| (19) |
 |
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(11) |
EP 0 417 841 A3 |
| (12) |
EUROPEAN PATENT APPLICATION |
| (88) |
Date of publication A3: |
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18.09.1991 Bulletin 1991/38 |
| (43) |
Date of publication A2: |
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20.03.1991 Bulletin 1991/12 |
| (22) |
Date of filing: 06.09.1990 |
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| (84) |
Designated Contracting States: |
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AT BE CH DE DK FR GB IT LI LU NL SE |
| (30) |
Priority: |
12.09.1989 US 406224
|
| (71) |
Applicant: EASTMAN KODAK COMPANY |
|
Rochester,
New York 14650-2201 (US) |
|
| (72) |
Inventors: |
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- Warren III, Harold Chester,
c/o EASTMAN KODAK COMP
Rochester,
New York 14650 (US)
- Oakes, Fred Terry,
c/o EASTMAN KODAK COMPANY
Rochester,
New York 14650 (US)
|
| (74) |
Representative: Nunney, Ronald Frederick Adolphe et al |
|
Kodak Limited
Patent Department
Headstone Drive
Harrow
Middlesex HA1 4TY
Harrow
Middlesex HA1 4TY (GB) |
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| |
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Remarks: |
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The applicant has subsequently filed a sequence listing and declared, that it includes
no new matter. |
|
| (54) |
An oligonucleotide-enzyme conjugate and a method for its preparation |
(57) A method is provided for preparing a covalent conjugate of an oligonucleotide and
an enzyme, such as peroxidase. This conjugate can be used as a probe in hybridization
assays and in polymerase chain reaction procedures. The method generally comprises
the steps of: reacting an enzyme having a reactive amino group with a mercapto-substituted
organic compound to form a blocked intermediate, removing the blocking group to form
an enzyme reagent, and reacting the enzyme reagent with a functionalized oligonucleotide
reagent.