(19)

(11)

EP 0 483 269 A1

(12)

(43) Date of publication: 06.05.1992 Bulletin 1992/19 (21) Application number: 90911812.0 (22) Date of filing: 18.07.1990

(51) International Patent Classification (IPC):  C12N 9/ 22( . ) C12N 15/ 54( . ) (86) International application number: PCT/US1990/004026 (87) International publication number: WO 1991/001371 (07.02.1991 Gazette 1991/04)

(84) Designated Contracting States: AT BE CH DE DK ES FR GB IT LI LU NL SE (30) Priority: 18.07.1989 US 19890381460 18.07.1989 US 19890381472 (71) Applicants: COLD SPRING HARBOR LABORATORY Cold Spring Harbor, NY 11724 (US) RUTGERS UNIVERSITY Piscataway, NJ 08855-1179 (US)	(72) Inventors: ROBERTS, Richard, J. Cold Spring Harbor, NY 11724 (US) MEYERTONS, Janise, L. Centerport, NY 11721 (US) LECHEVALIER, Mary, P. Piscataway, NJ 00854 (US) (74) Representative: Paget, Hugh Charles Edward, et al  MEWBURN ELLIS York House 23 Kingsway London WC2B 6HP London WC2B 6HP (GB)