Description     Claims     Drawing  

Engineering yeast transcription machinery for improved ethanol tolerance and production   [0252] 
Application of bioluminescence to the study of circadian rhythms in cyanobacteria   [0252] 
Design of the linkers which effectively separate domains of a bifunctional fusion protein   [0252] 
Cyanobacterial two-component proteins: structure, diversity, distribution, and evolution   [0252] 
Ligation-independent cloning of PCR products (LI-PCR)   [0252] 
Minimal length requirement of the single-stranded tails for ligation-independent cloning (LIC) of PCR products   [0252] 
Evolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli   [0252] 
Direct photosynthetic recycling of carbon dioxide to isobutyraldehyde   [0252] 
Current topics in genome evolution: molecular mechanisms of new gene formation   [0252] 
Effect of butanol challenge and temperature on lipid composition and membrane fluidity of butanol tolerant Clostridium acetobutylicum   [0252] 
A phagemid vector using the E. coli phage shock promoter facilitates phage display of toxic proteins   [0252] 
The Escherichia coli cysG promoter belongs to the 'extended -10' class of bacterial promoters   [0252] 
Designer deletion strains derived from Saccharomyces cerevisiae S288C: a useful set of strains and plasmids for PCR-mediated gene disruption and other applications   [0252] 
Phage shock protein, a stress protein of Escherichia coli   [0252] 
Characterization and sequence of the Escherichia coli stress-induced psp operon   [0252] 
Expression of the psbDII gene in Synechococcus sp. strain PCC 7942 requires sequences downstream of the transcription start site   [0252] 
Light-regulated expression of the psbD gene family in Synechococcus sp. strain PCC 7942: evidence for the role of duplicated psbD genes in cyanobacteria   [0252] 
Multiple sequence alignment with the Clustal series of programs   [0252] 
Specialized techniques for site-directed mutagenesis in cyanobacteria   [0252] 
Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments   [0252] 
Construction and model-based analysis of a promoter library for E-coli: an indispensable tool for metabolic engineering   [0252] 
In vivo cloning by homologous recombination in yeast using a two-plasmid-based system   [0252] 
Ethanol synthesis by genetic engineering in cyanobacteria   [0252] 
Metabolic engineering of cyanobacteria for ethanol production   [0252] 
Tolerance and stress response to ethanol in the yeast Saccharomyces cerevisiae   [0252] 
Aquatic phototrophs: efficient alternatives to land-based crops for biofuels   [0252] 
Insertional inactivation of genes to isolate mutants of Synechococcus sp. strain PCC 7942: isolation of filamentous strains   [0252] 
Engineering cyanobacteria to generate high-value products   [0252] 
Rerouting carbon flux to enhance photosynthetic productivity   [0252] 
Engineering microbes for tolerance to next-generation biofuels   [0252] 
Engineering microbial biofuel tolerance and export using efflux pumps   [0252] 
Evolutionary mechanism as a template for protein engineering   [0252] 
An in vitro selection strategy for conferring protease resistance to ligand binding peptides   [0252] 
Genetic techniques appropriate for the biotechnological exploitation of cyanobacteria   [0252] 
Liquid gel amplification of complex plasmid libraries   [0252] 
Accurate variant detection across non-amplified and whole genome amplified DNA using targeted next generation sequencing   [0252] 
Gene transfer to cyanobacteria in the laboratory and in nature   [0252] 
The use of multiple displacement amplification to amplify complex DNA libraries   [0252] 
Vector systems for heterologous expression of proteins in Saccharomyces cerevisiae   [0252] 
Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria   [0252] 
Inducible expression of heterologous genes targeted to a chromosomal platform in the cyanobacterium Synechococcus sp. PCC 7942   [0252] 
Enzymatic assembly of DNA molecules up to several hundred kilobases   [0252] 
Chemical synthesis of the mouse mitochondrial genome   [0252] 
Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method   [0252] 
Yeast transformation by the LiAc/SS Carrier DNA/PEG method   [0252] 
High-efficiency yeast transformation using the LiAc/SS carrier DNA/PEG method   [0252] 
Why genes in pieces?   [0252] 
Genetic engineering of the cyanobacterial chromosome   [0252] 
Synthetic promoter libraries - tuning of gene expression   [0252] 
Rapid, reliable ligation-independent cloning of PCR products using modified plasmid vectors   [0252] 
Synthetic biology in cyanobacteria engineering and analyzing novel functions   [0252] 
Identification of gene targets eliciting improved alcohol tolerance in Saccharomyces cerevisiae through inverse metabolic engineering   [0252] 
Structural mutations in cancer: mechanistic and functional insights   [0252] 
In-fusion® cloning with vaccinia virus DNA polymerase   [0252] 
Expression of a gene cluster kaiABC as a circadian feedback process in cyanobacteria   [0252] 
Bio-based production of C2-C6 platform chemicals   [0252] 
Artificial promoters for metabolic optimization   [0252] 
The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters   [0252] 
Systematic engineering of microorganisms to improve alcohol tolerance   [0252] 
Identification, nucleotide sequence, and characterization of PspF, the transcriptional activator of the Escherichia coli stress-induced psp operon   [0252] 
Physiological tolerance and stoichiometric potential of cyanobacteria for hydrocarbon fuel production   [0252] 
Butanol tolerance in a selection of microorganisms   [0252] 
A versatile, efficient strategy for assembly of multi-fragment expression vectors in Saccharomyces cerevisiae using 60 bp synthetic recombination sequences   [0252] 
Circadian rhythms in prokaryotes: luciferase as a reporter of circadian gene expression in cyanobacteria   [0252] 
mRNA stability is regulated by a coding-region element and the unique 5' untranslated leader sequences of the three Synechococcus psbA transcripts   [0252] 
A period-extender gene, pex, that extends the period of the circadian clock in the cyanobacterium Synechococcus sp. strain PCC 7942   [0252] 
Metabolic engineering of cyanobacteria for 1-butanol production from carbon dioxide   [0252] 
ATP drives direct photosynthetic production of 1-butanol in cyanobacteria   [0252] 
Systems metabolic engineering of microorganisms for natural and non-natural chemicals   [0252] 
Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC   [0252] 
FastCloning: a highly simplified, purification-free, sequence- and ligation-independent PCR cloning method   [0252] 
SLIC: a method for sequence- and ligation-independent cloning   [0252] 
Activities of constitutive promoters in Escherichia coli   [0252] 
Engineering a platform for photosynthetic isoprene production in cyanobacteria, using Synechocystis as the model organism   [0252] 
How microbes tolerate ethanol and butanol   [0252] 
Fatty acid production in genetically modified cyanobacteria   [0252] 
Proteomic analysis reveals resistance mechanism against biofuel hexane in Synechocystis sp. PCC 6803   [0252] 
Enzymatic end-to end joining of DNA molecules   [0252] 
Plasmid construction by homologous recombination in yeast   [0252] 
Cyanobacterial biofuel production   [0252] 
Mechanisms of leukemogenesis by MLL fusion proteins   [0252] 
Chemicals from biobutanol: technologies and markets   [0252] 
The diversity of BCR-ABL fusion proteins and their relationship to leukemia phenotype   [0252] 
Constitutive versus thermoinducible expression of heterologous proteins in Escherichia coli based on strong PR,PL promoters from phage lambda   [0252] 
Fusion genes and rearranged genes as a linear function of chromosome aberrations in cancer.   [0252] 
The impact of translocations and gene fusions on cancer causation   [0252] 
The Escherichia coli phage-shock-protein (psp) operon   [0252] 
Biochemical properties of CikA, an unusual phytochrome-like histidine protein kinase that resets the circadian clock in Synechococcus elongatus PCC 7942   [0252] 
Targeted sequencing library preparation by genomic DNA circularization   [0252] 
A flexible approach for highly multiplexed candidate gene targeted resequencing   [0252] 
The Human OligoGenome Resource: a database of oligonucleotide capture probes for resequencing target regions across the human genome   [0252] 
Cyanobacterial conversion of carbon dioxide to 2,3-butanediol   [0252] 
Circular polymerase extension cloning of complex gene libraries and pathways   [0252] 
Circular polymerase extension cloning for high-throughput cloning of complex and combinatorial DNA libraries   [0252] 
Commonality but diversity in cancer gene fusions   [0252] 
General method for plasmid construction using homologous recombination   [0252] 
Linker-mediated recombinational subcloning of large DNA fragments using yeast   [0252] 
A new dawn for industrial photosynthesis   [0252] 
Engineered cyanobacteria: teaching an old bug new tricks   [0252] 
Molecular Cloning: A Laboratory Manual   [0252] 
The bcr-abl gene in chronic myelogenous leukaemia   [0252] 
DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways   [0252] 
Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector   [0252] 
Metabolic engineering of Saccharomyces cerevisiae for the production of n-butanol   [0252] 
Rapid evolution of a protein in vitro by DNA shuffling   [0252] 
DNA shuffling by random fragmentation and reassembly: in vitro recombination for molecular evolution   [0252] 
Construction and analysis of a recombinant cyanobacterium expressing a chromosomally inserted gene for an ethylene-forming enzyme at the psbAI locus   [0252] 
Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria   [0252] 
Microdroplet-based PCR enrichment for large-scale targeted sequencing   [0252] 
Quick and clean cloning: a ligation-independent cloning strategy for selective cloning of specific PCR products from nonspecific mixes   [0252] 
Quantitative proteomics reveals dynamic responses of Synechocystis sp. PCC 6803 to next-generation biofuel butanol   [0252] 
Overexpression of groESL in Clostridium acetobutylicum results in increased solvent production and tolerance, prolonged metabolism, and changes in the cell's transcriptional program   [0252] 
Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and chromosomal recombination   [0252] 
The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers   [0252] 
Transformation of cyanobacteria   [0252] 
Modular construction of plasmids through ligation-free assembly of vector components with oligonucleotide linkers   [0252] 
Enhancement of engineered trifunctional enzyme by optimizing linker peptides for degradation of agricultural by-products   [0252] 
Application of synthetic biology in cyanobacteria and algae   [0252] 
Single-step purification of shuttle vectors from yeast for high frequency back-transformation into E. coli   [0252] 
Stress-induced expression of the Escherichia coli phage shock protein operon is dependent on σ-54 and modulated by positive and negative feedback mechanisms   [0252] 
Analysis of the proteins and cis-acting elements regulating the stress-induced phage shock protein operon   [0252] 
Evolution of prokaryotic two-component systems: insights from comparative genomics   [0252] 
Reiterative Recombination for the in vivo assembly of libraries of multigene pathways   [0252] 
The bifunctional enzymes of antibiotic resistance   [0252] 
Expression of a cloned cyclopropane fatty acid synthase gene reduces solvent formation in Clostridium acetobutylicum ATCC 824   [0252] 
Designing and creating a modularized synthetic pathway in cyanobacterium Synechocystis enables production of acetone from carbon dioxide   [0252] 
Engineering cyanobacteria for fuels and chemicals production   [0252] 
Linker tailing: unphosphorylated linker oligonucleotides for joining DNA termini   [0252] 
In-fusion assembly: seamless engineering of multidomain fusion proteins, modular vectors, and mutations   [0252]