| (19) |
 |
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(11) |
EP 0 682 106 A3 |
| (12) |
EUROPEAN PATENT APPLICATION |
| (88) |
Date of publication A3: |
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19.11.1997 Bulletin 1997/47 |
| (43) |
Date of publication A2: |
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15.11.1995 Bulletin 1995/46 |
| (22) |
Date of filing: 11.04.1995 |
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| (84) |
Designated Contracting States: |
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CH DE FR GB LI |
| (30) |
Priority: |
11.04.1994 JP 98056/94
23.08.1994 JP 198838/94
|
| (71) |
Applicant: RESEARCH DEVELOPMENT CORPORATION OF JAPAN |
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Kawaguchi-shi,
Saitama-ken 332 (JP) |
|
| (72) |
Inventors: |
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- Tateno, Chise
Higashi-Hiroshima-shi,
Hiroshima, (JP)
- Yoshizato, Katsutoshi
Higashi-Hiroshima-shi,
Hiroshima, (JP)
|
| (74) |
Representative: Hildyard, Edward Martin |
|
Frank B. Dehn & Co.
Imperial House
15-19 Kingsway
London WC2B 6UZ
London WC2B 6UZ (GB) |
|
| |
|
| (54) |
Liver parenchymal cells having clonal growth ability, method for obtaining same, method
for culturing same, and culturing system of primary hepatocytes |
(57) The present invention provides liver parenchymal cells having a clonal growth ability,
which possesses at least one of the cell biological properties such as: presence of
peroxysome; being positive to hepatocyte-markers; being partially positive to neoplastic
hepatocyte-markers or immature hepatocyte-markers; being positive to antibodies against
the surface antigens of ovall cells; and being partially positive to bile duct cell-markers.
The present invention also provides a method for obtaining such cells and a method
for subculturlng such cells.
With the liver parenchymal cells above, it will be possible to research in detail
the process of development and differentiation, the mechanisms of growth and functional
expression of hepatic cells, and to open up a new way to clalification of mechanisms
of hepatoma and various other diseases and to development of therapeutic method against
these disseases.