| (57) A process for the manufacture of a polypeptide (I) having the formula:which comprises
steps of:
(a) coupling, successively and in the order of the sequence of the polypeptide (I),
the four protected fragments A, B, C and D or five protected fragments A, B, C, E
and F,
said fragment A by the formula, Leu-Gln-Asp-Ile-Met-Ser-Arg-NH2
said fragment B by the formula, Gln-Leu-Ser-Ala-Arg-Lys-Leu
said fragment C by the formula, Arg-Lys-Val-Leu-Glysaid fragment D by the formula,
Tyr-Ala-Asp-Ala-Ile-Phe-Thr-Asn-Ser-Tyr
said fragment E by the formula, Ile-Phe-Thr-Asn-Ser-Tyr and said fragment F by the
formula, Tyr-Ala-Asp-Ala being represented, respectively, and
(b) eliminating, at the end of sequence, all the protecting groups to provide the
polypeptide (I) which is active on the stimulation of the release of the growth hormone
and thus is very useful as medicine for treatment of growth hormone deficiency disease
and the like.
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