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(11) | EP 0 246 002 A2 |
| (12) | EUROPEAN PATENT APPLICATION |
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| (54) | Alkenamide derivatives |
| (57) The invention concerns novel N-[4(Z)-2-substituted-6-(4-o-hydroxyphenyl-1,3 dioxan-cis-5-yl)
hexenoyllsulphon- amide derivatives of formula I of value as therapeutic agents. Typically
R' is a branched (3-6C)alkyl, trifluoromethyl or phenyl radical as defined hereinafter
and R2 is alkyl, benzyl or phenyl. The invention also discloses salts of the formula I compounds as well as pharmaceutical compositions containing the compounds and processes for the production of the compounds. |
[0001] This invention concerns novel alkenamide derivatives and, more particularly novel N-[4(Z)-6-(4-o-hydroxyphenyl-1,3-dioxan-cis-5-yl)hexenoyl]-sulphonamide derivatives which antagonise one or more of the actions of thromboxane A2 (hereafter referred to as "TXA2") and which are of value as therapeutic agents. The invention also concerns novel pharmaceutical compositions containing one of the novel derivatives and processes and intermediates for use in the manufacture of the novel amide derivatives.
[0002] It is known that TXA2 is a potent aggregator of blood platelets and a powerful vasoconstrictor. TXA2 is also a potent constrictor of bronchial and tracheal smooth muscle. TXA2 may therefore be involved in a wide variety of disease conditions, for example ischaemic heart disease such as myocardial infarction, angina, cerebrovascular disease such as transient cerebral ischaemia, migraine and stroke, peripheral vascular disease such as atherosclerosis, microangiopathy, hypertension and blood clotting defects due to lipid imbalance, and pulmonary disease such as pulmonary embolism, bronchial asthma, bronchitis, pneumonia, dyspnoea and emphysema. Accordingly, compounds which antagonise the actions of TXA2 may be expected to have therapeutic value in the prevention or treatment of any one or more of the above mentioned diseases or any other disease conditions in which it is desirable to antagonise the actions of TXA2.
[0003] In our European patent application, publication number 94239, there is described a series of 4-phenyl-1,3-dioxan-5- ylalkenoic acid derivatives of the formula Z having cis relative stereochemistry at positions 4 and 5 of the dioxane ring and wherein Ra and Rb are variously hydrogen, alkyl, halogenoalkyl, alkenyl and optionally substituted aryl or arylalkyl, Rc is hydroxy, alkoxy or alkanesulphonamido, n is 1 or 2, A is ethylene or vinylene, Y is (2-5C)polymethylene optionally substituted by alkyl and benzene ring B bears one or two optional substituents. We have now discovered (and herein lies the basis of our invention) that particularly useful TXA2 antagonism is also shown by a novel group of amide derivatives of formula Z in which benzene ring B is o-hydroxyphenyl, n is 1, A is cis-vinylene, Y is ethylene and Rc is sulphonamido, as defined below.
[0004] According to the invention there is provided a N-[4(Z)-6-(4-o-hydroxyphenyl-1,3-dioxan-5-yl)hexenoyl]-sulphonamide of the formula I set out hereinafter wherein Rl is trifluoromethyl, (3-5C)branched alkyl or is phenyl optionally bearing a substituent selected from halogeno, cyano, nitro, trifluoromethyl and (l-4C)alkoxy; and R2 is (1-6C)alkyl, benzyl or phenyl, the latter two of which may optionally bear a halogeno, (1-4C)alkyl, (1-4C)alkoxy, trifluromethyl, cyano or nitro substituent; and the substituents at positions 4 and 5 of the dioxane ring in formula I and the substituent Rl have cis-relative stereochemistry; or a pharmaceutically acceptable salt therof.
[0005] It will be appreciated that the compounds of formula I possess asymmetric carbon atoms and may exist and be isolated in racemic and optically active forms. The invention includes both the racemic forms and any optically active form (or mixtures thereof) which is capable of antagonising one or more of the actions of TXA2, it being well known in the art how to prepare individual optical isomers (for example by synthesis from optically active starting materials or resolution of a racemic form) and how to determine the TXA2 antagonist properties using one or more of the standard tests referred to hereafter.
[0006] In the chemical formulae attached hereto, although a particular configuration is shown, this does not neceasarily correspond to the absolute configuration.
[0007] A particular value for Rl when it is (3-5C) branched alkyl is, for example, isopropyl, isobutyl, see-butyl or t-butyl.
[0008] A particular value for an optional substituent which may be present on R1 or R2 when it is phenyl or on R2 when it is benzyl, as defined above, is, for example: fluoro, chloro or bromo, for halogenot methyl or ethyl, for (1-4C)alkyl, and methoxy or ethoxy, for (1-4C)alkoxy.
[0009] Particular pharmaceutically acceptable salts of the sulphonamide derivatives of formula I are, for example, alkali metal and alkaline earth metal salts, such as lithium, sodium, potassium, magnesium and calcium salts; aluminium and ammonium salts; and salts with organic amines and quaternary bases forming physiologically acceptable cations, such as salts with methylamine, dimethylamine, trimethylamine, ethylenediamine, piperidine, morpholine, pyrrolidine, piperazine, ethanolamine, triethanolamine, N-methylglucamine, tetramethylammonium hydroxide and benzyltrimethylammonium hydroxide.
[0010] Specific values for Rl which are of special interest include for example, isopropyl, t-butyl, trifluoromethyl and phenyl optionally bearing a fluoro, chloro, bromo, cyano, nitro, trifluoromethyl or methoxy substituent.
[0011] A preferred value for R1 is isopropyl, t-butyl, trifluoromethyl, 2-chlorophenyl, 2-cyanophenyl, 3-(trifluoromethyl)phenyl, 4-chlorophenyl, 4-cyanophenyl or 4-nitrophenyl.
[0013] Specific compounds of formula I of particular interest are set out in the accompanying Examples.
[0014] The compounds of formula I may be manufactured by conventional procedures of organic chemistry well known in the art for the manufacture of structurally analogous compounds. Such procedures are provided as a further aspect of the invention and are illustrated by the following processes in which R1 and R2 have any of the meanings hereinabove:-
(a) An aldehyde of the formula II is reacted with a Wittig reagent of the formulas
wherein R'is (I-6C)alkyl or aryl (especially phenyl) and M+ is a cation, for example an alkali metal cation, such as the lithium, sodium or potassium cation.
[0015] The process in general produces compounds of formula I in which the substituents adjacent to the double bond have predominantly the required cis-relative stereochemistry i.e. the 'Z' isomer. Any unwanted 'E' isomer may be removed by standard purification procedures such as crystallisation or chromatography.
[0016] The process is conveniently performed in a suitable solvent or diluent, for example an aromatic solvent such as benzene, toluene or chlorobenzene, an ether such as 1,2-dimethoxyethane, t-butyl methyl ether, dibutyl ether or tetrahydrofuran, in dimethyl sulphoxide or tetramethylene sulphone, or in a mixture of one or more such solvents or diluents. The process is generally performed at a temperature in the range, for example, -80°C to 40°C, but is conveniently performed at or near room temperature, that is in the range 0 to 350C.
(b) A phenol derivative of the formula III, wherein R" is a suitable protecting group, for example (1-6C)alkyl (such as methyl or ethyl), acyl (such as acetyl, benzoyl, methanesulphonyl or p-toluenesulphonyl), allyl, tetrahydropyran-2-yl or trimethylsilyl, is deprotected.
[0017] The precise deprotection conditions used depend on the nature of the protecting group R". Thus, for example, when it is methyl or ethyl the deprotection may be carried out by heating with sodium thioethoxide in a suitable solvent (such as N,N-dimethylformamide or l,3-dimethyl-3,4,5,6-tetrahydro-2(lH)-pyrimidinone)at a temperature in the range, for example, 60-1600C. Alternatively, an ethyl or methyl protecting group may be removed by reaction with lithium diphenylphosphide in a suitable solvent (such as tetrahydrofuran or t-butyl methyl ether) at a temperature in the range, for example, 0-60°C. When the protecting group is acyl it may be removed, for example, by hydrolysis in the presence of a base (such as sodium or potassium hydroxide) in a suitable aqueous solvent [such as an aqueous (1-4C)alkanol] at a temperature in the range, for example, 0-60°C. When the protecting group is allyl or tetrahydropyran-2-yl it may be removed, for example, by treatment with strong acid such as trifluoroacetic acid and when it is trimethylsilyl, it may be removed, for example, by reaction with aqueous tetrabutylammonium fluoride or sodium fluoride, using a conventional procedure.
(c) For a compound of the formula I wherein Rl is other than trifluoromethyl, an erythro-diol derivative of the formula IV, wherein one of.Ql and Q2 is hydrogen and the other is hydrogen or a group of the formula -CRaRb.OH (wherein Ra and Rb are the same or different (1-4C) alkyl), is reacted with an aldehyde of the formula R6.CO.H, in which R6 has the same meaning as Rl apart from trifluoromethyl, or with an acetal, hemiacetal or hydrate thereof.
[0018] The aldehyde [or its hydrate, or its acetal or hemiacetal with a (1-4C)alkanol (such as methanol or ethanol)] is generally used in excess.
[0019] The reaction is generally performed in the presence of an acid catalyst, such as hydrogen chloride, hydrogen bromide, sulphuric acid, phosphoric acid, methanesulphonic acid, p-toluenesulphonic acid, or an acidic resin, conveniently in the presence of a suitable solvent or diluent, such as toluene, xylene or an ether, for example tetrahydrofuran, dibutyl ether, methyl t-butyl ether or 1,2-dimethoxyethane, and at temperature in the range, for example 0 to 80°C.
[0020] Those starting materials of formula IV wherein Ql and Q2 are both hydrogen may be obtained, for example, by mild acid catalysed hydrolysis or alcoholysis of the dioxane ring of a compound of formula V wherein Ra and Rb are both alkyl, such as methyl or ethyl. The hydrolysis or alcoholysis will normally be carried out a temperature in the range 10 to 80°C using an aqueous mineral acid such as hydrochloric acid, in an alkanol (such as ethanol or 2-propanol) or an ether (such as tetrahydrofuran), as solvent.
[0021] Those starting materials of formula IV wherein one of Ql and Q2 is hydrogen and the other is a group of the formula - CRaRb.OH are intermediates in the above-mentioned formation of the starting materials of formula IV wherein Ql and Q2 are both hydrogen. However, said intermediates are not normally isolated or characterised. Accordingly, the invention also provides as procedure (d) a modification of process (c) which comprises reacting a compound of formula V wherein one of Ra and Rb is hydrogen, methyl or ethyl and the other is methyl or ethyl, with an excess of an aldehyde of the formula R6.CO.H or an acetal, hemiacetal or hydrate thereof, in the presence of an acid- catalyst(such as one of those given above), conveniently at a temperature in the range, for example, 10 to 80°C and optionally in the presence of a suitable solvent or diluent (such as one of those given above).
[0022] The starting materials for use in the above processes may be made by general procedures of organic chemistry, known for the preparation of structurally related compounds, for example by analogy with those procedures disclosed in European patent application, publication No. 94239.
[0023] The protected phenol derivatives of formula III may be made, for example, by using an analogous procedure to process (a) above, using an aldehyde analogous to formula II but wherein the phenol group has been protected with the group R". The starting materials of formula V may be obtained, for example, using analogous procedures to those described in European patent application, publication number 94239.
[0024] The necessary Wittig reagents may be obtained, for example, by conventional procedures, for example by treating the corresponding phosphonium halides with a strong base, such as sodium hydride, lithium diisopropylamide, potassium t-butoxide or butyllithium. They are generally formed in situ just prior to carrying out the condensation process (a) above.
[0025] The necessary starting materials of formula III may conveniently be, for example, obtained by reacting the corresponding protected acid of formula VI with a sulphonamide of the formula H2N.S02R2 and a suitable dehydrating agent, for example N,N'-dicyclohexylcarbodiimide, optionally together with an organic base, for example 4-(dimethylamino)pyridine, in the presence of a suitable solvent or diluent, for example methylene chloride at a temperature in the range, 10-50°C, but preferably at or near room temperature. Alternatively, a reactive derivative of the acid of formula VI, for example an acid halide (such as the acid chloride), may be reacted with an alkali metal salt (such as the sodium salt) of the appropriate sulphonamide, conveniently at or near room temperature and in a suitable solvent or diluent, for example an ether, N,N-dimethylformamide or methylene chloride.
[0026] When a salt of a compound of formula I is required, it may be obtained by reaction with the appropriate base affording a physiologically acceptable cation, or by any other conventional procedure.
[0027] Further, when an optically active form of a compound of formula I is required, one of the aforesaid processes is carried out using an optically active starting material. Alternatively, the racemic form of a compound of formula I may be reacted with an optically active form of a suitable organic base, for example ephedrine, N,N,N-trimethyl(l-phenylethyl)ammonium hydroxide or I-phenylethylamine, followed by conventional separation of the diastereoisomeric mixture of salts thus obtained, for example by fractional crystallisation from a suitable solvent, for example a (1-4C)alkanol, whereafter the optically active form of said compound of formula I may be liberated by treatment with acid using a conventional procedure for example using an aqueous mineral acid such as dilute hydrochloric acid.
[0028] Many of the intermediates defined herein are novel, for example the phenol derivatives of formula III, and are provided as further separate features of the invention.
[0029] As stated earlier, the compounds of formula I are antagonists of one or more of the actions of TXA2, for example certain of its actions on blood platelets, the vasculature and/or the lung. The antagonism may be demonstrated in one or other of the following standard tests:-
(a) The rabbit aortal strip model devised by Piper and Vane (Nature, 1969, 223, 29-35) or the rat aortal strip model developed by Kennedy et alia (Prostaglandins, 1982, 24, 667-689) using as agonist the TXA2 mimetic agent known as U46619 (described by R L Jones et alia in "Chemistry, Biochemistry and Pharmacological Activity of Prostanoids" edited by S K Roberts and F Scheinmann, at page 211; Pergamon Press, 1979) may be used as the agonist; and
(b) a blood platelet aggregation test based on that described by Born (Nature, 1962, 194, 927-929) and involving:
(i) aggregating human, citrated, platelet-rich plasma by addition of the TXA2 mimetic agent U46619 so that a dose-response curve is generated;
(ii) generating a dose-response curve for U46619 stimulated platelet aggregation in the presence of increasing amounts of test compound (generally in the range 10-5M to 1010M); and
(iii) calculating a KB value indicating potency of TXA2 antagonism for the test compound, averaged over several concentrations, from the calculated 50X response value for U46619 aggregation in the presence and absence of test compound; and
(c) a bronchoconstriction test involving measuring the inhibition by a test compound of the bronchoconstriction induced in the Konzett-Rossler, anaesthetised guinea-pig model (as modified by Collier and James, Brit. J. Pharmacol., 1967, 30, 283-307) by intravenous administration of the TXA2 mimetic agent, U46619 and involving i
(i) obtaining a cumulative dose-response curve to U46619 induced bronchoconstriction by intravenous administration of constant volumes of increasing concentrations of U46619 (0.2-4 ug/kg) in physiological saline solution and expressing bronchoconstriction as the maximum of that theoretically obtainable with no air flow to the test animal;
(ii) generating a cumulative dose-response curve to U46619 induced bronchoconstriction at 30 minute intervals for 3 hours after oral dosing of test compound; and
(iii) calculating a dose-ratio for the test compound (that is the ratio of concentration of U46619 required to cause 50% bronchoconstriction in the presence and absence of test compound) indicating the potency of TXA2 antagonism.
[0030] The antagonism of the effects of TXA2 on the vasculature may be demonstrated, for example in rats in the following manner:-
(d) Male rats (Alderley Park strain) are anaesthetised with sodium pentobarbital and blood pressure is monitored at the carotid artery. The TXA2 mimetic agent U46619 is administered intravenously at 5 ug/kg via the jugular vein to produce 20-30 mm/Hg (2640-3970 pascal) increase in systolic blood pressure. The process is repeated twice to ensure adequacy of response. A test compound is then administered either intravenously (via the jugular vein) or orally (via a cannula) directly into the stomach and the animal challenged with U46619, five minutes after dosing with test compound and then successively every ten minutes until the hypertensive effect of U46619 is no longer blocked.
[0031] Further, the antagonism of the effects of TXA2 in vivo may be demonstrated, for example, by assessing the effects of a test compound on the aggregation of blood platelets obtained after administration of test compound to a test animal, such as a rabbit, rat, guinea pig or dog, using standard procedures similar to that described in (a) above. However, when the aggregation of dog platelets is being studied it is necessary to use a predetermined, threshold concentration of the platelet aggregation agent adenosine diphosphate (about 0.4-1.2 x 10-6M) together with the TXA2 mimetic agent, U46619.
[0032] By way of illustration, the compound described in Example 3 hereafter possesses a KB of 1.05 x 10-8M in procedure (b) above.
[0033] In general, other compounds of formula I show the following levels of TXA2 antagonist properties in one or more of the above mentioned tests e.g. test (a) pA2 > 6.0; test (b) KB < 1.0 x 10-6M; test (c) dose ratio > 5, 2 hours after oral dosing at 10 mg/kg or less and/or test (d), significant inhibition of U46619 induced hypertension for at least 1 hour following oral dosing at 25 mg/kg or less, without any overt toxicity in tests (c) or (d).
[0034] As stated previously, the compounds of formula I may be used in the therapy or prevention of diseases or adverse conditions in warm-blooded animals in which it is desirable to antagonise one or more of the actions of TXA . In general, a compound of formula I will be administered for this purpose by an oral, rectal, intravenous, subcutaneous, intramuscular or inhalation route, so that a dose in the range, for example.0.01-5 mg/kg body weight, will be given up to four times per day, varying with the route of administration, the severity of the condition and the size and age of the patient under treatment.
[0035] The compounds of formula I will generally be used in the form of a pharmaceutical composition comprising a compound of formula I, or a pharmaceutically acceptable salt thereof, as defined hereinabove, together with a pharmaceutically acceptable diluent or carrier. Such a composition is provided as a further feature of the invention and may be in a variety of dosage forms. For example, it may be in the form of tablets, capsules, solutions or suspensions for oral administration; in the form of a suppository for rectal administration; in the form of a sterile solution or suspension for administration by intravenous or intramuscular injection; in the form of an aerosol or a nebuliser solution or suspension, for administration by inhalation; and in the form of a powder, together with pharmaceutically acceptable inert solid diluents such as lactose, for administration by insufflation.
[0036] The pharmaceutical compositions may be obtain by conventional procedures using pharmaceutically acceptable diluents and carriers well known in the art. Tablets and capsules for oral administration may conveniently be formed with an enteric coating, for example comprising cellulose acetate phthalate, to minimise contact of the active ingredient of formula I with stomach acids.
[0037] The pharmaceutical compositions of the invention may also contain one or more agents known to be of value in diseases or conditions intended to be treated; for example a known platelet aggregation inhibitor, hypolipidemic agent, antihypertensive agent, beta- adrenergic blocker or a vasodilator may usefully also be present in a pharmaceutical composition of the invention for use in treating a heart or vascular disease or condition. Similarly, by way of example, an anti-histamine, steroid (such as beclomethasone dipropionate), sodium cromoglycate, phosphodiesterase inhibitor or a beta-adrenergic stimulant may usefully also be present in a pharmaceutical composition of the invention for use in treating a pulmonary disease or condition. A composition according to the invention may also advantageously contain an inhibitor of thromboxane A2 synthetase, for example dazoxiben or furegrelate (U63557).
[0038] In addition to their use in therapeutic medicine, the compounds of formula I are also useful as pharmacological tools in the development and standardisation of test systems for the evaluation of the effects of TXA 2 in laboratory animals such as cats, dogs, rabbits, monkeys, rats and mice, as part of the search for new therapeutic agents. The compounds of formula I may also be used because of their TXA 2 antagonist properties in helping to maintain the viability of blood and blood vessels in warm-blooded animals (or parts thereof) under-going artificial extracorporeal circulation, for example during limb or organ transplants. When used for this purpose a compound of the formula I, or a physiologically acceptable salt thereof, will generally be administered so that a steady state concentration in the range, for example, 0.1 to 10 mg. per litre is achieved in the blood.
[0039] The invention will now be illustrated in the following non-limiting Examples in which, unless otherwise stateds-
(i) evaporations were carried out by rotary evaporation in vacuo;
(ii) operations were carried out at room temperature, that is in the range 18-260C and under an atmosphere of an inert gas such as argon;
(iii) flash column chromatography was performed on Merck Kieselgel (Art. 9385) obtained from E.Merck, Darmstadt, W.Germany;
(iv) yields are given for illustration only and are not necessarily the maximum attainable;
(v) proton NMR spectra were normally determined at 90 or 200 MHz in CDC13 using tetramethylsilane (THS) as an internal standard, and are expressed as chemical shifts (delta values) in parts per million relative to TMS using conventional abbreviations for designation of major peaks: s, singlet; m, multiplet, t, triplet, br, broad d,doublet,
(vi) all end-products were isolated as racemates.
Example 1
[0040] o-Chlorobenzaldehyde (90 mg) and p-toluenesulphonic acid (2 mg) were added to a stirred solution of N- - ethanesulphonyl-4(Z)-6-(4-o-hydroxyghenyl-2,2-dimethyl-1,3-dioxan-cis-5-yl)hexenamide (A) (209 mg) in toluene (3 ml). The reaction mixture was stirred for 1.5 hours and then purified by flash chromatography on silica, using 50,50:1 (by volume) hexane/ethyl acetate/ acetic acid as eluant. There was thus obtained N-ethanesulphonyl-4(Z)-6-([2,4,5-cis]-2-o-chlorophenyl-4-o-hydroxyphenyl-1,3-dioxan-5-yl)hexenamide, as an oil (95 mg, 38%); NMR; 1.25 (3H, t), 1.82 (1H, m), 2.00 (1H, m), 2.30 (4H, m), 2.75 (1H, m), 3.40 (2H, q), 4.2 (2H, m) 5.41 (3H, m), 6.05 (lH, s), 7.15 (7H, m) and 7.72 (1H, m).
[0041] The starting sulphonamide A was obtained as follows:-
(i) N,N'-Dicyclohexylcarbodiimide (824 mg) was added to a stirred solution of 4(Z)-6-(4-o-methoxyphenyl-2,2-dimethyl-1,3-dioxan-cie-5yl)hexenoic acid (1.336 g), 4-(dimethylamino)pyridine (508 mg) and ethanesulphonamide (436 mg) in dichloromethane (45 ml). The solid which had formed was collected by filtration. The filtrate was evaporated. The oil which was left was purified by flash chromatography on silica, using 80:20:2 (by volume) toluene/ethyl acetate/acetic acid as eluant, to give N-ethanesulphonyl-4(Z)-6-(4-o-methoxyphenyl-2,2-dimethyl-1,3-dioaan-cis-5-yl)-hexenamide (B) as a colourless oil (1.26 g, 74%), NMR: 1.37 (3H, t), 1.60 (6H, s) 1.65 (1H, m), 1.85 (1H, m), 2.55 (5H, m), 3.45 (2H, q), 3.75 (1H, dd), 3.85 (3H, s), 4.2 (1H, dt), 5.32 (3H, m), 6.92 (2H, m), 7.26 (1H, m) and 7.55 (1H, m).
(ii) To a solution of B (1.114 g) in 1,3-dimethyl-3,4, 5,6-tetrahydro-2(lH)-pyrimidinone (DMPU) (27 ml) was added sodium hydride (778 mg) 50X w/w dispersion in mineral oil) at 0-5°C. After 3 minutes, ethanethiol (1.004 ml) was added dropwide during 5 minutes. The mixture was maintained at 0-5°C for 5 minutes and then heated to 95°C for 6 hours. The cooled mixture was diluted with water (60 ml) and extracted with dichloromethane (3 x 50 ml). The aqueous phase was acidified to pH4 with acetic acid and extracted with ether (3 x 50 ml). The extracts were washed with saturated brine (2 x 50 ml), dried (MgS04) and evaporated. The oil obtained was purified by flash chromatography on silica, using 50.50:1 (by volume) hexane/ethyl acetate/acetic acid as eluant, to give N-ethanesulphonyl-4(Z)-6-(4-o-hydroxyphenyl-2,2-dimethyl-1,3-dioxan-cis-5-yl)hexenamide (A), as a colourless oil (870 mg 78%), NMR; 1.38 (3H, t), 1.60 (6H, a), 1.65 (1H, m), 1.85 (1H, m), 2.35 (4H, m), 2.70 (1H, m) 3.45 (1H, q), 3.82 (1H, dd), 4.18 (1H, dt), 5.38 (3H, m), 6.88 (3H, m), 7.15 (1H, m), 8.1 (1H, m) and 8.3 (1H, m).
[0042] The starting hexenoic acid was itself obtained as follows:-
Potassium t-butoxide (12.3 g) was added over 2 minutes to a stirred suspension of (3-carboxypropyl)-triphenylphosphonium bromide (23.6 g) in tetrahydrofuran (THF) (230 ml) at 0-5°C. The mixture was stirred at ambient temperature for 30 minutes and cooled to 0°C, before the addition of (6-o-methoxyphenyl-2,2-dimethyl-1,3-dioxan-cis-5-yl)acetaldehyde (5.9 g) during 5 minutes. The mixture was stirred for 45 minutes and water (50 ml) added. The solvent was removed by evaporation. The residue was dissolved in water (250 ml). The solution was washed with ethyl acetate (3 x 100 ml.) and then acidified to pH4 with acetic acid. The liberated oil was extracted with ethyl acetate (3 x 100 ml). These extracts were washed with saturated brine (2 x 100 ml), dried (MgSO4) and evaporated to give an oil. The oil was purified by flash column chromatography on silica, eluting with 80:20:1 (by volume) toluene/ethyl acetate/acetic acid, to give 4(Z)-6-(4-o-methoxyphenyl-2,2-dimethyl-1,3-dioxan-cis-5-yl)hexenoic acid as a colourless solid (6.0 g, 82X), m.p. 92-96°C; (m.p. 99-101°C after recrystallisation from ethyl acetate/hexane) ; NMR: 1.65 (8H,m), 2.35 (5H,m), 3.85 (5H,m), 5.28 (3H,m) and 7.1 (4H, m). Example 2
[0043] Sodium hydride (205 mg, 50% w/w dipersion in mineral oil) was added to a stirred suspension of N-ethanesulphonyl-4(Z)-6-([2,4,5-cis-]-2-p-cyanophenyl-4 -o-methoxyphenyl-1,3-dioxan-5-yl)hexenamide (C) (355 mg) in DMPU (8 ml) at 0-5°C. After 5 minutes, ethanethiol (260 mg) was added during 2 minutes. The mixture was maintained at 0-5° for 10 minutes and then was heated to 90-95° for 4 hours. The cooled reaction mixture was diluted with water (20 ml) and extracted with dichloromethane (3 x 30 ml). The aqueous phase was acidified to pH4 with acetic acid and extracted with ether (4 x 50 al). The extracts were washed with saturated brine (2 x 30 ml), dried (MgSO4) and evaporated. The oil obtained was purified by flash chromatography on silica, using 97.5:2.5 v/v dichlorooethane/ethanol as eluant, to give N-ethanesulphonyl-4(Z)-6-([2,4,5-cis]-2-p-cyanophenyl-4-o-hydroxyphenyl-1,3-dioxan-5-yl)hexenamide, as a colourless solid (215 mg, 63%), m.p. 63-6°C; NMR 1.35 (3H, t), 1.90 (2H, m), 2.28 (4H, s), 2.60 (1H, m), 3.42 (2H. q), 4.16 (1H, d), 4.25 (1H, d), 5.40 (3H, m), 5.78 (1H, s), 6.90 (1H, m), 7.15 (2H, m), 7.70 (4H, q) and 7.96 (1H, s); m/e: 484 (M+).
[0044] The starting sulphonamide C was obtained as follows:-
(i) A solution of 4(Z)-6-(4-o-methoxyphenyl-2,2-dimethyl-1,3-dioxan-cis-5-yl)hexenoic acid (668 mg), p-cyanobenaaldehyde (300 ag) and p-toluenesulphonic acid (5 mg) in toluene (9 ml) was heated at 105°C for 1 hour. The cooled reaction mixture was purified by flash chromatography on silica, using 95,5 v/v dichloromethane/ethanol as eluant, to give 4(Z)-6-((2,4,5-cis]-2-p-cyanophenyl-4-o-methoxyphenyl-1,3-dioxan-5-yl)hexenoic acid, as a colourless solid (470 mg, 57%), m.p. 110-113°C; NMR; 1.65 (1H, m), 1.98 (1H, m), 2.25 (4H, m), 2.45 (1H, m), 3.82 (3H, s), 4.12 (1H, dt), 4.22 (1H, dt), 5.30 (3H, m), 5.78 (1H, s), 6.90 (2H, m), 7.25 (1H, m), 7.42 (1H, m) and 7.69 (4H, s).
(ii) 4-(Dimethylamino)pyridine (130 mg), ethane sulphonamide (116 mg) and N,N-dicyclohexylcarbodiimide (218 mg) were added to a stirred solution of D (432 mg) in dichloromethane (12 ml). Stirring was continued for 2 hours. The reaction mixture was then cooled to 0°C and ether (30 ml) was added. The solid which formed was separated by filtration. The filtrate was evaporated and the residue was purified by flash chromatography on silica, using 97.5; 2.5 v/v dichloromethane/ethanol as eluant, to give N-ethanesulphonyl-4(Z)-6(]2,4,5-cis]-2- p-cyanophenyl-4-o-methoxyphenyl-1,3-dioxan-5-yl)hexenamide (C), as a colourless solid (385 mg, 73%), m.p. 154-156°C; NMR; 1.33 (3H, t), 165 (1H, m), 2.02 (1H, m), 2.25 (4H, m), 2.52 (lH, m), 3.37 (2H, q), 3.83 (3H. s) 4.2 (2H, s), 5.35 (3H, m), 5.79 (1H, s), 7.17 (4H, m) and 7.70 (4H, s).
Example 3
[0045] Ethanethiol (0.67 ml) was added over 15 minutes to a stirred suspension of sodium hydride (432 mg, 50% w/w dispersion in oil) in DHPU (15 ml) at 4°C, maintained under an argon atmosphere. After 30 minutes, the temperature was raised to 85°C and N-methanesulphonyl-4(Z)-6-([2,4,5-cis]-2-t-butyl-4-o-methoxyphenyl-1,3-dioxan-5-yl)hexenamide (659 mg) was added. The mixture was stirred for 3 hours, then cooled to 10°C and poured into an ice-water mixture (60 ml). The aqueous mixture was washed with dichloromethane (2 x 30 ml). The aqueous phase was acidified with acetic acid and extracted with ether (3 x 30 ml). These extracts were washed with water (2 x 20 ml) and saturated brine (2 x 20 ml), then dried (MgSO4) and evaporated. The oil obtained was purified by flash chromatography, using 70:30:1 (by volume) hexane/ethyl acetate/acetic acid as eluant, to give an oil which crystallised on standing, to give N-methanesulphonyl-4(Z)-6-([2,4,5-cis]-2-t-butyl-4-o-hydroxyphenyl-1,3-dioxan-5-yl)hexenamide (318 mg), m.p. 142-143.5°C, NMR: 1.01 (9H, s), 1.67 (1H, m), 1.89 (lH, m), 2.32 (4H, m), 2.59 (1H, m), 3.28 (3H, s), 3.90 (lH, dm J=11Hz), 4.10 (1H, dd J=11,1Hz), 4.38 (1H, s), 5.22 (1H, d J-2Hz), 5.38 (2H, m) ; 6.89 (3H, m), 7.18 (1H, m); m/e 425 (M+).
[0046] The starting material was obtained as follows:-
(i) A suspension 4(Z)-6-(4-o-methoxyphenyl-2,2-dimethyl-1,3-dioxan-cis-5-yl)hexenoic acid (1.88 g) in 2,2-dimethylpropionaldehyde (5 ml) was treated with p-toluenesulphonic acid (5 mg). The mixture was then stirred for 18 hours and then ether (50 ml) was added. The mixture was extracted with 0.5 H potassium hydroxide (4 x 25 ml). The combined aqueous extracts were acidified to pH 5 (acetic acid) and then extracted with ether (3 x 50 ml). The combined extracts were washed successively with water (2 x 50 ml) and saturated brine (50 ml), then dried (MgS04) and evaporated. The residue was purified by MPLC eluting with hexane/ethyl acetate/ acetic acid (85:15:1 v/v). A clear oil was obtained which crystallised on standing to give 4-(Z)-6-([2,4,5-cis]-2-t-butyl-4-o-methoxyphenyl-1,3-dioxan-5-yl)hexenoic acid (2.276 g), a.p. 74-77°C; NMR, 0.98 (9H, s), 1.51 (1H, m), 1.80 (1H, m), 2.27 (4H, m), 2.45 (1H, m), 3.80 (3H,s), 3.85 (lH,dm J=11Hz), 4.02 (1H, dd, J = 11, 1 Hz), 4.37 (1H, s), 5.13 (1H, d J = 2 Hz), 5.27 (2H, m), 6.83 (1H, dd J = 7, 1 Hz), 6.97 (1H, td J = 7, 1 Hz), 7.22 (1H, td J = 7, 1.5 Hz), 7.45 (1H, dd J = 7, 1.5 Hz). (ii) N,N'-Dicyclohexylcarbodiimide (1.08 g) was added to a solution of 4(Z)-6-([2,4,5-cis]-2-t-butyl-4-o-methoxyphenyl-1,3-dioxan-5-yl)hexenoic acid (1.81 g), 4-(dimethylamino)pyridine (0.61 g) and methanesulphonamide (0.48 g) in dichloromethane (20 ml). The mixture was stirred for 1 hour at 4°C then for 18 hours at ambient temperature. The precipitated N,N'- dicyclohexylurea was removed by filtration, and washed with dichloromethane. The filtrate and washings were extracted with 0.1 M sodium hydroxide (50 ml). The basic extract was acidified with acetic acid and extracted with ether (3 x 30 ml). These extracts were washed with water (3 x 20 ml) and saturated brine (1 x 20 ml), then dried (MgS04) and evaporated. The oil obtained was purified by flash chromatography, using 75:25:1 (by volume) hexane/ethyl acetate/acetic acid as eluant, to give an oil, which crystallised on standing, yielding N-methylsulphonyl-4(Z)-6-([2,4,5-cis]-2-t-butyl-4-o-methoxyphenyl-1,3-dioxan-5-yl)hexenamide (1.83 g), m.p. 146-148°C; NMR, 1.01 (9H, s), 1.49 (1H, m), 1.87 (lH, m), 2.40 (5H, m), 3.27 (3H, s), 3.81 (3H, s), 3.90 (1H, dm J- llHz), 4.05 (lH, dd J=11,1 Hz), 4.39 (1H, s), 5.18 (lH, d J-2Hz), 5.35 (2H, m), 6.86 (1H, dd J=7,1 Hz), 6.98 (1H, dt J-7,1 Hz), 7.26 (1H, dt J-7, 1.5 Hz), 7.45 (1H, dd J-7, 1.5 Hz), m/e 439 (M+).
Example 4
[0047] An illustrative dosage form of a composition of the invention is provided by the following capsule formulations-
The capsules may conveniently be of hard gelatine and are filled in conventional manner. Compound X
is a compound of formula I or a salt thereof as defined hereinbefore, for example the compound of Example 1, 2 or 3 or a pharmaceutically acceptable salt thereof.
(a) an aldehyde of the formula II is reacted with a Wittig reagent of the formula:
R'3P=CH.(CH2)2.CO.N-.SO2R2 M+ wherein R' is (l-6C)alkyl or aryl and M+ is a suitable cation;
(b) a phenol derivative of the formula III, wherein R" is a suitable protecting group is deprotected;
(c) an erythro-diol derivative of the formula IV, wherein one of Ql and Q2 is hydrogen and the other is hydrogen or a group of the formula -CRaRb.OH wherein Ra and Rb are the same or different (1-4C)alkyl, is reacted with an aldehyde of the formula Rl.CO.H, or with an acetal, hemiacetal or hydrate thereof; or
(d) a compound of the formula V wherein one of Ra and Rb is hydrogen, methyl or ethyl and the other is methyl or ethyl is reacted with an aldehyde of the formula R1.CO.H, or with an acetal, hemiacetal or hydrate thereof in the presence of an acid catalyst;
whereafter, when an optically active form of a compound of formula I is required, either one of the procedures (a)-(d) above is carried out using an optically active form of the appropriate starting material, or the racemic form is resolved using an appropriate optically active base; and when a pharmaceutically acceptable salt is required, the free acidic form of the compound of the formula I is reacted with an appropriate base affording a physiologically acceptable cation; and wherein R1 and R2 have the meanings set out in any of claims 1 to 5; and the formulae II, III, IV and V are set out hereinafter.
(a) an aldehyde of the formula II is reacted with a Wittig reagent of the formula:
R'3P=CH.(CH2)2.CO.N-.SO2R2 M+ wherein R' is (1-6C)alkyl or aryl and M+ is a suitable cation;
(b) a phenol derivative of the formula III, wherein R" is a suitable protecting group is deprotected;
(c) an erythro-diol derivative of the formula IV, wherein one of Ql and Q2 is hydrogen and the other is hydrogen or a group of the formula -CRaRb.OH wherein Ra and Rb are the same or different (1-4C)alkyl, is reacted with an aldehyde of the formula Rl.CO.H, or with an acetal, hemiacetal or hydrate thereof; or
(d) a compound of the formula V wherein one of Ra and Rb is hydrogen, methyl or ethyl and the other is methyl or ethyl is reacted with an aldehyde of the formula R1.CO.H, or with an acetal, hemiacetal or hydrate thereof in the presence of an acid catalyst;
whereafter, when an optically active form of a compound of formula I is required, either one of the procedures (a)-(d) above is carried out using an optically active form of the appropriate starting material, or the racemic form is resolved using an appropriate optically active base; and when a pharmaceutically acceptable salt is required, the free acidic form of the compound of the formula I is reacted with an appropriate base affording a physiologically acceptable cation; and
wherein Rl and R2 have the meanings defined above; and the formulae II, III, IV and V are set out hereinafter.
(a) an aldehyde of the formula II is reacted with a Wittig reagent of the formulas
R'3P'=CH.(CH2)2.CO.N-.SO2R2 M+ wherein R' is (l-6C)alkyl or aryl and M+ is a suitable cation;
(b) a phenol derivative of the formula III, wherein R" is a suitable protecting group is deprotected;
(c) an erythro-diol derivative of the formula IV, wherein one of Ql and Q2 is hydrogen and the other is hydrogen or a group of the formula -CRaRb.OH wherein Ra and Rb are the same or different (1-4C)alkyl, is reacted with an aldehyde of the formula R1.CO.H, or with an acetal, hemiacetal or hydrate thereof; or
(d) a compound of the formula V wherein one of Ra and Rb is hydrogen, methyl or ethyl and the other is methyl or ethyl is reacted with an aldehyde of the formula R1.CO.H, or with an acetal, hemiacetal or hydrate thereof in the presence of an acid catalyst,
whereafter, when an optically active form of a compound of formula I is required, either one of the procedures (a)-(d) above is carried out using an optically active form of the appropriate starting material, or the racemic form is resolved using an appropriate optically active base; and when a pharmaceutically acceptable salt is required, the free acidic form of the compound of the formula I is reacted with an appropriate base affording a physiologically acceptable cation; and
wherein Rl and R2 have the meanings defined above; and the formulae II, III, IV and V are set out hereinafter.
2-yl or trimethylsilyl or a salt thereof.