(19)
(11)EP 3 530 342 B1

(12)EUROPEAN PATENT SPECIFICATION

(45)Mention of the grant of the patent:
29.04.2020 Bulletin 2020/18

(21)Application number: 17862471.4

(22)Date of filing:  15.03.2017
(51)International Patent Classification (IPC): 
B01D 57/02(2006.01)
C12N 7/02(2006.01)
B03C 5/02(2006.01)
B03C 5/00(2006.01)
B01L 3/00(2006.01)
C12N 7/00(2006.01)
(86)International application number:
PCT/JP2017/010339
(87)International publication number:
WO 2018/073991 (26.04.2018 Gazette  2018/17)

(54)

CONCENTRATING DEVICE SUITABLE FOR DIELECTROPHORESIS AND METHOD FOR CONCENTRATION OF PARTICLES USING SAME

KONZENTRATIONSVORRICHTUNG FÜR DIELEKTROPHORESE UND VERFAHREN ZUR KONZENTRATION VON PARTIKELN DAMIT

DISPOSITIF DE CONCENTRATION APPROPRIÉ POUR LA DIÉLECTROPHORÈSE ET PROCÉDÉ DE CONCENTRATION DE PARTICULES L'UTILISANT


(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30)Priority: 21.10.2016 JP 2016206610

(43)Date of publication of application:
28.08.2019 Bulletin 2019/35

(73)Proprietor: Panasonic Intellectual Property Management Co., Ltd.
Osaka-shi, Osaka 540-6207 (JP)

(72)Inventors:
  • SHIMBA, Noriko
    Osaka-shi, Osaka 540-6207 (JP)
  • NISHIO, Kazuaki
    Osaka-shi, Osaka 540-6207 (JP)

(74)Representative: Grünecker Patent- und Rechtsanwälte PartG mbB 
Leopoldstraße 4
80802 München
80802 München (DE)


(56)References cited: : 
WO-A1-2013/069122
JP-A- 2010 187 664
US-A1- 2007 152 206
JP-A- 2005 506 191
US-A1- 2005 040 044
US-A1- 2014 246 321
  
  • CHIHIRO ASAI et al.: "29a-G17-11 Influence of flow rate and pillar alignment on the collection volume of bacteria in dielectrophoretic device with micro pillar array", 60th JSAP Spring Meeting, Kanagawa, 27-30 March 2013, vol. 60, 11 March 2013 (2013-03-11), page 12-195, XP009513483,
  
Note: Within nine months from the publication of the mention of the grant of the European patent, any person may give notice to the European Patent Office of opposition to the European patent granted. Notice of opposition shall be filed in a written reasoned statement. It shall not be deemed to have been filed until the opposition fee has been paid. (Art. 99(1) European Patent Convention).


Description

BACKGROUND


1. Technical Field



[0001] The present invention relates to a concentration device suitable for dielectrophoresis and a method for concentrating particles with the same.

2. Description of the Related Art



[0002] FIG. 25 is a duplicate of FIG. 1 included in Patent Literature 1. Patent Literature 1 discloses a dielectrophoresis system of handling dielectric particles, particularly biological cells, which are suspended in a medium and subjected to the action of an alternating electrical field. The distribution of said field is made non-uniform using a regular network (R) of electrodes (E1, E2) which can define local areas (L) where the electrical field is minimum in order to concentrate particles in said local zones (L) as a result of the action of the negative dielectrophoresis forces. The inventive system is characterised in that the network (R) of electrodes (E1, E2) is formed on the surface of a multi-layered support (1). Moreover, said system is characterised in that the electrodes (E1, E2) in the network (R) having the same polarity are linked to a common power supply contact (P1, P2) via two networks (R1, R2) of strip conductors (C1, C2) which are formed at an intermediary level located below the network (R) of electrodes.

[0003] Patent Literature 2 relates to a system for using dielectrophoresis to manipulate dielectric particles, in particular biological cells in suspension in a medium and subjected to the action of an alternating electric field of distribution that is made to be non-uniform by means of a regular array of electrodes suitable for defining local zones where the electric field is at a minimum to concentrate particles in said local zones by the action of negative dielectrophoresis forces, wherein the array of electrodes is formed on the surface of a multilayer substrate, and wherein the same-polarity electrodes of the array are connected to respective common power supply pads via two arrays of conductor tracks which are formed at an intermediate level situated beneath the array of electrodes.

[0004] Patent Literature 3 discloses a three-dimensional dielectrophoretic device which includes a substrate with electrodes having a predetermined gap spacing; a member formed on the substrate to forming a flow channel for a micro-dielectric introduced thereinto; and a plurality of projected members erected midway of the flow channel on the substrate. In this device, by disproportionating an electric field generated between the electrodes by the projected members, dielectrophoretic force is generated by the micro-dielectric flown in via the flow channel.

[0005] Patent Literature 4 relates to a particle separation device comprising a particle inflow section that draws in a suspension containing a plurality of dielectric particles suspended therein; a particle separating section including a flow channel to which the particle suspension is supplied from the particle inflow section and into which the particle suspension flows, a plurality of micropillars each formed from an electrical insulating material and disposed in the flow channel, and a voltage source that generates an electric field in the flow channel having the micropillars disposed therein; the plurality of micropillars are placed in such a position that the micropillars form electrically sparse and dense regions of the electric field generated by the voltage source to deflect the particles in a definite direction in the suspension; a particle concentrated liquid outflow channel that draws out a particle concentrated liquid containing concentrated particles separated by the particle separating section; and a particle-free liquid outflow channel that draws out a particle-free liquid from which the particles have been removed by the particle separating section.

[0006] Patent Literature 5 discloses a device and method for manipulating particles using dielectrophoresis. The device comprises a chamber comprising an inlet port, an outlet port, and metal post electrodes, and a power supply, wherein the metal post electrodes are arranged in at least two rows in a vertical position with respect to the flow of fluids, each row comprises at least two metal post electrodes, each odd row is wired to a metal pad through a metal line, and each even row is wired to another metal pad through a metal line, and the power supply is connected to the metal pads.

CITATION LIST


[Patent Literature]



[0007] 

[Patent Literature 1] JP 2005/506191 A

[Patent Literature 2] US 2005/040044 A1

[Patent Literature 3] JP 2010/187664 A

[Patent Literature 4] US 2014/246321 A1

[Patent Literature 5] US 2007/152206 A1


SUMMARY



[0008] An object of the present invention is to provide a concentration device suitable for dielectrophoresis and a method for concentrating particles with the same.

[0009] The present invention provides a concentration device suitable for dielectrophoresis, comprising:

a first substrate;

a second substrate provided so as to face the first substrate;

a flow path formed between the first substrate and the second substrate;

a first pillar electrode line disposed in the flow path; and

a second pillar electrode line disposed in the flow path,

wherein

the first pillar electrode line and the second pillar electrode line are parallel to an X-axis direction;

the first pillar electrode line and the second pillar electrode line include first pillar electrodes and second pillar electrodes;

each of the first pillar electrodes includes a first vertex P1 and a second vertex P2;

each of the second pillar electrode includes a first vertex Q1 and a second vertex Q2;

a line segment between the first vertex P1 and the second vertex P2 which are included in each of the first pillar electrodes is parallel to the X-axis direction;

a line segment between the first vertex Q1 and the second vertex Q2 which are included in each of the second pillar electrodes is parallel to a Y-axis direction;

the X-axis direction is perpendicular to the Y-axis direction in a top view;

a pillar electrode group is composed of

a left-side first pillar electrode L selected from the first pillar electrodes included in the second pillar electrode line;

a right-side first pillar electrode R selected from the first pillar electrodes included in the second pillar electrode line;

a second pillar electrode A selected from the second pillar electrodes included in the first pillar electrode line; and

a second pillar electrode B selected from the second pillar electrodes included in the second pillar electrode line;

the left-side first pillar electrode L and the right--side first pillar electrode R are adjacent to each other in a top view in such a manner that the second vertex P2 of the left-side first pillar electrode L and the first vertex P1 of the right-side first pillar electrode R face each other;

a line which passes through the second vertex Q2 of the second pillar electrode A and the first vertex Q1 of the second pillar electrode B is parallel to the Y-axis direction; and

the following mathematical formula (I) is satisfied:

where

A1 represents a distance between the second vertex Q2 of the second pillar electrode A and a center point O;

A2 represents a distance between the first vertex Q1 of the second pillar electrode B and the center point O;

the center point O is an intersection point of a line segment P and a line segment Q,

the line segment Q is a line segment between the second vertex Q2 of the second pillar electrode A and the first vertex Q1 of the second pillar electrode B; and

the line segment P is a line segment between the second vertex P2 of the left-side first pillar electrode L and the first vertex P1 of the right-side first pillar electrode R.



[0010] The present invention provides a concentration device suitable for dielectrophoresis and a method for concentrating particles with the same.

BRIEF DESCRIPTION OF THE DRAWINGS



[0011] 

FIG. 1A shows a top view of a concentration device according to the embodiment.

FIG. 1B shows a cross-sectional view taken along the line 1B-1B included in FIG. 1A.

FIG. 1C shows a cross-sectional view taken along the line 1C-1C included in FIG. 1A.

FIG. 1D shows a schematic view of a back surface of a first substrate 110.

FIG. 2 shows a top view of first pillar electrodes 301 and second pillar electrodes 302.

FIG. 3 shows a top view of two adjacent first pillar electrodes 301 and one second pillar electrode 302 thereby.

FIG. 4 shows a top view of the two first pillar electrodes 301 and the two second pillar electrodes 302.

FIG. 5 is a fluorescent microscope photograph of a flow path 103 through which a sample solution flows without applying an alternating voltage in the inventive example 1.

FIG. 6 is a fluorescent microscope photograph of the flow path 103 through which the sample solution flows while an alternating voltage is applied in the inventive example 1.

FIG. 7 is a fluorescent microscope photograph of the flow path 103 after five seconds of an end of the application of the alternating voltage in the inventive example 1.

FIG. 8 is a fluorescent microscope photograph of the flow path 103 through which a sample solution flows without applying an alternating voltage in the inventive example 2.

FIG. 9 is a fluorescent microscope photograph of the flow path 103 through which the sample solution flows while an alternating voltage is applied in the inventive example 2.

FIG. 10 is a fluorescent microscope photograph of the flow path 103 after five seconds of an end of the application of the alternating voltage in the inventive example 2.

FIG. 11 is a fluorescent microscope photograph taken before an application of an alternating voltage in the inventive example 3.

FIG. 12 is a fluorescent microscope photograph taken during an application of the alternating voltage in the inventive example 3.

FIG. 13 is a top view of the simulation result in the simulation example A1.

FIG. 14 is a top view of the simulation result in the simulation example A2.

FIG. 15 is a top view of the simulation result in the simulation example A3.

FIG. 16 shows a top view of the two first pillar electrodes 301 and the two second pillar electrodes 302.

FIG. 17 is a graph showing the simulation results in the example B1.

FIG. 18 is a graph showing the simulation results in the example B2.

FIG. 19 is a graph showing the simulation results in the example B3.

FIG. 20 is a graph showing the simulation results in the example B4.

FIG. 21 is a graph showing the simulation results in the example B5.

FIG. 22 is a graph showing the simulation results in the example B6.

FIG. 23 is a graph showing the simulation results in the example B7.

FIG. 24A shows a cross-sectional view of a silicon substrate 90 in one step included in a method for fabricating a concentration device according to the embodiment.

FIG. 24B shows a cross-sectional view of the silicon substrate 90 in one step, subsequent to FIG. 24A, included in the method.

FIG. 24C shows a cross-sectional view of the silicon substrate 90 in one step, subsequent to FIG. 24B, included in the method.

FIG. 24D shows a cross-sectional view of the silicon substrate 90 in one step, subsequent to FIG. 24C, included in the method.

FIG. 24E shows a cross-sectional view of the silicon substrate 90 in one step, subsequent to FIG. 24D, included in the method.

FIG. 24F shows a cross-sectional view of the silicon substrate 90 in one step, subsequent to FIG. 24E, included in the method.

FIG. 25 is a duplicate of FIG. 1 included in Patent Literature 1.


DETAILED DESCRIPTION OF THE EMBODIMENT



[0012] Hereinafter, the embodiment the present invention will be described in more detail with reference to the drawings. FIG. 1A shows a top view of a concentration device according to the embodiment. FIG. 1B shows a cross-sectional view taken along the line 1B-1B included in FIG. 1A. FIG. 1C shows a cross-sectional view taken along the line 1C-1C included in FIG. 1A.

[0013] As shown in FIG. 1B and FIG. 1C, the concentration device according to the embodiment comprises a first substrate 110 and a second substrate 105. The second substrate 105 faces the first substrate 110. A flow path 103 is formed between the first substrate 110 and the second substrate 105. The flow path 103 has a height H and a width W.

[0014] FIG. 1D shows a schematic view of the back surface of the first substrate 110. FIG. 1A is a schematic view of the front surface of the first substrate 110. As shown in FIG. 1A- FIG. 1D, the first substrate 110 comprises an inlet 101 and an outlet 102. The inlet 101 and the outlet 102 are through-holes. The inlet 101 communicates with the outlet 102 through the flow path 103. Therefore, a samples solution is injected through the inlet 101. The thus-injected samples solution flows through the flow path 103. Finally, the samples solution is discharged from the outlet 102. As is clear from FIG. 1A - FIG. 1D, the concentration device according to the present embodiment is a chip for concentration (hereinafter, which may be referred to as a "concentration chip").

[0015] The back surface of the first substrate 110 is provided with a first comb-shaped electrode 201 and a second comb-shaped electrode 202. The first comb-shaped electrode 201 and the second comb-shaped electrode 202 engage each other.

[0016] The inside of the flow path 103 is provided with first pillar electrodes 301 and second pillar electrodes 302. The first pillar electrodes 301 are electrically connected to the first comb-shaped electrode 201. The second pillar electrodes 302 are electrically connected to the second comb-shaped electrode 202. Of course, the first pillar electrodes 301 are electrically insulated from the second comb-shaped electrode 202. Similarly, the second pillar electrodes 302 are electrically insulated from the first comb-shaped electrode 201.

[0017] Now, the first pillar electrodes 301 and the second pillar electrodes 302 will be described in more detail. FIG. 2 shows a top view of the first pillar electrodes 301 and the second pillar electrodes 302. When viewed in the top view, each of the first pillar electrodes 301 has a shape of a rugby ball. Each of the second pillar electrodes 302 also has a shape of a rugby ball. One pillar electrode line 303 is composed of plural first pillar electrodes 301 and plural second pillar electrodes 302. In other words, one pillar electrode line 303 includes plural first pillar electrodes 301 and plural second pillar electrodes 302. Plural pillar electrode lines 303 are formed in the flow path 103. Each of the pillar electrode lines 303 is parallel to an X-axis direction. In this way, two or more pillar electrode lines 303 are formed in the flow path 103.

[0018] FIG. 3 shows a top view of two adjacent first pillar electrodes 301 and one second pillar electrode 302 thereby. Each of the first pillar electrodes 301 includes a first vertex P1 and a second vertex P2. The line segment between the first vertex P1 and the second vertex P2 included in each of the first pillar electrodes 301 has a length L1 in the X-axis direction. Each of the second pillar electrodes 302 includes a first vertex Q1 and a second vertex Q2. The line segment between the first vertex Q1 and the second vertex Q2 included in each of the second pillar electrodes 302 has a length L2 in the Y-axis direction. The first vertex Q1 is near the first vertex P1 and the second vertex P2. On the other hand, the second vertex Q2 is far from the first vertex P1 and the second vertex P2. In other words, as shown in FIG. 2, the second vertex Q2 included in the second pillar electrode 302 included in the first pillar electrode line 303a is closer to the second pillar electrode line 302b than the first vertex Q1 included in the second pillar electrodes 302 included in the first pillar electrode line 303a. A third pillar electrode line 303c may be provided. Needless to say, the X-axis direction is perpendicular to the Y-axis direction.

[0019] FIG. 4 shows an enlarged view of a region U surrounded by a dot line included in FIG. 2. FIG. 4 is a drawing showing one pillar electrode group included in the region U. As shown in FIG. 4, the one pillar electrode group is composed of the following four electrodes (I) - (IV):
  1. (I) one left-side first pillar electrode 301L selected from the first pillar electrodes 301 included in the second pillar electrode line 303b;
  2. (II) one right-side first pillar electrode 301R selected from the first pillar electrodes 301 included in the second pillar electrode line 303b;
  3. (III) one second pillar electrode 302A selected from the second pillar electrodes 302 included in the first pillar electrode line 303a; and
  4. (IV) one second pillar electrode 302B selected from the second pillar electrodes 302 included in the second pillar electrode line 303b.


[0020] The left-side first pillar electrode 301 L and the right-side first pillar electrode 301 R are adjacent to each other in such a manner that the second vertex P2 of the left-side first pillar electrode 301 L and the first vertex P1 of the right-side first pillar electrode 301 R face each other.

[0021] As is clear from FIG. 4, a line which passes through the second vertex P2 of the left-side first pillar electrode 301 L and the first vertex P1 of the right-side first pillar electrode 301 R is parallel to the X-axis direction.

[0022] A line which passes through the second vertex Q2 of the second pillar electrode A and the first vertex Q1 of the second pillar electrode B is parallel to the Y-axis direction.

[0023] As shown in FIG. 4, the center point O, the line segment P, and the line segment Q are defined as below.

[0024] The center point O is an intersection point of the line segment P and the line segment Q. The four electrodes included in the one pillar electrode group surround the center point O in the top view.

[0025] The line segment Q is a line segment between the second vertex Q2 of the second pillar electrode 302A and the first vertex Q1 of the second pillar electrode 302B.

[0026] The line segment P is a line segment between the second vertex P2 of the left-side first pillar electrode 301L and the first vertex P1 of the right-side first pillar electrode 301 R.

[0027] The present embodiment is characterized by that the following mathematical formula (I) is satisfied.

where

A1 represents a distance between the second vertex Q2 of the second pillar electrode 302A and the center point O; and

A2 represents a distance between the first vertex Q1 of the second pillar electrode 302B and the center point O.



[0028] In the present embodiment, since the value of L3 is not less than 5 micrometers, as demonstrated in the simulation examples B1 - B7, dielectrophoresis force is given as attractive force to particles flowing through the center point O. For this reason, the particles are captured only at and around the center point O against the stream of the fluid (i.e., the sample solution) flowing through the flow path 103. In this way, the particles are concentrated at and around the center point O. In case where the value of L3 is 0 micrometers, as demonstrated in the simulation examples B1 - B7, dielectrophoresis force is given as repulsive force to the particles flowing through the center point O. For this reason, the repulsive force accelerates the stream of the particles flowing through the flow path 103. Consequently, the particles fail to be captured. The particles flow faster toward the lower course of the flow path 103.

[0029] As demonstrated in the simulation examples B1 - B4, it is desirable that the value of L3 is not less than 5 micrometers. See FIG. 17 - FIG. 20.

[0030] As demonstrated in the simulation example B5, it is desirable that the value of A2 is not more than 2.8 micrometers. See FIG. 21.

[0031] As demonstrated in the simulation example B6, it is desirable that the length of the line segment P is not more than 5.6 micrometers. See FIG. 22.

(Fabrication method)



[0032] Hereinafter, a method for fabricating the concentration devices according to the embodiment will be described with reference to FIG. 24A- FIG. 24F. The concentration device according to the embodiment may be fabricated using a common fabrication method of semiconductor devices.

[0033] First, as shown in FIG. 24A, a first resist 901 is patterned on an upper surface of a silicon substrate 90 comprising an insulating layer 91 at the bottom surface thereof. Then, as shown in FIG. 24B, a second resist 902 is further patterned. Subsequently, as shown in FIG. 24C, a part of the silicon substrate 90 on which neither the first resist 901 nor the second resist 902 is formed is etched using both of the first resist 901 and the second resist 902 as a mask. A bottom of the etched part is located in the inside of the silicon substrate 90.

[0034] As shown in FIG. 24D, the second resist 902 is removed. Then, as shown in FIG. 24E, the silicon substrate 90 is etched using the first resist 901 as a mask. The bottom of the etched part reaches a front surface of the insulating layer 91. At this stage, the flow path 103 having the height H and the width W is formed. Finally, the first resist 901 is removed as shown in FIG. 24F. In this way, the first substrate 110 is formed. After the inlet 101 and the outlet 102 are formed, the first substrate 110 is joined onto the second substrate 105.

(Concentration method)



[0035] Hereinafter, a method for concentrating particles contained in a sample solution using a concentration device according to the embodiment will be described. In the present embodiment, each of the particles has a diameter of not less than 30 nanometers and not more than 100 nanometers. An example of the particle is influenza virus (particle size: approximately 100 nanometers) or norovirus (particle size: approximately 30 nanometers).

[0036] First, the concentration device according to the embodiment is prepared. Specifically, a user of the concentration device purchases the concentration device according to the embodiment from the present patentee or its licensee.

[0037] Then, the sample solution is supplied between the first substrate 110 and the second substrate 105. Specifically, the sample solution is injected through the inlet 101. The injected sample solution flows through the flow path 103.

[0038] While the sample solution flows through the flow path 103, an alternating voltage is applied between the first pillar electrodes 301 and the second pillar electrodes 302 through the first comb-shaped electrode 201 and the second comb-shaped electrode 202. Desirably, the applied alternating voltage has a voltage of not less than 5 volts pp and not more than 20 volts pp and a frequency of not less than 50 kilohertz and not more than 20 megahertz. The term "pp" means peak-to-peak.

[0039] This alternating voltage forms a region having a significantly high electric field at and around the center point O. This high electric field gives dielectrophoresis force as the attractive force at and around the center point O. Due to this dielectrophoresis force as the attractive force, the particles are captured only at and around the center point O against the stream of the fluid (namely, the sample solution) flowing through the flow path 103. In other words, the dielectrophoresis force as attractive force is greater than force given to the particles by the sample solution flowing through the flow path 103 along the +Y direction. In this way, the particles are concentrated center only at and around the center point O. Finally, the sample solution is discharged from the outlet 102, whereas the particles are left at and around the center point O.

[0040] As above described, the value of L3 is not less than 5 micrometers. In case where the value of L3 is 0 micrometers, as demonstrated in the simulation examples B1 - B7, dielectrophoresis as the repulsive force given to the particles accelerates the stream of the particles. For this reason, the particles are not captured and flow faster toward the lower course of the flow path (namely, in the +Y direction).

[0041] The word "parallel" used in the instant specification may include an angular error of not more than 5 degrees. Likewise, the word "perpendicular" may include an angular error of not more than 5 degrees.

EXAMPLES



[0042] Hereinafter, the present invention will be described in more detail with reference to the following examples.

(Inventive example 1)



[0043] In the inventive example 1, a sample solution containing fluorescence polystyrene particles was used. The sample solution was prepared by diluting fluorescence polystyrene particles (available from Polysciences company, trade name: Fluoresbrite Yellow Green Carboxylate Microspheres, particle size: 0.1 micrometer, w/v concentration: 2.6%) 100,000 fold with a 1% Tween20 aqueous solution. The sample solution had fluorescence polystyrene particle concentration of approximately 4.7x108 ml-1.

[0044] A SOI substrate was etched as shown in FIG. 24A- FIG. 24F to fabricate a first substrate 110. The first substrate 110 was joined onto a second substrate 105. In this way, a concentration device according to the inventive example 1 was fabricated.

[0045] The following table 1 shows the details of the concentration device according to the inventive example 1.
[Table 1]
Number of Pillar Electrode Lines 303 26
Number of First Pillar Electrodes 301 in each pillar electrode line 303 26
Number of Second Pillar Electrodes 302 in each pillar electrode line 303 35
L1 25.5 micrometers
L2 25.5 micrometers
P 2.8 micrometers
Q 31.1 micrometers
A1 29.7 micrometers
A2 1.4 micrometers
L3 28.3 micrometers
H 50 micrometers
W 1,000 micrometers


[0046] Then, ethanol was supplied at a flow rate of 20 microliters/minute through an inlet 101 for five minutes. In this way, air was removed from the flow path 103. Furthermore, 1 % Tween20 aqueous solution was supplied at a flow rate of 20 microliter/minute through the inlet 101 for five minutes. In this way, the ethanol was removed from the flow path 103. The flow path 103 was filled with 1 % Tween20 aqueous solution.

[0047] Then, a sample solution was supplied at a flow rate of 20 microliter/minute through the inlet 101 for five minutes. FIG. 5 is a fluorescent microscope photograph of the flow path 103 through which the sample solution flowed. At this stage, an alternating voltage was not applied.

[0048] The supply of the sample solution was continued, while an alternating voltage having a voltage of 14.14 volts pp and a frequency of 5MHz between the first pillar electrodes 301 and the second pillar electrodes 302 was applied through the first comb-shaped electrode 201 and the second comb-shaped electrode 202. FIG. 6 is a fluorescent microscope photograph of the flow path 103 through which the sample solution flowed while the alternating voltage was applied.

[0049] Finally, the application of the alternating voltage was stopped. The supply of the sample solution was continued. FIG. 7 is a fluorescent microscope photograph of the flow path 103 after five seconds of an end of the application of the alternating voltage.

[0050] As is clear from FIG. 5 - FIG. 7, even if the supply of the sample solution is continued, the particles are concentrated at and around the center point O during the application of the alternating voltage.

(Inventive example 2)



[0051] In the inventive example 2, an experiment similar to the inventive example 1 was conducted, except that the alternating voltage had a voltage of 7 volts pp and a frequency of 100 kilohertz. FIG. 8 - FIG. 10 are fluorescent microscope photographs which correspond to FIG. 5 - FIG. 7 respectively. As is clear from FIG. 8 - FIG. 10, also in the inventive example 2, even if the supply of the sample solution is continued, the particles are concentrated at and around the center point O during the application of the alternating voltage.

(Inventive example 3)



[0052] In the inventive example 3, an experiment similar to the inventive example 1 was conducted, except that the sample solution contained not fluorescence polystyrene particles but inactivated influenza virus particles and that the alternating voltage had a voltage of 10 volts pp and a frequency of 500 kilohertz. FIG. 11 is a fluorescent microscope photograph taken before an application of an alternating voltage. FIG. 12 is a fluorescent microscope photograph taken during the application of the alternating voltage. In both of FIG. 11 and FIG. 12, the supply of the sample solution was continued. As is clear from FIG. 11 and FIG. 12, also in the inventive example 3, even if the supply of the sample solution is continued, the influenza virus particles are concentrated at and around the center point O during the application of the alternating voltage.

[0053] The sample solution containing the inactivated influenza virus particles was prepared as below. The influenza virus was H1N1 type A/Hyogo/YS/2011 strain contained in allantoic fluid of a chicken egg cultured in Graduate School of Veterinary Medicine, Hokkaido University. The influenza virus was inactivated using β-propiolactone.

[0054] Then, the inactivated influenza virus was dyed as below. Fluorescent dye (from Biotium company, trade name: 30022 CellBriteOrange Cyvertexlasmic Membrane Dye 1 ml Dil cell labeling solution, 5 microliters) was diluted with 500 milliliters of saline. Soon after that, the saline containing the fluorescent dye is mixed with the aqueous solution of the inactivated influenza virus (500 milliliters). In this way, a mixture solution was obtained. The mixture solution was left at rest 37 degrees Celsius for 20 minutes. In this way, the inactivated influenza virus was dyed.

[0055] A mannitol aqueous solution (concentration: 280 mM, volume: 1 milliliter) was added to the aqueous solution of the inactivated influenza virus. The aqueous solution was filtered with a filter of 0.45 micrometers. In this way, impurities each having a diameter of not less than 0.45 micrometers were removed. Then, the aqueous solution was condensed with a centrifugal filter having filtration accuracy of the 100kDa molecular weight so as to have a volume of approximately 60 microliter. Finally, a mannitol aqueous solution (concentration: 280 mM, volume: 600 microliter) was added so that the aqueous solution had electrical conductivity of 0.78 mS/cm. In this way, the sample solution containing the inactivated influenza virus was prepared.

(Simulation example A1)



[0056] In the simulation example A1, the concentration of the particles was simulated under a condition shown in the following Table 2 using a simulator (available from COMSOL company, trade name: COMSOL Multiphysics).
[Table 2]
Number of Pillar Electrode Line 303 3
Number of First Pillar Electrodes 301 in each pillar electrode line 303 10
Number of Second Pillar Electrodes 302 in each pillar electrode line 303 10
L1 25.5 micrometers
L2 25.5 micrometers
P 2.8 micrometers
Q 31.1 micrometers
A1 29.7 micrometers
A2 1.4 micrometers
L3 28.3 micrometers
H 50 micrometers
W 280 micrometers
Top-view shape of First Pillar Electrode 301 Rugby ball
Top-view shape of Second Pillar Electrode 302 Rugby ball
Voltage 10 volts pp


[0057] FIG. 13 is a top view showing the simulation results in the simulation example A1. An electric field increases with an increase in the density of black dots. As is clear from FIG. 13, a region having a significantly high electric field is formed at and around the center point O.

(Simulation example A2)



[0058] In the simulation example A2, the simulation similar to the simulation example A1 was conducted, except that each of the first pillar electrodes 301 had a shape of a lozenge and a square in a top view. FIG. 14 is a top view showing the simulation results in the simulation example A2. As is clear from FIG. 14, a region having a significantly high electric field is formed at and around the center point O.

(Simulation example A3)



[0059] In the simulation example A3, the simulation similar to the simulation example A1 was conducted, except that each of the second pillar electrodes 302 had a shape of a lozenge and a square in a top view. FIG. 15 is a top view showing the simulation results in the simulation example A3. As is clear from FIG. 15, a region having a significantly high electric field is formed at and around the center point O.

[0060] As is clear from FIG. 13 - FIG. 15, the top-view shape of the first pillar electrodes 301 and the second pillar electrodes 302 is not limited, as far as the following four requirements (I) - (IV) are satisfied.
  1. (I) Each of the first pillar electrodes 301 includes the first vertex P1 and the second vertex P2.
  2. (II) Each of the second pillar electrodes 302 includes the first vertex Q1 and the second vertex Q2.
  3. (III) The line segment between the first vertex P1 and the second vertex P2 is parallel to the X-axis direction.
  4. (IV) The line segment between the first vertex Q1 and the second vertex Q2 is parallel to the Y-axis direction.

(Simulation example B1)



[0061] In simulation example B1, the dielectrophoresis force given to the particles was simulated under a condition shown in the following Table 3.
[Table 3]
Number of Pillar Electrode Lines 303 3
Number of First Pillar Electrodes 301 in each pillar electrode line 303 10
Number of Second Pillar Electrodes 302 in each pillar electrode line 303 10
L1 25.5 micrometers
L2 25.5 micrometers
P 2.8 micrometers
Q 31.1 micrometers
A1 29.7 micrometers
A2 1.4 micrometers
L3 28.3 micrometers, 5 micrometers, or 0 micrometers
H 50 micrometers
w 280 micrometers
Top-view shape of First Pillar Electrode 301 Rugby ball
Top-view shape of Second Pillar Electrode 302 Rugby ball
Particle size of the particle 100 nanometers
Voltage 10 volts pp


[0062]  In the simulation example B1, as shown in FIG.16, simulated was the dielectrophoresis force given to the particle located on the straight line LL which passes through the center point O and inclines at an angle of 45 degrees with regard to the line segment P. As shown in FIG. 16, when the particle is located in the +X-Y direction from the center point O, the distance between the particle and the center point O is defined as a negative value. On the other hand, when the particle is located in the -X+Y direction from the center point O, the distance between the particle and the center point O is defined as a positive value. Needless to say, the direction in which the sample solution flows through the flow path 103 is the +Y direction.

[0063] FIG. 17 is a graph showing the results of the simulation example B1. In the graph shown in FIG. 17, the horizontal axis represents the distance between the center point O and the particle located on the straight line LL. The vertical axis represents the dielectrophoresis force given to the particle. The positive value represents dielectrophoresis force as attractive force. The negative value represents dielectrophoresis force as repulsive force. In other words, the attractive force is given to the particle to draw the particle to the center point O in the upper region of the graph shown in FIG. 17. On the other hand, the repulsive force given to the particle to keep the particle away from the center point O in the lower region of the graph shown in FIG. 17.

[0064] As is clear from FIG. 17, if the value of L3 is not less than 5 micrometers, dielectrophoresis force is given to the particles as attractive force. Due to dielectrophoresis force as attractive force, the particles are captured only at and around the center point O against the stream of the fluid (namely, the sample solution) flowing through the flow path 103. In this way, the particles are concentrated only at and around the center point O.

[0065] On the other hand, if the value of L3 is 0 micrometers, dielectrophoresis force is given as repulsive force to the particles, while the particles approaches the center point O along the stream of the fluid (namely, the sample solution) flowing through the flow path 103. The dielectrophoresis force given as repulsive force accelerates the speed of the particles. For this reason, the particles fail to be captured and flow toward the lower course of the flow path 103. Therefore, the particles fail to be concentrated.

(Simulation example B2)



[0066] In the simulation example B2, a simulation similar to the simulation example B1 was carried out, except that the diameter of the particle was 30 nanometers. FIG. 18 is a graph showing the results of the simulation example B2.

[0067] As is clear from FIG. 18, if the value of L3 is not less than 5 micrometers, the dielectrophoresis force as the attractive force is given to the particles. Therefore, the particles are concentrated only at and around the center point O. On the other hand, if the value of L3 is 0 micrometers, the dielectrophoresis force is given as repulsive force to the particles, while the particles approaches the center point O along the stream of the fluid (namely, the sample solution) flowing through the flow path 103. Therefore, the particles fail to be concentrated.

(Simulation example B3)



[0068] In the simulation example B3, a simulation similar to the simulation example B1 was carried out, except that the alternating voltage had 5 volts pp. FIG. 19 is a graph showing the results of the simulation example B3.

[0069] As is clear from FIG. 19, if the value of L3 is not less than 5 micrometers, the dielectrophoresis force as the attractive force is given to the particles. Therefore, the particles are concentrated only at and around the center point O. On the other hand, if the value of L3 is 0 micrometers, the dielectrophoresis force is given as repulsive force to the particles, while the particles approaches the center point O along the stream of the fluid (namely, the sample solution) flowing through the flow path 103. Therefore, the particles fail to be concentrated.

(Simulation example B4)



[0070] In the simulation example B4, a simulation similar to the simulation example B1 was carried out, except that the alternating voltage had 20 volts pp. FIG. 20 is a graph showing the results of the simulation example B4.

[0071] As is clear from FIG. 20, if the value of L3 is not less than 5 micrometers, the dielectrophoresis force as the attractive force is given to the particles. Therefore, the particles are concentrated only at and around the center point O. On the other hand, if the value of L3 is 0 micrometers, the dielectrophoresis force is given as repulsive force to the particles, while the particles approaches the center point O along the stream of the fluid (namely, the sample solution) flowing through the flow path 103. Therefore, the particles fail to be concentrated.

(Simulation example B5)



[0072] In the simulation example B5, a simulation similar to the simulation example B1 was carried out, except that the value of A2 was 0.7 micrometers or 2.8 micrometers. FIG. 21 is a graph showing the results of the simulation example B5.

[0073] As is clear from FIG. 21, regardless of the value of A2, the dielectrophoresis force as the attractive force is given to the particles. Therefore, regardless of the value of A2, the particles are concentrated only at and around the center point O. The greater dielectrophoresis force as the attractive force is given to the particles with a decrease in the value of A2.

(Simulation example B6)



[0074] In the simulation example B6, a simulation similar to the simulation example B1 was carried out, except that the value of P was 0.7 micrometers or 2.8 micrometers. FIG. 22 is a graph showing the results of the simulation example B6.

[0075] As is clear from FIG. 22, regardless of the value of P, the dielectrophoresis force as the attractive force is given to the particles. Therefore, regardless of the value of P, the particles are concentrated only at and around the center point O. The greater dielectrophoresis force as the attractive force is given to the particles with a decrease in the value of P.

(Simulation example B7)



[0076] In the simulation example B7, a simulation similar to the simulation example B1 was carried out, except that the value of L1 was 25.5 micrometers or 39.6 micrometers. FIG. 23 is a graph showing the results of the simulation example B7.

[0077] As is clear from FIG. 23, regardless of the value of L1, the dielectrophoresis force as the attractive force is given to the particles. Therefore, regardless of the value of L1, the particles are concentrated only at and around the center point O.

INDUSTRIAL APPLICABILITY



[0078] The present invention can be used for a sensor for concentrating a virus having low concentration.

REFERENTIAL SIGNS LIST



[0079] 
101
Inlet
102
Outlet
103
Flow path
110
First substrate
105
Second substrate
201
First comb-shaped electrode
202
Second comb-shaped electrode
301
First pillar electrodes
301L Left-side first pillar electrode
301R Right-side first pillar electrode
302
Second pillar electrodes
302A Second pillar electrode A included in First pillar electrode line
302B Second pillar electrode B included in Second pillar electrode line
303a
First pillar electrode line
303b
Second pillar electrode line
303c
Third pillar electrode line
A1
Distance between Second vertex Q2 of Second pillar electrode A
A2
Distance between First vertex Q1 of Second pillar electrode B
H
Height of Flow path
L1
Length of First pillar electrode
L2
Length of Second pillar electrode
L3
Distance obtained by subtracting A2 from A1
LL
Line
O
Center line
P
Line segment
P1
First vertex of First pillar electrode
P2
Second vertex of First pillar electrode
Q
Line segment
Q1
First vertex of Second pillar electrode
Q2
Second vertex of Second pillar electrode
W
Width of Flow path
U
Region of Pillar electrode group



Claims

1. A concentration device suitable for dielectrophoresis, comprising:

a first substrate (110);

a second substrate (105) provided so as to face the first substrate (110);

a flow path (103) formed between the first substrate (110) and the second substrate (105);

a first pillar electrode line (303a) disposed in the flow path (103); and

a second pillar electrode line (303b) disposed in the flow path (103), wherein

the first pillar electrode line (303a) and the second pillar electrode line (303b) are parallel to an X-axis direction;

the first pillar electrode line (303a) and the second pillar electrode line (303b) include first pillar electrodes (301) and second pillar electrodes (302);

each of the first pillar electrodes (301) includes a first vertex P1 and a second vertex P2;

each of the second pillar electrodes (302) includes a first vertex Q1 and a second vertex Q2;

a line segment between the first vertex P1 and the second vertex P2 which are included in each of the first pillar electrodes 301 is parallel to the X-axis direction;

a line segment between the first vertex Q1 and the second vertex Q2 which are included in each of the second pillar electrodes 302 is parallel to a Y-axis direction;

the X-axis direction is perpendicular to the Y-axis direction in a top view;

a pillar electrode group is composed of a left-side first pillar electrode (301L) selected from the first pillar electrodes (301) included in the second pillar electrode line (303b);

a right-side first pillar electrode (301R) selected from the first pillar electrodes (301) included in the second pillar electrode line (303b);

a second pillar electrode (302A) selected from the second pillar electrodes (302) included in the first pillar electrode line (303a); and

a second pillar electrode (302B) selected from the second pillar electrodes (302) included in the second pillar electrode line (303b);

the left-side first pillar electrode (301L) and the right--side first pillar electrode (301R) are adjacent to each other in a top view in such a manner that the second vertex P2 of the left-side first pillar electrode (301L) and the first vertex P1 of the right-side first pillar electrode (301R) face each other;

a line which passes through the second vertex Q2 of the second pillar electrode (302A) and the first vertex Q1 of the second pillar electrode (302B) is parallel to the Y-axis direction; and

the following mathematical formula (I) is satisfied:

where

A1 represents a distance between the second vertex Q2 of the second pillar electrode (302A) and a center point O;

A2 represents a distance between the first vertex Q1 of the second pillar electrode (302B) and the center point O;

the center point O is an intersection point of a line segment P and a line segment Q,

the line segment Q is a line segment between the second vertex Q2 of the second pillar electrode (302A) and the first vertex Q1 of the second pillar electrode (302B); and

the line segment P is a line segment between the second vertex P2 of the left-side first pillar electrode (301L) and the first vertex P1 of the right-side first pillar electrode (301R).


 
2. A method for concentrating particles contained in a sample solution, the method comprising:

(a) preparing a concentration device according to Claim 1; and

(b) applying an alternating voltage between the first pillar electrodes (301) and the second pillar electrodes (302), while the sample solution is caused to flow in a direction from the first pillar electrode line (303a) to the second pillar electrode line (303b), to concentrate the particles only at and around the center point O.


 
3. The method according to Claim 2, wherein
each of the particles has a particle diameter of not more than 100 nanometers.
 
4. The method according to Claim 2, wherein
the alternating voltage has a voltage of not more than 20 volts pp.
 
5. The method according to Claim 2, wherein
the value of A2 is not more than 1.4 micrometers.
 
6. The method according to Claim 2, wherein
the line segment P has a length of not more than 2.8 micrometers.
 
7. The method according to Claim 2, wherein
the particles are influenza virus.
 
8. The method according to Claim 2, wherein
the particles are norovirus.
 


Ansprüche

1. Konzentrationsvorrichtung, die für eine Dielektrophorese geeignet ist, umfassend:

ein erstes Substrat (110),

ein zweites Substrat (105), das derart vorgesehen ist, dass es dem ersten Substrat (110) zugewandt ist,

einen Flusspfad (103), der zwischen dem ersten Substrat (110) und dem zweiten Substrat (105) ausgebildet ist,

eine erste Säulenelektrodenlinie (303a), die in dem Flusspfad (103) angeordnet ist, und

eine zweite Säulenelektrodenlinie (303b), die in dem Flusspfad (103) angeordnet ist,

wobei die erste Säulenelektrodenlinie (303a) und die zweite Säulenelektrodenlinie (303b) parallel zu einer X-Achsenrichtung sind,

wobei die erste Säulenelektrodenlinie (303a) und die zweite Säulenelektrodenlinie (303b) erste Säulenelektroden (301) und zweite Säulenelektroden (302) enthalten,

wobei jede der ersten Säulenelektroden (301) einen ersten Scheitel P1 und einen zweiten Scheitel P2 enthält,

wobei jede der zweiten Säulenelektroden (302) einen ersten Scheitel Q1 und einen zweiten Scheitel Q2 enthält,

wobei ein Liniensegment zwischen dem ersten Scheitel P1 und dem zweiten Scheitel P2 in jeder der ersten Säulenelektroden (301) parallel zu der X-Achsenrichtung ist,

wobei ein Liniensegment zwischen dem ersten Scheitel Q1 und dem zweiten Scheitel Q2 in jeder der zweiten Säulenelektroden (302) parallel zu einer Y-Achsenrichtung ist,

wobei die X-Achsenrichtung in einer Draufsicht senkrecht zu der Y-Achsenrichtung ist,

wobei eine Säulenelektrodengruppe besteht aus:

einer linken ersten Säulenelektrode (301L), die aus den ersten Säulenelektroden (301) in der zweiten Säulenelektrodenlinie (303b) ausgewählt ist,

einer rechten ersten Säulenelektrode (301R), die aus den ersten Säulenelektroden (301) in der zweiten Säulenelektrodenlinie (303b) ausgewählt ist

einer zweiten Säulenelektrode (302A), die aus den zweiten Säulenelektroden (302) in der ersten Säulenelektrodenlinie (303a) ausgewählt ist, und

einer zweiten Säulenelektrode (302B), die aus den zweiten Säulenelektroden (302) in der zweiten Säulenelektrodenlinie (303b) ausgewählt ist,

wobei die linke erste Säulenelektrode (301L) und die rechte erste Säulenelektrode (301R) in einer Draufsicht zueinander benachbart sind, sodass der zweite Scheitel P2 der linken ersten Säulenelektrode und der erste Scheitel P1 der rechten ersten Säulenelektrode (301R) einander zugewandt sind,

wobei eine Linie, die durch den zweiten Scheitel Q2 der zweiten Säulenelektrode (302A) und den ersten Scheitel Q1 der zweiten Säulenelektrode (302B) hindurchgeht, parallel zu der Y-Achsenrichtung ist, und

wobei die folgende mathematische Formel (I) erfüllt wird:

wobei

wobei A1 die Distanz zwischen dem zweiten Scheitel Q2 der zweiten Säulenelektrode (302A) und einem Mittenpunkt O wiedergibt,

wobei A2 die Distanz zwischen dem ersten Scheitel Q1 der zweiten Säulenelektrode (302B) und dem Mittenpunkt O wiedergibt,

wobei der Mittenpunkt O ein Kreuzungspunkt eines Liniensegments P und eines Liniensegments Q ist,

wobei das Liniensegment Q ein Liniensegment zwischen dem zweiten Scheitel Q2 der zweiten Säulenelektrode (302A) und dem ersten Scheitel Q1 der zweiten Säulenelektrode (302B) ist, und

das Liniensegment P ein Liniensegment zwischen dem zweiten Scheitel P2 der linken ersten Säulenelektrode (301L) und dem ersten Scheitel P1 der rechten ersten Säulenelektrode (301R) ist.


 
2. Verfahren zum Konzentrieren von Partikeln in einer Probenlösung, wobei das Verfahren umfasst:

(a) Vorbereiten einer Konzentrationsvorrichtung gemäß Anspruch 1, und

(b) Anlegen einer Wechselspannung zwischen den ersten Säulenelektroden (301) und den zweiten Säulenelektroden (302),

wobei veranlasst wird, dass die Probenlösung in einer Richtung von der ersten Säulenelektrodenlinie (303a) zu der zweiten Säulenelektrodenlinie (303b) fließt, um die Partikeln nur an und um den Mittenpunkt O herum zu konzentrieren.
 
3. Verfahren nach Anspruch 2, wobei jedes der Partikeln einen Partikeldurchmesser von nicht mehr als 100 Nanometer aufweist.
 
4. Verfahren nach Anspruch 2, wobei die Wechselspannung eine Spannung von nicht größer als 20 Volt pp ist.
 
5. Verfahren nach Anspruch 2, wobei der Wert von A2 nicht größer als 1,4 Mikrometer ist.
 
6. Verfahren nach Anspruch 2, wobei das Liniensegment P eine Länge von nicht größer als 2,8 Mikrometer aufweist.
 
7. Verfahren nach Anspruch 2, wobei die Partikeln Influenzaviren sind.
 
8. Verfahren nach Anspruch 2, wobei die Partikeln Noroviren sind.
 


Revendications

1. Dispositif de concentration convenant à une di-électrophorèse, comprenant :

un premier substrat (110),

un second substrat (105) disposé de sorte à faire face au premier substrat (110),

une voie d'écoulement (103) formée entre le premier substrat (110) et le second substrat (105),

une première ligne d'électrodes formant montant (303a) disposée dans la voie d'écoulement (103), et

une seconde ligne d'électrodes formant montant (303b) disposé dans la voie d'écoulement (103),

dans lequel

la première ligne d'électrodes formant montant (303a) et la seconde ligne d'électrodes formant montant (303b) sont parallèles à une direction d'axe X,

la première ligne d'électrodes formant montant (303a) et la seconde ligne d'électrodes formant montant (303b) incluent des premières électrodes formant montant (301) et des secondes électrodes formant montant (302),

chacune des premières électrodes formant montant (301) inclut un premier sommet P1 et un second sommet P2,

chacune des secondes électrodes formant montant (302) inclut un premier sommet Q1 et un second sommet Q2,

un segment de droite entre le premier sommet P1 et le second sommet P2 qui sont inclus dans chacune des premières électrodes formant montant (301) est parallèle à la direction d'axe X,

un segment de droite entre le premier sommet Q1 et le second sommet Q2 qui sont inclus dans chacune des secondes électrodes formant montant (302) est parallèle à une direction d'axe Y,

la direction d'axe X est perpendiculaire à la direction d'axe Y dans une vue de dessus,

un groupe d'électrodes formant montant est composé de :

une première électrode formant montant de côté gauche (301L) sélectionnée à partir des premières électrodes formant montant (301) incluses dans la seconde ligne d'électrodes formant montant (303b),

une première électrode formant montant de côté droit (301R) sélectionnée à partir des premières électrodes formant montant (301) incluses dans la seconde ligne d'électrodes formant montant (303b),

une seconde électrode formant montant (302A) sélectionnée à partir des secondes électrodes formant montant (302) incluses dans la première ligne d'électrodes formant montant (303a),

une seconde électrode formant montant (302B) sélectionnée à partir des secondes électrodes formant montant (302) incluses dans la seconde ligne d'électrodes formant montant (303b),

la première électrode formant montant de côté gauche (301L) et la première électrode formant montant deux côtés droit (301R) sont adjacentes l'une à l'autre dans une vue de dessus de manière à ce que le second sommet P2 de la première électrode formant montant de côté gauche (301L) et le premier sommet P1 de la première électrode formant montant de côté droit (301R) se fassent face,

une droite qui passe au travers du second sommet Q2 de la seconde électrode formant montant (302A) et au travers du premier sommet Q1 de la seconde électrode formant montant (302B) est parallèle à la direction d'axe Y, et

la formule mathématique (1) suivante est satisfaite :



A1 représente la distance entre le second sommet Q2 de la seconde électrode formant montant (302A) et un point central O,

A2 représente la distance entre le premier sommet Q1 de la seconde électrode formant montant (302B) et le point central O,

le point central O est le point d'intersection d'un segment de droite P et d'un segment de droite Q,

le segment de droite Q est un segment de droite entre le second sommet Q2 de la seconde électrode formant montant 302A et le premier sommet Q1 de la seconde électrode formant montant (302B), et

le segment de droite P est un segment de droite entre le second sommet P2 de la première électrode formant montant de côté gauche (301L) et le premier sommet P1 de la première électrode formant montant de côté droit (301R).


 
2. Procédé de concentration de particules contenues dans une solution échantillon, le procédé comprenant :

(a) la préparation d'un dispositif de concentration conforme à la revendication 1, et

(b) l'application d'une tension alternative entre les premières électrodes formant montant (301) et les secondes électrodes formant montant (302) alors que la solution échantillon est amenée à circuler dans une direction allant de la première ligne d'électrodes formant montant (303a) jusqu'à la seconde ligne d'électrodes formant montant (303b), afin de ne concentrer que les particules situées au niveau ou autour du point central O.


 
3. Procédé selon la revendication 2, dans lequel :
chacune des particules présentant un diamètre de particule non supérieur à 100 nanomètres.
 
4. Procédé selon la revendication 2, dans lequel :
la tension alternative présente une valeur non supérieure à 20 volts crête à crête.
 
5. Procédé selon la revendication 2, dans lequel :
la valeur de A2 n'est pas supérieure à 1,4 micromètres.
 
6. Procédé selon la revendication 2, dans lequel :
le segment de droite P présente une longueur non supérieure à 2,8 micromètres.
 
7. Procédé selon la revendication 2, dans lequel :
les particules sont le virus de la grippe.
 
8. Procédé selon la revendication 2, dans lequel :
les particules sont un norovirus.
 




Drawing

































































Cited references

REFERENCES CITED IN THE DESCRIPTION



This list of references cited by the applicant is for the reader's convenience only. It does not form part of the European patent document. Even though great care has been taken in compiling the references, errors or omissions cannot be excluded and the EPO disclaims all liability in this regard.

Patent documents cited in the description