(19)
(11) EP 0 310 887 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
18.07.1990 Bulletin 1990/29

(43) Date of publication A2:
12.04.1989 Bulletin 1989/15

(21) Application number: 88115674.9

(22) Date of filing: 23.09.1988
(51) International Patent Classification (IPC)4: C07K 7/10, C12P 21/02, A61K 37/02
(84) Designated Contracting States:
AT BE CH DE ES FR GB GR IT LI LU NL SE

(30) Priority: 09.10.1987 JP 255381/87

(71) Applicants:
  • Takeda Chemical Industries, Ltd.
    Chuo-ku, Osaka (JP)
  • Director-General of the Agency of Industrial Science and Technology
    Chiyoda-ku Tokyo 100 (JP)

(72) Inventors:
  • Masaki, Tomoh
    Tsukuba-shi Ibaraki 305 (JP)
  • Goto, Katsutoshi
    Tsukuba-shi Ibaraki 305 (JP)
  • Kimura, Sadao
    Tsukuba-shi Ibaraki 305 (JP)
  • Mitsui, Youji
    Matsudo Chiba 270 (JP)
  • Yazaki, Yoshio
    Bunkyo-ku Tokyo 112 (JP)
  • Yanagisawa, Masashi
    Tsukuba-shi Ibaraki 305 (JP)
  • Kurihara, Hiroki
    Koto-ku Tokyo 136 (JP)

(74) Representative: von Kreisler, Alek, Dipl.-Chem. et al
Patentanwälte von Kreisler-Selting-Werner Postfach 10 22 41
50462 Köln
50462 Köln (DE)


(56) References cited: : 
   
       


    (54) Vasoconstrictor peptide


    (57) Disclosed is a vasoconstrictor peptide called Endothelin having a molecular weight of 2,500 ± 300. Endothelin contains 21 amino acid residues, wherein four cysteines form two sets of S-S bond.
    In order to obtain the vasoconstrictor factors of the present invention, it is preferable to culture the endothelial cells collected from the vascular inner walls in a serum-free medium.
    The vasoconstrictor peptides of the present invention can be utilized as hypotension therapeutic agents or local vasoconstrictors to animals including humans.





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