| (19) |
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(11) |
EP 0 184 438 A3 |
| (12) |
EUROPEAN PATENT APPLICATION |
| (88) |
Date of publication A3: |
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13.01.1988 Bulletin 1988/02 |
| (43) |
Date of publication A2: |
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11.06.1986 Bulletin 1986/24 |
| (22) |
Date of filing: 04.12.1985 |
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| (84) |
Designated Contracting States: |
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AT BE CH DE FR GB IT LI LU NL SE |
| (30) |
Priority: |
05.12.1984 US 678578
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| (71) |
Applicant: ALLELIX INC. |
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() |
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| (72) |
Inventors: |
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- Buxton, Francis Paul
()
- Gwynne, David Ivor
()
- Davies, Roger Wayne
()
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| (54) |
Transforming aspergillus niger, and plasmids for use therein |
(57) Transformants of Aspergillus niger and related Aspergilli, containing foreign DNA
conferring modified properties of expression thereon, are prepared by use of a DNA
vector which contains a selectable marker which is capable of incorporation into the
DNA of the host A. niger cells, but which is not to be found in the A. niger cells
prior to this transformation. The vector also contains other foreign DNA to be incorporated
into the A. niger, for modified expression. The process suitably uses mutants of A.
niger as hosts, the mutants lacking the selectable marker as compared with wild-type
A. niger, and conducts the transformation on spheroplasts of A. niger.