(19)
(11) EP 0 468 262 A3

(12) EUROPEAN PATENT APPLICATION

(88) Date of publication A3:
03.06.1992 Bulletin 1992/23

(43) Date of publication A2:
29.01.1992 Bulletin 1992/05

(21) Application number: 91111306.6

(22) Date of filing: 08.07.1991
(51) International Patent Classification (IPC)5: C12N 9/96, C12Q 1/32, C12Q 1/527
(84) Designated Contracting States:
BE CH DE FR GB IT LI NL

(30) Priority: 20.07.1990 US 554512

(71) Applicant: MERCK PATENT GmbH
D-64271 Darmstadt (DE)

(72) Inventors:
  • Crowther, Fredric H.
    Chadds Ford, PA 19317 (US)
  • Law, Wai Tak
    Sewell, NJ 08080 (US)


(56) References cited: : 
   
       


    (54) Reagents for CO 2 detection


    (57) A stabilizer for maintaining a constant level of C02 in coenzyme-containing enzymatic C02-diagnostic reagents is disclosed,comprising rate-limiting amounts of (a) the diagnostic reagents and (b) coenzyme- regenerating reagents, e.g., wherein (a) is malate dehydrogenase, phosphoenolpyruvate carboxylase and NADH, and (b) is glucose dehydrogenase and glucose, as are methods of use and kits containing a diagnostic reagent and stabilizer.






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