| (19) |
 |
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(11) |
EP 0 330 191 A3 |
| (12) |
EUROPEAN PATENT APPLICATION |
| (88) |
Date of publication A3: |
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16.08.1990 Bulletin 1990/33 |
| (43) |
Date of publication A2: |
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30.08.1989 Bulletin 1989/35 |
| (22) |
Date of filing: 23.02.1989 |
|
| (51) |
International Patent Classification (IPC)4: C12N 15/00 |
|
| (84) |
Designated Contracting States: |
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AT BE CH DE ES FR GB IT LI LU NL SE |
| (30) |
Priority: |
25.02.1988 US 160416
|
| (60) |
Divisional application: |
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96104493.0 |
| (71) |
Applicant: THE GENERAL HOSPITAL CORPORATION |
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Boston, MA 02114 (US) |
|
| (72) |
Inventors: |
|
- Seed, Brian, Dr.
Department of Molecular Biology
Boston, MA 02114 (US)
- Allen, Janet
Boston, MA 02116 (US)
- Aruffo, Alejandro
Belmont, MA 02178 (US)
- Camerini, David
Cambridge, MA 02108 (US)
- Lauffer, Leander, Dr.
Boston, MA 02108 (US)
- Oquendo, Carmen Patricia
Boston, MA 02108 (US)
- Simmons, David
Boston, MA 02114 (US)
- Stamenkovic, Ivan
Brookline, MA 02146 (US)
- Stengelin, Siegfried, Dr.
D-6238 Hofheim am Taunus (DE)
|
| (74) |
Representative: Fischer, Hans-Jürgen, Dr. et al |
|
Hoechst AG
Patent- und Lizenzabteilung
Gebäude K 801
65926 Frankfurt am Main
65926 Frankfurt am Main (DE) |
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| |
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(57) A simple and highly efficient method for cloning cDNAs from mammalian expression
libraries based on transient expression in mammalian host cells has been discovered.
Novel expression vectors allowing highly efficient construction of mammalian cDNA
libraries are disclosed. The cloning method of the invention which has been used to
clone genes for cell surface antigens of human lymphocytes, has general application
in gene cloning. Cell surface antigens cloned according to the present invention have
been purified, and the nucleotide and amino acid sequences determined. These antigens
have diagnostic and therapeutic utility in immune-mediated infections in mammals,
including humans.