(19)

(11)

EP 0 537 259 A1

(12)

(43) Date of publication: 21.04.1993 Bulletin 1993/16 (21) Application number: 91912786.0 (22) Date of filing: 04.07.1991

(51) International Patent Classification (IPC):  C12P 7/ 40( . ) C12R 1/ 01( . ) C12R 1/ 38( . ) C12R 1/ 425( . ) C12P 41/ 00( . ) C12R 1/ 13( . ) C12R 1/ 40( . ) (86) International application number: PCT/DK1991/000189 (87) International publication number: WO 1992/001062 (23.01.1992 Gazette 1992/03)

(84) Designated Contracting States: AT BE CH DE DK ES FR GB GR IT LI LU NL SE (30) Priority: 05.07.1990 DK 19900001616 (71) Applicants: NOVO NORDISK A/S DK-2880 Bagsvaerd (DK) E.I. DU PONT DE NEMOURS AND COMPANY Wilmington Delaware 19898 (US)

(72) Inventors: STIEGLITZ, Barry Overbrook Hills, PA 19151 (US) LINN, William, J. Wilmington, DE 19810 (US) JOBST, Wolfram Wilmington, DE 19806 (US) FRIED, Karen, M. Voorhees, NJ 08043 (US) FALLON, Robert, D. Elkton, MD 21921 (US) INGVORSEN, Kjeld DK-3500 Vaerloese (DK) YDE, Birgitte DK-2900 Hellerup (DK) (74) Representative: Noergaard, Torsten  c/o Novo Nordisk A/S Novo Allé DK-2880 Bagsvaerd DK-2880 Bagsvaerd (DK)