(19)
(11)EP 3 543 335 A3

(12)EUROPEAN PATENT APPLICATION

(88)Date of publication A3:
27.11.2019 Bulletin 2019/48

(43)Date of publication A2:
25.09.2019 Bulletin 2019/39

(21)Application number: 19169588.1

(22)Date of filing:  23.10.2015
(51)Int. Cl.: 
C12N 9/50  (2006.01)
C07K 16/00  (2006.01)
C07K 14/00  (2006.01)
C12N 11/08  (2006.01)
C12N 9/00  (2006.01)
C07K 1/22  (2006.01)
C12P 21/00  (2006.01)
C12N 11/14  (2006.01)
(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30)Priority: 03.11.2014 US 201462074494 P
24.08.2015 US 201562209010 P

(62)Application number of the earlier application in accordance with Art. 76 EPC:
15790411.1 / 3215614

(71)Applicant: Merck Patent GmbH
64293 Darmstadt (DE)

(72)Inventors:
  • ZILLMANN, Martin
    Burlington, MA 01803 (US)
  • ORLANDO, Joe
    Burlington, MA 01803 (US)

(74)Representative: J A Kemp LLP 
14 South Square Gray's Inn
London WC1R 5JJ
London WC1R 5JJ (GB)

  


(54)SOLUBLE INTEIN FUSION PROTEINS AND METHODS FOR PURIFYING BIOMOLECULES


(57) The present invention relates to fusion proteins comprising an N-intein polypeptide and an N-intein solubilization partner, and affinity chromatography matrices comprising such fusion proteins, as well as methods of using same.