| (19) |
 |
|
(11) |
EP 0 137 076 A3 |
| (12) |
EUROPEAN PATENT APPLICATION |
| (88) |
Date of publication A3: |
|
21.05.1986 Bulletin 1986/21 |
| (43) |
Date of publication A2: |
|
17.04.1985 Bulletin 1985/16 |
| (22) |
Date of filing: 14.11.1983 |
|
|
| (84) |
Designated Contracting States: |
|
DE FR GB NL |
| (30) |
Priority: |
13.10.1983 JP 19163783
|
| (71) |
Applicants: |
|
- NITTO KAGAKU KOGYO KABUSHIKI KAISHA
()
- HIDEAKI YAMADA
()
|
|
| (72) |
Inventors: |
|
- Hideaki, Yamada
()
- Koitchiro, Ryuno
()
|
|
| |
|
| (54) |
Method for cultivation of pseudomonas bacteria |
(57) Cells of Pseudomonas bacteria having a high nitrile hydratase activity can be obtained
in a high yield by adding to a culture medium at least one amide compound selected
from the group consisting of acrylamide, methacrylamide, crotonamide, and n-butyramide
in the preparation of cells of bacteria having nitrile hydratase activity by cultivating
Pseudomonas bacteria capable of producing nitrile hydratase.