| (19) |
 |
|
(11) |
EP 0 737 751 A3 |
| (12) |
EUROPEAN PATENT APPLICATION |
| (88) |
Date of publication A3: |
|
29.01.1997 Bulletin 1997/05 |
| (43) |
Date of publication A2: |
|
16.10.1996 Bulletin 1996/42 |
| (22) |
Date of filing: 12.04.1996 |
|
| (51) |
International Patent Classification (IPC)6: C12P 7/62, C12N 1/14
// (C12N1/14, C12R1:645),(C12P7/62, C12R1:645) |
|
| (84) |
Designated Contracting States: |
|
CH DE ES FR GB IT LI |
| (30) |
Priority: |
13.04.1995 JP 87934/95
08.04.1996 JP 85517/96
|
| (71) |
Applicant: Mitsubishi Chemical Corporation |
|
Chiyoda-ku,
Tokyo (JP) |
|
| (72) |
Inventors: |
|
- Yamagishi, Masahiro,
c/o Mitsubishi Chem. Corp.
Yokohama,
Kanagawa (JP)
- Ueda, Makoto,
c/o Mitsubishi Chem. Corp.
Yokohama,
Kanagawa (JP)
- Takai, Yukie,
c/o Mitsubishi Chem. Corp.
Yokohama,
Kanagawa (JP)
- Yasuda, Mari,
c/o Mitsubishi Chem. Corp.
Yokohama,
Kanagawa (JP)
- Mikawa, Takashi,
c/o Mitsubishi Chem. Corp.
Yokohama,
Kanagawa (JP)
|
| (74) |
Representative: Kolb, Helga, Dr. Dipl.-Chem. et al |
|
Hoffmann, Eitle & Partner,
Patent-und Rechtsanwälte,
Arabellastrasse 4
81925 München
81925 München (DE) |
|
| |
|
| (54) |
Method for producing optically active ester of gamma-substituted-beta-hydroxybutyric
acid |
(57) Microbial cells and/or a preparation therefrom of a microorganism is allowed to act
on an ester of γ-substituted-acetoacetic acid, and a carbonyl group at its β-position
is stereospecifically reduced, wherein the microorganism is selected from the group
consisting of those belonging to the genera
Yarrowia,
Filobasidium,
Metschnikowia,
Galactomyces,
Ambrosiozyma,
Trichosporonoides,
Aureobasidium,
Phaeococcomyces,
Rosulomyces,
Dothichiza,
Emericellopsis,
Calonectria,
Colletotrichum, and
Ceratocystis, preferably, the microorganism is microorganism belonging to the genus
Aureobasidium. Thus an optically active ester of γ-substituted-β-hydroxybutyric acid having a high
optical purity is produced in a short period of time at a high accumulated concentration
and a high yield.