(19)
(11)EP 3 461 896 A3

(12)EUROPEAN PATENT APPLICATION

(88)Date of publication A3:
26.06.2019 Bulletin 2019/26

(43)Date of publication A2:
03.04.2019 Bulletin 2019/14

(21)Application number: 18191841.8

(22)Date of filing:  12.07.2012
(51)International Patent Classification (IPC): 
C12N 15/66(2006.01)
C12N 15/31(2006.01)
C07K 19/00(2006.01)
C12N 1/00(2006.01)
C40B 40/06(2006.01)
C12N 15/62(2006.01)
C12Q 1/68(2018.01)
C07K 14/24(2006.01)
C12N 5/00(2006.01)
(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30)Priority: 15.07.2011 US 201161508366 P
21.02.2012 US 201261601409 P
13.03.2012 US 201261610212 P

(62)Application number of the earlier application in accordance with Art. 76 EPC:
12814750.1 / 2732038

(71)Applicant: The General Hospital Corporation
Boston, MA 02114 (US)

(72)Inventors:
  • JOUNG, J., keith
    Winchester, MA 01890 (US)
  • SANDER, Jeffry, D.
    Ames, IA 50010 (US)

(74)Representative: Donald, Jenny Susan 
Forresters IP LLP Skygarden Erika-Mann-Strasse 11
80636 München
80636 München (DE)

  


(54)METHODS OF TRANSCRIPTION ACTIVATOR LIKE EFFECTOR ASSEMBLY


(57) The disclosure describes methods that include providing a first nucleic acid having a sequence encoding a first set comprising one or more transcription activator-like effector (TALE) repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the first nucleic acid with a first enzyme, wherein the first enzyme creates a first ligatable end; providing a second nucleic acid having a sequence encoding a second set comprising one or more TALE repeat domains and/or one or more portions of one or more TALE repeat domains; contacting the second nucleic acid with a second enzyme, wherein the second enzyme creates a second ligatable end, and wherein the first and second ligatable ends are compatible; and ligating the first and second nucleic acids through the first and second ligatable ends to produce a first ligated nucleic acid, wherein the first ligated nucleic acid is linked to a solid support, and wherein the first ligated nucleic acid encodes a polypeptide comprising said first and second sets.





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