(19)
(11)EP 3 761 033 A3

(12)EUROPEAN PATENT APPLICATION

(88)Date of publication A3:
20.01.2021 Bulletin 2021/03

(43)Date of publication A2:
06.01.2021 Bulletin 2021/01

(21)Application number: 19217274.0

(22)Date of filing:  03.09.2014
(27)Previously filed application:
 03.09.2014 PCT/EP2014/068649
(51)Int. Cl.: 
G01N 33/68  (2006.01)
G16B 15/00  (2019.01)
(84)Designated Contracting States:
AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

(30)Priority: 06.09.2013 EP 13183389
12.09.2013 EP 13184174

(62)Application number of the earlier application in accordance with Art. 76 EPC:
14758893.3 / 3042205

(71)Applicant: F. Hoffmann-La Roche AG
4070 Basel (CH)

(72)Inventors:
  • KETTENBERGER, Hubert
    82377 Penzberg (DE)
  • KLOSTERMANN, Stefan
    82377 Penzberg (DE)
  • PAPADIMITRIOU, Apollon
    82377 Penzberg (DE)
  • LIPSMEIER, Florian
    82377 Penzberg (DE)
  • SYDOW-ANDERSEN, Jasmin
    82377 Penzberg (DE)

(74)Representative: Burger, Alexander et al
Roche Diagnostics GmbH Patentabteilung Nonnenwald 2
82377 Penzberg
82377 Penzberg (DE)

  


(54)METHOD FOR IMPROVING ANTIBODY STABILITY


(57) Herein is reported a method for selecting or deselecting an antibody comprising a) determining for each Asp and Asn residue in an antibody Fv region the conformational flexibility of the Ca-atom using a homology model ensemble, b) determining for each Asp and Asn residue in an antibody Fv region the size of the amino acid residue C-terminal to the Asp or Asn residue, and c) selecting an antibody in which the Cα-atom is conformationally inflexible and/or the Asp or Asn has a big C-terminal amino acid residue, or deselecting an antibody in which the Cα-atom has a moderate to high conformational flexibility and/or the Asp or Asn has a small C-terminal amino acid residue.