(19)
(11)EP 3 501 497 A1

(12)EUROPEAN PATENT APPLICATION

(43)Date of publication:
26.06.2019 Bulletin 2019/26

(21)Application number: 18214199.4

(22)Date of filing:  19.12.2018
(51)International Patent Classification (IPC): 
A61K 9/00(2006.01)
A61K 31/192(2006.01)
A61K 47/34(2017.01)
A61K 31/7056(2006.01)
A61K 47/10(2017.01)
A61K 9/06(2006.01)
A61K 47/14(2017.01)
A61P 17/10(2006.01)
(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR
Designated Extension States:
BA ME
Designated Validation States:
KH MA MD TN

(30)Priority: 21.12.2017 CN 201711392438

(71)Applicant: Zhaoke (Guangzhou) Ophthalmic Drug Company Limited
230088 Guangzhou Guangdong (CN)

(72)Inventors:
  • Liu, Jing
    Hefei City, Anhui 230080 (CN)
  • Li, Gang
    Hefei City, Anhui 230088 (CN)
  • Li, Xiaoyi
    Shatin (HK)
  • Dai, Xiangrong
    Hefei City, Anhui 230088 (CN)
  • Yin, Lei
    Hefei City, Anhui 230088 (CN)
  • Ling, Juan
    Hefei City, Anhui 230088 (CN)

(74)Representative: Isern Patentes y Marcas S.L. 
Avda. Diagonal, 463 Bis, 2°
08036 Barcelona
08036 Barcelona (ES)

  


(54)DISPERSION PROCESS OF ADAPALENE IN A GEL PREPARATION


(57) The present invention relates to the field of medicine, and specifically discloses a dispersion process of adapalene gel preparation, which comprises the following steps: pulverizing adapalene raw material to D50 not more than 10µm and D90 not more than 30µm by dry detection; adding methyl p-hydroxybenzoate, 1,2-propanediol, carbomer 980 and disodium edetate in water, heating and stirring consistently to obtain a matrix in a uniform jelly; adding poloxamer 188, propylene glycol and ethylene glycol phenyl ether in water, stirring and heating to prepare a mixed solution; adding adapalene in the mixed solution prepared, emulsifying at a high speed, then adding to the matrix for thorough stirring; and then adding a triethanolamine aqueous solution for homogenization and stirring. The preparation prepared by the process of the invention has good emulsifying and dispersing effect of adapalene, can be expanded on a large scale, and the industrial promotion prospect is good.


Description

FIELD



[0001] The invention relates to the field of medicine, in particular to a dispersion process of adapalene in a gel preparation.

BACKGROUND



[0002] Adalalene is a white or off-white powder chemical, with a chemical name 6-[3-(1-adamantyl)-4-methoxyphenyl]-2-naphthoic acid, molecular formula C28H28O3, molecular weight 412.52000, which is insoluble in water or ethanol, slightly soluble in tetrahydrofuran. Adapalene is a dermatological drug that is clinically suitable for the treatment of acne vulgaris, which is mainly characterized by acne, papules and pustules. It can also be used to treat acne on the face, chest and back.

[0003] Adapalene mainly binds to RARβ and RARγ, and has weak binding to RARα. It inhibits glutamine invertase of keratinocytes in vitro, inhibits the keratinization process, and regulates cell differentiation. Animal experiments have found that adapalene has acne dissolution and local anti-inflammatory effects, and its anti-inflammatory activity may be related to its interference with the function of polymorphonuclear leukocytes and the metabolism of arachidonic acid.

[0004] Adapalene is poorly soluble, so adapalene is present in suspension in aqueous gels. For suspension gels, the pharmacopoeia has strict requirements. The Appendix 0114 Gel Particle Size in 2015 edition of the Pharmacopoeia requires: no particles more than 180µm should be detected for the suspension gel. Therefore, the size of adapalene drug substance plays a crucial role in the absorption of this product. The particle size relates to the homogeneity of the formulation, bioavailability, formulation stability, and the like. None of the relevant patents and literatures that have been published places studies in detail on the particle size of adapalene. For a gelling preparation, the emulsification and dispersion process is also very important, which directly determines the quality of the preparation. Current patent documents are as follows:

CN201510809500.4, titled "a method for preparing adapalene gel"

CN201310393546.3 titled "an adapalene gel and a preparation method thereof"

CN201310350418.0 titled "an adapalene gel and a preparation method thereof"

CN201210378524.5 titled "adapalene gel"

CN200510135286.5 titled "an adapalene gel composition and a preparation method thereof"



[0005] These patents specify that the formulation has a particle size of less than 50µm, but does not systematically study the particle size of adapalene, crushing process and the detailed particle size distribution. Moreover, the disclosed dispersion processes of the above patent documents are all stirring process in small-scale, the batch size is small, and all the preparations are emulsified and dispersed after the formulation is finished. Because of large volume of the preparation which needs emulsification and dispersion, the emulsifying and dispersing effect is not good, resulting in poor uniformity and poor appearance of the preparation, thus, the uniformity and stability of the preparation can not be guaranteed under the particle size. Because the preparation which needs to be emulsified and dispersed is bulky, the emulsification and dispersion effect is not good, resulting in poor uniformity and poor appearance of the preparation, thereby the uniformity and stability of the preparation produced under such particle size cannot be guaranteed.

SUMMARY



[0006] It is an object of the present invention to provide a dispersion process for a gel formulation of adapalene comprising the following steps:

Step 1: pulverizing adapalin raw material until D50 under dry detection not more than 10um and D90 under dry detection not more than 30um;

Step 2: adding methyl p-hydroxybenzoate, 1,2-propanediol, carbomer 980, disodium edetate in water, heating and stirring persistently to obtain a matrix in a uniform jelly;

Step 3: adding poloxamer 188, propylene glycol, ethylene glycol phenyl ether in water, stirring and heating to prepare a mixed solution;

Step 4: adding adapalene to the mixed solution prepared in step 3; after high-speed emulsification adding to the matrix and stirring well; then adding triethanolamine aqueous solution to homogenize and stir.



[0007] Preferably, in step 1, the adapalene starting material is pulverized using a jet mill. More preferably, the crushing parameters are a venturi-tube pressure of 8.0-11.0 bar, an annular pressure of 8.0-10.0 bar, and a feed rate of 50-150 rpm.

[0008] Preferably, the heating in steps 2 and 3 means heating to 50-60 °C.

[0009] Preferably, the mass ratio of water, methylparaben, 1,2-propanediol, carbomer 980, and disodium edetate in step 2 is 480 : 2-4 : 80-100 : 5- 7 : 1-2.

[0010] Preferably, the mass ratio of water to poloxamer 188, propylene glycol, ethylene glycol phenyl ether in step 3 is 1:30-40:1.

[0011] Preferably, the mass ratio of adapalene to the mixed solution in step 4 is 1:20-200.

[0012] More preferably, the emulsification in step 4 is emulsified at a speed of 12-20 m/s for 20-40 min.

[0013] Preferably, the method of the invention further comprises a sterilization treatment step.

[0014] More preferably, the sterilization treatment is moist heat sterilization at 121 °C.

[0015] The inventor of the present invention conducted a comprehensive scientific study on the particle size control and emulsification dispersion process of adapalene, and solved the problem of emulsification dispersion and reasonable particle size control of adapalene gel preparation. In specific embodiments, the test result of the adapalene compound gel prepared by the dispersion process of the invention shows that the particle size requirement is met: D90≤50um; the content uniformity requirement: China Pharmacopoeia 2015 stipulating that A+2.2S<15. The emulsification and dispersing effect of adapalene in the preparation prepared by the process of the invention is good, and the process can be expanded on a large scale, and the industrial promotion prospect is good.

DETAILED DESCRIPTION



[0016] The present invention discloses a dispersion process of adapalene in a gel preparation, and those skilled in the art can learn from the contents herein and appropriately improve the process parameters. It is to be understood that all such alternatives and modifications are apparent to those skilled in the art and are considered to be included in the present invention. The processes of the present invention have been described in terms of the preferred embodiments, and it is apparent that those skilled in the art can change and adapt and combine the methods and applications described herein to implement and apply the present invention without departing from the contents, spirits and scope of the invention.

[0017] In order to make those skilled in the art better understand the technical solutions of the present invention, the present invention will be further described in detail below with reference to specific embodiments.

Example 1: Dispersion process of adapalene


1. pulverization and particle size control of adapalene raw material


(1) pulverization of adapalene raw materials



[0018] Method: The adapalene was pulverized using a jet mill to achieve the target particle size. Pulverization parameters: venturi-tube pressure 8.0 bar, annular pressure 8.0 bar, feed rate 50 rpm

(2) Particle size control:



[0019] Method: Taking the appropriate amount of this product, using Malvern MS3000 laser particle size analyzer, dry detection, D50 should not greater than 10µm, D90 should not greater than 30um.

2. Dispersing process of adapalene


(1) Matrix preparation



[0020] 60Kg purified water was added to the emulsifying tank and heated to 55 ±5 °C. Homogenization and stirring was started, then 250g methyl parahydroxybenzoate and 10kg 1, 2-propanediol were added and stirred homogeneously for 10 min, then 700g carbomer 980, 125g ethylenediamine tetraacetic acid disodium were added and stirred homogeneously for 20 minutes. A uniform gelatinized matrix was obtained.

(2) Solution preparation



[0021] 

1) 5Kg purified water was added into the mixing tank, stirring was started and 1.125Kg triethanolamine was added;

2) 5Kg purified water was added to the tank, stirring was started, and 200g Poloxamer 188 was added to the tank, heating to 55 ±5 °C was started, stirred for 15 minutes, and 6 kg 1,2 propanediol and 200 g ethylene glycol phenyl ether were added and stirred for 5 min.

(3) Sterilization



[0022] The marix, triethanolamine aqueous solution, Poloxamol 188 aqueous solution, 1,2-propanediol and ethylene glycol phenyl ether mixture were sterilized under moisture heating at 121 °C for 20 minutes.

(4) Preparation process



[0023] 
  1. 1) Clindamycin hydrochloride was dissolved in 7Kg purified water to prepare clindamycin hydrochloride solution, after aseptic filtration, the solution was added directly to the matrix and mixed well.
  2. 2) 125g adapalene was added to a sterilized mixture solution of 2.5Kg 1, 2-propanediol, ethylene glycol phenyl ether and Poloxamer 188 mixture, high speed emulsification (speed 15m/s) and dispersion was carried out for 30 mins in the emulsifying tank, then added into the matrix to stir, then triethanolamine aqueous solution was added slowly to the emulsifying tank, and was stirred homogeneously for 20 minutes.
  3. 3) Bubbles were removed in vacuum. Samples were taken and the viscosity, pH, the content of clindamycin hydrochloride, and the content and uniformity of adapalene were measured.

Example 2:


1. pulverization and particle size control of adapalene raw material


(1) pulverization of adapalene raw materials



[0024] Method: The adapalene was pulverized using a jet mill to achieve the target particle size. Pulverization parameters: venturi-tube pressure 9.0 bar, annular pressure 9.0 bar, feed rate 100 rpm

(2) Particle size control:



[0025] Method: Taking the appropriate amount of this product, using Malvern MS3000 laser particle size analyzer, dry detection, D50 should not greater than 10µm, D90 should not greater than 30um.

3. Dispersing process of adapalene


(1) Matrix preparation



[0026] 60Kg purified water was added to the emulsifying tank and heated to 55 ±5 °C. Homogenization and stirring was started, then 500g methyl parahydroxybenzoate and 12kg 1,2-propanediol were added and stirred homogeneously for 10 min, then 750g carbomer 980, 200g ethylenediamine tetraacetic acid disodium were added and stirred homogeneously for 20 minutes. A uniform gelatinized matrix was obtained.

(2) Solution preparation



[0027] 

1) 5.5Kg purified water was added into the mixing tank, stirring was started and 1.125Kg triethanolamine was added;

2) 5Kg purified water was added to the tank, stirring was started, and 500g Poloxamer 188 was added to the tank, heating to 55 ±5 °C was started, stirred for 15 minutes, and 15 kg 1,2 propanediol and 500 g ethylene glycol phenyl ether were added and stirred for 5 min.

(3) Sterilization



[0028] The marix, triethanolamine aqueous solution, Poloxamol 188 aqueous solution, 1,2-propanediol and ethylene glycol phenyl ether mixture were sterilized under moisture heating at 121 °C for 20 minutes.

(4) Preparation process



[0029] 
  1. 1) Clindamycin hydrochloride was dissolved in 7Kg purified water to prepare clindamycin hydrochloride solution, after aseptic filtration, the solution was added directly to the matrix and mixed well.
  2. 2) 125g adapalene was added to a sterilized mixture solution of 5Kg 1,2-propanediol, ethylene glycol phenyl ether and Poloxamer 188 mixture, high speed emulsification (speed 15m/s) and dispersion was carried out for 30 mins in the emulsifying tank, then added into the matrix to stir, then triethanolamine aqueous solution was added slowly to the emulsifying tank, and was stirred homogeneously for 20 minutes.
  3. 3) Bubbles were removed in vacuum. Samples were taken and the viscosity, pH, the content of clindamycin hydrochloride, and the content and uniformity of adapalene were measured.

Example 3:



[0030] 

(1) The pulverization: After pulverization the raw materials of adapalene was subjected to dry method detection and D50 was found not more than 10um, D90 was found not more than 30um. Adapalene was pulverized using a jet mill to achieve the target particle size.
Pulverization parameters: Venturi-tube pressure 10.0 bar, annular pressure 9.0 bar, feed speed 100rpm.

(2) Matrix preparation:
Methyl parahydroxybenzoate and 1,2-propanediol were added into purified water, stirred evenly and heated continuously. Carbomer 980 and disodium ethylenediamine tetraacetic acid were added and stirred to form a uniform gelatinized matrix;
Specifically, 60Kg purified water was added to the emulsifying tank and heated to 55 ±5°C. After homogenization and stirring were started, 250g methyl parahydroxybenzoate and 10kg 1,2-propanediol were added, then 700g carbomer 980 and 125g ethylenediamine tetraacetic acid disodium was added, stirred homogeneously for 20 minutes, to form a uniform gelatinized matrix.

(3) Solution preparation: Poloxamer 188, propanediol and ethylene glycol phenyl ether were added into purified water, stirred and heated for 5 min. 5Kg purified water was added to the tank, stirring was started, and 500g Poloxamer 188 was added, heating was started to 55 ±5°C, stirred for 15 minutes, 15 kg 1,2-propanediol and 200 g ethylene glycol phenyl ether were added and stirred.

(4) Dispersion steps:
Adapalene was added to a mixture solution of 1,2-propanediol, eethylene glycol phenyl ether and Poloxamer 188, after high speed emulsification, added the matrix to stir fully; then triethanolamine aqueous solution was added and stirred homogeneously. In the emulsifying tank, high speed emulsification (15m/s) (12∼20m/s) was performed to disperse for 30min (20∼40min), then added the matrix and stirred evenly, triethanolamine aqueous solution was slowly added into the emulsifying tank, and stirred homogeneously for 20 minutes.
Clindamycin hydrochloride solution can be optionally added to prepare clindamycin hydrochloride adapalene compound gel.

5) Bubbles were removed in vacuum. Samples were taken and the viscosity, pH, the content of clindamycin hydrochloride, and the content and uniformity of adapalene were measured.


Example 4:



[0031] The results of adapalene hydrochloride clindamycin compound gel prepared from examples 1-3 are as follows:

1. Uniformity of the preparation



[0032] 
Batch numberpHviscosity (mP•s)particle size (D90)clindamycin hydrochloride content (%)adapalene content uniformity (%)
20150511 6.82 5680 17.5um 99.79 101.20% A+2.2S=2.11
20150513 6.84 5570 16.7um 98.76 103.91% A+2.2S=7.69
20150515 6.88 5870 17.0um 102.86 102.73% A+2.2S=6.11
Note: particle size requirements: D90≤50um;


[0033] Content uniformity requirement: According to China Pharmacopoeia 2015, A +2.2S < 15 is in accordance with the provisions.
detection method of particle size of the preparation:
5-10g of the product was taken and mixed with 25ml water. The saturated solution of adalalene in Tween-80 was used as the dispersing medium, the rotational speed was 1500rpm, and the ultrasonic intensity was 20%.

2. Stability of the preparation



[0034] 
batch no.time (months)appearancepH valueviscosity (mP•s)total impurity in adapalene (%)total impurity in clindamycin (%)adalalene content (%)clindamycin content (%)
20150511 0 milky white gel 6.82 5680 0.34 1.18 101.20 99.79
3 milky white gel 6.84 5650 0.40 1.36 99.83 99.22
6 milky white gel 6.98 5612 0.43 1.60 99.35 98.78
9 milky white gel 6.82 5633 0.35 1.80 99.68 98.58
12 milky white gel 6.74 5590 0.38 2.02 99.70 98.41
18 milky white gel 6.80 5566 0.40 2.21 99.47 98.18
24 milky white gel 6.88 5525 0.44 2.46 99.23 98.30
20150513 0 milky white gel 6.84 5570 0.32 1.28 103.91 98.76
3 milky white gel 6.84 5618 0.38 1.38 103.22 98.65
6 milky white gel 6.80 5590 0.40 1.64 102.98 98.50
9 milky white gel 6.78 5532 0.38 1.82 102.90 98.54
12 milky white gel 6.79 5450 0.44 2.10 102.98 98.32
18 milky white gel 6.83 5440 0.42 2.21 102.87 98.04
24 milky white gel 6.88 5323 0.42 2.40 102.89 97.77
20150515 0 milky white gel 6.88 5870 0.35 1.22 102.73 102.86
3 milky white gel 6.80 5830 0.40 1.34 102.44 102.54
6 milky white gel 6.83 5805 0.41 1.55 102.56 102.20
9 milky white gel 6.80 5780 0.38 1.75 102.54 101.90
12 milky white gel 6.84 5800 0.44 1.98 102.21 101.56
18 milky white gel 6.80 5735 0.41 2.22 102.45 101.53
24 milky white gel 6.85 5700 0.40 2.39 102.2 101.22


[0035] According to the above data, it can be known that the uniformity and stability of the prepared preparations 20150511, 20150513 and 20150515 are good. Compared with 0-month sample, no significant change was found for the long-term 24-month sample with respect to all test indicators.

3. Pharmacokinetics and clinical study of the preparation



[0036] Efficacy and safety assessments were performed through human pharmacokinetics studies and clinical studies. The results of clinical trials show that the safety of the product is good, and it has remarkable curative effect on acne vulgaris.

[0037] The above description only shows preferred embodiment of the present invention, and it should be noted that those skilled in the art can also make a number of improvements and modifications without departing from the principles of the present invention, and these improvements and modifications should be considered falling within the scope of protection of the present invention.


Claims

1. A dispersion process of adapalene gel formulation, characterized by comprising the steps of:

step 1: pulverizing adapalene raw material to D50 not more than 10µm and D90 not more than 30µm by dry detection;

step 2: adding methyl p-hydroxybenzoate, 1,2-propanediol, carbomer 980 and disodium edetate in water, heating and stirring consistently to obtain a matrix in a uniform jelly;

step 3: adding poloxamer 188, propylene glycol and ethylene glycol phenyl ether in water, stirring and heating to prepare a mixed solution;

step 4: adding adapalene in the mixed solution prepared in step 3, emulsifying at a high speed, then adding to the matrix for thorough stirring; and then adding a triethanolamine aqueous solution for homogenization and stirring.


 
2. The dispersion process according to claim 1, characterized in that in step 1, a jet mill is used to pulverize adapalene starting material.
 
3. The dispersion process according to claim 2, characterized in that the pulverization parameters are venturi-tube pressure 8.0-11.0 bar, annular pressure 8.0-10.0 bar, and feed rate 50-150 rpm.
 
4. The dispersion process according to claim 1, characterized in that the heating in steps 2 and 3 is heated to 50-60 °C.
 
5. The dispersion process according to claim 1, characterized in that the mass ratio of the water, methylparaben, 1,2-propanediol, carbomer 980, and disodium edetate in step 2 is 480:2-4:80-100:5-7:1-2.
 
6. The dispersion process according to claim 1, characterized in that the mass ratio of water and poloxamer 188, propylene glycol, and ethylene glycol phenyl ether in step 3 is 1:30-40:1.
 
7. The dispersion process according to claim 1, characterized in that the mass ratio of adapalene to the mixed solution of step 4 is 1:20-200.
 
8. The dispersion process according to claim 1, characterized in that the emulsification in step 4 is performed at a speed of 12-20 m/s for 20-40 min.
 
9. The dispersion process according to claim 1, characterized in that further comprising a sterilization step.
 
10. The dispersion process according to claim 9, characterized in that the sterilization is a moist heat sterilization at 121 °C.
 





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Cited references

REFERENCES CITED IN THE DESCRIPTION



This list of references cited by the applicant is for the reader's convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard.

Patent documents cited in the description




Non-patent literature cited in the description