(19)

(11)

EP 0 318 502 A1

(12)

(43) Date of publication: 07.06.1989 Bulletin 1989/23 (21) Application number: 87905388.0 (22) Date of filing: 06.08.1987

(51) International Patent Classification (IPC):  C12N 15/ 09( . ) C12Q 1/ 70( . ) C12Q 1/ 68( . ) (86) International application number: PCT/US1987/001889 (87) International publication number: WO 1988/001301 (25.02.1988 Gazette 1988/05)

(84) Designated Contracting States: AT BE CH DE FR GB IT LI LU NL SE (30) Priority: 13.08.1986 US 19860895946 (71) Applicant: THE GENERAL HOSPITAL CORPORATION Boston, MA 02114 (US)	(72) Inventors: GATTONI-CELLI, Sebastiano Boston, MA 02114 (US) ISSELBACHER, Kurt, J. Newton Centre, MA 02159 (US) (74) Representative: Sheard, Andrew Gregory, et al  Kilburn & Strode 30, John Street London WC1N 2DD London WC1N 2DD (GB)