl ist, gegebenenfalls wobei R1 2,4-Difluorphenyl ist; oder

iii) R1 mit drei Halogenen substituiert ist, gegebenenfalls wobei R1 2,4,6-Trifluorphenyl, 2,3,4-Trifluorphenyl oder 2,4,5-Trifluorphenyl ist, gegebenenfalls wobei R1 2,4,6-Trifluorphenyl oder 2,3,4-Trifluorphenyl ist, gegebenenfalls wobei R1 2,4,6-Trifluorphenyl ist.


 
11. Pharmazeutische Zusammensetzung nach einem der Ansprüche 1-10, wobei jedes Rb:

i) unabhängig Wasserstoff; oder

ii) unabhängig Halogen ist, gegebenenfalls wobei jedes Rb Fluor ist.


 
12. Pharmazeutische Zusammensetzung nach Anspruch 1, wobei die Verbindung der Formel I Folgende ist:























oder


 
13. Pharmazeutische Zusammensetzung nach einem der Ansprüche 1-12 für die Verwendung bei einem Verfahren zum Behandeln einer HIV-Infektion bei einem Menschen mit der Infektion oder mit einem Risiko, sie aufzuweisen, wobei das Verfahren Verabreichen einer pharmazeutischen Zusammensetzung nach einem der Ansprüche 1-12 an den Menschen umfasst.
 
14. Pharmazeutische Zusammensetzung nach einem der Ansprüche 1-12 für die Verwendung bei einer medizinischen Therapie oder bei der prophylaktischen oder therapeutischen Behandlung einer HIV-Infektion.
 


Revendications

1. Composition pharmaceutique comprenant un composé répondant à la formule (I) suivante :

ou stéréoisomère ou sel pharmaceutiquement acceptable de celle-ci, et
un ou plusieurs agents thérapeutiques supplémentaires choisis dans le groupe constitué par GS-9131 et GS-9148, dans laquelle :

Y1 et Y2 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ou halogénoalkyle en C1-3 ;

R1 est un groupe phényle substitué par un à trois atomes d'halogène ;

X est -O-, -NR2-, -CHR3- ou une liaison ;

R2 et R3 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ; et

L est -C(Ra)2C(Ra)2- ; et

chaque Ra est, indépendamment, l'atome d'hydrogène ou un groupe halogéno, hydroxyle ou alkyle en C1-4 et dans laquelle deux groupes Ra sur des atomes de carbone adjacents, conjointement avec les atomes de carbone auxquels ils sont attachés, forment un cycle carbocyclique ayant la structure suivante :

dans laquelle chaque Rb est, indépendamment, l'atome d'hydrogène ou un groupe halogéno.


 
2. Composition pharmaceutique selon la revendication 1 dans laquelle le composé de formule I répond à la formule (Ia) suivante :

ou stéréoisomère ou sel pharmaceutiquement acceptable de celle-ci,
dans laquelle :

Y1 et Y2 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ou halogénoalkyle en C1-3 ;

R1 est un groupe phényle substitué par un à trois atomes d'halogène ;

X est -O-, -NR2-, -CHR3- ou une liaison ;

R2 et R3 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ; et

L est -C(Ra)2C(Ra)2- ; et

chaque Ra est, indépendamment, l'atome d'hydrogène ou un groupe halogéno, hydroxyle ou alkyle en C1-4 et dans laquelle deux groupes Ra sur des atomes de carbone adjacents, conjointement avec les atomes de carbone auxquels ils sont attachés, forment un cycle carbocyclique ayant la structure suivante :

dans laquelle chaque Rb est, indépendamment, l'atome d'hydrogène ou un groupe halogéno.


 
3. Composition pharmaceutique selon la revendication 1 dans laquelle le composé de formule I répon à la formule (Ib) suivante :

ou stéréoisomère ou sel pharmaceutiquement acceptable de celle-ci,
dans laquelle :

Y1 et Y2 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ou halogénoalkyle en C1-3 ;

R1 est un groupe phényle substitué par un à trois atomes d'halogène ;

X est -O-, -NR2-, -CHR3- ou une liaison ;

R2 et R3 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ; et

L est -C(Ra)2C(Ra)2- ; et

chaque Ra est, indépendamment, l'atome d'hydrogène ou un groupe halogéno, hydroxyle ou alkyle en C1-4 et dans laquelle deux groupes Ra sur des atomes de carbone adjacents, conjointement avec les atomes de carbone auxquels ils sont attachés, forment un cycle carbocyclique ayant la structure suivante :

dans laquelle chaque Rb est, indépendamment, l'atome d'hydrogène ou un groupe halogéno.


 
4. Composition pharmaceutique selon la revendication 1 dans laquelle le composé de formule I répond à laformule (Ic) suivante :

ou stéréoisomère ou sel pharmaceutiquement acceptable de celle-ci,
dans laquelle :

Y1 et Y2 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ou halogénoalkyle en C1-3 ;

R1 est un groupe phényle substitué par un à trois atomes d'halogène ;

X est -O-, -NR2-, -CHR3- ou une liaison ;

R2 et R3 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ; et

L est -C(Ra)2C(Ra)2- ; et

chaque Ra est, indépendamment, l'atome d'hydrogène ou un groupe halogéno, hydroxyle ou alkyle en C1-4 et dans laquelle deux groupes Ra sur des atomes de carbone adjacents, conjointement avec les atomes de carbone auxquels ils sont attachés, forment un cycle carbocyclique ayant la structure suivante :

dans laquelle chaque Rb est, indépendamment, l'atome d'hydrogène ou un groupe halogéno.


 
5. Composition pharmaceutique selon la revendication 1 dans laquelle le composé de formule I répond à la formule (Id) suivante :

ou stéréoisomère ou sel pharmaceutiquement acceptable de celle-ci,
dans laquelle :

Y1 et Y2 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ou halogénoalkyle en C1-3 ;

R1 est un groupe phényle substitué par un à trois atomes d'halogène ;

X est -O-, -NR2-, -CHR3- ou une liaison ;

R2 et R3 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ; et

L est -C(Ra)2C(Ra)2- ; et

chaque Ra est, indépendamment, l'atome d'hydrogène ou un groupe halogéno, hydroxyle ou alkyle en C1-4 et dans laquelle deux groupes Ra sur des atomes de carbone adjacents, conjointement avec les atomes de carbone auxquels ils sont attachés, forment un cycle carbocyclique ayant la structure suivante :

dans laquelle chaque Rb est, indépendamment, l'atome d'hydrogène ou un groupe halogéno.


 
6. Composition pharmaceutique selon la revendication 1 dans laquelle le composé de formule I répond à la formule (Ie) suivante :

ou stéréoisomère ou sel pharmaceutiquement acceptable de celle-ci,
dans lequel :

Y1 et Y2 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ou halogénoalkyle en C1-3 ;

R1 est un groupe phényle substitué par un à trois atomes d'halogène ;

X est -O-, -NR2-, -CHR3- ou une liaison ;

R2 et R3 sont chacun, indépendamment, l'atome d'hydrogène ou un groupe alkyle en C1-3 ; et

L est -C(Ra)2C(Ra)2- ; et

chaque Ra est, indépendamment, l'atome d'hydrogène ou un groupe halogéno, hydroxyle ou alkyle en C1-4 et dans laquelle deux groupes Ra sur des atomes de carbone adjacents, conjointement avec les atomes de carbone auxquels ils sont attachés, forment un cycle carbocyclique ayant la structure suivante :

dans laquelle chaque Rb est, indépendamment, l'atome d'hydrogène ou un groupe halogéno.


 
7. Composition pharmaceutique selon l'une quelconque des revendications 1-6 dans laquelle X est -O- ou -NH- ou -CH2- ou une liaison.
 
8. Composition pharmaceutique selon l'une quelconque des revendications 1-7 dans laquelle

i) Y1 est un groupe alkyle en C1-3 et Y2 est l'atome d'hydrogène; ou

ii) Y1 est l'atome d'hydrogène, un groupe méthyle ou CF3 et Y2 est l'atome d'hydrogène.


 
9. Composition pharmaceutique selon l'une quelconque des revendications 1-5 dans laquelle Y1 est un groupe halogénoalkyle en C1-3 et Y2 est l'atome d'hydrogène.
 
10. Composition pharmaceutique selon l'une quelconque des revendications 1-9 dans laquelle

i) R1 est substitué par un atome d'halogène, éventuellement dans laquelle R1 est un groupe 4-fluorophényle ou 2-fluorophényle ; ou

ii) R1 est substitué par deux atomes d'halogène, éventuellement R1 est un groupe 2,4-difluorophényle, 2,3-difluorophényle, 2,6-difluorophényle, 3-fluoro-4-chlorophényle, 3,4-difluorophényle, 2-fluoro-4-chlorophényle ou 3,5-difluorophényle, éventuellement dans lequel R1 est un groupe 2,4-difluorophényle ; ou

iii) R1 est substitué par trois atomes d'halogène, éventuellement R1 est un groupe 2,4,6-trifluorophényle, 2,3,4-trifluorophényle ou 2,4,5-trifuorophényle, éventuellement R1 est un groupe 2,4,6-trifluoro dans lequel R1 est un groupe 2,4,6-trifluorophényle.


 
11. Composition pharmaceutique selon l'une quelconque des revendications 1-10 dans laquelle chaque Rb est

i) indépendamment l'atome d'hydrogène ; ou

ii) indépendamment un atome d'halogène, éventuellement chaque Rb étant un groupe fluoro.


 
12. Composition pharmaceutique selon la revendication 1 dans laquelle le composé de formule I est :























ou


 
13. Composition pharmaceutique selon l'une quelconque des revendications 1-12, destinée à être utilisée dans un procédé de traitement d'une infection par le VIH chez un être humain ayant l'infection ou présentant un risque d'avoir l'infection, ledit procédé comprenant l'administration à l'être humain d'une composition pharmaceutique selon l'une quelconque des revendications 1-12.
 
14. Composition pharmaceutique selon l'une quelconque des revendications 1-12 destinée à être utilisée en thérapie médicale ou dans le traitement prophylactique ou thérapeutique d'une infection par le VIH.
 






Cited references

REFERENCES CITED IN THE DESCRIPTION



This list of references cited by the applicant is for the reader's convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard.

Patent documents cited in the description




Non-patent literature cited in the description