PROBES LABELLED WITH ENERGY TRANSFER COUPLED DYES

Description

INTRODUCTION

Technical Field

[0001] The field of this invention is fluorescent tags and their use.

Background

[0002] There is an increasing demand to be able to identify and quantify components of mixtures. The greater the complexity of the mixture, the greater the interest in being able to simultaneously detect a plurality of the components present. As illustrative of this situation is DNA sequencing, where it is desirable to efficiently excite from one to four fluorescently tagged components with a laser source at a single wavelength, while providing for fluorescent signal emission at a plurality of distinctive wavelengths. In this situation, the different labels should not adversely affect the electrophoretic mobility of the sequences to which they are attached.

[0003] Currently, there are four methods used for automated DNA sequencing: (1) the DNA fragments are labeled with one fluorophore and then the fragments run in adjacent sequencing lanes (Ansorge et al., Nucleic Acids Res. 15, 4593-4602 (1987); (2) the DNA fragments are labeled with four different fluorophores and all the fragments are electrophoretically separated and detected in a single lane (Smith et al., Nature 321, 674-679 (1986); (3) each of the dideoxynucleosides in the termination reaction is labeled with a different fluorophore and the four sets of fragments are run in the same lane (Prober et al., Science 238, 336-341 (1987); or (4) the sets of DNA fragments are labeled with two different fluorophores and the DNA sequences coded with the dye ratios (Huang et al., Anal. Chem. 64, 2149-2154, (1992).

[0004] All of these techniques have significant deficiencies. Method 1 has the potential problems of lane to lane variations in mobility, as well as a low throughput. Methods 2 and 3 require that the four dyes be well excited by one laser source and that they have distinctly different emission spectra. In practice, it is very difficult to find two or more dyes that can be efficiently excited with a single laser and that emit well separated fluorescent signals.

[0005] As one selects dyes with distinctive red-shifted emission spectra, their absorption spectra will also move to the red and all the dyes can no longer be efficiently excited by the same laser source. Also, as more different dyes are selected, it becomes more difficult to select all the dyes such that they cause the same mobility shift of the labelled molecules.

[0006] It is therefore of substantial interest that improved methods be provided which allow for multiplexing of samples, so that a plurality of components can be determined in the same system and in a single run. It is also desirable for each label to have a strong absorption at a common wavelength, to have a high quantum yield for fluorescence, to have a large Stokes' shift of the emission, that the various emissions be distinctive, and that the labels introduce the same mobility shift. It is difficult to accomplish these conflicting goals by simply labelling the molecules with a single dye.

[0007] US patent No.4996143 discloses polynucleotide probes containing donor and acceptor fluorophores for non-radiative energy transfer, such that neither the donor or acceptor moieties is attached at the 5' or 3' end units of the probe. The fluorophores are attached to the polynucleotide probes by linker arms. In one embodiment, the donor and acceptor fluorophores are attached to the probe which give them a relative separation of between two and seven intervening base units.

[0008] WO 93/09128 describes a polynucleotide having at least two (multiple) donor chromophores and a fluorescing acceptor fluorophore, positioned at a donor-acceptor transfer distance such that the multiple donors can collect excitation light and transfer it to the acceptor. In one example, efficient energy transfer is demonstrated in an oligonucleotide probe in which a terminal acceptor fluorescent group (Texas Red) is separated by one nucleotide unit from a donor group (fluorescein).

[0009] EP 439036 A describes an energy transfer system which includes the chromophore lumazin and a complex of ruthenium linked to a nucleotide chain.

[0010] Japanese Application No.H5-60698 discloses nucleic acid fragments, eg. primers, labelled with fluorescent bodies for use in the separation and detection of nucleic acids. In particular, single stranded DNA oligomers are labelled with two kinds of fluorescent bodies in an energy transfer relationship.

[0011] None of the above disclose combinations or families of fluorescent labels, comprising pairs of fluorophores and their use in separation systems involving a plurality of components.

SUMMARY OF THE INVENTION

[0012] The subject invention provides compositions and methods for analyzing a mixture using a plurality of fluorescent labels. To generate the labels, pairs or families of fluorophores are bound to a backbone, particularly a nucleic acid backbone, where one of the members of the families is excited at about the same wavelength. By exploiting the phenomenon of energy transfer, the other members of each of the families emit at detectably different wavelengths. The range of distances between donor and acceptor chromophores is chosen to ensure efficient energy transfer. Furthermore, labels used conjointly are selected to have approximately the same mobility in a separation system. This is achieved by changing the mobility of the labelled entity by varying the distance between the two or more members of the family of fluorophores and choosing labels with the same mobility. The subject invention finds particular application in sequencing, where the fluorophores may be attached to universal or other primers and different fluorophore combinations used for the different dideoxynucleosides. Kits of combinations of labels are also provided.

BRIEF DESCRIPTION OF THE DRAWINGS

[0013] 

Fig. 1 is a graph of the absorption and emission spectra of FAM-3-TAM in 1xTBE;

Fig. 2 is a CE electropherogram of FAM-3-TAM. The sample was analyzed by typical capillary electrophoresis DNA sequencing conditions with 488 nm excitation. The green trace is the fluorescence signal detected in the green channel (525 nm), and the red trace is the fluorescence signal detected in the red channel (590 nm). Both channels are detected simultaneously;

Fig. 3 is a graph of the absorption and emission spectra of FAM-4-ROX in 1xTBE;

Fig. 4 is a CE electropherogram of FAM-4-ROX. The sample was analyzed by typical capillary electrophoresis DNA sequencing conditions with 488 nm excitation. The green trace is the fluorescence signal detected in the green channel (525 nm), and the red trace is the fluorescence signal detected in the red channel (590 nm). Both channels are detected simultaneously;

Fig. 5 is a CE electropherogram of FAM-4-ROX and ROX primer. The two primers at the same concentration were mixed together in 80% formamide and injected into the capillary. The fluorescence signals were detected in the green and red channels simultaneously with 476 nm excitation;

Fig. 6 is a CE electropherogram of a FAM-3-ROX, FAM-4-ROX and FAM-10-ROX mixture, showing the dependence of the mobility on the distance between the donor and acceptor. The sample was analyzed by typical capillary electrophoresis DNA sequencing conditions with 488 nm excitation; and

Fig. 7 is a comparison of the mobility shift of different dye primers on M13 mp 18 A fragment DNA samples.

DESCRIPTION OF SPECIFIC EMBODIMENTS

[0014] Novel fluorescent labels, combinations of fluorescent labels, and their use in separation systems involving the separation of a plurality of components are provided.

[0015] Accordingly, the present invention provides a combination of fluorescent labels, each label comprising a donor-acceptor fluorescent pair wherein said donor and said acceptor are each covalently bonded to different atoms of a backbone chain of atoms with efficient energy transfer from said donor to said acceptor and wherein each of said labels is a single molecular species and wherein each of said labels in the combination absorbs at substantially the same wavelength and emits at a different wavelength.

[0016] Particularly, the fluorescent labels comprise pairs of fluorophores, which with one exception where the fluorophores are the same, involve different fluorophores having overlapping spectra, where the donor emission overlaps the acceptor absorption, so that there is energy transfer from the excited fluorophore to the other member of the pair. It is not essential that the excited fluorophore actually fluoresce, it being sufficient that the excited fluorophore be able to efficiently absorb the excitation energy and efficiently transfer it to the emitting fluorophore.

[0017] The donor fluorophores in the different families of fluoropbores may be the same or different, but will be able to be excited efficiently by a single light source of narrow bandwidth, particularly a laser source. The donor fluorophores will have significant absorption, usually at least about 10%, preferably at least about 20% of the absorption maxima within 20 nm of each other, usually within 10 nm, more usually within 5 nm, of each other. The emitting or accepting fluorophores will be selected to be able to receive the energy from donor fluorophores and emit light, which will be distinctive and detectably different. Therefore, one will be able to distinguish between the components of the mixture to which the different labels have been bound. Usually the labels will emit at emission maxima separated by at least 10 nm, preferably at least 15 nm, and more preferably at least 20 nm.

[0018] Usually the donor fluorophores will absorb in the range of about 350 - 800 nm, more usually in the range of about 350 - 600 nm or 500- 750 nm, while the acceptor fluorophores will emit light in the range of about 450 - 1000 nm, usually in the range of about 450 - 800 nm. As will be discussed subsequently, one may have more than a pair of absorbing molecules, so that one may have 3 or more molecules, where energy is transferred from one molecule to the next at higher wavelengths, to greatly increase the difference in wavelength between absorption and observed emission.

[0019] The two fluorophores will be joined by a backbone or chain, usually a polymeric chain, where the distance between the two fluorophores may be varied. The physics behind the design of the labels is that the transfer of the optical excitation from the donor to the acceptor depends on 1/R⁶, where R is the distance between the two fluorophores. Thus, the distance must be chosen to provide efficient energy transfer from the donor to the acceptor through the well-known Foerster mechanism. Thus, the distance between the two fluorophores as determined by the number of atoms in the chain separating the two fluorophores can be varied in accordance with the nature of the chain. Various chains or backbones may be employed, such as nucleic acids, both DNA and RNA, modified nucleic acids, e.g. where oxygens may be substituted by sulfur, carbon, or nitrogen, phosphates substituted by sulfate or carboxylate, etc., polypeptides, polysaccharides, various groups which may be added stepwise, such as di-functional groups, e.g. haloamines, or the like. The fluorophores may be substituted as appropriate by appropriate functionalization of the various building blocks, where the fluorophore may be present on the building block during the formation of the label, or may be added subsequently, as appropriate. Various conventional chemistries may be employed to ensure that the appropriate spacing between the two fluorophores is obtained.

[0020] The molecular weights of the labels (fluorophores plus the backbone to which they are linked) will generally be at least about 250 Dal and not more than about 5,000 Dal, usually not more than about 2,000 Dal. The molecular weight of the fluorophore will generally be in the range of about 250 to 1,000 Dal, where the molecular weights of the acceptor-donor pairs on different labels to be used together will usually not differ by more than about 20%. The fluorophores may be bound internal to the chain, at the termini, or one at one terminus and another at an internal site. The fluorophores may be selected so as to be from a similar chemical family, such as cyanine dyes, xanthenes or the like. Thus, one could have the donors from the same chemical family, each donor-acceptor pair from the same chemical family or each acceptor from the same family.

[0021] The subject labels find particular application in various separation techniques, such as electrophoresis, chromatography, or the like, where one wishes to have optimized spectroscopic properties, high sensitivity and comparable influence of the labels on the migratory aptitude of the components being analyzed. Of particular interest is electrophoresis, such as gel, capillary, etc. Among chromatographic techniques are HPLC, affinity chromatography, thin layer chromatography, paper chromatography, and the like.

[0022] It is found that the spacing between the two fluorophores will affect the mobility of the label. Therefore, one can use different dye pairs and by varying the distance between the different dye pairs, within a range which still permits good energy transfer, provide for substantially constant mobility for the labels. The mobility is not related to the specific spacing, so that one will empirically determine the effect of the spacing on the mobility of a particular label. However, because of the flexibility in the spacing of the fluorophores in the labels, by synthesizing a few different labels with different spacings and different dye pairs, one can now provide for a family of fluorescent labels, which share a common excitation, that have strong and distinctive emission and a substantially common mobility. Usually, the mobility will differ by not more than about 20% of each other, preferably not more than about 10% of each other, and more preferably within about 5% of each other, when used in a particular separation. The mobility may usually be determined by carrying out the separation of the labels by themselves or the labels bound to a common molecule which is relevant to the particular separation, e.g. a nucleic acid molecule of the appropriate size, where one is interested in sequencing.

[0023] A wide variety of fluorescent dyes may find application. These dyes will fall into various classes, where combinations of dyes may be used within the same class or between different classes. Included among the classes are dyes, such as the xanthene dyes, e.g. fluoresceins and rhodamines, coumarins, e.g. umbelliferone, benzimide dyes, e.g. Hoechst 33258, phenanthridine dyes, e.g. Texas Red, and ethidium dyes, acridine dyes, cyanine dyes, such as thiazole orange, thiazole blue, Cy 5, and Cyfr, carbazole dyes, phenoxazine dyes, porphyrin dyes, quinoline dyes, or the like. Thus, the dyes may absorb in the ultraviolet, visible or infra-red ranges. For the most part, the fluorescent molecules will have a molecular weight of less than about 2 kDal, generally less than about 1.5 kDal.

[0024] The energy donor should have strong molar absorbance coefficient at the desired excitation wavelength, desirably greater than about 10⁴, preferably greater than about 10⁵ cm^-1M^-1. The excitation maximum of the donor and the emission maximum of the acceptor (fluorescer) will be separated by at least 15 nm or greater. The spectral overlap integral between the emission spectrum of the donor chromophore and the absorption spectrum of the acceptor chromophore and the distance between the chromophores will be such that the efficiency of energy transfer from donor to acceptor will range from 20% to 100%.

[0025] Separation of the donor and acceptor based on number of atoms in the chain will vary depending on the nature of the backbone, whether rigid or flexible, involving ring structures or non-cyclic structures or the like. Generally the number of atoms in the chain (the atoms in the ring structures will be counted as the lowest number of atoms around one side of the ring for inclusion in the chain) will be below about 200, usually below about 150 atoms, preferably below about 100, where the nature of the backbone will influence the efficiency of energy transfer between donor and acceptor.

[0026] While for the most part, pairs of fluorophores will be used, there can be situations where up to four different, usually not more than three different, fluorophores bound to the same backbone may find use. By using more fluorophores, one may greatly extend the Stokes shift, so that one may excite in the visible wavelength range and emit in the infra-red wavelength range, usually below about 1000 nm, more usually below about 900 nm. Detecting light in the infra-red wavelength range has many advantages, since it will not be subject to interference from Raman and Rayleigh light resulting from the excitation light. In order to maintain the mobility constant, one may use the same number of fluorophores on the labels, having a multiplicity of the same fluorophore to match the number of fluorophores on labels having different fluorophores for the large Stokes shift.

[0027] The labels of the present invention may be used in applications which include detecting and distinguishing between various components in a mixture. The method comprises binding different labels to different components of interest of the multi-component mixture and detecting each of said labeled components by irradiating at the absorption wavelength of said donors and detecting the fluorescence of each of said labels.

[0028] The subject invention finds particular application with nucleic acid chains, where the nucleic acid chains find use as primers in sequencing, the polymerase chain reaction, particularly for sizing, or other system where primers are employed for nucleic acid extension and one wishes to distinguish between various components of the mixture as related to the particular labels. For example, in sequencing, universal primers may be employed, where a different pair of fluorophores are used for each of the different dideoxynucleosides used for the extension during sequencing.

[0029] A large number of nucleosides are available, which are functionalized, and may be used in the synthesis of polynucleotide. By synthesizing the subject nucleic acid labels, one can define the specific sites at which the fluorophores are present. Commercially available synthesizers may be employed in accordance with conventional ways, so that any sequence can be achieved, with the pair of fluorophores having the appropriate spacing. Where different primers have been used in PCR, each of the primers may be labeled in accordance with the subject invention, so that one can readily detect the presence of the target sequence complementary to each of the different primers. Other applications which may find use include identifying isozymes, using specific antibodies, identifying lectins using different polysaccharides, and the like.

[0030] The fluorescent labels of the invention may also be used in methods which include separating nucleic acid components in a multi-component mixture in which each of the different components of interest are labeled with different labels, wherein each label comprises a donor-acceptor fluorescent pair covalently bonded to different atoms of an oligonucleotide chain with efficient energy transfer from the donor to the acceptor; each of the labels is a single molecular species and each absorbs at substantially the same wavelength and emits at a different wavelength; and each of the different labels has substantially the same mobility in said separation as a result of varying the spacing of the donor-acceptor pair along said oligonucleotide chain. The method comprises binding different labels to different components of said multi-component mixture, separating said components into individual fractions, and detecting each of said labeled components by irradiating at the absorption wavelength of said donors and detecting the fluorescence of each of said labels.

[0031] As already indicated, the subject labels find particular use in sequencing. For example, universal primers may be prepared, where the primer may be any one of the universal primers, having been modified by bonding of the two fluorophores to the primer. Thus, various commercial primers arc available, such as primers from pUC/M13, λgt10, λgt11, and the like. See, Sambrook et all., Molecular Cloning: A Laboratory Manual, 2nd ed., CSHL, 1989, Section 13. DNA sequences are cloned in an appropriate vector having a primer sequence joined to the sequence to be sequenced. Different 2', 3' ddNTPs are employed, so that termination occurs at different sites, depending upon the particular ddNTP which is present in the chain extension. By employing the subject primers, each ddNTP will be associated with a particular label. After extension with the Klenow fragment, the resulting fragments may then be separated in a single lane by electrophoresis or in a single capillary by electrophoresis, where one can detect the terminating nucleotide by virtue of the fluorescence of the label.

[0032] One may also use the subject labels with immune complexes, where the ligands or receptors, e.g. antibodies, may be labeled to detect the different complexes or members of the complexes. Where the ligands may have the same migratory aptitude in the method separation, to determine the presence of one or more of such ligands, the different antibodies could be labeled with the different labels fluorescing at different wavelengths, so as to be detectable, even where there is overlap of the compositions in the separation.

[0033] Kits are provided having combinations of labels, usually at least 2. Each of the labels will have the acceptor-donor pair, usually with comparable backbones, where the labels will be separated along the backbone to give comparable mobility in the separation method to be used. Each of the labels in a group to be used together will absorb at about the same wavelength and emit at different wavelengths. Each of the labels in the group will have about the same effect on mobility in the separation method, as a result of the variation in placement of the different fluorophores along the backbone.

[0034] The kits will generally have up to about 6, usually about up to about 4 different labels which are matching, but may have 2 or more sets of matching labels, having 2-6 different labels.

[0035] Of particular interest are labels comprising a nucleic acid backbone, where the labels will generally have at least about 10 nucleotides and not more than about 50 nucleotides, usually not more than about 30 nucleotides. The labels may be present on the nucleotides which hybridize to the complementary sequence or may be separated from those nucleotides. The fluorophores will usually be joined to the nucleotide by a convenient linking arm of from about 2 to 20, usually 4 to 16 atoms in the chain. The chain may have a plurality of functionalities, particularly non-oxo-carbonyl, more particularly ester and amide, amino, oxy, and the like. The chain may be aliphatic, alicyclic, aromatic, heterocyclic, or combinations thereof, usually comprising carbon, nitrogen, oxygen, sulfur, or the like in the chain.

[0036] The entire nucleic acid sequence may be complementary to the 5' primer sequence or may be complementary only to the 3' portion of the sequence. Usually, there will be at least about 4 nucleotides, more usually at least about 5 nucleotides which are complementary to the sequence to be copied. The primers are combined with the sequence to be copied in the appropriate plasmid having the primer sequence at the 3' end of the strand to be copied and dNTPs added with a small amount of the appropriate ddntp. After extension, the DNA may be isolated and transferred to a gel or capillary for separation.

[0037] The kits which are employed will have at least two of the subject labels, which will be matched by having substantially the same absorption for the donor molecule, distinct emission spectra and substantially the same mobility. Generally for single stranded nucleic acids, the separation will be from about 1-15, more usually 1-12, preferably about 2-10 nucleosides between fluorophores.

[0038] The following examples are offered by way of illustration and not by way of limitation.

Experimental

Design and Synthesis of Energy Transfer Fluorescent Dye Tagged Oligonucleotide Labels for Genetic Analysis.

[0039] Deoxyoligonucleotides (12-base long) with the sequence 5'-GTTTTCCCAGTC-3', selected from the M13 universal primer, were synthesized with donor-acceptor fluorophore pairs separated by different distances. Specifically, the 12-mer contains a modified base introduced by the use of 5'dimethoxytrityl-5-[N-(trifluoroacetylaminohexy)-3-acrylimido]-2'-deoxyUridine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite (Amino-Modifier C6 dT) (Structure 1), which has a primary amine linker arm at the C-5 position.

The donor dye was attached to the 5' side of the oligomer, and the acceptor dye was attached to the primary amine group on the modified T. The distances between the donor and acceptor were changed by varying the position of the modified T on the oligomer. The primers are denoted as D-N-A, where D is the donor, A is the acceptor and N is the number of bases between D and A. In all the primers prepared, D is Applied Biosystems Inc. ("ABI") dye FAM, a fluorescein derivative, A is ABI dyes TAM or ROX which are both rhodamine derivatives. As a representative example, the structure of FAM-3-TAM is shown below (Structure 2).

[0040] The advantages of the energy transfer approach described here are (1) that a large Stokes shift and much stronger fluorescence signals can be generated when exciting at 488 nm and (2) that the mobility of the primers can be tuned by varying the distances between the donor and acceptor to achieve the same mobility. The visible spectrum of FAM-3-TAM has both the absorption of FAM (495 nm) and TAM (560 nm); however with excitation at 488 nm nearly all of the emission comes out from T with a maximum at 579 nm (Fig. 1). This demonstrates efficient fluorescence energy transfer from FAM to TAM. This can also be seen by running the primer down a capillary electrophoresis (CE) column and detecting in red and green channels. With a FAM- and TAM-labeled primer, nearly all the emission is seen in the red channel (590 nm) (Fig. 2), indicating that the energy from donor FAM was transferred almost completely to the acceptor TAM, producing a Stokes shift of 91 nm. The observation of a single peak indicates the primer is pure. The same outcome is seen for FAM-4-ROX, which gives even a larger Stokes shift of 114 nm (Figs. 3 and 4). Enhancement of the fluorescence signals of the energy transfer primers compared to single dye labeled primer is seen, where an ABI ROX primer at the same concentration as that of FAM-4-ROX (measured by UV) was injected in the same capillary. The resulting fluorescence signal of FAM-4-ROX is seen to be more than ten times higher than that of the ROX primer (Fig. 5).

[0041] For the successful application of donor-acceptor fluorophore labeled primers to DNA sequencing, it is essential that the primers produce the same mobility shifts of the DNA fragments and display distinct fluorescence signals. It was found that the mobility of the primers depends on the distance between the donor and acceptor (Fig. 6). FAM-4-ROX, FAM-3-ROX and FAM-10-ROX were separated on a capillary and detected in red and green channels. For FAM-10-ROX the increased distance between the dyes reduces the amount of energy transfer, resulting in almost equal signals in the two channels. As the separation distance is reduced, the amount of energy transfer increases as evidenced by the reduced relative green signal. FAM-3-ROX and FAM-4-ROX both exhibit excellent energy transfer, but their mobilities are distinctly different, which offers the potential of tuning the mobility shift by varying the distance. To get an exact match of the mobility of two primers that have distinctly different emission spectra, FAM-3-FAM, FAM-4-FAM and FAM-10-FAM were also prepared. Among a library of primers prepared (FAM-N-FAM, FAM-N-TAM, FAM-N-ROX), it was found that sequencing fragments terminating in A, generated with FAM-10-FAM and FAM-3-ROX using Sequenase 2, have very similar mobility shifts (Fig. 7), demonstrating the potential for DNA sequence analysis. The emission of FAM-10-FAM and FAM-3-ROX are at 525 nm and 605 nm respectively. The water Raman signals are trivial at these two wavelengths. Thus, the signal to noise ratio is increased dramatically.

I. Preparation of 12-mer Oligonucleotides Containing a Modified T and a FAM Label at the 5' Position.

[0042] The following three primers were prepared on an ABI Model 394 DNA synthesizer in a 0.2 µmol scale:





The modified base T* containing an amino linker arm was introduced to the defined position by using Amino-Modifier C6 dT phosphoramidite (Glen Research) and FAM was introduced by using 6-FAM amidite (ABI) in the last step of the synthesis. After the base sequences were completed, the oligonucleotides were cleaved from the solid support (CPG) with 1 ml concentrated NH₄OH. The amino protecting groups on the bases (A, G, C and T*) were removed by heating the NH₄OH solution for 4 hours at 55°C. Capillary electrophoresis analysis indicated that the oligomers were ∼80% pure, and they were used directly in the next dye-coupling step.

II. Attachment of the Second Fluorescent Dye to the Amino Linker Arm of the Oligomers 1, 2 and 3.

[0043] As a representative example, the reaction scheme to couple the second dye (TAM) to the oligomer 1 is shown below:

The FAM-labeled oligonucleotides (1, 2 and 3) in 40 µL 0.5 M Na₂CO₃/NaHCO₃ buffer were incubated overnight at room temperature with approximately 150 fold excess of either TAM-NHS ester, ROX-NHS ester or FAM-NHS ester in 12 µL DMSO. Unreacted dye was removed by size exclusion chromatography on a Sephadex G-25 column. The two dye labeled oligonucleotides were then purified by 6 M urea-TBE, 20% acrylamide gel electrophoresis (40 cm x 0.8 cm). The pure primers were recovered from the gel and desalted with Oligonucleotide Purification Cartridge. The purity of the primers was shown to be >99% by capillary gel electrophoresis.

III. Preparation of DNA Sequencing Fragments with FAM-3-ROX and FAM-10-FAM.

[0044] M13mp18 DNA sequencing fragments terminated in A were produced using Sequenase 2.0 (USB). Two annealing solutions were prepared in 600 µL vials: (1) 10 µL of reaction buffer, 40 µL of M13mp18 single-stranded DNA, and 6 µL of FAM-3-ROX; (2) 6 µL of reaction buffer, 20 µL of M13mp18 single-stranded DNA and 3 µL FAM-10-FAM. Each vial was heated to 65°C for 5min and then allowed to cool to room temperature for 30 min, and the placed on ice for 20 min to ensure that the shorter primers had completely hybridized to the template. 3 µL DTT , 20 µL of ddA termination mixture and 12 µL diluted Sequenase 2.0 were added to each vial on ice. The reaction mixtures were incubated initially at 20°C for 20 min and then at 37°C for another 20 min. Reactions were stopped by adding 10 µL 50 mM EDTA, 40 µL 4 M NH₄OH, and 300 µL 95% EtOH. The solutions were mixed well and then placed on ice for 20 min. The fragments were desalted twice with 75% cold EtOH, dried under vacuum and dissolved in 4 µL of 95% (v/v) formamide and 50 nM EDTA. The sample was heated for 3 min to denature the DNA and then placed on ice until sample injection on the capillary electrophoresis instrument. Electrokinetic injection was performed at 10 kV for 30 s.

[0045] It is evident from the above results, that one can tune related compositions, e.g. polynucleotides functionalized with 2 fluorophores to provide for different emission wavelengths and high emission quantum yields, while having substantially the same excitation-light absorbance and mobility. In this way, mixtures of compositions may be independently analysed, where the different compositions may be differently labelled with labels having differing fluorescence emission bands. Furthermore, the compositions can be readily prepared, can be used in a wide variety of contexts, and have good stability and enhanced fluorescent properties.

Claims

1. A combination of fluorescent labels, each label comprising a donor-acceptor fluorescent pair wherein said donor and said acceptor are each covalently bonded to different atoms of a backbone chain of atoms with efficient energy transfer from said donor to said acceptor and wherein each of said labels is a single molecular species and wherein each of said labels in the combination absorbs at substantially the same wavelength and emits at a different wavelength.

2. A combination according to claim 1 wherein said donor fluorophores are the same.

3. A combination according to claim 1 or 2 wherein each of said donor fluorophores absorbs light in the wavelength range of 350-800 nm and each of said acceptors emits light in the wavelength range of 450-1000 nm.

4. A combination according to claims 1 to 3 wherein the molecular weight of said labels (fluorophores plus the backbone chain of atoms to which they are linked) is not more than 5000 Daltons.

5. A combination according to claims 1 to 4 wherein said donor - acceptor pairs are xanthene dyes.

6. A combination according to claim 5 wherein said xanthene dyes comprise fluorescein derivatives and rhodamine derivatives.

7. A combination according to claims 1 to 6 wherein said backbone chain of atoms is a polymeric backbone.

8. A combination according to claim 7 wherein said polymeric backbone is an oligonucleotide.

9. A combination according to claims 1-8 wherein one or more of said fluorescent labels further comprises a third fluorophore such that energy is transferred from one molecule to the next at higher wavelength and excitation of the first fluorophore produces fluorescence from the third fluorophore.

10. A method of identification and detection of components in a multi-component mixture employing fluorescent labels to detect at least two components of interest, wherein:

i) each of said labels comprises a donor-acceptor fluorescent pair covalently bonded to different atoms of a backbone chain of atoms with efficient energy transfer from said donor to said acceptor; and

ii) each of the labels is a single molecular species and each absorbs at substantially the same wavelength and emits at a different wavelength;

said method comprising:

binding different labels to different components of interest of said multi-component mixture and detecting each of said labeled components by irradiating at the absorption wavelength of said donors and detecting the fluorescence of each of said labels.

11. A method according to claim 10 wherein said donor fluorophores are the same.

12. A method according to claim 10 wherein each of said donors absorbs light in the wavelength range of 350-800 nm and each of said acceptors emits light in the wavelength range of 450-1000 nm.

13. A method according to claim 12 wherein said donor-acceptor pair are xanthene dyes.

14. A method according to claim 13, wherein said xanthene dyes comprise fluorescein derivatives and rhodamine derivatives.

15. A method of separating components in a multi-component mixture wherein each of the components of interest are labeled with different labels, wherein said labels are characterized in that:

i) each label comprises a donor-acceptor fluorescent pair covalently bonded to different atoms of an oligonucleotide chain with efficient energy transfer from said donor to said acceptor;

ii) each of the labels is a single molecular species and each absorbs at substantially the same wavelength and emits at a different wavelength; and

iii) each of the different labels has substantially the same mobility in said separation as a result of varying the spacing of said donor-acceptor pair along said oligonucleotide chain;

said method comprising:

binding different labels to different components of said multi-component mixture; separating said components into individual fractions; and detecting each of said labeled components by irradiating at the absorption wavelength of said donors and detecting the fluorescence of each of said labels.

16. A method according to claim 15 wherein said separation is by electrophoresis.

17. A method according to claim 15 wherein said donor fluorophores are the same.

18. A method according to claim 15 wherein said donor absorbs light in the wavelength range of 350-800 nm and said acceptor emits light in the wavelength range of 450-1000 nm.

19. A method according to claim 15 wherein said donor-acceptor pair are xanthene dyes.

20. A method according to claim 19 wherein said xanthene dyes comprise fluorescein derivatives and rhodamine derivatives.

21. A method for sequencing a nucleic acid which employs primers for copying a single stranded nucleic acid and dideoxynucleotides for terminating the chain at a particular nucleotide resulting from said copying, said method comprising:

i) cloning a nucleic acid fragment to be sequenced into a vector comprising a primer binding sequence 5' to said fragment complementary to a primer;

ii) copying said fragment with a DNA polymerase in the presence of said primer, dNTPs and each of a plurality of dideoxynucleotides in separate reaction vessels, to generate single stranded DNA sequencing fragments;

iii) separating the resulting mixture of single stranded DNA sequencing fragments and determining the sequence by means of the bands present on the gel;

said DNA sequencing fragments comprising primers which have a donor-acceptor fluorescent pair covalently bonded to different atoms of a backbone chain of atoms with efficient energy transfer from said donor to said acceptor wherein each of said primers is a single molecular species, and each absorbs at substantially the same wavelength and emits at a different wavelength and wherein each of the primers has substantially the same mobility in said separation, resulting from varying the spacing and fluorophores of said donor-acceptor pair along said nucleic acid chain.

22. A method according to claim 21 wherein one of the members of said donor-acceptor fluorescent pair is bonded to the 5' terminus of said primer.

23. A method according to claim 21 wherein there are four primers having different donor-acceptor pairs.

24. A method according to claim 21 wherein said donor-acceptor fluorescent pair is separated by not more than 30 nucleotides.

25. A method according to claim 21 wherein at least two donor-acceptor fluorescent pairs are xanthene dyes.

26. A method according to claim 25 wherein said xanthene dyes comprise fluorescein derivatives and rhodamine derivatives.

27. A kit for use in any one of the methods according to claims 10-26 comprising a combination of different fluorescent labels according to any one of claims 1-9.

Ansprüche

1. Kombination aus fluoreszierenden Markern, wobei jeder Marker ein Donor-Akzeptor-Fluoreszenzpaar umfaßt, wobei der Donor und der Akzeptor jeweils kovalent an verschiedene Atome einer Grundgerüstkette von Atomen mit effizienter Energieübertragung von dem Donor auf den Akzeptor gebunden sind, und wobei jeder der Marker eine einzelne Molekülspezies ist, und wobei jeder der Marker in der Kombination bei im wesentlichen der gleichen Wellenlänge absorbiert und bei einer verschiedenen Wellenlänge emittiert.

2. Kombination nach Anspruch 1, wobei die Donor-Fluorophore die gleichen sind.

3. Kombination nach Anspruch 1 oder 2, wobei jedes der Donor-Fluorophore Licht im Wellenlängenbereich von 350-800 nm absorbiert, und jeder der Akzeptoren Licht im Wellenlängenbereich von 450-1000 nm emittiert.

4. Kombination nach den Ansprüchen 1 bis 3, wobei das Molekulargewicht der Marker (Fluorophore plus die Grundgerüstkette von Atomen, an welche sie gebunden sind) nicht mehr als 5000 Daltons beträgt.

5. Kombination nach den Ansprüchen 1 bis 4, wobei die Donor-Akzeptor-Paare Xanthen-Farbstoffe sind.

6. Kombination nach Anspruch 5, wobei die Xanthen-Farbstoffe Fluoresceinderivate und Rhodaminderivate umfassen.

7. Kombination nach den Ansprüchen 1 bis 6, wobei die Grundgerüstkette von Atomen ein polymeres Grundgerüst ist.

8. Kombination nach Anspruch 7, wobei das polymere Grundgerüst ein Oligonukleotid ist.

9. Kombination nach den Ansprüchen 1-8, wobei einer oder mehrere der fluoreszierenden Marker weiterhin ein drittes Fluorophor umfaßt, so dass Energie von einem Molekül zu dem nächsten bei höherer Wellenlänge übertragen wird und die Anregung des ersten Fluorophors Fluoreszenz von dem dritten Fluorophor produziert.

10. Verfahren zum Identifizieren und Detektieren von Komponenten in einer Mehrkomponentenmischung unter Verwendung von fluoreszierenden Markern, um mindestens zwei Komponenten von Interesse zu detektieren, wobei:

i) jeder der Marker ein Donor-Akzeptor-Fluoreszenzpaar umfaßt, das kovalent an verschiedene Atome einer Grundgerüstkette von Atomen mit effizienter Energieübertragung von dem Donor auf den Akzeptor gebunden ist; und

ii) jeder der Marker eine einzelne Molekülspezies ist und jeder bei im wesentlichen der gleichen Wellenlänge absorbiert und bei einer verschiedenen Wellenlänge emittiert;

   wobei das Verfahren umfaßt:

Binden verschiedener Marker an verschiedene Komponenten von Interesse der Mehrkomponentenmischung und Detektieren jeder der markierten Komponenten durch Bestrahlen bei der Absorptionswellenlänge des Donors und Detektieren der Fluoreszenz jedes der Marker.

11. Verfahren nach Anspruch 10, wobei die Donor-Fluorophore die gleichen sind.

12. Verfahren nach Anspruch 10, wobei jeder der Donoren Licht im Wellenlängenbereich von 350-800 nm absorbiert und jeder der Akzeptoren Licht im Wellenlängenbereich von 450-1000 nm emittiert.

13. Verfahren nach Anspruch 12, wobei das Donor-Akzeptor-Paar Xanthen-Farbstoffe sind.

14. Verfahren nach Anspruch 13, wobei die Xanthen-Farbstoffe Fluoresceinderivate und Rhodaminderivate umfassen.

15. Verfahren zum Trennen von Komponenten in einer Mehrkomponentenmischung, wobei jede der Komponenten von Interesse mit verschiedenen Markern markiert wird, wobei die Marker dadurch gekennzeichnet sind, dass

i) jeder Marker ein Donor-Akzeptor-Fluoreszenzpaar umfaßt, das kovalent an verschiedene Atome einer Oligonukleotidkette mit effizienter Energieübertragung von dem Donor auf den Akzeptor gebunden ist:

ii) jeder der Marker eine einzelne Molekülspezies ist und jeder bei im wesentlichen der gleichen Wellenlänge absorbiert und bei einer verschiedenen Wellenlänge emittiert; und

iii) jeder der verschiedenen Marker im wesentlichen die gleiche Mobilität bei der Trennung besitzt, als ein Ergebnis der Variation des räumlichen Abstandes des Donor-Akzeptor-Paars entlang der Oligonukleotidkette;

   wobei das Verfahren umfaßt:

Binden verschiedener Marker an verschiedene Komponenten der Mehrkomponentenmischung; Trennen der Komponenten in einzelne Fraktionen; und Detektieren jeder der markierten Komponenten durch Bestrahlen bei der Absorptionswellenlänge der Donoren und Detektieren der Fluoreszenz jedes der Marker.

16. Verfahren nach Anspruch 15, wobei die Trennung durch Elektrophorese erfolgt.

17. Verfahren nach Anspruch 15, wobei die Donor-Fluorophore die gleichen sind.

18. Verfahren nach Anspruch 15, wobei der Donor Licht im Wellenlängenbereich von 350-800 nm absorbiert und der Akzeptor Licht im Wellenlängenbereich von 450-1000 nm emittiert.

19. Verfahren nach Anspruch 15, wobei das Donor-Akzeptor-Paar Xanthen-Farbstoffe sind.

20. Verfahren nach Anspruch 19, wobei die Xanthen-Farbstoffe Fluoresceinderivate und Rhodaminderivate umfassen.

21. Verfahren zum Sequenzieren einer Nukleinsäure, bei dem Primer zum Kopieren einer einsträngigen Nukleinsäure und Didesoxynukleotide für die Termination der Kette an einem bestimmten Nukleotid, das aus dem Kopieren resultiert, eingesetzt werden, wobei das Verfahren umfasst:

i) Klonen eines zu sequenzierenden Nukleinsäurefragments in einen Vektor, umfassend eine Primer-Bindesequenz 5' zu dem Fragment, welches zu einem Primer komplementär ist;

ii) Kopieren des Fragments mit einer DNA-Polyermerase in Gegenwart des Primers, dNTPs und jeweils einer Vielzahl von Didesoxynukleotiden in separaten Reaktionsbehältern, um einsträngige DNA-Sequenzierungsfragmente zu erzeugen;

iii) Trennen der resultierenden Mischung aus einsträngigen DNA-Sequenzierungsfragmenten und Bestimmen der Sequenz mittels der auf dem Gel vorhandenen Banden;

   wobei die DNA-Sequenzierungsfragmente Primer umfassen, welche ein Donor-Akzeptor-Fluoreszenzpaar kovalent an verschiedene Atome einer Grundgerüstkette von Atomen mit effizienter Engergieübertragung von dem Donor auf den Akzeptor gebunden haben, wobei jeder der Primer eine einzelne Molekülspezies ist, und jeder bei im wesentlichen der gleichen Wellenlänge absorbiert und bei einer verschiedenen Wellenlänge emittiert, und wobei jeder der Primer im wesentlichen die gleiche Mobilität bei der Trennung besitzt, resultierend aus der Variation des räumlichen Abstandes und der Fluorophore des Donor-Akzeptor-Paars entlang der Nukleinsäurekette.

22. Verfahren nach Anspruch 21, wobei eines der Mitglieder des Donor-Akzeptor-Fluoreszenzpaares an das 5'-Ende des Primers gebunden ist.

23. Verfahren nach Anspruch 21, wobei vier Primer mit verschiedenen Donor-Akzeptor-Paaren vorhanden sind.

24. Verfahren nach Anspruch 21, wobei das Donor-Akzeptor-Fluorescenzpaar durch nicht mehr als 30 Nukleotide getrennt ist.

25. Verfahren nach Anspruch 21, wobei mindestens zwei Donor-Akzeptor-Fluoreszenzpaare Xanthen-Farbstoffe sind.

26. Verfahren nach Anspruch 25, wobei die Xanthen-Farbstoffe Fluoreszeinderivate und Rhodaminderivate umfassen.

27. Kit zur Verwendung in irgendeinem der Verfahren nach den Ansprüchen 10-26, umfassend eine Kombination aus verschiedenen fluoreszierenden Markern gemäß mindestens einem der Ansprüche 1-9.

Revendications

1. Combinaison de marqueurs fluorescents, chaque marqueur comprenant une paire fluorescente donneur-accepteur, dans laquelle ledit donneur et ledit accepteur sont chacun liés par covalence à des atomes différents d'une chaîne d'atomes formant un squelette avec un transfert d'énergie efficace dudit donneur audit accepteur, et dans laquelle chacun desdits marqueurs est une espèce moléculaire unique et dans laquelle chacun desdits marqueurs dans la combinaison absorbe à sensiblement la même longueur d'onde et émet à une longueur d'onde différente.

2. Combinaison selon la revendication 1, dans laquelle lesdits fluorophores donneurs sont les mêmes.

3. Combinaison selon la revendication 1 ou 2, dans laquelle chacun desdits fluorophores donneurs absorbe la lumière dans l'intervalle de longueurs d'onde de 350-800 nm et chacun desdits accepteurs émet de la lumière dans l'intervalle de longueurs d'onde de 450-1000 nm.

4. Combinaison selon les revendications 1 à 3, dans laquelle la masse moléculaire desdits marqueurs (fluorophores plus la chaîne d'atomes formant un squelette à laquelle ils sont liés) n'est pas supérieure à 5000 daltons.

5. Combinaison selon les revendications 1 à 4, dans laquelle lesdites paires donneur-accepteur sont des colorants de type xanthène.

6. Combinaison selon la revendication 5, dans laquelle lesdits colorants de type xanthène comprennent des dérivés de fluorescéine et des dérivés de rhodamine.

7. Combinaison selon les revendications 1 à 6, dans laquelle ladite chaîne d'atomes formant un squelette est un squelette polymérique.

8. Combinaison selon la revendication 7, dans laquelle ledit squelette polymérique est un oligonucléotide.

9. Combinaison selon les revendications 1-8, dans laquelle un ou plusieurs desdits marqueurs fluorescents comprend en outre un troisième fluorophore tel que l'énergie soit transférée d'une molécule à la suivante à une longueur d'onde plus élevée et que l'excitation du premier fluorophore produise une fluorescence par le troisième fluorophore.

10. Méthode d'identification et de détection de composants dans un mélange multi-composants employant des marqueurs fluorescents pour détecter au moins deux composants d'intérêt, dans laquelle :

i) chacun desdits marqueurs comprend une paire fluorescente donneur-accepteur liée par covalence à des atomes différents d'une chaîne d'atomes formant un squelette avec un transfert d'énergie efficace dudit donneur audit accepteur ; et

ii) chacun desdits marqueurs est une espèce moléculaire unique et chacun absorbe à sensiblement la même longueur d'onde et émet à une longueur d'onde différente ;

ladite méthode comprenant le fait :

de lier différents marqueurs à différents composants d'intérêt dudit mélange multi-composants et de détecter chacun desdits composants marqués en irradiant à la longueur d'onde d'absorption desdits donneurs et en détectant la fluorescence de chacun desdits marqueurs.

11. Méthode selon la revendication 10, dans laquelle lesdits fluorophores donneurs sont les mêmes.

12. Méthode selon la revendication 10, dans laquelle chacun desdits donneurs absorbe la lumière dans l'intervalle de longueurs d'onde de 350-800 nm et chacun desdits accepteurs émet de la lumière dans l'intervalle de longueurs d'onde de 450-1000 nm.

13. Méthode selon la revendication 12, dans laquelle ladite paire donneur-accepteur sont des colorants de type xanthène.

14. Méthode selon la revendication 13, dans laquelle lesdits colorants de type xanthène comprennent des dérivés de fluorescéine et des dérivés de rhodamine.

15. Méthode de séparation de composants dans un mélange multi-composants, dans laquelle chacun des composants d'intérêt est marqué avec des marqueurs différents, dans laquelle lesdits marqueurs sont caractérisés en ce que :

i) chaque marqueur comprend une paire fluorescente donneur-accepteur liée par covalence à des atomes différents d'une chaîne oligonucléotidique avec un transfert d'énergie efficace dudit donneur audit accepteur ;

ii) chacun des marqueurs est une espèce moléculaire unique et chacun absorbe à sensiblement la même longueur d'onde et émet à une longueur d'onde différente ; et

iii) chacun des différents marqueurs a sensiblement la même mobilité dans ladite séparation, ce qui est obtenu en faisant varier l'espacement de ladite paire donneur-accepteur le long de ladite chaîne oligonucléotidique ;

ladite méthode comprenant le fait :

de lier différents marqueurs à différents composants dudit mélange multi-composants ; de séparer lesdits composants en fractions individuelles ; et de détecter chacun desdits composants marqués en irradiant à la longueur d'onde d'absorption desdits donneurs et en détectant la fluorescence de chacun desdits marqueurs.

16. Méthode selon la revendication 15, dans laquelle ladite séparation se fait par électrophorèse.

17. Méthode selon la revendication 15, dans laquelle lesdits fluorophores donneurs sont les mêmes.

18. Méthode selon la revendication 15, dans laquelle ledit donneur absorbe la lumière dans l'intervalle de longueurs d'onde de 350-800 nm et ledit accepteur émet de la lumière dans l'intervalle de longueurs d'onde de 450-1000 nm.

19. Méthode selon la revendication 15, dans laquelle ladite paire donneur-accepteur sont des colorants de type xanthène.

20. Méthode selon la revendication 19, dans laquelle lesdits colorants de type xanthène comprennent des dérivés de fluorescéine et des dérivés de rhodamine.

21. Méthode de séquençage d'un acide nucléique qui emploie des amorces pour copier un acide nucléique monocaténaire et des didésoxynucléotides pour terminer la chaîne à un nucléotide particulier résultant de ladite copie, ladite méthode comprenant le fait :

i) de cloner un fragment d'acide nucléique à séquencer dans un vecteur comprenant une séquence de liaison d'amorce en 5' dudit fragment complémentaire d'une amorce ;

ii) de copier ledit fragment avec une ADN polymérase en présence de ladite amorce, de dNTP et chacun d'une pluralité de didésoxynucléotides dans des récipients de réaction séparés, pour produire des fragments de séquençage d'ADN monocaténaires ;

iii) de séparer le mélange résultant de fragments de séquençage d'ADN monocaténaires et de déterminer la séquence au moyen des bandes présentes sur le gel ;

lesdits fragments de séquençage d'ADN comprenant des amorces qui ont une paire fluorescente donneur-accepteur liée par covalence à des atomes différents d'une chaîne d'atomes formant un squelette avec un transfert d'énergie efficace dudit donneur audit accepteur, dans laquelle chacune desdites amorces est une espèce moléculaire unique et chacune absorbe à sensiblement la même longueur d'onde et émet à une longueur d'onde différente, et dans laquelle chacune des amorces a sensiblement la même mobilité dans ladite séparation, ce qui est obtenu en faisant varier l'espacement et les fluorophores de ladite paire donneur-accepteur le long de ladite chaîne d'acide nucléique.

22. Méthode selon la revendication 21, dans laquelle un des membres de ladite paire fluorescente donneur-accepteur est liée à l'extrémité 5' de ladite amorce.

23. Méthode selon la revendication 21, dans laquelle il y a quatre amorces ayant des paires donneur-accepteur différentes.

24. Méthode selon la revendication 21, dans laquelle ladite paire fluorescente donneur-accepteur n'est pas séparée par plus de 30 nucléotides.

25. Méthode selon la revendication 21, dans laquelle au moins deux desdites paires fluorescentes donneur-accepteur sont des colorants de type xanthène.

26. Méthode selon la revendication 25, dans laquelle lesdits colorants de type xanthène comprennent des dérivés de fluorescéine et des dérivés de rhodamine.

27. Kit destiné à être utilisé dans l'une quelconque des méthodes selon les revendications 10-26 comprenant une combinaison de différents marqueurs fluorescents selon l'une quelconque des revendications 1-9.

Drawing