(19)
(11)
EP 0 503 045 A1
(12)
(43)
Date of publication:
16.09.1992
Bulletin 1992/38
(21)
Application number:
91917904.0
(22)
Date of filing:
22.08.1991
(51)
International Patent Classification (IPC):
C12N
1/
21
( . )
C12N
15/
09
( . )
C12N
15/
78
( . )
C12R
1/
01
( . )
C12N
9/
02
( . )
C12N
15/
74
( . )
C12P
21/
02
( . )
(86)
International application number:
PCT/US1991/006006
(87)
International publication number:
WO 1992/006186
(
16.04.1992
Gazette 1992/09)
(84)
Designated Contracting States:
AT BE CH DE DK ES FR GB GR IT LI LU NL SE
(30)
Priority:
28.09.1990
US 19900590280
(71)
Applicant:
AMGEN INC.
Thousand Oaks, CA 91320-1789 (US)
(72)
Inventor:
YEN, Kwang-Mu
Thousand Oaks, CA 91360 (US)
(74)
Representative:
Brown, John David, et al
FORRESTER & BOEHMERT Franz-Joseph-Strasse 38
D-80801 München
D-80801 München (DE)
(54)
CLONING CARTRIDGES AND EXPRESSION VECTORS IN GRAM-NEGATIVE BACTERIA