(19) | |
| (11) | EP 3 543 335 A3 |
(12) | EUROPEAN PATENT APPLICATION |
(88) | Date of publication A3: | | 27.11.2019 Bulletin 2019/48 |
(43) | Date of publication A2: | | 25.09.2019 Bulletin 2019/39 |
(22) | Date of filing: 23.10.2015 |
| (51) | International Patent Classification (IPC): |
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(84) | Designated Contracting States: | | AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR |
(30) | Priority: | 03.11.2014 US 201462074494 P 24.08.2015 US 201562209010 P
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(62) | Application number of the earlier application in accordance with Art. 76 EPC: | | 15790411.1 / 3215614 |
(71) | Applicant: Merck Patent GmbH | | 64293 Darmstadt (DE) |
| (72) | Inventors: | | - ZILLMANN, Martin
Burlington, MA 01803 (US) - ORLANDO, Joe
Burlington, MA 01803 (US)
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(74) | Representative: J A Kemp LLP | | 14 South Square
Gray's Inn London WC1R 5JJ London WC1R 5JJ (GB) |
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(54) | SOLUBLE INTEIN FUSION PROTEINS AND METHODS FOR PURIFYING BIOMOLECULES |
(57) The present invention relates to fusion proteins comprising an N-intein polypeptide and an N-intein solubilization partner, and affinity chromatography matrices comprising such fusion proteins, as well as methods of using same.