[0001] The present invention relates to novel cell lines and to ligands, namely human and/or
humanized monoclonal antibodies, as well as fragments such as Fab or single variable
domains and derivatives and combinations thereof, obtainable from the said cell line.
It also relates to pharmaceutical compositions comprising said ligands or antibody
fragments and to methods of preventing and treating haemostasis disorders, in particular
antithrombotic treatments in humans, by administration of the said ligands or antibody
fragments to patients in need thereof. It further relates to a polynucleotide encoding
for the antigen-binding Fab fragment of a monoclonal antibody derivable from the said
cell line.
BACKGROUND OF THE INVENTION
[0002] The coagulation of blood involves a cascading series of reactions leading to the
formation of fibrin. The coagulation cascade consists of two overlapping pathways
required for hemostasis. The intrinsic pathway comprises protein factors present in
circulating blood, while the extrinsic pathway requires tissue factor which is expressed
on the cell surface of a variety of tissues in response to vascular injury. Agents
interfering with the coagulation cascade, such as heparin and coumarin derivatives,
have well known therapeutic uses in the prophylaxis of venous thrombosis.
[0003] Aspirin also provides a protective effect against thrombosis. It induces a long-lasting
functional defect in platelets, detectable clinically as a prolongation of the bleeding
time, through inhibition of the cyclooxygenase activity of the human platelet enzyme
prostaglandin H-synthase (PGHS-1) with doses as low as 30 to 75 mg. Since gastrointestinal
side effects of aspirin appear to be dose-dependent, and for secondary prevention,
treatment with aspirin is recommended for an indefinite period, there are practical
reasons to choose the lowest effective dose. Further it has been speculated that a
low dose (30 mg daily) might be more antithrombotic but attempts to identify the optimal
dosage have yielded conflicting results. It has been claimed that the dose of aspirin
needed to suppress fully platelet aggregation may be higher in patients with cerebrovascular
disease than in healthy subjects and may vary from time to time in the same patient.
However, aspirin in any daily dose of 30 mg or higher reduces the risk of major vascular
events by 20 % at most, which leaves much room for improvement. Further, the inhibiting
role of aspirin may lead to prevention of thrombosis and to excess bleeding. The balance
between the two depends critically on the absolute thrombotic versus hemorrhage risk
of the patient.
[0004] In patients with acute myocardial infarction, reduction of infarct size, preservation
of ventricular function and reduction in mortality has been demonstrated with various
thrombolytic agents. However these agents still have significant shortcomings, including
the need for large therapeutic doses, limited fibrin specificity, and significant
associated bleeding tendency. Recombinant tissue plasminogen activator (t-PA) restores
complete patency in just over one half of patients, whereas streptokinase achieves
this goal in less than one third. Further, reocclusion after thrombolytic therapy
occurs in 5 to 10 % of cases during the hospital stay and in up to 30 % within the
first year according to Verheugt et al.,
J.
Am.
Coll.
Cardiol. (1996) 27:618-627. Numerous studies have examined the effects of adjunctive antithrombin
therapy for patients with acute myocardial infarction. For instance, U.S.Patent 5,589,173
discloses a method for dissolving and preventing reformation of an occluding thrombus
comprising administering a tissue factor protein antagonist, such as a monoclonal
or polyclonal antibody, in adjunction to a thrombolytic agent.
[0005] In arterial blood flow, the platelet adhesion is mainly supported by the platelet
receptor glycoprotein (GP) Ib which interacts with von Willebrand factor (vWF) at
the site of vessel wall injury. Blood platelets, through the processes of adhesion,
activation, shape change, release reaction and aggregation, form the first line of
defence when blood vessels are damaged. They form a hemostatic plug at the site of
injury to prevent excessive blood loss. Extensive platelet activation however may
overcome the normal thromboregulatory mechanisms that limit the size of the hemostatic
plug. Platelets then become major prothrombotic offenders predisposing to vaso-occlusive
disease.
[0006] The formation of a platelet plug during primary haemostasis and of an occluding thrombus
in arterial thrombosis involves common pathways. The first event is platelet adhesion
to subendothelial collagen, exposed upon vessel injury, which can be a ruptured atherosclerotic
plaque. Circulating vWF binds to the collagen and, under the influence of high shear
stress, undergoes a conformational change allowing it to bind to its receptor, GPIb/IX/V,
on the platelet membrane. This interaction is essential in order to produce a thrombus,
at least in smaller vessels or stenosed arteries where shear stress is high, and results
in slowing down the progress of the platelets across the damaged surface. Full immobilisation
of platelets occurs when collagen binds to its receptor GPIa/IIa (integrin α
2β
1). In addition, collagen activates platelets mainly by binding to GPVI, another collagen
receptor. When platelets are activated, GPIIb/IIIa (integrin α
IIBβ
3) undergoes a conformational change and acquires the ability to bind to fibrinogen
and vWF which crosslink adjacent platelets to finally form platelet aggregates.
[0007] Lately much effort has been directed to develop antibodies and peptides that can
block the binding of the adhesive proteins to GPIIb/IIIa and many of these are being
tested in clinical trials. One approach to blocking platelet aggregation involves
monoclonal antibodies specific for GPIIb/IIIa receptors. Specifically, a murine monoclonal
antibody named 7 E3 useful in the treatment of human thrombotic diseases is described
in EP-A-206,532 and U.S.Patent 5,387,413. However it is known in the art that murine
antibodies have characteristics which may severely limit their use in human therapy.
As foreign proteins, they may elicit an anti-immunoglobulin response termed human
anti-mouse antibody (HAMA) that reduces or destroys their therapeutic efficacy and/or
provokes allergic or hypersensitivity reactions in patients, as taught by Jaffers
et al.,
Transplantation (1986) 41:572. The need for readministration in therapies of thromboembolic disorders
increases the likelihood of such immune reactions. While the use of human monoclonal
antibodies would address this limitation, it has proven difficult to generate large
amounts of such antibodies by conventional hybridoma technology.
[0008] Recombinant technology has therefore been used to construct "humanized" antibodies
that maintain the high binding affinity of murine monoclonal antibodies but exhibit
reduced immunogenicity in humans. In particular, there have been suggested chimeric
antibodies in which the variable region (V) of a non-human antibody is combined with
the constant (C) region of a human antibody. As an example, the murine Fc fragment
was removed from 7E3 and replaced by the human constant immunoglobulin G region to
form a chimera known as c7E3 Fab or abciximab. Obtention of such chimeric immunoglobulins
is described in detail in U.S.Patent 5,770,198.
[0009] The potential for synergism between GPIIb/IIIa inhibition by monoclonal antibody
7E3 Fab and thrombolytic therapy was evaluated by Kleiman et al.,
J.
Am.
Coll.
Cardiol (1993) 22:381-389. Major bleeding was frequent in this study. Hence, the potential
for life-threatening bleeding is clearly a major concern with this combination of
powerful antithrombotic compounds.
[0010] The GPIb-vWF axis therefore presents an attractive alternative to GPIIb/IIIa-fibrinogen
as a target for platelet inhibition, since a suitable inhibitor might be expected
to down regulate other manifestations of platelet activity such as granule release,
thought to play a role in the development of arteriosclerosis. Activation of platelets
is accompanied by secretion of vasoactive substances (thromboxane A2, serotonin) as
well as growth factors such as PGDF. Therefore, early inhibition of platelet activation
and hence prevention of the secretion of their growth and migration factors, via a
GPIb blocker, would reduce the proliferation of smooth muscle cells and restenosis
after thrombolytic therapy. Despite these potential advantages, the development of
compounds that interfere with the vWF-GPIb axis has lagged behind. Only a few
in vivo studies described the effects of inhibition of platelet adhesion on thrombogenesis.
They include the use of anti-vWF monoclonal antibodies, GPIb binding snake venom proteins
like echicetin and crotalin, aurin tricarboxylic acid that binds to vWF and recombinant
vWF fragments like VCL, all of which inhibit vWF-GPIb interaction. All these molecules
were antithrombotic, particularly in studies where a thrombus was formed under high
shear conditions. A number of potent inhibitory anti-GPIb antibodies have been produced
and were extensively tested with respect to their
in vitro effect under both static (platelet agglutination, vWF-binding) and flow conditions.
J.L.Miller et al. In
Arterioscler.
Thromb. (1991) 11:1231-6 disclosed an
in vivo study on guinea pigs using F(ab')
2 fragments of PG1, a monoclonal anti-guinea pig GPIb antibody, which has shown to
effectively reduce thrombus formation on a laser-induced injury. Unfortunately, this
antibody does not cross react with human platelets. Part of this rather surprising
lack of
in vivo studies is due to the low cross reactivity of the anti-human GPIb monoclonal antibodies
with platelets from commonly used laboratory animals. This predisposes to the use
of non-human primates as experimental animals. However, even then attempts to perform
in vivo studies are hampered because injection of the anti-GPIb monoclonal antibodies, as
well as the snake venom protein echicetin that reacts with GPIb, invariably causes
severe thrombocytopenia.
[0011] One persistent concern with all available thrombolytic and anti-thrombotic agents,
including aspirin, is to induce a risk of overdose and therefore of excessive and
life-threatening bleeding. Therefore a first goal of the present invention is to provide
a thrombus formation protective means by providing a platelet adhesion inhibitor that
antagonizes human platelet glycoprotein Ib receptors without inducing a risk of bleeding.
A second goal of the present invention is to provide a thrombus formation protective
means by providing an inhibitor of platelet adhesion without incurring the risk of
thrombocytopenia.
SUMMARY OF THE INVENTION
[0012] The essence of this invention is that by using a ligand such as a monovalent Fab
fragment of a certain inhibitory human GPIb antibody, a marked prevention of platelet
dependent thrombus formation can be obtained without incurring thrombocytopenia.
[0013] The present invention therefore first includes a cell-line deposited with the Belgian
Coordinated Collections of Micro-organisms, under accession number LMBP 5108CB. Secondly
the present invention includes a ligand which binds to the human platelet glycoprotein
GPIb and prevents the binding of von Willebrand factor (vWF) to GPIb and which preferably
does not produce thrombocytopenia when administered to a primate at a dose of up to
640 µg/kg by bolus intravenous administration. In particular the present invention
includes a ligand derived from a monoclonal antibody such as 6B4 obtainable from the
said cell line. Thirdly the present invention relates to an antigen-binding Fab fragment,
or a homolog or derivative of such fragment, which may be obtained by proteolytic
digestion of the said monoclonal antibody by papain, using methods well known in the
art. Fourthly the present invention includes pharmaceutical compositions comprising
said ligands or fragments which are useful for preventing and treating haemostasis
disorders, in particular for anti-thrombotic treatments, in humans. Finally the present
invention includes polynucleotide sequences encoding for the above-mentioned monoclonal
antibodies or Fab fragments thereof. It will be appreciated that a multitude of nucleotide
sequences fall under the scope of the present invention as a result of the redundancy
in the genetic code. The present invention also includes nucleic acid molecules comprising
sequences which are complementary to the coding sequence of said polynucleotides and
the use of such molecules as DNA probes for detecting the said polynucleotides.
[0014] The present invention is first based on the observation of the antithrombotic effect
of human platelet glycoprotein GPIb blocking monoclonal antibody 6B4 Fab fragment
derived from the cell line LMBP 5108CB in a baboon model of arterial thrombosis. Baboons
were either pre-treated with said Fab fragment to study the effect on platelet deposition
on a thrombogenic device, or treated 6 minutes after placement of the thrombogenic
device in order to investigate the effect on inter-platelet cohesion. In this first
study, it was observed that blockade of GPIb had no effect on platelet deposition
onto a fresh thrombus, whereas pre-treatment effectively reduced thrombus formation.
[0015] Secondly, the present invention is based on
in vitro and
in vivo studies of the antithrombotic efficacy of the monoclonal antibody, 6B4 (IgG1), raised
against human platelet glycoprotein Ib.
In vitro, 6B4 potently inhibits the binding of vWF to human GPIb both under static and flow
conditions, as further illustrated by the following examples, and it also binds to
baboon platelets. When 6B4 was injected into baboons, both the intact monoclonal antibody
and its F(ab')
2 fragments caused immediate and severe thrombocytopenia, whereas Fab fragments of
6B4 did not. Further-more, Fab fragments studied in a baboon model of platelet-dependent
arterial thrombosis inhibited thrombosis when injected before a thrombus was generated
in baboons. When Fab-fragments were injected after a thrombus was allowed to form,
no inhibition of further thrombosis was observed.
BRIEF DESCRIPTION OF THE DRAWINGS
[0016]
Figure 1 shows the inhibiting effect of 6B4 Fab fragments on the ristocetin- and botrocetin-induced
binding of vWF to rGPIb.
Figure 2 shows the inhibiting effect of 6B4 Fab fragments on platelet adhesion to
collagen type I under flow.
Figure 3 shows binding curves of 6B4 and its fragments to baboon platelets in plasma.
Figure 4 shows the inhibitory effect of 6B4 and its fragments on ristocetin-induced
baboon platelet aggregation.
Figure 5 shows platelet adhesion and deposition onto three thrombogenic devices placed
in baboons either untreated (fig. 5A) or treated (fig. 5B) with 6B4 Fab fragments.
Figure 6 shows the influence of late treatment of baboons with 6B4 Fab fragments on
platelet deposition.
DEFINITIONS
[0017] The term "antibody" refers to intact molecules as well as fragments thereof, which
are capable of binding to the epitope determinant of the relevant factor or domain
of the factor.
[0018] "Humanized antibody" as used herein, refers to antibody molecules in which amino
acids have been replaced in the non-antigen binding regions in order to more closely
resemble a human antibody.
[0019] The term "homolog" as used herein with reference to ligands in accordance with the
present invention refers to a molecule which will compete with or inhibit binding
of one of the ligands in accordance with the present invention to the target site.
The binding should be specific, i.e. the binding of the alternative molecule should
be as specific to the site as the ligand in accordance with the present invention.
Where the ligands in accordance with the present invention include amino acid sequences,
homology may include having at least about 80%, more preferably about 90% and most
preferably about 95% amino acid sequence identity with the relevant ligand.
DETAILED DESCRIPTION OF THE INVENTION
[0020] The present invention will be described with reference to certain embodiments and
figures but the present invention is not limited thereto but only by the following
claims.
[0021] The present invention provides a cell-line deposited with the Belgian Coordinated
Collections of Micro-organisms, under accession number LMBP 5108CB. The present invention
further provides cell lines producing monoclonal antibodies having a reactivity, namely
a reactivity towards human GP Ib, substantially identical to that of monoclonal antibodies
obtainable or obtained from cell line LMBP 5108CB, as well as the human monoclonal
antibodies obtainable from the said further cell lines.
[0022] The present invention also provides ligands which are able to bind to the human platelet
glycoprotein GPIb and also preferably able to prevent the binding of von Willebrand
factor (vWF) to GPIb, in particular ligands derived from a monoclonal antibody (referred
to as 6B4) obtainable from said cell line LMBP 5108CB or from equivalent cell lines
such as above defined. More preferably, such a ligand should be able to recognize
an epitope located on human platelet glycoprotein GP Ib. For instance, the present
invention relates to ligands of the above-mentioned type, being derived from a monoclonal
antibody produced by on purpose immunization in animals. The present invention also
provides an antigen-binding Fab fragment, or a homolog or derivative of such fragment,
which may be obtained by proteolytic digestion of the said monoclonal antibody by
papain, using methods well known in the art. In order to reduce the immunogenicity
of the murine anti-GPIb monoclonal antibody 6B4, the present invention also includes
the construction of a chimeric antibody, preferentially as a single-chain variable
domain which combines the variable region of the mouse antibody with a human antibody
constant region - a so-called humanized monoclonal antibody. The monoclonal antibodies
produced in animals may be humanized, for instance by associating the binding complementarity
determining region ("CDR") from the non-human monoclonal antibody with human framework
regions - in particular the constant C region of human gene - such as disclosed by
Jones et al. in
Nature (1986) 321:522 or Riechmann in
Nature (1988) 332:323.
[0023] The present invention provides the use of a ligand or a humanized monoclonal antibody
or an antigen-binding Fab fragment such as specified hereinbefore as a medicament.
Although aspirin will continue to be widely used for patients with vascular disease,
however there are a number of situations in which increased thrombotic risk requires
the use of a more potent platelet inhibitor than aspirin. Conditions such as angioplasty,
coronary stenting and thrombolysis are likely to require more potent platelet inhibitors.
In these acute clinical situations, the fibrous cap over an atherosclerotic plaque
has been ruptured which produces deep arterial injury and exposes a much more thrombogenic
surface. Furthermore, high shear forces acting on platelets passing through severely
narrowed stenoses can also overcome the inhibitory effects of aspirin. Therefore a
GPIb antagonist according to the invention may be used for reducing the problems of
occlusion and restenosis in patients undergoing angioplasty or for the prevention
of reocclusion after succesful thrombolysis by tissue plasminogen activators, streptokinase
or the like. It is believed that platelet activation, as a result of the platelet
adhesion, is a key component in the failure of thrombolysis. Therefore a therapeutic
approach towards blocking the GPIb-vWF interaction, that results in a down-regulation
of platelet signalling, represents a new way of interfering in thrombus formation.
[0024] The present invention therefore further provides pharmaceutical compositions comprising
a ligand or a humanized monoclonal antibody or an antigen-binding Fab fragment such
as specified hereinbefore, in admixture with a pharmaceutically acceptable carrier.
More preferably the said pharmaceutical composition comprises a human or humanized
monoclonal antibody or an antigen-binding Fab fragment thereof obtainable from the
cell line LMBP 5108CB. which are useful for preventing and treating haemostasis disorders,
in particular for antithrombotic treatments, in humans.
[0025] The use of a GPIb blocker according to the present invention is believed to be more
efficient in acute situations and, in some cases, as an adjunctive therapy together
with other agents such as, among others, aspirin or heparin. The pharmaceutical composition
of the present invention may therefore further comprise, in view of the so-called
adjunctive therapy, a therapeutically effective amount of a thrombolytic agent. Such
thrombolytic agents, as well as their usual dosage depending on the class to which
they belong, are well known to those skilled in the art. Among numerous examples of
thrombolytic agents which may be included in the pharmaceutical compositions of the
invention, may be cited tissue plasminogen activators (t-Pa), streptokinase, reptilase,
TNK-t-Pa or staphylokinase. The pharmaceutical composition should comprise the additional
thrombolytic agent in a form which is suitable either for simultaneous use or for
sequential use. Sequential, as used herein, means that the ligand or humanized monoclonal
antibody or antigen-binding Fab fragment of the invention on the one hand and the
known thrombolytic agent are administered to the patient in alternance but not within
the same dosage unit.
[0026] Suitable pharmaceutical carriers for use in the pharmaceutical compositions of the
invention are described for instance in Remington's Pharmaceutical Sciences 16
th ed. (1980) and their formulation is well known to those skilled in the art. They
include any and all solvents, dispersion media, coatings, antibacterial and antifungal
agents (for example phenol, sorbic acid, chlorobutanol), isotonic agents (such as
sugars or sodium chloride) and the like. Additional ingredients may be included in
order to control the duration of action of the monoclonal antibody or Fab fragment
active ingredient in the composition. Control release compositions may thus be achieved
by selecting appropriate polymer earners such as for example polyesters, polyamino
acids, polyvinyl pyrrolidone, ethylene-vinyl acetate copolymers, methylcellulose,
carboxymethylcellulose, protamine sulfate and the like. The rate of drug release and
duration of action may also be controlled by incorporating the monoclonal antibody
active or Fab fragment ingredient into particles, e.g. microcapsules, of a polymeric
substance such as hydrogels, polylactic acid, hydroxymethylcellulose, polymethyl methacrylate
and the other above-described polymers. Such methods include colloid drug delivery
systems like liposomes, microspheres, microemulsions, nanoparticles, nanocapsules
and so on. Depending on the route of administration, the pharmaceutical composition
comprising the active ingredient may require protective coatings. The pharmaceutical
form suitable for injectionable use include sterile aqueous solutions or dispersions
and sterile powders for the extemporaneous preparation thereof. Typical carriers therefore
include biocompatible aqueous buffers, ethanol, glycerol, propylene glycol, polyethylene
glycol and mixtures thereof.
[0027] The pharmaceutical composition and medicament in accordance with the present invention
may be provided to a patient by means well known in the art. i.e. orally, intranasally,
subcutaneously, intramuscularly, intradermally, intravenously, intraarterially, parenterally
or by catheterization. For the reasons stated above, they will be especially useful
for the treatment and/or prevention of disorders of haemostasis and particularly for
antithrombotic treatment or prevention. Therefore the present invention further provides
a method of treatment and/or prevention of such disorders by administering to a patient
in need thereof a therapeutically effective amount of a ligand or a humanized monoclonal
antibody or an antigen-binding Fab fragment such as specified hereinbefore, optionally
together with (simultaneously or sequentially) a therapeutically effective amount
of a thrombolytic agent such as above described.
[0028] The present invention also provides a polynucleotide sequence encoding for the antigen-binding
Fab fragment, or homolog or derivative of the monoclonal antibody derived from cell
line LMBP 5108CB. The present invention also provides nucleic acid molecules comprising
a sequence which is complementary to the coding sequence of the said polynucleotide
and the use of such molecules as DNA probes for detecting the said polynucleotide.
[0029] The present invention is further described by the following examples which are provided
for illustration purposes only. Data were tested for statistically significant difference.
Data given in the text are mean ± SE. P-values < 0.05 are considered significantly
different.
Example 1- preparation and purification of intact monoclonal antibody 6B4. F(ab')2 and Fab fragments
[0030] 6B4 (subtype IgG1), is a murine monoclonal antibody raised against purified human
GPIb and obtainable from the cell line deposited with the Belgian Coordinated Collections
of Micro-organisms under accession number LMBP 5108CB When added at saturating concentrations,
monoclonal antibody 6B4 totally abolishes both ristocetin- and botrocetin-induced
human platelet aggregation as well as shear-induced platelet adhesion to human collagen
type I tested in a Sakariassen-type flow chamber at 2600s
-1.
[0031] Hybridoma cells producing the monoclonal antibody 6B4 were grown and subsequently
injected into pristane (i.e. 2,6,10,14-tetramethyldecanoic acid)-primed BaIb/c mice.
After 10 days ascites fluid was collected. The immunoglobulin (IgG) was extracted
from the ascites using protein-A-Sepharose CL-4B (available from Pharmacia, Roosendaal,
Netherlands).
[0032] In order to prepare F(ab')
2 fragments, the monoclonal antibody 6B4 was dialyzed overnight against a 0.1mol/l
citrate buffer (pH 3.5) The antibody (200 parts) was digested by incubation with pepsin
(1 part) available from Sigma (Saint-Louis, Missouri) for 1 hour at 37°C. Digestion
was stopped by adding 1 volume of a 1M Tris HCl buffer (pH 9) to 10 volumes of antibody.
[0033] Monovalent Fab fragments were prepared by papain digestion as follows: a 1 volume
of a 1M phosphate buffer (pH 7.3) was added to 10 volumes of the monoclonal antibody,
then 1 volume papain (Sigma) was added to 25 volumes of the phosphate buffer containing
monoclonal antibody, 10 mmol/l L-Cysteine HCl (Sigma) and 15 mmol/l ethylene diamine
tetra acetic acid (hereinafter referred to as EDTA). After incubation for 3 hours
at 37°C, digestion was stopped by adding a final concentration of 30 mmol/l freshly
prepared iodoacetamide solution (Sigma), keeping the mixture in the dark at room temperature
for 30 minutes.
[0034] Both F(ab')
2 and Fab fragments were further purified from contaminating intact IgG and Fc fragments
using protein-A-Sepharose. The purified fragments were finally dialyzed against phosphate-buffered
saline (hereinafter referred as PBS). Purity of the fragments was determined by sodium
dodecylsulphate polyacrylamide gel electrophoresis and the protein concentration was
measured using the bicinchonicic acid Protein Assay Reagent A (Pierce, Rockford, Illinois).
Example 2 - Method for determining deposition of platelets
[0035] Autologous blood platelets were labelled with
111In-tropolone and imaging and quantification of the deposition of
111In-platelets were done as described by Kotze et al ,
J.
Nucl.
Med. (1991) 32:62-66. Briefly, image acquisition of the grafts, including proximal and
distal silastic segments, was done with a Large Field of View scintillation camera
fitted with a high resolution collimator. The images were stored on and analysed with
a Medical Data Systems A
3 computer (Medtronic, Ann Arbor, MI) interfaced with the scintillation camera. Dynamic
image acquisition, 2 minute images (128x128 byte mode), was started simultaneously
with the start of blood flow through the devices. A two minute image (128x128 byte
mode) of a 3 ml autologous blood sample (collected in EDTA) was also acquired each
time that the grafts were imaged to determine circulating blood radioactivity (blood
standard). A region of interest of the graft segment was selected to determine the
deposited and circulating radioactivity in each of the dynamic images. Radioactivity
in a region of similar size of circulating radioactivity in the proximal segment of
the extension tubing was determined, and subtracted from the radioactivity in the
graft region to calculate deposited radioactivity. Platelet deposition was expressed
as the total number of platelets deposited. The method to calculate this is described
by Hanson et al,
Arteriosclerosis (1985) 5:595-603.
Example 3 - receptor binding measurements
[0036] 6B4, its F(ab')
2 or Fab fragments were labelled with Na-
125I (Amersham, Buckinghamshire, UK) using the Iodogen method as described by Fraker
et al.,
Biochem.
Biophys.
Res.
Comm. (1978) 80:849-857. Iodogen was purchased from Pierce (Rockford, IL). Platelet-rich
baboon plasma, adjusted with autologous plasma to a count of 100,000 platelets/µl,
was incubated with different concentrations of iodinated 6B4, F(ab')2 or Fab fragments
for 15 minutes at room temperature. The mixture was layered onto 20% sucrose buffer
(wt/vol) containing 0.1% (wt/vol) bovine serum albumin (BSA) and centrifuged for 4
min at 10,000g in Eppendorf tubes. The top fluid, including the plasma, was removed
and the pellets were counted in a gamma-counter. This study was performed in duplicate
on the platelet rich plasma of two baboons.
Example 4- In vitro and ex vivo platelet aggregation measurement
[0037] The aggregation of platelets in response to ristocetin (1.5 mg/ml final concentration;
abp, NY) was done on 10 ml blood collected in 1 ml of 3.2% trisodiumcitrate. Platelet
rich plasma was prepared by differential centrifugation as described by Van Wyk et
al,
Thromb.
Res. (1990) 57:601-9 and the platelet count adjusted to 200,000 platelets/µl with autologous
plasma. The aggregation response was measured in a Monitor IV Plus aggregometer (Helena
Laboratories, Beaumont, Texas) and recorded for 5 minutes. The percent aggregation
at 5 minutes was calculated as the difference in light transmission between platelet-rich
and platelet-poor plasma.
[0038] In
in vitro studies, the platelet rich plasma was preincubated for 5 minutes with serial dilutions
of intact IgG 6B4, F(ab')2 or Fab fragments before aggregation was initiated. Inhibition
of aggregation was calculated from the difference in the aggregation response of platelets
without and with antibody or fragments. In the
ex vivo determinations, inhibition was calculated from the difference in the aggregation
response of platelets before and after treatment of the baboons.
Example 5 - measurement of plasma concentrations of 6B4, F(ab')2 or Fab fragments and of bleeding time.
[0039] Plasma concentrations were measured using a sandwich enzyme-linked immunoassay (ELISA).
Briefly, microtiter plates were coated overnight at 4°C with 5 µg/ml polyclonal goat
anti-mouse IgG (Sigma). After blocking non-occupied binding sites with bovine serum
albumin, serial dilutions of baboon plasma were added to the wells and incubated for
two hours. Bound 6B4 (IgG, F(ab')
2 or Fab fragments) was detected by using goat anti-mouse IgG (Fab specific) conjugated
to peroxidase (Sigma). Standard curves were constructed by adding known amounts of
6B4 (IgG, F(ab')
2 or Fab fragments) to baboon plasma.
[0040] Bleeding time was determined using the Simplate® II device (Organon Teknika,Durham,
North Carolina) according to the instructions of the manufacturer, the volar surface
of the forearm of the baboons being shaved and a pressure cuff being applied and inflated
to 40 mm Hg.
Example 6 - In vitro effect of monoclonal antibody 6B4 and Fab fragments on binding of vWF to human GPIb
under static and flow conditions
[0041] Monoclonal antibody 6B4 binds to a (1-289) recombinant (r)GPIbα fragment expressed
by Chinese hamster ovary cells obtained from Meyer et al.
J.
Biol.
Chem. (1993) 268:20555-20562, indicating that its epitope is localized within the aminoterminal
region of GPIbα.
[0042] Monoclonal antibody 6B4 Fab fragments were further tested for inhibition of ristocetin-
and botrocetin-induced binding of vWF to the rGPIbα fragment using an ELISA set-up,
as described by Vanhoorelbeke et al.
Thromb.
Haemost. (2000):83:107-113. Microtiter plates were coated with 5 µg/ml monoclonal antibody
2D4 for 48 hours at 4°C. Monoclonal antibody 2D4, another anti-GPIb monoclonal antibody,
binds to the rGPIbα fragment but does not block vWF binding. Non-adsorbed sites were
blocked with 3% skimmed milk whereafter the plates were washed with tris buffered
saline (hereinafter referred as TBS) containing 0.1% Tween 20 (TBS-Tw). Purified rGPIbα
fragments were immobilised on monoclonal antibody 2D4 by incubating 2µg/ml rGPIbα
for 2 hours at 37 °C. After washing with TBS-Tw, increasing concentrations of 6B4
Fab fragments (diluted in TBS-Tw) were added, followed by 1.25 or 0.6 µg/ml purified
human vWF (available from the red Cross Belgium), respectively when ristocetin (300
µg/mL) or botrocetin (0.5 µg/mL) were used as modulators. Binding of vWF was determined
by incubating for 1 hour with HRP conjugated polyclonal anti-vWF antibody (Dako, Glostrup,
Denmark), diluted 1/3000 in TBS-Tw. The color reaction, stopped with 4 mol/l H
2SO
4 was generated with orthophenylenediamine (available from Sigma). The purification
of botrocetin from crude
Bothrops jararaca venom (available from Sigma) was performed according to Fujimura et al.
Biochemistry (1991) 30:1957-1964.
[0043] The effect of 6B4 Fab fragments on shear-induced platelet adhesion to collagen was
tested in a Sakariassen-type parallel-plate flow chamber at shear rates of 650, 1,300
and 2,600 sec
-1, according to Harsfalvi et al.
Blood (1995) 85:705-7011. Human collagen type I (Sigma) was dissolved in 50 mM acetic acid
(1 mg/ml), dialysed for 48 hours against PBS and subsequently sprayed onto plastic
Thermanox coverslips and stored at room temperature overnight before use. 12 ml of
blood, anticoagulated with LMW heparin (25 U/mL, Clexane, Rhône-Poulenc Rorer, France),
was preincubated with 6B4 Fab fragments at 37°C for 5 minutes and then used to perfuse
the collagen-coated coverslips. After 5 minutes of perfusion, the platelets were fixed
with methanol and the coverslips stained with May-Grünwald Giemsa. Platelet adhesion
(percent of total surface covered with platelets) was evaluated with a light microscope
connected to an image analyser. An average of 30 fields per coverslip were analysed.
Platelet adhesion was expressed as % maximal platelet adhesion obtained in the absence
of inhibitor.
[0044] Monoclonal antibody 6B4 Fab fragments block the ristocetin(1 mg/ml)-and botrocetin(0.5
µg/ml)-induced human platelet agglutination with an IC
50 of 1.2 ± 0.3 µg/ml (24 ± 6 nmol/l) and 2.0 ± 0.5 µg/mL (40 ± 10 nmol/L) respectively.
6B4 binds to an epitope localized on the aminoterminal part (His1-Val289) of GPIbα.
As shown in figure 1, the 6B4 Fab fragments dose-dependently inhibited both the ristocetin-
and botrocetin-induced binding of vWF to rGPIb, with an IC
50 of 1.8 µg/ml (36 nmol/l) and 2.5 µg/ml (50 nmol/l) respectively when the binding
was induced by ristocetin (300 µg/ml) or botrocetin (0.5 µg/ml).
[0045] As shown in figure 2, the 6B4 Fab fragments inhibited platelet adhesion to collagen
type I in a concentration-dependent manner at shear rates of 650, 1,300 and 2,600
sec
-1. A 50 % reduction of surface coverage was obtained at a concentration of 3.5 µg/mL
(70 nmol/l), 1.1 µg/mL (22 nmol/L) and 0.5 µg/mL (10 nmol/L) respectively for shear
rates of 650, 1,300 and 2,600 sec
-1.
Example 7 - in vivo studies in baboons : Dose response effect of 6B4 Fab-fragments on platelet adhesion
and deposition
[0046] Male baboons (
Papio ursinus) weighing between 10 and 15 kg and being disease-free for at least 6 weeks were used
according to procedures approved by the Ethics Committee for Animal Experimentation
of the University of the Orange Free State (South Africa) and the National Code for
Animal Use in Research, Education, Diagnosis and Testing of Drugs and Related Substances
(South Africa). The baboons supported permanent Teflon®-Silastic Arteriovenous (AV)
shunts implanted in the femoral vessels according to Hanson et al (cited
supra). Blood flow through the shunts varied between 100 and 120 ml/min, resulting in wall
shear rates between 800 and 1,000 sec
-1, which compares with the shear rates found in medium sized arteries. Handling of
the baboons was achieved through anaesthesia with about 10mg/kg ketamine hydrochloride
(Anaket-V, Centaur Laboratory, South Africa).
[0047] In order to test the effect of the monoclonal antibody on platelet count, 6B4 and
its F(ab')
2 and Fab fragments were administered to three different baboons. The injected dose
was calculated to attain a plasma concentration of 1xKD
50 i.e. the concentration needed to occupy 50% of the receptors as determined in
in vitro experiments.
[0048] Platelet-dependent arterial thrombus formation was induced by using bovine pericardium
(0.6 cm
2) fixed in buffered gluteraldehyde according to the method disclosed by Quintero et
al,
J.
Heart Valve Dis. (1998) 7:262-7. The pericardium was built into the wall of silicone rubber tubing
(3 mm inside diameter). The method of preparation of the thrombogenic device is described
by Kotze et al,
Thromb.
Haemost. (1993) 70:672-5, except that fixed bovine pericardium instead of Dacron® vascular
graft material was used. In each experiment, a thrombogenic device, prefilled with
saline to avoid a blood-air interface, was incorporated as an extension segment into
the permanent AV-shunt by means of Teflon® connectors as previously disclosed by Hanson
et al-(cited
supra).
[0049] In this first approach to determine the effect of 6B4 fragments on platelet adhesion,
seven baboons were used and thirteen perfusion experiments were performed. In the
first five experiments (3 baboons), a thrombogenic device was placed to determine
deposition of platelets according to the method of example 2. After 30 minutes, the
device was removed and blood flow through the permanent AV-shunt re-established. Fifteen
minutes after removal of the device, each baboon was treated with a bolus of 80 µg/kg
Fab fragments of 6B4 (in 2 ml saline) and again fifteen minutes later, a second thrombogenic
device was placed for 30 minutes to determine the effect of the Fab fragments on thrombogenesis.
The device was again removed and blood flow through the permanent shunt established.
This was followed by a second bolus injection of Fab fragments (80 µg/kg) to attain
a cumulative dose of 160 µg/kg. After fifteen minutes, a third thrombogenic device
was placed for 30 minutes and platelet deposition measured according to the method
of example 2. In four other experiments (2 baboons) the same study protocol was used
but two doses of 320µg/kg were administered.
[0050] In four other experiments (4 baboons), sham studies were performed by using the same
protocol of placement of thrombogenic devices, but the baboons were not treated with
Fab fragments.
[0051] Blood was collected at different time points (given in the figures) to determine
platelet count and haematocrit (EDTA), circulating and platelet associated radioactivity,
the
ex vivo aggregation of platelets in response to ristocetin (according to the method of example
4) and the plasma concentrations of Fab fragments (according to the method of example
5).
Example 8 - in vivo studies in baboons - Effect of anti-GPIb 6B4 fragments on interplatelet cohesion
[0052] In this second approach to determine the effect of 6B4 fragments on interplatelet
cohesion, six baboons were selected in a manner similar to that of example 7 and used
as follows. In all baboons, a thrombogenic device was placed for 24 minutes. In six
experiments (3 baboons), the baboons received a bolus injection of Fab fragments of
110 µg/kg. The fragments were injected six minutes after placement of the thrombogenic
device to allow enough platelets to be deposited to cover the collagen surface. In
the six other experiments, the other three baboons did not receive Fab fragments.
[0053] As in example 7, blood was collected at different time points (given in the figures)
to determine platelet count and haematocrit (EDTA), circulating and platelet associated
radioactivity, the
ex vivo aggregation of platelets in response to ristocetin (according to the method of example
4) and the plasma concentrations of Fab fragments (according to the method of example
5).
Experimental results
[0054] Figure 3 shows binding curves of anti-GPIb
125I-6B4 IgG (■), - F(ab')2 (●) and - Fab fragments (▲) to baboon platelets in plasma.
Binding of the antibody and its fragments to baboon platelets was dose-dependent and
saturable: half saturation (KD
50) was obtained with 4.7 nmol/l, 6.4 nmol/l and 49.2 nmol/l for the monoclonal antibody
6B4 IgG, its F(ab')
2 and Fab fragments respectively.
[0055] Figure 4 shows the inhibitory effect of anti-GPIb 6B4 IgG (■), - F(ab')2 (●) and
- Fab fragments (▲) on ristocetin-induced baboon platelet aggregation. When added
at saturating concentrations, ristocetin-induced aggregation was completely abolished:
IC
50-values were 4.5 nmol/l, 7.7 nmol/l and 40 nmol/l for the monoclonal antibody 6B4
IgG, its F(ab')
2 and Fab fragments respectively.
[0056] When considering the effect of injection of the monoclonal antibody 6B4, F(ab')
2 and Fab fragments on the peripheral platelet count in baboons, the dose of the 6B4
and its fragments used were calculated, for purposes of comparison to attain a plasma
concentration of 1xKD
50. In one baboon, 100 µg/kg of intact antibody caused a profound decrease in the blood
platelet count (< 30x10
9pl/l) within 10 minutes after injection. After 48 hours, the platelet count was still
below 100x10
9pl/l. When 6B4 F(ab')
2 fragments were injected into 2 baboons, the platelet count decreased rapidly to between
120 and 150x10
9pl/, i.e. by approximately 60%, and then reached pre-infusion values within 24 hours.
Finally when 80-320 µg/kg of the monovalent 6B4 Fab fragments was injected, the platelet
count (45 min after injection) decreased only by approximately 10-20% and by 26% when
640 µg/kg was injected as shown in table 1 hereinafter.
[0057] Figure 5 shows platelet deposition onto thrombogenic devices, containing bovine pericardium,
placed consecutively at times 0 (●), 60 (■) and 120 (▲) minutes for 30 minutes (top
shaded bars) for panel A and following injection of 0 (●), 80 (■) and 160 (▲), 320
(◆)and 640 (
)µg/kg 6B4 Fab fragments for panel B. In the sham studies (figure 5A), placement of
the previous graft had no significant effect on platelet deposition formed on subsequent
grafts. In the treatment studies (figure 5B), dosages of 80 µg/kg and 160 µg/kg significantly
inhibited platelet deposition in comparison to control, by approximately 43% and 53%
respectively. Doses of 320 µg/kg and 640 µg/kg significantly reduced platelet deposition
by 56% and 65% respectively.
[0058] Plasma levels of 6B4 Fab-fragments and inhibition of
ex vivo agglutination determined on samples obtained 45 minutes or 2 hours after administration
both changed dose- and time-dependently, as shown in table 1 hereinafter.
[0059] Bleeding times, determined in the treatment studies before and 45 minutes after injecting
80 to 320 µg/kg of 6B4 Fab fragments, were not significantly prolonged. Only a dose
of 640 µg/kg significantly prolonged the bleeding time which was still less than doubled.
[0060] Figure 6 shows the influence of late treatment of baboons with 6B4 Fab fragments
on platelet deposition, the thrombogenic device being placed at time 0 and platelet
deposition determined for 24 minutes (top shaded bar). After six minutes (arrow),
baboons were either untreated (■) or treated with a bolus of 110 (●) µg/kg 6B4 Fab
fragments. It is thus shown that 110 µg/kg 6B4 Fab fragment did not affect platelet
deposition when injected after a thrombus was allowed to form for an initial 6 minutes.
Interpretation of experimental results
[0061] The anti-GPIb monoclonal antibody 6B4, its F(ab')
2 and Fab fragments potently inhibited the binding of vWF to a recombinant GPIbα fragment
(His1-Val289) and dose-dependently inhibited vWF-dependent human platelet agglutination.
The intact monoclonal antibody and its fragments also dose-dependently inhibited human
platelet adhesion to type I collagen in a flow chamber at wall shear rates of 650,
1300 and 2600 sec
-1. This inhibition was shear-dependent, i.e. more pronounced at higher shear.
[0062] 6B4, its F(ab')
2 and Fab fragments also bind to and inhibit baboon platelets and inhibit baboon platelets
with much the same characteristics as human platelets. As a result baboons were used
for
in vivo and
ex vivo studies. An almost immediate, profound and irreversible thrombocytopenia developed
when the intact antibody was injected into a baboon, similar to what was observed
when other anti-GPIb monoclonal antibodies were injected into different experimental
animals. The F(ab')
2 fractions also caused immediate, but reversible thrombocytopenia, but to a lesser
extent than the intact antibody. The Fab fractions, on the other hand, had only a
moderate effect on the blood platelet count, which strongly suggests that the Fc portion
of the monoclonal antibody plays a part in the development of the irreversible thrombocytopenia.
[0063] The 6B4 Fab fractions were used to assess an anti-thrombotic effect in a baboon model
of arterial thrombosis. The gluteraldehyde fixed bovine pericardium was highly thrombogenic:
after 30 minutes of exposure to native flowing blood, approximately 3x10
9 platelets deposited on the area of 0.6 cm
2. In similar studies, only approximately 0.7x10
9 platelets accumulated on Dacron vascular graft material (0.9 cm
2) according to Kotzé et al.,
Thromb.
Haemost (1993) 70:672-675. It is therefore not surprising that a number of control thrombogenic
devices occluded before 30 minutes of exposure to flowing blood.
[0064] Treatment of baboons with 6B4 Fab fragments inhibited platelet deposition on the
thrombogenic devices by between 43 and 65%. The observed effect must be ascribed to
the monoclonal antibody, since sequential placement of thrombogenic devices in untreated
baboons caused no decreased deposition. No complete inhibition of platelet deposition
was observed, even at high doses.
1. Cell line deposited with the Belgian Coordinated Collections of Micro-organisms, under
accession number LMBP 5108CB.
2. A cell line producing monoclonal antibodies having a reactivity substantially identical
to that of the monoclonal antibodies obtained from the cell line of claim 1.
3. A ligand which binds to the human platelet glycoprotein GPIb and prevents the binding
of von Willebrand factor to said human GPIb.
4. A ligand according to claim 4, which does not produce thrombocytopenia when administered
to a primate at a dose of up to 640 µg/kg by bolus intravenous administration.
5. A ligand derived from a monoclonal antibody obtainable from the cell lines of claim
1 or claim 2.
6. A ligand according to claim 5, which binds to the human platelet glycoprotein GPIb.
7. A ligand according to claim 5 or claim 6, which prevents the binding of von Willebrand
factor to the human platelet glycoprotein GPIb.
8. A ligand according to any of claims 5 to 7, which does not produce thrombocytopenia
when
administered to a primate at a dose of up to 640 µg/kg by bolus intravenous administration.
9. A ligand according to any of claims 5 to 8, being a Fab fragment of the said monoclonal
antibody.
10. A ligand according to any of claims 5 to 9, being able to recognize an epitope located
on
human platelet glycoprotein GPIb.
11. A ligand according to any of claims 3 to 9 and being derived from a monoclonal antibody
produced by on purpose immunization in animals.
12. A humanized monoclonal antibody derivable from the monoclonal antibody of claim 11
or derivable from the cell lines of claims 1 or 2.
13. An antigen-binding Fab fragment or a homolog or derivative of a monoclonal antibody
according to claims 11 or 12 or derived from the cell lines of claims 1 or 2.
14. A pharmaceutical composition, comprising a ligand according to any of claims 3 to
11, a
humanized monoclonal antibody according to claim 12 or an antigen-binding Fab fragment
according to claim 13, in admixture with a pharmaceutically acceptable carrier.
15. A pharmaceutical composition according to claim 14, further comprising a thrombolytic
agent in a form either for simultaneous or sequential use.
16. Use of a ligand according to any of claims 3 to 11, a humanized monoclonal antibody
according to claim 12 or an antigen-binding Fab fragment according to claim 13 as
a medicament.
17. Use according to claim 16 in simultaneous or sequential association with at least
a thrombolytic agent.
18. Use according to claim 16 or claim 17 for the treatment and/or prevention of a disorder
of haemostasis.
19. Use according to any of claims 16 to 18, wherein the said medicament is for oral,
intranasal, subcutaneous, intramuscular, intradermal, intravenous, intraarterial or
parenteral administration or for catheterization.
20. A polynucleotide encoding for an antigen-binding Fab fragment according to claim 13.
21. A DNA probe for detecting the polynucleotide sequence of claim 20, comprising a nucleic
acid molecule having a sequence complementary to the coding sequence of said polynucleotide.