(19)
(11) EP 0 320 483 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
09.08.1989 Bulletin 1989/32

(43) Date of publication A2:
14.06.1989 Bulletin 1989/24

(21) Application number: 88870182.8

(22) Date of filing: 08.12.1988
(51) International Patent Classification (IPC)4C12N 11/04, A01N 63/00
// (C12N11/04, C12R1:07, 1:38)
(84) Designated Contracting States:
AT BE CH DE ES FR GB GR IT LI LU NL SE

(30) Priority: 11.12.1987 US 131965

(71) Applicant: Monsanto Company
St. Louis Missouri 63167-7020 (US)

(72) Inventors:
  • Baker, Carol Ann
    St. Louis Missouri 63146 (US)
  • Brooks, Albert Arthur
    St. Louis Missouri 63136 (US)
  • Greenley, Robert Ziolkowski
    Frontenac Missouri 63131 (US)
  • Henis, Jay Myls Stuart
    Creve Coeur Missouri 63141 (US)

(74) Representative: Ernst, Hubert et al
Monsanto Services International S.A. Patent Department Avenue de Tervuren 270-272 Letter Box No. 21
1150 Bruxelles
1150 Bruxelles (BE)


(56) References cited: : 
   
       


    (54) Encapsulation method


    (57) Biological material such as proteins and microbes is encapsulated by a method which comprises

    a) admixing the biological material with an aqueous non-ionic polymer solution in which the polymer is present at a concentration of at least 3% w/v;

    b) forming polymeric beads by adding the mixture of part (a) dropwise into a water-immiscible non-solvent for said polymer, said non-solvent being maintained at a temperature sufficient to freeze the bead but not so low to cause freeze fracture; and

    c) drying the beads of step (b) to remove substantially all unbound water in said beads.


    Freeze-drying produces a stable encapsulated product which in the case of microbes retains a high level of viability. The method provides a means of using microbes as agricultural agents since the encapsulated product is acceptable and useful to the farmer.





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