(19)
(11) EP 0 322 032 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
30.01.1991 Bulletin 1991/05

(43) Date of publication A2:
28.06.1989 Bulletin 1989/26

(21) Application number: 88202878.0

(22) Date of filing: 14.12.1988
(51) International Patent Classification (IPC)4: C12P 35/02, C12N 9/80, C12N 15/00, C12N 1/20
// (C12N1/20, C12R1:11),(C12N9/80, C12R1:11),(C12P35/02, C12R1:11)
(84) Designated Contracting States:
CH DE FR GB IT LI NL

(30) Priority: 21.12.1987 US 136226

(71) Applicant: MERCK & CO. INC.
Rahway New Jersey 07065-0900 (US)

(72) Inventor:
  • Lein, Joseph
    Deer Harbor Washington (US)

(74) Representative: Cole, William Gwyn 
European Patent Department Merck & Co., Inc. Terlings Park Eastwick Road
Harlow Essex CM20 2QR
Harlow Essex CM20 2QR (GB)


(56) References cited: : 
   
       


    (54) One-step enzymatic conversion of cephalosporin C and derivatives to 7-aminocephalosporanic acid and derivatives


    (57) A process for the one step conversion of cephalosporin C and derivatives thereof to the corresponding 7-aminocephalosporanic acid and derivatives comprising treating said cephalosporin C and derivatives with a cephalosporin C amidase derived from Bacillus megaterium ATCC 53667, or from any cephalosporin C amidase producing, or potentially producing descendants thereof, or from any expression of the genetic material of said Bacillus megaterium ATCC 53667, or any cephalosporin C amidase producing, or potentially producing descendants thereof.





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