Preparation of D-alpha-amino acids, using bacteria transformed with the carbamoylase-hydantoinase operon

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EP 0 775 748 A3

(12)	EUROPEAN PATENT APPLICATION

(88)	Date of publication A3:
	07.04.1999 Bulletin 1999/14

(43)	Date of publication A2:
	28.05.1997 Bulletin 1997/22

(21)	Application number: 96117455.4

(22)	Date of filing: 31.10.1996

(51)	International Patent Classification (IPC)⁶: C12N 15/52, C12P 13/04, C12N 1/20 // C12P41/00 ,(C12N1/20, C12R1:19, 1:125)

(84)	Designated Contracting States:
	AT BE CH DE DK ES FI FR GB GR IE LI LU NL PT SE

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Priority:

23.11.1995 IT MI952432

(71)	Applicant: ENIRICERCHE S.p.A.
	20097 S. Donato Milanese (Milano) (IT)

(72)	Inventors:
	Grifantini, Renata Milano (IT) Galli, Giuliano 20097 S. Donato Milanese (IT) Grandi, Guido 20090 Segrate (s. Felice) (Milano) (IT) Carpani, Giovanna 26010 Sergnano (CR) (IT)

(74)	Representative: Gennari, Marco
	Eniricerche S.p.A., BREL, Via F. Maritano, 26 20097 San Donato Milanese (MI) 20097 San Donato Milanese (MI) (IT)

(54)	Preparation of D-alpha-amino acids, using bacteria transformed with the carbamoylase-hydantoinase operon

(57) In an improved process for the preparation of D-α-amino acids by the stereoselective conversion of racemic mixtures of 5-substituted hydantoins with an enzymatic system produced by a microorganism, the improvement consists in the fact that a microorganism is used transformed with the plasmid pSM700 cultivated at a temperature of between 20°C and 28°C.
The use of this microorganism allows an improvement in the expression of the enzymatic system and an increase in the conversion rate of the racemic hydantoin to D-α-amino acid.

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