| (19) |
 |
|
(11) |
EP 0 320 483 A3 |
| (12) |
EUROPEAN PATENT APPLICATION |
| (88) |
Date of publication A3: |
|
09.08.1989 Bulletin 1989/32 |
| (43) |
Date of publication A2: |
|
14.06.1989 Bulletin 1989/24 |
| (22) |
Date of filing: 08.12.1988 |
|
| (51) |
International Patent Classification (IPC)4: C12N 11/04, A01N 63/00 // (C12N11/04, C12R1:07, 1:38) |
|
| (84) |
Designated Contracting States: |
|
AT BE CH DE ES FR GB GR IT LI LU NL SE |
| (30) |
Priority: |
11.12.1987 US 131965
|
| (71) |
Applicant: Monsanto Company |
|
St. Louis
Missouri 63167-7020 (US) |
|
| (72) |
Inventors: |
|
- Baker, Carol Ann
St. Louis
Missouri 63146 (US)
- Brooks, Albert Arthur
St. Louis
Missouri 63136 (US)
- Greenley, Robert Ziolkowski
Frontenac
Missouri 63131 (US)
- Henis, Jay Myls Stuart
Creve Coeur
Missouri 63141 (US)
|
| (74) |
Representative: Ernst, Hubert et al |
|
Monsanto Services International S.A.
Patent Department
Avenue de Tervuren 270-272
Letter Box No. 21 1150 Bruxelles 1150 Bruxelles (BE) |
|
| |
|
| (54) |
Encapsulation method |
(57) Biological material such as proteins and microbes is encapsulated by a method which
comprises
a) admixing the biological material with an aqueous non-ionic polymer solution in
which the polymer is present at a concentration of at least 3% w/v;
b) forming polymeric beads by adding the mixture of part (a) dropwise into a water-immiscible
non-solvent for said polymer, said non-solvent being maintained at a temperature sufficient
to freeze the bead but not so low to cause freeze fracture; and
c) drying the beads of step (b) to remove substantially all unbound water in said
beads.
Freeze-drying produces a stable encapsulated product which in the case of microbes
retains a high level of viability. The method provides a means of using microbes as
agricultural agents since the encapsulated product is acceptable and useful to the
farmer.