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(11)

EP 0 436 634 A1

(12)

(43) Date of publication: 17.07.1991 Bulletin 1991/29 (21) Application number: 89911309.0 (22) Date of filing: 29.09.1989

(51) International Patent Classification (IPC):  A61K 38/ 00( . ) A61K 39/ 395( . ) A61K 49/ 00( . ) C07K 7/ 06( . ) C07K 14/ 16( . ) C07K 19/ 00( . ) C12P 21/ 02( . ) G01N 33/ 569( . ) A61K 39/ 00( . ) A61K 39/ 21( . ) A61K 39/ 42( . ) C07K 5/ 10( . ) C07K 7/ 08( . ) C07K 16/ 00( . ) C12N 15/ 09( . ) C12P 21/ 08( . ) G01N 33/ 68( . ) C12R 1/ 19( . ) (86) International application number: PCT/US1989/004302 (87) International publication number: WO 1990/003984 (19.04.1990 Gazette 1990/09)

(84) Designated Contracting States: AT BE CH DE FR GB IT LI LU NL SE (30) Priority: 03.10.1988 US 19880252949 01.06.1989 US 19890359543 19.09.1989 US 19890407663 (71) Applicant: REPLIGEN CORPORATION Cambridge, MA 02139 (US)

(72) Inventors: RUSCHE, James, R. Framingham, MA 01701 (US) PUTNEY, Scott, D. Arlington, MA 02174 (US) JAVAHERIAN, Kashayar Lexington, MA 02173 (US) FARLEY, John Pittsford, NY 14534 (US) GRIMAILA, Raymond Somerville, MA 02134 (US) LYNN, Debra Arlington, MA 02174 (US) PETRO-BREYER, J. Inst.Pasteur F-75724 Paris Ced. 1515 (FR) O'KEEFFE, Thomas Windham, NH 03089 (US) LAROSA, Gregory Brookline, MA 02146 (US) PROFY, Albert, T. Cambridge, MA 02139 (US) (74) Representative: Deans, Michael John Percy  Lloyd Wise, Tregear & CO. Norman House 105-109 Strand London WC2R OAE London WC2R OAE (GB)