(19)
(11) EP 0 161 901 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
04.03.1987 Bulletin 1987/10

(43) Date of publication A2:
21.11.1985 Bulletin 1985/47

(21) Application number: 85303234

(22) Date of filing: 07.05.1985
(84) Designated Contracting States:
BE CH DE FR GB IT LI NL SE

(30) Priority: 18.05.1984 US 611669
11.02.1985 US 700374

(71) Applicants:
  • NEW ENGLAND MEDICAL CENTER HOSPITALS, INC.
     ()
  • THE TRUSTEES OF TUFTS COLLEGE
     ()
  • WELLESLEY COLLEGE
     ()
  • MASSACHUSETTS INSTITUTE OF TECHNOLOGY
     ()

(72) Inventors:
  • Auron, Philip E.
     ()
  • Webb, Andrew C.
     ()
  • Gehrke, Lee
     ()
  • Dinarello, Charles A.
     ()
  • Rosenwasser, Lanny J.
     ()
  • Rich, Alexander
     ()
  • Wolff, Sheldon M.
     ()

   


(54) Human il-1 cDNA sequences encoding biologically-active human il-1 proteins


(57) Nucleic acids comprise a nucleotide sequence encoding human interleukin-1 (IL-1), and fragments thereof, and the polypeptides and peptides obtained. Truncated human IL-1 cDNA sequences encode biologically-active novel human IL-1 proteins. cDNA synthesised by reverse transcription of poly-(A)RNA isolated from adherent human monocytes stimulated with bacterial endotoxin, is cloned. Truncated human IL-1 cDNA sequences can be obtained by genetic engineering procedures using cloned human IL-1 cDNA as a starting material. Human IL-1 and human IL-1 proteins obtained via cloned truncated human IL-1 cDNA sequences can induce the production of IL-2 by activated T-cells; they also act on B-cells and NK-cells.





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