Compositions and methods for the simultaneous detection and quantification of multiple specific nucleic acid sequences

(19)

(11)

EP 0 709 466 A3

(12)	EUROPEAN PATENT APPLICATION

(88)	Date of publication A3:
	12.06.1996 Bulletin 1996/24

(43)	Date of publication A2:
	01.05.1996 Bulletin 1996/18

(21)	Application number: 95116199.1

(22)	Date of filing: 13.10.1995

(51)	International Patent Classification (IPC)⁶: C12Q 1/68, C07D 219/00, C07H 21/00

(84)	Designated Contracting States:
	AT BE CH DE DK ES FR GB IT LI LU NL SE

(30)

Priority:

28.10.1994 US 331107

(71)	Applicant: GEN-PROBE INCORPORATED
	San Diego California 92121-1514 (US)

(72)	Inventors:
	Nelson, Norman C. San Diego, California 92111 (US) Woodhead, James S. Raglan NP5 2LD (GB) Weeks, Ian Penylan Cardiff CF3 9DJ (GB) Cheikh, Azzouz Ben California 92014 (US)

(74)	Representative: Patentanwälte Viering, Jentschura & Partner
	Postfach 22 14 43 D-80504 München D-80504 München (DE)

(54)	Compositions and methods for the simultaneous detection and quantification of multiple specific nucleic acid sequences

(57) The invention relates to methods for simultaneously or sequentially detecting multiple nucleic acid analytes in a single medium utilizing oligonucleotide hybridization probes coupled to different chemiluminescent labeling reagents. The methods may be used in a heterogeneous, homogeneous or non-homogeneous assay system. The invention also relates to specific combinations of chemiluminescent labeling reagents suitable, when coupled to an oligonucleotide probe, for use together in methods for the detection of multiple nucleic acid analytes. The invention also concerns kits useful in these methods.

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